AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027638 MtrunA17_Chr5g0425261 91.281 367 24 2 242 605 16 377 0.0 676
MsaG027638 MtrunA17_Chr5g0424801 89.918 367 29 2 242 605 16 377 0.0 664
MsaG027638 MtrunA17_Chr5g0424761 90.777 206 19 0 1 206 102 307 4.48e-130 385
MsaG027638 MtrunA17_Chr5g0424741 54.229 402 131 8 242 593 16 414 1.72e-120 367
MsaG027638 MtrunA17_Chr5g0424931 53.826 379 134 9 243 590 24 392 2.53e-119 364
MsaG027638 MtrunA17_Chr1g0178011 53.151 365 135 10 243 590 17 362 4.27e-108 333
MsaG027638 MtrunA17_Chr5g0417281 49.240 329 144 9 243 561 16 331 9.01e-88 282
MsaG027638 MtrunA17_Chr2g0289221 51.420 317 128 12 243 547 25 327 1.61e-84 268
MsaG027638 MtrunA17_Chr5g0424701 81.529 157 29 0 1 157 101 257 8.28e-83 260
MsaG027638 MtrunA17_Chr5g0417211 51.274 314 122 11 243 541 17 314 1.47e-79 257
MsaG027638 MtrunA17_Chr2g0277461 43.850 374 177 13 243 600 18 374 3.49e-79 257
MsaG027638 MtrunA17_Chr5g0436761 45.509 334 153 10 244 562 18 337 7.89e-78 254
MsaG027638 MtrunA17_Chr3g0085941 60.476 210 79 1 1 206 99 308 6.32e-70 230
MsaG027638 MtrunA17_Chr2g0277531 43.408 311 147 12 243 541 25 318 1.56e-65 217
MsaG027638 MtrunA17_Chr5g0425661 57.592 191 65 7 243 421 42 228 1.05e-59 199
MsaG027638 MtrunA17_Chr3g0115271 33.820 411 194 11 244 604 25 407 4.76e-56 199
MsaG027638 MtrunA17_Chr5g0427711 50.943 212 97 4 1 206 87 297 2.34e-55 191
MsaG027638 MtrunA17_Chr7g0249591 52.133 211 94 4 1 206 98 306 4.77e-55 192
MsaG027638 MtrunA17_Chr1g0199681 35.149 404 182 16 243 587 99 481 1.64e-54 197
MsaG027638 MtrunA17_Chr1g0181211 36.979 384 174 13 243 567 32 406 1.04e-53 194
MsaG027638 MtrunA17_Chr3g0091201 50.237 211 99 3 1 206 113 322 8.98e-52 181
MsaG027638 MtrunA17_Chr7g0221741 49.528 212 101 3 1 206 92 303 1.06e-51 181
MsaG027638 MtrunA17_Chr2g0326761 35.580 371 178 12 243 562 16 376 3.68e-50 184
MsaG027638 MtrunA17_Chr4g0028971 35.602 382 178 12 243 566 17 388 8.86e-50 184
MsaG027638 MtrunA17_Chr6g0457681 47.291 203 98 3 6 206 159 354 1.21e-49 177
MsaG027638 MtrunA17_Chr5g0424791 66.197 142 44 2 287 426 12 151 3.32e-48 165
MsaG027638 MtrunA17_Chr7g0221701 50.239 209 97 5 4 207 68 274 3.43e-48 171
MsaG027638 MtrunA17_Chr6g0457641 46.535 202 105 3 6 206 141 340 7.50e-48 171
MsaG027638 MtrunA17_Chr4g0049031 50.952 210 95 4 1 206 112 317 9.59e-48 174
MsaG027638 MtrunA17_Chr4g0070811 47.945 219 99 5 1 206 1 217 6.13e-46 163
MsaG027638 MtrunA17_Chr3g0085901 47.343 207 100 4 1 206 95 293 1.77e-45 164
MsaG027638 MtrunA17_Chr7g0252201 51.955 179 79 3 1 173 100 277 2.06e-45 162
MsaG027638 MtrunA17_Chr7g0264171 31.156 398 186 15 244 570 37 417 5.03e-45 169
MsaG027638 MtrunA17_Chr3g0132661 40.152 264 139 8 1 256 109 361 9.84e-45 164
MsaG027638 MtrunA17_Chr7g0264161 33.228 316 173 12 248 541 2 301 6.73e-42 153
MsaG027638 MtrunA17_Chr8g0366411 43.810 210 110 6 1 206 163 368 1.19e-40 154
MsaG027638 MtrunA17_Chr5g0404531 50.877 171 74 5 37 206 2 163 3.92e-40 145
MsaG027638 MtrunA17_Chr2g0325081 44.749 219 106 5 1 206 97 313 1.58e-39 148
MsaG027638 MtrunA17_Chr3g0085981 43.810 210 107 5 1 206 1 203 4.99e-39 145
MsaG027638 MtrunA17_Chr5g0425651 62.500 112 41 1 453 564 1 111 1.50e-38 138
MsaG027638 MtrunA17_Chr3g0078191 44.292 219 106 6 1 206 131 346 1.77e-38 146
MsaG027638 MtrunA17_Chr6g0460041 47.305 167 85 3 6 171 130 294 2.32e-38 143
MsaG027638 MtrunA17_Chr3g0084251 44.340 212 107 5 3 206 286 494 4.88e-38 148
MsaG027638 MtrunA17_Chr3g0084251 45.070 142 74 2 1 138 110 251 3.07e-22 100
MsaG027638 MtrunA17_Chr4g0049041 43.836 219 106 7 1 206 108 322 4.97e-38 145
MsaG027638 MtrunA17_Chr5g0440681 38.976 254 123 5 3 256 108 329 5.29e-38 144
MsaG027638 MtrunA17_Chr4g0049111 43.192 213 110 6 1 206 126 334 8.50e-38 146
MsaG027638 MtrunA17_Chr3g0099431 48.387 155 75 4 54 206 5 156 1.64e-37 136
MsaG027638 MtrunA17_Chr3g0084261 45.540 213 100 7 1 206 113 316 1.69e-37 143
MsaG027638 MtrunA17_Chr3g0084241 46.226 212 103 6 1 206 92 298 3.34e-37 142
MsaG027638 MtrunA17_Chr3g0082431 44.444 207 107 7 3 205 96 298 2.46e-36 139
MsaG027638 MtrunA17_Chr2g0331741 43.836 219 107 7 1 206 163 378 1.27e-35 139
MsaG027638 MtrunA17_Chr3g0099441 35.507 276 115 7 6 231 116 378 1.35e-35 140
MsaG027638 MtrunA17_Chr3g0082451 43.961 207 107 8 3 205 96 297 4.65e-35 136
MsaG027638 MtrunA17_Chr6g0457621 39.819 221 110 6 2 206 113 326 5.53e-35 136
MsaG027638 MtrunA17_Chr3g0077781 41.935 217 109 6 1 204 100 312 5.66e-35 135
MsaG027638 MtrunA17_Chr2g0282961 28.723 376 232 13 237 590 52 413 6.21e-35 139
MsaG027638 MtrunA17_Chr3g0085951 43.810 210 105 6 3 206 88 290 6.55e-35 136
MsaG027638 MtrunA17_Chr3g0091161 40.952 210 113 5 1 206 99 301 8.58e-35 135
MsaG027638 MtrunA17_Chr4g0048151 40.930 215 112 6 1 206 113 321 1.08e-34 137
MsaG027638 MtrunA17_Chr6g0486911 39.423 208 119 5 2 206 108 311 1.50e-34 135
MsaG027638 MtrunA17_Chr2g0276781 28.871 381 226 10 243 597 28 389 3.12e-34 138
MsaG027638 MtrunA17_Chr4g0049531 41.204 216 112 6 1 206 41 251 3.19e-34 134
MsaG027638 MtrunA17_Chr8g0385791 29.444 360 231 9 224 569 49 399 4.07e-34 138
MsaG027638 MtrunA17_Chr3g0085961 42.857 217 103 6 1 206 98 304 4.09e-34 134
MsaG027638 MtrunA17_Chr7g0239061 28.800 375 240 11 225 585 5 366 5.12e-34 137
MsaG027638 MtrunA17_Chr4g0049121 39.301 229 116 7 1 209 115 340 6.96e-34 135
MsaG027638 MtrunA17_Chr4g0009751 28.895 353 231 8 244 584 25 369 1.67e-33 135
MsaG027638 MtrunA17_Chr6g0460081 41.361 191 99 4 22 206 143 326 1.93e-33 131
MsaG027638 MtrunA17_Chr2g0285461 30.286 350 215 11 243 569 28 371 6.55e-33 135
MsaG027638 MtrunA17_Chr4g0046371 29.810 369 212 15 237 577 37 386 6.57e-32 131
MsaG027638 MtrunA17_Chr8g0346711 29.897 388 238 12 229 599 24 394 9.11e-32 130
MsaG027638 MtrunA17_Chr6g0486921 35.931 231 119 5 2 206 105 332 1.01e-31 127
MsaG027638 MtrunA17_Chr4g0049071 35.338 266 151 7 1 256 133 387 3.56e-31 126
MsaG027638 MtrunA17_Chr2g0301251 30.409 342 201 14 243 561 28 355 8.40e-31 128
MsaG027638 MtrunA17_Chr1g0156321 30.357 336 210 11 243 561 48 376 1.42e-30 127
MsaG027638 MtrunA17_Chr4g0070181 29.240 342 205 10 243 561 28 355 1.66e-30 127
MsaG027638 MtrunA17_Chr5g0433301 29.762 336 213 10 243 561 29 358 1.67e-30 127
MsaG027638 MtrunA17_Chr8g0376771 28.889 360 206 13 243 569 28 370 6.32e-30 125
MsaG027638 MtrunA17_Chr5g0419301 37.850 214 120 7 1 206 113 321 1.41e-29 121
MsaG027638 MtrunA17_Chr3g0109391 29.464 336 214 10 243 561 31 360 1.43e-29 124
MsaG027638 MtrunA17_Chr8g0364381 37.104 221 119 5 5 206 155 374 3.77e-29 120
MsaG027638 MtrunA17_Chr5g0419311 37.559 213 120 7 1 205 113 320 4.47e-29 118
MsaG027638 MtrunA17_Chr4g0023631 40.385 208 114 5 5 206 103 306 5.07e-29 120
MsaG027638 MtrunA17_Chr4g0005311 42.857 203 110 4 6 206 114 312 9.40e-29 119
MsaG027638 MtrunA17_Chr4g0049091 37.946 224 113 7 1 206 12 227 2.14e-27 114
MsaG027638 MtrunA17_Chr3g0078891 38.839 224 111 9 5 206 100 319 6.03e-27 113
MsaG027638 MtrunA17_Chr4g0049051 46.154 130 66 3 76 205 1 126 1.89e-26 105
MsaG027638 MtrunA17_Chr4g0029671 27.500 400 229 13 237 597 53 430 1.54e-25 112
MsaG027638 MtrunA17_Chr8g0389081 28.614 339 213 11 243 561 37 366 2.75e-25 111
MsaG027638 MtrunA17_Chr3g0104731 42.286 175 90 5 1 165 103 276 2.76e-25 106
MsaG027638 MtrunA17_Chr3g0078731 37.719 228 112 10 5 206 100 323 6.21e-25 107
MsaG027638 MtrunA17_Chr3g0077791 37.004 227 123 5 1 209 102 326 8.82e-25 105
MsaG027638 MtrunA17_Chr1g0199541 40.000 165 91 4 1 159 109 271 8.94e-25 104
MsaG027638 MtrunA17_Chr2g0326281 28.325 406 242 14 237 602 55 451 1.74e-24 108
MsaG027638 MtrunA17_Chr3g0091331 46.324 136 70 3 72 206 15 148 6.14e-24 98.2
MsaG027638 MtrunA17_Chr3g0099461 30.466 279 89 5 6 231 116 342 7.82e-24 104
MsaG027638 MtrunA17_Chr8g0362531 36.364 209 115 8 6 201 132 335 1.71e-23 103
MsaG027638 MtrunA17_Chr3g0078681 37.500 216 105 10 5 194 114 325 2.84e-22 98.6
MsaG027638 MtrunA17_Chr4g0058061 34.259 216 129 7 9 218 246 454 4.72e-21 96.7
MsaG027638 MtrunA17_Chr4g0011401 32.751 229 137 8 6 222 232 455 3.21e-20 94.4
MsaG027638 MtrunA17_Chr3g0104721 39.375 160 89 4 4 156 118 276 5.37e-20 90.9
MsaG027638 MtrunA17_Chr6g0488401 36.150 213 115 6 6 204 100 305 5.68e-20 92.4
MsaG027638 MtrunA17_Chr7g0269551 41.732 127 70 3 57 183 1 123 9.99e-20 85.5
MsaG027638 MtrunA17_Chr7g0220351 32.407 216 130 6 6 205 234 449 3.48e-19 91.3
MsaG027638 MtrunA17_Chr3g0091211 34.715 193 75 6 1 188 99 245 3.98e-19 87.0
MsaG027638 MtrunA17_Chr4g0010971 36.458 192 99 7 26 206 49 228 1.53e-17 83.6
MsaG027638 MtrunA17_Chr6g0452031 32.367 207 122 8 6 199 116 317 3.67e-17 83.2
MsaG027638 MtrunA17_Chr8g0361351 33.178 214 122 9 6 204 103 310 5.81e-17 83.2
MsaG027638 MtrunA17_Chr4g0011331 33.171 205 124 7 6 205 240 436 7.60e-17 83.6
MsaG027638 MtrunA17_Chr6g0462351 40.496 121 68 3 74 194 6 122 1.23e-16 76.6
MsaG027638 MtrunA17_Chr4g0011321 30.769 195 127 5 6 196 94 284 1.41e-16 80.5
MsaG027638 MtrunA17_Chr1g0170171 36.216 185 93 9 34 209 120 288 3.58e-16 79.7
MsaG027638 MtrunA17_Chr3g0084231 42.400 125 67 3 87 209 127 248 1.24e-15 79.0
MsaG027638 MtrunA17_Chr8g0356211 26.496 234 137 10 351 566 9 225 2.99e-14 73.9
MsaG027638 MtrunA17_Chr4g0012101 35.714 168 95 6 34 197 58 216 3.85e-14 73.6
MsaG027638 MtrunA17_Chr4g0049141 37.903 124 63 5 94 206 2 122 1.34e-13 72.0
MsaG027638 MtrunA17_Chr4g0049081 43.902 82 43 2 125 206 3 81 1.76e-13 68.9
MsaG027638 MtrunA17_Chr2g0331731 40.984 122 61 4 94 206 2 121 2.03e-13 68.9
MsaG027638 MtrunA17_Chr2g0317201 33.333 180 109 6 34 211 15 185 3.11e-13 69.3
MsaG027638 MtrunA17_Chr1g0188701 29.167 168 97 6 4 153 91 254 3.12e-13 70.5
MsaG027638 MtrunA17_Chr5g0419861 35.897 117 69 3 276 386 12 128 5.41e-13 66.2
MsaG027638 MtrunA17_Chr4g0039521 40.909 110 59 3 34 138 109 217 9.49e-13 68.2
MsaG027638 MtrunA17_Chr3g0080921 47.887 71 36 1 1 70 47 117 1.30e-12 64.7
MsaG027638 MtrunA17_Chr1g0170181 29.834 181 116 6 35 210 25 199 2.32e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027638 AT1G77850.2 33.604 369 182 13 243 562 24 378 9.93e-54 193
MsaG027638 AT1G77850.1 33.604 369 182 13 243 562 24 378 1.29e-53 192
MsaG027638 AT4G30080.1 36.292 383 183 14 243 573 24 397 1.80e-50 186
MsaG027638 AT2G28350.2 33.679 386 181 14 243 566 15 387 1.15e-46 174
MsaG027638 AT2G28350.1 33.679 386 181 14 243 566 15 387 1.23e-46 175
MsaG027638 AT2G46530.3 31.285 358 219 9 243 584 47 393 1.30e-40 157
MsaG027638 AT2G46530.1 31.285 358 219 9 243 584 26 372 1.89e-40 156
MsaG027638 AT3G61830.1 29.563 389 234 12 220 589 15 382 2.83e-40 155
MsaG027638 AT1G59750.2 31.496 381 222 13 243 600 27 391 4.39e-37 147
MsaG027638 AT1G59750.4 32.011 353 206 12 243 577 27 363 1.43e-36 145
MsaG027638 AT1G59750.1 32.011 353 206 12 243 577 27 363 1.44e-36 145
MsaG027638 AT1G59750.3 32.011 353 206 12 243 577 27 363 1.44e-36 145
MsaG027638 AT1G34390.1 30.526 380 221 13 231 590 24 380 3.94e-36 143
MsaG027638 AT1G35520.1 31.282 390 207 16 231 590 24 382 1.11e-35 142
MsaG027638 AT1G34410.1 30.513 390 210 16 231 590 24 382 4.05e-34 137
MsaG027638 AT5G62000.4 29.243 383 230 12 243 601 66 431 7.91e-34 138
MsaG027638 AT5G62000.2 29.243 383 230 12 243 601 66 431 9.65e-34 137
MsaG027638 AT5G62000.5 29.243 383 230 12 243 601 66 431 9.65e-34 137
MsaG027638 AT5G62000.3 29.243 383 230 12 243 601 66 431 9.65e-34 137
MsaG027638 AT5G62000.1 29.243 383 230 12 243 601 66 431 9.65e-34 137
MsaG027638 AT1G35540.1 30.670 388 212 17 231 590 24 382 1.44e-33 136
MsaG027638 AT1G34310.1 31.503 346 187 13 242 561 31 352 3.21e-33 135
MsaG027638 AT5G37020.2 30.357 336 211 10 243 561 27 356 1.90e-32 133
MsaG027638 AT5G37020.1 30.357 336 211 10 243 561 27 356 2.33e-32 133
MsaG027638 AT1G35240.1 30.532 357 194 14 231 561 24 352 3.90e-32 131
MsaG027638 AT1G30330.1 28.852 357 211 11 243 569 28 371 3.95e-32 133
MsaG027638 AT1G30330.3 28.852 357 211 11 243 569 30 373 4.22e-32 133
MsaG027638 AT1G30330.2 28.852 357 211 11 243 569 30 373 4.22e-32 133
MsaG027638 AT4G23980.1 29.315 365 221 13 224 566 2 351 1.20e-31 130
MsaG027638 AT4G23980.2 29.315 365 221 13 224 566 2 351 1.23e-31 130
MsaG027638 AT1G34170.1 30.055 366 213 14 231 575 22 365 4.34e-30 124
MsaG027638 AT1G19220.1 29.379 354 211 12 233 561 16 355 5.63e-30 126
MsaG027638 AT3G61830.2 30.000 320 197 9 285 589 24 331 1.08e-29 124
MsaG027638 AT2G33860.1 29.188 394 224 15 237 604 53 417 5.46e-29 122
MsaG027638 AT1G19850.1 30.994 342 203 12 243 562 59 389 3.07e-28 120
MsaG027638 AT1G19850.2 30.994 342 203 12 243 562 48 378 3.29e-28 120
MsaG027638 AT5G20730.3 29.446 343 204 11 243 561 29 357 5.57e-28 120
MsaG027638 AT5G20730.1 29.446 343 204 11 243 561 29 357 6.24e-28 120
MsaG027638 AT5G20730.2 29.738 343 202 12 243 561 29 356 1.56e-27 119
MsaG027638 AT1G43950.1 32.701 211 118 7 231 429 24 222 4.77e-25 103
MsaG027638 AT1G34170.2 28.099 363 198 13 231 575 22 339 2.23e-24 107
MsaG027638 AT5G60450.1 28.902 346 209 13 243 561 70 405 2.99e-24 108
MsaG027638 AT1G34170.3 28.099 363 198 13 231 575 22 339 3.29e-24 107
MsaG027638 AT5G60450.2 28.902 346 209 13 243 561 46 381 3.30e-24 108
MsaG027638 AT2G46530.2 30.182 275 169 8 320 584 24 285 1.08e-23 105
MsaG027638 AT2G33860.2 29.329 283 160 12 338 604 2 260 5.14e-18 87.4
MsaG027638 AT5G37020.3 28.571 266 172 8 309 561 1 261 6.50e-18 88.2

Find 134 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAGTTTGTATTGTTAAAT+TGG 0.158810 5:+49369044 None:intergenic
TGTGGTAAATTCAGAGAATT+AGG 0.172442 5:+49369222 None:intergenic
AACTGATGAGATTAAGGTTT+TGG 0.252605 5:-49362619 MsaT027638.1:CDS
TGTATCCAACATCGTCTTCT+TGG 0.259399 5:+49362979 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5:+49363206 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5:-49362371 MsaT027638.1:CDS
GATGACAATTTCTCAGCTTA+AGG 0.289847 5:-49362418 MsaT027638.1:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5:-49362625 MsaT027638.1:CDS
CTCCTTTGTTTCTACTCAAC+TGG 0.299577 5:-49368858 MsaT027638.1:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.299743 5:+49361741 None:intergenic
TTTCCATATTGTCGAGTCTT+TGG 0.299860 5:+49364655 None:intergenic
ATTAAACAAATTGAATAGTT+TGG 0.306881 5:-49369127 MsaT027638.1:CDS
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5:+49361859 None:intergenic
GATCTTTCCCAAGCTTGATT+TGG 0.314401 5:-49362877 MsaT027638.1:CDS
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5:+49362357 None:intergenic
TAAACAAAGGTACAAAGATT+TGG 0.335008 5:+49368985 None:intergenic
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5:-49362489 MsaT027638.1:CDS
TTGAATAGTTTGGTCATTAC+CGG 0.341187 5:-49369117 MsaT027638.1:CDS
CTACTTCCCACGAGGTCATC+TGG 0.355762 5:-49363183 MsaT027638.1:CDS
TGACATTAACTTGTAATTGT+TGG 0.356752 5:+49369343 None:intergenic
TCGACCCTGCCAAGGTTTGT+TGG 0.360193 5:+49361740 None:intergenic
GTGCCAAAGACTCGACAATA+TGG 0.365007 5:-49364658 MsaT027638.1:CDS
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5:+49362358 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5:+49363221 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5:+49362491 None:intergenic
ATGACAATTTCTCAGCTTAA+GGG 0.392754 5:-49362417 MsaT027638.1:CDS
GCCAATTTAACAATACAAAC+TGG 0.404825 5:-49369045 MsaT027638.1:CDS
CATAAAGCTTGTTTAATGCT+TGG 0.405163 5:+49364632 None:intergenic
AGAGACAAGTTGGTTATTTG+TGG 0.416743 5:+49369204 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5:+49362662 None:intergenic
CAATTTGTTTAATGTCGAAA+AGG 0.424860 5:+49369137 None:intergenic
AATTCCGACAGATATCTTTC+CGG 0.425831 5:+49362695 None:intergenic
CTTGGTCAGTTACGGGAGTC+AGG 0.426329 5:+49363027 None:intergenic
CGACCTTAAAAGCCGAGAAT+GGG 0.441124 5:-49362829 MsaT027638.1:CDS
CTGAAATTTCATCTCAGACT+TGG 0.441890 5:+49369017 None:intergenic
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5:+49363226 None:intergenic
CCAAGGTTTGGCTATGTGTC+AGG 0.447799 5:+49361706 None:intergenic
AGTCACTTCAACAACTCTTA+AGG 0.451071 5:-49368803 MsaT027638.1:intron
GACTCGTCCAAATCAAGCTT+GGG 0.456946 5:+49362870 None:intergenic
GTGTATTATCCGACAGCTAT+TGG 0.461034 5:-49362555 MsaT027638.1:CDS
AATTTGTTTAATGTCGAAAA+GGG 0.462015 5:+49369138 None:intergenic
TTTGTTTCTACTCAACTGGC+TGG 0.464499 5:-49368854 MsaT027638.1:CDS
GACGGGAACTACAGGAGGAG+GGG 0.468379 5:+49363001 None:intergenic
TTTGTACCTTTGTTTATAGC+GGG 0.468458 5:-49368978 MsaT027638.1:CDS
TACGGGAGTCAGGAGCAGTT+TGG 0.469490 5:+49363037 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5:-49362501 MsaT027638.1:CDS
ACCTTTGTTTATAGCGGGCA+TGG 0.481035 5:-49368973 MsaT027638.1:CDS
AGTCTTTGGCACGAGTATAC+CGG 0.483848 5:+49364669 None:intergenic
ATAATCACGAACTATACAAC+CGG 0.486548 5:+49369098 None:intergenic
GATGAAAACAACAGAATCGT+TGG 0.490573 5:+49362728 None:intergenic
AGACTCGTCCAAATCAAGCT+TGG 0.490804 5:+49362869 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5:+49362450 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5:+49363143 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5:-49361901 MsaT027638.1:intron
CCGACCTTAAAAGCCGAGAA+TGG 0.494002 5:-49362830 MsaT027638.1:CDS
GACCCTGCCAAGGTTTGTTG+GGG 0.496074 5:+49361742 None:intergenic
GGCGGAGTTGACGGTGGAGG+AGG 0.496620 5:+49361801 None:intergenic
TAATGTGTTGCACGAAAAGA+AGG 0.504967 5:+49369293 None:intergenic
TGAGGCGGAGTTGACGGTGG+AGG 0.507469 5:+49361798 None:intergenic
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5:-49361861 MsaT027638.1:CDS
GTCAATTGTGAGTTTAAGAG+TGG 0.513556 5:+49369269 None:intergenic
CAACCTGCCAAGGATTGACT+AGG 0.513736 5:+49361854 None:intergenic
AAATCTTAATGTAGAAATGT+GGG 0.514840 5:-49368896 MsaT027638.1:CDS
GAGACGATGCATGGAATGGA+TGG 0.515792 5:+49363104 None:intergenic
TAAATCTTAATGTAGAAATG+TGG 0.517945 5:-49368897 MsaT027638.1:CDS
TCCAACATCGTCTTCTTGGA+CGG 0.518377 5:+49362983 None:intergenic
AATCTCAGTAGATTACATAC+TGG 0.519388 5:-49362786 MsaT027638.1:CDS
TCTTCTTGGACGGGAACTAC+AGG 0.522794 5:+49362993 None:intergenic
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5:-49362358 MsaT027638.1:intron
TGGACGGGAACTACAGGAGG+AGG 0.523890 5:+49362999 None:intergenic
CTCTCACAAAGTTTCTGTAA+AGG 0.526772 5:+49368949 None:intergenic
GGAAGAGACGATGCATGGAA+TGG 0.530637 5:+49363100 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5:+49363144 None:intergenic
TGACTAGGTTTGGATTCTGC+GGG 0.536979 5:+49361869 None:intergenic
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5:+49361766 None:intergenic
CTTTGTACCTTTGTTTATAG+CGG 0.540472 5:-49368979 MsaT027638.1:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5:-49362654 MsaT027638.1:CDS
AGATATCTGTCGGAATTCGC+AGG 0.543135 5:-49362689 MsaT027638.1:CDS
CCCTGCCAAGGTTTGTTGGG+GGG 0.543970 5:+49361744 None:intergenic
ACGGGAACTACAGGAGGAGG+GGG 0.544584 5:+49363002 None:intergenic
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5:-49362601 MsaT027638.1:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5:+49361844 None:intergenic
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5:-49361857 MsaT027638.1:CDS
GAGGAGGAAGTCAAAGGTGC+AGG 0.548119 5:+49361817 None:intergenic
ACGATGCCATGAAGATTGGT+TGG 0.558168 5:-49362497 MsaT027638.1:CDS
ACCCTGCCAAGGTTTGTTGG+GGG 0.559662 5:+49361743 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.560711 5:-49362782 MsaT027638.1:CDS
CCAACATCGTCTTCTTGGAC+GGG 0.562296 5:+49362984 None:intergenic
AAAACGATGTAGAGACAAGT+TGG 0.565476 5:+49369194 None:intergenic
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5:+49363145 None:intergenic
TATTGTTAAATTGGCTAGCG+TGG 0.572267 5:+49369053 None:intergenic
TTGACTAGGTTTGGATTCTG+CGG 0.581555 5:+49361868 None:intergenic
TGGACGGTAACGATCAAGAA+TGG 0.583957 5:+49363124 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5:-49362661 MsaT027638.1:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5:-49363245 MsaT027638.1:intron
GTGTTGCACGAAAAGAAGGA+AGG 0.590142 5:+49369297 None:intergenic
TGACAATTTCTCAGCTTAAG+GGG 0.599563 5:-49362416 MsaT027638.1:CDS
AGCCAGTTGAGTAGAAACAA+AGG 0.601356 5:+49368856 None:intergenic
TAATCTACTGAGATTTGCAT+AGG 0.604127 5:+49362794 None:intergenic
TGTTGCACGAAAAGAAGGAA+GGG 0.604225 5:+49369298 None:intergenic
TCAACGGAAGAGACGATGCA+TGG 0.604313 5:+49363095 None:intergenic
CCCGTCCAAGAAGACGATGT+TGG 0.606646 5:-49362984 MsaT027638.1:CDS
AAAGGTACAAAGATTTGGAG+TGG 0.607869 5:+49368990 None:intergenic
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5:+49363059 None:intergenic
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5:-49361736 MsaT027638.1:CDS
GACCTTAAAAGCCGAGAATG+GGG 0.616896 5:-49362828 MsaT027638.1:CDS
GGACGGGAACTACAGGAGGA+GGG 0.619395 5:+49363000 None:intergenic
CGATGCATGGAATGGATGGA+CGG 0.620709 5:+49363108 None:intergenic
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5:-49362651 MsaT027638.1:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5:+49363220 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5:+49363133 None:intergenic
TCTTGAGGCGGAGTTGACGG+TGG 0.632846 5:+49361795 None:intergenic
GAACAGATCGATGATCATGC+CGG 0.634054 5:-49364688 MsaT027638.1:CDS
TCACGGCAACCAATAGCTGT+CGG 0.635734 5:+49362546 None:intergenic
TTGTGTGAGAGTCTTGACAT+AGG 0.637006 5:+49362947 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5:+49361732 None:intergenic
GTTGCACGAAAAGAAGGAAG+GGG 0.642506 5:+49369299 None:intergenic
TCTGCCGGAAAGATATCTGT+CGG 0.642648 5:-49362699 MsaT027638.1:CDS
GCGTGGCATTCACTATGCAG+AGG 0.645448 5:+49369070 None:intergenic
CTTACAATCTGTAATCCCAA+AGG 0.649436 5:+49364601 None:intergenic
AGGATCAACAAAGAGATCAA+CGG 0.651533 5:+49363079 None:intergenic
ACCTTAAAAGCCGAGAATGG+GGG 0.652954 5:-49362827 MsaT027638.1:CDS
TGTTGATCCTCACACCGACG+AGG 0.655379 5:-49363066 MsaT027638.1:CDS
TAGCATCCACCACAAAATCA+CGG 0.661399 5:+49362529 None:intergenic
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5:+49363052 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5:+49363222 None:intergenic
ACCATGCCCGCTATAAACAA+AGG 0.666233 5:+49368972 None:intergenic
ATCAAGTTAGAGCATTCGAT+AGG 0.670484 5:+49362897 None:intergenic
TCTTGGACGGGAACTACAGG+AGG 0.671507 5:+49362996 None:intergenic
CATGTTCCAGATGACCTCGT+GGG 0.672658 5:+49363177 None:intergenic
GCATGTTCCAGATGACCTCG+TGG 0.672744 5:+49363176 None:intergenic
ACGGTGGAGGAGGAAGTCAA+AGG 0.680164 5:+49361811 None:intergenic
TTGCACGAAAAGAAGGAAGG+GGG 0.704391 5:+49369300 None:intergenic
AAAGTTTACTACTTCCCACG+AGG 0.761653 5:-49363191 MsaT027638.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 49361717 49369418 49361717 ID=MsaG027638
Chr5 mRNA 49361717 49369418 49361717 ID=MsaT027638.1;Parent=MsaG027638
Chr5 exon 49361717 49361914 49361717 ID=MsaT027638.1.exon4;Parent=MsaT027638.1
Chr5 CDS 49361717 49361914 49361717 ID=cds.MsaT027638.1;Parent=MsaT027638.1
Chr5 exon 49362359 49363255 49362359 ID=MsaT027638.1.exon3;Parent=MsaT027638.1
Chr5 CDS 49362359 49363255 49362359 ID=cds.MsaT027638.1;Parent=MsaT027638.1
Chr5 exon 49364606 49364713 49364606 ID=MsaT027638.1.exon2;Parent=MsaT027638.1
Chr5 CDS 49364606 49364713 49364606 ID=cds.MsaT027638.1;Parent=MsaT027638.1
Chr5 exon 49368804 49369418 49368804 ID=MsaT027638.1.exon1;Parent=MsaT027638.1
Chr5 CDS 49368804 49369418 49368804 ID=cds.MsaT027638.1;Parent=MsaT027638.1
Gene Sequence

>MsaT027638.1

ATGGACCCTCGCCTACTCAAAAGAATTTTGAAATATGCTTTCTCACACAATGTCCAACAATTACAAGTTAATGTCAAATGTTTTAGTCCACAATTTCCCCCTTCCTTCTTTTCGTGCAACACATTAACCACTCTTAAACTCACAATTGACTATTATTCTCATCAAAATTTATTTCCTAATTCTCTGAATTTACCACAAATAACCAACTTGTCTCTACATCGTTTTATCTTTTGTGTCGGCGATGATGGTAGTGTCAATCCCTTTTCGACATTAAACAAATTGAATAGTTTGGTCATTACCGGTTGTATAGTTCGTGATTATTTAAACCTCTGCATAGTGAATGCCACGCTAGCCAATTTAACAATACAAACTGGCTTTTCCAAGTCTGAGATGAAATTTCAGTTATCCACTCCAAATCTTTGTACCTTTGTTTATAGCGGGCATGGTCCTTTACAGAAACTTTGTGAGAGCAATATAAATCTCTCTTCTATCGAACATGTAAATCTTAATGTAGAAATGTGGGTAACTTACATAGAGTCTCCTTTGTTTCTACTCAACTGGCTGGCTGAGCTTGCAAATATCAAATCATTGTCAGTCACTTCAACAACTCTTAAGGTAGAACAGATCGATGATCATGCCGGTATACTCGTGCCAAAGACTCGACAATATGGAAACCAAGCATTAAACAAGCTTTATGAAAAAACACCTTTGGGATTACAGATTATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACGAGGTCATCTGGAACATGCATGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGTCCATCCATTCCATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGTCCAAGAAGACGATGTTGGATACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCTGATTGTGCAAGAGTATTATGCGTACCTATCGAATGCTCTAACTTGATCTTTCCCAAGCTTGATTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTTGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCCGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTATTATCCGACAGCTATTGGTTGCCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAGGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTTAACTGGGATGGACTTGATTTCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTTGACTTCCTCCTCCACCGTCAACTCCGCCTCAAGAAAAACTCCTAGACTTGCTGAGAGTTCATCCCCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGACACATAG
Protein sequence

>MsaT027638.1

MDPRLLKRILKYAFSHNVQQLQVNVKCFSPQFPPSFFSCNTLTTLKLTIDYYSHQNLFPNSLNLPQITNLSLHRFIFCVGDDGSVNPFSTLNKLNSLVITGCIVRDYLNLCIVNATLANLTIQTGFSKSEMKFQLSTPNLCTFVYSGHGPLQKLCESNINLSSIEHVNLNVEMWVTYIESPLFLLNWLAELANIKSLSVTSTTLKVEQIDDHAGILVPKTRQYGNQALNKLYEKTPLGLQIICAGPDVKIPKIHSKVYYFPRGHLEHACSSPTAATRTILDRYRPSIPCIVSSVDLFVDPHTDEVFAKLLLTPVTDQEPPPPVVPVQEDDVGYNLVSYVKTLTQSDCARVLCVPIECSNLIFPKLDLDESQSITVTDLKSREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTAIGCRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNLVNPWQVEVYNIPAPLTSSSTVNSASRKTPRLAESSSPQQTLAGSSGTLPDT*