AlfalfaGEDB | MsaG027390

M. sativa ssp. caerulea / MsaG027390

KEGG: K15601
GO: GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005102 GO:0005488 GO:0005506 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006081 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0008214 GO:0009058 GO:0009719 GO:0009725 GO:0009755 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0016999 GO:0017000 GO:0017144 GO:0019219 GO:0019222 GO:0019538 GO:0023052 GO:0030518 GO:0030521 GO:0030522 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0032870 GO:0033169 GO:0033993 GO:0035257 GO:0035258 GO:0036211 GO:0042221 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043401 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0046184 GO:0046292 GO:0046293 GO:0046872 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0051427 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0070076 GO:0070887 GO:0070988 GO:0071310 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF02373.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027390	XP_019441694.1	57.187	654	201	11	1	580	17	665	0.0	669

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027390	sp\|Q9SSE9\|JMJ25_ARATH	57.468	308	96	3	311	586	591	895	3.16e-107	348
MsaG027390	sp\|Q9SSE9\|JMJ25_ARATH	43.147	197	110	2	1	197	154	348	6.44e-46	177

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027390	tr\|A0A2Z6LK29\|A0A2Z6LK29_TRISU	48.601	679	234	13	2	586	225	882	0.0	572

TFs/TRs:

Gene ID	Type	Classification
MsaG027390	TR	Jumonji

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000308	MsaG027390	0.817448	5.709960e-52	1.174979e-49
MsaG000942	MsaG027390	0.846649	3.582566e-59	1.733705e-56
MsaG001009	MsaG027390	0.803099	6.892530e-49	9.999462e-47
MsaG002718	MsaG027390	0.812425	7.337503e-51	1.329776e-48
MsaG003768	MsaG027390	0.816520	9.203187e-52	1.849056e-49
MsaG004279	MsaG027390	0.814810	2.204040e-51	4.240218e-49
MsaG004479	MsaG027390	0.817161	6.617906e-52	1.351838e-49
MsaG004527	MsaG027390	0.806586	1.298233e-49	2.042873e-47
MsaG004998	MsaG027390	0.826362	5.034054e-54	1.315019e-51
MsaG005584	MsaG027390	0.819976	1.532851e-52	3.369161e-50
MsaG006334	MsaG027390	0.819742	1.732561e-52	3.784302e-50
MsaG007109	MsaG027390	0.802610	8.688704e-49	1.246437e-46
MsaG008201	MsaG027390	0.808751	4.526099e-50	7.500226e-48
MsaG009059	MsaG027390	0.823328	2.595661e-53	6.239147e-51
MsaG009456	MsaG027390	0.805234	2.490220e-49	3.795895e-47
MsaG009722	MsaG027390	0.828277	1.759019e-54	4.847662e-52
MsaG010619	MsaG027390	0.853160	5.515181e-61	3.336940e-58
MsaG012409	MsaG027390	0.807584	7.998312e-50	1.288649e-47
MsaG012448	MsaG027390	0.824460	1.412909e-53	3.502220e-51
MsaG014376	MsaG027390	0.819314	2.166827e-52	4.680342e-50
MsaG014905	MsaG027390	0.808011	6.495710e-50	1.057397e-47
MsaG015092	MsaG027390	0.815691	1.406906e-51	2.767700e-49
MsaG016307	MsaG027390	0.806627	1.272319e-49	2.004061e-47
MsaG016651	MsaG027390	0.804579	3.407163e-49	5.115227e-47
MsaG016826	MsaG027390	0.856284	6.928736e-62	4.690272e-59
MsaG017543	MsaG027390	0.810451	1.959648e-50	3.383612e-48
MsaG017689	MsaG027390	0.833298	1.047641e-55	3.336266e-53
MsaG018367	MsaG027390	0.811956	9.275682e-51	1.661681e-48
MsaG018913	MsaG027390	0.841748	7.311116e-58	3.015811e-55
MsaG019303	MsaG027390	0.833862	7.589872e-56	2.457268e-53
MsaG019773	MsaG027390	0.832458	1.690825e-55	5.253639e-53
MsaG020393	MsaG027390	0.811220	1.338262e-50	2.354471e-48
MsaG020616	MsaG027390	0.828619	1.455527e-54	4.050101e-52
MsaG020814	MsaG027390	0.804175	4.133043e-49	6.147237e-47
MsaG021997	MsaG027390	0.801985	1.166695e-48	1.649902e-46
MsaG022787	MsaG027390	0.800899	1.942543e-48	2.680242e-46
MsaG023503	MsaG027390	0.823586	2.260477e-53	5.471590e-51
MsaG023609	MsaG027390	0.835452	3.035345e-56	1.030585e-53
MsaG025958	MsaG027390	0.848429	1.167579e-59	5.997855e-57
MsaG027390	MsaG027398	0.956113	2.166427e-113	8.124206e-108
MsaG027390	MsaG028951	0.814465	2.625565e-51	5.007450e-49
MsaG027390	MsaG029838	0.813192	4.991432e-51	9.220744e-49
MsaG027390	MsaG029868	0.817979	4.338249e-52	9.050080e-50
MsaG027390	MsaG030327	0.800068	2.864330e-48	3.878705e-46
MsaG027390	MsaG030359	0.818674	3.024772e-52	6.424421e-50
MsaG027390	MsaG030546	0.817528	5.477573e-52	1.129470e-49
MsaG027390	MsaG030955	0.869463	6.163982e-66	6.984148e-63
MsaG027390	MsaG030986	0.814223	2.967091e-51	5.624695e-49
MsaG027390	MsaG032786	0.815615	1.463002e-51	2.872516e-49
MsaG027390	MsaG033694	0.805217	2.510144e-49	3.824774e-47
MsaG027390	MsaG034253	0.807862	6.988439e-50	1.133520e-47
MsaG027390	MsaG034875	0.813390	4.518003e-51	8.387690e-49
MsaG027390	MsaG036047	0.827376	2.889155e-54	7.763714e-52
MsaG027390	MsaG036952	0.803614	5.398142e-49	7.924921e-47
MsaG027390	MsaG039679	0.824153	1.666744e-53	4.096789e-51
MsaG027390	MsaG040045	0.809221	3.593973e-50	6.022868e-48
MsaG027390	MsaG041574	0.802319	9.969976e-49	1.420748e-46
MsaG027390	MsaG042103	0.807596	7.953698e-50	1.281803e-47
MsaG027390	MsaG043987	0.805024	2.753427e-49	4.176820e-47
MsaG027390	MsaG044218	0.800959	1.888703e-48	2.609476e-46
MsaG027390	MsaG044885	0.804986	2.804465e-49	4.250313e-47
MsaG027390	MsaG045345	0.824547	1.348105e-53	3.349512e-51
MsaG027390	MsaG045974	0.818404	3.479532e-52	7.338692e-50
MsaG027390	MsaG046554	0.818899	2.690774e-52	5.748742e-50
MsaG027390	MsaG047014	0.845581	6.973422e-59	3.257056e-56
MsaG027390	MsaG001552	0.804951	2.851165e-49	4.317706e-47
MsaG027390	MsaG022374	0.861051	2.652206e-63	2.146317e-60
MsaG027390	MsaG018378	0.820675	1.061829e-52	2.377263e-50
MsaG027390	MsaG019831	0.803496	5.708227e-49	8.357445e-47
MsaG027390	MsaG023443	0.862664	8.560048e-64	7.372277e-61
MsaG027390	MsaG026431	0.829569	8.584686e-55	2.454037e-52
MsaG027390	MsaG026536	0.837395	9.778870e-57	3.520633e-54
MsaG027390	MsaG029571	0.806492	1.358174e-49	2.132478e-47
MsaG027390	MsaG032663	0.807950	6.694547e-50	1.088131e-47
MsaG027390	MsaG030315	0.819133	2.382131e-52	5.120899e-50
MsaG027390	MsaG039132	0.830300	5.707571e-55	1.666050e-52
MsaG027390	MsaG042291	0.826106	5.787584e-54	1.501166e-51
MsaG027390	MsaG042301	0.800180	2.718140e-48	3.689932e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027390	MtrunA17_Chr5g0426961	90.064	312	31	0	1	312	139	450	0.0	567
MsaG027390	MtrunA17_Chr5g0426961	85.621	306	16	2	309	587	533	837	1.05e-178	526
MsaG027390	MtrunA17_Chr1g0211711	73.438	320	41	3	311	586	733	1052	2.71e-153	467
MsaG027390	MtrunA17_Chr1g0211711	69.085	317	93	4	1	316	342	654	7.61e-146	448
MsaG027390	MtrunA17_Chr1g0183311	66.862	341	43	2	316	586	543	883	2.89e-148	449
MsaG027390	MtrunA17_Chr1g0183311	66.561	314	103	2	2	314	144	456	7.67e-127	394
MsaG027390	MtrunA17_Chr1g0211661	62.821	312	115	1	1	312	224	534	8.42e-126	391
MsaG027390	MtrunA17_Chr1g0211661	64.113	248	56	2	311	525	618	865	2.74e-97	316
MsaG027390	MtrunA17_Chr1g0211691	62.687	335	118	4	1	331	341	672	9.70e-126	392
MsaG027390	MtrunA17_Chr1g0211691	63.942	208	30	3	311	473	738	945	1.28e-72	250
MsaG027390	MtrunA17_Chr5g0420361	62.848	323	59	5	311	575	469	788	6.95e-124	384
MsaG027390	MtrunA17_Chr5g0420361	55.556	261	111	3	73	331	145	402	6.54e-82	273
MsaG027390	MtrunA17_Chr2g0289121	59.406	303	84	3	317	582	519	819	1.37e-110	350
MsaG027390	MtrunA17_Chr2g0289121	40.467	257	138	6	2	258	156	397	2.71e-43	166
MsaG027390	MtrunA17_Chr6g0472441	55.460	348	78	7	287	575	381	710	3.22e-109	343
MsaG027390	MtrunA17_Chr6g0472441	54.373	263	116	3	73	331	87	349	5.03e-80	266
MsaG027390	MtrunA17_Chr4g0075261	53.509	342	89	3	311	582	523	864	1.96e-108	345
MsaG027390	MtrunA17_Chr4g0075261	37.931	261	146	7	2	260	168	414	4.68e-45	171
MsaG027390	MtrunA17_Chr2g0289031	58.362	293	93	2	317	582	103	393	8.74e-108	329
MsaG027390	MtrunA17_Chr5g0448301	60.550	218	53	2	311	495	414	631	5.72e-78	259
MsaG027390	MtrunA17_Chr5g0448301	50.446	224	91	3	89	312	126	329	5.36e-54	194
MsaG027390	MtrunA17_Chr1g0211701	83.478	115	19	0	471	585	4	118	2.12e-64	208
MsaG027390	MtrunA17_Chr1g0192011	36.176	340	141	4	315	582	625	960	9.41e-63	223
MsaG027390	MtrunA17_Chr1g0192011	31.310	313	172	9	2	313	267	537	1.51e-36	145
MsaG027390	MtrunA17_Chr7g0275771	62.130	169	61	1	417	582	756	924	1.63e-62	222
MsaG027390	MtrunA17_Chr7g0275771	37.423	326	177	8	1	310	139	453	4.89e-57	206
MsaG027390	MtrunA17_Chr7g0275771	45.333	150	46	2	317	439	547	687	1.18e-26	115
MsaG027390	MtrunA17_Chr1g0147021	69.737	152	43	1	438	586	1551	1702	3.60e-60	217
MsaG027390	MtrunA17_Chr1g0147021	35.647	317	164	9	2	312	870	1152	5.81e-45	172
MsaG027390	MtrunA17_Chr1g0147021	51.562	128	34	2	315	415	1240	1366	4.33e-24	107
MsaG027390	MtrunA17_Chr2g0289091	61.905	147	46	3	411	547	274	420	4.26e-51	181
MsaG027390	MtrunA17_Chr2g0289091	47.967	123	36	2	317	412	42	163	1.38e-25	109
MsaG027390	MtrunA17_Chr2g0281051	30.728	371	165	6	304	586	592	958	3.58e-46	175
MsaG027390	MtrunA17_Chr2g0281051	29.907	321	210	7	2	316	207	518	1.94e-31	130
MsaG027390	MtrunA17_Chr2g0289041	41.148	209	114	4	24	232	2	201	9.13e-45	157
MsaG027390	MtrunA17_Chr4g0048531	53.378	148	65	2	437	581	1132	1278	9.75e-44	167
MsaG027390	MtrunA17_Chr4g0048531	38.323	167	97	3	2	163	260	425	3.49e-29	123
MsaG027390	MtrunA17_Chr4g0048531	36.220	127	51	3	306	405	893	1016	1.35e-12	71.2
MsaG027390	MtrunA17_Chr8g0361391	70.526	95	27	1	431	525	44	137	2.48e-41	147
MsaG027390	MtrunA17_Chr7g0231061	58.333	132	28	2	318	422	45	176	5.36e-39	142
MsaG027390	MtrunA17_Chr8g0364421	39.130	207	94	4	111	316	3	178	8.87e-34	128
MsaG027390	MtrunA17_Chr8g0364411	53.982	113	51	1	2	114	33	144	1.13e-32	122
MsaG027390	MtrunA17_Chr1g0211671	75.806	62	15	0	524	585	15	76	1.36e-26	105
MsaG027390	MtrunA17_Chr8g0340381	51.163	129	25	2	319	424	36	149	1.35e-21	92.8
MsaG027390	MtrunA17_Chr8g0361381	50.000	90	18	1	317	379	119	208	6.60e-21	91.7
MsaG027390	MtrunA17_Chr3g0119761	61.667	60	23	0	365	424	55	114	1.73e-13	67.8
MsaG027390	MtrunA17_Chr2g0289101	32.558	129	81	3	135	262	8	131	2.00e-13	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027390	AT1G11950.1	57.333	300	101	1	311	583	570	869	1.16e-112	357
MsaG027390	AT1G11950.1	35.256	312	179	6	1	310	192	482	7.62e-47	177
MsaG027390	AT1G11950.3	57.333	300	101	1	311	583	570	869	1.16e-112	357
MsaG027390	AT1G11950.3	35.256	312	179	6	1	310	192	482	7.62e-47	177
MsaG027390	AT3G07610.1	57.468	308	96	3	311	586	591	895	3.22e-108	348
MsaG027390	AT3G07610.1	43.147	197	110	2	1	197	154	348	6.55e-47	177
MsaG027390	AT1G62310.1	58.076	291	87	3	311	566	588	878	2.68e-104	335
MsaG027390	AT1G62310.1	33.227	313	190	6	1	312	208	502	6.75e-45	171
MsaG027390	AT3G07610.3	54.434	327	98	4	311	586	591	917	7.77e-102	332
MsaG027390	AT3G07610.3	43.147	197	110	2	1	197	154	348	5.71e-47	177
MsaG027390	AT1G11950.2	53.879	232	80	1	311	515	570	801	1.17e-74	254
MsaG027390	AT1G11950.2	34.727	311	182	5	1	310	192	482	4.50e-47	177
MsaG027390	AT4G00990.1	61.963	163	57	2	419	581	671	828	1.93e-58	210
MsaG027390	AT4G00990.1	32.698	315	185	10	2	312	80	371	1.68e-34	140
MsaG027390	AT4G00990.1	41.875	160	63	3	311	441	458	616	3.66e-27	117
MsaG027390	AT1G09060.2	34.242	330	143	8	318	582	570	890	9.21e-49	182
MsaG027390	AT1G09060.2	30.112	269	162	5	1	268	205	448	1.75e-32	134
MsaG027390	AT1G09060.1	34.242	330	143	8	318	582	570	890	9.21e-49	182
MsaG027390	AT1G09060.1	30.112	269	162	5	1	268	205	448	1.75e-32	134
MsaG027390	AT1G09060.4	34.242	330	143	8	318	582	584	904	1.00e-48	182
MsaG027390	AT1G09060.4	30.112	269	162	5	1	268	219	462	2.07e-32	133
MsaG027390	AT1G09060.3	34.242	330	143	8	318	582	584	904	1.00e-48	182
MsaG027390	AT1G09060.3	30.112	269	162	5	1	268	219	462	2.07e-32	133
MsaG027390	AT3G07610.2	43.147	197	110	2	1	197	154	348	5.32e-47	176
MsaG027390	AT3G07610.2	55.882	102	16	2	311	385	591	690	1.90e-23	105
MsaG027390	AT4G21430.1	33.106	293	156	4	328	581	620	911	6.73e-43	165
MsaG027390	AT4G21430.1	31.210	157	106	2	2	157	227	382	4.25e-13	73.2
MsaG027390	AT4G00990.3	61.062	113	39	2	419	531	671	778	8.33e-36	143
MsaG027390	AT4G00990.3	32.698	315	185	10	2	312	80	371	1.63e-34	139
MsaG027390	AT4G00990.3	42.763	152	57	3	311	433	458	608	4.27e-27	117
MsaG027390	AT4G00990.2	64.762	105	32	2	419	523	671	770	5.50e-35	141
MsaG027390	AT4G00990.2	32.698	315	185	10	2	312	80	371	1.24e-34	140
MsaG027390	AT4G00990.2	41.875	160	63	3	311	441	458	616	3.78e-27	117

Find 135 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTCACCATGCAGATAATT+TGG	0.149377	5:+43891712	None:intergenic
AGTACATGCGCTCATCATTA+TGG	0.215750	5:+43891966	None:intergenic
CTTCGTTGTGGGCAGCTTCT+AGG	0.236807	5:-43891775	MsaT027390.1:CDS
ATTCCTTAGATGGTGCTCTT+TGG	0.245043	5:-43890342	MsaT027390.1:CDS
TTCCTTAGATGGTGCTCTTT+GGG	0.262549	5:-43890341	MsaT027390.1:CDS
AGCATCAGGTGCTTTGTATC+TGG	0.288535	5:+43891675	None:intergenic
ATCCCAAGGATAATCATAAT+TGG	0.294597	5:-43891278	MsaT027390.1:intron
TCACATTTAATCTCAGGTTT+TGG	0.298428	5:+43891580	None:intergenic
TACCTCCAATTTGTCCTCTA+TGG	0.305460	5:+43887365	None:intergenic
TCATATTGCTAAAGCAAAAC+TGG	0.318714	5:-43890491	MsaT027390.1:CDS
TCTATGGACCTGTGATTTAC+TGG	0.329135	5:+43887381	None:intergenic
GCTCTTGTTTGAATCCTGTT+AGG	0.338447	5:-43891432	MsaT027390.1:CDS
GGAGCTACATGGTGATATTC+AGG	0.343116	5:-43890407	MsaT027390.1:CDS
ATGCCCGATGGTTAGATAAA+AGG	0.344516	5:+43888863	None:intergenic
AGAAGCATTTCAGAGAATTC+AGG	0.344845	5:-43890273	MsaT027390.1:CDS
ATTCCTACATGGTCAAAATC+TGG	0.346904	5:-43891637	MsaT027390.1:CDS
ATCCCAAAGAGCACCATCTA+AGG	0.353655	5:+43890339	None:intergenic
CCTTCAGATTTCGGACTTGA+TGG	0.363042	5:+43888651	None:intergenic
GTTGATGGGGATTCCTTAGA+TGG	0.367964	5:-43890352	MsaT027390.1:CDS
CTTGGCTTCTATCTCCTTCT+CGG	0.376051	5:+43892308	None:intergenic
GTACATGCGCTCATCATTAT+GGG	0.392626	5:+43891967	None:intergenic
ATATCATCTCTATTCCTAAC+AGG	0.396727	5:+43891418	None:intergenic
AAGTATTGAAATTGAAAGAT+TGG	0.403818	5:-43891251	MsaT027390.1:CDS
ACTAACTCAGAGATGCATTC+TGG	0.406256	5:+43891523	None:intergenic
AGAGGACGTACCTGCATTGC+AGG	0.406514	5:-43890305	MsaT027390.1:CDS
CAGCTCTTTGCAAAGAATCC+AGG	0.410316	5:-43891375	MsaT027390.1:CDS
TCTTCCACATTTAATACGAT+TGG	0.410475	5:-43892346	MsaT027390.1:CDS
CTTCACCATGCAGATAATTT+GGG	0.410728	5:+43891713	None:intergenic
GCAACTCTTTGTACTGTCCT+AGG	0.413078	5:-43891351	MsaT027390.1:CDS
GAAAATAGTTTGTTGGTTGA+TGG	0.415576	5:-43890367	MsaT027390.1:CDS
CCGTGATCACATTTAATCTC+AGG	0.429409	5:+43891574	None:intergenic
TCAAGCTGGGCACCAGCATC+AGG	0.440923	5:+43891661	None:intergenic
TTCAGATATTCCTGCAATGC+AGG	0.441628	5:+43890295	None:intergenic
AAGAGCTTTGAGCATATACT+TGG	0.441941	5:+43892368	None:intergenic
CTTCAGATTTCGGACTTGAT+GGG	0.446013	5:+43888652	None:intergenic
TATGGATTTGCTGAGGAGCT+TGG	0.447060	5:-43891141	MsaT027390.1:CDS
AAGATAAACTCAAAATCAAA+TGG	0.449448	5:+43891742	None:intergenic
GGAGCTTGGACGGGGTGATT+CGG	0.453767	5:-43891127	MsaT027390.1:CDS
GACAAAATCAAGTGCAACCT+TGG	0.456526	5:+43887458	None:intergenic
ATTTGCTGAGGAGCTTGGAC+GGG	0.458809	5:-43891136	MsaT027390.1:CDS
TCTGGCTTTAGAACTTCATC+AGG	0.461474	5:+43891189	None:intergenic
CAGGTCCATAGAGGACAAAT+TGG	0.466017	5:-43887370	MsaT027390.1:CDS
GCAAAGCTTGTTTGAAGTCA+CGG	0.474647	5:-43892548	MsaT027390.1:CDS
CATGCAGATAATTTGGGCCT+CGG	0.479508	5:+43891719	None:intergenic
GTCAAAGAAATGACAAGGGC+AGG	0.480713	5:-43892772	MsaT027390.1:CDS
ACGATTGGATCAAGAACAAA+TGG	0.480998	5:-43892331	MsaT027390.1:CDS
ACTACAGTCAATTAAAGAAA+AGG	0.482579	5:-43892427	MsaT027390.1:intron
AAAATAGTTTGTTGGTTGAT+GGG	0.484733	5:-43890366	MsaT027390.1:CDS
TGCGGAATTCCTCTGTCAAA+CGG	0.487229	5:+43887409	None:intergenic
GTTGCCGTGAGCTTCGTTGT+GGG	0.489346	5:-43891786	MsaT027390.1:CDS
TGTGATTTACTGGAAGTTTG+CGG	0.491065	5:+43887391	None:intergenic
CACTGTTGAAAATAGTTTGT+TGG	0.496892	5:-43890374	MsaT027390.1:CDS
ATGGTCAAAATCTGGGTGGC+AGG	0.497130	5:-43891629	MsaT027390.1:CDS
ACCAACTTTACCTCCTCTAC+AGG	0.497149	5:+43890235	None:intergenic
GTTGTGGGCAGCTTCTAGGT+GGG	0.507656	5:-43891771	MsaT027390.1:CDS
GATTTGCTGAGGAGCTTGGA+CGG	0.507712	5:-43891137	MsaT027390.1:CDS
GGAAACCAACGATCATCATC+AGG	0.509624	5:-43892400	MsaT027390.1:CDS
TGTATGTAGGTATTGAGCCT+TGG	0.510562	5:-43888724	MsaT027390.1:intron
CGTTGTGGGCAGCTTCTAGG+TGG	0.513332	5:-43891772	MsaT027390.1:CDS
AACAGGATTCAAACAAGAGC+AGG	0.514173	5:+43891435	None:intergenic
GCTCCCTCTTGTCTTGCTCA+AGG	0.519343	5:+43890425	None:intergenic
CAACTCTTTGTACTGTCCTA+GGG	0.522501	5:-43891350	MsaT027390.1:CDS
TGTTGCCGTGAGCTTCGTTG+TGG	0.522924	5:-43891787	MsaT027390.1:CDS
AAAGATTGGCCTCCTTTCAA+AGG	0.523135	5:-43891237	MsaT027390.1:CDS
TCTTTCAATTTCAATACTTG+AGG	0.529125	5:+43891255	None:intergenic
TTCCTACATGGTCAAAATCT+GGG	0.531797	5:-43891636	MsaT027390.1:CDS
ACCATGTAGGAATCTCAAGC+TGG	0.533279	5:+43891647	None:intergenic
TTTGTCTCTCCTGAAAATGT+TGG	0.534278	5:-43887441	MsaT027390.1:CDS
TCAAGAACAAATGGCCGAGA+AGG	0.538911	5:-43892322	MsaT027390.1:CDS
TGATCCAATCGTATTAAATG+TGG	0.539950	5:+43892342	None:intergenic
GAGATAGAAGCCAAGTTACA+AGG	0.542112	5:-43892300	MsaT027390.1:intron
TTCAGATTTCGGACTTGATG+GGG	0.544450	5:+43888653	None:intergenic
GTCAATTAAAGAAAAGGAGG+AGG	0.545970	5:-43892421	MsaT027390.1:CDS
TTTGCTGAGGAGCTTGGACG+GGG	0.546841	5:-43891135	MsaT027390.1:CDS
ATAATGATGAGCGCATGTAC+TGG	0.548032	5:-43891964	MsaT027390.1:intron
AGCTCTTTGCAAAGAATCCA+GGG	0.549815	5:-43891374	MsaT027390.1:CDS
TGTCCATTGCTCTCCTGTAG+AGG	0.552261	5:-43890248	MsaT027390.1:CDS
TTAGATAAAAGGTCTGATCA+TGG	0.552811	5:+43888874	None:intergenic
AGGTTGAGAGCACCTTTGAA+AGG	0.553650	5:+43891225	None:intergenic
TCAGATGCACCAAATGCAAA+CGG	0.554343	5:-43892745	MsaT027390.1:CDS
TAAAAGGTCTGATCATGGAT+GGG	0.555220	5:+43888879	None:intergenic
ATAAAAGGTCTGATCATGGA+TGG	0.557609	5:+43888878	None:intergenic
CTCAACCTGATGATGATCGT+TGG	0.558986	5:+43892395	None:intergenic
AAGGTATCCTCGATTGAAAG+AGG	0.564928	5:-43892616	MsaT027390.1:CDS
AGCATTCTGTGCAGCTTCTG+TGG	0.567239	5:+43891830	None:intergenic
GCACCTTGAGCAAGACAAGA+GGG	0.570458	5:-43890428	MsaT027390.1:CDS
AGATACAAAGCACCTGATGC+TGG	0.574516	5:-43891673	MsaT027390.1:CDS
AGCTTCTGAACAAAAGTCCA+AGG	0.575249	5:+43888707	None:intergenic
CCTGAGATTAAATGTGATCA+CGG	0.577057	5:-43891574	MsaT027390.1:CDS
CATCAGGCAGCTTCACAGCA+AGG	0.577705	5:+43891205	None:intergenic
CGAGGCCCAAATTATCTGCA+TGG	0.583825	5:-43891718	MsaT027390.1:CDS
AATTGTTCCTCTTTCAATCG+AGG	0.584177	5:+43892609	None:intergenic
GACGGGGTGATTCGGTGACT+AGG	0.584547	5:-43891119	MsaT027390.1:CDS
TGAGTTAGTATGTAAAGCAA+AGG	0.585122	5:-43891509	MsaT027390.1:CDS
AAATAGTTTGTTGGTTGATG+GGG	0.585934	5:-43890365	MsaT027390.1:CDS
GTCCATAGAGGACAAATTGG+AGG	0.587047	5:-43887367	MsaT027390.1:intron
AGTAAATCACAGGTCCATAG+AGG	0.591019	5:-43887379	MsaT027390.1:CDS
CAAGACAAGAGGGAGCTACA+TGG	0.591607	5:-43890418	MsaT027390.1:CDS
GCTGCCCACAACGAAGCTCA+CGG	0.592775	5:+43891782	None:intergenic
TAATATCTATCTACAGCCCT+AGG	0.594797	5:+43891334	None:intergenic
TTGCAGTTACCACAGCAGAC+AGG	0.598583	5:+43892573	None:intergenic
GCAAACTTCCAGTAAATCAC+AGG	0.600882	5:-43887389	MsaT027390.1:CDS
TTGTGGGCAGCTTCTAGGTG+GGG	0.602777	5:-43891770	MsaT027390.1:CDS
AAAAGGTCTGATCATGGATG+GGG	0.603357	5:+43888880	None:intergenic
ACAGTCAATTAAAGAAAAGG+AGG	0.606305	5:-43892424	MsaT027390.1:intron
ACAGTACAAAGAGTTGCCCC+TGG	0.608816	5:+43891357	None:intergenic
CCCAGCTTGAGATTCCTACA+TGG	0.609328	5:-43891648	MsaT027390.1:CDS
TTGAGAGCACCTTTGAAAGG+AGG	0.613142	5:+43891228	None:intergenic
AACGAAGCTCACGGCAACAG+AGG	0.614200	5:+43891791	None:intergenic
GAGGCATGTCCTGTCTGCTG+TGG	0.617423	5:-43892582	MsaT027390.1:CDS
AGCACCTTGAGCAAGACAAG+AGG	0.618569	5:-43890429	MsaT027390.1:CDS
TATTGCTTATGGATTTGCTG+AGG	0.619448	5:-43891148	MsaT027390.1:CDS
GAAAGAGGAACAATTTGCTG+AGG	0.620045	5:-43892601	MsaT027390.1:CDS
TGAAGTTCTAAAGCCAGACA+TGG	0.620406	5:-43891184	MsaT027390.1:CDS
TCAAAGAAATGACAAGGGCA+GGG	0.621822	5:-43892771	MsaT027390.1:CDS
CCATGTAGGAATCTCAAGCT+GGG	0.624894	5:+43891648	None:intergenic
GGGTGGCAGGCTGATAGTGA+CGG	0.631761	5:-43891616	MsaT027390.1:CDS
CTACATGGTGATATTCAGGA+TGG	0.633510	5:-43890403	MsaT027390.1:CDS
AATGAAACTTCAAAATGCAG+AGG	0.634474	5:-43891473	MsaT027390.1:CDS
CCATTGCTCTCCTGTAGAGG+AGG	0.634485	5:-43890245	MsaT027390.1:intron
CCATCAAGTCCGAAATCTGA+AGG	0.635792	5:-43888651	MsaT027390.1:intron
TCAATGTCAAAGAAATGACA+AGG	0.646775	5:-43892777	MsaT027390.1:CDS
TCAGATTTCGGACTTGATGG+GGG	0.647731	5:+43888654	None:intergenic
CACGGCAACAGAGGAGACAA+AGG	0.649807	5:+43891800	None:intergenic
CCTCCTCTACAGGAGAGCAA+TGG	0.652097	5:+43890245	None:intergenic
TGAGTGCTTCCGTTTGACAG+AGG	0.665002	5:-43887418	MsaT027390.1:CDS
GAAGTTCTAAAGCCAGACAT+GGG	0.665837	5:-43891183	MsaT027390.1:CDS
TACATGCGCTCATCATTATG+GGG	0.667563	5:+43891968	None:intergenic
ATGATATACACAATAACACA+AGG	0.668038	5:-43891402	MsaT027390.1:CDS
CGTATATGCTATGATTGATG+TGG	0.680480	5:-43886910	MsaT027390.1:CDS
CAATGTCAAAGAAATGACAA+GGG	0.710751	5:-43892776	MsaT027390.1:CDS
CTACATGGTCAAAATCTGGG+TGG	0.720269	5:-43891633	MsaT027390.1:CDS
GCTCTTTGCAAAGAATCCAG+GGG	0.726479	5:-43891373	MsaT027390.1:CDS
CTGCGTCAGACATATCACAA+TGG	0.731463	5:+43891094	None:intergenic
TGATATACACAATAACACAA+GGG	0.738813	5:-43891401	MsaT027390.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	43886878	43892807	43886878	ID=MsaG027390
Chr5	mRNA	43886878	43892807	43886878	ID=MsaT027390.1;Parent=MsaG027390
Chr5	exon	43886878	43886946	43886878	ID=MsaT027390.1.exon11;Parent=MsaT027390.1
Chr5	CDS	43886878	43886946	43886878	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43887368	43887487	43887368	ID=MsaT027390.1.exon10;Parent=MsaT027390.1
Chr5	CDS	43887368	43887487	43887368	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43888652	43888737	43888652	ID=MsaT027390.1.exon9;Parent=MsaT027390.1
Chr5	CDS	43888652	43888737	43888652	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43888838	43888910	43888838	ID=MsaT027390.1.exon8;Parent=MsaT027390.1
Chr5	CDS	43888838	43888910	43888838	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43890246	43890527	43890246	ID=MsaT027390.1.exon7;Parent=MsaT027390.1
Chr5	CDS	43890246	43890527	43890246	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43891095	43891292	43891095	ID=MsaT027390.1.exon6;Parent=MsaT027390.1
Chr5	CDS	43891095	43891292	43891095	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43891348	43891882	43891348	ID=MsaT027390.1.exon5;Parent=MsaT027390.1
Chr5	CDS	43891348	43891882	43891348	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43891965	43892028	43891965	ID=MsaT027390.1.exon4;Parent=MsaT027390.1
Chr5	CDS	43891965	43892028	43891965	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43892301	43892442	43892301	ID=MsaT027390.1.exon3;Parent=MsaT027390.1
Chr5	CDS	43892301	43892442	43892301	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43892536	43892635	43892536	ID=MsaT027390.1.exon2;Parent=MsaT027390.1
Chr5	CDS	43892536	43892635	43892536	ID=cds.MsaT027390.1;Parent=MsaT027390.1
Chr5	exon	43892713	43892807	43892713	ID=MsaT027390.1.exon1;Parent=MsaT027390.1
Chr5	CDS	43892713	43892807	43892713	ID=cds.MsaT027390.1;Parent=MsaT027390.1

Gene Sequence

>MsaT027390.1

ATGTGTCATCAATGTCAAAGAAATGACAAGGGCAGGGTTGTCAGATGCACCAAATGCAAACGGAAAAGATATTGTGTAGACTGTATAAAAAATTGGTATCCTCGATTGAAAGAGGAACAATTTGCTGAGGCATGTCCTGTCTGCTGTGGTAACTGCAATTGCAAAGCTTGTTTGAAGTCACGGAAACTTATCGATTCAATTAAAGAAAAGGAGGAGGAAACCAACGATCATCATCAGGTTGAGCGCTCCAAGTATATGCTCAAAGCTCTTCTTCCACATTTAATACGATTGGATCAAGAACAAATGGCCGAGAAGGAGATAGAAGCCAAGTTACAAGGTCTCTCACTCTCTGAGCTAAAAATTAAAAAATCAAATCCCCATAATGATGAGCGCATGTACTGTGACAACTGCAAAACATCAATATTTGACTACCACAGAAGCTGCACAGAATGCTCTTTTGACCTTTGTCTCCTCTGTTGCCGTGAGCTTCGTTGTGGGCAGCTTCTAGGTGGGGCAGATCCATTTGATTTTGAGTTTATCTTCCGAGGCCCAAATTATCTGCATGGTGAAGTTGCGAAGAAAGTAACCAGATACAAAGCACCTGATGCTGGTGCCCAGCTTGAGATTCCTACATGGTCAAAATCTGGGTGGCAGGCTGATAGTGACGGCAATATTCCCTGTCCAAAACCTGAGATTAAATGTGATCACGGTTACCTTGAACTGAAAAGCGTATTTTCGCCAGAATGCATCTCTGAGTTAGTATGTAAAGCAAAGGAACTTGCAGATTCAATGAAACTTCAAAATGCAGAGGTGACTCTTGATAATACCTGCTCTTGTTTGAATCCTGTTAGGAATAGAGATGATATACACAATAACACAAGGGAAGCAGCTCTTTGCAAAGAATCCAGGGGCAACTCTTTGTACTGTCCTAGGGCTGATAATCATAATTGGCCTCAAGTATTGAAATTGAAAGATTGGCCTCCTTTCAAAGGTGCTCTCAACCTTGCTGTGAAGCTGCCTGATGAAGTTCTAAAGCCAGACATGGGACCAAAAACATATATTGCTTATGGATTTGCTGAGGAGCTTGGACGGGGTGATTCGGTGACTAGGCTCCATTGTGATATGTCTGACGCAGTAAATGTGTTGACTCATATTGCTAAAGCAAAACTGGATCGTGTTATTATTGACGCAATTAAGAAATTGAAACAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATATTCAGGATGGAGAGACCACTGTTGAAAATAGTTTGTTGGTTGATGGGGATTCCTTAGATGGTGCTCTTTGGGATATTTTTCGGAGAGAGGACGTACCTGCATTGCAGGAATATCTGAAGAAGCATTTCAGAGAATTCAGGCATGTCCATTGCTCTCCTGTAGAGGAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCATCGGGCATAAGAAAAAGCTGAAGGAAGAGTATGGTATTGAGCCTTGGACTTTTGTTCAGAAGCTTGGAGATGCAGTTTTCATTCCAGCTGGTTGCCCCCATCAAGTCCGAAATCTGAAGTCATGCACCAAGGTTGCACTTGATTTTGTCTCTCCTGAAAATGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTGAAGAAAATGATCGTATATGCTATGATTGATGTGGTCAAAAAGTTGGAGAATGCAAGGAGCCAATGA

Protein sequence

>MsaT027390.1

MCHQCQRNDKGRVVRCTKCKRKRYCVDCIKNWYPRLKEEQFAEACPVCCGNCNCKACLKSRKLIDSIKEKEEETNDHHQVERSKYMLKALLPHLIRLDQEQMAEKEIEAKLQGLSLSELKIKKSNPHNDERMYCDNCKTSIFDYHRSCTECSFDLCLLCCRELRCGQLLGGADPFDFEFIFRGPNYLHGEVAKKVTRYKAPDAGAQLEIPTWSKSGWQADSDGNIPCPKPEIKCDHGYLELKSVFSPECISELVCKAKELADSMKLQNAEVTLDNTCSCLNPVRNRDDIHNNTREAALCKESRGNSLYCPRADNHNWPQVLKLKDWPPFKGALNLAVKLPDEVLKPDMGPKTYIAYGFAEELGRGDSVTRLHCDMSDAVNVLTHIAKAKLDRVIIDAIKKLKQKHLEQDKRELHGDIQDGETTVENSLLVDGDSLDGALWDIFRREDVPALQEYLKKHFREFRHVHCSPVEEVIHPIHDQTFYLTIGHKKKLKEEYGIEPWTFVQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSIEDKLEVKKMIVYAMIDVVKKLENARSQ*