AlfalfaGEDB | MsaG027804

M. sativa ssp. caerulea / MsaG027804

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00170.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027804	XP_003616032.1	79.221	77	16	0	171	247	90	166	8.76e-33	133
MsaG027804	XP_003616032.1	85.965	171	24	0	1	171	67	237	9.29e-95	292

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027804	sp\|O22873\|BZP18_ARATH	60.215	186	51	4	2	171	48	226	4.16e-59	194

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027804	tr\|G7K0T5\|G7K0T5_MEDTR	79.221	77	16	0	171	247	90	166	4.18e-33	133
MsaG027804	tr\|G7K0T5\|G7K0T5_MEDTR	85.965	171	24	0	1	171	67	237	4.44e-95	292

TFs/TRs:

Gene ID	Type	Classification
MsaG027804	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG027803	MsaG027804	0.838812	4.240005e-57	1.594845e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027804	MtrunA17_Chr5g0432701	85.965	171	24	0	1	171	67	237	8.54e-99	292
MsaG027804	MtrunA17_Chr5g0432701	79.221	77	16	0	171	247	90	166	8.06e-37	133
MsaG027804	MtrunA17_Chr3g0107491	60.465	215	48	5	1	180	54	266	1.37e-73	228
MsaG027804	MtrunA17_Chr3g0107491	41.284	109	30	4	171	247	78	184	2.36e-14	71.6
MsaG027804	MtrunA17_Chr3g0128781	61.905	147	49	1	25	171	47	186	2.43e-50	167
MsaG027804	MtrunA17_Chr2g0329551	48.705	193	55	4	23	171	107	299	2.87e-42	149
MsaG027804	MtrunA17_Chr6g0463571	59.231	130	50	3	45	172	102	230	5.19e-37	132
MsaG027804	MtrunA17_Chr4g0036071	70.213	94	28	0	78	171	345	438	1.02e-33	127
MsaG027804	MtrunA17_Chr1g0156051	56.897	116	50	0	78	193	372	487	1.20e-20	90.5
MsaG027804	MtrunA17_Chr1g0202051	45.000	80	44	0	78	157	109	188	4.54e-13	67.4
MsaG027804	MtrunA17_Chr2g0325131	46.250	80	43	0	92	171	190	269	5.44e-13	67.8
MsaG027804	MtrunA17_Chr2g0316551	47.297	74	39	0	92	165	207	280	2.42e-12	65.9
MsaG027804	MtrunA17_Chr4g0040891	45.946	74	40	0	92	165	193	266	9.45e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027804	AT2G40620.1	60.215	186	51	4	2	171	48	226	4.23e-60	194
MsaG027804	AT2G40620.1	41.667	96	33	3	171	247	64	155	3.00e-11	63.2
MsaG027804	AT1G06850.1	55.696	158	55	3	28	171	70	226	7.75e-48	161
MsaG027804	AT2G31370.5	50.267	187	56	5	23	172	94	280	6.66e-43	149
MsaG027804	AT2G31370.2	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT2G31370.4	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT2G31370.1	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT2G31370.7	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT2G31370.3	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT2G31370.6	50.267	187	56	5	23	172	94	280	1.03e-42	149
MsaG027804	AT1G06070.1	47.514	181	63	3	23	171	108	288	6.12e-40	143
MsaG027804	AT1G06850.2	51.111	135	51	3	28	148	70	203	6.19e-35	125
MsaG027804	AT4G38900.2	71.277	94	27	0	78	171	379	472	1.96e-33	127
MsaG027804	AT4G38900.3	71.277	94	27	0	78	171	379	472	1.96e-33	127
MsaG027804	AT1G43700.1	68.041	97	29	1	77	171	176	272	5.94e-33	122
MsaG027804	AT4G38900.1	67.000	100	27	1	78	171	379	478	4.84e-31	120
MsaG027804	AT2G21230.2	65.957	94	32	0	78	171	355	448	1.86e-30	118
MsaG027804	AT2G21230.3	65.957	94	32	0	78	171	355	448	4.34e-30	117
MsaG027804	AT2G21230.4	65.957	94	32	0	78	171	355	448	4.64e-30	117
MsaG027804	AT2G21230.1	65.957	94	32	0	78	171	355	448	4.64e-30	117
MsaG027804	AT3G58120.2	42.857	84	48	0	93	176	202	285	2.21e-13	68.6
MsaG027804	AT2G42380.5	45.000	80	44	0	92	171	185	264	3.12e-13	68.2
MsaG027804	AT2G42380.2	47.297	74	39	0	92	165	185	258	1.00e-12	67.0
MsaG027804	AT3G58120.1	47.945	73	38	0	93	165	202	274	1.16e-12	67.0
MsaG027804	AT2G12900.1	33.696	92	61	0	93	184	116	207	1.75e-11	63.2
MsaG027804	AT2G12940.1	34.409	93	60	1	79	171	135	226	1.77e-11	63.2

Find 70 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGACATCGGCACAATAATT+TGG	0.242899	5:+52757456	MsaT027804.1:CDS
GTCATTGGGTCGAGTTTGTT+TGG	0.270016	5:+52754895	MsaT027804.1:CDS
TTGTTTGGGGAGACTATAGA+TGG	0.281473	5:+52754910	MsaT027804.1:CDS
GTCGTTGGGTTGAGTTCGTT+TGG	0.295731	5:+52757478	MsaT027804.1:CDS
CGACATAATAATTTGGTCAT+TGG	0.303981	5:+52754880	MsaT027804.1:CDS
GAGACATCGACATAATAATT+TGG	0.326453	5:+52754873	MsaT027804.1:CDS
CAAAGCTCAGCCAACTTATC+AGG	0.329404	5:-52754949	None:intergenic
TCATTGGGTCGAGTTTGTTT+GGG	0.335881	5:+52754896	MsaT027804.1:CDS
CAAAGCTCAGCCAACTTATC+AGG	0.343237	5:-52757532	None:intergenic
TCGTTGGGTTGAGTTCGTTT+GGG	0.362752	5:+52757479	MsaT027804.1:CDS
TGGAGATGCTGGAGCAGATC+CGG	0.379190	5:+52754813	MsaT027804.1:CDS
TGGAGATGCTGGAGCAGATC+CGG	0.379190	5:+52757396	MsaT027804.1:CDS
CTGATAAGTTGGCTGAGCTT+TGG	0.398977	5:+52754950	MsaT027804.1:CDS
CTGATAAGTTGGCTGAGCTT+TGG	0.400539	5:+52757533	MsaT027804.1:CDS
TTCGCACATTTAGGATCAAC+AGG	0.413831	5:-52754973	None:intergenic
GAGTTCGTTTGGGGAGACTA+TGG	0.418532	5:+52757489	MsaT027804.1:CDS
GACATAATAATTTGGTCATT+GGG	0.421535	5:+52754881	MsaT027804.1:CDS
GGCACAATAATTTGGTCGTT+GGG	0.432993	5:+52757464	MsaT027804.1:CDS
CGGCACAATAATTTGGTCGT+TGG	0.438010	5:+52757463	MsaT027804.1:CDS
CGAAGGGGCAGAGAAGAATC+AGG	0.454515	5:+52754843	MsaT027804.1:CDS
TGAATGGATCGGACGGGGAG+AGG	0.474920	5:-52754708	None:intergenic
TCTGCCCCTTCGTTTCCTCC+CGG	0.478769	5:-52754832	None:intergenic
CGAAGAGGCAGAGAAGAATC+CGG	0.481169	5:+52757426	MsaT027804.1:CDS
TCTGCCTCTTCGCTTCCTCC+CGG	0.484387	5:-52757415	None:intergenic
AGCTATGTCTCCTGATAAGT+TGG	0.488757	5:+52754939	MsaT027804.1:CDS
AGCTATGCCTCCTGATAAGT+TGG	0.499388	5:+52757522	MsaT027804.1:CDS
ATGGACAGACTTGGAGATGC+TGG	0.503770	5:+52754802	MsaT027804.1:CDS
ATGGACAGACTTGGAGATGC+TGG	0.503770	5:+52757385	MsaT027804.1:CDS
TGCCCAACTGACACTATACC+AGG	0.512274	5:+52756797	MsaT027804.1:CDS
GTTGATGATCTGCCGGTGAA+TGG	0.513134	5:-52754724	None:intergenic
TGCTCATTCAAAAGAGAGAA+AGG	0.514267	5:+52756710	MsaT027804.1:CDS
TTGATCCTAAATGTGCGAAA+AGG	0.514958	5:+52757560	MsaT027804.1:CDS
TACATTGACATGGACAGACT+TGG	0.516336	5:+52754793	MsaT027804.1:CDS
TACATTGACATGGACAGACT+TGG	0.516336	5:+52757376	MsaT027804.1:CDS
TCGTTTGGGGAGACTATGGA+TGG	0.520293	5:+52757493	MsaT027804.1:CDS
GCAGATCCGGGAGGAAACGA+AGG	0.527514	5:+52754826	MsaT027804.1:CDS
TAAGCTCCGTCCGCAAGCAA+TGG	0.531437	5:+52757262	MsaT027804.1:CDS
CAGATCCGGGAGGAAACGAA+GGG	0.534632	5:+52754827	MsaT027804.1:CDS
TTGATCCTAAATGTGCGAAA+AGG	0.537313	5:+52754977	MsaT027804.1:CDS
GGAGATGCTGGAGCAGATCC+GGG	0.539976	5:+52754814	MsaT027804.1:CDS
GGAGATGCTGGAGCAGATCC+GGG	0.539976	5:+52757397	MsaT027804.1:CDS
GTTGCTCCATTGCTTGCGGA+CGG	0.540579	5:-52757268	None:intergenic
TGCCGGTGAATGGATCGGAC+GGG	0.541814	5:-52754714	None:intergenic
AACCTGGTATAGTGTCAGTT+GGG	0.543208	5:-52756799	None:intergenic
TCCCCGTCCGATCCATTCAC+CGG	0.545509	5:+52754712	MsaT027804.1:CDS
GCCGGTGAATGGATCGGACG+GGG	0.546664	5:-52754713	None:intergenic
TGTTTGGGGAGACTATAGAT+GGG	0.548419	5:+52754911	MsaT027804.1:CDS
GAGTTGTAGCATCAGTCTGT+AGG	0.551575	5:-52756770	None:intergenic
CTGCCGGTGAATGGATCGGA+CGG	0.557304	5:-52754715	None:intergenic
AAACCTGGTATAGTGTCAGT+TGG	0.558403	5:-52756800	None:intergenic
AGTTGTAGCATCAGTCTGTA+GGG	0.565606	5:-52756769	None:intergenic
GCTAGCAGTTGATGATCTGC+CGG	0.567306	5:-52754731	None:intergenic
TGCCGATGTCTCAACCTCAC+CGG	0.569694	5:-52757445	None:intergenic
CGTTGGGTTGAGTTCGTTTG+GGG	0.570030	5:+52757480	MsaT027804.1:CDS
CAACAAACACATCTTCATGA+TGG	0.580671	5:+52757287	MsaT027804.1:CDS
ACTGCTAGCCTCGATGAGAT+CGG	0.580821	5:+52754745	MsaT027804.1:CDS
TTTCGCACATTTAGGATCAA+CGG	0.584714	5:-52757557	None:intergenic
CATTGGGTCGAGTTTGTTTG+GGG	0.585199	5:+52754897	MsaT027804.1:CDS
TGATCTGCCGGTGAATGGAT+CGG	0.590158	5:-52754719	None:intergenic
ATCCGGTGAGGTTGAGACAT+CGG	0.592715	5:+52757443	MsaT027804.1:CDS
GTTTGTTGCTCCATTGCTTG+CGG	0.611180	5:-52757272	None:intergenic
AGCTCAGCCAACTTATCAGG+AGG	0.632846	5:-52757529	None:intergenic
AGATCCGGGAGGAAGCGAAG+AGG	0.647845	5:+52757411	MsaT027804.1:CDS
CGTTTGGGGAGACTATGGAT+GGG	0.670001	5:+52757494	MsaT027804.1:CDS
GGGCAGAGAAGAATCAGGTG+AGG	0.681356	5:+52754848	MsaT027804.1:CDS
AGGCAGAGAAGAATCCGGTG+AGG	0.692593	5:+52757431	MsaT027804.1:CDS
CTTCGGATCCGATCTCATCG+AGG	0.697774	5:-52754753	None:intergenic
GATGCTGGAGCAGATCCGGG+AGG	0.709228	5:+52754817	MsaT027804.1:CDS
GATGCTGGAGCAGATCCGGG+AGG	0.709228	5:+52757400	MsaT027804.1:CDS
AGATCCGGGAGGAAACGAAG+GGG	0.720038	5:+52754828	MsaT027804.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	52754703	52757725	52754703	ID=MsaG027804
Chr5	mRNA	52754703	52757725	52754703	ID=MsaT027804.1;Parent=MsaG027804
Chr5	exon	52754703	52754998	52754703	ID=MsaT027804.1.exon1;Parent=MsaT027804.1
Chr5	CDS	52754703	52754998	52754703	ID=cds.MsaT027804.1;Parent=MsaT027804.1
Chr5	exon	52756686	52756818	52756686	ID=MsaT027804.1.exon2;Parent=MsaT027804.1
Chr5	CDS	52756686	52756818	52756686	ID=cds.MsaT027804.1;Parent=MsaT027804.1
Chr5	exon	52757224	52757308	52757224	ID=MsaT027804.1.exon3;Parent=MsaT027804.1
Chr5	CDS	52757224	52757308	52757224	ID=cds.MsaT027804.1;Parent=MsaT027804.1
Chr5	exon	52757359	52757581	52757359	ID=MsaT027804.1.exon4;Parent=MsaT027804.1
Chr5	CDS	52757359	52757581	52757359	ID=cds.MsaT027804.1;Parent=MsaT027804.1
Chr5	exon	52757710	52757725	52757710	ID=MsaT027804.1.exon5;Parent=MsaT027804.1
Chr5	CDS	52757710	52757725	52757710	ID=cds.MsaT027804.1;Parent=MsaT027804.1

Gene Sequence

>MsaT027804.1

ATGGACCTCTCCCCGTCCGATCCATTCACCGGCAGATCATCAACTGCTAGCCTCGATGAGATCGGATCCGAAGATGACTTGTTTTTGACGTACATTGACATGGACAGACTTGGAGATGCTGGAGCAGATCCGGGAGGAAACGAAGGGGCAGAGAAGAATCAGGTGAGGTCGAGACATCGACATAATAATTTGGTCATTGGGTCGAGTTTGTTTGGGGAGACTATAGATGGGAAGAAAGCTATGTCTCCTGATAAGTTGGCTGAGCTTTGGCCTGTTGATCCTAAATGTGCGAAAAGAATACTTGCTAATCGACAATCTGCTGCTCATTCAAAAGAGAGAAAGGCGCATTATATACATGAACTAGAACGCAAAGTTCAGACCCTACAGACTGATGCTACAACTCTTTCTGCCCAACTGACACTATACCAGAGAGACACAACAGGTCTGAATACTGAAAATACAGAGCTTAAGCTCCGTCCGCAAGCAATGGAGCAACAAACACATCTTCATGATGATGACTTGTTTTTGACGTACATTGACATGGACAGACTTGGAGATGCTGGAGCAGATCCGGGAGGAAGCGAAGAGGCAGAGAAGAATCCGGTGAGGTTGAGACATCGGCACAATAATTTGGTCGTTGGGTTGAGTTCGTTTGGGGAGACTATGGATGGGAAGAAAGCTATGCCTCCTGATAAGTTGGCTGAGCTTTGGTCCGTTGATCCTAAATGTGCGAAAAGTTTAGGCAATTTATAA

Protein sequence

>MsaT027804.1

MDLSPSDPFTGRSSTASLDEIGSEDDLFLTYIDMDRLGDAGADPGGNEGAEKNQVRSRHRHNNLVIGSSLFGETIDGKKAMSPDKLAELWPVDPKCAKRILANRQSAAHSKERKAHYIHELERKVQTLQTDATTLSAQLTLYQRDTTGLNTENTELKLRPQAMEQQTHLHDDDLFLTYIDMDRLGDAGADPGGSEEAEKNPVRLRHRHNNLVVGLSSFGETMDGKKAMPPDKLAELWSVDPKCAKSLGNL*