AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG026965

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026965 MtrunA17_Chr5g0416431 98.925 93 1 0 1 93 1 93 7.68e-61 180
MsaG026965 MtrunA17_Chr8g0362131 79.762 84 16 1 10 93 10 92 1.71e-42 134
MsaG026965 MtrunA17_Chr7g0217141 72.826 92 24 1 1 92 1 91 8.00e-41 129
MsaG026965 MtrunA17_Chr6g0453041 69.737 76 23 0 17 92 15 90 3.68e-34 112
MsaG026965 MtrunA17_Chr3g0093001 74.026 77 18 1 17 93 17 91 3.81e-34 112
MsaG026965 MtrunA17_Chr7g0237771 45.946 74 40 0 8 81 3 76 9.86e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG026965 AT1G26945.1 83.784 74 12 0 19 92 19 92 3.26e-39 125
MsaG026965 AT3G28857.1 64.894 94 30 2 1 93 1 92 3.87e-36 118
MsaG026965 AT3G47710.1 69.149 94 25 3 1 93 1 91 3.62e-35 115
MsaG026965 AT5G15160.1 73.333 75 19 1 19 92 18 92 3.39e-32 108
MsaG026965 AT1G74500.1 72.727 77 18 2 17 92 17 91 3.08e-31 105
MsaG026965 AT5G39860.1 65.385 78 26 1 17 93 15 92 1.80e-30 103

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCGATTGTCACAACTTT+TGG 0.275530 5:+32397735 MsaT026965.1:CDS
GAGCGGCTAGCGCGAAGTTC+GGG 0.276939 5:-32397218 None:intergenic
GTGCTCAAGCAGCTATTATT+AGG 0.330670 5:+32397776 MsaT026965.1:CDS
GGAGCGGCTAGCGCGAAGTT+CGG 0.362273 5:-32397219 None:intergenic
CTTATAGGTTTCAGCTACTA+AGG 0.382941 5:+32397654 MsaT026965.1:intron
AGAGGAACCACCGGATTGTC+TGG 0.389390 5:-32397141 None:intergenic
GGGGATAAGTTGTTGTAACT+TGG 0.418381 5:-32397198 None:intergenic
AATAGCTGCTTGAGCACTGT+TGG 0.496664 5:-32397770 None:intergenic
AAGCCAAAAGTTGTGACAAT+CGG 0.524888 5:-32397738 None:intergenic
AGATCTCGTTCCAGACAATC+CGG 0.532260 5:+32397131 MsaT026965.1:CDS
AAATAAATACCTTGTTGGAG+CGG 0.605762 5:-32397235 None:intergenic
TCGCGCTAGCCGCTCCAACA+AGG 0.613710 5:+32397226 MsaT026965.1:CDS
AGTGATCTCAGAGGAACCAC+CGG 0.628272 5:-32397150 None:intergenic
AGCGGCTAGCGCGAAGTTCG+GGG 0.650397 5:-32397217 None:intergenic
TCTCGTTCCAGACAATCCGG+TGG 0.728257 5:+32397134 MsaT026965.1:CDS
TTGAGCATCAGTGATCTCAG+AGG 0.728981 5:-32397159 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 32397119 32397813 32397119 ID=MsaG026965
Chr5 mRNA 32397119 32397813 32397119 ID=MsaT026965.1;Parent=MsaG026965
Chr5 exon 32397119 32397247 32397119 ID=MsaT026965.1.exon1;Parent=MsaT026965.1
Chr5 CDS 32397119 32397247 32397119 ID=cds.MsaT026965.1;Parent=MsaT026965.1
Chr5 exon 32397661 32397813 32397661 ID=MsaT026965.1.exon2;Parent=MsaT026965.1
Chr5 CDS 32397661 32397813 32397661 ID=cds.MsaT026965.1;Parent=MsaT026965.1
Gene Sequence

>MsaT026965.1

ATGTCTAGCAGAAGATCTCGTTCCAGACAATCCGGTGGTTCCTCTGAGATCACTGATGCTCAAATCACTGATCTCATTTCCAAGTTACAACAACTTATCCCCGAACTTCGCGCTAGCCGCTCCAACAAGGTTTCAGCTACTAAGGTATTGCAAGAGACTTGCAACTACATAAAAAACTTGCATAGAGAGGTTGATGATTTAAGTGACCGATTGTCACAACTTTTGGCTTCTACAGATTCCAACAGTGCTCAAGCAGCTATTATTAGGAGCTTACTTATGTAA
Protein sequence

>MsaT026965.1

MSSRRSRSRQSGGSSEITDAQITDLISKLQQLIPELRASRSNKVSATKVLQETCNYIKNLHREVDDLSDRLSQLLASTDSNSAQAAIIRSLLM*