Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026916 | XP_013448301.1 | 85.042 | 361 | 39 | 2 | 1 | 361 | 1 | 346 | 0.0 | 624 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026916 | sp|Q8RWD7|EMB74_ARATH | 38.938 | 113 | 62 | 3 | 22 | 132 | 64 | 171 | 1.79e-16 | 82.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026916 | tr|A0A072TZN4|A0A072TZN4_MEDTR | 85.042 | 361 | 39 | 2 | 1 | 361 | 1 | 346 | 0.0 | 624 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG026916 | TR | Others |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000461 | MsaG026916 | 0.805829 | 1.870237e-49 | 2.890747e-47 |
| MsaG011845 | MsaG026916 | 0.813767 | 3.736927e-51 | 7.003405e-49 |
| MsaG026916 | MsaG036696 | 0.800645 | 2.188409e-48 | 3.002328e-46 |
| MsaG026916 | MsaG041153 | 0.810147 | 2.277391e-50 | 3.903038e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026916 | MtrunA17_Chr7g0231611 | 85.042 | 361 | 39 | 2 | 1 | 361 | 1 | 346 | 0.0 | 624 |
| MsaG026916 | MtrunA17_Chr5g0416851 | 85.319 | 361 | 38 | 2 | 1 | 361 | 1 | 346 | 0.0 | 607 |
| MsaG026916 | MtrunA17_Chr2g0282701 | 73.308 | 266 | 41 | 3 | 96 | 361 | 42 | 277 | 4.03e-125 | 360 |
| MsaG026916 | MtrunA17_Chr1g0182611 | 32.955 | 176 | 94 | 8 | 1 | 164 | 1 | 164 | 1.10e-18 | 87.0 |
| MsaG026916 | MtrunA17_Chr1g0182621 | 37.815 | 119 | 60 | 4 | 22 | 134 | 28 | 138 | 1.52e-16 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG026916 | AT1G58210.1 | 38.938 | 113 | 62 | 3 | 22 | 132 | 64 | 171 | 1.82e-17 | 82.0 |
| MsaG026916 | AT5G02520.2 | 35.294 | 102 | 58 | 3 | 15 | 110 | 11 | 110 | 1.07e-12 | 69.7 |
| MsaG026916 | AT5G02520.1 | 35.294 | 102 | 58 | 3 | 15 | 110 | 11 | 110 | 1.13e-12 | 69.7 |
| MsaG026916 | AT5G02520.3 | 35.294 | 102 | 58 | 3 | 15 | 110 | 11 | 110 | 1.34e-12 | 69.3 |
Find 72 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCCTCTACAATCTTCT+TGG | 0.224995 | 5:+31300890 | None:intergenic |
| TTATAAATCACGCCAATATT+TGG | 0.228451 | 5:+31301290 | None:intergenic |
| CCTTGCGGTCACTTGATAAT+TGG | 0.242121 | 5:+31300925 | None:intergenic |
| TCCACTTTACGCAAGGAAAT+TGG | 0.248343 | 5:-31300955 | MsaT026916.1:CDS |
| GTGTAGTGGTGGATCGTATT+GGG | 0.274886 | 5:+31302842 | None:intergenic |
| ATCCTCTACAATCTTCTTCT+TGG | 0.277613 | 5:+31300860 | None:intergenic |
| GTGATTTATAATGGTGAAAA+TGG | 0.304153 | 5:-31301278 | MsaT026916.1:CDS |
| CGGTTGGATTTCCACACAAT+TGG | 0.311637 | 5:-31301472 | MsaT026916.1:CDS |
| AAGTCACATGATGCTTAAAA+AGG | 0.317507 | 5:-31300787 | MsaT026916.1:CDS |
| AATATTGGCGTGATTTATAA+TGG | 0.332979 | 5:-31301287 | MsaT026916.1:CDS |
| AGCTTGTCTCAACCAAATAT+TGG | 0.339420 | 5:-31301302 | MsaT026916.1:CDS |
| GGTGTAGTGGTGGATCGTAT+TGG | 0.352995 | 5:+31302841 | None:intergenic |
| GCCTCACGTACGTCTCAAAA+TGG | 0.354055 | 5:-31302428 | MsaT026916.1:CDS |
| TCCAATTTCCTTGCGTAAAG+TGG | 0.363457 | 5:+31300954 | None:intergenic |
| TGCAAGGGTTTAGCTCTTGC+TGG | 0.372413 | 5:-31302654 | MsaT026916.1:CDS |
| AATTATTCAGCTCATAGTTC+AGG | 0.376079 | 5:-31301446 | MsaT026916.1:CDS |
| GCTAAACCCTTGCATTGGTT+TGG | 0.379957 | 5:+31302663 | None:intergenic |
| AGCAGCTGATGAAACTTTGC+AGG | 0.381501 | 5:-31301213 | MsaT026916.1:CDS |
| TGAAACTTTGCAGGAAGATA+TGG | 0.392140 | 5:-31301204 | MsaT026916.1:CDS |
| CAGATTGACGTGATTTCTAA+TGG | 0.393027 | 5:-31301077 | MsaT026916.1:CDS |
| GAAAACGTGGATAAAGTTAC+TGG | 0.399696 | 5:-31301413 | MsaT026916.1:CDS |
| TCTTGCTGGCATTGCTTCAA+TGG | 0.402134 | 5:-31302640 | MsaT026916.1:CDS |
| GCAATGTTGTCATTGCCTTC+CGG | 0.413401 | 5:+31301122 | None:intergenic |
| GGTGGATCGTATTGGGGTCT+TGG | 0.417450 | 5:+31302849 | None:intergenic |
| GGGTCTTAAGTTGTCTCAAC+AGG | 0.433791 | 5:-31301156 | MsaT026916.1:CDS |
| AAGAAGATTGTAGAGGATAC+TGG | 0.442437 | 5:-31300855 | MsaT026916.1:CDS |
| AAACCCTTGCATTGGTTTGG+AGG | 0.442882 | 5:+31302666 | None:intergenic |
| AGCCTGGTGGATCTTCTAAC+AGG | 0.444598 | 5:+31300732 | None:intergenic |
| TTTAGATTTGAAGACTGAGT+TGG | 0.447708 | 5:+31302813 | None:intergenic |
| CTGGTAACTTGAGTAGTAGT+AGG | 0.448365 | 5:-31300836 | MsaT026916.1:CDS |
| ATCTTGAGTCTCTACAACAT+TGG | 0.448912 | 5:+31302484 | None:intergenic |
| GCAATGTTGTCATTACCTTC+AGG | 0.449864 | 5:+31301179 | None:intergenic |
| GAAGGTAATGACAACATTGC+GGG | 0.450334 | 5:-31301176 | MsaT026916.1:CDS |
| ACAGGTATGTCGCCATTTCT+CGG | 0.460769 | 5:-31301492 | MsaT026916.1:intron |
| TCTTGAGTCTCTACAACATT+GGG | 0.460912 | 5:+31302485 | None:intergenic |
| TTTGAAGACTGAGTTGGAGA+GGG | 0.464825 | 5:+31302819 | None:intergenic |
| GTGGAAATCCAACCGAGAAA+TGG | 0.476795 | 5:+31301480 | None:intergenic |
| GTTGTCTCAACAGGAAGGTA+TGG | 0.491152 | 5:-31301147 | MsaT026916.1:CDS |
| TCCATGAGCCAGGTAGTGTT+AGG | 0.493476 | 5:-31300980 | MsaT026916.1:CDS |
| GTATGTCGCCATTTCTCGGT+TGG | 0.501495 | 5:-31301488 | MsaT026916.1:CDS |
| ACCTAACACTACCTGGCTCA+TGG | 0.518389 | 5:+31300979 | None:intergenic |
| TGAGTTGGAGAGGGGTGTAG+TGG | 0.518631 | 5:+31302828 | None:intergenic |
| TTGAAGACTGAGTTGGAGAG+GGG | 0.520107 | 5:+31302820 | None:intergenic |
| CAAGAGCTAAACCCTTGCAT+TGG | 0.528805 | 5:+31302658 | None:intergenic |
| TTTGATCCTATCCATGAGCC+AGG | 0.537168 | 5:-31300990 | MsaT026916.1:CDS |
| GTTGGAGAGGGGTGTAGTGG+TGG | 0.540155 | 5:+31302831 | None:intergenic |
| TCAACAGGAAGGTATGGATC+CGG | 0.549502 | 5:-31301141 | MsaT026916.1:CDS |
| ATTTGAAGACTGAGTTGGAG+AGG | 0.552156 | 5:+31302818 | None:intergenic |
| ACACTACCTGGCTCATGGAT+AGG | 0.552798 | 5:+31300984 | None:intergenic |
| TGGTAACTTGAGTAGTAGTA+GGG | 0.576047 | 5:-31300835 | MsaT026916.1:CDS |
| AACAGTTGACGAGACTTCAC+AGG | 0.579898 | 5:-31301357 | MsaT026916.1:CDS |
| TGTAGTGGTGGATCGTATTG+GGG | 0.583890 | 5:+31302843 | None:intergenic |
| TTTATAATTTAGTAGCCTGG+TGG | 0.591703 | 5:+31300719 | None:intergenic |
| TTAGGTCTCCACTTTACGCA+AGG | 0.595503 | 5:-31300962 | MsaT026916.1:CDS |
| GAAACCTCCAAACCAATGCA+AGG | 0.605866 | 5:-31302670 | MsaT026916.1:CDS |
| AGTGGAGACCTAACACTACC+TGG | 0.609938 | 5:+31300972 | None:intergenic |
| TGGTACGTTTCTTATCCTTG+CGG | 0.610799 | 5:+31300910 | None:intergenic |
| GGCATCACTGTAATGTTCCG+CGG | 0.614466 | 5:-31302461 | MsaT026916.1:CDS |
| CCAATTATCAAGTGACCGCA+AGG | 0.618108 | 5:-31300925 | MsaT026916.1:CDS |
| CTCCTGTTAGAAGATCCACC+AGG | 0.618280 | 5:-31300734 | MsaT026916.1:CDS |
| CAGGAAGATATGGAACCTGA+AGG | 0.622434 | 5:-31301194 | MsaT026916.1:CDS |
| CTTAAGTTGTCTCAACAGGA+AGG | 0.626667 | 5:-31301152 | MsaT026916.1:CDS |
| AAACCTCCAAACCAATGCAA+GGG | 0.637222 | 5:-31302669 | MsaT026916.1:CDS |
| TTAGAAATCACGTCAATCTG+TGG | 0.650770 | 5:+31301080 | None:intergenic |
| AGGAAATGAATGTGAAAACG+TGG | 0.652661 | 5:-31301426 | MsaT026916.1:CDS |
| GAGACTTCACAGGAAGCTGA+AGG | 0.656001 | 5:-31301347 | MsaT026916.1:CDS |
| AATGTTGTAGAGACTCAAGA+TGG | 0.656225 | 5:-31302482 | MsaT026916.1:CDS |
| ACGTACCAAGAAGATTGTAG+AGG | 0.670592 | 5:-31300895 | MsaT026916.1:CDS |
| TACCAAGAAGAAGATTGTAG+AGG | 0.672640 | 5:-31300862 | MsaT026916.1:CDS |
| TGAAGGTAATGACAACATTG+CGG | 0.680073 | 5:-31301177 | MsaT026916.1:CDS |
| CAGGAAGGTATGGATCCGGA+AGG | 0.684185 | 5:-31301137 | MsaT026916.1:CDS |
| GTGAGGCGTTAATCAAACCG+CGG | 0.770302 | 5:+31302444 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 31300713 | 31302878 | 31300713 | ID=MsaG026916 |
| Chr5 | mRNA | 31300713 | 31302878 | 31300713 | ID=MsaT026916.1;Parent=MsaG026916 |
| Chr5 | exon | 31300713 | 31301510 | 31300713 | ID=MsaT026916.1.exon4;Parent=MsaT026916.1 |
| Chr5 | CDS | 31300713 | 31301510 | 31300713 | ID=cds.MsaT026916.1;Parent=MsaT026916.1 |
| Chr5 | exon | 31302415 | 31302553 | 31302415 | ID=MsaT026916.1.exon3;Parent=MsaT026916.1 |
| Chr5 | CDS | 31302415 | 31302553 | 31302415 | ID=cds.MsaT026916.1;Parent=MsaT026916.1 |
| Chr5 | exon | 31302639 | 31302718 | 31302639 | ID=MsaT026916.1.exon2;Parent=MsaT026916.1 |
| Chr5 | CDS | 31302639 | 31302718 | 31302639 | ID=cds.MsaT026916.1;Parent=MsaT026916.1 |
| Chr5 | exon | 31302810 | 31302878 | 31302810 | ID=MsaT026916.1.exon1;Parent=MsaT026916.1 |
| Chr5 | CDS | 31302810 | 31302878 | 31302810 | ID=cds.MsaT026916.1;Parent=MsaT026916.1 |
| Gene Sequence |
| Protein sequence |
