AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027739


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027739 MtrunA17_Chr4g0053341 94.397 232 8 1 1 227 1 232 8.14e-165 454
MsaG027739 MtrunA17_Chr8g0352011 67.788 208 47 2 33 220 110 317 6.36e-99 291
MsaG027739 MtrunA17_Chr1g0173841 62.857 210 71 3 13 220 162 366 3.81e-94 281
MsaG027739 MtrunA17_Chr5g0422571 65.672 201 61 3 30 224 59 257 6.84e-94 276
MsaG027739 MtrunA17_Chr2g0321291 57.028 249 80 5 1 225 44 289 8.56e-94 277
MsaG027739 MtrunA17_Chr7g0258011 58.333 240 71 2 5 220 93 327 9.22e-94 278
MsaG027739 MtrunA17_Chr2g0328921 66.337 202 54 3 33 220 88 289 1.31e-91 271
MsaG027739 MtrunA17_Chr4g0026521 56.769 229 87 2 6 222 43 271 1.94e-88 263
MsaG027739 MtrunA17_Chr1g0198651 67.816 174 51 2 56 224 87 260 3.88e-85 254
MsaG027739 MtrunA17_Chr1g0200421 37.557 221 111 9 17 219 181 392 3.29e-31 119
MsaG027739 MtrunA17_Chr2g0320341 37.255 153 87 5 75 220 238 388 1.53e-24 100
MsaG027739 MtrunA17_Chr8g0382121 38.411 151 85 6 74 219 118 265 4.31e-21 89.4
MsaG027739 MtrunA17_Chr7g0263941 35.762 151 87 5 64 213 45 186 8.48e-21 89.7
MsaG027739 MtrunA17_Chr1g0181381 33.113 151 92 4 64 213 49 191 1.60e-20 89.4
MsaG027739 MtrunA17_Chr4g0029521 33.775 151 91 4 64 213 49 191 4.62e-20 88.2
MsaG027739 MtrunA17_Chr2g0326381 35.099 151 89 4 64 213 43 185 1.08e-19 86.7
MsaG027739 MtrunA17_Chr1g0185121 34.194 155 91 5 61 213 48 193 1.38e-19 86.7
MsaG027739 MtrunA17_Chr1g0210821 33.775 151 90 4 64 213 55 196 2.34e-19 86.3
MsaG027739 MtrunA17_Chr1g0162971 34.437 151 89 4 64 213 52 193 2.92e-19 85.5
MsaG027739 MtrunA17_Chr1g0163001 33.775 151 90 4 64 213 54 195 3.14e-19 85.9
MsaG027739 MtrunA17_Chr2g0324991 35.374 147 85 5 64 208 3 141 3.75e-19 80.9
MsaG027739 MtrunA17_Chr1g0199101 34.868 152 87 5 64 213 58 199 4.33e-19 85.5
MsaG027739 MtrunA17_Chr8g0342421 32.911 158 95 5 58 213 66 214 6.00e-19 85.1
MsaG027739 MtrunA17_Chr1g0151351 32.895 152 91 5 64 213 52 194 1.25e-18 84.0
MsaG027739 MtrunA17_Chr8g0373541 33.113 151 91 4 64 213 44 185 1.79e-18 83.6
MsaG027739 MtrunA17_Chr4g0040571 33.544 158 94 5 58 213 43 191 3.36e-18 82.8
MsaG027739 MtrunA17_Chr1g0191991 33.544 158 94 5 58 213 67 215 4.80e-18 82.4
MsaG027739 MtrunA17_Chr2g0330171 32.911 158 95 5 58 213 50 198 5.63e-18 82.0
MsaG027739 MtrunA17_Chr7g0268961 32.895 152 84 6 70 213 33 174 9.48e-18 81.3
MsaG027739 MtrunA17_Chr3g0123941 36.000 150 87 7 74 219 130 274 9.46e-17 77.8
MsaG027739 MtrunA17_Chr3g0141431 31.081 148 92 4 64 209 33 172 5.62e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027739 AT1G34790.1 66.010 203 54 2 35 222 83 285 3.30e-96 283
MsaG027739 AT3G57670.1 69.832 179 54 0 42 220 177 355 3.38e-91 273
MsaG027739 AT1G51220.1 76.250 160 38 0 67 226 163 322 4.08e-90 269
MsaG027739 AT3G20880.1 58.636 220 56 2 34 219 175 393 7.50e-89 268
MsaG027739 AT1G13290.1 65.128 195 58 4 35 223 54 244 2.72e-87 260
MsaG027739 AT1G08290.1 53.600 250 77 5 6 219 72 318 1.14e-85 258
MsaG027739 AT1G34370.3 41.667 168 87 6 61 219 12 177 2.07e-30 114
MsaG027739 AT1G34370.2 37.500 200 114 6 29 219 190 387 7.66e-30 116
MsaG027739 AT1G34370.1 37.500 200 114 6 29 219 190 387 7.66e-30 116
MsaG027739 AT5G22890.1 37.580 157 89 5 75 224 211 365 1.66e-24 100
MsaG027739 AT5G03150.1 34.437 151 90 4 64 213 64 206 4.41e-21 91.3
MsaG027739 AT5G44160.1 34.810 158 92 5 58 213 41 189 8.10e-20 87.8
MsaG027739 AT1G14580.1 31.863 204 122 6 11 213 18 205 9.67e-20 87.4
MsaG027739 AT1G14580.2 31.863 204 122 6 11 213 18 205 9.67e-20 87.4
MsaG027739 AT1G14580.3 31.863 204 122 6 11 213 32 219 1.02e-19 87.4
MsaG027739 AT5G44160.2 34.437 151 89 4 64 213 9 150 1.22e-19 87.0
MsaG027739 AT2G02080.1 33.158 190 106 7 29 213 33 206 1.29e-19 87.4
MsaG027739 AT2G02080.4 33.158 190 106 7 29 213 33 206 1.29e-19 87.4
MsaG027739 AT2G02080.5 33.158 190 106 7 29 213 33 206 1.29e-19 87.4
MsaG027739 AT2G02080.3 33.158 190 106 7 29 213 33 206 1.29e-19 87.4
MsaG027739 AT5G66730.1 33.553 152 89 5 64 213 43 184 2.34e-19 86.7
MsaG027739 AT5G60470.3 33.929 168 100 5 44 209 32 190 2.52e-19 86.3
MsaG027739 AT5G60470.2 33.929 168 100 5 44 209 36 194 2.73e-19 86.3
MsaG027739 AT1G55110.3 33.548 155 92 5 61 213 71 216 4.01e-19 85.5
MsaG027739 AT1G55110.1 33.548 155 92 5 61 213 71 216 4.01e-19 85.5
MsaG027739 AT1G55110.2 33.548 155 92 5 61 213 71 216 4.01e-19 85.5
MsaG027739 AT5G60470.1 34.694 147 88 3 64 209 46 185 4.02e-19 85.5
MsaG027739 AT2G02070.2 34.932 146 85 4 69 213 68 204 5.27e-19 85.5
MsaG027739 AT2G02070.1 34.932 146 85 4 69 213 68 204 5.27e-19 85.5
MsaG027739 AT3G45260.2 35.443 158 92 5 58 213 43 192 7.82e-19 84.7
MsaG027739 AT3G45260.1 35.443 158 92 5 58 213 43 192 7.82e-19 84.7
MsaG027739 AT1G03840.1 33.544 158 94 5 58 213 45 193 1.10e-18 84.7
MsaG027739 AT1G03840.2 33.121 157 94 5 58 213 45 191 1.45e-18 84.3
MsaG027739 AT2G02080.6 36.842 133 74 4 82 213 6 129 2.82e-18 83.2
MsaG027739 AT2G02080.2 36.842 133 74 4 82 213 6 129 2.82e-18 83.2
MsaG027739 AT3G50700.1 30.939 181 112 6 37 213 15 186 8.88e-18 81.6
MsaG027739 AT4G02670.2 32.468 154 95 4 61 213 63 208 1.04e-17 81.3
MsaG027739 AT4G02670.1 32.468 154 95 4 61 213 61 206 1.17e-17 81.3
MsaG027739 AT3G13810.5 31.818 154 96 4 61 213 73 218 2.34e-17 80.9
MsaG027739 AT3G13810.4 31.818 154 96 4 61 213 78 223 2.51e-17 80.5
MsaG027739 AT3G13810.1 31.818 154 96 4 61 213 78 223 2.51e-17 80.5
MsaG027739 AT3G13810.6 31.818 154 96 4 61 213 59 204 2.69e-17 80.5
MsaG027739 AT3G13810.2 31.788 151 94 4 64 213 82 224 3.41e-17 80.1
MsaG027739 AT3G13810.3 31.788 151 94 4 64 213 68 210 3.45e-17 80.1

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCATCTGGTGGGATTTA+AGG 0.074753 5:+51858285 MsaT027739.1:CDS
CTAAGCCATTGAAGGATTTC+AGG 0.257197 5:+51858052 MsaT027739.1:CDS
CCACCAGATGAAGAAGAAAA+AGG 0.280585 5:-51858276 None:intergenic
ATCAAGTTCCTAACAATTAC+TGG 0.290004 5:+51857300 MsaT027739.1:CDS
CATATCAAAGCATTTGGATT+TGG 0.298690 5:+51858246 MsaT027739.1:CDS
AGTGAATCACCAAATCAAAA+TGG 0.332679 5:+51857260 MsaT027739.1:CDS
AAGGATCATATCAAAGCATT+TGG 0.345398 5:+51858240 MsaT027739.1:CDS
GGTGATTCACTTGAATTGTT+AGG 0.354362 5:-51857248 None:intergenic
CAAACATAAGAGGTCACTTA+AGG 0.365934 5:+51858221 MsaT027739.1:CDS
GTTGGTATCCAGTAATTGTT+AGG 0.378277 5:-51857308 None:intergenic
TCCTTCAATGGCTTAGCTCT+TGG 0.384210 5:-51858045 None:intergenic
CCATGTTATTGTTGTTCAAA+TGG 0.386271 5:+51858000 MsaT027739.1:CDS
CAATCGTTACAACAACCTTC+AGG 0.392156 5:+51857391 MsaT027739.1:CDS
TAATTGTTAGGAACTTGATT+TGG 0.405327 5:-51857296 None:intergenic
GTTAGGAAGGCCTATTTGTA+AGG 0.411385 5:-51857231 None:intergenic
CCTTTGCTAAGTAGCTTCCT+AGG 0.417085 5:+51857146 MsaT027739.1:CDS
GTCACAGTGGCCTTACAAAT+AGG 0.422585 5:+51857221 MsaT027739.1:CDS
GTTCAGATTTCAAACATAAG+AGG 0.434145 5:+51858211 MsaT027739.1:CDS
ACTCAGGAACAGATTCTCAT+TGG 0.435131 5:+51857329 MsaT027739.1:CDS
GGTCTAGCAATGGTCGTGGA+TGG 0.472830 5:-51857975 None:intergenic
TCGAAAATGTGGGAAGCCAT+TGG 0.482738 5:+51858125 MsaT027739.1:CDS
CAATCACCTTTCACTGCCAA+TGG 0.484406 5:-51858141 None:intergenic
GTCTAGCAATGGTCGTGGAT+GGG 0.506325 5:-51857974 None:intergenic
CATTGGCAGTGAAAGGTGAT+TGG 0.508045 5:+51858142 MsaT027739.1:CDS
TCCAAGAGCTAAGCCATTGA+AGG 0.510873 5:+51858044 MsaT027739.1:CDS
ATGAGAATCTGTTCCTGAGT+TGG 0.522747 5:-51857326 None:intergenic
ACGTTTACTTGTCGAAAATG+TGG 0.532025 5:+51858114 MsaT027739.1:CDS
AGTGTCCTGAAATCCTTCAA+TGG 0.536755 5:-51858057 None:intergenic
AAGCTACTTAGCAAAGGTAG+AGG 0.560479 5:-51857140 None:intergenic
ATTCACTTGAATTGTTAGGA+AGG 0.564830 5:-51857244 None:intergenic
CGTTTACTTGTCGAAAATGT+GGG 0.576502 5:+51858115 MsaT027739.1:CDS
AAGCGTTGGCTTTGTATCTG+TGG 0.585573 5:+51858189 MsaT027739.1:CDS
TTTGGTGATAGATGCATATG+TGG 0.589947 5:+51857905 MsaT027739.1:intron
GGTAGGTCTAGCAATGGTCG+TGG 0.592864 5:-51857979 None:intergenic
TTGAACAACAATAACATGGT+AGG 0.599315 5:-51857996 None:intergenic
CAATTACTGGATACCAACTC+AGG 0.612200 5:+51857313 MsaT027739.1:CDS
CCTAGGAAGCTACTTAGCAA+AGG 0.615377 5:-51857146 None:intergenic
ACACACTACAAGAGAAAGCA+TGG 0.621285 5:+51858084 MsaT027739.1:CDS
CCATTTGAACAACAATAACA+TGG 0.622921 5:-51858000 None:intergenic
TTGGTGATAGATGCATATGT+GGG 0.631361 5:+51857906 MsaT027739.1:intron
GGGAAGCCATTGGCAGTGAA+AGG 0.634540 5:+51858135 MsaT027739.1:CDS
ATCATGTTTCAATCAAGCTG+AGG 0.648432 5:+51857196 MsaT027739.1:CDS
TAACATGGTAGGTCTAGCAA+TGG 0.662387 5:-51857985 None:intergenic
CATGGTTCGCAGTATCGAAG+AGG 0.675110 5:+51857931 MsaT027739.1:CDS
TGGTGATAGATGCATATGTG+GGG 0.723679 5:+51857907 MsaT027739.1:intron
TCAAGCTGAGGATGTCACAG+TGG 0.732110 5:+51857208 MsaT027739.1:CDS
GGTGATAGATGCATATGTGG+GGG 0.769392 5:+51857908 MsaT027739.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 51857119 51858305 51857119 ID=MsaG027739
Chr5 mRNA 51857119 51858305 51857119 ID=MsaT027739.1;Parent=MsaG027739
Chr5 exon 51857119 51857412 51857119 ID=MsaT027739.1.exon1;Parent=MsaT027739.1
Chr5 CDS 51857119 51857412 51857119 ID=cds.MsaT027739.1;Parent=MsaT027739.1
Chr5 exon 51857916 51858305 51857916 ID=MsaT027739.1.exon2;Parent=MsaT027739.1
Chr5 CDS 51857916 51858305 51857916 ID=cds.MsaT027739.1;Parent=MsaT027739.1
Gene Sequence

>MsaT027739.1

ATGGATCAAAACGATCTACAACCTCTACCTTTGCTAAGTAGCTTCCTAGGTTCAACTCCACAAAATGAAAGTCATAAATCATGTTTCAATCAAGCTGAGGATGTCACAGTGGCCTTACAAATAGGCCTTCCTAACAATTCAAGTGAATCACCAAATCAAAATGGATTTGTAAATGTACCAAATCAAGTTCCTAACAATTACTGGATACCAACTCAGGAACAGATTCTCATTGGATTCAGTCATTTCTCTTGCCCAGTCTGCCTCAAAACTTTCAATCGTTACAACAACCTTCAGATGCATATGTGGGGGCATGGTTCGCAGTATCGAAGAGGACCAGACTCGCTAAAAAGAACCCATCCACGACCATTGCTAGACCTACCATGTTATTGTTGTTCAAATGGATGTAAGAACAACATTGAGCATCCAAGAGCTAAGCCATTGAAGGATTTCAGGACACTGCAGACACACTACAAGAGAAAGCATGGTTCGAAGACGTTTACTTGTCGAAAATGTGGGAAGCCATTGGCAGTGAAAGGTGATTGGAGAACTCATGAGAAAAACTGTGGCAAGCGTTGGCTTTGTATCTGTGGTTCAGATTTCAAACATAAGAGGTCACTTAAGGATCATATCAAAGCATTTGGATTTGGTCATAGCCCTTTTTCTTCTTCATCTGGTGGGATTTAA

Protein sequence

>MsaT027739.1

MDQNDLQPLPLLSSFLGSTPQNESHKSCFNQAEDVTVALQIGLPNNSSESPNQNGFVNVPNQVPNNYWIPTQEQILIGFSHFSCPVCLKTFNRYNNLQMHMWGHGSQYRRGPDSLKRTHPRPLLDLPCYCCSNGCKNNIEHPRAKPLKDFRTLQTHYKRKHGSKTFTCRKCGKPLAVKGDWRTHEKNCGKRWLCICGSDFKHKRSLKDHIKAFGFGHSPFSSSSGGI*