AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027429


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027429 MtrunA17_Chr4g0033281 83.901 323 34 3 1 305 1 323 0.0 517
MsaG027429 MtrunA17_Chr4g0033291 79.290 338 31 6 1 308 1 329 1.52e-179 498
MsaG027429 MtrunA17_Chr4g0033391 76.807 332 36 5 1 305 1 318 1.25e-175 488
MsaG027429 MtrunA17_Chr2g0307131 58.266 369 78 7 1 307 1 355 1.60e-127 367
MsaG027429 MtrunA17_Chr6g0468491 61.465 314 93 7 23 308 1 314 4.89e-121 350
MsaG027429 MtrunA17_Chr2g0307141 56.604 371 87 8 1 308 1 360 1.60e-117 342
MsaG027429 MtrunA17_Chr6g0468461 51.064 329 110 7 1 291 1 316 2.02e-91 276
MsaG027429 MtrunA17_Chr7g0225901 56.618 272 75 7 23 283 1 240 1.01e-88 265
MsaG027429 MtrunA17_Chr4g0054621 48.266 346 137 11 1 308 1 342 2.46e-86 262
MsaG027429 MtrunA17_Chr4g0054581 57.741 239 82 4 88 308 142 379 7.48e-82 252
MsaG027429 MtrunA17_Chr4g0054581 55.696 79 28 3 1 77 1 74 9.09e-15 74.3
MsaG027429 MtrunA17_Chr5g0400621 42.284 324 144 9 17 307 20 333 3.42e-62 200
MsaG027429 MtrunA17_Chr5g0400661 35.088 342 157 9 13 303 7 334 3.70e-45 156
MsaG027429 MtrunA17_Chr4g0002801 41.667 204 98 6 122 304 128 331 6.51e-42 147
MsaG027429 MtrunA17_Chr5g0400641 52.500 160 58 5 144 289 1 156 9.57e-42 142
MsaG027429 MtrunA17_Chr2g0280161 43.243 185 92 2 134 305 168 352 1.55e-40 144
MsaG027429 MtrunA17_Chr4g0054551 39.130 207 110 4 107 302 123 324 7.15e-40 142
MsaG027429 MtrunA17_Chr8g0336681 39.175 194 101 2 128 304 143 336 3.39e-39 140
MsaG027429 MtrunA17_Chr4g0054561 38.647 207 111 4 107 302 123 324 7.01e-39 139
MsaG027429 MtrunA17_Chr4g0049331 39.614 207 103 3 115 305 109 309 1.09e-38 138
MsaG027429 MtrunA17_Chr4g0002781 37.872 235 120 7 92 304 120 350 1.40e-38 139
MsaG027429 MtrunA17_Chr2g0333621 39.409 203 111 3 117 307 113 315 3.75e-38 137
MsaG027429 MtrunA17_Chr4g0054571 45.405 185 84 4 138 305 147 331 1.49e-37 136
MsaG027429 MtrunA17_Chr2g0333641 38.182 220 118 5 93 302 95 306 1.26e-36 133
MsaG027429 MtrunA17_Chr5g0400671 35.749 207 105 4 116 303 126 323 3.71e-36 132
MsaG027429 MtrunA17_Chr2g0333541 43.316 187 90 5 130 301 81 266 7.76e-36 130
MsaG027429 MtrunA17_Chr2g0333611 38.503 187 103 5 130 305 32 217 1.74e-33 122
MsaG027429 MtrunA17_Chr7g0249811 37.864 206 124 3 100 304 127 329 2.79e-33 125
MsaG027429 MtrunA17_Chr4g0002831 37.423 163 95 5 143 302 45 203 6.99e-26 102
MsaG027429 MtrunA17_Chr7g0233441 37.931 145 78 2 164 302 57 195 7.84e-24 96.3
MsaG027429 MtrunA17_Chr4g0002851 39.568 139 76 2 164 302 65 195 8.54e-23 93.6
MsaG027429 MtrunA17_Chr8g0366751 30.120 166 85 2 133 267 444 609 7.38e-16 78.2
MsaG027429 MtrunA17_Chr8g0346161 26.891 238 140 8 56 260 187 423 1.73e-15 76.6
MsaG027429 MtrunA17_Chr1g0185621 31.944 144 76 3 141 264 475 616 1.40e-14 74.3
MsaG027429 MtrunA17_Chr7g0275821 30.519 154 101 2 137 284 244 397 1.92e-14 73.6
MsaG027429 MtrunA17_Chr8g0346151 30.000 150 76 5 115 240 248 392 3.54e-14 72.8
MsaG027429 MtrunA17_Chr5g0411341 29.282 181 75 4 126 267 474 640 1.59e-13 70.9
MsaG027429 MtrunA17_Chr7g0260841 36.082 97 52 1 108 194 312 408 1.97e-12 67.4
MsaG027429 MtrunA17_Chr8g0365711 27.684 177 101 6 126 280 227 398 6.60e-12 65.9
MsaG027429 MtrunA17_Chr3g0131691 26.596 188 117 3 127 294 284 470 8.42e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027429 AT4G37850.2 41.379 203 98 6 121 305 128 327 3.33e-37 135
MsaG027429 AT4G37850.1 41.379 203 98 6 121 305 128 327 3.33e-37 135
MsaG027429 AT2G22750.3 33.429 347 166 13 8 306 4 333 1.13e-36 134
MsaG027429 AT2G22750.5 32.941 340 164 12 15 306 6 329 3.26e-36 132
MsaG027429 AT2G22760.1 40.212 189 97 5 130 303 108 295 5.53e-36 131
MsaG027429 AT2G22750.4 33.429 347 165 14 8 306 4 332 2.56e-35 130
MsaG027429 AT2G22750.2 37.759 241 112 7 99 306 70 305 1.67e-34 127
MsaG027429 AT2G22750.1 37.759 241 111 8 99 306 70 304 2.45e-33 124
MsaG027429 AT2G22770.1 37.766 188 92 2 140 302 131 318 7.62e-31 118
MsaG027429 AT2G22760.2 40.476 168 85 4 130 283 108 274 5.33e-30 115
MsaG027429 AT5G46760.1 32.178 202 98 8 123 287 393 592 1.27e-18 86.7
MsaG027429 AT4G17880.1 32.110 218 118 7 78 265 340 557 1.01e-17 84.0
MsaG027429 AT1G32640.1 32.500 160 87 3 126 267 433 589 2.36e-15 77.0
MsaG027429 AT2G46510.1 37.931 116 54 3 133 238 377 484 6.53e-15 75.5
MsaG027429 AT1G01260.1 29.114 158 87 3 131 280 420 560 1.53e-13 71.2
MsaG027429 AT1G01260.2 29.114 158 87 3 131 280 420 560 1.53e-13 71.2
MsaG027429 AT1G01260.3 29.114 158 87 3 131 280 420 560 1.53e-13 71.2
MsaG027429 AT4G09820.1 25.000 292 170 9 20 306 269 516 3.09e-13 70.5
MsaG027429 AT4G16430.1 46.575 73 35 1 126 194 301 373 8.32e-13 68.9
MsaG027429 AT5G46830.1 54.839 62 24 1 137 194 335 396 4.02e-12 67.0
MsaG027429 AT5G41315.2 27.869 183 94 6 132 279 440 619 6.21e-11 63.5
MsaG027429 AT5G41315.3 27.869 183 94 6 132 279 437 616 6.31e-11 63.5
MsaG027429 AT5G41315.1 27.869 183 94 6 132 279 432 611 7.02e-11 63.5

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGTTCCTAACCTTAAAA+AGG 0.267726 5:+44676458 MsaT027429.1:CDS
CAGCAGCTGGTTATCTGATT+TGG 0.268145 5:+44675864 MsaT027429.1:CDS
GGGGCTTCTGGTTAAAATTA+TGG 0.308732 5:+44677313 MsaT027429.1:CDS
CAAGGAGCACAAGGGGCTTC+TGG 0.325264 5:+44677301 MsaT027429.1:CDS
GCTAAGATCATTTGTGGAAA+TGG 0.327969 5:-44676160 None:intergenic
TCAAGAACAAAACAAACAAT+TGG 0.334442 5:+44676134 MsaT027429.1:CDS
CAAGTGGAAGCAAGAGTGTT+AGG 0.334987 5:+44677251 MsaT027429.1:CDS
ATTCATGAAACAATTGATAA+TGG 0.338552 5:+44676192 MsaT027429.1:CDS
ATCAGTGTCTCCAACCGAAT+TGG 0.364748 5:+44676269 MsaT027429.1:CDS
CTGCAAGAGCAATGAAGCTC+TGG 0.379324 5:-44676434 None:intergenic
CAAGAACAAAACAAACAATT+GGG 0.390107 5:+44676135 MsaT027429.1:CDS
ATCGCTGCATATGCATGGTT+TGG 0.407010 5:-44677174 None:intergenic
ACTCTTGCTTCCACTTGAAA+TGG 0.409697 5:-44677245 None:intergenic
AGAAACATCAAAAGGGTCAT+TGG 0.411401 5:+44676311 MsaT027429.1:CDS
AATAACAGTGTCTTACCCTT+TGG 0.416567 5:+44677368 MsaT027429.1:CDS
GCCCCTTGTGCTCCTTGCAT+TGG 0.417499 5:-44677295 None:intergenic
TCACAAGTTCCTTTATGCTC+AGG 0.418425 5:-44677618 None:intergenic
CATCTGCATCAAGAAAATTC+AGG 0.444660 5:-44676074 None:intergenic
TAGGAAAACAAGTGTTGATT+CGG 0.449123 5:+44677270 MsaT027429.1:CDS
AGCAAAAGGCCTCGTGCTCA+TGG 0.450095 5:+44676366 MsaT027429.1:CDS
CACTCAACAGAAACATCAAA+AGG 0.454180 5:+44676303 MsaT027429.1:CDS
TGACTTGCTAAGATCATTTG+TGG 0.465292 5:-44676166 None:intergenic
TTGATGATACTTTCATTGCT+CGG 0.501130 5:-44675840 None:intergenic
GGTAGCTACCTGAGCAATGA+TGG 0.511294 5:-44677411 None:intergenic
TTCTTGATGCAGATGTGGGA+GGG 0.544131 5:+44676082 MsaT027429.1:CDS
CGACATTACCATCATTGCTC+AGG 0.544333 5:+44677403 MsaT027429.1:CDS
GTTGATTCGGTTCCAATGCA+AGG 0.545125 5:+44677283 MsaT027429.1:CDS
GTTCCAATGCAAGGAGCACA+AGG 0.563766 5:+44677292 MsaT027429.1:CDS
TGCAAGAGCAATGAAGCTCT+GGG 0.565077 5:-44676433 None:intergenic
ACTCAACAGAAACATCAAAA+GGG 0.567021 5:+44676304 MsaT027429.1:CDS
TCTTCATCGCTGCATATGCA+TGG 0.570607 5:-44677179 None:intergenic
AAGTATCATCAATCAGCAGC+TGG 0.573007 5:+44675851 MsaT027429.1:CDS
AAGGCCTCGTGCTCATGGAA+AGG 0.577326 5:+44676371 MsaT027429.1:CDS
TGTTGCTTCACCATTTCAAG+TGG 0.586046 5:+44677235 MsaT027429.1:CDS
TTTCTTGATGCAGATGTGGG+AGG 0.588172 5:+44676081 MsaT027429.1:CDS
GTCGAGTGTAGAATCTCCAA+AGG 0.590011 5:-44677384 None:intergenic
TCCAATGCAAGGAGCACAAG+GGG 0.595381 5:+44677294 MsaT027429.1:CDS
TCATGGAAAGGATCACATCA+TGG 0.611063 5:+44676383 MsaT027429.1:CDS
TCGAGTGTAGAATCTCCAAA+GGG 0.615421 5:-44677383 None:intergenic
CTGCATATGCATGGTTTGGT+CGG 0.617639 5:-44677170 None:intergenic
AGGGTACAACCTGAGCATAA+AGG 0.620360 5:+44677609 MsaT027429.1:CDS
CGAATACAATATATTTGCTG+AGG 0.624364 5:+44676044 MsaT027429.1:CDS
TGAAGATTCGTCATCATGTG+AGG 0.642706 5:+44677196 MsaT027429.1:CDS
TTCCAATGCAAGGAGCACAA+GGG 0.644827 5:+44677293 MsaT027429.1:CDS
AAGAACAAAACAAACAATTG+GGG 0.650416 5:+44676136 MsaT027429.1:CDS
ACTTGTGAAGAACGTACGCA+TGG 0.661373 5:+44677633 MsaT027429.1:CDS
TGATCCTTTCCATGAGCACG+AGG 0.668624 5:-44676375 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 44675823 44677681 44675823 ID=MsaG027429
Chr5 mRNA 44675823 44677681 44675823 ID=MsaT027429.1;Parent=MsaG027429
Chr5 exon 44675823 44675885 44675823 ID=MsaT027429.1.exon1;Parent=MsaT027429.1
Chr5 CDS 44675823 44675885 44675823 ID=cds.MsaT027429.1;Parent=MsaT027429.1
Chr5 exon 44676036 44676479 44676036 ID=MsaT027429.1.exon2;Parent=MsaT027429.1
Chr5 CDS 44676036 44676479 44676036 ID=cds.MsaT027429.1;Parent=MsaT027429.1
Chr5 exon 44677086 44677424 44677086 ID=MsaT027429.1.exon3;Parent=MsaT027429.1
Chr5 CDS 44677086 44677424 44677086 ID=cds.MsaT027429.1;Parent=MsaT027429.1
Chr5 exon 44677601 44677681 44677601 ID=MsaT027429.1.exon4;Parent=MsaT027429.1
Chr5 CDS 44677601 44677681 44677601 ID=cds.MsaT027429.1;Parent=MsaT027429.1
Gene Sequence

>MsaT027429.1

ATGGAGGAAATCAACAACCGAGCAATGAAAGTATCATCAATCAGCAGCTGGTTATCTGATTTGGAAATGGACGAATACAATATATTTGCTGAGGAATGCAACCTGAATTTTCTTGATGCAGATGTGGGAGGGTTTCTTTCAGATGACATAGCTAATGTATTTCAAGAACAAAACAAACAATTGGGGTCCATTTCCACAAATGATCTTAGCAAGTCATTCATTCATGAAACAATTGATAATGGTGACAAAATCAATGAATCTCTTTCACCAAATATTTCAAAAGAGAATGAAACAATATCAGTGTCTCCAACCGAATTGGAAAACATGAATCACTCAACAGAAACATCAAAAGGGTCATTGGAAAATGAAAAGTTTGAAACAAAAACCTCAAAAAGCAAAAGGCCTCGTGCTCATGGAAAGGATCACATCATGGCTGAGAGAAATCGAAGAGAGAAACTCACCCAGAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTTAAAAAGATGGACAAGTTATCTGTACTAATTGACACTATCAAATACTTGAAAGAGCTTAAAAACCGTTTGGAAGTTGTGGAAAAAAAGTCACCGACCAAACCATGCATATGCAGCGATGAAGATTCGTCATCATGTGAGGATAACATTGAATGTGTTGTTGCTTCACCATTTCAAGTGGAAGCAAGAGTGTTAGGAAAACAAGTGTTGATTCGGTTCCAATGCAAGGAGCACAAGGGGCTTCTGGTTAAAATTATGGTCGAAATTCAAAAATTTCAACTATTTGTTGTCAATAACAGTGTCTTACCCTTTGGAGATTCTACACTCGACATTACCATCATTGCTCAGTTGGGTGAAGGGTACAACCTGAGCATAAAGGAACTTGTGAAGAACGTACGCATGGCATTGTTGAAGTTTACGTCATCATAA

Protein sequence

>MsaT027429.1

MEEINNRAMKVSSISSWLSDLEMDEYNIFAEECNLNFLDADVGGFLSDDIANVFQEQNKQLGSISTNDLSKSFIHETIDNGDKINESLSPNISKENETISVSPTELENMNHSTETSKGSLENEKFETKTSKSKRPRAHGKDHIMAERNRREKLTQSFIALAALVPNLKKMDKLSVLIDTIKYLKELKNRLEVVEKKSPTKPCICSDEDSSSCEDNIECVVASPFQVEARVLGKQVLIRFQCKEHKGLLVKIMVEIQKFQLFVVNNSVLPFGDSTLDITIIAQLGEGYNLSIKELVKNVRMALLKFTSS*