AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027645

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027645 MtrunA17_Chr5g0424931 91.871 529 41 2 1 528 1 528 0.0 1000
MsaG027645 MtrunA17_Chr1g0178011 54.111 523 162 8 8 526 1 449 3.20e-177 506
MsaG027645 MtrunA17_Chr5g0417281 49.543 547 208 11 14 528 6 516 1.96e-157 459
MsaG027645 MtrunA17_Chr5g0436761 48.560 521 188 10 14 522 7 459 5.17e-153 445
MsaG027645 MtrunA17_Chr3g0115271 45.357 560 247 15 13 527 13 558 1.86e-144 428
MsaG027645 MtrunA17_Chr5g0424741 47.523 545 187 13 8 480 1 518 3.91e-143 422
MsaG027645 MtrunA17_Chr2g0277461 46.809 517 190 13 16 524 10 449 2.25e-133 395
MsaG027645 MtrunA17_Chr5g0425261 54.337 392 139 9 8 392 1 359 1.15e-130 385
MsaG027645 MtrunA17_Chr5g0424801 53.671 395 137 10 8 392 1 359 1.98e-130 384
MsaG027645 MtrunA17_Chr2g0289221 53.203 359 123 9 6 355 7 329 2.31e-117 350
MsaG027645 MtrunA17_Chr5g0417211 42.830 523 165 12 15 527 8 406 1.00e-115 348
MsaG027645 MtrunA17_Chr1g0181211 38.121 564 278 14 14 524 22 567 8.67e-102 321
MsaG027645 MtrunA17_Chr2g0326761 39.922 516 226 16 19 478 11 498 9.64e-100 316
MsaG027645 MtrunA17_Chr1g0199681 39.703 471 217 13 7 432 82 530 9.71e-96 305
MsaG027645 MtrunA17_Chr4g0028971 41.228 456 213 13 19 435 12 451 1.34e-95 305
MsaG027645 MtrunA17_Chr7g0264171 39.446 469 224 14 14 438 26 478 1.86e-95 302
MsaG027645 MtrunA17_Chr2g0277531 46.957 345 129 13 15 347 16 318 6.26e-87 271
MsaG027645 MtrunA17_Chr5g0425661 61.111 198 69 3 16 208 34 228 4.00e-79 248
MsaG027645 MtrunA17_Chr7g0264161 40.181 331 155 9 29 347 2 301 8.54e-70 226
MsaG027645 MtrunA17_Chr2g0282961 34.704 389 193 10 1 367 38 387 2.16e-65 223
MsaG027645 MtrunA17_Chr2g0276781 35.356 379 207 7 19 386 23 374 2.69e-61 213
MsaG027645 MtrunA17_Chr2g0285461 35.910 401 206 12 6 385 6 376 1.00e-60 215
MsaG027645 MtrunA17_Chr8g0385791 34.456 386 202 10 19 386 59 411 2.87e-60 213
MsaG027645 MtrunA17_Chr4g0009751 35.079 382 205 10 19 386 19 371 7.16e-60 208
MsaG027645 MtrunA17_Chr4g0046371 34.677 372 187 9 19 367 38 376 1.14e-59 209
MsaG027645 MtrunA17_Chr4g0070181 35.389 373 191 8 14 369 18 357 1.16e-59 213
MsaG027645 MtrunA17_Chr2g0301251 34.595 370 198 8 14 369 18 357 7.15e-59 211
MsaG027645 MtrunA17_Chr5g0433301 34.615 390 202 11 16 385 21 377 4.11e-58 207
MsaG027645 MtrunA17_Chr3g0109391 34.103 390 204 10 16 385 23 379 9.53e-58 206
MsaG027645 MtrunA17_Chr1g0156321 35.135 370 193 9 14 367 38 376 1.51e-57 206
MsaG027645 MtrunA17_Chr8g0376771 35.572 402 205 13 6 385 6 375 2.77e-57 205
MsaG027645 MtrunA17_Chr7g0239061 31.969 391 224 10 19 396 14 375 4.46e-56 199
MsaG027645 MtrunA17_Chr8g0389081 34.417 369 200 9 16 370 29 369 9.48e-56 202
MsaG027645 MtrunA17_Chr8g0346711 32.102 433 225 14 19 439 29 404 1.67e-54 194
MsaG027645 MtrunA17_Chr2g0326281 32.840 405 206 13 19 389 56 428 2.28e-54 196
MsaG027645 MtrunA17_Chr4g0029671 35.459 392 200 11 19 386 54 416 4.95e-53 192
MsaG027645 MtrunA17_Chr5g0425651 74.107 112 25 1 264 371 1 112 4.57e-51 170
MsaG027645 MtrunA17_Chr5g0424791 47.682 151 63 5 68 213 12 151 7.48e-32 120
MsaG027645 MtrunA17_Chr8g0356211 27.160 243 153 5 125 367 2 220 2.75e-22 97.8
MsaG027645 MtrunA17_Chr5g0419861 39.130 115 57 4 57 163 12 121 7.08e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027645 AT1G77850.1 39.092 573 287 12 1 527 1 557 1.12e-122 372
MsaG027645 AT1G77850.2 39.092 573 287 12 1 527 1 557 1.38e-122 372
MsaG027645 AT4G30080.1 39.312 552 277 18 19 527 19 555 4.76e-98 311
MsaG027645 AT2G28350.2 39.440 464 208 13 19 432 10 450 2.09e-89 288
MsaG027645 AT2G28350.1 39.440 464 208 13 19 432 10 450 4.05e-89 288
MsaG027645 AT2G46530.1 36.915 363 190 8 18 370 20 353 1.48e-65 223
MsaG027645 AT2G46530.3 36.944 360 188 8 18 367 41 371 2.57e-65 223
MsaG027645 AT3G61830.1 34.840 376 200 9 15 382 21 359 1.85e-62 215
MsaG027645 AT2G33860.1 34.865 370 185 8 19 367 54 388 1.17e-61 213
MsaG027645 AT1G30330.3 35.733 389 203 10 14 385 20 378 1.07e-60 215
MsaG027645 AT1G30330.2 35.733 389 203 10 14 385 20 378 1.07e-60 215
MsaG027645 AT1G30330.1 35.733 389 203 10 14 385 18 376 1.44e-60 215
MsaG027645 AT5G37020.2 34.158 404 214 11 16 400 19 389 9.81e-60 211
MsaG027645 AT5G37020.1 34.158 404 214 11 16 400 19 389 1.86e-59 211
MsaG027645 AT4G23980.2 30.801 487 279 13 19 484 12 461 1.59e-58 205
MsaG027645 AT5G60450.2 34.694 392 201 13 13 383 35 392 3.25e-58 206
MsaG027645 AT1G59750.4 30.667 525 298 16 19 519 22 504 5.05e-58 204
MsaG027645 AT1G59750.1 30.000 530 300 15 19 519 22 509 8.09e-58 204
MsaG027645 AT1G59750.3 30.000 530 300 15 19 519 22 509 8.09e-58 204
MsaG027645 AT5G60450.1 34.704 389 199 13 16 383 62 416 9.08e-58 206
MsaG027645 AT1G59750.2 33.791 364 204 6 19 372 22 358 1.03e-57 204
MsaG027645 AT5G62000.4 33.333 399 221 10 3 386 45 413 1.05e-57 206
MsaG027645 AT5G62000.2 32.840 405 215 9 3 386 45 413 1.27e-57 206
MsaG027645 AT5G62000.5 32.840 405 215 9 3 386 45 413 1.27e-57 206
MsaG027645 AT5G62000.3 32.840 405 215 9 3 386 45 413 1.27e-57 206
MsaG027645 AT5G62000.1 32.840 405 215 9 3 386 45 413 1.27e-57 206
MsaG027645 AT4G23980.1 33.679 386 211 9 19 390 12 366 2.70e-57 202
MsaG027645 AT5G20730.2 34.667 375 191 9 14 369 19 358 5.17e-57 206
MsaG027645 AT1G19220.1 34.015 391 203 10 16 386 20 375 1.09e-56 205
MsaG027645 AT5G20730.3 34.574 376 191 10 14 369 19 359 2.39e-55 201
MsaG027645 AT5G20730.1 34.574 376 191 10 14 369 19 359 2.68e-55 201
MsaG027645 AT1G34310.1 32.902 386 215 9 16 390 24 376 8.98e-55 194
MsaG027645 AT1G34390.1 32.216 388 215 10 19 398 27 374 9.18e-55 194
MsaG027645 AT1G19850.2 35.054 368 193 11 16 367 40 377 1.60e-54 197
MsaG027645 AT1G19850.1 35.054 368 193 11 16 367 51 388 2.20e-54 197
MsaG027645 AT1G34410.1 31.313 396 231 9 19 406 27 389 4.42e-54 192
MsaG027645 AT1G35520.1 32.898 383 213 10 19 390 27 376 8.49e-54 191
MsaG027645 AT1G35540.1 32.021 381 219 9 19 390 27 376 2.60e-52 188
MsaG027645 AT1G35240.1 31.783 387 212 11 19 390 27 376 1.18e-50 183
MsaG027645 AT3G61830.2 34.234 333 178 8 54 382 13 308 2.76e-48 176
MsaG027645 AT1G34170.1 27.833 503 269 16 19 487 25 467 1.89e-46 170
MsaG027645 AT2G46530.2 36.822 258 137 4 114 370 34 266 5.41e-45 166
MsaG027645 AT2G33860.2 34.231 260 132 4 117 367 2 231 2.57e-44 162
MsaG027645 AT1G34170.3 26.186 527 266 17 19 508 25 465 5.07e-41 155
MsaG027645 AT1G34170.2 26.640 503 249 16 19 487 25 441 5.18e-40 151
MsaG027645 AT5G37020.3 33.788 293 156 8 117 400 31 294 6.15e-40 154
MsaG027645 AT1G43950.1 38.725 204 111 5 19 216 27 222 2.54e-37 137
MsaG027645 ATMG00940.1 35.821 134 73 5 204 328 1 130 1.51e-15 74.7

Find 146 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5:-49472857 MsaT027645.1:CDS
GGCACATGTCTGCCAGATTT+TGG 0.193688 5:+49475974 None:intergenic
TTCAAACTACTAATGTTATT+TGG 0.205378 5:+49472935 None:intergenic
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5:-49475010 MsaT027645.1:intron
CATGATCATTTATCTCTATC+AGG 0.276134 5:-49473043 MsaT027645.1:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5:-49475191 MsaT027645.1:CDS
AATCAAAGGCAGTGAATAAT+AGG 0.317388 5:-49475353 MsaT027645.1:CDS
CAAACAATCGCCGTTGTTGA+TGG 0.320308 5:-49475861 MsaT027645.1:CDS
AACCGCAATTCCACAATGTT+TGG 0.327821 5:+49473095 None:intergenic
GTCAATGCATCAAATTTCAT+TGG 0.331745 5:+49472763 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5:-49475548 MsaT027645.1:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 5:+49475764 None:intergenic
ATTAGTAGTTTGAACACCTT+TGG 0.337278 5:-49472926 MsaT027645.1:CDS
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5:-49475129 MsaT027645.1:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5:+49475009 None:intergenic
TGCGTCAATAACCGCCTTCT+CGG 0.347408 5:+49475251 None:intergenic
ACGGCGATTGTTTGAGCGTT+AGG 0.351167 5:+49475870 None:intergenic
AGTTCTTGTCCTTCAGTTAC+CGG 0.352672 5:+49475747 None:intergenic
AATTTCATTGGATCCCTTAG+AGG 0.360856 5:+49472775 None:intergenic
GAAAGTGATGTCCATGATTC+TGG 0.375162 5:-49472815 MsaT027645.1:CDS
CTGGCTCCCTGCATGCCTCC+TGG 0.375810 5:+49473067 None:intergenic
GGAGAATTTAAGTCAAGCTT+GGG 0.377595 5:+49475597 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5:+49475010 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5:+49475933 None:intergenic
GTTTGGTTCAAAGATCTCAT+AGG 0.385258 5:+49473112 None:intergenic
CGGCGATTGTTTGAGCGTTA+GGG 0.387251 5:+49475871 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 5:+49475487 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5:-49473010 MsaT027645.1:CDS
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 5:-49475226 MsaT027645.1:CDS
GGGACAGACATGGTGGCCGG+TGG 0.403787 5:+49476017 None:intergenic
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5:-49475023 MsaT027645.1:CDS
CAGGAGGCATGCAGGGAGCC+AGG 0.425900 5:-49473066 MsaT027645.1:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5:+49473015 None:intergenic
ATCAAAACCGCGTCAATCCT+TGG 0.437926 5:-49473267 MsaT027645.1:CDS
CAAGGGAGAAGATTCCTCTA+AGG 0.441291 5:-49472789 MsaT027645.1:CDS
GCCGCGTGAAATCCAAAATC+TGG 0.443335 5:-49475986 MsaT027645.1:CDS
TTGAAGTTATATATTACCCT+AGG 0.447607 5:-49475200 MsaT027645.1:CDS
TCTTCTTCATCAACAACCTC+AGG 0.455552 5:+49475723 None:intergenic
AGGAGAATTTAAGTCAAGCT+TGG 0.457295 5:+49475596 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5:-49475766 MsaT027645.1:CDS
TTAGTAGTTTGAACACCTTT+GGG 0.458729 5:-49472925 MsaT027645.1:CDS
ACAAAATCTGATTCTAATAA+CGG 0.462788 5:-49475663 MsaT027645.1:CDS
GAACTCTATCATAGGAATGA+AGG 0.465037 5:+49473135 None:intergenic
TTGGATTTCACGCGGCGTGC+CGG 0.466164 5:+49475993 None:intergenic
AACACAAAAGAAGTTGGTTC+TGG 0.471305 5:-49472875 MsaT027645.1:CDS
CTCATAGGGAACTCTATCAT+AGG 0.480052 5:+49473127 None:intergenic
TGAAGAAGAAGACGGTGATA+AGG 0.483386 5:-49475709 MsaT027645.1:CDS
GTGGATGATGCTATGAAGAT+TGG 0.483610 5:-49475141 MsaT027645.1:CDS
TTCCTTTCCTTGTATCATCA+CGG 0.485412 5:-49475829 MsaT027645.1:CDS
TTACTTCCCACAAGGTCACT+TGG 0.494429 5:-49475910 MsaT027645.1:CDS
TGGAACGTTCACCGATGGAC+CGG 0.495600 5:+49475953 None:intergenic
GGCCACCATGTCTGTCCCTC+CGG 0.501925 5:-49476012 MsaT027645.1:CDS
GGTTATTGACGCAGTGGAGT+TGG 0.506246 5:-49475241 MsaT027645.1:CDS
GGGGATGAGGGTAAAGAGAA+TGG 0.508401 5:-49475327 MsaT027645.1:CDS
GATTTCACGCGGCGTGCCGG+AGG 0.509842 5:+49475996 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 5:+49475948 None:intergenic
CTCCCTTGCTACCAGAATCA+TGG 0.511654 5:+49472804 None:intergenic
TTTGGTTCAAAGATCTCATA+GGG 0.511861 5:+49473113 None:intergenic
TTCCGCCATGTCTTCCCCAC+CGG 0.514610 5:-49476033 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 5:-49475972 MsaT027645.1:CDS
TGAGGGTAAAGAGAATGGTT+TGG 0.516087 5:-49475322 MsaT027645.1:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5:+49476018 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5:-49475964 MsaT027645.1:CDS
AACTCTATCATAGGAATGAA+GGG 0.519149 5:+49473136 None:intergenic
GGTGGCCGGTGGGGAAGACA+TGG 0.519543 5:+49476028 None:intergenic
ATTGTTTGAGCGTTAGGGGA+AGG 0.521449 5:+49475876 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 5:-49475408 MsaT027645.1:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 5:-49475657 MsaT027645.1:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 5:+49475090 None:intergenic
GAGAGTTCCTGAGACGGAAA+AGG 0.524822 5:+49475576 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5:+49476019 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.529298 5:+49473184 None:intergenic
GACCTCATCGGTGTGAGGAT+CGG 0.532501 5:+49475791 None:intergenic
AACAGCAACACAAAAGAAGT+TGG 0.534960 5:-49472881 MsaT027645.1:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5:+49476014 None:intergenic
GGTTTGGAGGTCAAAGGAGA+GGG 0.536796 5:-49475306 MsaT027645.1:CDS
AGAAATGGTAGATGCACTGC+AGG 0.541883 5:+49473220 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 5:+49475404 None:intergenic
CATGTTCCAAGTGACCTTGT+GGG 0.545385 5:+49475904 None:intergenic
TTGTTTGAGCGTTAGGGGAA+GGG 0.546169 5:+49475877 None:intergenic
AAGGGAGAAGATTCCTCTAA+GGG 0.546926 5:-49472788 MsaT027645.1:CDS
TATTGACGCAGTGGAGTTGG+CGG 0.549772 5:-49475238 MsaT027645.1:CDS
TGGTTTGGAGGTCAAAGGAG+AGG 0.550776 5:-49475307 MsaT027645.1:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.551283 5:+49473250 None:intergenic
TAGAGATAAATGATCATGCC+TGG 0.553837 5:+49473048 None:intergenic
GTCCATGATTCTGGTAGCAA+GGG 0.557565 5:-49472806 MsaT027645.1:CDS
TCAACGGCCGTGATGATACA+AGG 0.557986 5:+49475822 None:intergenic
AACCAAACATTGTGGAATTG+CGG 0.559556 5:-49473097 MsaT027645.1:CDS
TTGAGCGTTAGGGGAAGGGC+AGG 0.559568 5:+49475881 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 5:-49475137 MsaT027645.1:CDS
TAGATGCACTGCAGGATTGT+GGG 0.562741 5:+49473228 None:intergenic
GAGAATGGTTTGGAGGTCAA+AGG 0.563275 5:-49475312 MsaT027645.1:CDS
TGAAGTTATATATTACCCTA+GGG 0.565837 5:-49475199 MsaT027645.1:CDS
GTTTACTGCAGCTGAATCAA+AGG 0.566092 5:-49475367 MsaT027645.1:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5:+49472955 None:intergenic
GGCGATTGTTTGAGCGTTAG+GGG 0.568994 5:+49475872 None:intergenic
GCATGTTCCAAGTGACCTTG+TGG 0.569473 5:+49475903 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.569581 5:-49475319 MsaT027645.1:CDS
ATTTCACGCGGCGTGCCGGA+GGG 0.569870 5:+49475997 None:intergenic
AAATTGCACCAATTAGCCCT+AGG 0.571867 5:+49475183 None:intergenic
CAGTAAACTTCATGTTTCTG+CGG 0.572135 5:+49475382 None:intergenic
TGTAGTAGATGCAAATGTTG+TGG 0.577875 5:-49475160 MsaT027645.1:CDS
CTACTCAGTCCGGTAACTGA+AGG 0.578098 5:-49475756 MsaT027645.1:CDS
TGATGCTATGAAGATTGGTT+GGG 0.580467 5:-49475136 MsaT027645.1:CDS
AAAGGCAGTGAATAATAGGA+AGG 0.580552 5:-49475349 MsaT027645.1:CDS
TGTCCATGATTCTGGTAGCA+AGG 0.582395 5:-49472807 MsaT027645.1:CDS
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5:+49472956 None:intergenic
CATGCGCCAATTAGGGACAC+TGG 0.600814 5:+49475017 None:intergenic
GATCTTTGAACCAAACATTG+TGG 0.601993 5:-49473105 MsaT027645.1:CDS
AGGCAGTGAATAATAGGAAG+GGG 0.602083 5:-49475347 MsaT027645.1:CDS
TCCATCGGTGAACGTTCCAA+AGG 0.605082 5:-49475949 MsaT027645.1:CDS
CTCAACAAAATCTATCCCAA+AGG 0.608187 5:+49472910 None:intergenic
GGCCGTGATGATACAAGGAA+AGG 0.610730 5:+49475827 None:intergenic
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5:-49475525 MsaT027645.1:CDS
GTAGATGCACTGCAGGATTG+TGG 0.617995 5:+49473227 None:intergenic
AAGGCAGTGAATAATAGGAA+GGG 0.619996 5:-49475348 MsaT027645.1:CDS
CAACTTGCCAAGGATTGACG+CGG 0.626664 5:+49473260 None:intergenic
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5:+49475779 None:intergenic
AATTGCACCAATTAGCCCTA+GGG 0.639117 5:+49475184 None:intergenic
AAGGGGAATACTAACCGAGA+AGG 0.642227 5:-49475265 MsaT027645.1:CDS
GTGATGATACAAGGAAAGGA+AGG 0.642933 5:+49475831 None:intergenic
GCGTGCCGGAGGGACAGACA+TGG 0.644657 5:+49476007 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 5:+49475624 None:intergenic
TCCGTCATGAACATCAGTAA+CGG 0.649838 5:+49475554 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5:+49475786 None:intergenic
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5:-49475524 MsaT027645.1:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5:-49475555 MsaT027645.1:CDS
CATCGGTGTGAGGATCGGCT+AGG 0.655915 5:+49475796 None:intergenic
AGCCGATCCTCACACCGATG+AGG 0.659256 5:-49475793 MsaT027645.1:CDS
AAAGTTTATTACTTCCCACA+AGG 0.663461 5:-49475918 MsaT027645.1:CDS
GGGAATACTAACCGAGAAGG+CGG 0.664523 5:-49475262 MsaT027645.1:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5:+49475042 None:intergenic
GAAGGCGGTTATTGACGCAG+TGG 0.667200 5:-49475247 MsaT027645.1:CDS
GGCAGTGAATAATAGGAAGG+GGG 0.673747 5:-49475346 MsaT027645.1:CDS
ACTCTATCATAGGAATGAAG+GGG 0.674109 5:+49473137 None:intergenic
TGATACAAGGAAAGGAAGGA+CGG 0.677912 5:+49475835 None:intergenic
TGCCGGAGGGACAGACATGG+TGG 0.679386 5:+49476010 None:intergenic
GTTGTTGATGAAGAAGAAGA+CGG 0.680880 5:-49475717 MsaT027645.1:CDS
ACTCTTGTTATCACTAAACA+AGG 0.684372 5:+49473162 None:intergenic
TGAAGGACAAGAACTTCCTG+AGG 0.686819 5:-49475739 MsaT027645.1:CDS
GTAACGGAGAGTTCCTGAGA+CGG 0.687969 5:+49475570 None:intergenic
GAGAATTTAAGTCAAGCTTG+GGG 0.690174 5:+49475598 None:intergenic
AGGATCGGCTAGGAGATCAA+CGG 0.692255 5:+49475806 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 5:-49475660 MsaT027645.1:CDS
GGAAGATCAAGTCAGCACAA+AGG 0.715164 5:+49475619 None:intergenic
AGGACGGTAACCATCAACAA+CGG 0.747640 5:+49475851 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 49472772 49476048 49472772 ID=MsaG027645
Chr5 mRNA 49472772 49476048 49472772 ID=MsaT027645.1;Parent=MsaG027645
Chr5 exon 49472772 49473320 49472772 ID=MsaT027645.1.exon2;Parent=MsaT027645.1
Chr5 CDS 49472772 49473320 49472772 ID=cds.MsaT027645.1;Parent=MsaT027645.1
Chr5 exon 49475011 49476048 49475011 ID=MsaT027645.1.exon1;Parent=MsaT027645.1
Chr5 CDS 49475011 49476048 49475011 ID=cds.MsaT027645.1;Parent=MsaT027645.1
Gene Sequence

>MsaT027645.1

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCACGCCGCGTGAAATCCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACAAGGTCACTTGGAACATGCCTGCCCTTCCCCTAACGCTCAAACAATCGCCGTTGTTGATGGTTACCGTCCTTCCTTTCCTTGTATCATCACGGCCGTTGATCTCCTAGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGAAGGACAAGAACTTCCTGAGGTTGTTGATGAAGAAGAAGACGGTGATAAGGTTGTTTCCTTCGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCCAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTACTGCAGCTGAATCAAAGGCAGTGAATAATAGGAAGGGGGATGAGGGTAAAGAGAATGGTTTGGAGGTCAAAGGAGAGGGTTTTTCTCGAGGCGGTAGAAGGGGAATACTAACCGAGAAGGCGGTTATTGACGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTAGATGCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCAAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTTAATTGGGATGAACTTGAAATCTTGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCACAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAACCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGAGTGACCCCTTCATTCCTATGATAGAGTTCCCTATGAGATCTTTGAACCAAACATTGTGGAATTGCGGTTATTTTCCAGGAGGCATGCAGGGAGCCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACATTAGTAGTTTGAACACCTTTGGGATAGATTTTGTTGAGACTCACAACAGCAACACAAAAGAAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACTGTTGAAAGTGATGTCCATGATTCTGGTAGCAAGGGAGAAGATTCCTCTAAGGGATCCAATGAAATTTGA
Protein sequence

>MsaT027645.1

MSSPPATMSVPPARRVKSKIWQTCAGPSVNVPKVRSKVYYFPQGHLEHACPSPNAQTIAVVDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTEGQELPEVVDEEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFTAAESKAVNNRKGDEGKENGLEVKGEGFSRGGRRGILTEKAVIDAVELAEKNMAFEVIYYPRANWCNFVVDANVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEILQNQNRVNPWQVELISHNPAVHLPFLSTKKPRLVQDSTLFSDNKSDPFIPMIEFPMRSLNQTLWNCGYFPGGMQGARHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNISSLNTFGIDFVETHNSNTKEVGSGSIKLFGKIIQTVESDVHDSGSKGEDSSKGSNEI*