AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026878

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026878 MtrunA17_Chr5g0417281 89.844 512 50 2 1 512 1 510 0.0 937
MsaG026878 MtrunA17_Chr5g0436761 53.592 515 176 8 1 510 1 457 4.06e-176 503
MsaG026878 MtrunA17_Chr5g0417211 76.972 317 67 3 1 313 1 315 1.43e-175 500
MsaG026878 MtrunA17_Chr5g0424931 48.991 545 211 10 1 510 8 520 1.17e-159 464
MsaG026878 MtrunA17_Chr2g0289221 70.794 315 87 4 1 312 9 321 1.37e-153 442
MsaG026878 MtrunA17_Chr2g0277461 45.825 515 197 11 4 510 5 445 1.62e-130 387
MsaG026878 MtrunA17_Chr3g0115271 40.422 569 262 14 2 513 9 557 1.84e-120 366
MsaG026878 MtrunA17_Chr1g0178011 50.411 365 164 9 1 354 1 359 1.95e-111 338
MsaG026878 MtrunA17_Chr1g0178011 50.000 78 39 0 426 503 359 436 1.00e-14 76.6
MsaG026878 MtrunA17_Chr5g0424741 39.888 534 239 13 1 469 1 517 2.96e-105 324
MsaG026878 MtrunA17_Chr5g0424801 49.425 348 147 10 1 332 1 335 5.09e-104 316
MsaG026878 MtrunA17_Chr5g0425261 49.713 348 146 10 1 332 1 335 3.43e-102 311
MsaG026878 MtrunA17_Chr4g0028971 37.317 477 212 11 1 415 1 452 1.03e-88 286
MsaG026878 MtrunA17_Chr2g0277531 45.367 313 157 9 6 312 14 318 1.87e-87 271
MsaG026878 MtrunA17_Chr2g0326761 40.201 398 174 9 9 351 8 396 1.90e-87 283
MsaG026878 MtrunA17_Chr1g0181211 39.518 415 180 9 2 353 16 422 5.70e-85 276
MsaG026878 MtrunA17_Chr1g0199681 40.102 394 179 8 9 351 91 478 2.39e-81 267
MsaG026878 MtrunA17_Chr7g0264171 32.740 562 269 16 9 495 28 555 1.20e-74 247
MsaG026878 MtrunA17_Chr5g0425661 52.062 194 89 2 12 204 37 227 2.60e-64 209
MsaG026878 MtrunA17_Chr7g0264161 39.935 308 160 8 22 312 2 301 2.71e-64 211
MsaG026878 MtrunA17_Chr2g0282961 34.493 345 192 8 12 332 53 387 4.33e-61 211
MsaG026878 MtrunA17_Chr4g0046371 34.659 352 186 9 12 332 38 376 1.87e-60 211
MsaG026878 MtrunA17_Chr4g0009751 35.988 339 191 8 12 332 19 349 4.77e-60 208
MsaG026878 MtrunA17_Chr2g0285461 34.545 385 215 12 9 368 20 392 2.62e-58 207
MsaG026878 MtrunA17_Chr8g0346711 36.901 355 190 10 2 332 14 358 4.70e-58 204
MsaG026878 MtrunA17_Chr2g0276781 35.965 342 192 7 12 334 23 356 2.62e-57 202
MsaG026878 MtrunA17_Chr5g0433301 33.773 379 218 11 12 367 24 392 1.00e-56 202
MsaG026878 MtrunA17_Chr7g0239061 33.807 352 207 8 1 334 3 346 1.39e-56 200
MsaG026878 MtrunA17_Chr8g0385791 34.530 362 211 8 12 354 59 413 1.24e-55 199
MsaG026878 MtrunA17_Chr1g0156321 35.211 355 194 11 12 342 43 385 4.12e-55 198
MsaG026878 MtrunA17_Chr8g0376771 33.069 378 229 10 9 368 20 391 8.57e-55 197
MsaG026878 MtrunA17_Chr3g0109391 33.509 379 219 11 12 367 26 394 9.19e-55 197
MsaG026878 MtrunA17_Chr2g0301251 34.091 352 204 7 9 340 20 363 4.15e-54 196
MsaG026878 MtrunA17_Chr2g0326281 34.211 380 205 14 12 352 56 429 5.91e-53 192
MsaG026878 MtrunA17_Chr4g0070181 33.429 350 213 6 9 342 20 365 7.01e-53 193
MsaG026878 MtrunA17_Chr8g0389081 34.085 355 192 10 9 335 29 369 2.35e-52 191
MsaG026878 MtrunA17_Chr4g0029671 34.857 350 192 12 12 332 54 396 1.85e-50 184
MsaG026878 MtrunA17_Chr5g0425651 51.240 121 52 5 230 344 1 120 1.20e-27 107
MsaG026878 MtrunA17_Chr5g0424791 43.919 148 73 4 63 210 14 151 2.20e-27 107
MsaG026878 MtrunA17_Chr8g0356211 26.549 226 150 4 122 335 2 223 1.46e-23 101
MsaG026878 MtrunA17_Chr5g0419861 42.478 113 58 3 53 162 15 123 3.12e-19 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026878 AT1G77850.1 36.194 536 274 11 8 494 15 531 7.90e-106 328
MsaG026878 AT1G77850.2 36.262 535 273 11 9 494 16 531 1.15e-105 328
MsaG026878 AT2G28350.2 40.887 406 176 9 4 351 2 401 3.29e-87 282
MsaG026878 AT2G28350.1 40.887 406 176 9 4 351 2 401 4.23e-87 282
MsaG026878 AT4G30080.1 36.344 454 219 8 9 411 16 450 2.60e-85 277
MsaG026878 AT4G23980.1 37.798 336 189 8 12 332 12 342 1.48e-63 219
MsaG026878 AT4G23980.2 37.798 336 189 8 12 332 12 342 1.55e-63 218
MsaG026878 AT2G33860.1 35.977 353 186 8 12 337 54 393 6.36e-63 216
MsaG026878 AT5G37020.2 33.421 380 216 10 12 366 22 389 1.83e-59 210
MsaG026878 AT5G37020.1 33.421 380 216 10 12 366 22 389 2.53e-59 210
MsaG026878 AT2G46530.1 36.088 363 200 11 12 355 21 370 1.29e-58 204
MsaG026878 AT2G46530.3 36.088 363 200 11 12 355 42 391 1.89e-58 204
MsaG026878 AT3G61830.1 35.608 337 194 9 12 332 25 354 3.55e-58 203
MsaG026878 AT1G35520.1 36.486 370 201 11 12 361 27 382 4.01e-57 200
MsaG026878 AT5G60450.2 35.519 366 210 10 7 349 36 398 1.37e-56 202
MsaG026878 AT1G19850.2 34.595 370 201 11 9 347 40 399 2.07e-56 202
MsaG026878 AT1G19850.1 34.595 370 201 11 9 347 51 410 2.98e-56 202
MsaG026878 AT1G30330.1 34.711 363 204 11 12 351 23 375 3.04e-56 202
MsaG026878 AT1G30330.3 34.711 363 204 11 12 351 25 377 3.07e-56 202
MsaG026878 AT1G30330.2 34.711 363 204 11 12 351 25 377 3.07e-56 202
MsaG026878 AT5G60450.1 35.734 361 206 10 12 349 65 422 3.43e-56 201
MsaG026878 AT1G34410.1 36.950 341 180 10 12 332 27 352 5.58e-56 197
MsaG026878 AT1G59750.4 34.985 343 194 8 12 334 22 355 3.73e-55 196
MsaG026878 AT1G59750.2 34.503 342 197 7 12 334 22 355 4.21e-55 196
MsaG026878 AT5G62000.2 33.880 366 208 9 12 354 61 415 4.51e-55 198
MsaG026878 AT5G62000.5 33.880 366 208 9 12 354 61 415 4.51e-55 198
MsaG026878 AT5G62000.3 33.880 366 208 9 12 354 61 415 4.51e-55 198
MsaG026878 AT5G62000.1 33.880 366 208 9 12 354 61 415 4.51e-55 198
MsaG026878 AT1G59750.1 34.503 342 197 7 12 334 22 355 4.61e-55 196
MsaG026878 AT1G59750.3 34.503 342 197 7 12 334 22 355 4.61e-55 196
MsaG026878 AT5G62000.4 33.880 366 208 9 12 354 61 415 4.81e-55 198
MsaG026878 AT1G35540.1 36.337 344 184 10 12 335 27 355 9.81e-55 194
MsaG026878 AT1G35240.1 36.070 341 183 10 12 332 27 352 2.13e-54 193
MsaG026878 AT1G34390.1 35.135 370 204 12 12 361 27 380 8.99e-54 191
MsaG026878 AT1G34310.1 35.638 376 206 12 7 361 22 382 1.02e-53 191
MsaG026878 AT1G19220.1 33.333 354 208 8 9 342 20 365 5.79e-52 191
MsaG026878 AT5G20730.3 32.591 359 205 7 9 342 21 367 4.88e-50 185
MsaG026878 AT5G20730.1 32.591 359 205 7 9 342 21 367 5.12e-50 185
MsaG026878 AT5G20730.2 32.869 359 203 8 9 342 21 366 1.45e-49 184
MsaG026878 AT2G33860.2 38.655 238 129 4 114 337 2 236 4.58e-49 175
MsaG026878 AT3G61830.2 36.014 286 165 7 59 332 24 303 6.90e-47 171
MsaG026878 AT1G34170.1 31.287 342 200 9 12 332 25 352 3.08e-46 169
MsaG026878 AT5G37020.3 34.317 271 153 7 114 366 31 294 1.72e-43 164
MsaG026878 AT2G46530.2 37.597 258 140 8 111 355 34 283 4.59e-41 155
MsaG026878 AT1G34170.2 30.952 336 183 11 12 332 25 326 2.16e-38 147
MsaG026878 AT1G34170.3 31.065 338 184 11 12 334 25 328 4.26e-38 147
MsaG026878 AT1G43950.1 36.364 209 113 5 12 213 27 222 3.62e-34 129
MsaG026878 ATMG00940.1 39.252 107 59 3 202 308 44 144 7.06e-16 75.9
MsaG026878 AT4G01500.1 29.286 140 92 3 114 251 36 170 3.13e-11 65.1

Find 133 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTTAAACTCTATATT+TGG 0.091140 5:-30702887 None:intergenic
CCCGGTTCATTTATGCTATT+TGG 0.108058 5:+30703199 MsaT026878.1:CDS
TCTCAATATCATAGGTTAAA+TGG 0.141063 5:+30702669 MsaT026878.1:intron
GCACAGCACTGCCAGATTTC+TGG 0.173902 5:-30701209 None:intergenic
TTCTGCATTCAGTGATAAAA+AGG 0.199270 5:+30702820 MsaT026878.1:CDS
GATAACGGACAAATCGTTTA+TGG 0.245740 5:+30701847 MsaT026878.1:CDS
CACTTCTGGTCCTATATCTT+TGG 0.246322 5:+30703090 MsaT026878.1:CDS
CATGTTTACTACTTCCCATT+AGG 0.255048 5:+30701266 MsaT026878.1:CDS
GGGAGAGTGTGTTAGGGTTT+GGG 0.258688 5:-30701313 None:intergenic
TGAACCGGGACTAACTTTCT+TGG 0.329918 5:-30703185 None:intergenic
TCAAGAAGGGAGAGTGTGTT+AGG 0.330307 5:-30701320 None:intergenic
TCTGGAACCTGCGAGACTTT+AGG 0.330762 5:-30702698 None:intergenic
TAAACATGAGAATATAGTTT+TGG 0.333605 5:-30701251 None:intergenic
GATAAAAGCAATGGTTTCCT+TGG 0.335382 5:+30703022 MsaT026878.1:CDS
GTGACATTCACGGTAAAGTT+TGG 0.340609 5:+30701648 MsaT026878.1:CDS
TGAGTCTTTCATGAAAATTA+AGG 0.342069 5:-30701768 None:intergenic
GATTTCTGGCTCAACACGGC+TGG 0.344702 5:-30701195 None:intergenic
AGGGAGAGTGTGTTAGGGTT+TGG 0.345088 5:-30701314 None:intergenic
ATTGTTCAACAATGTTTGAT+TGG 0.354882 5:-30702924 None:intergenic
TTTCAAGAATTAGTTATTAA+GGG 0.358073 5:+30703250 MsaT026878.1:CDS
TCAAGTGAGCCACACACTTC+TGG 0.358411 5:+30703076 MsaT026878.1:CDS
ACCAAATAGCATAAATGAAC+CGG 0.359465 5:-30703200 None:intergenic
ATGTTTACTACTTCCCATTA+GGG 0.360944 5:+30701267 MsaT026878.1:CDS
ATGTGTTCTATGTGCCCTAA+TGG 0.363040 5:-30701281 None:intergenic
ATGAATATTAAGTGGAATCT+TGG 0.367936 5:+30701977 MsaT026878.1:CDS
TTTCCTTGGGAACAATTCTA+TGG 0.382248 5:+30703036 MsaT026878.1:CDS
CTCAATATCATAGGTTAAAT+GGG 0.383997 5:+30702670 MsaT026878.1:intron
GTTTATGGAGACAGTGGAAT+TGG 0.387256 5:+30701862 MsaT026878.1:CDS
GCCGTGTTGAGCCAGAAATC+TGG 0.395763 5:+30701198 MsaT026878.1:CDS
GAAATCTGGCAGTGCTGTGC+AGG 0.404779 5:+30701212 MsaT026878.1:CDS
AATGTTTAAACTCTATATTT+GGG 0.405482 5:-30702886 None:intergenic
AAAGAGATGCTCAAAATTTG+AGG 0.405775 5:+30702039 MsaT026878.1:CDS
GTTCGCCGACAGACAAAGTT+TGG 0.410958 5:+30701812 MsaT026878.1:CDS
AGCATCCGCCATGGACGATT+AGG 0.415227 5:-30702085 None:intergenic
CATGATATTTCTAACTCTCT+TGG 0.416099 5:+30703130 MsaT026878.1:CDS
TTCCAGAGCGAGTCAGTCCT+TGG 0.420269 5:+30702714 MsaT026878.1:CDS
CATTGCATCCTCCACAACTT+TGG 0.422061 5:-30701957 None:intergenic
GACATCTTCTTACCACTGAT+TGG 0.435417 5:+30701705 MsaT026878.1:CDS
ATGATATTTCTAACTCTCTT+GGG 0.435417 5:+30703131 MsaT026878.1:CDS
TCTTGGTCTTCACGAACCTT+AGG 0.438896 5:-30701473 None:intergenic
TCTCTTGGGACAAAGTTTGC+CGG 0.439539 5:+30703145 MsaT026878.1:CDS
TCTTGAAAATCGCTTCCAAC+AGG 0.440910 5:-30703235 None:intergenic
TTGATTGCATGCAGGGAGCC+AGG 0.442279 5:+30702957 MsaT026878.1:CDS
ATTAATTTCGCGCATTCCAC+TGG 0.445870 5:-30701572 None:intergenic
AGACCAAGATGATGGCAAAA+AGG 0.450916 5:+30701487 MsaT026878.1:CDS
TCCCAAGGACTGACTCGCTC+TGG 0.456814 5:-30702716 None:intergenic
GGAGAGTGTGTTAGGGTTTG+GGG 0.461223 5:-30701312 None:intergenic
GCTCTTCTTGATTGCATGCA+GGG 0.464239 5:+30702950 MsaT026878.1:CDS
CCGCCATTGTTAGCATCTGA+TGG 0.465747 5:-30701536 None:intergenic
GTGTATTATCCAACGGCTAA+AGG 0.466705 5:+30701911 MsaT026878.1:CDS
CGCCATTGTTAGCATCTGAT+GGG 0.467986 5:-30701535 None:intergenic
ATAAAAGCAATGGTTTCCTT+GGG 0.470549 5:+30703023 MsaT026878.1:CDS
GAGACTGTGCAGAGAATGAA+TGG 0.471564 5:-30701353 None:intergenic
GGTGACAAGTCCAAAGATAT+AGG 0.472899 5:-30703100 None:intergenic
TCCAGAGCGAGTCAGTCCTT+GGG 0.477866 5:+30702715 MsaT026878.1:CDS
TGCTCTTCTTGATTGCATGC+AGG 0.478114 5:+30702949 MsaT026878.1:CDS
AAAGCTTAGAAAATCTGATC+AGG 0.481737 5:+30702793 MsaT026878.1:CDS
ATGATGATTGTAATTTGTAA+AGG 0.488803 5:+30703275 MsaT026878.1:CDS
CTCCTTCGTCCCAAATATAG+AGG 0.491263 5:+30702847 MsaT026878.1:CDS
AGTGTGTTAGGGTTTGGGGA+TGG 0.498039 5:-30701308 None:intergenic
TGAACAGCGTTGTTAGTGAC+AGG 0.502582 5:-30701437 None:intergenic
TGGGGTGAGAGTCTTGGTAT+AGG 0.502872 5:-30701516 None:intergenic
CAAGAGAGTTAGAAATATCA+TGG 0.505101 5:-30703129 None:intergenic
ATTTCTGGCTCAACACGGCT+GGG 0.505787 5:-30701194 None:intergenic
TCACGAACCTTAGGAGATTG+AGG 0.514142 5:-30701464 None:intergenic
TTACACCAACCTTTAGCCGT+TGG 0.523651 5:-30701920 None:intergenic
GGTAAGATCATAAAACCTGT+TGG 0.525681 5:+30703220 MsaT026878.1:CDS
ATCTGATGGGGTGAGAGTCT+TGG 0.526809 5:-30701522 None:intergenic
TTTGTGAATGATAAAAGCAA+TGG 0.529057 5:+30703013 MsaT026878.1:CDS
TGTGTTCTATGTGCCCTAAT+GGG 0.529576 5:-30701280 None:intergenic
GGTGTTGCTGTTGTCAGTAC+CGG 0.533496 5:-30703164 None:intergenic
AATATTAAGTGGAATCTTGG+AGG 0.534264 5:+30701980 MsaT026878.1:CDS
ATCTCCATCAGTGACATTCA+CGG 0.537750 5:+30701638 MsaT026878.1:CDS
GTTGACAAGAAGAAACTAGT+TGG 0.539036 5:+30701737 MsaT026878.1:CDS
AAGAGATGCTCAAAATTTGA+GGG 0.539733 5:+30702040 MsaT026878.1:CDS
GTTCAACAATGTTTGATTGG+AGG 0.545186 5:-30702921 None:intergenic
TCGTCCATGGCGGATGCTTG+AGG 0.546119 5:+30702090 MsaT026878.1:CDS
TGAAGTTGTGTATTATCCAA+CGG 0.547938 5:+30701904 MsaT026878.1:CDS
TGGATACAGTGCAAATATGT+CGG 0.548922 5:-30702753 None:intergenic
AGGATGCAATGAATATTAAG+TGG 0.550257 5:+30701969 MsaT026878.1:CDS
CAAGAAGGGAGAGTGTGTTA+GGG 0.554864 5:-30701319 None:intergenic
TGGTGCTGCTGCTAAGATAA+CGG 0.563545 5:+30701832 MsaT026878.1:CDS
CTGATGGAGATTTCTTGAGA+CGG 0.566848 5:-30701626 None:intergenic
CGGTGCATTCTCCGTGCCAG+TGG 0.572523 5:+30701556 MsaT026878.1:CDS
ATGGACGGGAACGGTCAAGA+AGG 0.574895 5:-30701334 None:intergenic
AGCCTCTATATTTGGGACGA+AGG 0.575856 5:-30702849 None:intergenic
TCACGAACCTCAATCTCCTA+AGG 0.575969 5:+30701457 MsaT026878.1:CDS
GCTGGGTTGCTGAGAAGACA+TGG 0.576060 5:-30701177 None:intergenic
CTGTGCAGAGAATGAATGGA+CGG 0.581540 5:-30701349 None:intergenic
TATCTGCTCCTAATCGTCCA+TGG 0.582308 5:+30702077 MsaT026878.1:CDS
GTTCGTGAAGACCAAGATGA+TGG 0.585038 5:+30701479 MsaT026878.1:CDS
TAGTTTGACGAAGACTTCAT+CGG 0.586807 5:-30701405 None:intergenic
CGAAGACTTCATCGGTGTGA+AGG 0.586907 5:-30701397 None:intergenic
AAAGTTGGAAGAAGTTGATG+TGG 0.587804 5:-30702993 None:intergenic
GGATGAACCTAAAGTCTCGC+AGG 0.588394 5:+30702691 MsaT026878.1:CDS
AAGTTTCGCCACGTCTACCG+TGG 0.588988 5:+30701671 MsaT026878.1:CDS
TTAGAAATATCATGGCTATG+AGG 0.592683 5:-30703121 None:intergenic
TGGACGGGAACGGTCAAGAA+GGG 0.593041 5:-30701333 None:intergenic
CCAAATAGCATAAATGAACC+GGG 0.595203 5:-30703199 None:intergenic
TCAGATGGAAATATCTCAGT+CGG 0.595347 5:+30701788 MsaT026878.1:CDS
TGTCGTTGATGCCAAAGTTG+TGG 0.596764 5:+30701946 MsaT026878.1:CDS
CAGTATTGAGGTCAAGCGGT+GGG 0.600081 5:-30701598 None:intergenic
CAGCACCAAACTTTGTCTGT+CGG 0.605931 5:-30701817 None:intergenic
ATTATCCAACGGCTAAAGGT+TGG 0.606477 5:+30701915 MsaT026878.1:CDS
TTTACCGTGAATGTCACTGA+TGG 0.607693 5:-30701642 None:intergenic
GAAATAGTTTCAACCTCCCA+AGG 0.609478 5:-30702731 None:intergenic
AACACCAAGAAAGTTAGTCC+CGG 0.610950 5:+30703181 MsaT026878.1:CDS
ATATTAAGTGGAATCTTGGA+GGG 0.616357 5:+30701981 MsaT026878.1:CDS
TGTGCAGAGAATGAATGGAC+GGG 0.620486 5:-30701348 None:intergenic
AGAGAATGAATGGACGGGAA+CGG 0.620696 5:-30701343 None:intergenic
CTGCCATAGAATTGTTCCCA+AGG 0.622061 5:-30703039 None:intergenic
AGTGGTAAGAAGATGTCGCA+AGG 0.625494 5:-30701699 None:intergenic
AGGGAGTCCCACGGTAGACG+TGG 0.627277 5:-30701679 None:intergenic
AGAGCGAGTCAGTCCTTGGG+AGG 0.629409 5:+30702718 MsaT026878.1:CDS
CGTTGTTAGTGACAGGAGTG+AGG 0.629524 5:-30701430 None:intergenic
GACAAGAAGAAACTAGTTGG+TGG 0.630685 5:+30701740 MsaT026878.1:CDS
TATACCTCAAGCATCCGCCA+TGG 0.633522 5:-30702094 None:intergenic
AACGAACTCACTCCAATCAG+TGG 0.642790 5:-30701717 None:intergenic
CATTCTCTGCACAGTCTCCG+CGG 0.651521 5:+30701358 MsaT026878.1:CDS
AGTTTCGCCACGTCTACCGT+GGG 0.652406 5:+30701672 MsaT026878.1:CDS
CGTTGATGCCAAAGTTGTGG+AGG 0.653519 5:+30701949 MsaT026878.1:CDS
CAAATCGTTTATGGAGACAG+TGG 0.655278 5:+30701856 MsaT026878.1:CDS
TTTCGCGCATTCCACTGGCA+CGG 0.656401 5:-30701567 None:intergenic
ACCCCATCAGATGCTAACAA+TGG 0.657432 5:+30701533 MsaT026878.1:CDS
GTGGTAAGAAGATGTCGCAA+GGG 0.664196 5:-30701698 None:intergenic
GCCATTGTTAGCATCTGATG+GGG 0.677657 5:-30701534 None:intergenic
TCAGTATTGAGGTCAAGCGG+TGG 0.680037 5:-30701599 None:intergenic
GCCAGATTTCTGGCTCAACA+CGG 0.683376 5:-30701199 None:intergenic
GATATAGGACCAGAAGTGTG+TGG 0.690755 5:-30703085 None:intergenic
CCATCAGATGCTAACAATGG+CGG 0.695708 5:+30701536 MsaT026878.1:CDS
GATGTCGCAAGGGAGTCCCA+CGG 0.712482 5:-30701688 None:intergenic
CTGCTCCTAATCGTCCATGG+CGG 0.723801 5:+30702080 MsaT026878.1:CDS
GTCAGCGAGTAAATCAACCG+CGG 0.752817 5:-30701375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 30701179 30703294 30701179 ID=MsaG026878
Chr5 mRNA 30701179 30703294 30701179 ID=MsaT026878.1;Parent=MsaG026878
Chr5 exon 30701179 30702111 30701179 ID=MsaT026878.1.exon1;Parent=MsaT026878.1
Chr5 CDS 30701179 30702111 30701179 ID=cds.MsaT026878.1;Parent=MsaT026878.1
Chr5 exon 30702683 30703294 30702683 ID=MsaT026878.1.exon2;Parent=MsaT026878.1
Chr5 CDS 30702683 30703294 30702683 ID=cds.MsaT026878.1;Parent=MsaT026878.1
Gene Sequence

>MsaT026878.1

ATGTCTTCTCAGCAACCCAGCCGTGTTGAGCCAGAAATCTGGCAGTGCTGTGCAGGCAGTTCCGTTAAAATTCCAAAACTATATTCTCATGTTTACTACTTCCCATTAGGGCACATAGAACACATATGTCCATCCCCAAACCCTAACACACTCTCCCTTCTTGACCGTTCCCGTCCATTCATTCTCTGCACAGTCTCCGCGGTTGATTTACTCGCTGACCTTCACACCGATGAAGTCTTCGTCAAACTACTCCTCACTCCTGTCACTAACAACGCTGTTCACGAACCTCAATCTCCTAAGGTTCGTGAAGACCAAGATGATGGCAAAAAGGTTGTTTCCTATACCAAGACTCTCACCCCATCAGATGCTAACAATGGCGGTGCATTCTCCGTGCCAGTGGAATGCGCGAAATTAATCTTCCCACCGCTTGACCTCAATACTGAAAAGCCGTCTCAAGAAATCTCCATCAGTGACATTCACGGTAAAGTTTGGAAGTTTCGCCACGTCTACCGTGGGACTCCCTTGCGACATCTTCTTACCACTGATTGGAGTGAGTTCGTTGACAAGAAGAAACTAGTTGGTGGCGATTCCTTAATTTTCATGAAAGACTCAGATGGAAATATCTCAGTCGGAGTTCGCCGACAGACAAAGTTTGGTGCTGCTGCTAAGATAACGGACAAATCGTTTATGGAGACAGTGGAATTGGCAGATAAAAACATGGCTTTTGAAGTTGTGTATTATCCAACGGCTAAAGGTTGGTGTAATTTTGTCGTTGATGCCAAAGTTGTGGAGGATGCAATGAATATTAAGTGGAATCTTGGAGGGAGAATTGAACTTTCTTTGAAGAATTACGATTCTTCAAAGAGATGCTCAAAATTTGAGGGAACAATATCTGCTCTATCTGCTCCTAATCGTCCATGGCGGATGCTTGAGGTTAAATGGGATGAACCTAAAGTCTCGCAGGTTCCAGAGCGAGTCAGTCCTTGGGAGGTTGAAACTATTTCCGACATATTTGCACTGTATCCACAATTCCACCCGACAAAAAAGCTTAGAAAATCTGATCAGGAATCTTCTGCATTCAGTGATAAAAAGGGAGACTCCTTCGTCCCAAATATAGAGGCTTTTTTGAAGATGGTCCCAAATATAGAGTTTAAACATTTCGATATGACATCCTCCAATCAAACATTGTTGAACAATGATGCTCTTCTTGATTGCATGCAGGGAGCCAGGCACCGTCTTTTTTCCACATCAACTTCTTCCAACTTTGTGAATGATAAAAGCAATGGTTTCCTTGGGAACAATTCTATGGCAGTGTTGAGTGATGTGTCAAGTGAGCCACACACTTCTGGTCCTATATCTTTGGACTTGTCACCTCATAGCCATGATATTTCTAACTCTCTTGGGACAAAGTTTGCCGGTACTGACAACAGCAACACCAAGAAAGTTAGTCCCGGTTCATTTATGCTATTTGGTAAGATCATAAAACCTGTTGGAAGCGATTTTCAAGAATTAGTTATTAAGGGAAATGATGATTGTAATTTGTAA
Protein sequence

>MsaT026878.1

MSSQQPSRVEPEIWQCCAGSSVKIPKLYSHVYYFPLGHIEHICPSPNPNTLSLLDRSRPFILCTVSAVDLLADLHTDEVFVKLLLTPVTNNAVHEPQSPKVREDQDDGKKVVSYTKTLTPSDANNGGAFSVPVECAKLIFPPLDLNTEKPSQEISISDIHGKVWKFRHVYRGTPLRHLLTTDWSEFVDKKKLVGGDSLIFMKDSDGNISVGVRRQTKFGAAAKITDKSFMETVELADKNMAFEVVYYPTAKGWCNFVVDAKVVEDAMNIKWNLGGRIELSLKNYDSSKRCSKFEGTISALSAPNRPWRMLEVKWDEPKVSQVPERVSPWEVETISDIFALYPQFHPTKKLRKSDQESSAFSDKKGDSFVPNIEAFLKMVPNIEFKHFDMTSSNQTLLNNDALLDCMQGARHRLFSTSTSSNFVNDKSNGFLGNNSMAVLSDVSSEPHTSGPISLDLSPHSHDISNSLGTKFAGTDNSNTKKVSPGSFMLFGKIIKPVGSDFQELVIKGNDDCNL*