AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026939


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026939 MtrunA17_Chr6g0460951 86.792 212 18 2 1 202 1 212 6.38e-119 336
MsaG026939 MtrunA17_Chr7g0260971 67.961 103 33 0 38 140 13 115 8.60e-47 153
MsaG026939 MtrunA17_Chr1g0184271 60.169 118 45 1 39 156 12 127 4.13e-46 152
MsaG026939 MtrunA17_Chr8g0351701 67.647 102 33 0 39 140 23 124 6.36e-46 151
MsaG026939 MtrunA17_Chr7g0261031 67.647 102 33 0 38 139 7 108 8.35e-46 150
MsaG026939 MtrunA17_Chr1g0184281 65.347 101 35 0 39 139 15 115 2.97e-45 149
MsaG026939 MtrunA17_Chr8g0351721 59.664 119 47 1 39 156 18 136 7.46e-44 145
MsaG026939 MtrunA17_Chr5g0435641 53.782 119 55 0 38 156 6 124 1.51e-43 144
MsaG026939 MtrunA17_Chr7g0245871 61.765 102 39 0 39 140 27 128 4.97e-43 144
MsaG026939 MtrunA17_Chr6g0460941 68.182 88 28 0 39 126 7 94 7.50e-41 134
MsaG026939 MtrunA17_Chr4g0058651 55.046 109 48 1 38 146 10 117 5.31e-38 129
MsaG026939 MtrunA17_Chr6g0449641 51.724 116 53 2 38 153 8 120 1.18e-36 125
MsaG026939 MtrunA17_Chr3g0117121 54.545 110 47 2 38 147 9 115 1.76e-36 126
MsaG026939 MtrunA17_Chr7g0227511 54.808 104 46 1 38 141 10 112 1.20e-35 123
MsaG026939 MtrunA17_Chr4g0043421 41.447 152 79 3 38 183 14 161 1.21e-35 123
MsaG026939 MtrunA17_Chr2g0311601 45.378 119 64 1 27 145 1 118 1.10e-34 120
MsaG026939 MtrunA17_Chr3g0114061 43.262 141 71 2 38 170 7 146 1.11e-34 120
MsaG026939 MtrunA17_Chr3g0129071 51.786 112 53 1 37 148 45 155 1.16e-34 122
MsaG026939 MtrunA17_Chr3g0106101 50.962 104 50 1 38 141 6 108 4.41e-34 119
MsaG026939 MtrunA17_Chr7g0245861 64.444 90 30 2 39 126 12 101 7.08e-34 117
MsaG026939 MtrunA17_Chr5g0438091 41.611 149 86 1 1 149 1 148 7.23e-34 119
MsaG026939 MtrunA17_Chr6g0449651 49.138 116 56 2 38 153 66 178 7.92e-34 119
MsaG026939 MtrunA17_Chr5g0402611 54.369 103 46 1 38 140 7 108 4.28e-33 120
MsaG026939 MtrunA17_Chr5g0437401 51.456 103 49 1 38 140 10 111 4.93e-33 116
MsaG026939 MtrunA17_Chr4g0030001 38.650 163 91 4 36 191 21 181 1.16e-32 116
MsaG026939 MtrunA17_Chr5g0437571 46.218 119 61 2 38 156 5 120 2.44e-32 114
MsaG026939 MtrunA17_Chr3g0115431 52.427 103 48 1 38 140 8 109 6.50e-32 115
MsaG026939 MtrunA17_Chr3g0097221 47.009 117 58 2 38 154 6 118 1.43e-30 113
MsaG026939 MtrunA17_Chr3g0113891 48.980 98 49 1 38 135 13 109 4.10e-30 109
MsaG026939 MtrunA17_Chr5g0432451 52.778 108 50 1 37 144 41 147 8.79e-29 107
MsaG026939 MtrunA17_Chr8g0353981 58.537 82 33 1 38 119 6 86 4.19e-28 105
MsaG026939 MtrunA17_Chr1g0200201 44.348 115 61 2 40 153 73 185 1.08e-27 104
MsaG026939 MtrunA17_Chr5g0444351 44.545 110 60 1 36 145 2 110 3.29e-27 100
MsaG026939 MtrunA17_Chr7g0225111 42.727 110 62 1 36 145 2 110 3.50e-27 100
MsaG026939 MtrunA17_Chr8g0374601 45.714 105 56 1 36 140 23 126 4.24e-26 101
MsaG026939 MtrunA17_Chr8g0374591 39.815 108 64 1 33 140 2 108 1.94e-23 90.9
MsaG026939 MtrunA17_Chr8g0374581 36.641 131 77 3 34 163 3 128 7.36e-21 86.7
MsaG026939 MtrunA17_Chr4g0059901 41.584 101 58 1 40 140 11 110 3.04e-20 85.5
MsaG026939 MtrunA17_Chr8g0342351 38.614 101 61 1 40 140 13 112 4.58e-19 82.4
MsaG026939 MtrunA17_Chr2g0330201 37.624 101 62 1 40 140 20 119 1.28e-18 81.6
MsaG026939 MtrunA17_Chr6g0452691 45.679 81 42 2 40 119 10 89 4.04e-18 78.2
MsaG026939 MtrunA17_Chr6g0452671 36.634 101 63 1 40 140 12 111 9.08e-18 77.8
MsaG026939 MtrunA17_Chr4g0040831 36.190 105 63 2 40 141 16 119 1.88e-17 77.0
MsaG026939 MtrunA17_Chr6g0452661 33.945 109 67 3 36 140 3 110 4.31e-16 74.3
MsaG026939 MtrunA17_Chr4g0055441 41.975 81 45 2 40 119 3 82 5.22e-15 69.3
MsaG026939 MtrunA17_Chr2g0326041 51.852 54 25 1 36 89 30 82 5.65e-15 67.4
MsaG026939 MtrunA17_Chr5g0439111 30.247 162 92 7 36 180 3 160 8.25e-14 67.8
MsaG026939 MtrunA17_Chr2g0326051 35.714 98 52 3 85 171 5 102 8.28e-11 57.4
MsaG026939 MtrunA17_Chr4g0046201 30.682 88 60 1 40 127 7 93 9.18e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026939 AT3G03760.1 70.139 144 39 1 38 177 50 193 2.79e-64 200
MsaG026939 AT2G42430.1 54.605 152 50 2 38 170 14 165 1.37e-48 159
MsaG026939 AT3G58190.1 61.017 118 45 1 39 156 11 127 9.98e-47 153
MsaG026939 AT5G06080.1 48.611 144 65 1 38 181 6 140 7.01e-44 145
MsaG026939 AT2G42440.1 52.672 131 59 1 38 168 6 133 7.32e-43 144
MsaG026939 AT4G00220.1 63.462 104 38 0 42 145 20 123 8.66e-43 143
MsaG026939 AT2G45420.1 64.423 104 37 0 42 145 40 143 9.60e-43 144
MsaG026939 AT2G31310.1 58.824 102 42 0 38 139 6 107 4.22e-42 140
MsaG026939 AT4G00210.1 61.765 102 39 0 39 140 11 112 4.50e-41 139
MsaG026939 AT2G45410.1 49.286 140 62 3 42 179 19 151 5.12e-38 130
MsaG026939 AT3G27650.1 55.769 104 45 1 38 141 38 140 1.83e-36 125
MsaG026939 AT5G63090.4 53.211 109 50 1 38 146 10 117 1.49e-35 124
MsaG026939 AT5G63090.3 53.211 109 50 1 38 146 10 117 1.49e-35 124
MsaG026939 AT5G63090.2 53.211 109 50 1 38 146 10 117 1.49e-35 124
MsaG026939 AT5G63090.1 53.211 109 50 1 38 146 10 117 1.49e-35 124
MsaG026939 AT1G16530.1 49.541 109 53 2 37 144 12 119 1.64e-33 118
MsaG026939 AT2G30130.1 47.934 121 60 2 38 158 7 124 2.62e-33 118
MsaG026939 AT2G30340.1 47.863 117 58 2 37 153 50 163 3.46e-33 120
MsaG026939 AT2G30340.2 47.863 117 58 2 37 153 51 164 3.90e-33 120
MsaG026939 AT5G66870.1 45.070 142 73 2 38 179 6 142 4.02e-33 120
MsaG026939 AT1G07900.1 44.538 119 63 2 36 153 30 146 3.37e-32 115
MsaG026939 AT3G11090.1 48.077 104 53 1 38 141 10 112 3.57e-31 112
MsaG026939 AT2G28500.1 46.847 111 56 2 36 145 52 160 5.29e-31 113
MsaG026939 AT1G31320.1 45.378 119 64 1 27 145 1 118 1.54e-30 110
MsaG026939 AT1G65620.1 54.369 103 46 1 38 140 8 109 1.97e-29 108
MsaG026939 AT1G65620.4 54.369 103 46 1 38 140 8 109 1.97e-29 108
MsaG026939 AT1G65620.3 54.369 103 46 1 38 140 8 109 1.97e-29 108
MsaG026939 AT1G65620.5 54.369 103 46 1 38 140 8 109 1.97e-29 108
MsaG026939 AT1G65620.2 54.369 103 46 1 38 140 8 109 1.97e-29 108
MsaG026939 AT2G23660.2 45.192 104 56 1 38 141 4 106 3.51e-28 108
MsaG026939 AT2G23660.3 45.192 104 56 1 38 141 4 106 3.51e-28 108
MsaG026939 AT2G23660.1 45.192 104 56 1 38 141 4 106 3.51e-28 108
MsaG026939 AT3G26660.1 44.444 108 52 2 40 140 6 112 8.66e-28 102
MsaG026939 AT3G26620.1 44.444 108 52 2 40 140 6 112 1.41e-27 101
MsaG026939 AT2G40470.2 51.667 120 56 2 37 156 18 135 1.65e-27 103
MsaG026939 AT2G40470.1 51.667 120 56 2 37 156 43 160 1.99e-27 103
MsaG026939 AT3G50510.1 39.450 109 65 1 32 140 5 112 1.09e-23 93.6
MsaG026939 AT3G50510.2 39.450 109 65 1 32 140 5 112 1.09e-23 93.6
MsaG026939 AT3G13850.1 32.298 161 103 2 40 200 37 191 3.31e-20 85.9
MsaG026939 AT1G72980.1 38.835 103 62 1 38 140 12 113 2.75e-19 82.4
MsaG026939 AT5G35900.1 33.663 101 66 1 40 140 6 105 6.83e-16 73.2
MsaG026939 AT3G47870.1 32.673 101 67 1 40 140 37 136 1.01e-14 71.6
MsaG026939 AT1G06280.1 42.667 75 42 1 40 114 25 98 1.62e-14 69.7
MsaG026939 AT4G22700.1 32.292 96 62 2 40 134 6 99 8.87e-13 64.7

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGAAACGGCGGCTGATA+TGG 0.240934 5:+31658646 None:intergenic
AGGCTAGCTGATCCGGTTTA+TGG 0.264632 5:-31658906 MsaT026939.1:CDS
AACAACTTCAACCCTGGTTT+TGG 0.284974 5:-31658565 MsaT026939.1:CDS
CATTCTTGCTTTGCAGCAAC+AGG 0.339538 5:-31658871 MsaT026939.1:intron
GGTGGTGATGGTAGTAGTTC+GGG 0.392136 5:-31659209 MsaT026939.1:CDS
TGCATCTTTGCACCCTACTT+TGG 0.399115 5:-31659065 MsaT026939.1:CDS
TAGCCTAGCTTGAGCCTCAT+AGG 0.409914 5:+31658923 None:intergenic
ATGGTAGTAGTTCGGGAAAC+AGG 0.414773 5:-31659202 MsaT026939.1:CDS
ATTGAAACGGCGGCTGATAT+GGG 0.422968 5:+31658647 None:intergenic
GCGGCTGTGCACAAGGTGTT+TGG 0.424834 5:-31659017 MsaT026939.1:CDS
AAATAGTCGCCGCAGCTTCA+TGG 0.427210 5:+31658946 None:intergenic
TGAAGTTGTTCATATTCATA+AGG 0.429426 5:+31658578 None:intergenic
AAACTCAATTGATGAATAAG+AGG 0.436075 5:-31658702 MsaT026939.1:CDS
AGGTGGTGATGGTAGTAGTT+CGG 0.439949 5:-31659210 MsaT026939.1:CDS
TCAAGCTAGGCTAGCTGATC+CGG 0.442761 5:-31658913 MsaT026939.1:CDS
GTTGTTCATATTCATAAGGT+TGG 0.472998 5:+31658582 None:intergenic
TTGAGGAGGAAGTGTAGTAG+TGG 0.488789 5:-31659089 MsaT026939.1:CDS
GGCTACAAGCTCTGTGATGA+AGG 0.492816 5:+31658523 None:intergenic
GCACCACACGGAGATATAAT+CGG 0.498285 5:+31659122 None:intergenic
GTGGACACACAACCATAAAC+CGG 0.504022 5:+31658894 None:intergenic
TCCATTGTGAGGCCAAAACC+AGG 0.513069 5:+31658553 None:intergenic
GGTGGAAGCAGATGAGTTGT+TGG 0.513070 5:+31658606 None:intergenic
GTTCATATTCATAAGGTTGG+TGG 0.513150 5:+31658585 None:intergenic
CCAAGGTGCTGCTAAGTTTG+CGG 0.527615 5:-31659036 MsaT026939.1:CDS
CATATTCATAAGGTTGGTGG+TGG 0.528819 5:+31658588 None:intergenic
CTGTTGCTGCAAAGCAAGAA+TGG 0.532688 5:+31658872 None:intergenic
AAATGCTGCAAAGCGTGCAA+TGG 0.533586 5:-31659165 MsaT026939.1:CDS
AATATGAACAACTTCAACCC+TGG 0.543651 5:-31658571 MsaT026939.1:CDS
ACCTTGGTCAGTACCAAAGT+AGG 0.553693 5:+31659052 None:intergenic
TCGCCGCAGCTTCATGGCGA+TGG 0.568449 5:+31658952 None:intergenic
GCTGGCTGCACAAATTGAAA+CGG 0.572987 5:+31658634 None:intergenic
GCTGAGTCACAAGGTGGTGA+TGG 0.582631 5:-31659221 MsaT026939.1:CDS
TTTCCTATGAGGCTCAAGCT+AGG 0.592261 5:-31658926 MsaT026939.1:CDS
TAAGTTTGCGGCTGTGCACA+AGG 0.594059 5:-31659024 MsaT026939.1:CDS
TGCGGCGACTATTTCCTATG+AGG 0.598387 5:-31658937 MsaT026939.1:CDS
CCTTGGTCAGTACCAAAGTA+GGG 0.599955 5:+31659053 None:intergenic
CCGCAAACTTAGCAGCACCT+TGG 0.604003 5:+31659036 None:intergenic
TTGAAACGGCGGCTGATATG+GGG 0.604833 5:+31658648 None:intergenic
GATGAGTTGTTGGAATATGC+TGG 0.605347 5:+31658616 None:intergenic
CCCTACTTTGGTACTGACCA+AGG 0.613176 5:-31659053 MsaT026939.1:CDS
TAACCATCGCCATGAAGCTG+CGG 0.616919 5:-31658955 MsaT026939.1:CDS
GTGGTGCTTGCAAGTTCTTG+AGG 0.646814 5:-31659106 MsaT026939.1:CDS
GGCTGCACAAATTGAAACGG+CGG 0.671846 5:+31658637 None:intergenic
GGTGGCAGCTATGCAAGCAG+AGG 0.672106 5:-31658737 MsaT026939.1:CDS
TCTGTGATGGCTGAGTCACA+AGG 0.673947 5:-31659230 None:intergenic
GTGCTTGCAAGTTCTTGAGG+AGG 0.680170 5:-31659103 MsaT026939.1:CDS
AAGAACTTGCAAGCACCACA+CGG 0.685626 5:+31659110 None:intergenic
GCGATGGTTAGCGAGAACGT+TGG 0.688497 5:+31658968 None:intergenic
CCATTGTGAGGCCAAAACCA+GGG 0.697828 5:+31658554 None:intergenic
AAGGTGTTGTCTCCATTGTG+AGG 0.699953 5:+31658542 None:intergenic
TTGCTGCAAAGCAAGAATGG+TGG 0.702855 5:+31658875 None:intergenic
GAACCGATTATATCTCCGTG+TGG 0.743633 5:-31659125 MsaT026939.1:CDS
GTGATGGCTGAGTCACAAGG+TGG 0.752882 5:-31659227 MsaT026939.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 31658525 31659246 31658525 ID=MsaG026939
Chr5 mRNA 31658525 31659246 31658525 ID=MsaT026939.1;Parent=MsaG026939
Chr5 exon 31658525 31658758 31658525 ID=MsaT026939.1.exon2;Parent=MsaT026939.1
Chr5 CDS 31658525 31658758 31658525 ID=cds.MsaT026939.1;Parent=MsaT026939.1
Chr5 exon 31658872 31659246 31658872 ID=MsaT026939.1.exon1;Parent=MsaT026939.1
Chr5 CDS 31658872 31659246 31658872 ID=cds.MsaT026939.1;Parent=MsaT026939.1
Gene Sequence

>MsaT026939.1

ATGGCTGAGTCACAAGGTGGTGATGGTAGTAGTTCGGGAAACAGGCGCAATAAAAGCGCAAATGCTGCAAAGCGTGCAATGGCAAGAGCAACACAAGATGAACCGATTATATCTCCGTGTGGTGCTTGCAAGTTCTTGAGGAGGAAGTGTAGTAGTGGATGCATCTTTGCACCCTACTTTGGTACTGACCAAGGTGCTGCTAAGTTTGCGGCTGTGCACAAGGTGTTTGGTGCTAGCAATGTGTCGAAGCACTTGTCCAACGTTCTCGCTAACCATCGCCATGAAGCTGCGGCGACTATTTCCTATGAGGCTCAAGCTAGGCTAGCTGATCCGGTTTATGGTTGTGTGTCCACCATTCTTGCTTTGCAGCAACAGGTGGCAGCTATGCAAGCAGAGGTCTCAATGATGCAAACTCAATTGATGAATAAGAGGCTTGCATATGCAAATGCAAGTACTCTTCAAACCCCATATCAGCCGCCGTTTCAATTTGTGCAGCCAGCATATTCCAACAACTCATCTGCTTCCACCACCAACCTTATGAATATGAACAACTTCAACCCTGGTTTTGGCCTCACAATGGAGACAACACCTTCATCACAGAGCTTGTAG

Protein sequence

>MsaT026939.1

MAESQGGDGSSSGNRRNKSANAAKRAMARATQDEPIISPCGACKFLRRKCSSGCIFAPYFGTDQGAAKFAAVHKVFGASNVSKHLSNVLANHRHEAAATISYEAQARLADPVYGCVSTILALQQQVAAMQAEVSMMQTQLMNKRLAYANASTLQTPYQPPFQFVQPAYSNNSSASTTNLMNMNNFNPGFGLTMETTPSSQSL*