AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027621


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027621 MtrunA17_Chr5g0424861 96.379 359 12 1 16 373 1 359 0.0 719
MsaG027621 MtrunA17_Chr3g0115371 65.143 350 114 4 16 361 1 346 1.05e-157 447
MsaG027621 MtrunA17_Chr5g0424851 80.603 232 44 1 141 371 1 232 3.35e-131 382
MsaG027621 MtrunA17_Chr5g0424851 74.882 211 53 0 146 356 220 430 9.68e-109 325
MsaG027621 MtrunA17_Chr3g0124601 49.587 363 172 3 4 361 2 358 2.71e-117 344
MsaG027621 MtrunA17_Chr8g0382861 47.126 348 177 4 14 361 13 353 2.23e-99 298
MsaG027621 MtrunA17_Chr8g0353501 51.568 287 128 2 82 361 181 463 1.26e-89 277
MsaG027621 MtrunA17_Chr7g0256211 50.871 287 130 3 82 361 167 449 7.33e-89 275
MsaG027621 MtrunA17_Chr2g0321431 51.429 280 129 2 82 361 194 466 7.59e-89 276
MsaG027621 MtrunA17_Chr7g0258321 47.020 302 149 3 69 361 161 460 4.79e-88 273
MsaG027621 MtrunA17_Chr1g0174701 42.037 383 196 7 4 361 122 503 1.60e-85 268
MsaG027621 MtrunA17_Chr4g0026401 45.981 311 155 5 52 361 160 458 1.89e-83 262
MsaG027621 MtrunA17_Chr5g0422771 44.631 298 154 2 71 361 168 461 4.01e-78 248
MsaG027621 MtrunA17_Chr1g0210151 46.367 289 149 3 77 360 49 336 2.90e-77 241
MsaG027621 MtrunA17_Chr3g0093451 43.827 162 84 3 84 238 48 209 1.76e-40 142
MsaG027621 MtrunA17_Chr1g0196951 31.000 200 115 5 163 357 59 240 3.49e-15 74.3
MsaG027621 MtrunA17_Chr7g0242651 25.698 179 117 3 188 354 45 219 2.27e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027621 AT1G77920.1 52.785 377 148 6 1 361 1 363 6.79e-123 359
MsaG027621 AT1G22070.1 50.538 372 159 4 14 365 17 383 8.01e-118 347
MsaG027621 AT5G10030.5 50.416 361 173 3 4 361 2 359 8.59e-116 341
MsaG027621 AT5G10030.3 50.416 361 173 3 4 361 2 359 8.59e-116 341
MsaG027621 AT5G10030.1 50.416 361 173 3 4 361 2 359 8.59e-116 341
MsaG027621 AT5G10030.4 50.416 361 173 3 4 361 2 359 8.59e-116 341
MsaG027621 AT5G10030.2 50.416 361 173 3 4 361 2 359 8.59e-116 341
MsaG027621 AT5G65210.5 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.7 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.3 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.1 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.2 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.4 48.919 370 182 4 4 369 2 368 3.07e-115 340
MsaG027621 AT5G65210.6 53.512 299 135 2 72 369 3 298 3.82e-105 311
MsaG027621 AT1G08320.2 47.095 327 154 6 46 361 8 326 2.03e-91 278
MsaG027621 AT1G08320.5 47.095 327 154 6 46 361 8 326 2.03e-91 278
MsaG027621 AT1G08320.1 47.095 327 154 6 46 361 132 450 6.47e-90 279
MsaG027621 AT1G08320.4 47.095 327 154 6 46 361 132 450 6.47e-90 279
MsaG027621 AT1G08320.3 47.095 327 154 6 46 361 132 450 6.47e-90 279
MsaG027621 AT5G06839.1 45.322 342 166 5 37 361 75 412 6.23e-86 266
MsaG027621 AT5G06950.2 48.951 286 136 2 82 361 46 327 7.05e-86 263
MsaG027621 AT5G06950.4 48.951 286 136 2 82 361 46 327 7.05e-86 263
MsaG027621 AT5G06950.1 48.951 286 136 2 82 361 46 327 7.05e-86 263
MsaG027621 AT5G06950.5 48.951 286 136 2 82 361 46 327 7.05e-86 263
MsaG027621 AT5G06950.3 48.951 286 136 2 82 361 46 327 7.05e-86 263
MsaG027621 AT5G06839.3 47.213 305 146 2 72 361 151 455 1.33e-85 267
MsaG027621 AT5G06839.2 44.606 343 168 5 37 361 75 413 9.09e-85 263
MsaG027621 AT3G12250.5 48.601 286 137 2 82 361 19 300 2.20e-84 259
MsaG027621 AT3G12250.6 48.601 286 137 2 82 361 46 327 3.91e-84 259
MsaG027621 AT3G12250.2 48.601 286 137 2 82 361 46 327 3.91e-84 259
MsaG027621 AT3G12250.7 48.601 286 137 2 82 361 46 327 3.91e-84 259
MsaG027621 AT3G12250.1 48.601 286 137 2 82 361 46 327 3.91e-84 259
MsaG027621 AT3G12250.3 48.601 286 137 2 82 361 40 321 4.95e-84 258
MsaG027621 AT3G12250.4 48.601 286 137 2 82 361 71 352 1.98e-83 258
MsaG027621 AT5G06960.2 48.252 286 138 2 82 361 46 327 5.48e-83 256
MsaG027621 AT5G06960.1 48.252 286 138 2 82 361 46 327 5.48e-83 256
MsaG027621 AT5G06960.3 48.252 286 138 2 82 361 26 307 5.68e-83 255
MsaG027621 AT1G68640.1 44.631 298 153 3 72 361 156 449 1.60e-72 233
MsaG027621 AT1G58330.1 29.381 194 114 6 178 354 36 223 1.94e-12 66.6

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGGATGTCGGTTATA+TGG 0.184139 5:-49000826 MsaT027621.1:CDS
TCTTTGTTTGAAATTGAATA+TGG 0.229069 5:-49000696 MsaT027621.1:CDS
CAAGCTGATCCCTTGGATTT+CGG 0.267505 5:-48999873 MsaT027621.1:CDS
TTTATACAGAAACAGTTTAT+TGG 0.277561 5:-49000841 MsaT027621.1:CDS
TGCTCAAGGCTTGTTGGCTA+TGG 0.316587 5:-48999541 MsaT027621.1:CDS
ATCCTCCTATCCAAAGAAAA+AGG 0.354680 5:+49000475 None:intergenic
TTATGTTCATCTTCAACATT+GGG 0.356119 5:+49001748 None:intergenic
TCATTCAAATTCTCAAGTTT+CGG 0.363992 5:+49000002 None:intergenic
GCTGAAGATGCACTGTCAAT+AGG 0.369583 5:-48999933 MsaT027621.1:CDS
CTTATGTTCATCTTCAACAT+TGG 0.379233 5:+49001747 None:intergenic
GCTCAAGGCTTGTTGGCTAT+GGG 0.379556 5:-48999540 MsaT027621.1:CDS
CTAACCTGAGTTTATTGTTC+CGG 0.383626 5:+49000797 None:intergenic
CTCAACTAATCGCGCCGAAA+AGG 0.387136 5:-49001857 MsaT027621.1:CDS
TCAAGCTGCTCAAGGCTTGT+TGG 0.395796 5:-48999547 MsaT027621.1:CDS
ACAGGCTTATGTTCAACAAC+TGG 0.406023 5:-49001026 MsaT027621.1:intron
CTTTCAGATGGCTGCTGCCA+TGG 0.412683 5:-48999841 MsaT027621.1:CDS
TATCGAGAAAACAAGAAAAC+AGG 0.417815 5:-49000966 MsaT027621.1:intron
CTTTGCAGGCAGATCACCTT+AGG 0.420677 5:-48999611 MsaT027621.1:intron
TTATTGGATGTCGGTTATAT+GGG 0.423086 5:-49000825 MsaT027621.1:CDS
CCTCCCACATAGTCACTTGA+AGG 0.428980 5:+49001814 None:intergenic
AAGGTTCTCTTGATTCTTGA+GGG 0.435161 5:+49001599 None:intergenic
CCCTTGGATTTCGGAAACTA+CGG 0.436857 5:-48999864 MsaT027621.1:CDS
CAAGAATCAAGAGAACCTTC+TGG 0.437311 5:-49001595 MsaT027621.1:CDS
TACTGCTTCTCCTTTGTCCA+TGG 0.443791 5:+48999824 None:intergenic
CTGAAGATGCACTGTCAATA+GGG 0.444666 5:-48999932 MsaT027621.1:CDS
GACAAAGGAGAAGCAGTAGA+AGG 0.456711 5:-48999819 MsaT027621.1:CDS
AGTGTTGAGTAGGGTAGAAA+TGG 0.460652 5:+48999460 None:intergenic
CAACATTCAAGCTGATCCCT+TGG 0.462368 5:-48999880 MsaT027621.1:CDS
TCTTAATTTGAGTGTTGAGT+AGG 0.465347 5:+48999450 None:intergenic
GTCGGTTATATGGGAACGTC+CGG 0.469360 5:-49000816 MsaT027621.1:CDS
GAAGGTTCTCTTGATTCTTG+AGG 0.474792 5:+49001598 None:intergenic
TGTTGTTGATCATACTCTGC+TGG 0.478454 5:+48999898 None:intergenic
TCTATTTAATCTCTGGCGTA+TGG 0.481044 5:-49000515 MsaT027621.1:CDS
ATGTCACGCATCTTATCAAT+TGG 0.485700 5:-48999570 MsaT027621.1:CDS
CTCAAGGCTTGTTGGCTATG+GGG 0.490238 5:-48999539 MsaT027621.1:CDS
CTTAATTTGAGTGTTGAGTA+GGG 0.496496 5:+48999451 None:intergenic
CATAAGTAGCATGGAGAACA+AGG 0.509417 5:-49001730 MsaT027621.1:intron
CACATAGTCACTTGAAGGAA+TGG 0.522645 5:+49001819 None:intergenic
CATTCCTTCAAGTGACTATG+TGG 0.523116 5:-49001818 MsaT027621.1:CDS
TCAAGGCTTGTTGGCTATGG+GGG 0.523504 5:-48999538 MsaT027621.1:CDS
AGATGCACTGTCAATAGGGT+TGG 0.527361 5:-48999928 MsaT027621.1:CDS
ATAACAGAGTTTGTTGCCTA+AGG 0.529419 5:+48999595 None:intergenic
TTGAAATTGAATATGGACGT+TGG 0.536052 5:-49000689 MsaT027621.1:CDS
TCAATTGGTCAAGCTGCTCA+AGG 0.536456 5:-48999555 MsaT027621.1:CDS
TTCGCACTCTTAGTTCATTG+TGG 0.540430 5:-48999500 MsaT027621.1:CDS
TATTCAAGAACCTGTTCCTA+AGG 0.542554 5:-49001557 MsaT027621.1:intron
TAGATTGAAGCTCATGCAGT+TGG 0.542577 5:-49000996 MsaT027621.1:CDS
CGGAAACTACGGCTTTCAGA+TGG 0.543160 5:-48999853 MsaT027621.1:CDS
AATGAAAGCAGAAGCTGCAA+AGG 0.554661 5:-49000547 MsaT027621.1:CDS
AGAAACAGTTTATTGGATGT+CGG 0.555658 5:-49000834 MsaT027621.1:CDS
GCCGTAGTTTCCGAAATCCA+AGG 0.586415 5:+48999863 None:intergenic
GAAGATGAACATAAGTAGCA+TGG 0.592197 5:-49001739 MsaT027621.1:CDS
TGTACATTTAGAATTTGTGA+TGG 0.598170 5:+49000447 None:intergenic
GCGCGGCTGCTCGAAAATGT+CGG 0.599361 5:-49001130 MsaT027621.1:CDS
ATGGCTGCTGCCATGGACAA+AGG 0.602885 5:-48999834 MsaT027621.1:CDS
GTCTCTTGATCATCTCCAGA+AGG 0.603267 5:+49001580 None:intergenic
CTCTGGCGTATGGAAATCAC+CGG 0.616184 5:-49000505 MsaT027621.1:CDS
TTCATCTACTTGTCGAGAGT+AGG 0.630773 5:-49000596 MsaT027621.1:CDS
CCGTAGTTTCCGAAATCCAA+GGG 0.636532 5:+48999864 None:intergenic
CCTTCAAGTGACTATGTGGG+AGG 0.650193 5:-49001814 MsaT027621.1:CDS
ATTCCTTCAAGTGACTATGT+GGG 0.664208 5:-49001817 MsaT027621.1:CDS
ACGTCCGGAACAATAAACTC+AGG 0.666698 5:-49000801 MsaT027621.1:intron
CTTGCTGAGATGATAAACGC+AGG 0.683767 5:+48999955 None:intergenic
TATGTTCATCTTCAACATTG+GGG 0.698417 5:+49001749 None:intergenic
ACGTCAAGCACAGAATCGCG+CGG 0.719929 5:-49001147 MsaT027621.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 48999452 49001901 48999452 ID=MsaG027621
Chr5 mRNA 48999452 49001901 48999452 ID=MsaT027621.1;Parent=MsaG027621
Chr5 exon 48999452 48999625 48999452 ID=MsaT027621.1.exon8;Parent=MsaT027621.1
Chr5 CDS 48999452 48999625 48999452 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 48999806 49000033 48999806 ID=MsaT027621.1.exon7;Parent=MsaT027621.1
Chr5 CDS 48999806 49000033 48999806 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49000452 49000723 49000452 ID=MsaT027621.1.exon6;Parent=MsaT027621.1
Chr5 CDS 49000452 49000723 49000452 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49000802 49000865 49000802 ID=MsaT027621.1.exon5;Parent=MsaT027621.1
Chr5 CDS 49000802 49000865 49000802 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49000967 49001044 49000967 ID=MsaT027621.1.exon4;Parent=MsaT027621.1
Chr5 CDS 49000967 49001044 49000967 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49001118 49001177 49001118 ID=MsaT027621.1.exon3;Parent=MsaT027621.1
Chr5 CDS 49001118 49001177 49001118 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49001558 49001632 49001558 ID=MsaT027621.1.exon2;Parent=MsaT027621.1
Chr5 CDS 49001558 49001632 49001558 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Chr5 exon 49001731 49001901 49001731 ID=MsaT027621.1.exon1;Parent=MsaT027621.1
Chr5 CDS 49001731 49001901 49001731 ID=cds.MsaT027621.1;Parent=MsaT027621.1
Gene Sequence

>MsaT027621.1

ATGAACTCTTCTTCATCATCAACTCAACTAATCGCGCCGAAAAGGATGAACATGTATGAGCCATTCCTTCAAGTGACTATGTGGGAGGATAGCTTTAAAGTTGATCATAGTACTTTGAATTCAATTGCTTCCCCAATGTTGAAGATGAACATAAGTAGCATGGAGAACAAGTCTGAATGTATCCCTCAAGAATCAAGAGAACCTTCTGGAGATGATCAAGAGACTATTCAAGAACCTGTTCCTAAGGTACTACGACGTCAAGCACAGAATCGCGCGGCTGCTCGAAAATGTCGGCTGCGAAAAAAGGCTTATGTTCAACAACTGGAAACGAGTAGATTGAAGCTCATGCAGTTGGAATTAGATATCGAGAAAACAAGAAAACAGGGTTTATACAGAAACAGTTTATTGGATGTCGGTTATATGGGAACGTCCGGAACAATAAACTCAGGGATATCTTTGTTTGAAATTGAATATGGACGTTGGATTGAAGAACAAGATCAACAAAACGAAGAACTAAGAAATGCATTGCAAACTAATGCATCTGATATACATCTTCATCTACTTGTCGAGAGTAGGTTGAACCAATACTCAAATCTTTTTCAAATGAAAGCAGAAGCTGCAAAGGCTGATGTTTTCTATTTAATCTCTGGCGTATGGAAATCACCGGTCGAACGCCTTTTTCTTTGGATAGGAGGATCTCGTCCATCACAAATTCTAAATATCGTGGTACCGAAACTTGAGAATTTGAATGATCAACAAATTGCTAGCATTAACAACCTGCGTTTATCATCTCAGCAAGCTGAAGATGCACTGTCAATAGGGTTGGAGAAACTCCAGCAGAGTATGATCAACAACATTCAAGCTGATCCCTTGGATTTCGGAAACTACGGCTTTCAGATGGCTGCTGCCATGGACAAAGGAGAAGCAGTAGAAGGTTTTGTAATTCAGGCAGATCACCTTAGGCAACAAACTCTGTTATACATGTCACGCATCTTATCAATTGGTCAAGCTGCTCAAGGCTTGTTGGCTATGGGGGAATACTTTCATCGTCTTCGCACTCTTAGTTCATTGTGGACTGCTCGTTCATGTCACCATTTCTACCCTACTCAACACTCAAATTAA

Protein sequence

>MsaT027621.1

MNSSSSSTQLIAPKRMNMYEPFLQVTMWEDSFKVDHSTLNSIASPMLKMNISSMENKSECIPQESREPSGDDQETIQEPVPKVLRRQAQNRAAARKCRLRKKAYVQQLETSRLKLMQLELDIEKTRKQGLYRNSLLDVGYMGTSGTINSGISLFEIEYGRWIEEQDQQNEELRNALQTNASDIHLHLLVESRLNQYSNLFQMKAEAAKADVFYLISGVWKSPVERLFLWIGGSRPSQILNIVVPKLENLNDQQIASINNLRLSSQQAEDALSIGLEKLQQSMINNIQADPLDFGNYGFQMAAAMDKGEAVEGFVIQADHLRQQTLLYMSRILSIGQAAQGLLAMGEYFHRLRTLSSLWTARSCHHFYPTQHSN*