AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027559

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027559 MtrunA17_Chr5g0424131 97.826 414 7 2 1 413 96 508 0.0 832
MsaG027559 MtrunA17_Chr3g0114841 68.704 409 101 9 14 407 107 503 0.0 535
MsaG027559 MtrunA17_Chr8g0383981 37.192 406 217 14 8 404 120 496 7.71e-64 213
MsaG027559 MtrunA17_Chr7g0224271 35.749 414 218 14 13 405 288 674 2.52e-63 215
MsaG027559 MtrunA17_Chr7g0245601 35.121 373 217 10 32 403 443 791 5.77e-53 189
MsaG027559 MtrunA17_Chr3g0110971 34.314 408 209 15 21 403 213 586 7.95e-52 182
MsaG027559 MtrunA17_Chr4g0038761 36.088 363 202 11 56 411 110 449 2.26e-51 179
MsaG027559 MtrunA17_Chr2g0306641 33.741 409 236 14 7 406 107 489 2.90e-50 177
MsaG027559 MtrunA17_Chr1g0183641 33.251 403 235 11 6 405 107 478 5.93e-49 173
MsaG027559 MtrunA17_Chr8g0376381 32.152 395 230 14 16 403 158 521 5.23e-44 160
MsaG027559 MtrunA17_Chr5g0447691 30.591 389 242 11 24 403 174 543 6.44e-44 160
MsaG027559 MtrunA17_Chr1g0200401 31.235 413 240 12 17 406 70 461 5.52e-43 156
MsaG027559 MtrunA17_Chr3g0101141 32.329 365 221 11 48 403 194 541 1.26e-42 157
MsaG027559 MtrunA17_Chr8g0357601 31.899 395 240 12 20 403 175 551 7.18e-42 155
MsaG027559 MtrunA17_Chr4g0057891 29.567 416 261 10 3 405 125 521 1.27e-41 154
MsaG027559 MtrunA17_Chr1g0159461 31.525 387 237 14 26 403 224 591 1.54e-41 154
MsaG027559 MtrunA17_Chr2g0319091 30.673 401 249 12 14 403 196 578 3.00e-41 154
MsaG027559 MtrunA17_Chr4g0039661 31.897 348 221 7 58 403 221 554 1.39e-39 149
MsaG027559 MtrunA17_Chr3g0125811 30.052 386 244 11 26 403 202 569 1.80e-39 148
MsaG027559 MtrunA17_Chr4g0057291 30.788 406 236 17 20 406 285 664 5.45e-39 148
MsaG027559 MtrunA17_Chr4g0076091 29.692 357 226 10 58 405 204 544 5.57e-38 144
MsaG027559 MtrunA17_Chr2g0290241 26.050 357 239 9 58 405 86 426 3.84e-35 134
MsaG027559 MtrunA17_Chr5g0445331 28.827 392 249 11 24 403 158 531 4.57e-34 133
MsaG027559 MtrunA17_Chr4g0038851 26.096 456 251 14 13 403 34 468 3.44e-33 129
MsaG027559 MtrunA17_Chr2g0328391 27.612 402 252 13 19 404 291 669 7.24e-33 130
MsaG027559 MtrunA17_Chr4g0031741 28.133 391 244 15 27 404 284 650 1.49e-30 124
MsaG027559 MtrunA17_Chr7g0230771 30.334 389 236 15 24 405 372 732 6.93e-30 122
MsaG027559 MtrunA17_Chr2g0328411 27.171 357 231 9 57 403 316 653 9.18e-30 121
MsaG027559 MtrunA17_Chr4g0031711 27.924 419 259 15 5 404 339 733 2.36e-29 120
MsaG027559 MtrunA17_Chr7g0230731 29.412 391 237 15 24 405 369 729 3.73e-29 120
MsaG027559 MtrunA17_Chr2g0294461 25.926 405 264 11 16 403 199 584 1.67e-28 117
MsaG027559 MtrunA17_Chr3g0085681 24.146 410 224 9 56 403 51 435 3.99e-28 115
MsaG027559 MtrunA17_Chr4g0031701 27.114 343 209 11 5 329 232 551 4.14e-28 116
MsaG027559 MtrunA17_Chr7g0238891 24.450 409 280 10 5 403 350 739 3.97e-27 114
MsaG027559 MtrunA17_Chr2g0328431 26.536 358 234 9 57 404 316 654 7.58e-27 113
MsaG027559 MtrunA17_Chr8g0373561 29.067 375 234 13 39 405 192 542 7.78e-27 112
MsaG027559 MtrunA17_Chr4g0031731 26.291 426 258 17 5 406 232 625 2.01e-26 111
MsaG027559 MtrunA17_Chr4g0031721 26.119 402 267 12 14 404 302 684 3.94e-26 110
MsaG027559 MtrunA17_Chr2g0329591 29.697 330 196 12 3 316 42 351 4.91e-26 109
MsaG027559 MtrunA17_Chr3g0085361 25.072 347 191 7 109 403 20 349 1.25e-24 103
MsaG027559 MtrunA17_Chr5g0401131 27.681 401 243 15 26 403 113 489 2.46e-24 104
MsaG027559 MtrunA17_Chr2g0294471 24.505 404 274 12 11 403 192 575 2.46e-24 105
MsaG027559 MtrunA17_Chr5g0396491 31.760 233 135 7 14 242 43 255 4.73e-24 103
MsaG027559 MtrunA17_Chr2g0328401 27.500 280 180 8 57 329 317 580 2.18e-23 102
MsaG027559 MtrunA17_Chr2g0328381 26.098 387 255 13 27 403 275 640 2.97e-23 102
MsaG027559 MtrunA17_Chr2g0328341 24.548 387 263 11 27 404 272 638 6.28e-23 101
MsaG027559 MtrunA17_Chr7g0270031 24.627 402 263 15 16 403 178 553 1.37e-22 100
MsaG027559 MtrunA17_Chr4g0012071 25.455 385 251 14 28 405 197 552 7.50e-20 91.7
MsaG027559 MtrunA17_Chr2g0328361 24.359 390 233 15 27 403 274 614 2.97e-18 87.0
MsaG027559 MtrunA17_Chr7g0243151 26.634 413 252 18 10 403 203 583 6.92e-18 85.9
MsaG027559 MtrunA17_Chr2g0328371 24.576 236 157 7 100 329 1 221 3.51e-16 77.8
MsaG027559 MtrunA17_Chr1g0194621 22.983 409 274 13 16 406 135 520 1.90e-15 78.2
MsaG027559 MtrunA17_Chr7g0236851 27.742 310 195 10 105 403 365 656 6.78e-15 76.6
MsaG027559 MtrunA17_Chr8g0344101 23.929 397 264 14 26 403 222 599 1.39e-12 69.3
MsaG027559 MtrunA17_Chr6g0468561 26.087 391 249 15 28 403 296 661 7.65e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027559 AT4G08250.1 56.962 395 155 8 13 403 93 476 6.51e-146 424
MsaG027559 AT3G54220.1 36.070 402 223 13 13 407 279 653 4.15e-55 193
MsaG027559 AT3G03450.1 34.615 390 226 13 21 403 177 544 5.88e-53 185
MsaG027559 AT5G41920.1 35.661 401 220 14 12 403 28 399 1.02e-52 181
MsaG027559 AT5G17490.1 35.338 399 223 15 18 409 151 521 4.69e-51 179
MsaG027559 AT1G66350.1 32.737 391 221 13 21 403 149 505 5.54e-47 168
MsaG027559 AT2G01570.1 32.997 397 228 16 16 403 213 580 7.84e-45 164
MsaG027559 AT1G14920.1 31.552 393 239 13 16 403 161 528 4.86e-44 161
MsaG027559 AT5G48150.1 30.809 383 242 10 28 403 123 489 1.32e-42 156
MsaG027559 AT5G48150.4 30.809 383 242 10 28 403 123 489 1.32e-42 156
MsaG027559 AT5G48150.2 30.809 383 242 10 28 403 123 489 1.32e-42 156
MsaG027559 AT5G48150.3 30.809 383 242 10 28 403 123 489 1.32e-42 156
MsaG027559 AT4G17230.1 32.242 397 229 12 22 403 153 524 2.41e-41 153
MsaG027559 AT4G17230.2 32.242 397 229 12 22 403 153 524 2.41e-41 153
MsaG027559 AT2G04890.1 30.612 392 247 11 15 403 43 412 3.75e-41 150
MsaG027559 AT1G55580.1 32.208 385 206 12 60 403 74 444 5.20e-41 151
MsaG027559 AT1G50600.3 30.959 365 226 10 48 403 178 525 6.04e-40 149
MsaG027559 AT1G50600.2 31.233 365 225 10 48 403 253 600 1.93e-39 149
MsaG027559 AT1G50600.1 31.233 365 225 10 48 403 249 596 2.31e-39 149
MsaG027559 AT5G66770.1 30.964 394 253 10 14 403 205 583 4.19e-39 148
MsaG027559 AT3G50650.1 32.787 366 216 10 49 403 195 541 7.16e-39 147
MsaG027559 AT1G63100.2 29.512 410 245 13 17 404 267 654 1.11e-34 136
MsaG027559 AT1G63100.1 29.512 410 245 13 17 404 242 629 1.13e-34 136
MsaG027559 AT1G50420.1 28.160 451 243 14 18 405 48 480 2.20e-34 133
MsaG027559 AT1G21450.1 28.792 389 246 13 26 403 224 592 4.53e-32 128
MsaG027559 AT4G37650.1 28.283 396 252 12 27 403 146 528 2.09e-30 123
MsaG027559 AT3G60630.1 28.606 409 260 11 12 405 232 623 1.20e-29 121
MsaG027559 AT4G36710.1 29.380 371 227 13 43 403 139 484 5.82e-29 118
MsaG027559 AT2G29065.1 27.569 399 246 11 27 404 252 628 7.71e-29 119
MsaG027559 AT3G46600.2 27.027 407 229 14 26 403 3 370 1.93e-28 115
MsaG027559 AT3G46600.3 26.179 424 245 14 9 403 162 546 5.69e-28 116
MsaG027559 AT3G46600.1 26.179 424 245 14 9 403 194 578 5.84e-28 116
MsaG027559 AT1G07530.1 26.225 408 277 10 8 407 377 768 4.15e-27 114
MsaG027559 AT2G29060.2 26.316 418 263 15 5 406 269 657 5.62e-27 114
MsaG027559 AT2G29060.1 26.316 418 263 15 5 406 306 694 6.18e-27 114
MsaG027559 AT5G59450.1 26.382 398 251 12 24 403 224 597 4.59e-26 110
MsaG027559 AT2G45160.1 27.543 403 241 16 24 405 268 640 6.75e-25 107
MsaG027559 AT1G07520.2 26.196 397 253 9 27 404 154 529 1.43e-24 106
MsaG027559 AT1G07520.4 26.196 397 253 9 27 404 154 529 1.43e-24 106
MsaG027559 AT1G07520.3 26.087 391 261 8 27 404 319 694 2.05e-24 106
MsaG027559 AT1G07520.1 26.087 391 261 8 27 404 318 693 2.09e-24 106
MsaG027559 AT2G37650.2 25.707 389 260 11 24 403 332 700 1.12e-22 100
MsaG027559 AT2G37650.1 25.707 389 260 11 24 403 344 712 1.17e-22 100
MsaG027559 AT4G00150.1 27.676 383 240 12 28 407 209 557 2.01e-22 99.8
MsaG027559 AT5G52510.1 27.864 323 200 10 101 403 330 639 3.71e-17 84.0
MsaG027559 AT3G49950.1 25.000 400 263 14 26 406 29 410 1.20e-14 75.5
MsaG027559 AT3G13840.1 25.062 403 271 11 9 403 130 509 3.52e-12 68.6

Find 138 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGGTTTCCTATGAATAAA+TGG 0.181822 5:-47851095 MsaT027559.1:CDS
GACTCTTGTTTGTGCCTGTT+AGG 0.230972 5:+47851901 None:intergenic
TTGTCAAGCAAAGTTGTTAT+TGG 0.254379 5:-47850911 MsaT027559.1:CDS
CGGTTCCGTTTAGCAGAGTT+TGG 0.294952 5:+47851778 None:intergenic
GCTTGTTCGCGGGGAGGCTT+TGG 0.295883 5:-47851322 MsaT027559.1:CDS
CTTCACCAGTCTTGTACAAC+TGG 0.307827 5:+47851016 None:intergenic
TTTGCCACTGTTCAAGAAAC+TGG 0.314348 5:-47851444 MsaT027559.1:CDS
GGAAACAATAAGTTGGTGTT+AGG 0.333033 5:-47850853 MsaT027559.1:CDS
GGGGAGTGGTTGGGTGTGGA+AGG 0.333319 5:-47850967 MsaT027559.1:CDS
CCGTCTAGTGCACCTCCTTA+TGG 0.339880 5:-47851937 MsaT027559.1:CDS
TGTCAAGCAAAGTTGTTATT+GGG 0.347692 5:-47850910 MsaT027559.1:CDS
TTGAACGGTGCCAAGGAGTT+AGG 0.348949 5:-47851234 MsaT027559.1:CDS
ATTTGCCACATCTTAGTTAC+CGG 0.358640 5:-47851281 MsaT027559.1:CDS
AGGCTTTGTGGGTTTGTTCA+TGG 0.365262 5:-47851160 MsaT027559.1:CDS
GGTTGCTTTCTGCTTCTGTT+TGG 0.368542 5:-47850816 MsaT027559.1:CDS
CTCCTAGCCCATTTATTCAT+AGG 0.371100 5:+47851087 None:intergenic
GCGGCAGCGGAGGCCCTAAC+AGG 0.386266 5:-47851915 MsaT027559.1:CDS
CATAGTGGACTTTGATATCA+TGG 0.386901 5:-47851595 MsaT027559.1:CDS
ACAATAAGTTGGTGTTAGGT+TGG 0.396110 5:-47850849 MsaT027559.1:CDS
CATCAACGATGCAGGCTTTG+TGG 0.396922 5:-47851172 MsaT027559.1:CDS
GGAGCAAGAGAGGTTCTCTT+GGG 0.397324 5:-47850989 MsaT027559.1:CDS
TTTCATGAGTAACAGCTTCA+AGG 0.400010 5:+47851628 None:intergenic
ATGTATGACTCGTTCGAGGC+TGG 0.402016 5:-47851114 MsaT027559.1:CDS
AGGAGCAAGAGAGGTTCTCT+TGG 0.407648 5:-47850990 MsaT027559.1:CDS
CGTCAAGTTTGCTCATTTCA+CGG 0.410498 5:-47851664 MsaT027559.1:CDS
ATCAACGATGCAGGCTTTGT+GGG 0.413507 5:-47851171 MsaT027559.1:CDS
ACTCTTGTTTGTGCCTGTTA+GGG 0.415722 5:+47851902 None:intergenic
GGGAGTGGTTGGGTGTGGAA+GGG 0.418452 5:-47850966 MsaT027559.1:CDS
CACAAAGCCTGCATCGTTGA+TGG 0.420533 5:+47851173 None:intergenic
TGTATGACTCGTTCGAGGCT+GGG 0.428573 5:-47851113 MsaT027559.1:CDS
GGGCCTCCGCTGCCGCCATA+AGG 0.429881 5:+47851922 None:intergenic
TGCGAAGATGTGGGCCCGGC+AGG 0.434472 5:+47851520 None:intergenic
CTCCGTTGTCGAATTTGAGT+TGG 0.441756 5:+47851989 None:intergenic
CCTCCTTATGGCGGCAGCGG+AGG 0.448204 5:-47851925 MsaT027559.1:CDS
GCCACTGAGCCCATTATTCG+TGG 0.457871 5:+47851039 None:intergenic
TGGGTATAGAGTGGAAGAGT+TGG 0.459148 5:-47850875 MsaT027559.1:CDS
TCGCGAAAAGAAGGCCTGCC+GGG 0.460380 5:-47851534 MsaT027559.1:CDS
GGAGGAGGAGGATGACAGAA+AGG 0.460604 5:-47851964 MsaT027559.1:CDS
GGGTATAGAGTGGAAGAGTT+GGG 0.462213 5:-47850874 MsaT027559.1:CDS
CTTCGTTGAAGCTTGTTCGC+GGG 0.462944 5:-47851332 MsaT027559.1:CDS
AAGCTGTTACTCATGAAAGA+AGG 0.464529 5:-47851623 MsaT027559.1:CDS
TAACAGCTTCAAGGATGGCT+TGG 0.472218 5:+47851637 None:intergenic
TTCGACAACGGAGGAGGAGG+AGG 0.476647 5:-47851979 MsaT027559.1:CDS
ACATTAGTCGAGGAGGAAGT+AGG 0.480496 5:-47851198 MsaT027559.1:CDS
GAGCTTTGTTCCTAACTCCT+TGG 0.485022 5:+47851224 None:intergenic
GCCCTCCAAACTCTGCTAAA+CGG 0.486218 5:-47851783 MsaT027559.1:CDS
TGGCTCAAGTGATATTGATT+CGG 0.487459 5:-47851872 MsaT027559.1:CDS
GCACCTCCTTATGGCGGCAG+CGG 0.487716 5:-47851928 MsaT027559.1:CDS
TGAGAGTATGTCTGTTTGGT+GGG 0.489093 5:+47851719 None:intergenic
ATCGCGAAAAGAAGGCCTGC+CGG 0.491290 5:-47851535 MsaT027559.1:CDS
TCACTTTCATCGCGAAAAGA+AGG 0.496428 5:-47851543 MsaT027559.1:CDS
TATTGGGTTTATTTAACGAT+GGG 0.498402 5:-47850894 MsaT027559.1:CDS
CAGTGAGAAGACAGAGTTTG+TGG 0.500911 5:+47851745 None:intergenic
CCTCCGCTGCCGCCATAAGG+AGG 0.504163 5:+47851925 None:intergenic
CTCAAATTCGACAACGGAGG+AGG 0.505265 5:-47851985 MsaT027559.1:CDS
AGGCTGAGAGTATGTCTGTT+TGG 0.507765 5:+47851715 None:intergenic
TGGGTTTCCTATGAATAAAT+GGG 0.510723 5:-47851094 MsaT027559.1:CDS
AGTGATATTGATTCGGCTCA+AGG 0.514796 5:-47851865 MsaT027559.1:CDS
CACAAACAAGAGTCACCATC+TGG 0.516410 5:-47851892 MsaT027559.1:CDS
TCCGTTTAGCAGAGTTTGGA+GGG 0.520521 5:+47851782 None:intergenic
GACAACGGAGGAGGAGGAGG+AGG 0.522162 5:-47851976 MsaT027559.1:CDS
TTGGGTGTGGAAGGGTTCAA+AGG 0.525480 5:-47850958 MsaT027559.1:CDS
TTCCGTTTAGCAGAGTTTGG+AGG 0.528085 5:+47851781 None:intergenic
ATAACAACTTTGCTTGACAA+TGG 0.528206 5:+47850914 None:intergenic
CACAAACTCTGTCTTCTCAC+TGG 0.528238 5:-47851744 MsaT027559.1:CDS
ACAAAGCCTGCATCGTTGAT+GGG 0.528462 5:+47851174 None:intergenic
TGAAAGAAGGGTTCACATAG+TGG 0.529945 5:-47851610 MsaT027559.1:CDS
TGAGTTGGACATATCTCCCA+TGG 0.532180 5:+47852004 None:intergenic
GTAGGGCCCATCAACGATGC+AGG 0.535058 5:-47851180 MsaT027559.1:CDS
TAAATGGGCTAGGAGTTTAG+TGG 0.535874 5:-47851079 MsaT027559.1:CDS
AAAGGAGTTAACATAAGCTA+TGG 0.536826 5:-47850940 MsaT027559.1:CDS
TCTTCCAGTTTCTTGAACAG+TGG 0.536983 5:+47851440 None:intergenic
TTATTGGGTTTATTTAACGA+TGG 0.539610 5:-47850895 MsaT027559.1:CDS
CTGAGAGTATGTCTGTTTGG+TGG 0.539651 5:+47851718 None:intergenic
AGCGATGTATGACTCGTTCG+AGG 0.539838 5:-47851118 MsaT027559.1:CDS
GAGGAGGAGGATGACAGAAA+GGG 0.544247 5:-47851963 MsaT027559.1:CDS
AGTTTCTTGAACAGTGGCAA+AGG 0.546696 5:+47851446 None:intergenic
TTCCTATGAATAAATGGGCT+AGG 0.550588 5:-47851089 MsaT027559.1:CDS
GTGAAGAGGAGGAGCAAGAG+AGG 0.551124 5:-47850999 MsaT027559.1:CDS
TCTATTGCTTCATTCTTGAA+CGG 0.552156 5:-47851249 MsaT027559.1:CDS
CTCCATGTTGGTGCCGTGAG+TGG 0.552588 5:+47851830 None:intergenic
ATTGAATGAGTGAAGCCCAC+TGG 0.554626 5:+47851565 None:intergenic
TTCATTCTTGAACGGTGCCA+AGG 0.558389 5:-47851241 MsaT027559.1:CDS
CATTAGTCGAGGAGGAAGTA+GGG 0.559643 5:-47851197 MsaT027559.1:CDS
TCTTCGTTGAAGCTTGTTCG+CGG 0.561338 5:-47851333 MsaT027559.1:CDS
AGAGTTGGGAAACAATAAGT+TGG 0.563779 5:-47850860 MsaT027559.1:CDS
GACTTAGTGTCCTCCACTCA+CGG 0.564053 5:-47851843 MsaT027559.1:CDS
ATTTAACGATGGGTATAGAG+TGG 0.564807 5:-47850884 MsaT027559.1:CDS
AGCTGTTACTCATGAAAGAA+GGG 0.566179 5:-47851622 MsaT027559.1:CDS
GTGGCCCAGTTGTACAAGAC+TGG 0.567811 5:-47851021 MsaT027559.1:CDS
AAATTCGACAACGGAGGAGG+AGG 0.568207 5:-47851982 MsaT027559.1:CDS
TTCGTTGAAGCTTGTTCGCG+GGG 0.568750 5:-47851331 MsaT027559.1:CDS
TTTGCCACATCTTAGTTACC+GGG 0.572604 5:-47851280 MsaT027559.1:CDS
GCAAGAGAGGTTCTCTTGGG+GGG 0.573233 5:-47850986 MsaT027559.1:CDS
GTGGACTTTGATATCATGGA+AGG 0.574432 5:-47851591 MsaT027559.1:CDS
AAGCAATAGAATCTGAAGCC+CGG 0.574832 5:+47851262 None:intergenic
CATGTCTTGAAGAAGCTGAA+AGG 0.576740 5:+47851695 None:intergenic
CTACATTGATGAAAAGTGAA+TGG 0.578567 5:+47851384 None:intergenic
ATGAGTAACAGCTTCAAGGA+TGG 0.578715 5:+47851632 None:intergenic
AAATGAGCAAACTTGACGTA+AGG 0.579384 5:+47851669 None:intergenic
CCACTGAGCCCATTATTCGT+GGG 0.580098 5:+47851040 None:intergenic
GGACTTTGATATCATGGAAG+GGG 0.580763 5:-47851589 MsaT027559.1:CDS
AGAAACAAAGAACGTGGCAA+TGG 0.585187 5:-47851483 MsaT027559.1:CDS
CTCCACTCACGGCACCAACA+TGG 0.585629 5:-47851832 MsaT027559.1:CDS
GTGATGCGAAGATGTGGGCC+CGG 0.587438 5:+47851516 None:intergenic
CTGTTCAAGAAACTGGAAGA+AGG 0.593746 5:-47851437 MsaT027559.1:CDS
GTTGTACAAGACTGGTGAAG+AGG 0.596068 5:-47851013 MsaT027559.1:CDS
TATCATGGAAGGGGCCCAGT+GGG 0.596303 5:-47851580 MsaT027559.1:CDS
GTTGAAGCTTGTTCGCGGGG+AGG 0.596988 5:-47851328 MsaT027559.1:CDS
CTGCTAAACGGAACCGACTG+TGG 0.597512 5:-47851771 MsaT027559.1:CDS
GTACAAGACTGGTGAAGAGG+AGG 0.599329 5:-47851010 MsaT027559.1:CDS
TCAATATCACTTGAGCCAGA+TGG 0.599918 5:+47851877 None:intergenic
ATGGGCTAGGAGTTTAGTGG+AGG 0.599983 5:-47851076 MsaT027559.1:CDS
TTGAATGAGTGAAGCCCACT+GGG 0.601535 5:+47851566 None:intergenic
CCATAAGGAGGTGCACTAGA+CGG 0.601550 5:+47851937 None:intergenic
TAGCAGAGTTTGGAGGGCGT+CGG 0.602737 5:+47851788 None:intergenic
GAGTTTGTGGTGACCACAGT+CGG 0.604569 5:+47851758 None:intergenic
GAGCAAGAGAGGTTCTCTTG+GGG 0.606683 5:-47850988 MsaT027559.1:CDS
AATGCAGTGATGCGAAGATG+TGG 0.609707 5:+47851510 None:intergenic
CATGTTGGTGCCGTGAGTGG+AGG 0.611646 5:+47851833 None:intergenic
TGGACTTTGATATCATGGAA+GGG 0.613948 5:-47851590 MsaT027559.1:CDS
CGCAGCGAGTCTCTCCATGT+TGG 0.618231 5:+47851818 None:intergenic
TGTTAGGTTGGAAATCAAGA+CGG 0.623526 5:-47850837 MsaT027559.1:CDS
TTCACCAGTCTTGTACAACT+GGG 0.634954 5:+47851017 None:intergenic
AAAAGTGAATGGTTGTCCAA+CGG 0.637307 5:+47851395 None:intergenic
AGCAAGAGAGGTTCTCTTGG+GGG 0.646650 5:-47850987 MsaT027559.1:CDS
GAAGCCCGGTAACTAAGATG+TGG 0.661574 5:+47851276 None:intergenic
GAGAGTATGTCTGTTTGGTG+GGG 0.663577 5:+47851720 None:intergenic
GCTCGTGACATTAGTCGAGG+AGG 0.670792 5:-47851205 MsaT027559.1:CDS
CCCACGAATAATGGGCTCAG+TGG 0.676277 5:-47851040 MsaT027559.1:CDS
AAAGCTCGTGACATTAGTCG+AGG 0.678929 5:-47851208 MsaT027559.1:CDS
ATGCAGTGATGCGAAGATGT+GGG 0.688648 5:+47851511 None:intergenic
GTCCAACTCAAATTCGACAA+CGG 0.695660 5:-47851991 MsaT027559.1:CDS
TAAGGAGGTGCACTAGACGG+AGG 0.697542 5:+47851940 None:intergenic
ATATCATGGAAGGGGCCCAG+TGG 0.698302 5:-47851581 MsaT027559.1:CDS
TCTAGTGCACCTCCTTATGG+CGG 0.711985 5:-47851934 MsaT027559.1:CDS
CAACTCAAATTCGACAACGG+AGG 0.712986 5:-47851988 MsaT027559.1:CDS
TTGTCTAGAAACAAAGAACG+TGG 0.726054 5:-47851489 MsaT027559.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 47850783 47852024 47850783 ID=MsaG027559
Chr5 mRNA 47850783 47852024 47850783 ID=MsaT027559.1;Parent=MsaG027559
Chr5 exon 47850783 47852024 47850783 ID=MsaT027559.1.exon1;Parent=MsaT027559.1
Chr5 CDS 47850783 47852024 47850783 ID=cds.MsaT027559.1;Parent=MsaT027559.1
Gene Sequence

>MsaT027559.1

ATGGGAGATATGTCCAACTCAAATTCGACAACGGAGGAGGAGGAGGAGGATGACAGAAAGGGCCTCCGTCTAGTGCACCTCCTTATGGCGGCAGCGGAGGCCCTAACAGGCACAAACAAGAGTCACCATCTGGCTCAAGTGATATTGATTCGGCTCAAGGACTTAGTGTCCTCCACTCACGGCACCAACATGGAGAGACTCGCTGCGTATTTTACCGACGCCCTCCAAACTCTGCTAAACGGAACCGACTGTGGTCACCACAAACTCTGTCTTCTCACTGGACCCCACCAAACAGACATACTCTCAGCCTTTCAGCTTCTTCAAGACATGTCTCCTTACGTCAAGTTTGCTCATTTCACGGCAAACCAAGCCATCCTTGAAGCTGTTACTCATGAAAGAAGGGTTCACATAGTGGACTTTGATATCATGGAAGGGGCCCAGTGGGCTTCACTCATTCAATCACTTTCATCGCGAAAAGAAGGCCTGCCGGGCCCACATCTTCGCATCACTGCATTGTCTAGAAACAAAGAACGTGGCAATGGACGATCTAGATCGTCCTTTGCCACTGTTCAAGAAACTGGAAGAAGGTTAACAACTTTTGCTGCTTCCGTTGGACAACCATTCACTTTTCATCAATGTAGGTTGGAATCAGACGAAACTTTTCGAACATCTTCGTTGAAGCTTGTTCGCGGGGAGGCTTTGGTGTTCAACTGTGTCATGCATTTGCCACATCTTAGTTACCGGGCTTCAGATTCTATTGCTTCATTCTTGAACGGTGCCAAGGAGTTAGGAACAAAGCTCGTGACATTAGTCGAGGAGGAAGTAGGGCCCATCAACGATGCAGGCTTTGTGGGTTTGTTCATGGATTCATTGCATCGTTATTCAGCGATGTATGACTCGTTCGAGGCTGGGTTTCCTATGAATAAATGGGCTAGGAGTTTAGTGGAGGAAGTATTTTTAGGCCCACGAATAATGGGCTCAGTGGCCCAGTTGTACAAGACTGGTGAAGAGGAGGAGCAAGAGAGGTTCTCTTGGGGGGAGTGGTTGGGTGTGGAAGGGTTCAAAGGAGTTAACATAAGCTATGGTAACCATTGTCAAGCAAAGTTGTTATTGGGTTTATTTAACGATGGGTATAGAGTGGAAGAGTTGGGAAACAATAAGTTGGTGTTAGGTTGGAAATCAAGACGGTTGCTTTCTGCTTCTGTTTGGACTTGTTTGTCAGAGATTGAATATGATTTTTAA
Protein sequence

>MsaT027559.1

MGDMSNSNSTTEEEEEDDRKGLRLVHLLMAAAEALTGTNKSHHLAQVILIRLKDLVSSTHGTNMERLAAYFTDALQTLLNGTDCGHHKLCLLTGPHQTDILSAFQLLQDMSPYVKFAHFTANQAILEAVTHERRVHIVDFDIMEGAQWASLIQSLSSRKEGLPGPHLRITALSRNKERGNGRSRSSFATVQETGRRLTTFAASVGQPFTFHQCRLESDETFRTSSLKLVRGEALVFNCVMHLPHLSYRASDSIASFLNGAKELGTKLVTLVEEEVGPINDAGFVGLFMDSLHRYSAMYDSFEAGFPMNKWARSLVEEVFLGPRIMGSVAQLYKTGEEEEQERFSWGEWLGVEGFKGVNISYGNHCQAKLLLGLFNDGYRVEELGNNKLVLGWKSRRLLSASVWTCLSEIEYDF*