AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027783

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027783 MtrunA17_Chr5g0426961 91.103 843 60 6 1 834 1 837 0.0 1514
MsaG027783 MtrunA17_Chr1g0211711 72.286 783 190 7 68 833 280 1052 0.0 1158
MsaG027783 MtrunA17_Chr1g0183311 63.199 894 255 10 3 833 1 883 0.0 1074
MsaG027783 MtrunA17_Chr1g0211661 69.585 651 191 2 128 772 216 865 0.0 937
MsaG027783 MtrunA17_Chr1g0211691 70.894 615 153 7 129 720 334 945 0.0 874
MsaG027783 MtrunA17_Chr5g0420361 63.902 651 192 8 208 822 145 788 0.0 829
MsaG027783 MtrunA17_Chr6g0472441 60.398 654 190 11 208 822 87 710 0.0 759
MsaG027783 MtrunA17_Chr2g0289121 45.455 814 380 17 33 829 53 819 0.0 624
MsaG027783 MtrunA17_Chr5g0448301 60.646 526 180 5 224 742 126 631 0.0 619
MsaG027783 MtrunA17_Chr4g0075261 45.174 746 321 13 130 829 161 864 0.0 616
MsaG027783 MtrunA17_Chr7g0275771 39.790 857 387 20 87 830 85 925 0.0 556
MsaG027783 MtrunA17_Chr1g0192011 36.437 741 378 13 137 830 267 961 1.39e-150 467
MsaG027783 MtrunA17_Chr2g0289031 58.247 388 158 2 444 829 8 393 1.39e-149 444
MsaG027783 MtrunA17_Chr1g0147021 44.712 539 255 12 131 662 864 1366 1.34e-129 425
MsaG027783 MtrunA17_Chr1g0147021 73.529 136 36 0 685 820 1551 1686 6.72e-58 216
MsaG027783 MtrunA17_Chr2g0281051 33.333 525 326 10 137 652 207 716 9.07e-81 280
MsaG027783 MtrunA17_Chr2g0281051 49.664 149 75 0 685 833 810 958 3.16e-40 160
MsaG027783 MtrunA17_Chr8g0364421 45.736 258 109 3 246 503 3 229 2.10e-64 215
MsaG027783 MtrunA17_Chr1g0211701 83.478 115 19 0 718 832 4 118 3.56e-63 209
MsaG027783 MtrunA17_Chr7g0231061 62.424 165 62 0 505 669 12 176 3.46e-61 205
MsaG027783 MtrunA17_Chr4g0048531 39.057 297 170 5 360 652 727 1016 3.36e-59 219
MsaG027783 MtrunA17_Chr4g0048531 52.703 148 66 2 684 828 1132 1278 1.24e-42 168
MsaG027783 MtrunA17_Chr4g0048531 35.025 197 120 4 109 298 230 425 1.64e-29 126
MsaG027783 MtrunA17_Chr8g0361381 52.406 187 85 1 440 626 26 208 1.87e-58 198
MsaG027783 MtrunA17_Chr2g0289091 61.905 147 46 3 658 794 274 420 3.25e-50 182
MsaG027783 MtrunA17_Chr2g0289091 55.862 145 63 1 515 659 20 163 6.78e-45 167
MsaG027783 MtrunA17_Chr2g0289041 40.191 209 116 4 159 367 2 201 4.76e-44 158
MsaG027783 MtrunA17_Chr8g0361391 73.034 89 23 1 684 772 50 137 3.08e-40 145
MsaG027783 MtrunA17_Chr8g0340381 65.414 133 27 3 539 671 36 149 4.67e-39 143
MsaG027783 MtrunA17_Chr8g0364411 53.982 113 51 1 137 249 33 144 1.24e-32 123
MsaG027783 MtrunA17_Chr1g0211671 77.419 62 14 0 771 832 15 76 2.91e-27 107
MsaG027783 MtrunA17_Chr3g0119761 63.333 60 22 0 612 671 55 114 1.58e-14 71.2
MsaG027783 MtrunA17_Chr2g0289101 32.558 129 81 3 270 397 8 131 1.00e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027783 AT1G11950.1 49.143 700 333 7 133 829 189 868 0.0 677
MsaG027783 AT1G11950.3 49.143 700 333 7 133 829 189 868 0.0 677
MsaG027783 AT1G62310.1 48.696 690 323 11 136 813 208 878 0.0 649
MsaG027783 AT1G11950.2 46.919 633 313 7 133 762 189 801 0.0 573
MsaG027783 AT3G07610.1 56.442 489 200 3 350 833 415 895 2.57e-180 546
MsaG027783 AT3G07610.1 35.806 310 183 5 29 332 49 348 3.77e-48 185
MsaG027783 AT3G07610.3 54.134 508 204 4 350 833 415 917 2.16e-172 526
MsaG027783 AT3G07610.3 35.806 310 183 5 29 332 49 348 3.50e-48 185
MsaG027783 AT4G00990.2 42.727 550 279 13 137 675 80 604 5.22e-133 415
MsaG027783 AT4G00990.2 63.810 105 33 2 666 770 671 770 6.33e-34 140
MsaG027783 AT4G00990.3 42.727 550 279 13 137 675 80 604 5.54e-133 415
MsaG027783 AT4G00990.3 60.177 113 40 2 666 778 671 778 1.08e-34 142
MsaG027783 AT4G00990.1 42.727 550 279 13 137 675 80 604 1.92e-132 416
MsaG027783 AT4G00990.1 61.350 163 58 2 666 828 671 828 1.79e-56 209
MsaG027783 AT1G09060.2 32.708 746 406 16 130 830 197 891 7.93e-121 387
MsaG027783 AT1G09060.1 32.708 746 406 16 130 830 197 891 7.93e-121 387
MsaG027783 AT1G09060.4 32.708 746 406 16 130 830 211 905 1.38e-120 387
MsaG027783 AT1G09060.3 32.708 746 406 16 130 830 211 905 1.38e-120 387
MsaG027783 AT4G21430.1 30.108 744 431 12 131 833 221 916 2.40e-102 338
MsaG027783 AT3G07610.2 55.124 283 120 2 350 632 415 690 1.79e-89 300
MsaG027783 AT3G07610.2 35.806 310 183 5 29 332 49 348 9.90e-49 185

Find 191 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCACCATGCAGATAATT+TGG 0.149377 5:-52559277 None:intergenic
GGATTTGTATATTCTTTAAA+TGG 0.213495 5:-52560265 None:intergenic
AGTACATGCGCTCATCATTA+TGG 0.215750 5:-52559023 None:intergenic
GATCTTCATCACGATGATTT+AGG 0.227135 5:+52559667 MsaT027783.1:CDS
CTTCGTTGTGGGCAGCTTCT+AGG 0.236807 5:+52559214 MsaT027783.1:CDS
ATTCCTTAGATGGTGCTCTT+TGG 0.245043 5:+52561168 MsaT027783.1:CDS
TTCCTTAGATGGTGCTCTTT+GGG 0.262549 5:+52561169 MsaT027783.1:CDS
AATGTGCACCAATTCTTTAC+TGG 0.265399 5:+52560118 MsaT027783.1:CDS
TTGACATTGATGACACATTA+AGG 0.270314 5:-52558197 None:intergenic
AGTACATTGCTGACAATTAC+AGG 0.278275 5:-52559721 None:intergenic
GAACAAGAACAAGTCACTTT+TGG 0.279118 5:+52559810 MsaT027783.1:CDS
AGCATCAGGTGCTTTGTATC+TGG 0.288535 5:-52559314 None:intergenic
GTTGGTGGGGATTCCTTAGA+TGG 0.294738 5:+52561158 MsaT027783.1:CDS
GTCCCAAGGATAATCATAAT+TGG 0.295008 5:+52560152 MsaT027783.1:CDS
TCACATTTAATCTCAGGTTT+TGG 0.298428 5:-52559409 None:intergenic
CTTTCTTCAAATAGTTTAGA+AGG 0.302686 5:-52560205 None:intergenic
TACCTCCAATTTGTCCTCTA+TGG 0.305460 5:-52564099 None:intergenic
TCATATTGCTGAAGCAAAAC+TGG 0.320106 5:+52561019 MsaT027783.1:CDS
TCTATGGACCTGTGATTTAC+TGG 0.329135 5:-52564083 None:intergenic
ATTCCTACGTGGTCAAAATC+TGG 0.333625 5:+52559352 MsaT027783.1:CDS
AGCATTCTGTGCAGCTTCTA+TGG 0.339021 5:-52559159 None:intergenic
GGAGCTACATGGTGATATTC+AGG 0.343116 5:+52561103 MsaT027783.1:CDS
ATGCCCGATGGTTAGATAAA+AGG 0.344516 5:-52562592 None:intergenic
ATCCCAAAGAGCACCATCTA+AGG 0.353655 5:-52561171 None:intergenic
GCTCTTGTTTGAATCCTGTT+AGG 0.355578 5:+52559557 MsaT027783.1:CDS
ACTAACTCGGAGATGCATTC+TGG 0.375260 5:-52559466 None:intergenic
CTTGGCTTCTATCTCCTTCT+CGG 0.376051 5:-52558679 None:intergenic
AGAAGCATTTCAGAGAGTTC+AGG 0.389778 5:+52561237 MsaT027783.1:CDS
GTACATGCGCTCATCATTAT+GGG 0.392626 5:-52559022 None:intergenic
ATATCATCTCTATTCCTAAC+AGG 0.396727 5:-52559571 None:intergenic
AAGTATTGAAATTGAAAGAT+TGG 0.403818 5:+52560179 MsaT027783.1:CDS
AGAGGACGTACCTGCATTGC+AGG 0.406514 5:+52561205 MsaT027783.1:CDS
AGGCCAATTATGATTATCCT+TGG 0.408256 5:-52560155 None:intergenic
CAGCTCTTTGCAAAGAATCC+AGG 0.410316 5:+52559614 MsaT027783.1:CDS
TCTTCCACATTTAATACGAT+TGG 0.410475 5:+52558641 MsaT027783.1:CDS
CTTCACCATGCAGATAATTT+GGG 0.410728 5:-52559276 None:intergenic
TCTTTCTAGAATAGACATAA+TGG 0.411099 5:-52557650 None:intergenic
TTCAATGGCACTGGAGCAAA+GGG 0.413215 5:+52559695 MsaT027783.1:CDS
TCAGGCTGGGCACCAGCATC+AGG 0.415198 5:-52559328 None:intergenic
GATAAGAAGAACAACAATAA+AGG 0.415697 5:+52557809 MsaT027783.1:CDS
TTGTGTACCCAGTAAAGAAT+TGG 0.417175 5:-52560126 None:intergenic
AATGTACTTGAATGTACATC+TGG 0.421952 5:+52559736 MsaT027783.1:CDS
TTACCTTCAGATTTCGGACT+TGG 0.425214 5:-52562808 None:intergenic
AAACCACCGGTAAACGTAAC+CGG 0.429322 5:+52557736 MsaT027783.1:CDS
CCGTGATCACATTTAATCTC+AGG 0.429409 5:-52559415 None:intergenic
AAAATAGTTTGTTGGTTGGT+GGG 0.432080 5:+52561144 MsaT027783.1:CDS
AAAACAGACTCCTCACAATA+AGG 0.433520 5:+52557944 MsaT027783.1:CDS
AACAACAACACGTTCACGAC+GGG 0.435577 5:+52557609 MsaT027783.1:CDS
TTTGACCACGTAGGAATCTC+AGG 0.438455 5:-52559346 None:intergenic
AACAAACGCGGGAGCGGTGG+CGG 0.439355 5:+52557680 MsaT027783.1:CDS
AGGCACTTGATTGCTTAGAT+TGG 0.440544 5:+52559839 MsaT027783.1:CDS
TTCAGATATTCCTGCAATGC+AGG 0.441628 5:-52561215 None:intergenic
AACAATAAAGGCTCCCGTCT+TGG 0.441667 5:+52557821 MsaT027783.1:CDS
AAGAGCTTTGAGCATATACT+TGG 0.441941 5:-52558619 None:intergenic
TATGGATTTGCTGAGGAGCT+TGG 0.447060 5:+52560370 MsaT027783.1:CDS
AAGATAAACTCAAAATCAAA+TGG 0.449448 5:-52559247 None:intergenic
AATGTACATCTGGTTTAAGC+TGG 0.451500 5:+52559746 MsaT027783.1:CDS
GGAGCTTGGACGGGGTGATT+CGG 0.453767 5:+52560384 MsaT027783.1:CDS
GACAAAATCAAGTGCAACCT+TGG 0.456526 5:-52564006 None:intergenic
ATTTGCTGAGGAGCTTGGAC+GGG 0.458809 5:+52560375 MsaT027783.1:CDS
TCTGGCTTTAGAACTTCATC+AGG 0.461474 5:-52560322 None:intergenic
GGTTACGTTTACCGGTGGTT+TGG 0.461832 5:-52557734 None:intergenic
TTCTTCTTGTCTACCAAGAC+GGG 0.465663 5:-52557834 None:intergenic
CAGGTCCATAGAGGACAAAT+TGG 0.466017 5:+52564094 MsaT027783.1:CDS
TTCAGATTTCGGACTTGGTG+GGG 0.467992 5:-52562803 None:intergenic
GGCCAATTATGATTATCCTT+GGG 0.468355 5:-52560154 None:intergenic
GCTGAAGCAAAACTGGATAG+TGG 0.471171 5:+52561026 MsaT027783.1:CDS
GCAAAGCTTGTTTGAAGTCA+CGG 0.474647 5:+52558439 MsaT027783.1:CDS
CCTTCAGATTTCGGACTTGG+TGG 0.476898 5:-52562805 None:intergenic
AAACGCGGGAGCGGTGGCGG+AGG 0.477252 5:+52557683 MsaT027783.1:CDS
GTTGTCGTGAGCTTCGTTGT+GGG 0.478934 5:+52559203 MsaT027783.1:CDS
CATGCAGATAATTTGGGCCT+CGG 0.479508 5:-52559270 None:intergenic
GTCAAAGAAATGACAAGGGC+AGG 0.480713 5:+52558215 MsaT027783.1:CDS
ACGATTGGATCAAGAACAAA+TGG 0.480998 5:+52558656 MsaT027783.1:CDS
ACTACAGTCAATTAAAGAAA+AGG 0.482579 5:+52558560 MsaT027783.1:intron
TGCGGAATTCCTCTGTCAAA+CGG 0.487229 5:-52564055 None:intergenic
ACTAACTTTACCTCCTCTAC+AGG 0.489467 5:-52561275 None:intergenic
TGTGATTTACTGGAAGTTTG+CGG 0.491065 5:-52564073 None:intergenic
ATGTGCACCAATTCTTTACT+GGG 0.492244 5:+52560119 MsaT027783.1:CDS
CTTCAGATTTCGGACTTGGT+GGG 0.495361 5:-52562804 None:intergenic
CACTGTTGAAAATAGTTTGT+TGG 0.496892 5:+52561136 MsaT027783.1:CDS
TTTCTTCTTGTCTACCAAGA+CGG 0.504344 5:-52557835 None:intergenic
GTTGTGGGCAGCTTCTAGGT+GGG 0.507656 5:+52559218 MsaT027783.1:CDS
GATTTGCTGAGGAGCTTGGA+CGG 0.507712 5:+52560374 MsaT027783.1:CDS
GGAAACCAACGATCATCATC+AGG 0.509624 5:+52558587 MsaT027783.1:CDS
TGTATGTAGGTATTGAGCCT+TGG 0.510562 5:+52562732 MsaT027783.1:intron
TGTATATTCTTTAAATGGCA+AGG 0.512473 5:-52560260 None:intergenic
CGTTGTGGGCAGCTTCTAGG+TGG 0.513332 5:+52559217 MsaT027783.1:CDS
CTTTGCTTTACATACTAACT+CGG 0.513784 5:-52559479 None:intergenic
TTTCAATGGCACTGGAGCAA+AGG 0.515097 5:+52559694 MsaT027783.1:CDS
GCTCCCTCTTGTCTTGCTCA+AGG 0.519343 5:-52561085 None:intergenic
GAAAATAGTTTGTTGGTTGG+TGG 0.519502 5:+52561143 MsaT027783.1:CDS
TGTTGTCGTGAGCTTCGTTG+TGG 0.523863 5:+52559202 MsaT027783.1:CDS
ACCACGTAGGAATCTCAGGC+TGG 0.525097 5:-52559342 None:intergenic
GGAAGAAGAAGCAGCTTCAT+CGG 0.527580 5:-52557763 None:intergenic
TCTTTCAATTTCAATACTTG+AGG 0.529125 5:-52560175 None:intergenic
CTTGGTGATGGTGATGGTGA+TGG 0.531700 5:+52557876 MsaT027783.1:CDS
AAATAGTTTGTTGGTTGGTG+GGG 0.532716 5:+52561145 MsaT027783.1:CDS
TTCCTACGTGGTCAAAATCT+GGG 0.534093 5:+52559353 MsaT027783.1:CDS
TTTGTCTCTCCTGAAAATGT+TGG 0.534278 5:+52564023 MsaT027783.1:CDS
TCAGATTTCGGACTTGGTGG+GGG 0.534353 5:-52562802 None:intergenic
TCAAGAACAAATGGCCGAGA+AGG 0.538911 5:+52558665 MsaT027783.1:CDS
TGATCCAATCGTATTAAATG+TGG 0.539950 5:-52558645 None:intergenic
GAGATAGAAGCCAAGTTACA+AGG 0.542112 5:+52558687 MsaT027783.1:CDS
AATGCACGCCACATGACAAA+CGG 0.544435 5:-52559772 None:intergenic
GTCAATTAAAGAAAAGGAGG+AGG 0.545970 5:+52558566 MsaT027783.1:intron
TTTGCTGAGGAGCTTGGACG+GGG 0.546841 5:+52560376 MsaT027783.1:CDS
ATAATGATGAGCGCATGTAC+TGG 0.548032 5:+52559025 MsaT027783.1:CDS
AGCTCTTTGCAAAGAATCCA+GGG 0.549815 5:+52559615 MsaT027783.1:CDS
TGTCCATTGCTCTCCTGTAG+AGG 0.552261 5:+52561262 MsaT027783.1:CDS
TTAGATAAAAGGTCTGATCA+TGG 0.552811 5:-52562581 None:intergenic
TCAGATGCACCAAATGCAAA+CGG 0.554343 5:+52558242 MsaT027783.1:CDS
TAAAAGGTCTGATCATGGAT+GGG 0.555220 5:-52562576 None:intergenic
CTGAAGCAAAACTGGATAGT+GGG 0.556295 5:+52561027 MsaT027783.1:CDS
ATAAAAGGTCTGATCATGGA+TGG 0.557609 5:-52562577 None:intergenic
CTCGACCTGATGATGATCGT+TGG 0.562673 5:-52558592 None:intergenic
AGGTTGAGAGCACCTTTGAA+AGG 0.563537 5:-52560286 None:intergenic
ACTCAGAACAAACGCGGGAG+CGG 0.564215 5:+52557674 MsaT027783.1:CDS
AAGGTATCCTCGATTGAAAG+AGG 0.564928 5:+52558371 MsaT027783.1:intron
GTTGAAAATAGTTTGTTGGT+TGG 0.566984 5:+52561140 MsaT027783.1:CDS
GCTGGGAGCCGTTTGTCATG+TGG 0.567136 5:+52559764 MsaT027783.1:CDS
GAATATACAAATCCTTTCAA+AGG 0.567529 5:+52560274 MsaT027783.1:CDS
GCACCTTGAGCAAGACAAGA+GGG 0.570458 5:+52561082 MsaT027783.1:CDS
AAGTTTACCGCGTCATTGTG+CGG 0.570831 5:+52560225 MsaT027783.1:CDS
ATGTACATCTGGTTTAAGCT+GGG 0.572127 5:+52559747 MsaT027783.1:CDS
CGAGTTAGTATGTAAAGCAA+AGG 0.572266 5:+52559480 MsaT027783.1:CDS
CTAGAATAGACATAATGGAT+AGG 0.573963 5:-52557645 None:intergenic
AGATACAAAGCACCTGATGC+TGG 0.574516 5:+52559316 MsaT027783.1:CDS
AGCTTCTGAACAAAAGTCCA+AGG 0.575249 5:-52562749 None:intergenic
TTTCTTTCGAGAAATTGTGT+TGG 0.575306 5:-52557910 None:intergenic
GAACAACAACACCAAACCAC+CGG 0.575999 5:+52557723 MsaT027783.1:CDS
CCTGAGATTAAATGTGATCA+CGG 0.577057 5:+52559415 MsaT027783.1:CDS
CATCAGGCAGCTTCACAGCA+AGG 0.577705 5:-52560306 None:intergenic
TGATGAAGATCTACAGCCCT+AGG 0.579045 5:-52559655 None:intergenic
CGAGGCCCAAATTATCTGCA+TGG 0.583825 5:+52559271 MsaT027783.1:CDS
AATTGTTCCTCTTTCAATCG+AGG 0.584177 5:-52558378 None:intergenic
GACGGGGTGATTCGGTGACT+AGG 0.584547 5:+52560392 MsaT027783.1:CDS
AAGAAGAAGAATCTTGGTGA+TGG 0.584721 5:+52557864 MsaT027783.1:CDS
GTCCATAGAGGACAAATTGG+AGG 0.587047 5:+52564097 MsaT027783.1:CDS
AGTAAATCACAGGTCCATAG+AGG 0.591019 5:+52564085 MsaT027783.1:CDS
CAGAACAAACGCGGGAGCGG+TGG 0.591548 5:+52557677 MsaT027783.1:CDS
CAAGACAAGAGGGAGCTACA+TGG 0.591607 5:+52561092 MsaT027783.1:CDS
GAATAGACATAATGGATAGG+AGG 0.597179 5:-52557642 None:intergenic
AAGAATCTTGGTGATGGTGA+TGG 0.597832 5:+52557870 MsaT027783.1:CDS
TTGCAGTTACCACAGCAGAC+AGG 0.598583 5:-52558414 None:intergenic
GCAAACTTCCAGTAAATCAC+AGG 0.600882 5:+52564075 MsaT027783.1:CDS
TTGTGGGCAGCTTCTAGGTG+GGG 0.602777 5:+52559219 MsaT027783.1:CDS
AAAAGGTCTGATCATGGATG+GGG 0.603357 5:-52562575 None:intergenic
TCTTCAAATAGTTTAGAAGG+AGG 0.604177 5:-52560202 None:intergenic
ACAGTCAATTAAAGAAAAGG+AGG 0.606305 5:+52558563 MsaT027783.1:intron
CAATAAACAGTGGATTGAAG+AGG 0.614571 5:+52558171 MsaT027783.1:intron
CAACAACAACACGTTCACGA+CGG 0.616055 5:+52557608 MsaT027783.1:CDS
TTGACTCTTTCGAACAGCAA+CGG 0.617245 5:-52557784 None:intergenic
GAGGCATGTCCTGTCTGCTG+TGG 0.617423 5:+52558405 MsaT027783.1:CDS
AGCACCTTGAGCAAGACAAG+AGG 0.618569 5:+52561081 MsaT027783.1:CDS
TATTGCTTATGGATTTGCTG+AGG 0.619448 5:+52560363 MsaT027783.1:CDS
GAAAGAGGAACAATTTGCTG+AGG 0.620045 5:+52558386 MsaT027783.1:CDS
TGAAGTTCTAAAGCCAGACA+TGG 0.620406 5:+52560327 MsaT027783.1:CDS
TCAAAGAAATGACAAGGGCA+GGG 0.621822 5:+52558216 MsaT027783.1:CDS
AATGAAACTTCAAGATGCAG+AGG 0.623550 5:+52559516 MsaT027783.1:CDS
CCACGTAGGAATCTCAGGCT+GGG 0.630864 5:-52559341 None:intergenic
CTACATGGTGATATTCAGGA+TGG 0.633510 5:+52561107 MsaT027783.1:CDS
CCATTGCTCTCCTGTAGAGG+AGG 0.634485 5:+52561265 MsaT027783.1:CDS
ACAACAACACGTTCACGACG+GGG 0.637450 5:+52557610 MsaT027783.1:CDS
CCACCAAGTCCGAAATCTGA+AGG 0.638088 5:+52562805 MsaT027783.1:CDS
TTGCTGTTCGAAAGAGTCAA+CGG 0.641060 5:+52557787 MsaT027783.1:CDS
TCAATGTCAAAGAAATGACA+AGG 0.646775 5:+52558210 MsaT027783.1:CDS
CACGACAACAGAGGAGACAA+AGG 0.649807 5:-52559189 None:intergenic
CCTCCTCTACAGGAGAGCAA+TGG 0.652097 5:-52561265 None:intergenic
ATGAACTCCGCACAATGACG+CGG 0.659262 5:-52560232 None:intergenic
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 5:+52564046 MsaT027783.1:CDS
TTTCCGGTTACGTTTACCGG+TGG 0.665565 5:-52557739 None:intergenic
GAAGTTCTAAAGCCAGACAT+GGG 0.665837 5:+52560328 MsaT027783.1:CDS
TACATGCGCTCATCATTATG+GGG 0.667563 5:-52559021 None:intergenic
ATGATATACACAATAACACA+AGG 0.668038 5:+52559587 MsaT027783.1:CDS
ACAATTTCTCGAAAGAAACC+CGG 0.668784 5:+52557915 MsaT027783.1:CDS
TGGGTACACAAATTGTCCCA+AGG 0.669653 5:+52560138 MsaT027783.1:CDS
AACGAAGCTCACGACAACAG+AGG 0.670801 5:-52559198 None:intergenic
GGGTGGCATGCTGATAGTGA+CGG 0.679311 5:+52559373 MsaT027783.1:CDS
CGTATATGCTATGATTGATG+TGG 0.680480 5:+52564536 MsaT027783.1:CDS
AAGAGACTCAGAACAAACGC+GGG 0.691625 5:+52557669 MsaT027783.1:CDS
TGATTGCTTAGATTGGTGCG+AGG 0.693830 5:+52559846 MsaT027783.1:CDS
GATGTTGTGACCTTATTGTG+AGG 0.707489 5:-52557954 None:intergenic
AAAGAGACTCAGAACAAACG+CGG 0.708789 5:+52557668 MsaT027783.1:CDS
CAATGTCAAAGAAATGACAA+GGG 0.710751 5:+52558211 MsaT027783.1:CDS
CTACGTGGTCAAAATCTGGG+TGG 0.719900 5:+52559356 MsaT027783.1:CDS
GCTCTTTGCAAAGAATCCAG+GGG 0.726479 5:+52559616 MsaT027783.1:CDS
CTGCGTCAGACATATCACAA+TGG 0.731463 5:-52560417 None:intergenic
TGATATACACAATAACACAA+GGG 0.738813 5:+52559588 MsaT027783.1:CDS
CCCAGCCTGAGATTCCTACG+TGG 0.744551 5:+52559341 MsaT027783.1:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 5:+52559696 MsaT027783.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 52557582 52564590 52557582 ID=MsaG027783
Chr5 mRNA 52557582 52564590 52557582 ID=MsaT027783.1;Parent=MsaG027783
Chr5 exon 52557582 52557965 52557582 ID=MsaT027783.1.exon1;Parent=MsaT027783.1
Chr5 CDS 52557582 52557965 52557582 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52558181 52558296 52558181 ID=MsaT027783.1.exon2;Parent=MsaT027783.1
Chr5 CDS 52558181 52558296 52558181 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52558374 52558473 52558374 ID=MsaT027783.1.exon3;Parent=MsaT027783.1
Chr5 CDS 52558374 52558473 52558374 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52558567 52558708 52558567 ID=MsaT027783.1.exon4;Parent=MsaT027783.1
Chr5 CDS 52558567 52558708 52558567 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52558983 52559046 52558983 ID=MsaT027783.1.exon5;Parent=MsaT027783.1
Chr5 CDS 52558983 52559046 52558983 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52559129 52559867 52559129 ID=MsaT027783.1.exon6;Parent=MsaT027783.1
Chr5 CDS 52559129 52559867 52559129 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52560109 52560438 52560109 ID=MsaT027783.1.exon7;Parent=MsaT027783.1
Chr5 CDS 52560109 52560438 52560109 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52561005 52561286 52561005 ID=MsaT027783.1.exon8;Parent=MsaT027783.1
Chr5 CDS 52561005 52561286 52561005 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52562567 52562639 52562567 ID=MsaT027783.1.exon9;Parent=MsaT027783.1
Chr5 CDS 52562567 52562639 52562567 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52562741 52562826 52562741 ID=MsaT027783.1.exon10;Parent=MsaT027783.1
Chr5 CDS 52562741 52562826 52562741 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52563999 52564118 52563999 ID=MsaT027783.1.exon11;Parent=MsaT027783.1
Chr5 CDS 52563999 52564118 52563999 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Chr5 exon 52564522 52564590 52564522 ID=MsaT027783.1.exon12;Parent=MsaT027783.1
Chr5 CDS 52564522 52564590 52564522 ID=cds.MsaT027783.1;Parent=MsaT027783.1
Gene Sequence

>MsaT027783.1

ATGGCGTTTGCGAGGGAACAAGAAGACAACAACAACACGTTCACGACGGGGATTAACAAGCCTCCTATCCATTATGTCTATTCTAGAAAGAGACTCAGAACAAACGCGGGAGCGGTGGCGGAGGAGTTAGCTAAGAACGAAGAACAACAACACCAAACCACCGGTAAACGTAACCGGAAAACCGATGAAGCTGCTTCTTCTTCCGTTGCTGTTCGAAAGAGTCAACGGATAAGAAGAACAACAATAAAGGCTCCCGTCTTGGTAGACAAGAAGAAAAAGAAGAAGAAGAAGAATCTTGGTGATGGTGATGGTGATGGTGCTGAGAAAACCAACACAATTTCTCGAAAGAAACCCGGAAAAAGAAAACAGACTCCTCACAATAAGTGGATTGAAGAGGAGTCCTTAATGTGTCATCAATGTCAAAGAAATGACAAGGGCAGGGTTGTCAGATGCACCAAATGCAAACGGAAAAGATATTGTGTAGACTGTATAAAAAATTGGTATCCTCGATTGAAAGAGGAACAATTTGCTGAGGCATGTCCTGTCTGCTGTGGTAACTGCAATTGCAAAGCTTGTTTGAAGTCACGGAAACTTATAGATTCAATTAAAGAAAAGGAGGAGGAAACCAACGATCATCATCAGGTCGAGCGCTCCAAGTATATGCTCAAAGCTCTTCTTCCACATTTAATACGATTGGATCAAGAACAAATGGCCGAGAAGGAGATAGAAGCCAAGTTACAAGGTCTCTCACTCTCTGAGCTAAAAATTAAAAAATCAAATCCCCATAATGATGAGCGCATGTACTGTGACAACTGCAAAACATCAATATTTGACTACCATAGAAGCTGCACAGAATGCTCTTTTGACCTTTGTCTCCTCTGTTGTCGTGAGCTTCGTTGTGGGCAGCTTCTAGGTGGGGCAGATCCATTTGATTTTGAGTTTATCTTCCGAGGCCCAAATTATCTGCATGGTGAAGTTGCGAAGAAAGTAACCAGATACAAAGCACCTGATGCTGGTGCCCAGCCTGAGATTCCTACGTGGTCAAAATCTGGGTGGCATGCTGATAGTGACGGCAATATTCCCTGTCCAAAACCTGAGATTAAATGTGATCACGGTTACCTTGAACTGAAAAGCGTATTTTCGCCAGAATGCATCTCCGAGTTAGTATGTAAAGCAAAGGAACTTGCAGATTCAATGAAACTTCAAGATGCAGAGGTGACTCTTGATAATAGCTGCTCTTGTTTGAATCCTGTTAGGAATAGAGATGATATACACAATAACACAAGGGAAGCAGCTCTTTGCAAAGAATCCAGGGGCAACTTTTTGTACTGTCCTAGGGCTGTAGATCTTCATCACGATGATTTAGGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAGCCGTTTGTCATGTGGCGTGCATTTCGTCAAATAAACAAGAACAAGAACAAGTCACTTTTGGATGTTAAGGCACTTGATTGCTTAGATTGGTGCGAGATAGATATTAATGTGCACCAATTCTTTACTGGGTACACAAATTGTCCCAAGGATAATCATAATTGGCCTCAAGTATTGAAATTGAAAGATTGGCCTCCTTCTAAACTATTTGAAGAAAGTTTACCGCGTCATTGTGCGGAGTTCATATCTTCCTTGCCATTTAAAGAATATACAAATCCTTTCAAAGGTGCTCTCAACCTTGCTGTGAAGCTGCCTGATGAAGTTCTAAAGCCAGACATGGGACCAAAAACATATATTGCTTATGGATTTGCTGAGGAGCTTGGACGGGGTGATTCGGTGACTAGGCTCCATTGTGATATGTCTGACGCAGTAAATGTGTTGACTCATATTGCTGAAGCAAAACTGGATAGTGGGAGTATTGACGCAATTAAGAAATTGAAACAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATATTCAGGATGGAGAGACCACTGTTGAAAATAGTTTGTTGGTTGGTGGGGATTCCTTAGATGGTGCTCTTTGGGATATTTTTCGGAGAGAGGACGTACCTGCATTGCAGGAATATCTGAAGAAGCATTTCAGAGAGTTCAGGCATGTCCATTGCTCTCCTGTAGAGGAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCATCGGGCATAAGAAAAAGCTGAAGGAAGAGTATGGTATTGAGCCTTGGACTTTTGTTCAGAAGCTTGGAGATGCAATTTTCATTCCAGCTGGTTGCCCCCACCAAGTCCGAAATCTGAAGTCATGCACCAAGGTTGCACTTGATTTTGTCTCTCCTGAAAATGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTGAAGAAAATGATCGTATATGCTATGATTGATGTGGTCAAAAAGTTGGAGAAAGCAAGGAGCCAATGA
Protein sequence

>MsaT027783.1

MAFAREQEDNNNTFTTGINKPPIHYVYSRKRLRTNAGAVAEELAKNEEQQHQTTGKRNRKTDEAASSSVAVRKSQRIRRTTIKAPVLVDKKKKKKKKNLGDGDGDGAEKTNTISRKKPGKRKQTPHNKWIEEESLMCHQCQRNDKGRVVRCTKCKRKRYCVDCIKNWYPRLKEEQFAEACPVCCGNCNCKACLKSRKLIDSIKEKEEETNDHHQVERSKYMLKALLPHLIRLDQEQMAEKEIEAKLQGLSLSELKIKKSNPHNDERMYCDNCKTSIFDYHRSCTECSFDLCLLCCRELRCGQLLGGADPFDFEFIFRGPNYLHGEVAKKVTRYKAPDAGAQPEIPTWSKSGWHADSDGNIPCPKPEIKCDHGYLELKSVFSPECISELVCKAKELADSMKLQDAEVTLDNSCSCLNPVRNRDDIHNNTREAALCKESRGNFLYCPRAVDLHHDDLGHFQWHWSKGEPVIVSNVLECTSGLSWEPFVMWRAFRQINKNKNKSLLDVKALDCLDWCEIDINVHQFFTGYTNCPKDNHNWPQVLKLKDWPPSKLFEESLPRHCAEFISSLPFKEYTNPFKGALNLAVKLPDEVLKPDMGPKTYIAYGFAEELGRGDSVTRLHCDMSDAVNVLTHIAEAKLDSGSIDAIKKLKQKHLEQDKRELHGDIQDGETTVENSLLVGGDSLDGALWDIFRREDVPALQEYLKKHFREFRHVHCSPVEEVIHPIHDQTFYLTIGHKKKLKEEYGIEPWTFVQKLGDAIFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSIEDKLEVKKMIVYAMIDVVKKLEKARSQ*