Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Gene ID | Type | Classification |
---|---|---|
MsaG027627 | TF | bZIP |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000006 | MsaG027627 | 0.805961 | 1.755619e-49 | 2.721910e-47 |
MsaG000236 | MsaG027627 | 0.810446 | 1.964609e-50 | 3.391767e-48 |
MsaG001169 | MsaG027627 | 0.807667 | 7.681568e-50 | 1.240095e-47 |
MsaG002286 | MsaG027627 | 0.806022 | 1.704260e-49 | 2.646135e-47 |
MsaG002350 | MsaG027627 | 0.852216 | 1.022709e-60 | 5.986041e-58 |
MsaG003912 | MsaG027627 | 0.809346 | 3.379541e-50 | 5.680558e-48 |
MsaG004201 | MsaG027627 | 0.808334 | 5.548962e-50 | 9.102831e-48 |
MsaG004361 | MsaG027627 | 0.848757 | 9.479186e-60 | 4.924166e-57 |
MsaG004427 | MsaG027627 | 0.824918 | 1.103026e-53 | 2.768535e-51 |
MsaG004431 | MsaG027627 | 0.823222 | 2.747215e-53 | 6.584232e-51 |
MsaG005051 | MsaG027627 | 0.813158 | 5.078772e-51 | 9.373898e-49 |
MsaG005217 | MsaG027627 | 0.813318 | 4.686708e-51 | 8.684778e-49 |
MsaG005334 | MsaG027627 | 0.860114 | 5.086930e-63 | 3.972591e-60 |
MsaG006083 | MsaG027627 | 0.804981 | 2.811340e-49 | 4.260237e-47 |
MsaG006440 | MsaG027627 | 0.800798 | 2.036477e-48 | 2.803577e-46 |
MsaG006517 | MsaG027627 | 0.806850 | 1.142519e-49 | 1.808952e-47 |
MsaG006630 | MsaG027627 | 0.817255 | 6.305562e-52 | 1.291162e-49 |
MsaG006755 | MsaG027627 | 0.818877 | 2.721376e-52 | 5.810804e-50 |
MsaG006792 | MsaG027627 | 0.807054 | 1.034810e-49 | 1.646338e-47 |
MsaG006798 | MsaG027627 | 0.810603 | 1.817590e-50 | 3.149859e-48 |
MsaG006884 | MsaG027627 | 0.847177 | 2.573253e-59 | 1.267594e-56 |
MsaG006886 | MsaG027627 | 0.825285 | 9.043731e-54 | 2.292813e-51 |
MsaG007072 | MsaG027627 | 0.804653 | 3.289634e-49 | 4.947184e-47 |
MsaG007480 | MsaG027627 | 0.806203 | 1.561693e-49 | 2.435330e-47 |
MsaG008069 | MsaG027627 | 0.813029 | 5.419086e-51 | 9.970057e-49 |
MsaG008286 | MsaG027627 | 0.815276 | 1.738687e-51 | 3.384594e-49 |
MsaG008372 | MsaG027627 | 0.817543 | 5.434381e-52 | 1.121011e-49 |
MsaG008592 | MsaG027627 | 0.814153 | 3.074475e-51 | 5.817814e-49 |
MsaG008604 | MsaG027627 | 0.813998 | 3.324423e-51 | 6.266511e-49 |
MsaG008815 | MsaG027627 | 0.838195 | 6.105595e-57 | 2.252790e-54 |
MsaG008880 | MsaG027627 | 0.815975 | 1.217077e-51 | 2.411571e-49 |
MsaG009009 | MsaG027627 | 0.802721 | 8.243915e-49 | 1.185594e-46 |
MsaG009261 | MsaG027627 | 0.811210 | 1.345078e-50 | 2.365856e-48 |
MsaG009269 | MsaG027627 | 0.812013 | 9.016448e-51 | 1.617460e-48 |
MsaG009392 | MsaG027627 | 0.818171 | 3.928215e-52 | 8.235363e-50 |
MsaG009477 | MsaG027627 | 0.813186 | 5.007493e-51 | 9.248964e-49 |
MsaG009764 | MsaG027627 | 0.801085 | 1.781137e-48 | 2.467821e-46 |
MsaG009787 | MsaG027627 | 0.811313 | 1.277627e-50 | 2.252948e-48 |
MsaG010328 | MsaG027627 | 0.806166 | 1.589861e-49 | 2.476988e-47 |
MsaG010372 | MsaG027627 | 0.836133 | 2.044121e-56 | 7.083793e-54 |
MsaG011039 | MsaG027627 | 0.801506 | 1.461534e-48 | 2.044456e-46 |
MsaG011219 | MsaG027627 | 0.807127 | 9.987244e-50 | 1.591709e-47 |
MsaG011248 | MsaG027627 | 0.825017 | 1.045437e-53 | 2.631108e-51 |
MsaG011286 | MsaG027627 | 0.817688 | 5.043807e-52 | 1.044277e-49 |
MsaG011405 | MsaG027627 | 0.811000 | 1.492600e-50 | 2.611860e-48 |
MsaG012216 | MsaG027627 | 0.810963 | 1.520261e-50 | 2.657891e-48 |
MsaG012203 | MsaG027627 | 0.844030 | 1.818029e-58 | 8.068671e-56 |
MsaG012398 | MsaG027627 | 0.817638 | 5.175367e-52 | 1.070142e-49 |
MsaG012553 | MsaG027627 | 0.814025 | 3.279648e-51 | 6.186219e-49 |
MsaG042079 | MsaG027627 | 0.840623 | 1.439121e-57 | 5.728814e-55 |
MsaG042142 | MsaG027627 | 0.819702 | 1.768850e-52 | 3.859623e-50 |
MsaG042674 | MsaG027627 | 0.818083 | 4.111842e-52 | 8.600577e-50 |
MsaG043392 | MsaG027627 | 0.827764 | 2.334105e-54 | 6.340814e-52 |
MsaG043500 | MsaG027627 | 0.803310 | 6.236805e-49 | 9.092118e-47 |
MsaG043815 | MsaG027627 | 0.830344 | 5.570681e-55 | 1.628108e-52 |
MsaG044048 | MsaG027627 | 0.804647 | 3.297856e-49 | 4.958917e-47 |
MsaG044233 | MsaG027627 | 0.820873 | 9.566371e-53 | 2.153082e-50 |
MsaG044623 | MsaG027627 | 0.863243 | 5.678794e-64 | 5.003741e-61 |
MsaG044880 | MsaG027627 | 0.836633 | 1.527734e-56 | 5.374280e-54 |
MsaG045245 | MsaG027627 | 0.805144 | 2.599377e-49 | 3.954098e-47 |
MsaG045536 | MsaG027627 | 0.831046 | 3.755435e-55 | 1.119910e-52 |
MsaG045873 | MsaG027627 | 0.830682 | 4.607640e-55 | 1.359689e-52 |
MsaG046926 | MsaG027627 | 0.802430 | 9.460426e-49 | 1.351545e-46 |
MsaG047073 | MsaG027627 | 0.820201 | 1.362301e-52 | 3.012306e-50 |
MsaG000837 | MsaG027627 | 0.843466 | 2.570339e-58 | 1.120332e-55 |
MsaG002575 | MsaG027627 | 0.808678 | 4.690418e-50 | 7.758815e-48 |
MsaG002237 | MsaG027627 | 0.810566 | 1.851242e-50 | 3.205316e-48 |
MsaG002745 | MsaG027627 | 0.839050 | 3.681650e-57 | 1.395179e-54 |
MsaG002774 | MsaG027627 | 0.820938 | 9.243308e-53 | 2.084049e-50 |
MsaG001894 | MsaG027627 | 0.815118 | 1.884713e-51 | 3.654012e-49 |
MsaG000843 | MsaG027627 | 0.827928 | 2.132398e-54 | 5.819666e-52 |
MsaG007370 | MsaG027627 | 0.811201 | 1.351069e-50 | 2.375854e-48 |
MsaG014863 | MsaG027627 | 0.813397 | 4.502942e-51 | 8.361093e-49 |
MsaG016146 | MsaG027627 | 0.856285 | 6.924534e-62 | 4.687585e-59 |
MsaG011633 | MsaG027627 | 0.810867 | 1.594783e-50 | 2.781565e-48 |
MsaG013857 | MsaG027627 | 0.802094 | 1.108317e-48 | 1.571226e-46 |
MsaG013372 | MsaG027627 | 0.817575 | 5.347739e-52 | 1.104016e-49 |
MsaG015300 | MsaG027627 | 0.812603 | 6.709502e-51 | 1.221368e-48 |
MsaG011352 | MsaG027627 | 0.836280 | 1.876643e-56 | 6.532309e-54 |
MsaG023458 | MsaG027627 | 0.807502 | 8.325779e-50 | 1.338798e-47 |
MsaG027627 | MsaG029395 | 0.801786 | 1.281434e-48 | 1.803948e-46 |
MsaG027627 | MsaG028127 | 0.822375 | 4.316825e-53 | 1.011242e-50 |
MsaG027627 | MsaG027793 | 0.807668 | 7.680284e-50 | 1.239908e-47 |
MsaG027627 | MsaG032120 | 0.802946 | 7.411759e-49 | 1.071450e-46 |
MsaG027627 | MsaG032342 | 0.822301 | 4.489347e-53 | 1.049577e-50 |
MsaG027627 | MsaG033140 | 0.814582 | 2.473678e-51 | 4.731550e-49 |
MsaG027627 | MsaG030136 | 0.809119 | 3.778941e-50 | 6.317591e-48 |
MsaG027627 | MsaG030752 | 0.831169 | 3.504659e-55 | 1.048801e-52 |
MsaG027627 | MsaG030000 | 0.807471 | 8.453029e-50 | 1.358234e-47 |
MsaG027627 | MsaG036358 | 0.809006 | 3.993279e-50 | 6.657925e-48 |
MsaG027627 | MsaG036808 | 0.804948 | 2.856437e-49 | 4.325247e-47 |
MsaG027627 | MsaG035293 | 0.844884 | 1.074429e-58 | 4.905125e-56 |
MsaG027627 | MsaG038711 | 0.805020 | 2.758490e-49 | 4.184136e-47 |
MsaG027627 | MsaG038618 | 0.801125 | 1.747341e-48 | 2.423263e-46 |
MsaG027627 | MsaG035918 | 0.808794 | 4.430807e-50 | 7.349686e-48 |
MsaG027627 | MsaG037442 | 0.825102 | 9.985181e-54 | 2.518837e-51 |
MsaG027627 | MsaG042416 | 0.804526 | 3.494856e-49 | 5.240433e-47 |
MsaG012778 | MsaG027627 | 0.826054 | 5.955115e-54 | 1.542384e-51 |
MsaG013321 | MsaG027627 | 0.812547 | 6.900377e-51 | 1.254400e-48 |
MsaG013912 | MsaG027627 | 0.803288 | 6.301561e-49 | 9.181962e-47 |
MsaG014215 | MsaG027627 | 0.818682 | 3.012739e-52 | 6.400095e-50 |
MsaG014483 | MsaG027627 | 0.820228 | 1.342903e-52 | 2.971578e-50 |
MsaG015143 | MsaG027627 | 0.800284 | 2.589793e-48 | 3.524004e-46 |
MsaG015689 | MsaG027627 | 0.843381 | 2.706422e-58 | 1.176455e-55 |
MsaG016078 | MsaG027627 | 0.805226 | 2.499396e-49 | 3.809178e-47 |
MsaG016163 | MsaG027627 | 0.805042 | 2.730670e-49 | 4.143979e-47 |
MsaG016250 | MsaG027627 | 0.808176 | 5.995325e-50 | 9.798023e-48 |
MsaG016646 | MsaG027627 | 0.803157 | 6.707228e-49 | 9.743387e-47 |
MsaG016994 | MsaG027627 | 0.810606 | 1.815292e-50 | 3.146075e-48 |
MsaG017198 | MsaG027627 | 0.805422 | 2.275516e-49 | 3.483694e-47 |
MsaG017552 | MsaG027627 | 0.808385 | 5.412103e-50 | 8.889238e-48 |
MsaG017589 | MsaG027627 | 0.807225 | 9.522773e-50 | 1.521157e-47 |
MsaG017614 | MsaG027627 | 0.832670 | 1.498864e-55 | 4.685891e-53 |
MsaG017728 | MsaG027627 | 0.805393 | 2.306445e-49 | 3.528779e-47 |
MsaG017734 | MsaG027627 | 0.811009 | 1.485953e-50 | 2.600814e-48 |
MsaG017888 | MsaG027627 | 0.819226 | 2.269016e-52 | 4.889608e-50 |
MsaG018113 | MsaG027627 | 0.820216 | 1.351381e-52 | 2.989354e-50 |
MsaG018168 | MsaG027627 | 0.815198 | 1.809068e-51 | 3.514690e-49 |
MsaG018309 | MsaG027627 | 0.805055 | 2.713354e-49 | 4.118901e-47 |
MsaG018808 | MsaG027627 | 0.831989 | 2.205700e-55 | 6.760992e-53 |
MsaG018876 | MsaG027627 | 0.811686 | 1.061068e-50 | 1.888251e-48 |
MsaG019132 | MsaG027627 | 0.844236 | 1.602037e-58 | 7.158876e-56 |
MsaG019273 | MsaG027627 | 0.819969 | 1.537993e-52 | 3.379905e-50 |
MsaG019542 | MsaG027627 | 0.806983 | 1.071200e-49 | 1.701400e-47 |
MsaG019676 | MsaG027627 | 0.813141 | 5.121361e-51 | 9.448554e-49 |
MsaG020391 | MsaG027627 | 0.809299 | 3.457775e-50 | 5.805560e-48 |
MsaG020525 | MsaG027627 | 0.824176 | 1.646244e-53 | 4.048916e-51 |
MsaG020618 | MsaG027627 | 0.822402 | 4.253939e-53 | 9.972408e-51 |
MsaG021062 | MsaG027627 | 0.843293 | 2.856308e-58 | 1.238117e-55 |
MsaG021423 | MsaG027627 | 0.826382 | 4.978973e-54 | 1.301355e-51 |
MsaG021463 | MsaG027627 | 0.829011 | 1.171052e-54 | 3.294487e-52 |
MsaG022052 | MsaG027627 | 0.839213 | 3.340901e-57 | 1.272394e-54 |
MsaG022056 | MsaG027627 | 0.852261 | 9.933271e-61 | 5.823362e-58 |
MsaG022227 | MsaG027627 | 0.810812 | 1.638350e-50 | 2.853817e-48 |
MsaG023205 | MsaG027627 | 0.816740 | 8.221814e-52 | 1.661269e-49 |
MsaG023304 | MsaG027627 | 0.801970 | 1.175149e-48 | 1.661278e-46 |
MsaG023415 | MsaG027627 | 0.805631 | 2.057516e-49 | 3.165461e-47 |
MsaG023475 | MsaG027627 | 0.823932 | 1.877098e-53 | 4.586261e-51 |
MsaG023680 | MsaG027627 | 0.810687 | 1.743239e-50 | 3.027176e-48 |
MsaG024286 | MsaG027627 | 0.801237 | 1.658680e-48 | 2.306041e-46 |
MsaG025002 | MsaG027627 | 0.803281 | 6.324371e-49 | 9.213584e-47 |
MsaG026579 | MsaG027627 | 0.804473 | 3.585271e-49 | 5.369352e-47 |
MsaG026975 | MsaG027627 | 0.804725 | 3.177188e-49 | 4.786154e-47 |
MsaG027159 | MsaG027627 | 0.811359 | 1.248732e-50 | 2.204453e-48 |
MsaG027383 | MsaG027627 | 0.804405 | 3.703473e-49 | 5.537707e-47 |
MsaG027650 | MsaG027627 | 0.814645 | 2.396467e-51 | 4.591139e-49 |
MsaG027743 | MsaG027627 | 0.839798 | 2.358055e-57 | 9.147190e-55 |
MsaG028043 | MsaG027627 | 0.803981 | 4.533854e-49 | 6.712980e-47 |
MsaG028256 | MsaG027627 | 0.846270 | 4.541579e-59 | 2.170340e-56 |
MsaG028286 | MsaG027627 | 0.816965 | 7.323449e-52 | 1.488358e-49 |
MsaG028350 | MsaG027627 | 0.800984 | 1.867359e-48 | 2.581437e-46 |
MsaG028633 | MsaG027627 | 0.811060 | 1.448761e-50 | 2.538952e-48 |
MsaG028634 | MsaG027627 | 0.803790 | 4.963958e-49 | 7.317408e-47 |
MsaG028635 | MsaG027627 | 0.817335 | 6.050953e-52 | 1.241535e-49 |
MsaG028886 | MsaG027627 | 0.803992 | 4.508684e-49 | 6.677603e-47 |
MsaG028935 | MsaG027627 | 0.816216 | 1.075579e-51 | 2.144325e-49 |
MsaG029004 | MsaG027627 | 0.837233 | 1.075158e-56 | 3.851544e-54 |
MsaG029075 | MsaG027627 | 0.812556 | 6.869529e-51 | 1.249061e-48 |
MsaG029309 | MsaG027627 | 0.803991 | 4.511833e-49 | 6.681969e-47 |
MsaG029470 | MsaG027627 | 0.811180 | 1.364864e-50 | 2.398916e-48 |
MsaG030237 | MsaG027627 | 0.812375 | 7.522801e-51 | 1.361668e-48 |
MsaG032228 | MsaG027627 | 0.813721 | 3.823968e-51 | 7.158174e-49 |
MsaG033863 | MsaG027627 | 0.801054 | 1.806906e-48 | 2.501756e-46 |
MsaG033975 | MsaG027627 | 0.812916 | 5.733899e-51 | 1.051947e-48 |
MsaG034418 | MsaG027627 | 0.806834 | 1.151034e-49 | 1.821763e-47 |
MsaG034667 | MsaG027627 | 0.846702 | 3.466982e-59 | 1.680789e-56 |
MsaG034673 | MsaG027627 | 0.821969 | 5.354782e-53 | 1.240793e-50 |
MsaG035190 | MsaG027627 | 0.803456 | 5.820155e-49 | 8.513272e-47 |
MsaG036509 | MsaG027627 | 0.821532 | 6.753265e-53 | 1.546727e-50 |
MsaG039429 | MsaG027627 | 0.827471 | 2.741947e-54 | 7.387648e-52 |
MsaG040350 | MsaG027627 | 0.814122 | 3.122529e-51 | 5.904281e-49 |
MsaG040381 | MsaG027627 | 0.866750 | 4.559972e-65 | 4.622678e-62 |
MsaG040474 | MsaG027627 | 0.858792 | 1.264121e-62 | 9.388336e-60 |
MsaG041224 | MsaG027627 | 0.800372 | 2.485751e-48 | 3.389199e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 11 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TACCGAACTCAAATTGATAA+AGG | 0.388384 | 5:-49088725 | MsaT027627.1:intron |
TACATCAGATTCTCGGCAAC+AGG | 0.398712 | 5:+49088562 | None:intergenic |
TACCTTTATCAATTTGAGTT+CGG | 0.422062 | 5:+49088723 | None:intergenic |
GAGATCAAGGGCAAAGAAAC+AGG | 0.423260 | 5:-49088903 | MsaT027627.1:intron |
GCGAATCTGCTGCGAGATCA+AGG | 0.428490 | 5:-49088916 | MsaT027627.1:CDS |
CACTACCTACATCAGATTCT+CGG | 0.442070 | 5:+49088555 | None:intergenic |
TTGATCTCGCAGCAGATTCG+CGG | 0.487789 | 5:+49088918 | None:intergenic |
ACAGATGGACAAATTTCAGT+TGG | 0.521534 | 5:-49088758 | MsaT027627.1:CDS |
ACACATAAATAAGTTACAGA+TGG | 0.576179 | 5:-49088773 | MsaT027627.1:CDS |
CGAATCTGCTGCGAGATCAA+GGG | 0.577446 | 5:-49088915 | MsaT027627.1:CDS |
TGTTGCCGAGAATCTGATGT+AGG | 0.606616 | 5:-49088560 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr5 | gene | 49088561 | 49088951 | 49088561 | ID=MsaG027627 |
Chr5 | mRNA | 49088561 | 49088951 | 49088561 | ID=MsaT027627.1;Parent=MsaG027627 |
Chr5 | exon | 49088561 | 49088590 | 49088561 | ID=MsaT027627.1.exon3;Parent=MsaT027627.1 |
Chr5 | CDS | 49088561 | 49088590 | 49088561 | ID=cds.MsaT027627.1;Parent=MsaT027627.1 |
Chr5 | exon | 49088726 | 49088797 | 49088726 | ID=MsaT027627.1.exon2;Parent=MsaT027627.1 |
Chr5 | CDS | 49088726 | 49088797 | 49088726 | ID=cds.MsaT027627.1;Parent=MsaT027627.1 |
Chr5 | exon | 49088904 | 49088951 | 49088904 | ID=MsaT027627.1.exon1;Parent=MsaT027627.1 |
Chr5 | CDS | 49088904 | 49088951 | 49088904 | ID=cds.MsaT027627.1;Parent=MsaT027627.1 |
Gene Sequence |
Protein sequence |