AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027702

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027702 MtrunA17_Chr5g0425661 89.593 221 23 0 46 266 8 228 5.36e-145 410
MsaG027702 MtrunA17_Chr5g0424931 57.018 342 99 5 72 374 16 348 2.29e-125 372
MsaG027702 MtrunA17_Chr1g0178011 61.392 316 98 6 75 374 12 319 1.76e-122 362
MsaG027702 MtrunA17_Chr5g0425261 56.782 317 108 8 75 374 12 316 1.22e-108 323
MsaG027702 MtrunA17_Chr5g0424801 54.655 333 119 9 75 390 12 329 1.26e-108 323
MsaG027702 MtrunA17_Chr5g0424741 55.362 345 110 8 72 374 9 351 8.30e-108 327
MsaG027702 MtrunA17_Chr2g0289221 53.355 313 127 9 72 373 17 321 2.98e-96 291
MsaG027702 MtrunA17_Chr5g0417211 51.863 322 135 9 72 381 9 322 1.08e-90 279
MsaG027702 MtrunA17_Chr5g0417281 50.482 311 138 9 72 373 8 311 1.38e-88 277
MsaG027702 MtrunA17_Chr2g0277461 48.598 321 134 10 72 374 10 317 1.97e-81 256
MsaG027702 MtrunA17_Chr5g0436761 48.254 315 145 9 72 374 9 317 2.91e-80 253
MsaG027702 MtrunA17_Chr2g0277531 47.619 315 133 14 75 373 20 318 5.17e-79 246
MsaG027702 MtrunA17_Chr2g0326761 37.950 361 146 8 75 373 11 355 1.01e-68 229
MsaG027702 MtrunA17_Chr3g0115271 38.857 350 155 6 75 373 19 360 2.72e-67 223
MsaG027702 MtrunA17_Chr1g0199681 37.322 351 163 6 74 373 93 437 3.88e-65 219
MsaG027702 MtrunA17_Chr1g0181211 35.574 357 168 6 75 373 27 379 4.35e-61 209
MsaG027702 MtrunA17_Chr7g0264161 33.660 306 180 6 85 373 2 301 3.75e-56 186
MsaG027702 MtrunA17_Chr5g0425651 87.500 88 11 0 286 373 1 88 2.58e-48 160
MsaG027702 MtrunA17_Chr4g0028971 34.153 366 159 10 75 373 12 362 1.42e-47 172
MsaG027702 MtrunA17_Chr7g0264171 28.689 366 192 8 71 373 27 386 6.03e-46 166
MsaG027702 MtrunA17_Chr2g0282961 30.743 296 183 4 71 346 49 342 2.55e-43 159
MsaG027702 MtrunA17_Chr8g0385791 31.250 320 192 7 75 373 59 371 3.59e-42 157
MsaG027702 MtrunA17_Chr2g0276781 32.399 321 188 8 75 374 23 335 1.92e-41 154
MsaG027702 MtrunA17_Chr2g0285461 32.308 325 186 12 74 373 22 337 3.64e-41 155
MsaG027702 MtrunA17_Chr8g0376771 32.298 322 189 11 74 373 22 336 3.66e-41 155
MsaG027702 MtrunA17_Chr5g0433301 32.308 325 186 11 74 373 23 338 3.01e-40 152
MsaG027702 MtrunA17_Chr4g0009751 30.435 322 192 8 75 374 19 330 7.48e-40 149
MsaG027702 MtrunA17_Chr7g0239061 30.650 323 190 10 75 374 14 325 1.66e-39 149
MsaG027702 MtrunA17_Chr3g0109391 32.248 307 177 9 74 358 25 322 2.13e-39 149
MsaG027702 MtrunA17_Chr4g0070181 31.595 326 191 9 71 373 19 335 2.51e-39 149
MsaG027702 MtrunA17_Chr2g0301251 30.084 359 195 11 46 373 2 335 6.61e-39 148
MsaG027702 MtrunA17_Chr8g0346711 31.776 321 188 10 75 374 29 339 8.46e-38 144
MsaG027702 MtrunA17_Chr1g0156321 30.247 324 201 7 71 374 39 357 2.78e-37 143
MsaG027702 MtrunA17_Chr4g0046371 29.355 310 174 5 71 346 34 332 2.60e-36 140
MsaG027702 MtrunA17_Chr4g0029671 31.399 293 174 5 75 341 54 345 7.41e-35 136
MsaG027702 MtrunA17_Chr2g0326281 30.392 306 173 8 75 341 56 360 1.08e-34 135
MsaG027702 MtrunA17_Chr8g0389081 29.846 325 198 8 72 373 29 346 7.36e-34 133
MsaG027702 MtrunA17_Chr5g0424791 45.270 148 70 4 124 271 15 151 9.20e-29 109
MsaG027702 MtrunA17_Chr8g0356211 29.814 161 98 2 189 334 8 168 4.50e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027702 AT1G77850.1 39.886 351 145 7 76 373 20 357 1.35e-73 240
MsaG027702 AT1G77850.2 39.886 351 145 7 76 373 20 357 1.55e-73 240
MsaG027702 AT4G30080.1 35.593 354 164 8 75 373 19 363 1.56e-61 210
MsaG027702 AT2G28350.2 35.580 371 148 10 75 373 10 361 1.29e-57 199
MsaG027702 AT2G28350.1 35.580 371 148 10 75 373 10 361 2.29e-57 199
MsaG027702 AT2G46530.3 31.481 324 187 9 74 374 41 352 3.33e-43 159
MsaG027702 AT2G46530.1 31.481 324 187 9 74 374 20 331 3.52e-43 159
MsaG027702 AT3G61830.1 31.790 324 186 9 74 374 24 335 2.69e-42 156
MsaG027702 AT5G62000.2 30.650 323 192 9 75 374 61 374 3.81e-41 155
MsaG027702 AT5G62000.5 30.650 323 192 9 75 374 61 374 3.81e-41 155
MsaG027702 AT5G62000.3 30.650 323 192 9 75 374 61 374 3.81e-41 155
MsaG027702 AT5G62000.1 30.650 323 192 9 75 374 61 374 3.81e-41 155
MsaG027702 AT5G62000.4 30.650 323 192 9 75 374 61 374 4.14e-41 155
MsaG027702 AT5G37020.2 31.077 325 190 11 74 373 21 336 4.62e-41 154
MsaG027702 AT4G23980.2 31.153 321 191 10 75 374 12 323 7.16e-41 153
MsaG027702 AT4G23980.1 31.153 321 191 10 75 374 12 323 7.81e-41 153
MsaG027702 AT5G37020.1 31.077 325 190 11 74 373 21 336 7.84e-41 154
MsaG027702 AT1G59750.1 30.556 324 190 9 75 374 22 334 1.35e-40 152
MsaG027702 AT1G59750.3 30.556 324 190 9 75 374 22 334 1.35e-40 152
MsaG027702 AT2G33860.1 31.741 293 174 7 75 345 54 342 1.41e-40 152
MsaG027702 AT1G59750.4 30.556 324 190 9 75 374 22 334 1.55e-40 152
MsaG027702 AT1G59750.2 30.556 324 190 9 75 374 22 334 1.65e-40 152
MsaG027702 AT1G30330.1 32.000 325 187 12 74 373 22 337 2.87e-40 152
MsaG027702 AT1G30330.3 32.000 325 187 12 74 373 24 339 3.19e-40 152
MsaG027702 AT1G30330.2 32.000 325 187 12 74 373 24 339 3.19e-40 152
MsaG027702 AT1G34310.1 30.625 320 189 9 75 374 27 333 1.70e-39 149
MsaG027702 AT1G35540.1 30.124 322 188 10 75 374 27 333 4.50e-38 145
MsaG027702 AT1G34390.1 30.960 323 182 11 75 374 27 331 1.01e-37 144
MsaG027702 AT1G35520.1 31.366 322 184 11 75 374 27 333 3.06e-37 142
MsaG027702 AT5G20730.2 31.056 322 192 8 74 373 23 336 1.24e-36 142
MsaG027702 AT1G35240.1 30.435 322 187 10 75 374 27 333 1.30e-36 140
MsaG027702 AT1G19220.1 30.556 324 191 9 74 373 22 335 1.41e-35 139
MsaG027702 AT5G20730.3 30.960 323 192 9 74 373 23 337 4.28e-35 137
MsaG027702 AT5G20730.1 30.960 323 192 9 74 373 23 337 4.64e-35 137
MsaG027702 AT5G60450.2 29.204 339 208 9 68 379 34 367 8.61e-35 136
MsaG027702 AT5G60450.1 29.429 333 203 9 74 379 64 391 9.97e-35 136
MsaG027702 AT1G34410.1 29.503 322 190 10 75 374 27 333 1.21e-34 135
MsaG027702 AT1G19850.1 30.252 357 199 13 50 374 31 369 4.73e-34 134
MsaG027702 AT1G19850.2 30.252 357 199 13 50 374 20 358 4.78e-34 134
MsaG027702 AT1G34170.1 27.507 349 210 11 53 374 1 333 1.06e-31 125
MsaG027702 AT1G43950.1 34.466 206 117 5 75 273 27 221 8.00e-30 115
MsaG027702 AT3G61830.2 31.387 274 159 8 118 374 23 284 8.48e-30 120
MsaG027702 AT1G34170.2 29.181 281 165 8 53 308 1 272 9.84e-30 120
MsaG027702 AT1G34170.3 29.181 281 165 8 53 308 1 272 1.13e-29 120
MsaG027702 AT2G46530.2 32.420 219 126 6 171 374 33 244 2.66e-27 113
MsaG027702 AT2G33860.2 31.892 185 111 3 175 345 2 185 3.84e-27 112
MsaG027702 AT5G37020.3 30.000 250 147 9 143 373 1 241 1.04e-26 112
MsaG027702 ATMG00940.1 46.970 66 35 0 278 343 60 125 2.22e-13 68.2

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTATCCTCCTTCCATTTC+CGG 0.160827 5:-51009179 MsaT027702.1:CDS
TTTGGAATCTTCGAGATTTC+AGG 0.221871 5:+51009494 None:intergenic
GTACATACACTTTCTTCTTT+TGG 0.231423 5:-51008408 MsaT027702.1:CDS
AGCACATTTCTGCCAGATTT+TGG 0.250965 5:+51009304 None:intergenic
CCCTAATTGGCGCATGCTTC+AGG 0.281500 5:-51008430 MsaT027702.1:CDS
CTGAAGGAGCTTCTTGTTCA+TGG 0.313540 5:+51009066 None:intergenic
CTCCTTCTAATGTCCCTAAT+TGG 0.322348 5:-51008443 MsaT027702.1:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.352249 5:+51008429 None:intergenic
TGCCAAGGATTGACACAGTT+TGG 0.353376 5:+51009476 None:intergenic
TCTAAGCATGTCTTCTCCTT+GGG 0.357636 5:-51010084 None:intergenic
ATCTAAGCATGTCTTCTCCT+TGG 0.360176 5:-51010085 None:intergenic
GGTAACAGAGAAGGCGGTTA+TGG 0.361245 5:-51008688 MsaT027702.1:CDS
GGTTATGGATGCGGTGGAAT+TGG 0.365128 5:-51008673 MsaT027702.1:CDS
CCTGAAGCATGCGCCAATTA+GGG 0.377642 5:+51008430 None:intergenic
TGGCCATGGCCATCCGTTAC+GGG 0.387740 5:+51009086 None:intergenic
GCTCCCCGGTATCACTTAAC+AGG 0.396579 5:+51009372 None:intergenic
GACTGAAATGATGCCGGAAA+TGG 0.400752 5:+51009166 None:intergenic
ACACAATCTGATTGCAATAG+TGG 0.402033 5:-51008987 MsaT027702.1:CDS
ATGGCCATGGCCATCCGTTA+CGG 0.402053 5:+51009085 None:intergenic
CTGACATTCAAGGCCGAGTC+TGG 0.403843 5:-51008872 MsaT027702.1:CDS
TACGGGAGTGAGGAGAAGTT+TGG 0.412326 5:+51009103 None:intergenic
GTATATACAGTTCAGCTTTG+TGG 0.420575 5:+51009438 None:intergenic
ATGAAGATTAGTTGGACATC+TGG 0.423644 5:-51008561 MsaT027702.1:CDS
CGCCAATTAGGGACATTAGA+AGG 0.425529 5:+51008441 None:intergenic
AGATGTCCAACTAATCTTCA+TGG 0.435291 5:+51008563 None:intergenic
GATCTTGCCGAAGCTTAACT+TGG 0.446257 5:-51008928 MsaT027702.1:CDS
TGTATTATCCGAGAGCGAAT+TGG 0.460538 5:-51008623 MsaT027702.1:CDS
CTCTCCGTCACTGACATTCA+AGG 0.461163 5:-51008882 MsaT027702.1:CDS
ATGATGCCATGAAGATTAGT+TGG 0.464606 5:-51008569 MsaT027702.1:CDS
TCAGCTGGGAAGATATTCAT+TGG 0.466423 5:-51008732 MsaT027702.1:CDS
TCTGTCCTGTTAAGTGATAC+CGG 0.467467 5:-51009377 MsaT027702.1:CDS
TTACCGTCCCTACCAAAATC+TGG 0.468511 5:-51009316 MsaT027702.1:CDS
GAAGATTCTTCTTTCTTCAA+AGG 0.471685 5:+51008522 None:intergenic
GAATCGTCCAAGTTAAGCTT+CGG 0.478925 5:+51008921 None:intergenic
ATTGTAGCCATCCTCGTCTT+CGG 0.480424 5:+51009034 None:intergenic
TAAACTTTAGAATGTAGCTT+CGG 0.481122 5:+51009263 None:intergenic
AAGTCCATCCCCTACTGAAA+AGG 0.489646 5:+51008811 None:intergenic
TTCCAAACTGTGTCAATCCT+TGG 0.497383 5:-51009478 MsaT027702.1:CDS
AAAATCTGGCAGAAATGTGC+TGG 0.507785 5:-51009302 MsaT027702.1:CDS
CTGTCCTGTTAAGTGATACC+GGG 0.511775 5:-51009376 MsaT027702.1:CDS
AAACTGTGTCAATCCTTGGC+AGG 0.512278 5:-51009474 MsaT027702.1:CDS
TAGCCATCCTCGTCTTCGGC+AGG 0.513990 5:+51009038 None:intergenic
GATGAGATTGTATCAGCCTC+GGG 0.518035 5:-51008490 MsaT027702.1:CDS
AGGAGGATAACGATGAAGAA+GGG 0.521368 5:+51009190 None:intergenic
AAGGAGGATAACGATGAAGA+AGG 0.529163 5:+51009189 None:intergenic
ATTGGAATATGCCGGAAAAG+TGG 0.534162 5:-51008714 MsaT027702.1:CDS
AGAGAAGGCGGTTATGGATG+CGG 0.535241 5:-51008682 MsaT027702.1:CDS
AGATGAGATTGTATCAGCCT+CGG 0.541379 5:-51008491 MsaT027702.1:CDS
AGATATTCATTGGAATATGC+CGG 0.543727 5:-51008722 MsaT027702.1:CDS
AAATCAAACCAATTCGCTCT+CGG 0.544399 5:+51008615 None:intergenic
TATGGATGCGGTGGAATTGG+CGG 0.548189 5:-51008670 MsaT027702.1:CDS
TTGGAATATGCCGGAAAAGT+GGG 0.549503 5:-51008713 MsaT027702.1:CDS
ACAGAGCTCTCAGCACGTCT+AGG 0.552883 5:+51009395 None:intergenic
TCTTCGGCAGGAACCTCTGA+AGG 0.553727 5:+51009050 None:intergenic
ATCAAGTCAACGCAAGGGAG+AGG 0.558883 5:+51008948 None:intergenic
AACAACAAGAAACTCGTCGC+TGG 0.566110 5:-51008780 MsaT027702.1:CDS
GGCAAGATCAAGTCAACGCA+AGG 0.570350 5:+51008942 None:intergenic
TCTGATTGCAATAGTGGCTG+TGG 0.575315 5:-51008981 MsaT027702.1:CDS
CATGTTCTAAGTGACCTTGT+GGG 0.576431 5:+51009234 None:intergenic
GAGCTTCTTGTTCATGGCCA+TGG 0.579918 5:+51009072 None:intergenic
CTCCTCACTCCCGTAACGGA+TGG 0.581441 5:-51009095 MsaT027702.1:CDS
ACATGTTCTAAGTGACCTTG+TGG 0.589249 5:+51009233 None:intergenic
TGCTTCGGAATGTAGCTCCC+CGG 0.594410 5:+51009358 None:intergenic
ACTTCTCCTCACTCCCGTAA+CGG 0.594736 5:-51009099 MsaT027702.1:CDS
TATGCGTGTACTGCCAGACT+CGG 0.597780 5:+51008859 None:intergenic
ACTCCCGTAACGGATGGCCA+TGG 0.599048 5:-51009089 MsaT027702.1:CDS
GGCCATCCGTTACGGGAGTG+AGG 0.606038 5:+51009093 None:intergenic
TGAATAAGTTCAACCTGCCA+AGG 0.607758 5:+51009461 None:intergenic
ATTAACGACTGAAATGATGC+CGG 0.608025 5:+51009160 None:intergenic
AGAGGTTCCTGCCGAAGACG+AGG 0.616143 5:-51009045 MsaT027702.1:CDS
AAGGGTGGTGATTGTGTGAG+CGG 0.624730 5:+51009208 None:intergenic
AGGATAACGATGAAGAAGGG+TGG 0.625769 5:+51009193 None:intergenic
AAGTGGGAAGGTAACAGAGA+AGG 0.628618 5:-51008697 MsaT027702.1:CDS
GTTCCTGCCGAAGACGAGGA+TGG 0.629556 5:-51009041 MsaT027702.1:CDS
GAAGGCGGTTATGGATGCGG+TGG 0.634390 5:-51008679 MsaT027702.1:CDS
GAAATGATGCCGGAAATGGA+AGG 0.642956 5:+51009170 None:intergenic
TTGTGTGAGAATCTTGACAA+AGG 0.644198 5:+51009004 None:intergenic
GTGACGGAGAGTGTCTGAGA+TGG 0.649352 5:+51008894 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.649931 5:-51009248 MsaT027702.1:CDS
TGAACAAGAAGCTCCTTCAG+AGG 0.654011 5:-51009063 MsaT027702.1:CDS
GAAAATACTACATCTGTGTG+AGG 0.654483 5:+51009125 None:intergenic
TCGGCCTTGAATGTCAGTGA+CGG 0.654538 5:+51008878 None:intergenic
GCAAGATCAAGTCAACGCAA+GGG 0.677166 5:+51008943 None:intergenic
TGGGAAGGTAACAGAGAAGG+CGG 0.684452 5:-51008694 MsaT027702.1:CDS
ATGAGATTGTATCAGCCTCG+GGG 0.686243 5:-51008489 MsaT027702.1:CDS
ATGATGCCGGAAATGGAAGG+AGG 0.688005 5:+51009173 None:intergenic
TGAGATTGTATCAGCCTCGG+GGG 0.706802 5:-51008488 MsaT027702.1:CDS
GGTGTCTCTTGCATTTACCG+CGG 0.707333 5:+51008832 None:intergenic
AATATGCCGGAAAAGTGGGA+AGG 0.715565 5:-51008709 MsaT027702.1:CDS
TGTCCTGTTAAGTGATACCG+GGG 0.717824 5:-51009375 MsaT027702.1:CDS
TAGCTTCGGAATCTTGACTG+AGG 0.723506 5:+51009277 None:intergenic
CATCTGTGTGAGGATCAACA+AGG 0.762493 5:+51009135 None:intergenic
CAGTACACGCATATCTACCG+CGG 0.778287 5:-51008849 MsaT027702.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 51008374 51010099 51008374 ID=MsaG027702
Chr5 mRNA 51008374 51010099 51008374 ID=MsaT027702.1;Parent=MsaG027702
Chr5 exon 51008374 51009531 51008374 ID=MsaT027702.1.exon2;Parent=MsaT027702.1
Chr5 CDS 51008374 51009531 51008374 ID=cds.MsaT027702.1;Parent=MsaT027702.1
Chr5 exon 51010085 51010099 51010085 ID=MsaT027702.1.exon1;Parent=MsaT027702.1
Chr5 CDS 51010085 51010099 51010085 ID=cds.MsaT027702.1;Parent=MsaT027702.1
Gene Sequence

>MsaT027702.1

ATGTCTTCTCCTTGGGTTAACTGGGATGAACCTGAAATCTCGAAGATTCCAAACTGTGTCAATCCTTGGCAGGTTGAACTTATTCACCACAAAGCTGAACTGTATATACAATCCCCCCCAAATAAAAATCCTAGACGTGCTGAGAGCTCTGTCCTGTTAAGTGATACCGGGGAGCTACATTCCGAAGCATTCCCCACAAAATCTATATTTACCGTCCCTACCAAAATCTGGCAGAAATGTGCTGGAGCCTCAGTCAAGATTCCGAAGCTACATTCTAAAGTTTACTACTTCCCACAAGGTCACTTAGAACATGTCTCCGCTCACACAATCACCACCCTTCTTCATCGTTATCCTCCTTCCATTTCCGGCATCATTTCAGTCGTTAATCTCCTTGTTGATCCTCACACAGATGTAGTATTTTCCAAACTTCTCCTCACTCCCGTAACGGATGGCCATGGCCATGAACAAGAAGCTCCTTCAGAGGTTCCTGCCGAAGACGAGGATGGCTACAATGTTGTTTCCTTTGTCAAGATTCTCACACAATCTGATTGCAATAGTGGCTGTGGATTCATTGTACCTCTCCCTTGCGTTGACTTGATCTTGCCGAAGCTTAACTTGGACGATTCAATGCCATCTCAGACACTCTCCGTCACTGACATTCAAGGCCGAGTCTGGCAGTACACGCATATCTACCGCGGTAAATGCAAGAGACACCTTTTCAGTAGGGGATGGACTTCATTTGTCAACAACAAGAAACTCGTCGCTGGTGATTCAGTTGTTTTCGTTAAAAACTCAGCTGGGAAGATATTCATTGGAATATGCCGGAAAAGTGGGAAGGTAACAGAGAAGGCGGTTATGGATGCGGTGGAATTGGCGGTGAAAAACATGGCATTTGAAGTTTTGTATTATCCGAGAGCGAATTGGTTTGATTTTGTAGTGGATGCAAATGTTGTTGATGATGCCATGAAGATTAGTTGGACATCTGGAATTCAAGTCAAGCTTCCTTTGAAGAAAGAAGAATCTTCAAATTCAAAGATGAGATTGTATCAGCCTCGGGGGACAATCTCAGATGTTTATAATGCTCCTTCTAATGTCCCTAATTGGCGCATGCTTCAGGTACATACACTTTCTTCTTTTGGTCTTATTATATTTCAAGTGCATAATGTTATCTAG
Protein sequence

>MsaT027702.1

MSSPWVNWDEPEISKIPNCVNPWQVELIHHKAELYIQSPPNKNPRRAESSVLLSDTGELHSEAFPTKSIFTVPTKIWQKCAGASVKIPKLHSKVYYFPQGHLEHVSAHTITTLLHRYPPSISGIISVVNLLVDPHTDVVFSKLLLTPVTDGHGHEQEAPSEVPAEDEDGYNVVSFVKILTQSDCNSGCGFIVPLPCVDLILPKLNLDDSMPSQTLSVTDIQGRVWQYTHIYRGKCKRHLFSRGWTSFVNNKKLVAGDSVVFVKNSAGKIFIGICRKSGKVTEKAVMDAVELAVKNMAFEVLYYPRANWFDFVVDANVVDDAMKISWTSGIQVKLPLKKEESSNSKMRLYQPRGTISDVYNAPSNVPNWRMLQVHTLSSFGLIIFQVHNVI*