AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027011


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027011 MtrunA17_Chr5g0415941 94.720 322 8 1 1 322 12 324 0.0 617
MsaG027011 MtrunA17_Chr8g0362751 52.134 328 96 8 1 322 14 286 2.22e-95 284
MsaG027011 MtrunA17_Chr4g0060031 48.311 296 130 8 34 322 56 335 1.45e-75 235
MsaG027011 MtrunA17_Chr6g0450991 46.701 197 91 3 76 269 67 252 4.93e-42 147
MsaG027011 MtrunA17_Chr8g0381741 60.656 122 44 2 66 186 43 161 8.82e-40 142
MsaG027011 MtrunA17_Chr6g0453021 42.975 242 96 10 28 250 41 259 2.93e-39 139
MsaG027011 MtrunA17_Chr3g0123691 60.526 114 41 1 59 172 47 156 4.03e-38 137
MsaG027011 MtrunA17_Chr3g0119291 53.901 141 49 2 76 201 59 198 5.52e-38 135
MsaG027011 MtrunA17_Chr8g0362151 53.333 135 61 1 39 171 38 172 5.74e-37 134
MsaG027011 MtrunA17_Chr5g0416391 60.169 118 46 1 55 171 55 172 8.68e-37 133
MsaG027011 MtrunA17_Chr7g0217111 58.559 111 44 1 63 171 65 175 2.09e-32 121
MsaG027011 MtrunA17_Chr7g0265521 55.670 97 36 1 76 172 63 152 7.86e-27 105
MsaG027011 MtrunA17_Chr1g0179581 55.208 96 36 1 71 166 23 111 2.36e-26 100
MsaG027011 MtrunA17_Chr3g0127511 54.945 91 41 0 78 168 40 130 8.09e-26 103
MsaG027011 MtrunA17_Chr8g0346251 48.544 103 52 1 80 182 45 146 9.66e-26 103
MsaG027011 MtrunA17_Chr5g0403701 60.563 71 28 0 76 146 53 123 7.69e-20 86.3
MsaG027011 MtrunA17_Chr4g0055851 46.281 121 52 4 56 174 103 212 2.65e-19 86.3
MsaG027011 MtrunA17_Chr5g0399491 39.535 172 77 6 9 174 78 228 2.68e-19 86.7
MsaG027011 MtrunA17_Chr5g0403691 60.563 71 28 0 76 146 53 123 2.69e-19 85.1
MsaG027011 MtrunA17_Chr5g0400781 45.794 107 49 2 64 170 113 210 1.40e-17 81.3
MsaG027011 MtrunA17_Chr4g0071411 46.154 91 42 1 79 169 91 174 1.58e-17 80.1
MsaG027011 MtrunA17_Chr8g0335131 48.515 101 44 2 76 175 146 239 3.13e-17 80.9
MsaG027011 MtrunA17_Chr2g0308611 44.340 106 52 1 64 169 188 286 4.21e-17 80.9
MsaG027011 MtrunA17_Chr7g0258831 47.872 94 42 1 76 169 160 246 4.45e-17 80.1
MsaG027011 MtrunA17_Chr1g0175651 46.809 94 43 1 76 169 154 240 3.02e-16 77.8
MsaG027011 MtrunA17_Chr4g0054421 43.925 107 52 2 63 169 115 213 8.35e-16 76.3
MsaG027011 MtrunA17_Chr1g0151971 46.316 95 44 1 76 170 130 217 1.48e-15 75.9
MsaG027011 MtrunA17_Chr4g0044091 37.879 132 61 3 76 206 65 176 1.79e-15 73.9
MsaG027011 MtrunA17_Chr3g0135331 48.611 72 37 0 76 147 145 216 3.65e-13 68.9
MsaG027011 MtrunA17_Chr2g0299031 41.489 94 48 1 76 169 82 168 2.21e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027011 AT3G01470.1 44.444 216 85 6 40 246 30 219 2.03e-42 148
MsaG027011 AT4G40060.1 54.615 130 53 2 65 191 45 171 3.68e-41 145
MsaG027011 AT2G22430.1 68.041 97 31 0 76 172 62 158 5.04e-39 140
MsaG027011 AT1G69780.1 64.545 110 38 1 63 171 71 180 1.65e-38 138
MsaG027011 AT5G65310.2 68.041 97 31 0 76 172 54 150 1.99e-38 138
MsaG027011 AT5G65310.1 68.041 97 31 0 76 172 72 168 2.18e-38 138
MsaG027011 AT3G01220.1 49.068 161 68 3 39 187 46 204 2.39e-37 135
MsaG027011 AT5G15150.1 60.000 115 44 1 55 169 96 208 1.63e-36 134
MsaG027011 AT1G26960.1 59.091 110 44 1 63 171 57 166 7.71e-34 125
MsaG027011 AT1G27045.1 46.939 147 60 3 26 172 36 164 1.08e-32 121
MsaG027011 AT1G27045.5 60.825 97 38 0 76 172 25 121 1.07e-31 117
MsaG027011 AT1G27045.3 60.825 97 38 0 76 172 25 121 1.07e-31 117
MsaG027011 AT1G27045.2 60.825 97 38 0 76 172 17 113 1.41e-31 117
MsaG027011 AT1G27045.4 46.259 147 60 4 26 172 36 163 2.22e-30 115
MsaG027011 AT2G46680.1 49.541 109 55 0 72 180 28 136 3.30e-28 110
MsaG027011 AT3G61890.1 53.191 94 44 0 79 172 33 126 4.91e-27 106
MsaG027011 AT2G46680.2 44.800 125 68 1 72 196 28 151 8.03e-27 106
MsaG027011 AT5G03790.1 53.608 97 38 1 76 172 77 166 1.81e-25 102
MsaG027011 AT5G03790.2 54.839 93 35 1 76 168 77 162 2.38e-24 98.2
MsaG027011 AT2G36610.1 47.222 108 42 2 73 172 68 168 7.29e-24 96.7
MsaG027011 AT4G36740.1 51.429 105 42 2 76 180 55 150 3.47e-22 93.2
MsaG027011 AT4G36740.2 51.429 105 42 2 76 180 56 151 3.51e-22 93.2
MsaG027011 AT2G18550.1 45.378 119 58 1 76 194 61 172 1.80e-21 91.3
MsaG027011 AT5G53980.1 46.392 97 52 0 76 172 11 107 7.38e-21 87.8
MsaG027011 AT5G66700.1 58.333 72 30 0 76 147 71 142 3.82e-20 87.8
MsaG027011 AT5G06710.1 48.421 95 42 1 76 170 190 277 4.53e-17 81.3
MsaG027011 AT4G17460.1 45.217 115 49 4 64 175 125 228 1.16e-16 79.3
MsaG027011 AT2G01430.1 39.706 136 64 4 39 169 103 225 1.81e-16 78.6
MsaG027011 AT5G47370.1 39.695 131 64 3 46 175 107 223 2.40e-16 78.2
MsaG027011 AT2G44910.1 47.000 100 45 2 76 174 163 255 4.71e-16 77.8
MsaG027011 AT3G60390.1 47.000 100 45 2 76 174 162 254 7.19e-16 77.4
MsaG027011 AT2G22800.1 45.263 95 45 1 76 170 113 200 1.25e-15 76.3
MsaG027011 AT4G37790.1 44.681 94 45 1 76 169 126 212 2.37e-15 75.5
MsaG027011 AT4G16780.1 46.000 100 46 2 76 174 129 221 2.40e-15 75.5
MsaG027011 AT5G06710.4 58.491 53 22 0 76 128 190 242 1.56e-12 67.0
MsaG027011 AT1G70920.1 43.299 97 48 1 73 169 66 155 2.10e-12 65.9
MsaG027011 AT1G70920.2 43.299 97 48 1 73 169 36 125 2.48e-12 65.1
MsaG027011 AT2G01430.2 41.053 95 45 3 39 128 103 191 4.11e-12 65.1

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAAATTCTTGTAATTT+TGG 0.015177 5:+33273893 MsaT027011.1:CDS
TCAATTGCAAATTCAGAAAA+TGG 0.146571 5:+33273602 MsaT027011.1:CDS
CTGAAGCCCTTGAATCTCTT+TGG 0.177337 5:+33272218 MsaT027011.1:CDS
TGAACCTTGCCCTTCTGTTT+TGG 0.204900 5:-33272942 None:intergenic
GCAAGTTGAACTTTCCTATC+AGG 0.270065 5:-33272877 None:intergenic
TAGCAAACATCTTCAACTTT+TGG 0.273698 5:-33273860 None:intergenic
TTCTCTTTCTCTATATCTCT+TGG 0.295443 5:-33273509 None:intergenic
TGAAGCCCTTGAATCTCTTT+GGG 0.300479 5:+33272219 MsaT027011.1:CDS
TGAGGTGAATTGATTGCTTC+TGG 0.314138 5:-33273551 None:intergenic
CAAAGTGTCACTCCATAATA+TGG 0.334237 5:+33273628 MsaT027011.1:CDS
AACCAAGGCAAGTTGCTATA+TGG 0.341312 5:+33272917 MsaT027011.1:CDS
GTGGAAATTTAAATTCAATA+AGG 0.341810 5:-33273950 None:intergenic
CAAAAGGTTTGATCTTCAAC+AGG 0.362492 5:-33273920 None:intergenic
TTTCTCAAGTTGTTGAAAGA+AGG 0.381022 5:-33272731 None:intergenic
TCTCTTTCTCTATATCTCTT+GGG 0.387856 5:-33273508 None:intergenic
GATAGCCCACATTGCAATGA+TGG 0.423802 5:+33273707 MsaT027011.1:CDS
ATTGGATCTTTGTTGTTATG+AGG 0.430991 5:-33273569 None:intergenic
TTCTCAAGTTGTTGAAAGAA+GGG 0.453337 5:-33272730 None:intergenic
GCAATTGAGATTATATCCAT+TGG 0.455432 5:-33273587 None:intergenic
CATATTATGGAGTGACACTT+TGG 0.455719 5:-33273627 None:intergenic
GTTCAACTTGCAAAAGAGCT+TGG 0.481261 5:+33272889 MsaT027011.1:CDS
AACCATATAGCAACTTGCCT+TGG 0.481745 5:-33272919 None:intergenic
TGTGAGAAATCTGAATGATC+AGG 0.485138 5:-33273782 None:intergenic
CAATTGCAAATTCAGAAAAT+GGG 0.487982 5:+33273603 MsaT027011.1:CDS
GAATGACAAGTTAAAAGAAG+AGG 0.495747 5:+33273050 MsaT027011.1:CDS
TCAATAAGGCCAGAAACAAA+AGG 0.504416 5:-33273936 None:intergenic
AAAACAAGCTTGAACCTGAT+AGG 0.509669 5:+33272863 MsaT027011.1:CDS
AAGAGCTTGGTTTGCAACCA+AGG 0.513381 5:+33272902 MsaT027011.1:CDS
AGATCACCTTGAAAAGAAGC+AGG 0.525676 5:-33272250 None:intergenic
TGTGAACCCAAAGAGATTCA+AGG 0.525903 5:-33272225 None:intergenic
ACCATCAACAAGGAAAGAAA+AGG 0.538035 5:+33272791 MsaT027011.1:CDS
TGTTCCCATCATTGCAATGT+GGG 0.548653 5:-33273712 None:intergenic
TCATAACAACAAAGATCCAA+TGG 0.563990 5:+33273571 MsaT027011.1:CDS
GGATTCAGGTTCAAATTCAG+TGG 0.566281 5:+33272660 MsaT027011.1:intron
TCAAGGGCTTCAGAAGTACA+AGG 0.569376 5:-33272208 None:intergenic
CTATATGGTTCCAAAACAGA+AGG 0.583686 5:+33272932 MsaT027011.1:CDS
ATAGCCCACATTGCAATGAT+GGG 0.583931 5:+33273708 MsaT027011.1:CDS
GTGAACCCAAAGAGATTCAA+GGG 0.591669 5:-33272224 None:intergenic
CTTGAGAAAGAAGAGAATTG+TGG 0.599550 5:+33272745 MsaT027011.1:CDS
GATCACCTTGAAAAGAAGCA+GGG 0.602083 5:-33272249 None:intergenic
AAAGCATGTGAAGAATCTGT+AGG 0.619583 5:-33273755 None:intergenic
ATCAACAAGGAAAGAAAAGG+AGG 0.621166 5:+33272794 MsaT027011.1:CDS
TTGTTCCCATCATTGCAATG+TGG 0.645404 5:-33273713 None:intergenic
ATCACCTTGAAAAGAAGCAG+GGG 0.648333 5:-33272248 None:intergenic
GGTTCCAAAACAGAAGGGCA+AGG 0.651221 5:+33272938 MsaT027011.1:CDS
TATATGGTTCCAAAACAGAA+GGG 0.680215 5:+33272933 MsaT027011.1:CDS
AAAAGGTTTGATCTTCAACA+GGG 0.685076 5:-33273919 None:intergenic
GAAGCATGCTACCATCAACA+AGG 0.695864 5:+33272781 MsaT027011.1:CDS
CAAGGGCTTCAGAAGTACAA+GGG 0.720149 5:-33272207 None:intergenic
TGGTGGTGGAAGCAACAGAG+TGG 0.722441 5:+33272696 MsaT027011.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 33272178 33273958 33272178 ID=MsaG027011
Chr5 mRNA 33272178 33273958 33272178 ID=MsaT027011.1;Parent=MsaG027011
Chr5 exon 33272178 33272265 33272178 ID=MsaT027011.1.exon1;Parent=MsaT027011.1
Chr5 CDS 33272178 33272265 33272178 ID=cds.MsaT027011.1;Parent=MsaT027011.1
Chr5 exon 33272668 33273071 33272668 ID=MsaT027011.1.exon2;Parent=MsaT027011.1
Chr5 CDS 33272668 33273071 33272668 ID=cds.MsaT027011.1;Parent=MsaT027011.1
Chr5 exon 33273482 33273958 33273482 ID=MsaT027011.1.exon3;Parent=MsaT027011.1
Chr5 CDS 33273482 33273958 33273482 ID=cds.MsaT027011.1;Parent=MsaT027011.1
Gene Sequence

>MsaT027011.1

ATGTCACTTTTGCTTCAAAATGAAAGACTCCCTTGTACTTCTGAAGCCCTTGAATCTCTTTGGGTTCACACCCCTGCTTCTTTTCAAGGTTCAAATTCAGTGGTTAATTTTGAGAATGGTGGTGGAAGCAACAGAGTGGTAACAGATAGACCCTTCTTTCAACAACTTGAGAAAGAAGAGAATTGTGGTGATGAAGATTATGAAGCATGCTACCATCAACAAGGAAAGAAAAGGAGGCTTTCAAGTGAACAAGTTCAATTTCTTGAAAAGAGTTTTGAGGTAGAAAACAAGCTTGAACCTGATAGGAAAGTTCAACTTGCAAAAGAGCTTGGTTTGCAACCAAGGCAAGTTGCTATATGGTTCCAAAACAGAAGGGCAAGGTTCAAAACTAAACAGCTTGAAAAAGATTATGGCACATTGAAAGCTAGCTTTGATAGTCTCAAAGATGATTATGATAATCTTCTTCAAGAGAATGACAAGTTAAAAGAAGAGGTGAATTCTCTGAAGAACAAATTGATCCCAAGAGATATAGAGAAAGAGAATTCAGATGACAAATCATCACCAGAAGCAATCAATTCACCTCATAACAACAAAGATCCAATGGATATAATCTCAATTGCAAATTCAGAAAATGGGTCCAAAGTGTCACTCCATAATATGGTACTAAAATGTAAGCAAGAAGATGCCAACTCAGCTAAAAGTGATGTGCTTGATTCTGATAGCCCACATTGCAATGATGGGAACAATCTTTCTTCTTTCATAGAGCCTACAGATTCTTCACATGCTTTTGAACCTGATCATTCAGATTTCTCACAAGATGAAGAAGATAATGATAACTTGAGTCATAATCTTTTGACTCTTCCTTGCTTACCAAAAGTTGAAGATGTTTGCTATGATGACCCACATGAAAATTCTTGTAATTTTGGGTTCCCTGTTGAAGATCAAACCTTTTGTTTCTGGCCTTATTGA

Protein sequence

>MsaT027011.1

MSLLLQNERLPCTSEALESLWVHTPASFQGSNSVVNFENGGGSNRVVTDRPFFQQLEKEENCGDEDYEACYHQQGKKRRLSSEQVQFLEKSFEVENKLEPDRKVQLAKELGLQPRQVAIWFQNRRARFKTKQLEKDYGTLKASFDSLKDDYDNLLQENDKLKEEVNSLKNKLIPRDIEKENSDDKSSPEAINSPHNNKDPMDIISIANSENGSKVSLHNMVLKCKQEDANSAKSDVLDSDSPHCNDGNNLSSFIEPTDSSHAFEPDHSDFSQDEEDNDNLSHNLLTLPCLPKVEDVCYDDPHENSCNFGFPVEDQTFCFWPY*