AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027211


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027211 MtrunA17_Chr0c01g0489461 98.980 392 1 2 12 402 9 398 0.0 796
MsaG027211 MtrunA17_Chr3g0132471 46.053 228 100 5 180 396 97 312 1.68e-55 186
MsaG027211 MtrunA17_Chr1g0191571 47.087 206 104 2 192 396 129 330 7.66e-53 179
MsaG027211 MtrunA17_Chr8g0366551 41.241 274 122 7 154 402 60 319 1.41e-51 175
MsaG027211 MtrunA17_Chr8g0345871 53.555 211 79 4 188 397 170 362 1.47e-51 176
MsaG027211 MtrunA17_Chr7g0273081 45.175 228 106 6 181 402 98 312 1.49e-51 175
MsaG027211 MtrunA17_Chr2g0287761 45.408 196 97 2 199 394 1 186 6.25e-48 162
MsaG027211 MtrunA17_Chr1g0158941 38.053 226 129 1 177 391 65 290 5.35e-47 162
MsaG027211 MtrunA17_Chr5g0426851 43.981 216 112 6 180 392 79 288 6.10e-47 162
MsaG027211 MtrunA17_Chr3g0126381 35.052 194 93 4 231 391 4 197 1.99e-26 105
MsaG027211 MtrunA17_Chr8g0366751 27.273 231 133 9 174 398 439 640 2.22e-15 78.2
MsaG027211 MtrunA17_Chr5g0411341 27.700 213 125 6 174 380 470 659 3.60e-14 74.3
MsaG027211 MtrunA17_Chr4g0052821 25.128 195 129 3 201 393 291 470 1.90e-13 71.6
MsaG027211 MtrunA17_Chr4g0052841 24.500 200 134 3 201 398 293 477 8.94e-13 69.7
MsaG027211 MtrunA17_Chr3g0126361 45.977 87 35 3 156 230 46 132 1.74e-12 64.7
MsaG027211 MtrunA17_Chr1g0185621 28.846 156 94 5 201 355 476 615 6.73e-12 67.4
MsaG027211 MtrunA17_Chr5g0400621 25.150 167 102 3 201 367 158 301 5.42e-11 63.5
MsaG027211 MtrunA17_Chr3g0131691 31.034 174 93 8 201 371 299 448 5.69e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027211 AT3G24140.2 60.349 401 105 12 47 402 11 402 4.55e-140 405
MsaG027211 AT3G24140.1 60.100 401 106 12 47 402 11 402 2.93e-139 404
MsaG027211 AT3G06120.1 43.564 202 101 3 199 396 1 193 4.85e-48 162
MsaG027211 AT1G22490.1 43.662 213 106 4 189 400 105 304 1.45e-47 164
MsaG027211 AT1G72210.1 42.922 219 106 5 189 402 115 319 2.66e-47 164
MsaG027211 AT1G22490.2 45.989 187 88 3 188 374 104 277 1.75e-44 157
MsaG027211 AT1G72210.2 43.684 190 89 4 189 374 115 290 1.30e-42 152
MsaG027211 AT2G46810.2 42.157 204 96 4 192 393 114 297 9.71e-41 146
MsaG027211 AT2G46810.3 42.157 204 96 4 192 393 174 357 5.51e-40 146
MsaG027211 AT4G01460.1 40.758 211 114 4 189 398 105 305 6.95e-40 144
MsaG027211 AT4G01460.2 40.758 211 114 4 189 398 105 305 6.95e-40 144
MsaG027211 AT2G46810.1 42.157 204 96 4 192 393 186 369 7.85e-40 146
MsaG027211 AT5G46690.1 41.964 224 114 4 188 396 77 299 6.86e-39 142
MsaG027211 AT2G46810.4 45.109 184 80 3 192 374 114 277 1.45e-38 141
MsaG027211 AT3G61950.2 40.580 207 100 4 189 393 117 302 2.64e-38 140
MsaG027211 AT3G61950.6 42.487 193 89 3 189 380 117 288 4.56e-38 139
MsaG027211 AT3G61950.1 40.580 207 100 4 189 393 168 353 1.10e-37 140
MsaG027211 AT3G61950.3 42.487 193 89 3 189 380 168 339 2.45e-37 139
MsaG027211 AT5G46690.2 43.500 200 98 3 189 374 78 276 1.06e-35 133
MsaG027211 AT5G65320.1 36.321 212 122 4 189 397 92 293 1.33e-30 119
MsaG027211 AT5G65320.2 38.503 187 104 3 189 374 92 268 2.61e-27 110
MsaG027211 AT5G53210.2 65.714 70 24 0 197 266 100 169 3.31e-25 105
MsaG027211 AT5G53210.2 50.000 74 37 0 304 377 274 347 3.88e-15 76.6
MsaG027211 AT5G53210.1 65.714 70 24 0 197 266 100 169 4.65e-25 105
MsaG027211 AT5G53210.1 46.591 88 47 0 304 391 274 361 2.28e-18 86.3
MsaG027211 AT4G17880.1 27.354 223 126 7 157 376 380 569 3.18e-15 77.8
MsaG027211 AT1G32640.1 24.503 302 158 10 84 375 364 605 4.05e-15 77.8
MsaG027211 AT4G37850.2 25.573 262 161 6 141 394 90 325 7.77e-13 69.3
MsaG027211 AT4G37850.1 25.573 262 161 6 141 394 90 325 7.77e-13 69.3
MsaG027211 AT3G61950.4 66.667 42 14 0 189 230 168 209 1.44e-12 67.0
MsaG027211 AT3G61950.5 66.667 42 14 0 189 230 168 209 1.44e-12 67.0
MsaG027211 AT5G46760.1 24.898 245 150 7 136 376 358 572 3.33e-12 68.6
MsaG027211 AT2G16910.1 27.320 194 136 4 201 392 315 505 8.45e-11 64.3

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGATTCTTCGCGGTTTA+AGG 0.091676 5:+37486012 None:intergenic
GTGATGCAGTTTGCAGATTT+TGG 0.187925 5:-37486046 MsaT027211.1:CDS
GGTGATCAAGCTTCTATAAT+TGG 0.199677 5:-37485148 MsaT027211.1:CDS
TCACTTGTCTTAACAGTTCT+TGG 0.201116 5:+37485749 None:intergenic
CACCACTCAACATCAAATTT+TGG 0.276248 5:+37485933 None:intergenic
CCTCCTGAAGTTTGTGTTTG+AGG 0.289960 5:+37486112 None:intergenic
TCGCGGTTTAAGGCTAATTT+AGG 0.318430 5:+37486022 None:intergenic
ACTAGCTTCATCTGGTCATT+TGG 0.371823 5:+37484968 None:intergenic
AAGCTAGTTGAGATGGAAAC+TGG 0.382861 5:-37484953 MsaT027211.1:CDS
GTGATCAAGCTTCTATAATT+GGG 0.386419 5:-37485147 MsaT027211.1:CDS
AACCAGGCATGAGGGATCTA+AGG 0.393915 5:+37485648 None:intergenic
GATGTAGAAGTGAAACTTCT+AGG 0.406812 5:-37484890 MsaT027211.1:CDS
TGGTCATTTGGTAAAGTAGC+TGG 0.411525 5:+37484980 None:intergenic
TATAGAGTTTGTGAGGGAAT+TGG 0.411761 5:-37485119 MsaT027211.1:CDS
GCCTTAAACCGCGAAGAATC+AGG 0.418841 5:-37486013 MsaT027211.1:CDS
CCATCTCAACTAGCTTCATC+TGG 0.419409 5:+37484960 None:intergenic
AAGCACAATCAAAACAAGTT+GGG 0.436877 5:-37485045 MsaT027211.1:CDS
TGCAGAGAGTAAGTCATGTT+TGG 0.450615 5:-37484915 MsaT027211.1:CDS
TCTATCTTATTGTTTAAGAC+AGG 0.457469 5:+37485959 None:intergenic
TATCGAGTTGGCGAAACATC+GGG 0.463450 5:-37486169 MsaT027211.1:CDS
ATCCTCTCAAGGAGAAGGCC+TGG 0.465771 5:-37484848 MsaT027211.1:CDS
GTCCTTAGATCCCTCATGCC+TGG 0.466194 5:-37485650 MsaT027211.1:CDS
GGAAACTTGAGAAAATAAAC+CGG 0.469347 5:+37485980 None:intergenic
TCATGAAGAAGCTCGAGTTT+CGG 0.469424 5:-37485840 MsaT027211.1:CDS
CAAAAGAGAAGAAGACTACT+AGG 0.471137 5:-37485070 MsaT027211.1:CDS
ATGTTGAGTGGTGATGGGTT+AGG 0.475552 5:-37485923 MsaT027211.1:CDS
GAAGCACAATCAAAACAAGT+TGG 0.486078 5:-37485046 MsaT027211.1:CDS
AAGAACTGTCTGTTCAATAG+TGG 0.499927 5:+37484760 None:intergenic
TGTGTTGAAGCTTGGTGGCA+TGG 0.500253 5:+37486261 None:intergenic
GCCTGGTTCATATGTGCAAA+GGG 0.500938 5:-37485633 MsaT027211.1:intron
CCAGATGAAGCTAGTTGAGA+TGG 0.511789 5:-37484960 MsaT027211.1:CDS
CTGTTCAATAGTGGTGATGT+TGG 0.513963 5:+37484769 None:intergenic
AATTTGATGTTGAGTGGTGA+TGG 0.521642 5:-37485929 MsaT027211.1:CDS
GTGTTGAAGCTTGGTGGCAT+GGG 0.524729 5:+37486262 None:intergenic
ATCGTCTTGATCAACTGTCC+AGG 0.524855 5:+37484830 None:intergenic
GTATCGAGTTGGCGAAACAT+CGG 0.525109 5:-37486170 MsaT027211.1:CDS
TGCAAGAGAACAACAAGAAG+AGG 0.529798 5:-37485778 MsaT027211.1:CDS
AGCTAGTGATACAAGGTTCA+CGG 0.534009 5:-37483933 MsaT027211.1:CDS
TCTTTATTCATTCAATGTCA+AGG 0.550164 5:-37484741 MsaT027211.1:intron
GTCCAGGCCTTCTCCTTGAG+AGG 0.550662 5:+37484846 None:intergenic
TTGAAGCTTGGTGGCATGGG+TGG 0.555219 5:+37486265 None:intergenic
GGTCATTTGGTAAAGTAGCT+GGG 0.556034 5:+37484981 None:intergenic
TGAAAATAACAACGGAATGG+TGG 0.565378 5:-37486146 MsaT027211.1:CDS
TGCCTGGTTCATATGTGCAA+AGG 0.568112 5:-37485634 MsaT027211.1:CDS
CTTCGATACCTGATTCTTCG+CGG 0.569325 5:+37486005 None:intergenic
TTAAAATCCTCTCAAGGAGA+AGG 0.575154 5:-37484853 MsaT027211.1:CDS
ATGCCTCAAACACAAACTTC+AGG 0.576898 5:-37486115 MsaT027211.1:CDS
GTTGGTGATGATGATGATCA+AGG 0.583272 5:+37486227 None:intergenic
TTCAGGTAGCTAGTGATACA+AGG 0.585732 5:-37483940 MsaT027211.1:intron
TAGTGGTGATGTTGGTGTGA+AGG 0.588630 5:+37484777 None:intergenic
TCAAGTGTGTTGAAGCTTGG+TGG 0.589540 5:+37486256 None:intergenic
CACATATGAACCAGGCATGA+GGG 0.590197 5:+37485640 None:intergenic
TGACTCACATAGCTGTTGAA+AGG 0.591781 5:-37485706 MsaT027211.1:CDS
ATTTGATGTTGAGTGGTGAT+GGG 0.593425 5:-37485928 MsaT027211.1:CDS
AGCACAATCAAAACAAGTTG+GGG 0.596948 5:-37485044 MsaT027211.1:CDS
GCACATATGAACCAGGCATG+AGG 0.603263 5:+37485639 None:intergenic
TAATCAAGTGTGTTGAAGCT+TGG 0.603690 5:+37486253 None:intergenic
CAATGATTAAAATCCTCTCA+AGG 0.606240 5:-37484859 MsaT027211.1:CDS
ATCAAGCTTCTATAATTGGG+GGG 0.607172 5:-37485144 MsaT027211.1:CDS
GAGGTTCAAATTAACAAGCG+TGG 0.607996 5:-37485888 MsaT027211.1:CDS
GACCAAAATTTGATGTTGAG+TGG 0.615025 5:-37485935 MsaT027211.1:CDS
TGATCAAGCTTCTATAATTG+GGG 0.615729 5:-37485146 MsaT027211.1:CDS
TAGCTGTTGAAAGGAATCGA+AGG 0.623409 5:-37485697 MsaT027211.1:CDS
AAAACTTAGTATCTGCTGAA+CGG 0.628856 5:+37483892 None:intergenic
ACCCTTTGCACATATGAACC+AGG 0.631479 5:+37485632 None:intergenic
GGGTGAAAATAACAACGGAA+TGG 0.632025 5:-37486149 MsaT027211.1:CDS
AGAATCAGGTATCGAAGACC+CGG 0.632243 5:-37485999 MsaT027211.1:CDS
CCTCAAACACAAACTTCAGG+AGG 0.637280 5:-37486112 MsaT027211.1:CDS
GATCAAGCTTCTATAATTGG+GGG 0.640085 5:-37485145 MsaT027211.1:CDS
TCAAGCTTCTATAATTGGGG+GGG 0.643949 5:-37485143 MsaT027211.1:CDS
TTGGTGATGATGATGATCAA+GGG 0.644829 5:+37486228 None:intergenic
AAAATTTGAGCAGAACAACA+TGG 0.657106 5:+37486303 None:intergenic
GAAACTTGAGAAAATAAACC+GGG 0.657788 5:+37485981 None:intergenic
GTTCAAATTAACAAGCGTGG+AGG 0.690801 5:-37485885 MsaT027211.1:CDS
GGTTAGGAGAAAATGACGAG+AGG 0.716694 5:-37485907 MsaT027211.1:CDS
ACATCGGGTGAAAATAACAA+CGG 0.731659 5:-37486154 MsaT027211.1:CDS
AGTTTCGGATGAGAACAACT+CGG 0.736695 5:-37485825 MsaT027211.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 37483868 37486323 37483868 ID=MsaG027211
Chr5 mRNA 37483868 37486323 37483868 ID=MsaT027211.1;Parent=MsaG027211
Chr5 exon 37483868 37483957 37483868 ID=MsaT027211.1.exon3;Parent=MsaT027211.1
Chr5 CDS 37483868 37483957 37483868 ID=cds.MsaT027211.1;Parent=MsaT027211.1
Chr5 exon 37484742 37485170 37484742 ID=MsaT027211.1.exon2;Parent=MsaT027211.1
Chr5 CDS 37484742 37485170 37484742 ID=cds.MsaT027211.1;Parent=MsaT027211.1
Chr5 exon 37485634 37486323 37485634 ID=MsaT027211.1.exon1;Parent=MsaT027211.1
Chr5 CDS 37485634 37486323 37485634 ID=cds.MsaT027211.1;Parent=MsaT027211.1
Gene Sequence

>MsaT027211.1

ATGTTGTTCTGCTCAAATTTTGAATCTTTGCAGGCACCACCCATGCCACCAAGCTTCAACACACTTGATTACTCCCTTGATCATCATCATCACCAACAACAACAACAACAACAATATGAACAGCTTTTGAAGTATCGAGTTGGCGAAACATCGGGTGAAAATAACAACGGAATGGTGGATAATTACATGCCTCAAACACAAACTTCAGGAGGTTTTTATGGTGCAAACTCTTTTGATAAAATGAGTTTTGCAGATGTGATGCAGTTTGCAGATTTTGGACCTAAATTAGCCTTAAACCGCGAAGAATCAGGTATCGAAGACCCGGTTTATTTTCTCAAGTTTCCTGTCTTAAACAATAAGATAGAAGACCAAAATTTGATGTTGAGTGGTGATGGGTTAGGAGAAAATGACGAGAGGTTCAAATTAACAAGCGTGGAGGATAAATCAAGAGATCAACAAGATCATGAAGAAGCTCGAGTTTCGGATGAGAACAACTCGGTGCAAGAGAAGAATTGTGCAGTAGTGCAAGAGAACAACAAGAAGAGGAAGAGACCAAGAACTGTTAAGACAAGTGAAGAAGTTGAGAGTCAACGTATGACTCACATAGCTGTTGAAAGGAATCGAAGGAAACAAATGAATGAGCATCTTCGTGTCCTTAGATCCCTCATGCCTGGTTCATATGTGCAAAGGGGTGATCAAGCTTCTATAATTGGGGGGGCTATAGAGTTTGTGAGGGAATTGGAACAACTTCTTCAGTGTCTAGAATCACAAAAGAGAAGAAGACTACTAGGAGAAGCACAATCAAAACAAGTTGGGGATTCATCTCTTGCATCAACACAACAACAGCCTCCATTTTTCCCAGCTACTTTACCAAATGACCAGATGAAGCTAGTTGAGATGGAAACTGGACTTCAAGAAGAAACTGCAGAGAGTAAGTCATGTTTGGCTGATGTAGAAGTGAAACTTCTAGGATTTGATGCAATGATTAAAATCCTCTCAAGGAGAAGGCCTGGACAGTTGATCAAGACGATTGCTGCTCTTGAAGATATGCAGCTCATTATCCTTCACACCAACATCACCACTATTGAACAGACAGTTCTTTATTCATTCAATGTCAAGGTAGCTAGTGATACAAGGTTCACGGCTGAAGATATAGCAAGCTCCGTTCAGCAGATACTAAGTTTTATTCATGCAAACACTAGCATGTGA

Protein sequence

>MsaT027211.1

MLFCSNFESLQAPPMPPSFNTLDYSLDHHHHQQQQQQQYEQLLKYRVGETSGENNNGMVDNYMPQTQTSGGFYGANSFDKMSFADVMQFADFGPKLALNREESGIEDPVYFLKFPVLNNKIEDQNLMLSGDGLGENDERFKLTSVEDKSRDQQDHEEARVSDENNSVQEKNCAVVQENNKKRKRPRTVKTSEEVESQRMTHIAVERNRRKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRLLGEAQSKQVGDSSLASTQQQPPFFPATLPNDQMKLVEMETGLQEETAESKSCLADVEVKLLGFDAMIKILSRRRPGQLIKTIAALEDMQLIILHTNITTIEQTVLYSFNVKVASDTRFTAEDIASSVQQILSFIHANTSM*