AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027637


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027637 MtrunA17_Chr5g0425261 60.847 378 105 6 1 336 1 377 2.94e-143 410
MsaG027637 MtrunA17_Chr5g0424801 60.582 378 106 6 1 336 1 377 6.12e-142 407
MsaG027637 MtrunA17_Chr5g0424741 49.735 378 122 8 1 311 1 377 8.97e-105 317
MsaG027637 MtrunA17_Chr5g0424931 48.642 405 115 14 1 321 8 403 3.65e-103 313
MsaG027637 MtrunA17_Chr1g0178011 46.366 399 153 14 1 348 1 389 3.97e-92 283
MsaG027637 MtrunA17_Chr5g0424791 87.857 140 16 1 33 171 12 151 2.12e-77 234
MsaG027637 MtrunA17_Chr5g0417281 43.155 336 141 11 8 304 7 331 1.19e-71 231
MsaG027637 MtrunA17_Chr2g0289221 44.172 326 121 13 4 284 12 321 1.66e-69 221
MsaG027637 MtrunA17_Chr2g0277461 42.151 344 142 13 5 304 6 336 4.57e-67 218
MsaG027637 MtrunA17_Chr5g0417211 43.027 337 128 13 5 294 5 324 8.85e-67 216
MsaG027637 MtrunA17_Chr5g0436761 38.133 375 173 11 5 333 5 366 3.90e-66 216
MsaG027637 MtrunA17_Chr2g0277531 38.438 320 141 10 6 284 14 318 3.12e-57 188
MsaG027637 MtrunA17_Chr3g0115271 34.626 361 169 11 30 337 63 409 6.25e-48 170
MsaG027637 MtrunA17_Chr5g0425651 63.393 112 39 2 197 307 1 111 9.09e-42 142
MsaG027637 MtrunA17_Chr1g0181211 31.008 387 174 15 11 310 26 406 1.44e-36 139
MsaG027637 MtrunA17_Chr2g0326761 30.108 372 176 14 11 304 10 375 1.19e-35 137
MsaG027637 MtrunA17_Chr1g0199681 31.594 345 166 11 24 309 130 463 4.33e-35 135
MsaG027637 MtrunA17_Chr4g0028971 29.517 393 188 14 1 309 1 388 1.52e-33 131
MsaG027637 MtrunA17_Chr5g0425661 43.005 193 71 6 12 166 37 228 5.85e-32 120
MsaG027637 MtrunA17_Chr7g0264171 30.058 346 170 14 31 310 74 413 1.37e-30 122
MsaG027637 MtrunA17_Chr2g0276781 26.425 386 210 13 9 338 20 387 3.40e-27 112
MsaG027637 MtrunA17_Chr7g0239061 28.772 285 181 8 35 304 67 344 1.83e-25 107
MsaG027637 MtrunA17_Chr7g0264161 31.022 274 162 12 31 284 35 301 6.69e-25 102
MsaG027637 MtrunA17_Chr4g0009751 28.920 287 178 9 35 304 72 349 9.77e-25 105
MsaG027637 MtrunA17_Chr2g0282961 25.648 347 212 11 2 304 43 387 1.94e-24 104
MsaG027637 MtrunA17_Chr8g0385791 27.815 302 193 8 25 309 103 396 2.61e-24 104
MsaG027637 MtrunA17_Chr2g0285461 27.557 352 179 16 12 304 23 357 3.23e-24 103
MsaG027637 MtrunA17_Chr3g0109391 27.508 309 175 11 26 304 71 360 2.08e-22 98.6
MsaG027637 MtrunA17_Chr5g0433301 28.479 309 172 12 26 304 69 358 2.54e-22 98.6
MsaG027637 MtrunA17_Chr8g0376771 27.221 349 183 16 12 304 23 356 3.35e-22 98.2
MsaG027637 MtrunA17_Chr4g0070181 25.439 342 213 8 9 309 20 360 1.51e-21 96.3
MsaG027637 MtrunA17_Chr2g0301251 26.936 297 198 6 31 309 65 360 4.25e-21 94.7
MsaG027637 MtrunA17_Chr8g0346711 26.531 343 195 13 9 304 26 358 3.35e-20 92.0
MsaG027637 MtrunA17_Chr1g0156321 28.716 296 181 12 31 304 89 376 1.00e-19 90.5
MsaG027637 MtrunA17_Chr4g0046371 24.269 342 207 12 12 304 38 376 1.77e-17 84.0
MsaG027637 MtrunA17_Chr8g0389081 24.561 342 212 8 7 304 27 366 2.62e-17 83.6
MsaG027637 MtrunA17_Chr4g0029671 26.019 319 176 12 26 304 98 396 4.77e-17 82.4
MsaG027637 MtrunA17_Chr2g0326281 26.198 313 189 12 30 304 103 411 1.07e-15 78.6
MsaG027637 MtrunA17_Chr8g0356211 24.686 239 151 7 92 313 3 229 4.01e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027637 AT1G77850.2 31.063 367 179 14 8 304 15 377 9.40e-45 162
MsaG027637 AT1G77850.1 31.063 367 179 14 8 304 15 377 9.78e-45 162
MsaG027637 AT4G30080.1 31.367 373 170 15 11 304 18 383 3.36e-37 142
MsaG027637 AT2G28350.1 29.427 384 181 17 11 309 9 387 6.22e-34 132
MsaG027637 AT2G28350.2 29.427 384 181 17 11 309 9 387 6.44e-34 132
MsaG027637 AT2G46530.1 30.952 294 169 11 31 304 71 350 9.48e-29 117
MsaG027637 AT2G46530.3 30.952 294 169 11 31 304 92 371 1.16e-28 117
MsaG027637 AT3G61830.1 26.724 348 190 12 8 304 21 354 8.60e-27 111
MsaG027637 AT3G61830.2 29.592 294 173 11 31 304 24 303 8.62e-27 111
MsaG027637 AT1G35520.1 29.677 310 182 13 35 326 80 371 5.32e-26 109
MsaG027637 AT1G34390.1 26.966 356 185 13 9 309 24 359 8.57e-26 108
MsaG027637 AT1G59750.2 27.353 340 192 11 12 304 22 353 1.54e-25 108
MsaG027637 AT1G59750.4 27.353 340 192 11 12 304 22 353 1.66e-25 108
MsaG027637 AT1G59750.1 27.353 340 192 11 12 304 22 353 1.72e-25 108
MsaG027637 AT1G59750.3 27.353 340 192 11 12 304 22 353 1.72e-25 108
MsaG027637 AT4G23980.2 28.000 300 193 8 31 313 62 355 3.89e-25 107
MsaG027637 AT4G23980.1 28.000 300 193 8 31 313 62 355 4.13e-25 107
MsaG027637 AT1G34410.1 30.033 303 171 13 35 313 80 365 9.58e-25 105
MsaG027637 AT1G34310.1 27.933 358 189 15 9 313 24 365 2.21e-24 104
MsaG027637 AT1G35240.1 29.568 301 175 12 35 313 80 365 3.04e-24 104
MsaG027637 AT1G30330.1 26.703 367 186 15 2 304 10 357 3.34e-24 104
MsaG027637 AT1G30330.3 26.836 354 179 14 12 304 25 359 4.72e-24 103
MsaG027637 AT1G30330.2 26.836 354 179 14 12 304 25 359 4.72e-24 103
MsaG027637 AT2G33860.1 28.487 337 187 15 31 331 96 414 8.17e-24 103
MsaG027637 AT5G37020.2 26.271 354 181 15 12 304 22 356 9.58e-24 103
MsaG027637 AT5G37020.1 26.271 354 181 15 12 304 22 356 1.23e-23 102
MsaG027637 AT5G62000.4 26.821 302 177 9 35 309 114 398 3.07e-23 101
MsaG027637 AT5G62000.2 26.821 302 177 9 35 309 114 398 3.25e-23 101
MsaG027637 AT5G62000.5 26.821 302 177 9 35 309 114 398 3.25e-23 101
MsaG027637 AT5G62000.3 26.821 302 177 9 35 309 114 398 3.25e-23 101
MsaG027637 AT5G62000.1 26.821 302 177 9 35 309 114 398 3.25e-23 101
MsaG027637 AT2G46530.2 30.709 254 146 10 67 304 24 263 6.28e-23 100
MsaG027637 AT1G35540.1 26.822 343 190 14 9 304 24 352 7.40e-23 100
MsaG027637 AT2G33860.2 28.519 270 156 12 85 331 2 257 1.58e-20 92.8
MsaG027637 AT5G60450.1 25.000 352 200 13 9 304 62 405 1.82e-19 90.1
MsaG027637 AT5G60450.2 25.000 352 200 13 9 304 38 381 2.12e-19 90.1
MsaG027637 AT1G34170.1 27.666 347 184 15 9 304 22 352 2.31e-19 89.4
MsaG027637 AT1G19850.1 27.660 282 191 6 35 304 108 388 1.68e-18 87.4
MsaG027637 AT1G19850.2 27.660 282 191 6 35 304 97 377 1.73e-18 87.4
MsaG027637 AT5G20730.1 25.507 345 210 11 9 309 21 362 2.07e-18 87.4
MsaG027637 AT5G20730.3 25.507 345 210 11 9 309 21 362 2.22e-18 87.0
MsaG027637 AT5G20730.2 25.797 345 208 12 9 309 21 361 3.36e-18 86.7
MsaG027637 AT1G19220.1 25.874 286 200 5 35 309 76 360 1.13e-17 85.1
MsaG027637 AT5G37020.3 28.571 245 136 10 85 304 31 261 1.91e-17 84.0
MsaG027637 AT1G34170.2 27.089 347 160 17 9 304 22 326 3.19e-13 70.9
MsaG027637 AT1G34170.3 27.089 347 160 17 9 304 22 326 4.14e-13 70.9

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCCATCTTCGTCTTCT+TGG 0.210312 5:-49356356 None:intergenic
CCTGAAAACAACAGAATCTT+TGG 0.226595 5:-49356601 None:intergenic
ATTCTTCAAATGTCCCTAAT+TGG 0.230817 5:+49356952 MsaT027637.1:CDS
ATGAAGATTGGTTGGGAATC+TGG 0.311617 5:+49356837 MsaT027637.1:CDS
CGTAGGTGCAACCCCATTCT+TGG 0.311998 5:-49356518 None:intergenic
CTTTCACCCGCTTCAGCTGC+AGG 0.324014 5:-49356675 None:intergenic
ACCTCAAGAATGCGCCAATT+AGG 0.328543 5:-49356966 None:intergenic
GGCAACAAGTCAGCCGATTT+AGG 0.332336 5:-49357790 None:intergenic
TCCATCTTCGTCTTCTTGGC+CGG 0.332884 5:-49356352 None:intergenic
CTGCTCCTCACTCCGGTAAC+TGG 0.333359 5:+49356300 MsaT027637.1:CDS
GACTTCTCCAAGTCAAGCTT+TGG 0.342525 5:-49356465 None:intergenic
ATCTTTGGAACCTTGGCATC+GGG 0.343346 5:-49356105 None:intergenic
CCTCAAGAATGCGCCAATTA+GGG 0.362876 5:-49356965 None:intergenic
CCATCTTCGTCTTCTTGGCC+GGG 0.390410 5:-49356351 None:intergenic
GGAGGTTCTTGGCCAGTTAC+CGG 0.390862 5:-49356312 None:intergenic
GCCATGCCTCTGCCTGAGCC+AGG 0.396403 5:+49356045 None:intergenic
AGATTCCCAACCAATCTTCA+TGG 0.412679 5:-49356835 None:intergenic
GTACTGGCTGGTCTCACTTT+CGG 0.420748 5:+49356559 MsaT027637.1:CDS
CAGTAGGAGGAGGAGGTTCT+TGG 0.423692 5:-49356323 None:intergenic
CGACCTTAAAAGCCAAGAAT+GGG 0.432454 5:+49356506 MsaT027637.1:CDS
CCTCCTCCTACTGTTGTTCC+CGG 0.432625 5:+49356333 MsaT027637.1:CDS
GCCGTGTCCACCCTAAAATA+TGG 0.440684 5:+49356067 MsaT027637.1:CDS
GGGTGGACACGGCCTGGCTC+AGG 0.447654 5:-49356057 None:intergenic
ATACACTGGCAGTCCTAAAT+CGG 0.448630 5:+49357777 MsaT027637.1:CDS
ATTATCCGACAGCTAGGAAT+TGG 0.450128 5:+49356775 MsaT027637.1:CDS
TCTGCTGGAAAGATATTAGT+TGG 0.452740 5:+49356630 MsaT027637.1:CDS
CCGCACTCTCAGTTAAATTC+CGG 0.457010 5:-49356701 None:intergenic
GATCTTTCCAAAGCTTGACT+TGG 0.477680 5:+49356458 MsaT027637.1:CDS
TACCGGAGTGAGGAGCAGTT+TGG 0.479172 5:-49356295 None:intergenic
AACTTCCCTGCAGCTGAAGC+GGG 0.481484 5:+49356669 MsaT027637.1:CDS
ATCAGGAAAAGTAGAATGGC+AGG 0.482347 5:+49357724 MsaT027637.1:CDS
TTATCATAGGTTAACTGGGA+TGG 0.485250 5:+49357604 MsaT027637.1:intron
ACAGTAGAGATTGTTCCCTT+AGG 0.494524 5:-49356921 None:intergenic
ACACGGCCTGGCTCAGGCAG+AGG 0.496843 5:-49356051 None:intergenic
ACAAGGGTGATGGTTTCATC+AGG 0.497114 5:+49357707 MsaT027637.1:CDS
GTAGATGATGCCATGAAGAT+TGG 0.497274 5:+49356825 MsaT027637.1:CDS
CCCTAATTGGCGCATTCTTG+AGG 0.497342 5:+49356965 MsaT027637.1:CDS
GCAGCTGAAGCGGGTGAAAG+AGG 0.499828 5:+49356678 MsaT027637.1:CDS
AGCATCAACAAAGAGATTAA+CGG 0.500852 5:-49356253 None:intergenic
CCGACCTTAAAAGCCAAGAA+TGG 0.501034 5:+49356505 MsaT027637.1:CDS
AAAGACATATTATAAGCCTA+AGG 0.505882 5:+49356905 MsaT027637.1:CDS
ATACAAACATAGTCAATCCT+TGG 0.507379 5:+49357644 MsaT027637.1:CDS
AGATATTAGTTGGAATTCGC+AGG 0.508600 5:+49356640 MsaT027637.1:CDS
TTTGAATACAGTTGAATTAG+CGG 0.512559 5:+49356725 MsaT027637.1:CDS
ACTTGTTGCTTGGTTCATTG+AGG 0.515871 5:-49357811 None:intergenic
CTGAAGCGGGTGAAAGAGGC+CGG 0.516670 5:+49356682 MsaT027637.1:CDS
AATCTTTGGAACCTTGGCAT+CGG 0.533073 5:-49356106 None:intergenic
CGCCAAACTGCTCCTCACTC+CGG 0.538251 5:+49356293 MsaT027637.1:CDS
AAAATATGGCAGATATGTGC+AGG 0.539162 5:+49356081 MsaT027637.1:CDS
GCCTGGCTCAGGCAGAGGCA+TGG 0.543238 5:-49356046 None:intergenic
TAACTTCCCTGCAGCTGAAG+CGG 0.546068 5:+49356668 MsaT027637.1:CDS
TTTCATCAGGAAAAGTAGAA+TGG 0.549729 5:+49357720 MsaT027637.1:CDS
ATGATGCCATGAAGATTGGT+TGG 0.554453 5:+49356829 MsaT027637.1:CDS
ATGTGCAGGCCCCGATGCCA+AGG 0.559547 5:+49356095 MsaT027637.1:CDS
TTGCAAGCTTCAAGCTGCCA+AGG 0.562587 5:-49357661 None:intergenic
TTGTGTATTATCCGACAGCT+AGG 0.565398 5:+49356769 MsaT027637.1:CDS
CCCGGCCAAGAAGACGAAGA+TGG 0.565421 5:+49356351 MsaT027637.1:CDS
GAAATGATGCATGGAAAGGA+TGG 0.568677 5:-49356228 None:intergenic
TGATGCCATGAAGATTGGTT+GGG 0.569149 5:+49356830 MsaT027637.1:CDS
TTTCACCCGCTTCAGCTGCA+GGG 0.570879 5:-49356674 None:intergenic
GGAACAACAGTAGGAGGAGG+AGG 0.574344 5:-49356330 None:intergenic
TCACACCAATTCCTAGCTGT+CGG 0.585707 5:-49356780 None:intergenic
TCTTGGCCGGGAACAACAGT+AGG 0.588215 5:-49356339 None:intergenic
CTTGGCCAGTTACCGGAGTG+AGG 0.591769 5:-49356305 None:intergenic
TGAATTAGCGGACAAGAACA+TGG 0.593074 5:+49356737 MsaT027637.1:CDS
AAGACATATTATAAGCCTAA+GGG 0.594017 5:+49356906 MsaT027637.1:CDS
TGTTGATGCTCTCACTGACG+AGG 0.594122 5:+49356266 MsaT027637.1:CDS
TTAACGGCTGAAATGATGCA+TGG 0.595581 5:-49356237 None:intergenic
CCGGGAACAACAGTAGGAGG+AGG 0.601910 5:-49356333 None:intergenic
ACAGTAGACTACGTACTGGC+TGG 0.603214 5:+49356547 MsaT027637.1:CDS
CCGGAATTTAACTGAGAGTG+CGG 0.606695 5:+49356701 MsaT027637.1:CDS
TGGCCGGGAACAACAGTAGG+AGG 0.609021 5:-49356336 None:intergenic
AGTAGAATGGCAGGGCTGAG+TGG 0.615590 5:+49357733 MsaT027637.1:CDS
ATCAAGTCAGCGAATTCATG+AGG 0.631551 5:-49356438 None:intergenic
GGCTGAAATGATGCATGGAA+AGG 0.633559 5:-49356232 None:intergenic
TCTTTGGAACCTTGGCATCG+GGG 0.654059 5:-49356104 None:intergenic
TGATGCATGGAAAGGATGGA+CGG 0.656958 5:-49356224 None:intergenic
TCAGGAAAAGTAGAATGGCA+GGG 0.663509 5:+49357725 MsaT027637.1:CDS
TCTGACACAGAGCTATACAC+TGG 0.679314 5:+49357763 MsaT027637.1:CDS
GACCTTAAAAGCCAAGAATG+GGG 0.680749 5:+49356507 MsaT027637.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 49356048 49357819 49356048 ID=MsaG027637
Chr5 mRNA 49356048 49357819 49356048 ID=MsaT027637.1;Parent=MsaG027637
Chr5 exon 49356048 49356116 49356048 ID=MsaT027637.1.exon1;Parent=MsaT027637.1
Chr5 CDS 49356048 49356116 49356048 ID=cds.MsaT027637.1;Parent=MsaT027637.1
Chr5 exon 49356207 49356986 49356207 ID=MsaT027637.1.exon2;Parent=MsaT027637.1
Chr5 CDS 49356207 49356986 49356207 ID=cds.MsaT027637.1;Parent=MsaT027637.1
Chr5 exon 49357613 49357819 49357613 ID=MsaT027637.1.exon3;Parent=MsaT027637.1
Chr5 CDS 49357613 49357819 49357613 ID=cds.MsaT027637.1;Parent=MsaT027637.1
Gene Sequence

>MsaT027637.1

ATGCCTCTGCCTGAGCCAGGCCGTGTCCACCCTAAAATATGGCAGATATGTGCAGGCCCCGATGCCAAGTTTCTTTATGATCGTTACCGTCCATCCTTTCCATGCATCATTTCAGCCGTTAATCTCTTTGTTGATGCTCTCACTGACGAGGTTTTCGCCAAACTGCTCCTCACTCCGGTAACTGGCCAAGAACCTCCTCCTCCTACTGTTGTTCCCGGCCAAGAAGACGAAGATGGAAACCATCTTGTTTCCTATGTCAAAACTCTCACAACAACTGATACTAAATCTGTATTCAAGATACCTCATGAATTCGCTGACTTGATCTTTCCAAAGCTTGACTTGGAGAAGTCTCAAAACATCACTGTCACCGACCTTAAAAGCCAAGAATGGGGTTGCACCTACGTGAAAAACAGTAGACTACGTACTGGCTGGTCTCACTTTCGGAAAGAGAAGAAACTCGTAGCCAAAGATTCTGTTGTTTTCAGGAAAAACTCTGCTGGAAAGATATTAGTTGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAGCGGGTGAAAGAGGCCGGAATTTAACTGAGAGTGCGGTTTTGAATACAGTTGAATTAGCGGACAAGAACATGGAGTTTGAAGTTGTGTATTATCCGACAGCTAGGAATTGGTGTGATTTTGTGATGGATGCTAAGGTTGTAGATGATGCCATGAAGATTGGTTGGGAATCTGGAATGAGAGTCAAACTTACGTTGAAGAAAGATGAATCTTCAAATTCAAAGACATATTATAAGCCTAAGGGAACAATCTCTACTGTCTTTAATTATTCTTCAAATGTCCCTAATTGGCGCATTCTTGAGGTTAACTGGGATGGACCTAAAATCTTGCAAAATACAAACATAGTCAATCCTTGGCAGCTTGAAGCTTGCAACATACCTGCAAAAGTTTTGAATGACAAGGGTGATGGTTTCATCAGGAAAAGTAGAATGGCAGGGCTGAGTGGTACTTTGTCTGACACAGAGCTATACACTGGCAGTCCTAAATCGGCTGACTTGTTGCCTCAATGA

Protein sequence

>MsaT027637.1

MPLPEPGRVHPKIWQICAGPDAKFLYDRYRPSFPCIISAVNLFVDALTDEVFAKLLLTPVTGQEPPPPTVVPGQEDEDGNHLVSYVKTLTTTDTKSVFKIPHEFADLIFPKLDLEKSQNITVTDLKSQEWGCTYVKNSRLRTGWSHFRKEKKLVAKDSVVFRKNSAGKILVGIRRNTNFPAAEAGERGRNLTESAVLNTVELADKNMEFEVVYYPTARNWCDFVMDAKVVDDAMKIGWESGMRVKLTLKKDESSNSKTYYKPKGTISTVFNYSSNVPNWRILEVNWDGPKILQNTNIVNPWQLEACNIPAKVLNDKGDGFIRKSRMAGLSGTLSDTELYTGSPKSADLLPQ*