AlfalfaGEDB | MsaG027521

M. sativa ssp. caerulea / MsaG027521

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027521	AES97588.1	93.671	158	10	0	1	158	1	158	2.83e-95	282

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027521	tr\|G7K1J4\|G7K1J4_MEDTR	93.671	158	10	0	1	158	1	158	1.35e-95	282

TFs/TRs:

Gene ID	Type	Classification
MsaG027521	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000024	MsaG027521	0.818699	2.985460e-52	6.345082e-50
MsaG001238	MsaG027521	0.821299	7.637677e-53	1.738554e-50
MsaG003187	MsaG027521	0.837082	1.174783e-56	4.189295e-54
MsaG003200	MsaG027521	0.823370	2.538169e-53	6.107848e-51
MsaG003201	MsaG027521	0.840559	1.496150e-57	5.943912e-55
MsaG003202	MsaG027521	0.848805	9.196911e-60	4.785259e-57
MsaG003203	MsaG027521	0.823369	2.538949e-53	6.109658e-51
MsaG003239	MsaG027521	0.845415	7.732270e-59	3.591902e-56
MsaG003821	MsaG027521	0.822136	4.900193e-53	1.140562e-50
MsaG004226	MsaG027521	0.803772	5.007662e-49	7.378770e-47
MsaG004462	MsaG027521	0.823575	2.273797e-53	5.502137e-51
MsaG004504	MsaG027521	0.845203	8.820397e-59	4.068486e-56
MsaG004565	MsaG027521	0.812610	6.685908e-51	1.217278e-48
MsaG005329	MsaG027521	0.814371	2.753966e-51	5.240044e-49
MsaG005442	MsaG027521	0.842388	4.959796e-58	2.088402e-55
MsaG005443	MsaG027521	0.821916	5.510157e-53	1.274950e-50
MsaG005444	MsaG027521	0.828256	1.779102e-54	4.900136e-52
MsaG005506	MsaG027521	0.803165	6.681242e-49	9.707662e-47
MsaG005508	MsaG027521	0.810960	1.522822e-50	2.662153e-48
MsaG005864	MsaG027521	0.845205	8.808769e-59	4.063425e-56
MsaG006204	MsaG027521	0.821057	8.677883e-53	1.962779e-50
MsaG007353	MsaG027521	0.802909	7.540790e-49	1.089195e-46
MsaG007400	MsaG027521	0.840119	1.946545e-57	7.627269e-55
MsaG009138	MsaG027521	0.838642	4.689060e-57	1.754387e-54
MsaG010263	MsaG027521	0.816811	7.925926e-52	1.604405e-49
MsaG012139	MsaG027521	0.847947	1.584216e-59	8.008780e-57
MsaG012140	MsaG027521	0.808559	4.972174e-50	8.201075e-48
MsaG012196	MsaG027521	0.867644	2.370912e-65	2.492442e-62
MsaG012736	MsaG027521	0.813504	4.267156e-51	7.944401e-49
MsaG013325	MsaG027521	0.839842	2.296790e-57	8.922073e-55
MsaG015202	MsaG027521	0.837650	8.414508e-57	3.053140e-54
MsaG015311	MsaG027521	0.805835	1.865451e-49	2.883705e-47
MsaG017133	MsaG027521	0.908744	2.937202e-81	2.558626e-77
MsaG018134	MsaG027521	0.874610	1.220853e-67	1.726109e-64
MsaG018529	MsaG027521	0.817328	6.073744e-52	1.245993e-49
MsaG019361	MsaG027521	0.815536	1.522654e-51	2.983606e-49
MsaG019639	MsaG027521	0.856321	6.759991e-62	4.582171e-59
MsaG020347	MsaG027521	0.825058	1.022702e-53	2.576764e-51
MsaG023374	MsaG027521	0.808384	5.415260e-50	8.894245e-48
MsaG023808	MsaG027521	0.895746	1.611873e-75	6.466367e-72
MsaG023971	MsaG027521	0.802266	1.021961e-48	1.454527e-46
MsaG025469	MsaG027521	0.820957	9.148499e-53	2.063757e-50
MsaG026714	MsaG027521	0.813927	3.446928e-51	6.486001e-49
MsaG027039	MsaG027521	0.830629	4.746918e-55	1.398652e-52
MsaG027524	MsaG027521	0.912441	4.755733e-83	5.269943e-79
MsaG027521	MsaG027568	0.865403	1.211801e-64	1.163394e-61
MsaG027521	MsaG029569	0.817015	7.134927e-52	1.451966e-49
MsaG027521	MsaG030084	0.820513	1.155897e-52	2.576997e-50
MsaG027521	MsaG030313	0.830802	4.308598e-55	1.275861e-52
MsaG027521	MsaG030401	0.806267	1.514415e-49	2.365192e-47
MsaG027521	MsaG030402	0.816441	9.586867e-52	1.922206e-49
MsaG027521	MsaG031236	0.804629	3.326348e-49	4.999634e-47
MsaG027521	MsaG031735	0.814469	2.620489e-51	4.998236e-49
MsaG027521	MsaG033014	0.890221	2.637208e-73	7.860845e-70
MsaG027521	MsaG033698	0.808512	5.086315e-50	8.379930e-48
MsaG027521	MsaG033921	0.845114	9.315521e-59	4.284730e-56
MsaG027521	MsaG034148	0.818202	3.864191e-52	8.107783e-50
MsaG027521	MsaG034828	0.814108	3.144753e-51	5.944233e-49
MsaG027521	MsaG035121	0.823565	2.285508e-53	5.529056e-51
MsaG027521	MsaG041901	0.803171	6.660755e-49	9.679279e-47
MsaG027521	MsaG041903	0.823255	2.698414e-53	6.473368e-51
MsaG027521	MsaG041904	0.805624	2.064321e-49	3.175405e-47
MsaG027521	MsaG044447	0.805940	1.773515e-49	2.748359e-47
MsaG027521	MsaG044752	0.879714	2.097215e-69	3.735347e-66
MsaG027521	MsaG045445	0.817667	5.098656e-52	1.055071e-49
MsaG027521	MsaG045611	0.806808	1.165644e-49	1.843795e-47
MsaG027521	MsaG046118	0.878833	4.283800e-69	7.326859e-66
MsaG027521	MsaG046119	0.838078	6.542668e-57	2.405369e-54
MsaG027521	MsaG046807	0.809993	2.457321e-50	4.195699e-48
MsaG027521	MsaG046863	0.886423	7.501645e-72	1.843103e-68
MsaG027521	MsaG047054	0.824237	1.592754e-53	3.924034e-51
MsaG027521	MsaG002437	0.831828	2.415750e-55	7.370694e-53
MsaG027521	MsaG000067	0.841901	6.664632e-58	2.762914e-55
MsaG027521	MsaG002583	0.808973	4.058425e-50	6.761108e-48
MsaG027521	MsaG002081	0.806952	1.087333e-49	1.725740e-47
MsaG027521	MsaG026411	0.806643	1.262991e-49	1.990043e-47
MsaG027521	MsaG027699	0.813364	4.578474e-51	8.494200e-49
MsaG027521	MsaG030963	0.864963	1.663107e-64	1.568979e-61
MsaG027521	MsaG034358	0.809120	3.776588e-50	6.313876e-48
MsaG027521	MsaG036473	0.808851	4.309200e-50	7.157917e-48
MsaG027521	MsaG037204	0.837475	9.325659e-57	3.365870e-54
MsaG027521	MsaG043458	0.824464	1.409900e-53	3.495099e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027521	MtrunA17_Chr5g0423491	93.671	158	10	0	1	158	1	158	2.60e-99	282
MsaG027521	MtrunA17_Chr1g0166461	58.046	174	50	5	1	158	1	167	1.03e-58	180
MsaG027521	MtrunA17_Chr1g0170601	47.368	171	59	7	1	158	1	153	8.37e-33	114
MsaG027521	MtrunA17_Chr1g0187511	35.032	157	78	6	11	158	4	145	3.63e-14	65.9
MsaG027521	MtrunA17_Chr1g0187541	32.624	141	80	4	11	144	6	138	9.76e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAAATTGATTTCATTGATT+TGG	0.224195	5:+46731066	MsaT027521.1:CDS
CATTTGAAGATTCTCCTTCT+AGG	0.234818	5:-46731000	None:intergenic
GGCCGAATCACCGTTGGTTT+TGG	0.304502	5:-46730896	None:intergenic
AAATCCAATGGATTAACATC+TGG	0.363954	5:-46731109	None:intergenic
TGCTTCGATTGGTAAGCATT+TGG	0.404776	5:-46730977	None:intergenic
AACCAAAACCAACGGTGATT+CGG	0.418396	5:+46730894	MsaT027521.1:CDS
CTGGTGAGTTTAGTAATATT+AGG	0.423811	5:+46730675	MsaT027521.1:CDS
TGAATAAAGCTTTCTGATTG+TGG	0.433760	5:-46730801	None:intergenic
TACCAATCGAAGCACCTAGA+AGG	0.448280	5:+46730986	MsaT027521.1:CDS
CTCCTTCTAGGTGCTTCGAT+TGG	0.488901	5:-46730988	None:intergenic
GAAGGAGAATCTTCAAATGA+TGG	0.491518	5:+46731004	MsaT027521.1:CDS
TAACCCAGATGTTAATCCAT+TGG	0.496678	5:+46731105	MsaT027521.1:CDS
AATCCAATGGATTAACATCT+GGG	0.500492	5:-46731108	None:intergenic
ATCTTCAAATGATGGTACTA+AGG	0.506473	5:+46731012	MsaT027521.1:CDS
GGCTGAGGCCGAATCACCGT+TGG	0.513154	5:-46730902	None:intergenic
AAAGGGTTTCTGCTTGGCTG+AGG	0.532206	5:-46730917	None:intergenic
TTCGATTGGTAAGCATTTGG+TGG	0.535435	5:-46730974	None:intergenic
CATGATGTGTCAAACTTGGT+AGG	0.541015	5:+46730698	MsaT027521.1:CDS
TGCAACATCATCACCATGGC+TGG	0.552771	5:+46730656	None:intergenic
AATCAGAAAGCTTTATTCAA+CGG	0.559288	5:+46730805	MsaT027521.1:CDS
TAGGCATGATGTGTCAAACT+TGG	0.564079	5:+46730694	MsaT027521.1:CDS
AGCTTAAGTGATAAATCCAA+TGG	0.575565	5:-46731121	None:intergenic
CAATGTTTGCCACCACATGA+TGG	0.576640	5:+46730860	MsaT027521.1:CDS
AACTCACATCGAGTCTCACA+TGG	0.586787	5:+46730766	MsaT027521.1:CDS
ATTACTAAACTCACCAGCCA+TGG	0.594645	5:-46730669	None:intergenic
TCATGTGGTGGCAAACATTG+AGG	0.597472	5:-46730857	None:intergenic
GCATTTGCCGATAACCAACA+TGG	0.630189	5:-46730946	None:intergenic
ATGATGTGTCAAACTTGGTA+GGG	0.645237	5:+46730699	MsaT027521.1:CDS
TGTGGTGGCAAACATTGAGG+AGG	0.673338	5:-46730854	None:intergenic
GTCTCACATGGAGTACGAAG+AGG	0.675983	5:+46730778	MsaT027521.1:CDS
AAAATCTTGCCATCATGTGG+TGG	0.685328	5:-46730869	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	46730671	46731147	46730671	ID=MsaG027521
Chr5	mRNA	46730671	46731147	46730671	ID=MsaT027521.1;Parent=MsaG027521
Chr5	exon	46730671	46731147	46730671	ID=MsaT027521.1.exon1;Parent=MsaT027521.1
Chr5	CDS	46730671	46731147	46730671	ID=cds.MsaT027521.1;Parent=MsaT027521.1

Gene Sequence

>MsaT027521.1

ATGGCTGGTGAGTTTAGTAATATTAGGCATGATGTGTCAAACTTGGTAGGGTGTAAATATTGCAACCGCTTTTTCACAAACACTCAAACACTCGTAACTCACATCGAGTCTCACATGGAGTACGAAGAGGCCACAATCAGAAAGCTTTATTCAACGGAGCGTATCAACCCTAAAAGACAATTTCCTCCTCAATGTTTGCCACCACATGATGGCAAGATTTTTCAACCAAAACCAACGGTGATTCGGCCTCAGCCAAGCAGAAACCCTTTTTTCGGCCATGTTGGTTATCGGCAAATGCAACTTCCACCAAATGCTTACCAATCGAAGCACCTAGAAGGAGAATCTTCAAATGATGGTACTAAGGCATATATCAAGCAACTTGAGAAACCTATCAAGAAAATTGATTTCATTGATTTGGTTAATAATGATGATGATAACCCAGATGTTAATCCATTGGATTTATCACTTAAGCTCTAG

Protein sequence

>MsaT027521.1

MAGEFSNIRHDVSNLVGCKYCNRFFTNTQTLVTHIESHMEYEEATIRKLYSTERINPKRQFPPQCLPPHDGKIFQPKPTVIRPQPSRNPFFGHVGYRQMQLPPNAYQSKHLEGESSNDGTKAYIKQLEKPIKKIDFIDLVNNDDDNPDVNPLDLSLKL*