AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027629

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027629 MtrunA17_Chr5g0424861 96.657 359 11 1 16 373 1 359 0.0 721
MsaG027629 MtrunA17_Chr3g0115371 65.429 350 113 4 16 361 1 346 1.73e-158 449
MsaG027629 MtrunA17_Chr5g0424851 81.034 232 43 1 141 371 1 232 5.15e-132 385
MsaG027629 MtrunA17_Chr5g0424851 75.355 211 52 0 146 356 220 430 1.51e-109 327
MsaG027629 MtrunA17_Chr3g0124601 49.862 363 171 3 4 361 2 358 5.23e-118 346
MsaG027629 MtrunA17_Chr8g0382861 47.414 348 176 4 14 361 13 353 3.57e-100 301
MsaG027629 MtrunA17_Chr8g0353501 51.916 287 127 2 82 361 181 463 1.98e-90 279
MsaG027629 MtrunA17_Chr7g0256211 51.220 287 129 3 82 361 167 449 1.29e-89 277
MsaG027629 MtrunA17_Chr2g0321431 51.786 280 128 2 82 361 194 466 1.32e-89 278
MsaG027629 MtrunA17_Chr7g0258321 47.351 302 148 3 69 361 161 460 9.43e-89 275
MsaG027629 MtrunA17_Chr1g0174701 42.298 383 195 7 4 361 122 503 3.89e-86 270
MsaG027629 MtrunA17_Chr4g0026401 46.302 311 154 5 52 361 160 458 2.95e-84 264
MsaG027629 MtrunA17_Chr5g0422771 44.966 298 153 2 71 361 168 461 7.95e-79 249
MsaG027629 MtrunA17_Chr1g0210151 46.713 289 148 3 77 360 49 336 4.64e-78 243
MsaG027629 MtrunA17_Chr3g0093451 44.444 162 83 3 84 238 48 209 3.14e-41 144
MsaG027629 MtrunA17_Chr1g0196951 31.000 200 115 5 163 357 59 240 2.22e-15 75.1
MsaG027629 MtrunA17_Chr7g0242651 25.698 179 117 3 188 354 45 219 2.35e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027629 AT1G77920.1 53.050 377 147 6 1 361 1 363 1.11e-123 361
MsaG027629 AT1G22070.1 50.806 372 158 4 14 365 17 383 1.71e-118 348
MsaG027629 AT5G10030.5 50.693 361 172 3 4 361 2 359 1.20e-116 343
MsaG027629 AT5G10030.3 50.693 361 172 3 4 361 2 359 1.20e-116 343
MsaG027629 AT5G10030.1 50.693 361 172 3 4 361 2 359 1.20e-116 343
MsaG027629 AT5G10030.4 50.693 361 172 3 4 361 2 359 1.20e-116 343
MsaG027629 AT5G10030.2 50.693 361 172 3 4 361 2 359 1.20e-116 343
MsaG027629 AT5G65210.5 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.7 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.3 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.1 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.2 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.4 49.189 370 181 4 4 369 2 368 6.27e-116 342
MsaG027629 AT5G65210.6 53.846 299 134 2 72 369 3 298 1.07e-105 313
MsaG027629 AT1G08320.2 47.401 327 153 6 46 361 8 326 4.08e-92 280
MsaG027629 AT1G08320.5 47.401 327 153 6 46 361 8 326 4.08e-92 280
MsaG027629 AT1G08320.1 47.401 327 153 6 46 361 132 450 1.29e-90 281
MsaG027629 AT1G08320.4 47.401 327 153 6 46 361 132 450 1.29e-90 281
MsaG027629 AT1G08320.3 47.401 327 153 6 46 361 132 450 1.29e-90 281
MsaG027629 AT5G06839.1 45.614 342 165 5 37 361 75 412 1.12e-86 268
MsaG027629 AT5G06839.3 47.541 305 145 2 72 361 151 455 2.45e-86 269
MsaG027629 AT5G06950.2 48.951 286 136 2 82 361 46 327 5.68e-86 264
MsaG027629 AT5G06950.4 48.951 286 136 2 82 361 46 327 5.68e-86 264
MsaG027629 AT5G06950.1 48.951 286 136 2 82 361 46 327 5.68e-86 264
MsaG027629 AT5G06950.5 48.951 286 136 2 82 361 46 327 5.68e-86 264
MsaG027629 AT5G06950.3 48.951 286 136 2 82 361 46 327 5.68e-86 264
MsaG027629 AT5G06839.2 44.898 343 167 5 37 361 75 413 1.49e-85 266
MsaG027629 AT3G12250.5 48.951 286 136 2 82 361 19 300 3.52e-85 261
MsaG027629 AT3G12250.6 48.951 286 136 2 82 361 46 327 6.70e-85 261
MsaG027629 AT3G12250.2 48.951 286 136 2 82 361 46 327 6.70e-85 261
MsaG027629 AT3G12250.7 48.951 286 136 2 82 361 46 327 6.70e-85 261
MsaG027629 AT3G12250.1 48.951 286 136 2 82 361 46 327 6.70e-85 261
MsaG027629 AT3G12250.3 48.951 286 136 2 82 361 40 321 8.20e-85 261
MsaG027629 AT3G12250.4 48.951 286 136 2 82 361 71 352 3.36e-84 260
MsaG027629 AT5G06960.2 48.601 286 137 2 82 361 46 327 7.90e-84 258
MsaG027629 AT5G06960.1 48.601 286 137 2 82 361 46 327 7.90e-84 258
MsaG027629 AT5G06960.3 48.601 286 137 2 82 361 26 307 1.07e-83 257
MsaG027629 AT1G68640.1 44.966 298 152 3 72 361 156 449 2.88e-73 235
MsaG027629 AT1G58330.1 29.381 194 114 6 178 354 36 223 2.02e-12 66.6

Find 65 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGGATGTCGGTTATA+TGG 0.184139 5:+49158266 MsaT027629.1:CDS
TCTTTGTTTGAAATTGAATA+TGG 0.229069 5:+49158396 MsaT027629.1:CDS
CAAGCTGATCCCTTGGATTT+CGG 0.267505 5:+49159221 MsaT027629.1:CDS
TTTATACAGAAACAGTTTAT+TGG 0.277561 5:+49158251 MsaT027629.1:CDS
TGCTCAAGGCTTGTTGGCTA+TGG 0.316587 5:+49159553 MsaT027629.1:CDS
ATCCTCCTATCCAAAGAAAA+AGG 0.354680 5:-49158617 None:intergenic
TTATGTTCATCTTCAACATT+GGG 0.356119 5:-49157343 None:intergenic
TCATTCAAATTCTCAAGTTT+CGG 0.363992 5:-49159092 None:intergenic
GCTGAAGATGCACTGTCAAT+AGG 0.369583 5:+49159161 MsaT027629.1:CDS
CTTATGTTCATCTTCAACAT+TGG 0.379233 5:-49157344 None:intergenic
GCTCAAGGCTTGTTGGCTAT+GGG 0.379556 5:+49159554 MsaT027629.1:CDS
CTAACCTGAGTTTATTGTTC+CGG 0.383626 5:-49158295 None:intergenic
CTCAACTAATCGCGCCGAAA+AGG 0.387136 5:+49157234 MsaT027629.1:CDS
TCAAGCTGCTCAAGGCTTGT+TGG 0.395796 5:+49159547 MsaT027629.1:CDS
ACAGGCTTATGTTCAACAAC+TGG 0.406023 5:+49158066 MsaT027629.1:intron
CTTTCAGATGGCTGCTGCCA+TGG 0.412683 5:+49159253 MsaT027629.1:CDS
TATCGAGAAAACAAGAAAAC+AGG 0.417815 5:+49158126 MsaT027629.1:CDS
CTTTGCAGGCAGATCACCTT+AGG 0.420677 5:+49159483 MsaT027629.1:intron
TTATTGGATGTCGGTTATAT+GGG 0.423086 5:+49158267 MsaT027629.1:CDS
CCTCCCACATAGTCACTTGA+AGG 0.428980 5:-49157277 None:intergenic
AAGGTTCTCTTGATTCTTGA+GGG 0.435161 5:-49157492 None:intergenic
CCCTTGGATTTCGGAAACTA+CGG 0.436857 5:+49159230 MsaT027629.1:CDS
CAAGAATCAAGAGAACCTTC+TGG 0.437311 5:+49157496 MsaT027629.1:CDS
TACTGCTTCTCCTTTGTCCA+TGG 0.443791 5:-49159270 None:intergenic
CTGAAGATGCACTGTCAATA+GGG 0.444666 5:+49159162 MsaT027629.1:CDS
GACAAAGGAGAAGCAGTAGA+AGG 0.456711 5:+49159275 MsaT027629.1:CDS
AGTGTTGAGTAGGGTAGAAA+TGG 0.460652 5:-49159634 None:intergenic
CAACATTCAAGCTGATCCCT+TGG 0.462368 5:+49159214 MsaT027629.1:CDS
TCTTAATTTGAGTGTTGAGT+AGG 0.465347 5:-49159644 None:intergenic
GTCGGTTATATGGGAACGTC+CGG 0.469360 5:+49158276 MsaT027629.1:CDS
GAAGGTTCTCTTGATTCTTG+AGG 0.474792 5:-49157493 None:intergenic
TGTTGTTGATCATACTCTGC+TGG 0.478454 5:-49159196 None:intergenic
TCTATTTAATCTCTGGCGTA+TGG 0.481044 5:+49158577 MsaT027629.1:CDS
ATGTCACGCATCTTATCAAT+TGG 0.485700 5:+49159524 MsaT027629.1:CDS
CTCAAGGCTTGTTGGCTATG+GGG 0.490238 5:+49159555 MsaT027629.1:CDS
CTTAATTTGAGTGTTGAGTA+GGG 0.496496 5:-49159643 None:intergenic
CATAAGTAGCATGGAGAACA+AGG 0.509417 5:+49157361 MsaT027629.1:CDS
CACATAGTCACTTGAAGGAA+TGG 0.522645 5:-49157272 None:intergenic
CATTCCTTCAAGTGACTATG+TGG 0.523116 5:+49157273 MsaT027629.1:CDS
TCAAGGCTTGTTGGCTATGG+GGG 0.523504 5:+49159556 MsaT027629.1:CDS
AGATGCACTGTCAATAGGGT+TGG 0.527361 5:+49159166 MsaT027629.1:CDS
ATAACAGAGTTTGTTGCCTA+AGG 0.529419 5:-49159499 None:intergenic
TTGAAATTGAATATGGACGT+TGG 0.536052 5:+49158403 MsaT027629.1:CDS
TCAATTGGTCAAGCTGCTCA+AGG 0.536456 5:+49159539 MsaT027629.1:CDS
TTCGCACTCTTAGTTCATTG+TGG 0.540430 5:+49159594 MsaT027629.1:CDS
TATTCAAGAACCTGTTCCTA+AGG 0.542554 5:+49157534 MsaT027629.1:CDS
TAGATTGAAGCTCATGCAGT+TGG 0.542577 5:+49158096 MsaT027629.1:CDS
CGGAAACTACGGCTTTCAGA+TGG 0.543160 5:+49159241 MsaT027629.1:CDS
AATGAAAGCAGAAGCTGCAA+AGG 0.554661 5:+49158545 MsaT027629.1:CDS
AGAAACAGTTTATTGGATGT+CGG 0.555658 5:+49158258 MsaT027629.1:CDS
GCCGTAGTTTCCGAAATCCA+AGG 0.586415 5:-49159231 None:intergenic
GAAGATGAACATAAGTAGCA+TGG 0.592197 5:+49157352 MsaT027629.1:CDS
TGTACATTTAGAATTTGTGA+TGG 0.598170 5:-49158645 None:intergenic
GCGCGGCTGCTCGAAAATGT+CGG 0.599361 5:+49157962 MsaT027629.1:CDS
ATGGCTGCTGCCATGGACAA+AGG 0.602885 5:+49159260 MsaT027629.1:CDS
GTCTCTTGATCATCTCCAGA+AGG 0.603267 5:-49157511 None:intergenic
CTCTGGCGTATGGAAATCAC+CGG 0.616184 5:+49158587 MsaT027629.1:CDS
TTCATCTACTTGTCGAGAGT+AGG 0.630773 5:+49158496 MsaT027629.1:CDS
CCGTAGTTTCCGAAATCCAA+GGG 0.636532 5:-49159230 None:intergenic
CCTTCAAGTGACTATGTGGG+AGG 0.650193 5:+49157277 MsaT027629.1:CDS
ATTCCTTCAAGTGACTATGT+GGG 0.664208 5:+49157274 MsaT027629.1:CDS
ACGTCCGGAACAATAAACTC+AGG 0.666698 5:+49158291 MsaT027629.1:CDS
CTTGCTGAGATGATAAACGC+AGG 0.683767 5:-49159139 None:intergenic
TATGTTCATCTTCAACATTG+GGG 0.698417 5:-49157342 None:intergenic
ACGTCAAGCACAGAATCGCG+CGG 0.719929 5:+49157945 MsaT027629.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 49157212 49159664 49157212 ID=MsaG027629
Chr5 mRNA 49157212 49159664 49157212 ID=MsaT027629.1;Parent=MsaG027629
Chr5 exon 49157212 49157382 49157212 ID=MsaT027629.1.exon1;Parent=MsaT027629.1
Chr5 CDS 49157212 49157382 49157212 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49157481 49157555 49157481 ID=MsaT027629.1.exon2;Parent=MsaT027629.1
Chr5 CDS 49157481 49157555 49157481 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49157937 49157996 49157937 ID=MsaT027629.1.exon3;Parent=MsaT027629.1
Chr5 CDS 49157937 49157996 49157937 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49158070 49158147 49158070 ID=MsaT027629.1.exon4;Parent=MsaT027629.1
Chr5 CDS 49158070 49158147 49158070 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49158249 49158312 49158249 ID=MsaT027629.1.exon5;Parent=MsaT027629.1
Chr5 CDS 49158249 49158312 49158249 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49158391 49158662 49158391 ID=MsaT027629.1.exon6;Parent=MsaT027629.1
Chr5 CDS 49158391 49158662 49158391 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49159083 49159310 49159083 ID=MsaT027629.1.exon7;Parent=MsaT027629.1
Chr5 CDS 49159083 49159310 49159083 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Chr5 exon 49159491 49159664 49159491 ID=MsaT027629.1.exon8;Parent=MsaT027629.1
Chr5 CDS 49159491 49159664 49159491 ID=cds.MsaT027629.1;Parent=MsaT027629.1
Gene Sequence

>MsaT027629.1

ATGAACTCTTCTTCATCATCAACTCAACTAATCGCGCCGAAAAGGATGAACATGTATGAGCCATTCCTTCAAGTGACTATGTGGGAGGATAGCTTTAAAGTTGATCATAGTACTTTGAATTCAATTGCTTCCCCAATGTTGAAGATGAACATAAGTAGCATGGAGAACAAGTCTGAATGTATCCCTCAAGAATCAAGAGAACCTTCTGGAGATGATCAAGAGACTATTCAAGAACCTGTTCCTAAGGTACTACGACGTCAAGCACAGAATCGCGCGGCTGCTCGAAAATGTCGGCTGCGAAAAAAGGCTTATGTTCAACAACTGGAAACGAGTAGATTGAAGCTCATGCAGTTGGAATTAGATATCGAGAAAACAAGAAAACAGGGTTTATACAGAAACAGTTTATTGGATGTCGGTTATATGGGAACGTCCGGAACAATAAACTCAGGGATATCTTTGTTTGAAATTGAATATGGACGTTGGATTGAAGAACAAGATCGACAAAACGAAGAACTAAGAAATGCATTGCAAACTAATGCATCTGATATACATCTTCATCTACTTGTCGAGAGTAGGTTGAACCAATACTCAAATCTTTTTCAAATGAAAGCAGAAGCTGCAAAGGCTGATGTTTTCTATTTAATCTCTGGCGTATGGAAATCACCGGTCGAACGCCTTTTTCTTTGGATAGGAGGATCTCGTCCATCACAAATTCTAAATATCGTGGTACCGAAACTTGAGAATTTGAATGATCAACAAATTGCTAGCATTAACAACCTGCGTTTATCATCTCAGCAAGCTGAAGATGCACTGTCAATAGGGTTGGAGAAACTCCAGCAGAGTATGATCAACAACATTCAAGCTGATCCCTTGGATTTCGGAAACTACGGCTTTCAGATGGCTGCTGCCATGGACAAAGGAGAAGCAGTAGAAGGTTTTGTAATTCAGGCAGATCACCTTAGGCAACAAACTCTGTTATACATGTCACGCATCTTATCAATTGGTCAAGCTGCTCAAGGCTTGTTGGCTATGGGGGAATACTTTCATCGTCTTCGCACTCTTAGTTCATTGTGGACTGCTCGTTCATGTCACCATTTCTACCCTACTCAACACTCAAATTAA
Protein sequence

>MsaT027629.1

MNSSSSSTQLIAPKRMNMYEPFLQVTMWEDSFKVDHSTLNSIASPMLKMNISSMENKSECIPQESREPSGDDQETIQEPVPKVLRRQAQNRAAARKCRLRKKAYVQQLETSRLKLMQLELDIEKTRKQGLYRNSLLDVGYMGTSGTINSGISLFEIEYGRWIEEQDRQNEELRNALQTNASDIHLHLLVESRLNQYSNLFQMKAEAAKADVFYLISGVWKSPVERLFLWIGGSRPSQILNIVVPKLENLNDQQIASINNLRLSSQQAEDALSIGLEKLQQSMINNIQADPLDFGNYGFQMAAAMDKGEAVEGFVIQADHLRQQTLLYMSRILSIGQAAQGLLAMGEYFHRLRTLSSLWTARSCHHFYPTQHSN*