AlfalfaGEDB | MsaG027697

M. sativa ssp. caerulea / MsaG027697

KEGG: -
GO: GO:0000976 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010208 GO:0010468 GO:0010556 GO:0010927 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0022622 GO:0023052 GO:0030198 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033036 GO:0033037 GO:0042221 GO:0042545 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044085 GO:0044212 GO:0044424 GO:0044464 GO:0045229 GO:0048229 GO:0048364 GO:0048646 GO:0048731 GO:0048830 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051252 GO:0051641 GO:0051716 GO:0052386 GO:0052543 GO:0052545 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071554 GO:0071555 GO:0071840 GO:0080090 GO:0085029 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027697	XP_024640663.1	80.321	249	16	2	46	271	9	247	5.31e-132	390

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027697	sp\|Q84WU6\|ARFQ_ARATH	45.366	205	79	6	76	252	20	219	1.07e-44	162

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027697	tr\|G7KAA1\|G7KAA1_MEDTR	81.982	222	17	1	45	243	8	229	1.84e-124	364

TFs/TRs:

Gene ID	Type	Classification
MsaG027697	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG003294	MsaG027697	0.804591	3.387840e-49	5.087548e-47
MsaG027697	MsaG030749	0.800314	2.553929e-48	3.477577e-46
MsaG038166	MsaG027697	0.805427	2.269631e-49	3.475124e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027697	MtrunA17_Chr5g0425661	81.982	222	17	1	45	243	7	228	4.55e-128	363
MsaG027697	MtrunA17_Chr1g0178011	52.966	236	69	5	75	271	12	244	5.91e-68	218
MsaG027697	MtrunA17_Chr5g0424931	45.185	270	81	5	72	280	16	279	1.25e-63	208
MsaG027697	MtrunA17_Chr2g0289221	48.261	230	84	7	75	271	20	247	4.03e-57	187
MsaG027697	MtrunA17_Chr5g0424741	45.255	274	81	9	72	280	9	278	1.02e-53	182
MsaG027697	MtrunA17_Chr5g0424801	46.186	236	76	8	75	271	12	235	3.82e-52	175
MsaG027697	MtrunA17_Chr5g0417281	42.500	240	104	5	72	280	8	244	5.72e-52	177
MsaG027697	MtrunA17_Chr5g0425261	46.610	236	75	8	75	271	12	235	2.38e-51	172
MsaG027697	MtrunA17_Chr5g0417211	45.887	231	89	5	75	271	12	240	2.59e-51	173
MsaG027697	MtrunA17_Chr3g0115271	38.075	239	126	3	67	284	11	248	4.57e-45	160
MsaG027697	MtrunA17_Chr2g0277461	43.103	232	95	5	75	269	13	244	8.68e-45	157
MsaG027697	MtrunA17_Chr2g0326761	40.594	202	100	3	71	252	7	208	4.72e-44	159
MsaG027697	MtrunA17_Chr5g0436761	41.250	240	104	7	75	280	12	248	1.28e-42	151
MsaG027697	MtrunA17_Chr2g0277531	40.773	233	94	9	75	269	20	246	1.18e-41	145
MsaG027697	MtrunA17_Chr4g0028971	41.379	203	89	4	75	252	12	209	9.75e-40	147
MsaG027697	MtrunA17_Chr1g0199681	40.000	205	97	5	72	252	91	293	7.04e-39	144
MsaG027697	MtrunA17_Chr1g0181211	37.438	203	102	2	75	252	27	229	4.95e-38	142
MsaG027697	MtrunA17_Chr7g0264161	33.937	221	110	3	85	269	2	222	2.88e-34	126
MsaG027697	MtrunA17_Chr7g0264171	33.654	208	109	4	71	252	27	231	2.41e-33	128
MsaG027697	MtrunA17_Chr8g0385791	35.122	205	105	4	75	252	59	262	1.39e-31	124
MsaG027697	MtrunA17_Chr2g0282961	33.962	212	108	3	71	252	49	258	1.40e-31	123
MsaG027697	MtrunA17_Chr2g0285461	33.047	233	118	5	74	275	22	247	1.78e-31	123
MsaG027697	MtrunA17_Chr2g0276781	34.975	203	107	3	75	252	23	225	1.96e-31	123
MsaG027697	MtrunA17_Chr4g0009751	34.483	203	108	3	75	252	19	221	4.38e-31	122
MsaG027697	MtrunA17_Chr8g0346711	34.975	203	107	3	75	252	29	231	4.55e-31	122
MsaG027697	MtrunA17_Chr7g0239061	32.836	201	110	2	75	250	14	214	1.32e-30	120
MsaG027697	MtrunA17_Chr5g0433301	32.618	233	119	5	74	275	23	248	1.04e-29	118
MsaG027697	MtrunA17_Chr8g0376771	33.649	211	110	3	72	254	20	228	1.41e-29	118
MsaG027697	MtrunA17_Chr4g0046371	32.093	215	109	4	75	254	38	250	2.11e-29	117
MsaG027697	MtrunA17_Chr1g0156321	34.112	214	115	5	71	258	39	252	2.76e-29	117
MsaG027697	MtrunA17_Chr3g0109391	31.760	233	121	5	74	275	25	250	5.15e-29	116
MsaG027697	MtrunA17_Chr2g0301251	32.489	237	122	6	46	254	2	228	8.91e-28	112
MsaG027697	MtrunA17_Chr4g0070181	32.857	210	111	5	71	252	19	226	4.27e-27	110
MsaG027697	MtrunA17_Chr8g0389081	29.439	214	124	2	72	258	29	242	2.23e-26	108
MsaG027697	MtrunA17_Chr2g0326281	29.825	228	112	5	75	254	56	283	1.62e-24	103
MsaG027697	MtrunA17_Chr4g0029671	30.516	213	113	3	75	252	54	266	3.21e-24	102
MsaG027697	MtrunA17_Chr5g0424791	40.909	132	67	4	117	248	31	151	5.88e-21	87.0
MsaG027697	MtrunA17_Chr8g0356211	41.772	79	46	0	174	252	16	94	2.77e-14	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027697	AT1G77850.1	45.366	205	79	6	76	252	20	219	1.08e-45	162
MsaG027697	AT1G77850.2	45.366	205	79	6	76	252	20	219	1.18e-45	162
MsaG027697	AT2G28350.2	35.443	237	96	4	71	264	6	228	1.62e-38	143
MsaG027697	AT2G28350.1	36.052	233	92	4	75	264	10	228	1.85e-38	143
MsaG027697	AT4G30080.1	38.725	204	97	5	75	252	19	220	2.09e-38	143
MsaG027697	AT3G61830.1	35.122	205	107	3	74	252	24	228	8.22e-34	130
MsaG027697	AT2G46530.1	35.122	205	107	3	74	252	20	224	1.60e-33	129
MsaG027697	AT2G46530.3	35.122	205	107	3	74	252	41	245	1.76e-33	129
MsaG027697	AT4G23980.2	34.634	205	107	3	75	252	12	216	1.75e-32	126
MsaG027697	AT4G23980.1	34.634	205	107	3	75	252	12	216	1.76e-32	126
MsaG027697	AT1G34310.1	37.500	200	103	4	75	252	27	226	2.28e-32	125
MsaG027697	AT2G33860.1	33.484	221	114	5	75	265	54	271	5.64e-32	124
MsaG027697	AT1G35540.1	36.946	203	105	5	75	255	27	228	1.29e-31	124
MsaG027697	AT1G34390.1	34.975	203	107	6	75	255	27	226	1.46e-31	123
MsaG027697	AT5G62000.2	32.843	204	111	2	75	252	61	264	5.42e-31	122
MsaG027697	AT5G62000.5	32.843	204	111	2	75	252	61	264	5.42e-31	122
MsaG027697	AT5G62000.3	32.843	204	111	2	75	252	61	264	5.42e-31	122
MsaG027697	AT5G62000.1	32.843	204	111	2	75	252	61	264	5.42e-31	122
MsaG027697	AT5G62000.4	32.843	204	111	2	75	252	61	264	5.46e-31	122
MsaG027697	AT1G59750.2	32.039	206	109	3	75	252	22	224	3.47e-30	120
MsaG027697	AT1G59750.1	32.039	206	109	3	75	252	22	224	3.56e-30	120
MsaG027697	AT1G59750.3	32.039	206	109	3	75	252	22	224	3.56e-30	120
MsaG027697	AT1G59750.4	32.039	206	109	3	75	252	22	224	3.73e-30	119
MsaG027697	AT5G37020.2	34.135	208	102	4	74	250	21	224	5.76e-30	119
MsaG027697	AT5G37020.1	34.135	208	102	4	74	250	21	224	7.69e-30	119
MsaG027697	AT1G35520.1	36.500	200	105	5	75	252	27	226	3.67e-29	116
MsaG027697	AT1G35240.1	36.000	200	106	4	75	252	27	226	4.35e-29	116
MsaG027697	AT1G30330.1	32.618	233	119	5	74	275	22	247	5.89e-29	116
MsaG027697	AT1G30330.3	32.618	233	119	5	74	275	24	249	5.90e-29	116
MsaG027697	AT1G30330.2	32.618	233	119	5	74	275	24	249	5.90e-29	116
MsaG027697	AT1G19850.1	31.356	236	133	4	50	258	31	264	5.98e-28	113
MsaG027697	AT1G19850.2	31.356	236	133	4	50	258	20	253	6.55e-28	113
MsaG027697	AT1G34410.1	35.000	200	108	4	75	252	27	226	2.29e-27	111
MsaG027697	AT5G20730.2	33.493	209	109	5	72	252	21	227	1.87e-26	109
MsaG027697	AT5G20730.1	33.493	209	109	5	72	252	21	227	2.00e-26	109
MsaG027697	AT1G43950.1	36.000	200	99	7	75	250	27	221	2.07e-26	103
MsaG027697	AT5G20730.3	33.493	209	109	5	72	252	21	227	2.07e-26	109
MsaG027697	AT1G19220.1	33.333	213	104	5	72	252	20	226	9.67e-26	107
MsaG027697	AT5G60450.2	30.667	225	115	5	68	254	34	255	8.43e-25	104
MsaG027697	AT5G60450.1	30.769	221	112	5	72	254	62	279	1.61e-24	103
MsaG027697	AT3G61830.2	43.137	102	58	0	151	252	76	177	9.57e-23	98.2
MsaG027697	AT2G46530.2	42.857	105	60	0	148	252	33	137	9.65e-23	98.2
MsaG027697	AT2G33860.2	39.474	114	68	1	152	265	2	114	2.11e-22	96.7
MsaG027697	AT1G34170.3	27.660	235	126	5	53	252	1	226	1.57e-21	94.7
MsaG027697	AT1G34170.2	30.263	228	129	6	53	252	1	226	1.69e-21	94.4
MsaG027697	AT1G34170.1	30.263	228	129	6	53	252	1	226	1.95e-21	94.4
MsaG027697	AT5G37020.3	37.405	131	76	2	122	250	3	129	6.01e-21	93.2

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGAATCTTCGAGATTTC+AGG	0.221871	5:+50940379	None:intergenic
TCGTCTTCGGCAGGAACTTC+TGG	0.250370	5:+50940001	None:intergenic
AGCACATTTCTGCCAGATTT+TGG	0.250965	5:+50940189	None:intergenic
CTGGAGGAGCTTCTTGTTCA+TGG	0.266288	5:+50940020	None:intergenic
AAGTCCATCCCCTACTAAAA+AGG	0.312107	5:+50939765	None:intergenic
TGGCCATGGCCATCCGTTAC+AGG	0.326930	5:+50940040	None:intergenic
TCTAAGCATGTCTTCTCCTT+GGG	0.344156	5:-50940967	None:intergenic
ATCTAAGCATGTCTTCTCCT+TGG	0.347203	5:-50940968	None:intergenic
TGCCAAGGATTGACACAGTT+TGG	0.353376	5:+50940361	None:intergenic
GGTAACAGAGAAGGCGGTTA+TGG	0.361245	5:-50939642	MsaT027697.1:CDS
GGTTATGGATGCGGTGGAAT+TGG	0.365128	5:-50939627	MsaT027697.1:CDS
ACACAATCTGATTGCAATAG+TGG	0.393698	5:-50939941	MsaT027697.1:CDS
GCTCCCCGGTATCACTTAAC+AGG	0.396579	5:+50940257	None:intergenic
CTGACATTCAAGGCCGAGTC+TGG	0.403843	5:-50939826	MsaT027697.1:CDS
GTATATACAGTTCAGCTTTG+TGG	0.420575	5:+50940323	None:intergenic
TTACCATCCCTACCAAAATC+TGG	0.452282	5:-50940201	MsaT027697.1:CDS
GATCCTGCCGAAGCTTAACT+TGG	0.457924	5:-50939882	MsaT027697.1:CDS
TGTATTATCCGAGAGCGAAT+TGG	0.460538	5:-50939578	MsaT027697.1:CDS
CTCTCCGTCACTGACATTCA+AGG	0.461163	5:-50939836	MsaT027697.1:CDS
TCTGTCCTGTTAAGTGATAC+CGG	0.467467	5:-50940262	MsaT027697.1:CDS
TCTGATTGCAATAGTGGCTA+TGG	0.469763	5:-50939935	MsaT027697.1:CDS
GAATCGTCCAAGTTAAGCTT+CGG	0.478925	5:+50939875	None:intergenic
ATTGTAGCCATCCTCGTCTT+CGG	0.480424	5:+50939988	None:intergenic
TAAACTTTAGAATGTAGCTT+CGG	0.481122	5:+50940148	None:intergenic
TTCCAAACTGTGTCAATCCT+TGG	0.497383	5:-50940363	MsaT027697.1:CDS
AAAATCTGGCAGAAATGTGC+TGG	0.507785	5:-50940187	MsaT027697.1:CDS
CTGTCCTGTTAAGTGATACC+GGG	0.511775	5:-50940261	MsaT027697.1:CDS
AAACTGTGTCAATCCTTGGC+AGG	0.512278	5:-50940359	MsaT027697.1:CDS
TAGCCATCCTCGTCTTCGGC+AGG	0.513990	5:+50939992	None:intergenic
AGAGAAGGCGGTTATGGATG+CGG	0.535241	5:-50939636	MsaT027697.1:CDS
GAGCTTCTTGTTCATGGCCA+TGG	0.538260	5:+50940026	None:intergenic
GTTGGAATATGCCGGAAAAG+TGG	0.542178	5:-50939668	MsaT027697.1:CDS
CGTCCAAGTTAAGCTTCGGC+AGG	0.542509	5:+50939879	None:intergenic
AAATCAAACCAATTCGCTCT+CGG	0.544399	5:+50939570	None:intergenic
TTCCATCCTCACTCCTGTAA+CGG	0.546200	5:-50940053	MsaT027697.1:CDS
TATGGATGCGGTGGAATTGG+CGG	0.548189	5:-50939624	MsaT027697.1:CDS
ATCCGTTACAGGAGTGAGGA+TGG	0.551125	5:+50940051	None:intergenic
ACAGAGCTCTCAGCACGTCT+AGG	0.552883	5:+50940280	None:intergenic
AAGGCGGATAACGATGAAGA+AGG	0.557475	5:+50940074	None:intergenic
AGATATTCGTTGGAATATGC+CGG	0.558862	5:-50939676	MsaT027697.1:CDS
TCAGCTGGGAAGATATTCGT+TGG	0.559165	5:-50939686	MsaT027697.1:CDS
AACAACAAGAAACTCGTCGC+TGG	0.566110	5:-50939734	MsaT027697.1:CDS
GGCAGGATCAAGTCAACGCA+AGG	0.570350	5:+50939896	None:intergenic
CATGTTCTAAGTGACCTTGT+GGG	0.576431	5:+50940119	None:intergenic
TTGGAATATGCCGGAAAAGT+GGG	0.577884	5:-50939667	MsaT027697.1:CDS
ACATGTTCTAAGTGACCTTG+TGG	0.589249	5:+50940118	None:intergenic
TGCTTCGGAATGTAGCTCCC+CGG	0.594410	5:+50940243	None:intergenic
TATGCGTGTACTGCCAGACT+CGG	0.597780	5:+50939813	None:intergenic
ATCCTCACTCCTGTAACGGA+TGG	0.600039	5:-50940049	MsaT027697.1:CDS
ACTCCTGTAACGGATGGCCA+TGG	0.603479	5:-50940043	MsaT027697.1:CDS
AGAAGTTCCTGCCGAAGACG+AGG	0.606495	5:-50939999	MsaT027697.1:CDS
TGAATAAGTTCAACCTGCCA+AGG	0.607758	5:+50940346	None:intergenic
GTTACAGGAGTGAGGATGGA+AGG	0.609129	5:+50940055	None:intergenic
AGGCGGATAACGATGAAGAA+GGG	0.617693	5:+50940075	None:intergenic
TCTTCGGCAGGAACTTCTGG+AGG	0.623502	5:+50940004	None:intergenic
AAGGGTGATGATTGTGTGAG+CGG	0.624730	5:+50940093	None:intergenic
AAGTGGGAAGGTAACAGAGA+AGG	0.628618	5:-50939651	MsaT027697.1:CDS
GTTCCTGCCGAAGACGAGGA+TGG	0.629556	5:-50939995	MsaT027697.1:CDS
GAAGGCGGTTATGGATGCGG+TGG	0.634390	5:-50939633	MsaT027697.1:CDS
GGCCATCCGTTACAGGAGTG+AGG	0.635015	5:+50940047	None:intergenic
TTGTGTGAGAATCTTGACAA+AGG	0.644198	5:+50939958	None:intergenic
GTGACGGAGAGTGTCTGAGA+TGG	0.649352	5:+50939848	None:intergenic
AAAGTTTACTACTTCCCACA+AGG	0.649931	5:-50940133	MsaT027697.1:CDS
TCGGCCTTGAATGTCAGTGA+CGG	0.654538	5:+50939832	None:intergenic
GCAGGATCAAGTCAACGCAA+GGG	0.677166	5:+50939897	None:intergenic
TGGGAAGGTAACAGAGAAGG+CGG	0.684452	5:-50939648	MsaT027697.1:CDS
ACAGGAGTGAGGATGGAAGG+CGG	0.699861	5:+50940058	None:intergenic
GGTGTCTCTTGGATTTACCG+CGG	0.707333	5:+50939786	None:intergenic
AATATGCCGGAAAAGTGGGA+AGG	0.715565	5:-50939663	MsaT027697.1:CDS
TGTCCTGTTAAGTGATACCG+GGG	0.717824	5:-50940260	MsaT027697.1:CDS
TAGCTTCGGAATCTTGACTG+AGG	0.723506	5:+50940162	None:intergenic
CAGTACACGCATATCTACCG+CGG	0.778287	5:-50939803	MsaT027697.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	50939565	50940982	50939565	ID=MsaG027697
Chr5	mRNA	50939565	50940982	50939565	ID=MsaT027697.1;Parent=MsaG027697
Chr5	exon	50939565	50940416	50939565	ID=MsaT027697.1.exon2;Parent=MsaT027697.1
Chr5	CDS	50939565	50940416	50939565	ID=cds.MsaT027697.1;Parent=MsaT027697.1
Chr5	exon	50940968	50940982	50940968	ID=MsaT027697.1.exon1;Parent=MsaT027697.1
Chr5	CDS	50940968	50940982	50940968	ID=cds.MsaT027697.1;Parent=MsaT027697.1

Gene Sequence

>MsaT027697.1

ATGTCTTCTCCTTGGGTTAACTGGGATGAACCTGAAATCTCGAAGATTCCAAACTGTGTCAATCCTTGGCAGGTTGAACTTATTCACCACAAAGCTGAACTGTATATACAATCCCCCCCAAATAAAAATCCTAGACGTGCTGAGAGCTCTGTCCTGTTAAGTGATACCGGGGAGCTACATTCCGAAGCATTCCCCACAAAATCTATATTTACCATCCCTACCAAAATCTGGCAGAAATGTGCTGGAGCCTCAGTCAAGATTCCGAAGCTACATTCTAAAGTTTACTACTTCCCACAAGGTCACTTAGAACATGTCTCCGCTCACACAATCATCACCCTTCTTCATCGTTATCCGCCTTCCATCCTCACTCCTGTAACGGATGGCCATGGCCATGAACAAGAAGCTCCTCCAGAAGTTCCTGCCGAAGACGAGGATGGCTACAATGTTGTTTCCTTTGTCAAGATTCTCACACAATCTGATTGCAATAGTGGCTATGGATTCATTGTATCTCTCCCTTGCGTTGACTTGATCCTGCCGAAGCTTAACTTGGACGATTCAATGCCATCTCAGACACTCTCCGTCACTGACATTCAAGGCCGAGTCTGGCAGTACACGCATATCTACCGCGGTAAATCCAAGAGACACCTTTTTAGTAGGGGATGGACTTCATTTGTCAACAACAAGAAACTCGTCGCTGGTGATTCAGTTGTTTTCGTTAAAAACTCAGCTGGGAAGATATTCGTTGGAATATGCCGGAAAAGTGGGAAGGTAACAGAGAAGGCGGTTATGGATGCGGTGGAATTGGCGGTGAAAAAATGGCATTTGAAGTTTTGTATTATCCGAGAGCGAATTGGTTTGATTTTGTAG

Protein sequence

>MsaT027697.1

MSSPWVNWDEPEISKIPNCVNPWQVELIHHKAELYIQSPPNKNPRRAESSVLLSDTGELHSEAFPTKSIFTIPTKIWQKCAGASVKIPKLHSKVYYFPQGHLEHVSAHTIITLLHRYPPSILTPVTDGHGHEQEAPPEVPAEDEDGYNVVSFVKILTQSDCNSGYGFIVSLPCVDLILPKLNLDDSMPSQTLSVTDIQGRVWQYTHIYRGKSKRHLFSRGWTSFVNNKKLVAGDSVVFVKNSAGKIFVGICRKSGKVTEKAVMDAVELAVKKWHLKFCIIRERIGLIL*