AlfalfaGEDB | MsaG027233

M. sativa ssp. caerulea / MsaG027233

KEGG: K10875
GO: GO:0000003 GO:0000018 GO:0000280 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006310 GO:0006311 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015616 GO:0016043 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022414 GO:0030491 GO:0030702 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0033170 GO:0034641 GO:0035822 GO:0035825 GO:0035861 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0060255 GO:0061806 GO:0061982 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
PFAM: PF00176.26

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027233	KEH43046.1	82.143	1120	121	8	1	1045	1	1116	0.0	1853

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027233	sp\|Q9M297\|CLSY1_ARATH	52.551	784	298	12	328	1045	480	1255	0.0	788
MsaG027233	sp\|Q9M297\|CLSY1_ARATH	35.909	220	124	6	4	208	2	219	2.44e-30	134

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027233	tr\|A0A072VN36\|A0A072VN36_MEDTR	82.143	1120	121	8	1	1045	1	1116	0.0	1853

TFs/TRs:

Gene ID	Type	Classification
MsaG027233	TR	SNF2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000766	MsaG027233	0.805160	2.579577e-49	3.925454e-47
MsaG002691	MsaG027233	0.807569	8.058211e-50	1.297817e-47
MsaG003789	MsaG027233	0.816560	9.016780e-52	1.813488e-49
MsaG004653	MsaG027233	0.802792	7.972955e-49	1.148493e-46
MsaG004776	MsaG027233	0.813009	5.472121e-51	1.006263e-48
MsaG005035	MsaG027233	0.809960	2.497283e-50	4.260529e-48
MsaG007096	MsaG027233	0.831965	2.236036e-55	6.849294e-53
MsaG008006	MsaG027233	0.807912	6.817831e-50	1.107201e-47
MsaG010943	MsaG027233	0.803127	6.801030e-49	9.873124e-47
MsaG011559	MsaG027233	0.810587	1.832103e-50	3.173802e-48
MsaG012466	MsaG027233	0.828268	1.767512e-54	4.869933e-52
MsaG042575	MsaG027233	0.802374	9.714722e-49	1.386128e-46
MsaG044632	MsaG027233	0.809515	3.109359e-50	5.248333e-48
MsaG044874	MsaG027233	0.802477	9.252135e-49	1.323218e-46
MsaG045627	MsaG027233	0.800420	2.430116e-48	3.317033e-46
MsaG045667	MsaG027233	0.811230	1.331497e-50	2.343141e-48
MsaG008728	MsaG027233	0.812009	9.031392e-51	1.620005e-48
MsaG014339	MsaG027233	0.805655	2.033798e-49	3.130711e-47
MsaG018949	MsaG027233	0.820443	1.199584e-52	2.669505e-50
MsaG027233	MsaG033256	0.820978	9.048643e-53	2.042354e-50
MsaG027233	MsaG035348	0.801539	1.439158e-48	2.014678e-46
MsaG027233	MsaG043656	0.805468	2.225515e-49	3.410837e-47
MsaG027233	MsaG044505	0.808939	4.126056e-50	6.868216e-48
MsaG012650	MsaG027233	0.809331	3.403685e-50	5.719153e-48
MsaG013189	MsaG027233	0.800380	2.476375e-48	3.377076e-46
MsaG013883	MsaG027233	0.808543	5.010376e-50	8.260977e-48
MsaG015776	MsaG027233	0.815342	1.681068e-51	3.277914e-49
MsaG015841	MsaG027233	0.813804	3.668370e-51	6.881447e-49
MsaG016180	MsaG027233	0.814348	2.785896e-51	5.297800e-49
MsaG017271	MsaG027233	0.826053	5.957936e-54	1.543095e-51
MsaG018252	MsaG027233	0.809066	3.877830e-50	6.474742e-48
MsaG020449	MsaG027233	0.805444	2.251300e-49	3.448469e-47
MsaG020766	MsaG027233	0.809337	3.394285e-50	5.704173e-48
MsaG021320	MsaG027233	0.806944	1.091597e-49	1.732177e-47
MsaG022548	MsaG027233	0.802605	8.707124e-49	1.248960e-46
MsaG025367	MsaG027233	0.815854	1.294665e-51	2.557375e-49
MsaG026280	MsaG027233	0.806224	1.545908e-49	2.411926e-47
MsaG028159	MsaG027233	0.807614	7.882953e-50	1.270980e-47
MsaG029742	MsaG027233	0.807571	8.049976e-50	1.296563e-47
MsaG030265	MsaG027233	0.800884	1.956837e-48	2.699033e-46
MsaG032764	MsaG027233	0.808009	6.503822e-50	1.058653e-47
MsaG033185	MsaG027233	0.803119	6.827886e-49	9.910231e-47
MsaG033565	MsaG027233	0.806807	1.166394e-49	1.844921e-47
MsaG034145	MsaG027233	0.829574	8.562169e-55	2.447977e-52
MsaG034653	MsaG027233	0.809898	2.575495e-50	4.387360e-48
MsaG035173	MsaG027233	0.823079	2.964795e-53	7.078604e-51
MsaG035170	MsaG027233	0.803046	7.067393e-49	1.024051e-46
MsaG035165	MsaG027233	0.800030	2.915030e-48	3.944028e-46
MsaG035278	MsaG027233	0.810252	2.162699e-50	3.716007e-48
MsaG037774	MsaG027233	0.801593	1.403186e-48	1.966706e-46
MsaG040364	MsaG027233	0.826729	4.119105e-54	1.087060e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027233	MtrunA17_Chr1g0191141	81.909	1100	120	8	21	1045	8	1103	0.0	1807
MsaG027233	MtrunA17_Chr7g0224051	59.236	1126	321	24	47	1044	1	1116	0.0	1217
MsaG027233	MtrunA17_Chr3g0114161	63.907	906	241	14	224	1044	343	1247	0.0	1085
MsaG027233	MtrunA17_Chr3g0114161	61.472	231	58	5	21	220	25	255	1.84e-73	266
MsaG027233	MtrunA17_Chr5g0437621	56.433	785	267	16	328	1044	526	1303	0.0	826
MsaG027233	MtrunA17_Chr5g0437621	42.412	257	118	8	5	234	4	257	4.33e-43	171
MsaG027233	MtrunA17_Chr7g0224031	64.602	226	78	2	601	824	17	242	1.73e-90	289
MsaG027233	MtrunA17_Chr7g0233031	31.111	720	396	21	376	1031	549	1232	6.73e-87	305
MsaG027233	MtrunA17_Chr7g0233021	31.136	713	392	19	376	1025	537	1213	2.41e-86	303
MsaG027233	MtrunA17_Chr7g0225791	30.312	706	408	20	376	1025	591	1268	8.44e-86	302
MsaG027233	MtrunA17_Chr4g0047081	30.506	731	402	22	376	1041	548	1237	1.45e-81	290
MsaG027233	MtrunA17_Chr7g0232971	30.176	739	399	23	376	1040	598	1293	1.11e-79	285
MsaG027233	MtrunA17_Chr2g0296421	28.488	681	378	21	362	1000	388	1001	1.75e-64	237
MsaG027233	MtrunA17_Chr7g0233001	31.608	541	310	16	528	1041	15	522	1.80e-64	227
MsaG027233	MtrunA17_Chr7g0232991	28.614	671	361	21	438	1031	17	646	1.70e-62	225
MsaG027233	MtrunA17_Chr8g0352361	28.378	666	383	20	371	1000	245	852	5.40e-62	228
MsaG027233	MtrunA17_Chr2g0320781	35.223	247	144	7	753	993	4	240	7.68e-37	139
MsaG027233	MtrunA17_Chr7g0224021	46.847	111	33	1	463	547	1	111	5.17e-25	101
MsaG027233	MtrunA17_Chr8g0384531	23.754	602	317	23	465	992	347	880	7.18e-24	108
MsaG027233	MtrunA17_Chr5g0393461	22.967	627	358	21	456	1030	163	716	1.30e-22	104
MsaG027233	MtrunA17_Chr5g0403921	24.959	605	338	24	473	1043	166	688	1.67e-19	94.4
MsaG027233	MtrunA17_Chr3g0137091	24.528	530	303	17	530	1034	342	799	1.95e-18	91.7
MsaG027233	MtrunA17_Chr1g0190381	32.895	152	92	3	863	1005	205	355	3.41e-17	86.3
MsaG027233	MtrunA17_Chr5g0442141	22.505	471	285	13	530	989	670	1071	1.05e-13	76.3
MsaG027233	MtrunA17_Chr3g0095091	23.207	474	278	15	530	989	624	1025	4.10e-13	74.3
MsaG027233	MtrunA17_Chr4g0040301	31.176	170	104	5	855	1017	389	552	7.43e-13	72.8
MsaG027233	MtrunA17_Chr3g0114111	88.235	34	4	0	998	1031	1	34	4.81e-12	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027233	AT3G42670.2	52.551	784	298	12	328	1045	480	1255	0.0	788
MsaG027233	AT3G42670.2	35.909	220	124	6	4	208	2	219	2.48e-31	134
MsaG027233	AT3G42670.3	52.551	784	298	12	328	1045	480	1255	0.0	788
MsaG027233	AT3G42670.3	35.909	220	124	6	4	208	2	219	2.48e-31	134
MsaG027233	AT3G42670.1	52.551	784	298	12	328	1045	480	1255	0.0	788
MsaG027233	AT3G42670.1	35.909	220	124	6	4	208	2	219	2.48e-31	134
MsaG027233	AT3G42670.4	52.672	786	296	13	328	1045	480	1257	0.0	786
MsaG027233	AT3G42670.4	35.909	220	124	6	4	208	2	219	2.38e-31	134
MsaG027233	AT5G20420.1	50.988	810	317	14	309	1045	459	1261	0.0	769
MsaG027233	AT5G20420.1	36.111	252	135	8	4	236	2	246	5.93e-36	148
MsaG027233	AT1G05490.1	29.930	715	415	20	376	1029	695	1384	3.07e-79	284
MsaG027233	AT3G24340.1	27.755	717	417	19	366	1033	450	1114	8.99e-69	251
MsaG027233	AT2G21450.3	27.637	673	386	20	375	1000	64	682	5.16e-58	214
MsaG027233	AT2G16390.2	29.378	691	368	23	362	1000	228	850	6.53e-58	216
MsaG027233	AT2G21450.1	27.637	673	386	20	375	1000	155	773	7.05e-58	215
MsaG027233	AT2G16390.1	29.378	691	368	23	362	1000	225	847	7.30e-58	216
MsaG027233	AT2G21450.2	27.637	673	386	20	375	1000	162	780	1.28e-57	214
MsaG027233	AT3G19210.2	24.092	523	314	18	527	1024	240	704	1.04e-22	105
MsaG027233	AT3G19210.1	24.571	525	311	19	527	1024	240	706	2.24e-22	104
MsaG027233	AT3G19210.3	24.571	525	311	19	527	1024	240	706	3.05e-22	103
MsaG027233	AT5G63950.2	24.219	384	239	14	638	992	495	855	3.43e-19	94.4
MsaG027233	AT5G63950.1	24.219	384	239	14	638	992	512	872	4.14e-19	94.0
MsaG027233	AT2G25170.1	25.370	473	270	14	528	983	321	727	2.56e-18	91.7
MsaG027233	AT2G25170.4	25.370	473	270	14	528	983	321	727	2.56e-18	91.7
MsaG027233	AT2G25170.3	25.370	473	270	14	528	983	347	753	3.19e-18	91.3
MsaG027233	AT2G25170.2	25.370	473	270	14	528	983	338	744	3.21e-18	91.3
MsaG027233	AT4G31900.3	23.312	459	269	12	530	983	276	656	3.22e-17	87.8
MsaG027233	AT4G31900.1	23.312	459	269	12	530	983	276	656	3.66e-17	87.8
MsaG027233	AT4G31900.2	23.312	459	269	12	530	983	235	615	6.60e-17	87.0
MsaG027233	AT1G08600.4	33.129	163	97	4	852	1005	1123	1282	2.36e-16	85.1
MsaG027233	AT1G08600.4	23.404	235	116	6	496	690	722	932	1.54e-11	69.3
MsaG027233	AT1G08600.3	33.129	163	97	4	852	1005	1123	1282	2.36e-16	85.1
MsaG027233	AT1G08600.3	23.404	235	116	6	496	690	722	932	1.54e-11	69.3
MsaG027233	AT1G08600.2	33.129	163	97	4	852	1005	1123	1282	2.36e-16	85.1
MsaG027233	AT1G08600.2	23.404	235	116	6	496	690	722	932	1.54e-11	69.3
MsaG027233	AT1G08600.1	33.129	163	97	4	852	1005	1102	1261	2.80e-16	85.1
MsaG027233	AT2G13370.1	22.614	482	286	15	526	992	671	1080	2.49e-15	82.0
MsaG027233	AT2G13370.2	22.614	482	286	15	526	992	671	1080	2.49e-15	82.0
MsaG027233	AT2G13370.3	22.614	482	286	15	526	992	671	1080	2.49e-15	82.0
MsaG027233	AT1G03750.1	30.481	187	115	7	847	1024	528	708	1.47e-13	75.9
MsaG027233	AT3G57300.1	28.571	196	122	6	827	1021	1187	1365	1.12e-11	70.1
MsaG027233	AT3G57300.4	28.571	196	122	6	827	1021	1187	1365	1.12e-11	70.1
MsaG027233	AT3G57300.2	28.571	196	122	6	827	1021	1220	1398	1.48e-11	69.7
MsaG027233	AT2G44980.1	24.311	399	246	14	639	1025	181	535	3.24e-11	68.2

Find 150 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTTGTTTCAAAACAATTT+TGG	0.098097	5:+38291519	MsaT027233.1:CDS
TGCTTGATACCATTAGAATT+TGG	0.209817	5:-38291240	None:intergenic
AGAACTTCGTGAATAAATTT+TGG	0.219280	5:-38291570	None:intergenic
AACTTTGATGTGGTACTTCT+AGG	0.221583	5:-38291444	None:intergenic
TATGGATTCGATAGCTTTAT+TGG	0.223781	5:+38289639	MsaT027233.1:CDS
CCAGGTAGAACCATTGATTT+TGG	0.226511	5:-38291213	None:intergenic
AAACTAGACGAAAACAAATT+TGG	0.228035	5:+38292011	MsaT027233.1:CDS
TATCGATTGGATGAACAAAT+TGG	0.242680	5:+38290358	MsaT027233.1:CDS
TTTATCTTCTGTTCTCATTA+AGG	0.252520	5:-38291343	None:intergenic
ACATCAAGTTATCTTCTTGA+TGG	0.278990	5:+38289946	MsaT027233.1:CDS
CCACAATCTAGGTTACTTTC+CGG	0.284693	5:+38289256	MsaT027233.1:CDS
CAAGATTGTGTATGAAGTTA+TGG	0.284889	5:+38289357	MsaT027233.1:CDS
CCAAAGAAAATGACAGTGTT+TGG	0.290902	5:+38290852	MsaT027233.1:CDS
TAATGGTATCAAGCATGATT+TGG	0.292292	5:+38291248	MsaT027233.1:CDS
TCGACCTGGTCAGGAGAAAA+TGG	0.292810	5:+38292394	MsaT027233.1:CDS
TGTTACCGCGTCATCTTTAA+AGG	0.296633	5:+38289306	MsaT027233.1:CDS
AGTTCTTCTGATACTCTTCC+TGG	0.348560	5:+38291789	MsaT027233.1:CDS
TTGAAGTTCAAAATATTCAT+AGG	0.349526	5:-38289403	None:intergenic
AAAGTTGAGGAAGTGCTTAA+TGG	0.350370	5:+38291461	MsaT027233.1:CDS
AGGTGGTTATATTGTTTCTT+CGG	0.350408	5:+38289558	MsaT027233.1:CDS
GCAGAAACTGCAAAAGAAAA+TGG	0.357505	5:+38291866	MsaT027233.1:CDS
GGTGGTTATATTGTTTCTTC+GGG	0.357997	5:+38289559	MsaT027233.1:CDS
TAACCCATGACAAGAACAAT+AGG	0.363802	5:-38291306	None:intergenic
TATTCATAGTCATTTGTTAT+CGG	0.364821	5:+38288755	MsaT027233.1:CDS
GAGTGGTCCAAAGGTTAATA+AGG	0.365464	5:+38289444	MsaT027233.1:CDS
CCTTCTTCATCACATGATAA+TGG	0.368117	5:-38289538	None:intergenic
GCTCTTCATTGCCACAATCT+AGG	0.368231	5:+38289245	MsaT027233.1:CDS
TATCTTTCTTCACTGATAGT+TGG	0.368473	5:-38289826	None:intergenic
TCAACTGATGCTACTGATCT+TGG	0.369974	5:+38290818	MsaT027233.1:CDS
TTCATAGGACCACTAATATT+AGG	0.372253	5:-38289388	None:intergenic
GTCATTTGTTATCGGACATT+CGG	0.377595	5:+38288763	MsaT027233.1:CDS
AAAATTTATTCACGAAGTTC+TGG	0.379765	5:+38291572	MsaT027233.1:CDS
AAGAAAATGGCGGGTTGTAC+TGG	0.380163	5:+38291879	MsaT027233.1:CDS
TTGTGCATGGATGAAATTTA+CGG	0.383973	5:-38288730	None:intergenic
AACAAATTTGGCTTGAGAAA+AGG	0.390540	5:+38292023	MsaT027233.1:CDS
AATAAGGAAAATCAGTTGAT+TGG	0.391273	5:+38289460	MsaT027233.1:CDS
AGTAAAATTCTCAATGTTGC+TGG	0.393610	5:-38291489	None:intergenic
CATGTGTAGAGTGTGGTCTT+TGG	0.395080	5:-38291108	None:intergenic
CAAGTCTAAACTTATTCATA+TGG	0.396817	5:+38292592	MsaT027233.1:CDS
CACAATCTAGGTTACTTTCC+GGG	0.397509	5:+38289257	MsaT027233.1:CDS
CCTGGAGTTCCAAATTCTAA+TGG	0.399610	5:+38291231	MsaT027233.1:CDS
GCTCAAAACAAATCTTACTT+TGG	0.400942	5:-38292048	None:intergenic
TCAATCAATGCTTGTGTTGA+AGG	0.404520	5:+38292284	MsaT027233.1:CDS
AAGAAAGAGGATAATGTGAT+TGG	0.408479	5:+38289110	MsaT027233.1:CDS
CACCCTATTGTTCTTGTCAT+GGG	0.409381	5:+38291303	MsaT027233.1:CDS
GATAAATTTGAAGATCGTTA+TGG	0.412152	5:+38292239	MsaT027233.1:CDS
AATCTTCTCAGCTTGCCATT+TGG	0.412165	5:-38292534	None:intergenic
CAAAGAAAATGACAGTGTTT+GGG	0.414944	5:+38290853	MsaT027233.1:CDS
AAAGAGATTCTGCAACTAAC+TGG	0.420585	5:+38292182	MsaT027233.1:CDS
TTGGTGTCGTCAAATGTCTC+TGG	0.420957	5:-38291194	None:intergenic
AAGATTGTGTATGAAGTTAT+GGG	0.424794	5:+38289358	MsaT027233.1:CDS
ATCATGTTCACATGTTTCCT+TGG	0.426716	5:-38290335	None:intergenic
ACGCGCGAGAAAATTCTTCT+TGG	0.431056	5:+38291659	MsaT027233.1:CDS
ACACCCTATTGTTCTTGTCA+TGG	0.438385	5:+38291302	MsaT027233.1:CDS
GACTGACATATGGAAAGAAA+TGG	0.444379	5:+38289912	MsaT027233.1:CDS
CATATGTCAGTCAAATCATC+AGG	0.444679	5:-38289901	None:intergenic
GTCTTTGGAGCTATGACTAA+TGG	0.446961	5:-38291093	None:intergenic
GGCAATGATCCTAATATTAG+TGG	0.453870	5:+38289379	MsaT027233.1:CDS
AAATGGCAAGCTGAGAAGAT+TGG	0.455864	5:+38292536	MsaT027233.1:CDS
GATAATGGAGACTCATTGTA+TGG	0.461722	5:-38289523	None:intergenic
TATCGATGATCATGAAGAAC+AGG	0.466478	5:+38289708	MsaT027233.1:CDS
CTGATGATTTGACTGACATA+TGG	0.466912	5:+38289902	MsaT027233.1:CDS
GCGGTTCTGTAAGGAGCATA+TGG	0.471624	5:+38289621	MsaT027233.1:CDS
CTTGTTGATGACTGGATCAA+TGG	0.472732	5:+38292430	MsaT027233.1:CDS
CCATTAGAATTTGGAACTCC+AGG	0.476634	5:-38291231	None:intergenic
GTATATCACTTGTTGATGAC+TGG	0.488667	5:+38292422	MsaT027233.1:CDS
TAAGAGAAGGCGGTTCTGTA+AGG	0.494575	5:+38289612	MsaT027233.1:CDS
TTTGACGACACCAAAATCAA+TGG	0.495623	5:+38291203	MsaT027233.1:CDS
TACTTCAACACTCTGTTTAG+CGG	0.497388	5:+38291546	MsaT027233.1:CDS
AATCTCACGTGTGATGAGTA+AGG	0.509552	5:+38292073	MsaT027233.1:CDS
GCAGGAGAAAGAAAACGTAT+TGG	0.511698	5:+38289855	MsaT027233.1:intron
GCGCATGGCTAAAGCATTGA+GGG	0.513248	5:+38291383	MsaT027233.1:CDS
TTCTCAGCTTGCCATTTGGA+AGG	0.514299	5:-38292530	None:intergenic
TTGTACTGGTTACTCTCTTG+AGG	0.516966	5:+38291893	MsaT027233.1:CDS
GAAGAACTACTTGGAAAGAG+TGG	0.517977	5:+38292468	MsaT027233.1:CDS
GTCTCTGGCTTCGACAGTTG+TGG	0.521572	5:-38291179	None:intergenic
AACTTCAAATCCTGTGATGA+TGG	0.524328	5:+38289418	MsaT027233.1:CDS
GAAACAATGCAGTGAACATA+CGG	0.535472	5:+38290760	MsaT027233.1:CDS
TATGATGATCAAGGTTCACT+TGG	0.537236	5:+38289068	MsaT027233.1:CDS
TTATCTTCTTGATGGAAATG+AGG	0.540645	5:+38289954	MsaT027233.1:CDS
AACCCATGACAAGAACAATA+GGG	0.541871	5:-38291305	None:intergenic
AATGTATATATTTGCAAACC+AGG	0.552272	5:-38291807	None:intergenic
GGAAGTAATTGATGCTTACA+TGG	0.555455	5:+38289771	MsaT027233.1:CDS
AGCATCAATTACTTCCTCGC+AGG	0.555873	5:-38289764	None:intergenic
CCAAAATCAATGGTTCTACC+TGG	0.556365	5:+38291213	MsaT027233.1:CDS
TTACGATAATTAGCATCGAA+TGG	0.560548	5:-38288174	None:intergenic
ACCACTCTTACCATCATCAC+AGG	0.562701	5:-38289428	None:intergenic
ATAAGTATAGAAGAACTACT+TGG	0.563508	5:+38292459	MsaT027233.1:CDS
TTAGATTCTAAGTACAGAAG+AGG	0.564016	5:+38291600	MsaT027233.1:CDS
TGACACAAATAAAGAATCCA+AGG	0.564564	5:+38290318	MsaT027233.1:CDS
AAGAACTACTTGGAAAGAGT+GGG	0.567137	5:+38292469	MsaT027233.1:CDS
GCGCGTGCCTCTAGTAAATG+AGG	0.568077	5:-38291642	None:intergenic
TCATCAAGTATCAAGAGTCC+AGG	0.569191	5:-38291411	None:intergenic
GGTTTCATATGTCATAAGAT+CGG	0.570324	5:+38289327	MsaT027233.1:CDS
AAGCAGTGTTGAATCACTCA+TGG	0.571957	5:+38288667	MsaT027233.1:CDS
AGATTGGAGATGATATACTC+AGG	0.572807	5:+38292552	MsaT027233.1:CDS
CCATTATCATGTGATGAAGA+AGG	0.574271	5:+38289538	MsaT027233.1:CDS
AGCGCATGGCTAAAGCATTG+AGG	0.574680	5:+38291382	MsaT027233.1:CDS
CACAATCTCGTGTTGTTCAG+TGG	0.577292	5:-38291844	None:intergenic
ATAGTTGGATCTTCCTCAGA+TGG	0.581528	5:-38289811	None:intergenic
AGGTCGAAAAGCGCGTGCAA+TGG	0.586494	5:-38292378	None:intergenic
TACTCATCACACGTGAGATT+AGG	0.587315	5:-38292070	None:intergenic
GATTGGAGATGATATACTCA+GGG	0.588398	5:+38292553	MsaT027233.1:CDS
TAGTTGAAGATCCTTCCAAA+TGG	0.588406	5:+38292519	MsaT027233.1:CDS
GAAGCGATACACTCCAAAGA+AGG	0.590117	5:+38289661	MsaT027233.1:CDS
AAAGCATTGAGGGAAAGTCC+TGG	0.590693	5:+38291393	MsaT027233.1:CDS
GCACTTCCTCAACTTTGATG+TGG	0.590805	5:-38291454	None:intergenic
ACGATCAAGAAAAGCTACTT+CGG	0.591655	5:+38288788	MsaT027233.1:CDS
CTTCAACGAAATAACTGTGA+AGG	0.592014	5:+38289161	MsaT027233.1:CDS
AAACTGCAAAAGAAAATGGC+GGG	0.594652	5:+38291870	MsaT027233.1:CDS
AGATGATATACTCAGGGAGA+TGG	0.595348	5:+38292559	MsaT027233.1:CDS
ACTCAGGGAGATGGTTGAAG+AGG	0.596695	5:+38292568	MsaT027233.1:CDS
GTATACAAAAGATCAAAAGC+TGG	0.604364	5:+38291274	MsaT027233.1:CDS
TTGGATCTTCCTCAGATGGT+AGG	0.604569	5:-38289807	None:intergenic
CCGGAAAGTAACCTAGATTG+TGG	0.605716	5:-38289256	None:intergenic
TGGACTCTTGATACTTGATG+AGG	0.608858	5:+38291413	MsaT027233.1:CDS
GGAATATACTGCCGTACATG+CGG	0.610029	5:+38290379	MsaT027233.1:CDS
CGTATTGGAAAGCGGTGGTG+AGG	0.610073	5:+38289870	MsaT027233.1:CDS
AGATGACGCGGTAACAAACC+AGG	0.611437	5:-38289299	None:intergenic
CAAAGACCACACTCTACACA+TGG	0.613075	5:+38291109	MsaT027233.1:CDS
GCACGAAGAATCTTAAGAGA+AGG	0.613730	5:+38289599	MsaT027233.1:CDS
CACTCGGGTCAATACACCCA+TGG	0.615844	5:+38291931	MsaT027233.1:CDS
CAGAAGAGGCAAACTAGAGG+AGG	0.618526	5:+38291614	MsaT027233.1:CDS
AAAATCACACAGAAAGCGCA+TGG	0.619203	5:+38291368	MsaT027233.1:CDS
TGGATAAGATACACCGACAT+GGG	0.619325	5:-38291159	None:intergenic
GTCACAACCTTGCACCCGTG+AGG	0.623609	5:+38292114	MsaT027233.1:CDS
GAACATTTATTATATTTGTG+AGG	0.623911	5:-38289023	None:intergenic
AAAATGGAATATTCCCATGT+CGG	0.627043	5:+38291146	MsaT027233.1:CDS
ATGTGAACATGATTATCGAT+TGG	0.633134	5:+38290345	MsaT027233.1:CDS
TGATGGTAAGAGTGGTCCAA+AGG	0.634249	5:+38289435	MsaT027233.1:CDS
GTACAGAAGAGGCAAACTAG+AGG	0.636952	5:+38291611	MsaT027233.1:CDS
GGACTCTTGATACTTGATGA+GGG	0.637751	5:+38291414	MsaT027233.1:CDS
AATTAACTCAAATATTGATG+CGG	0.638750	5:+38291698	MsaT027233.1:CDS
CATGAAGAACAGGATCATAG+AGG	0.639495	5:+38289718	MsaT027233.1:CDS
GAAACTGCAAAAGAAAATGG+CGG	0.646622	5:+38291869	MsaT027233.1:CDS
CGAAGAATCTTAAGAGAAGG+CGG	0.646747	5:+38289602	MsaT027233.1:CDS
TCCTGTGATGATGGTAAGAG+TGG	0.654377	5:+38289427	MsaT027233.1:CDS
GAAGTACCACATCAAAGTTG+AGG	0.659873	5:+38291448	MsaT027233.1:CDS
GAATCACTCATGGCAACCTG+TGG	0.660741	5:+38288677	MsaT027233.1:CDS
TAGATTGTGGCAATGAAGAG+CGG	0.677170	5:-38289243	None:intergenic
GTGGATAAGATACACCGACA+TGG	0.681468	5:-38291160	None:intergenic
AAAGAAAACGTATTGGAAAG+CGG	0.682656	5:+38289862	MsaT027233.1:intron
TTATCATGTGATGAAGAAGG+TGG	0.694141	5:+38289541	MsaT027233.1:CDS
GAAGAACAGGATCATAGAGG+CGG	0.697410	5:+38289721	MsaT027233.1:CDS
GGAAGCGCCTCATTTACTAG+AGG	0.697989	5:+38291635	MsaT027233.1:CDS
TGAAACCTTTAAAGATGACG+CGG	0.702218	5:-38289311	None:intergenic
GCAGGGTGCAAATGATGACG+AGG	0.707116	5:+38290288	MsaT027233.1:intron
GTTGATGACTGGATCAATGG+AGG	0.715451	5:+38292433	MsaT027233.1:CDS
GAAAACGTATTGGAAAGCGG+TGG	0.722789	5:+38289865	MsaT027233.1:intron
GTTAAATGCTGATACCTGCG+AGG	0.758695	5:+38289750	MsaT027233.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	38288135	38292646	38288135	ID=MsaG027233
Chr5	mRNA	38288135	38292646	38288135	ID=MsaT027233.1;Parent=MsaG027233
Chr5	exon	38288135	38288192	38288135	ID=MsaT027233.1.exon1;Parent=MsaT027233.1
Chr5	CDS	38288135	38288192	38288135	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38288661	38288860	38288661	ID=MsaT027233.1.exon2;Parent=MsaT027233.1
Chr5	CDS	38288661	38288860	38288661	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38288984	38289186	38288984	ID=MsaT027233.1.exon3;Parent=MsaT027233.1
Chr5	CDS	38288984	38289186	38288984	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38289225	38289835	38289225	ID=MsaT027233.1.exon4;Parent=MsaT027233.1
Chr5	CDS	38289225	38289835	38289225	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38289866	38289975	38289866	ID=MsaT027233.1.exon5;Parent=MsaT027233.1
Chr5	CDS	38289866	38289975	38289866	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38290292	38290441	38290292	ID=MsaT027233.1.exon6;Parent=MsaT027233.1
Chr5	CDS	38290292	38290441	38290292	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38290758	38291001	38290758	ID=MsaT027233.1.exon7;Parent=MsaT027233.1
Chr5	CDS	38290758	38291001	38290758	ID=cds.MsaT027233.1;Parent=MsaT027233.1
Chr5	exon	38291085	38292646	38291085	ID=MsaT027233.1.exon8;Parent=MsaT027233.1
Chr5	CDS	38291085	38292646	38291085	ID=cds.MsaT027233.1;Parent=MsaT027233.1

Gene Sequence

>MsaT027233.1

ATGGCGCCTGAAAAAAGTTATTTGCATCAAACGAAACATCCATTCGATGCTAATTATCCATTTGAAGCAGTGTTGAATCACTCATGGCAACCTGTGGAACTCATAAAAATTGAGTCTGGGAAAACTACCGTAAATTTCATCCATGCACAAAATATTCATAGTCATTTGTTATCGGACATTCGGATACGATCAAGAAAAGCTACTTCGGTTGATTGCTCCAGTTTTGAATACAATAAAAATTCACGTGAATTTAGCCCAATGTCGACTGATGCTAGAGTGAATTGCATACACAAAAAACCTCACAAATATAATAAATGTTCGTGTCGATTTCATGTAAAGTTTTATGATGATCAAGGTTCACTTGGCACAGAAATAAAAACTCTGAAGAAAGAGGATAATGTGATTGGAATTGAGAAAATTTTCATCCTCCAAAAACTTCAACGAAATAACTGTGAAGGATCGTGGGATTCATCGGAAGACCGCTCTTCATTGCCACAATCTAGGTTACTTTCCGGGAAGTTTTTGTCTGATCTTTCCTGGTTTGTTACCGCGTCATCTTTAAAGGTTTCATATGTCATAAGATCGGTTCAAAACAAGATTGTGTATGAAGTTATGGGCAATGATCCTAATATTAGTGGTCCTATGAATATTTTGAACTTCAAATCCTGTGATGATGGTAAGAGTGGTCCAAAGGTTAATAAGGAAAATCAGTTGATTGGTGCTAAAGAAAAGAACGTTGTTGATGTGTTAAATCATGAGCCATACAATGAGTCTCCATTATCATGTGATGAAGAAGGTGGTTATATTGTTTCTTCGGGACATGTTAACAATTTCTGCACGAAGAATCTTAAGAGAAGGCGGTTCTGTAAGGAGCATATGGATTCGATAGCTTTATTGGAAGCGATACACTCCAAAGAAGGTGTTCAAAAGAAAACGACAAACAGTATCGATGATCATGAAGAACAGGATCATAGAGGCGGTAGAACGTTAAATGCTGATACCTGCGAGGAAGTAATTGATGCTTACATGGATAATTTTGATAGCCTACCATCTGAGGAAGATCCAACTATCAAAAACGTATTGGAAAGCGGTGGTGAGGTAGAAAATCCTGATGATTTGACTGACATATGGAAAGAAATGGATACAACTTTGACATCAAGTTATCTTCTTGATGGAAATGAGGGTGCAAATGATGACGAGGTTTTAGCTGACACAAATAAAGAATCCAAGGAAACATGTGAACATGATTATCGATTGGATGAACAAATTGGAATATACTGCCGTACATGCGGCTTTGTGAAAACTGAGAAACGATACATTTCTGAACCCATTAAGAAACAATGCAGTGAACATACGGAACAAGTTGATGAAGATGATAATAATGATATGTTTTCAACTGATGCTACTGATCTTGGTGAACGAATATCCAAAGAAAATGACAGTGTTTGGGAATTGATTCCTGAACTCAAAGAAAACATGCATTCACACCAGAAAAAAGCTTTTCAATTTCTTTGGAAAAACATTGCAGGGTCTATGGAGCAATCACACATGAAAGAAAAATCTGACACAACTGGTGAGAAAATACCATTAGTCATAGCTCCAAAGACCACACTCTACACATGGCAAAAAGAGTTCGAAAAATGGAATATTCCCATGTCGGTGTATCTTATCCACAACTGTCGAAGCCAGAGACATTTGACGACACCAAAATCAATGGTTCTACCTGGAGTTCCAAATTCTAATGGTATCAAGCATGATTTGGATTGTATACAAAAGATCAAAAGCTGGAATTCACACCCTATTGTTCTTGTCATGGGTTATTCTTCATTTTTAGCCTTAATGAGAACAGAAGATAAAAAAAAATCACACAGAAAGCGCATGGCTAAAGCATTGAGGGAAAGTCCTGGACTCTTGATACTTGATGAGGGACACAATCCTAGAAGTACCACATCAAAGTTGAGGAAGTGCTTAATGGATCTACCAGCAACATTGAGAATTTTACTTTCTGGCACTTTGTTTCAAAACAATTTTGGGGAATACTTCAACACTCTGTTTAGCGGACCAAAATTTATTCACGAAGTTCTGGAAGAGTTAGATTCTAAGTACAGAAGAGGCAAACTAGAGGAGGAAGCGCCTCATTTACTAGAGGCACGCGCGAGAAAATTCTTCTTGGAGAACATTGAAAAGAAAATTAACTCAAATATTGATGCGGAAAAAATGAAGGGTGTAGATGTGCTTCGAAAAATCAAAAATGGTTTTATAGATGTTTATGACAGTGCAAGTTCTTCTGATACTCTTCCTGGTTTGCAAATATATACATTGCTCGTGAATGCATCCACTGAACAACACGAGATTGTGCAGAAACTGCAAAAGAAAATGGCGGGTTGTACTGGTTACTCTCTTGAGGTTGAGCTTTTGATAACACTCGGGTCAATACACCCATGGTTGATCAAAACAGCAGAAACATGTGCAGCTAAGTTTTTCTCCGGTGAAGAGTTGAAAAAACTAGACGAAAACAAATTTGGCTTGAGAAAAGGTTCCAAAGTAAGATTTGTTTTGAGCCTAATCTCACGTGTGATGAGTAAGGAAAAGATACTTATCTTCTGTCACAACCTTGCACCCGTGAGGTTTTTAGTAGAGTTATTTGAGAATCATTTTCAGTGGAAAAATGGTAAAGAGATTCTGCAACTAACTGGTGAACAAGACTTCTTTGAAAGAACAAATGTGATAGATAAATTTGAAGATCGTTATGGCGATTCAAAGATAATTCTTGCTTCAATCAATGCTTGTGTTGAAGGTATCAGTTTAACAGTAGCTTCAAGAGTGATATTTTTGGATTCTGAGTGGAATCCTTCAAAAACAAAGGAAGCCATTGCACGCGCTTTTCGACCTGGTCAGGAGAAAATGGTTTATGTATATCACTTGTTGATGACTGGATCAATGGAGGAAGATAAGTATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGCATGATTTTCAGTGAGGAGCTAGTTGAAGATCCTTCCAAATGGCAAGCTGAGAAGATTGGAGATGATATACTCAGGGAGATGGTTGAAGAGGACAAGTCTAAACTTATTCATATGGTTATGAAGAATGAAAAGACTTCAACAAGCTAG

Protein sequence

>MsaT027233.1

MAPEKSYLHQTKHPFDANYPFEAVLNHSWQPVELIKIESGKTTVNFIHAQNIHSHLLSDIRIRSRKATSVDCSSFEYNKNSREFSPMSTDARVNCIHKKPHKYNKCSCRFHVKFYDDQGSLGTEIKTLKKEDNVIGIEKIFILQKLQRNNCEGSWDSSEDRSSLPQSRLLSGKFLSDLSWFVTASSLKVSYVIRSVQNKIVYEVMGNDPNISGPMNILNFKSCDDGKSGPKVNKENQLIGAKEKNVVDVLNHEPYNESPLSCDEEGGYIVSSGHVNNFCTKNLKRRRFCKEHMDSIALLEAIHSKEGVQKKTTNSIDDHEEQDHRGGRTLNADTCEEVIDAYMDNFDSLPSEEDPTIKNVLESGGEVENPDDLTDIWKEMDTTLTSSYLLDGNEGANDDEVLADTNKESKETCEHDYRLDEQIGIYCRTCGFVKTEKRYISEPIKKQCSEHTEQVDEDDNNDMFSTDATDLGERISKENDSVWELIPELKENMHSHQKKAFQFLWKNIAGSMEQSHMKEKSDTTGEKIPLVIAPKTTLYTWQKEFEKWNIPMSVYLIHNCRSQRHLTTPKSMVLPGVPNSNGIKHDLDCIQKIKSWNSHPIVLVMGYSSFLALMRTEDKKKSHRKRMAKALRESPGLLILDEGHNPRSTTSKLRKCLMDLPATLRILLSGTLFQNNFGEYFNTLFSGPKFIHEVLEELDSKYRRGKLEEEAPHLLEARARKFFLENIEKKINSNIDAEKMKGVDVLRKIKNGFIDVYDSASSSDTLPGLQIYTLLVNASTEQHEIVQKLQKKMAGCTGYSLEVELLITLGSIHPWLIKTAETCAAKFFSGEELKKLDENKFGLRKGSKVRFVLSLISRVMSKEKILIFCHNLAPVRFLVELFENHFQWKNGKEILQLTGEQDFFERTNVIDKFEDRYGDSKIILASINACVEGISLTVASRVIFLDSEWNPSKTKEAIARAFRPGQEKMVYVYHLLMTGSMEEDKYRRTTWKEWVSCMIFSEELVEDPSKWQAEKIGDDILREMVEEDKSKLIHMVMKNEKTSTS*