AlfalfaGEDB | MsaG026730

M. sativa ssp. caerulea / MsaG026730

KEGG: -
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046983 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0097159 GO:1901363
PFAM: PF04770.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026730	XP_003615230.1	84.387	269	12	3	14	253	1	268	5.45e-132	384

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026730	sp\|Q9SB61\|ZHD2_ARATH	45.122	246	94	6	20	253	4	220	1.04e-53	176

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026730	tr\|G7KBF3\|G7KBF3_MEDTR	84.387	269	12	3	14	253	1	268	2.60e-132	384

TFs/TRs:

Gene ID	Type	Classification
MsaG026730	TF	zf-HD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG007404	MsaG026730	0.959347	8.616428e-117	4.261515e-111
MsaG007405	MsaG026730	0.914430	4.791702e-84	6.088199e-80
MsaG007406	MsaG026730	0.945259	1.309427e-103	1.974803e-98
MsaG010191	MsaG026730	0.874261	1.600700e-67	2.228637e-64
MsaG012005	MsaG026730	0.896049	1.208880e-75	4.932152e-72
MsaG012006	MsaG026730	0.887051	4.348654e-72	1.102208e-68
MsaG023394	MsaG026730	0.956023	2.666973e-113	9.935872e-108
MsaG023596	MsaG026730	0.941103	2.203218e-100	2.273731e-95
MsaG023597	MsaG026730	0.920217	4.332365e-87	8.300792e-83
MsaG023600	MsaG026730	0.949662	2.586089e-107	5.696060e-102
MsaG026693	MsaG026730	0.807607	7.912370e-50	1.275488e-47
MsaG026729	MsaG026730	0.922351	2.856245e-88	6.400435e-84
MsaG026728	MsaG026730	0.942261	2.942621e-101	3.399601e-96
MsaG026730	MsaG041869	0.801855	1.240433e-48	1.749040e-46
MsaG026730	MsaG046085	0.868136	1.650291e-65	1.770214e-62
MsaG026730	MsaG008731	0.910435	4.553331e-82	4.424563e-78
MsaG026730	MsaG027221	0.868942	9.085624e-66	1.007361e-62
MsaG026730	MsaG026405	0.929325	2.216797e-92	8.535814e-88
MsaG026730	MsaG037887	0.924033	3.170348e-89	8.054509e-85

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026730	MtrunA17_Chr5g0427051	84.387	269	12	3	14	253	1	268	5.02e-136	384
MsaG026730	MtrunA17_Chr3g0133181	56.148	244	73	4	33	249	34	270	2.83e-76	232
MsaG026730	MtrunA17_Chr5g0412501	46.226	212	69	6	71	253	77	272	2.59e-42	145
MsaG026730	MtrunA17_Chr1g0188441	39.474	190	100	4	69	252	6	186	5.34e-37	129
MsaG026730	MtrunA17_Chr4g0076821	36.441	236	95	6	71	252	112	346	5.08e-35	128
MsaG026730	MtrunA17_Chr7g0217231	35.814	215	90	4	69	252	85	282	8.57e-33	122
MsaG026730	MtrunA17_Chr2g0315941	36.000	200	109	3	72	252	68	267	2.01e-31	117
MsaG026730	MtrunA17_Chr5g0404271	34.722	216	95	6	68	252	95	295	1.25e-30	115
MsaG026730	MtrunA17_Chr8g0367691	35.323	201	110	4	71	252	68	267	1.12e-28	109
MsaG026730	MtrunA17_Chr6g0479901	33.708	178	81	4	72	248	19	160	1.69e-21	88.6
MsaG026730	MtrunA17_Chr8g0357741	54.667	75	33	1	178	252	23	96	1.75e-20	83.2
MsaG026730	MtrunA17_Chr7g0243651	86.842	38	5	0	70	107	1	38	1.28e-18	77.8
MsaG026730	MtrunA17_Chr8g0361671	52.381	63	30	0	190	252	271	333	7.51e-18	82.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026730	AT4G24660.1	45.122	246	94	6	20	253	4	220	1.06e-54	176
MsaG026730	AT4G24660.2	44.215	242	94	6	20	249	4	216	1.61e-51	168
MsaG026730	AT2G02540.2	37.398	246	126	7	31	252	43	284	2.90e-46	157
MsaG026730	AT2G02540.1	37.398	246	126	7	31	252	43	284	2.90e-46	157
MsaG026730	AT5G65410.1	43.299	194	102	3	63	253	66	254	1.25e-40	141
MsaG026730	AT1G14440.1	38.265	196	105	4	70	252	88	280	3.91e-38	135
MsaG026730	AT1G14440.2	38.265	196	105	4	70	252	88	280	3.91e-38	135
MsaG026730	AT2G18350.1	39.247	186	103	3	71	252	81	260	8.12e-36	128
MsaG026730	AT3G50890.1	39.791	191	98	5	71	251	59	242	1.54e-33	122
MsaG026730	AT5G15210.1	36.548	197	96	4	67	246	51	235	5.53e-28	108
MsaG026730	AT1G69600.1	36.150	213	77	5	70	249	29	215	1.31e-27	106
MsaG026730	AT5G60480.1	38.378	185	92	7	69	246	34	203	5.56e-27	104
MsaG026730	AT5G39760.1	58.462	65	27	0	188	252	198	262	3.37e-20	88.6
MsaG026730	AT3G28920.1	59.677	62	25	0	188	249	190	251	1.01e-19	86.7
MsaG026730	AT5G42780.1	26.816	179	126	3	72	249	64	238	1.66e-14	71.2
MsaG026730	AT1G74660.2	48.276	58	30	0	67	124	34	91	4.14e-12	61.2
MsaG026730	AT1G74660.1	67.500	40	13	0	67	106	34	73	8.28e-12	60.8

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGCACTGGTGTTTGTGTTTC+TGG	0.083748	5:-27560726	None:intergenic
AGAGAAATGTTCTTAAAGTT+TGG	0.256503	5:+27561413	MsaT026730.1:CDS
GGTGGTGGTGGTAGTGGTGG+TGG	0.308191	5:+27561250	MsaT026730.1:CDS
GGAGGTGGTGGTGGTAGTGG+TGG	0.318570	5:+27561247	MsaT026730.1:CDS
GCTCTCCCTGCTGCTGCTTC+CGG	0.319955	5:+27561169	MsaT026730.1:CDS
GGAGGAGGTGGTGGTGGTAG+TGG	0.325754	5:+27561244	MsaT026730.1:CDS
CCTGCTGCTGCTTCCGGTGG+TGG	0.355404	5:+27561175	MsaT026730.1:CDS
GGTGGTGGTAGTGGTGGTGG+TGG	0.415280	5:+27561253	MsaT026730.1:CDS
CCACCACCGGAAGCAGCAGC+AGG	0.427582	5:-27561175	None:intergenic
AAGAATCACGCCGTTAGCTT+CGG	0.428386	5:+27560870	MsaT026730.1:CDS
TTTATCGCTGCCGGTGAAGA+AGG	0.429195	5:+27560921	MsaT026730.1:CDS
TGGTGATGGTATTGCGGCGG+TGG	0.436665	5:-27561043	None:intergenic
AGTAGCGGTGGAGGAGGTGG+TGG	0.442867	5:+27561235	MsaT026730.1:CDS
AAACTCACCGTCGGAGTTGA+CGG	0.443498	5:+27560789	MsaT026730.1:CDS
AGCAGTAGCGGTGGAGGAGG+TGG	0.444551	5:+27561232	MsaT026730.1:CDS
GTAGTGGTGGTGGTGGATCA+AGG	0.444962	5:+27561260	MsaT026730.1:CDS
TTCGGTGGCCATGCTGTTGA+CGG	0.447816	5:+27560888	MsaT026730.1:CDS
CACAATAACAAGAACACTCT+TGG	0.453033	5:+27561439	MsaT026730.1:CDS
GGAGGAGCACGGTGGTAGTA+AGG	0.453304	5:-27561082	None:intergenic
CGTGATTCTTCTGACATTCT+CGG	0.457636	5:-27560857	None:intergenic
TGATGGTATTGCGGCGGTGG+AGG	0.460852	5:-27561040	None:intergenic
GGAACAGAAAGAGAAGTTGC+TGG	0.466315	5:+27561309	MsaT026730.1:CDS
CCTCCTCCACCGCTACTGCT+AGG	0.470793	5:-27561229	None:intergenic
AGCGGTGGAGGAGGTGGTGG+TGG	0.484419	5:+27561238	MsaT026730.1:CDS
TATTGCGGCGGTGGAGGTGA+TGG	0.492574	5:-27561034	None:intergenic
AACGGTCTGTGATGTGCCAC+CGG	0.497928	5:-27561145	None:intergenic
ATGATGGTGGTGAAGGTAAC+CGG	0.500422	5:-27561113	None:intergenic
AGAGGAACAAGAAGAGGAGA+TGG	0.506205	5:+27560698	MsaT026730.1:CDS
TTCAAGTGTTCCTTCTTCAC+CGG	0.506613	5:-27560931	None:intergenic
CGTGCTCCTCCTTCCACAGC+CGG	0.511664	5:+27561094	MsaT026730.1:CDS
TTCTCTGCTCATTCCACCAC+CGG	0.513977	5:-27561188	None:intergenic
AACCCTAGCAGTAGCGGTGG+AGG	0.514729	5:+27561226	MsaT026730.1:CDS
CTCCTCCACCGCTACTGCTA+GGG	0.520409	5:-27561228	None:intergenic
CTCCCTGCTGCTGCTTCCGG+TGG	0.523308	5:+27561172	MsaT026730.1:CDS
GCAGCAGCAGGGAGAGCCAA+CGG	0.524507	5:-27561163	None:intergenic
ACAGAGCAGCGCGAGATGCT+AGG	0.535949	5:+27560250	MsaT026730.1:CDS
AATCACGCCGTTAGCTTCGG+TGG	0.536570	5:+27560873	MsaT026730.1:CDS
TTTCCTTCCGTCAACTCCGA+CGG	0.536586	5:-27560796	None:intergenic
GAGTCATAACTCGTTGGCAC+TGG	0.537593	5:-27560741	None:intergenic
CCTAGCAGTAGCGGTGGAGG+AGG	0.539653	5:+27561229	MsaT026730.1:CDS
CCACCATCATCTTGCAACTC+CGG	0.539922	5:+27561126	MsaT026730.1:CDS
GTGGTGAAGGTAACCGGCTG+TGG	0.551358	5:-27561107	None:intergenic
GTCGGAGTTGACGGAAGGAA+AGG	0.556014	5:+27560798	MsaT026730.1:CDS
TTGCAAGATGATGGTGGTGA+AGG	0.558218	5:-27561120	None:intergenic
AATAGAATTTGATGAACAAG+AGG	0.563290	5:+27560680	MsaT026730.1:CDS
TGTTGCGAGTTTATCGCTGC+CGG	0.567002	5:+27560912	MsaT026730.1:CDS
CCACCGGAGTTGCAAGATGA+TGG	0.569041	5:-27561129	None:intergenic
TGAAGGTAACCGGCTGTGGA+AGG	0.577589	5:-27561103	None:intergenic
AGAATTGATTGCTGTGGTGA+TGG	0.582941	5:-27561057	None:intergenic
TGAACAAGAGGAACAAGAAG+AGG	0.593455	5:+27560692	MsaT026730.1:CDS
CACCACCGGAAGCAGCAGCA+GGG	0.602778	5:-27561174	None:intergenic
CCAACGAGTTATGACTCACT+TGG	0.611073	5:+27560747	MsaT026730.1:CDS
CGCAACAACCGTCAACAGCA+TGG	0.612162	5:-27560896	None:intergenic
AGGTAACCGGCTGTGGAAGG+AGG	0.612370	5:-27561100	None:intergenic
GGTGGCACATCACAGACCGT+TGG	0.612785	5:+27561147	MsaT026730.1:CDS
ATGTCAAACCCTAGCAGTAG+CGG	0.624015	5:+27561220	MsaT026730.1:CDS
ATTCAGAACAAAATTCACTC+AGG	0.624697	5:+27561288	MsaT026730.1:CDS
TTGCTGTGGTGATGGTATTG+CGG	0.630881	5:-27561049	None:intergenic
TCAAACCCTAGCAGTAGCGG+TGG	0.637366	5:+27561223	MsaT026730.1:CDS
GGAAGGAAAGGTAACGGCAA+CGG	0.639375	5:+27560810	MsaT026730.1:CDS
TCACCGTCGGAGTTGACGGA+AGG	0.639683	5:+27560793	MsaT026730.1:CDS
CCATCATCTTGCAACTCCGG+TGG	0.641524	5:+27561129	MsaT026730.1:CDS
CCAAGTGAGTCATAACTCGT+TGG	0.644449	5:-27560747	None:intergenic
GAGCAGCGCGAGATGCTAGG+AGG	0.657086	5:+27560253	MsaT026730.1:CDS
CGGCTGTGGAAGGAGGAGCA+CGG	0.673027	5:-27561093	None:intergenic
AGCATGGCCACCGAAGCTAA+CGG	0.680090	5:-27560880	None:intergenic
GTTGACGGAAGGAAAGGTAA+CGG	0.682718	5:+27560804	MsaT026730.1:CDS
TCACGTTCAAAACTCACCGT+CGG	0.687077	5:+27560780	MsaT026730.1:CDS
CTGTGGTGATGGTATTGCGG+CGG	0.691893	5:-27561046	None:intergenic
CCGGAGTTGCAAGATGATGG+TGG	0.694873	5:-27561126	None:intergenic
AGGAAAGGTAACGGCAACGG+CGG	0.697533	5:+27560813	MsaT026730.1:CDS
AAGGCGAGAATTGATTGCTG+TGG	0.722344	5:-27561063	None:intergenic
CTGTGGAAGGAGGAGCACGG+TGG	0.754237	5:-27561090	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	27560240	27561474	27560240	ID=MsaG026730
Chr5	mRNA	27560240	27561474	27560240	ID=MsaT026730.1;Parent=MsaG026730
Chr5	exon	27560240	27560274	27560240	ID=MsaT026730.1.exon1;Parent=MsaT026730.1
Chr5	CDS	27560240	27560274	27560240	ID=cds.MsaT026730.1;Parent=MsaT026730.1
Chr5	exon	27560677	27560962	27560677	ID=MsaT026730.1.exon2;Parent=MsaT026730.1
Chr5	CDS	27560677	27560962	27560677	ID=cds.MsaT026730.1;Parent=MsaT026730.1
Chr5	exon	27561034	27561474	27561034	ID=MsaT026730.1.exon3;Parent=MsaT026730.1
Chr5	CDS	27561034	27561474	27561034	ID=cds.MsaT026730.1;Parent=MsaT026730.1

Gene Sequence

>MsaT026730.1

ATGAAGACAGACAGAGCAGCGCGAGATGCTAGGAGTGAAATAGAATTTGATGAACAAGAGGAACAAGAAGAGGAGATGGAGATTCCAGAAACACAAACACCAGTGCCAACGAGTTATGACTCACTTGGTAACTCAGCTTCACGTTCAAAACTCACCGTCGGAGTTGACGGAAGGAAAGGTAACGGCAACGGCGGTTTTGTTTCGACGGTGAGGTACCGAGAATGTCAGAAGAATCACGCCGTTAGCTTCGGTGGCCATGCTGTTGACGGTTGTTGCGAGTTTATCGCTGCCGGTGAAGAAGGAACACTTGAAGCTGTGCTTCCATCACCTCCACCGCCGCAATACCATCACCACAGCAATCAATTCTCGCCTTACTACCACCGTGCTCCTCCTTCCACAGCCGGTTACCTTCACCACCATCATCTTGCAACTCCGGTGGCACATCACAGACCGTTGGCTCTCCCTGCTGCTGCTTCCGGTGGTGGAATGAGCAGAGAAGATGATGATATGTCAAACCCTAGCAGTAGCGGTGGAGGAGGTGGTGGTGGTAGTGGTGGTGGTGGATCAAGGAAGAGATTCAGAACAAAATTCACTCAGGAACAGAAAGAGAAGTTGCTGGCTTTTGCTGAAGAACATGGTTGGAGAATTCAGAAACAAGATGAAGCTGCTATAGAACAGTTTTGTGCTGAGAATTGTATCAAGAGAAATGTTCTTAAAGTTTGGATGCACAATAACAAGAACACTCTTGGTAAGAAACCCTAA

Protein sequence

>MsaT026730.1

MKTDRAARDARSEIEFDEQEEQEEEMEIPETQTPVPTSYDSLGNSASRSKLTVGVDGRKGNGNGGFVSTVRYRECQKNHAVSFGGHAVDGCCEFIAAGEEGTLEAVLPSPPPPQYHHHSNQFSPYYHRAPPSTAGYLHHHHLATPVAHHRPLALPAAASGGGMSREDDDMSNPSSSGGGGGGGSGGGGSRKRFRTKFTQEQKEKLLAFAEEHGWRIQKQDEAAIEQFCAENCIKRNVLKVWMHNNKNTLGKKP*