AlfalfaGEDB | MsaG026969

M. sativa ssp. caerulea / MsaG026969

KEGG: K09338
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009965 GO:0010016 GO:0010033 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034285 GO:0042221 GO:0043565 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080022 GO:0080090 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:1905392 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026969	XP_003613642.1	98.305	236	4	0	1	236	48	283	3.87e-169	477

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026969	sp\|Q8LC03\|ATB13_ARATH	65.447	246	67	6	1	236	57	294	1.74e-100	296

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026969	tr\|G7KB59\|G7KB59_MEDTR	98.305	236	4	0	1	236	48	283	1.85e-169	477

TFs/TRs:

Gene ID	Type	Classification
MsaG026969	TF	HB-HD-ZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000465	MsaG026969	0.821234	7.904609e-53	1.796224e-50
MsaG003729	MsaG026969	0.833007	1.237021e-55	3.905614e-53
MsaG007858	MsaG026969	0.802307	1.002421e-48	1.428087e-46
MsaG017972	MsaG026969	0.800966	1.883063e-48	2.602062e-46
MsaG018105	MsaG026969	0.814444	2.652905e-51	5.057075e-49
MsaG018554	MsaG026969	0.821774	5.938407e-53	1.368860e-50
MsaG023678	MsaG026969	0.836616	1.542620e-56	5.423880e-54
MsaG023816	MsaG026969	0.808873	4.262113e-50	7.083504e-48
MsaG026969	MsaG035287	0.800713	2.119649e-48	2.912463e-46
MsaG026969	MsaG039795	0.809582	3.008628e-50	5.086452e-48
MsaG026969	MsaG039898	0.814163	3.058620e-51	5.789341e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026969	MtrunA17_Chr5g0416391	98.305	236	4	0	1	236	48	283	3.56e-173	477
MsaG026969	MtrunA17_Chr8g0362151	86.498	237	27	4	1	236	50	282	5.19e-134	378
MsaG026969	MtrunA17_Chr6g0453021	53.469	245	72	6	1	233	58	272	2.00e-73	224
MsaG026969	MtrunA17_Chr7g0217111	52.766	235	94	7	1	229	48	271	1.49e-68	212
MsaG026969	MtrunA17_Chr8g0381741	44.886	176	88	3	18	191	40	208	9.57e-39	136
MsaG026969	MtrunA17_Chr6g0450991	64.000	100	36	0	26	125	63	162	3.51e-38	134
MsaG026969	MtrunA17_Chr3g0123691	57.500	120	48	1	17	133	47	166	7.12e-38	134
MsaG026969	MtrunA17_Chr3g0119291	54.225	142	54	2	15	145	44	185	1.53e-37	132
MsaG026969	MtrunA17_Chr5g0415941	60.169	118	46	1	8	125	66	182	2.27e-37	133
MsaG026969	MtrunA17_Chr8g0362751	66.667	96	32	0	30	125	89	184	8.44e-37	130
MsaG026969	MtrunA17_Chr4g0060031	57.025	121	48	1	2	122	71	187	1.65e-36	131
MsaG026969	MtrunA17_Chr7g0265521	50.926	108	46	1	18	125	51	151	8.45e-31	113
MsaG026969	MtrunA17_Chr1g0179581	54.167	96	41	2	15	109	14	107	1.45e-26	99.4
MsaG026969	MtrunA17_Chr3g0127511	53.846	91	42	0	32	122	40	130	8.25e-26	100
MsaG026969	MtrunA17_Chr8g0346251	41.322	121	71	0	4	124	15	135	1.11e-23	95.5
MsaG026969	MtrunA17_Chr4g0054421	47.573	103	52	1	15	117	114	214	7.84e-21	88.2
MsaG026969	MtrunA17_Chr4g0055851	45.545	101	54	1	18	117	107	207	7.86e-20	85.9
MsaG026969	MtrunA17_Chr5g0403691	47.872	94	49	0	30	123	53	146	1.34e-19	84.0
MsaG026969	MtrunA17_Chr5g0403701	48.889	90	46	0	30	119	53	142	1.74e-19	83.2
MsaG026969	MtrunA17_Chr7g0258831	45.283	106	54	1	22	127	152	253	8.47e-19	83.2
MsaG026969	MtrunA17_Chr1g0175651	46.875	96	51	0	22	117	146	241	1.24e-18	82.8
MsaG026969	MtrunA17_Chr5g0400781	45.000	100	55	0	22	121	115	214	1.28e-18	82.4
MsaG026969	MtrunA17_Chr8g0335131	48.913	92	47	0	30	121	146	237	1.95e-18	82.4
MsaG026969	MtrunA17_Chr2g0308611	41.071	112	65	1	6	117	177	287	1.05e-17	80.9
MsaG026969	MtrunA17_Chr1g0151971	47.727	88	46	0	30	117	130	217	1.23e-17	79.7
MsaG026969	MtrunA17_Chr5g0399491	43.750	96	54	0	22	117	128	223	7.54e-17	77.8
MsaG026969	MtrunA17_Chr4g0071411	44.706	85	47	0	33	117	91	175	1.79e-16	75.5
MsaG026969	MtrunA17_Chr4g0044091	46.591	88	47	0	30	117	65	152	6.58e-16	73.6
MsaG026969	MtrunA17_Chr3g0135331	43.617	94	53	0	8	101	123	216	8.36e-16	75.1
MsaG026969	MtrunA17_Chr2g0299031	38.182	110	65	1	11	117	60	169	7.99e-14	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG026969	AT1G69780.1	65.447	246	67	6	1	236	57	294	1.77e-101	296
MsaG026969	AT1G26960.1	61.712	222	59	6	17	236	58	255	2.63e-85	254
MsaG026969	AT3G01220.1	52.941	221	81	6	15	229	70	273	1.35e-67	210
MsaG026969	AT5G15150.1	47.137	227	80	7	17	229	100	300	1.72e-56	182
MsaG026969	AT5G65310.2	50.602	166	75	3	18	176	39	204	1.65e-41	143
MsaG026969	AT5G65310.1	50.602	166	75	3	18	176	57	222	2.70e-41	143
MsaG026969	AT4G40060.1	57.143	133	52	3	11	139	39	170	8.10e-38	134
MsaG026969	AT2G22430.1	67.010	97	32	0	29	125	61	157	1.55e-37	133
MsaG026969	AT3G01470.1	63.000	100	37	0	26	125	64	163	9.73e-37	130
MsaG026969	AT1G27045.1	44.737	152	77	2	4	148	41	192	4.56e-28	107
MsaG026969	AT1G27045.5	45.517	145	72	2	11	148	5	149	7.36e-28	105
MsaG026969	AT1G27045.3	45.517	145	72	2	11	148	5	149	7.36e-28	105
MsaG026969	AT1G27045.2	48.800	125	58	1	30	148	17	141	2.87e-27	103
MsaG026969	AT2G46680.1	52.688	93	44	0	31	123	33	125	1.58e-26	103
MsaG026969	AT1G27045.4	49.167	120	60	1	4	122	41	160	1.89e-26	103
MsaG026969	AT2G46680.2	53.333	90	42	0	31	120	33	122	9.28e-26	101
MsaG026969	AT3G61890.1	51.111	90	44	0	33	122	33	122	2.85e-24	97.1
MsaG026969	AT5G03790.1	51.136	88	43	0	30	117	77	164	5.63e-24	96.3
MsaG026969	AT2G18550.1	39.157	166	68	5	20	177	48	188	2.47e-23	94.4
MsaG026969	AT5G03790.2	52.500	80	38	0	30	109	77	156	2.70e-22	90.9
MsaG026969	AT4G36740.2	48.544	103	46	1	21	123	47	142	7.63e-22	90.1
MsaG026969	AT4G36740.1	48.544	103	46	1	21	123	46	141	8.56e-22	90.1
MsaG026969	AT2G36610.1	41.379	116	58	2	12	119	55	168	2.20e-21	88.2
MsaG026969	AT5G66700.1	46.552	116	53	2	17	125	60	173	4.50e-21	88.6
MsaG026969	AT5G53980.1	45.545	101	55	0	25	125	6	106	1.06e-20	85.5
MsaG026969	AT3G60390.1	45.545	101	55	0	17	117	149	249	5.75e-19	84.3
MsaG026969	AT4G37790.1	45.000	100	55	0	18	117	114	213	1.95e-18	82.4
MsaG026969	AT2G01430.1	44.554	101	56	0	17	117	126	226	3.77e-18	81.6
MsaG026969	AT5G47370.1	43.434	99	56	0	19	117	119	217	4.11e-18	81.6
MsaG026969	AT5G06710.1	44.792	96	53	0	22	117	182	277	1.37e-17	80.9
MsaG026969	AT2G22800.1	44.792	96	53	0	22	117	105	200	4.90e-17	78.6
MsaG026969	AT4G17460.1	42.708	96	55	0	22	117	127	222	1.30e-16	77.4
MsaG026969	AT2G44910.1	46.591	88	47	0	30	117	163	250	3.16e-16	76.6
MsaG026969	AT1G70920.1	37.600	125	69	2	9	133	50	165	3.13e-15	72.4
MsaG026969	AT1G70920.2	37.600	125	69	2	9	133	20	135	4.54e-15	71.2
MsaG026969	AT4G16780.1	40.625	96	57	0	22	117	121	216	5.06e-14	70.1
MsaG026969	AT2G01430.2	51.515	66	32	0	17	82	126	191	3.84e-13	66.6
MsaG026969	AT5G06710.4	51.667	60	29	0	22	81	182	241	1.30e-11	63.2

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTCTTTCCACCATCTTCT+AGG	0.193298	5:+32440839	MsaT026969.1:CDS
ATCCTTCTGTTTCTTTGTTC+AGG	0.195256	5:-32440711	None:intergenic
TGGATGATCAAGCTGGATTT+TGG	0.253214	5:+32440974	MsaT026969.1:CDS
TGACCATTTGATCAATCTTT+TGG	0.263126	5:-32440918	None:intergenic
CCACCATCTTCTAGGCCTTC+AGG	0.272484	5:+32440847	MsaT026969.1:CDS
TTTCTGGCCTTGAAGAAGTT+TGG	0.289017	5:-32440888	None:intergenic
GAAGAAGAGGAGGCTTAATA+TGG	0.330137	5:+32439548	MsaT026969.1:CDS
AACCTAGACAAGTTGCTATA+TGG	0.331625	5:+32439661	MsaT026969.1:CDS
CTATATGGTTTCAAAATAGA+AGG	0.336540	5:+32439676	MsaT026969.1:CDS
ATGCCAAAAGATTGATCAAA+TGG	0.403921	5:+32440915	MsaT026969.1:CDS
TCATCTGATAAATCTTCTTC+AGG	0.406192	5:-32439507	None:intergenic
GAAAGAGGACTATCACTTGC+TGG	0.446270	5:-32440790	None:intergenic
TTCAAAATAGAAGGGCTAGA+TGG	0.455440	5:+32439685	MsaT026969.1:CDS
TTCAGGTTGATTGATTCTGT+TGG	0.470903	5:-32440694	None:intergenic
CCTAGAAGATGGTGGAAAGA+AGG	0.480852	5:-32440839	None:intergenic
GAAGAAGATTTATCAGATGA+TGG	0.491350	5:+32439510	MsaT026969.1:CDS
AGGGTCTGCTTGTTGTATGT+TGG	0.541270	5:-32440819	None:intergenic
CCACCTGAAGGCCTAGAAGA+TGG	0.542223	5:-32440850	None:intergenic
CTAGAAGATGGTGGAAAGAA+GGG	0.543462	5:-32440838	None:intergenic
TCAGATGATGGATCACAAGC+TGG	0.546396	5:+32439522	MsaT026969.1:CDS
CACAAGCTGGAGAGAAGAAG+AGG	0.557400	5:+32439535	MsaT026969.1:CDS
AGCTGAGCAATTCCACCTGA+AGG	0.558857	5:-32440862	None:intergenic
AACCATATAGCAACTTGTCT+AGG	0.569003	5:-32439663	None:intergenic
TGTGGCATGGATGATCAAGC+TGG	0.572558	5:+32440967	MsaT026969.1:CDS
TATATGGTTTCAAAATAGAA+GGG	0.590755	5:+32439677	MsaT026969.1:CDS
AAATGTTGTTGCTCCAACCA+TGG	0.592621	5:-32440997	None:intergenic
GTAATAAGCTTGAGCCTGAG+AGG	0.611232	5:+32439607	MsaT026969.1:CDS
AAGCTGGAGAGAAGAAGAGG+AGG	0.613532	5:+32439538	MsaT026969.1:CDS
GAACAGTTCAGATATCAAGT+TGG	0.615882	5:+32440753	MsaT026969.1:CDS
TAGATGGAAGACAAAGCAGT+TGG	0.627956	5:+32439701	MsaT026969.1:CDS
CCTGAAGGCCTAGAAGATGG+TGG	0.646677	5:-32440847	None:intergenic
GTATGTTGGTGTGTAGAAAG+AGG	0.664598	5:-32440805	None:intergenic
CCATCTTCTAGGCCTTCAGG+TGG	0.673245	5:+32440850	MsaT026969.1:CDS
AACCTGAACAAAGAAACAGA+AGG	0.692102	5:+32440709	MsaT026969.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	32439462	32441026	32439462	ID=MsaG026969
Chr5	mRNA	32439462	32441026	32439462	ID=MsaT026969.1;Parent=MsaG026969
Chr5	exon	32439462	32439815	32439462	ID=MsaT026969.1.exon1;Parent=MsaT026969.1
Chr5	CDS	32439462	32439815	32439462	ID=cds.MsaT026969.1;Parent=MsaT026969.1
Chr5	exon	32440670	32441026	32440670	ID=MsaT026969.1.exon2;Parent=MsaT026969.1
Chr5	CDS	32440670	32441026	32440670	ID=cds.MsaT026969.1;Parent=MsaT026969.1

Gene Sequence

>MsaT026969.1

ATGTCATTTTCATCAGGGATTGAACTTGGAGAAGAAGCAAATATACCTGAAGAAGATTTATCAGATGATGGATCACAAGCTGGAGAGAAGAAGAGGAGGCTTAATATGGAACAAGTGAAGACACTTGAGAAGAGTTTTGAACTTGGTAATAAGCTTGAGCCTGAGAGGAAAATGCAGCTTGCAAGAGCTCTTAACTTGCAACCTAGACAAGTTGCTATATGGTTTCAAAATAGAAGGGCTAGATGGAAGACAAAGCAGTTGGAAAAAGACTATGATGTTCTTAAAAGACAATATGATGCTATTAAGTTAGATAATGATGCACTTCAAGCTCAGAACCAAAAACTTCAAGCTGAGATATTGGCATTGAAAAATAGAGAACCAACAGAATCAATCAACCTGAACAAAGAAACAGAAGGATCAAGCAGCAATAGAAGTGAGAACAGTTCAGATATCAAGTTGGATATGTCAAGAACACCAGCAAGTGATAGTCCTCTTTCTACACACCAACATACAACAAGCAGACCCTTCTTTCCACCATCTTCTAGGCCTTCAGGTGGAATTGCTCAGCTTTTCCAAACTTCTTCAAGGCCAGAAATTCAATGCCAAAAGATTGATCAAATGGTCAAAGAAGAAAGTTTAAGCAATATGTTTTGTGGCATGGATGATCAAGCTGGATTTTGGCCATGGTTGGAGCAACAACATTTCAATTGA

Protein sequence

>MsaT026969.1

MSFSSGIELGEEANIPEEDLSDDGSQAGEKKRRLNMEQVKTLEKSFELGNKLEPERKMQLARALNLQPRQVAIWFQNRRARWKTKQLEKDYDVLKRQYDAIKLDNDALQAQNQKLQAEILALKNREPTESINLNKETEGSSSNRSENSSDIKLDMSRTPASDSPLSTHQHTTSRPFFPPSSRPSGGIAQLFQTSSRPEIQCQKIDQMVKEESLSNMFCGMDDQAGFWPWLEQQHFN*