AlfalfaGEDB | MsaG027811

M. sativa ssp. caerulea / MsaG027811

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003700 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006355 GO:0006417 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903311 GO:1903313 GO:1903506 GO:2000112 GO:2000113 GO:2001141
PFAM: PF00642.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027811	RHN40952.1	79.231	390	63	3	293	669	160	544	0.0	608

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027811	sp\|Q9LT81\|C3H39_ARATH	34.296	277	123	7	8	236	68	333	8.98e-44	165

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027811	tr\|A0A396GIL1\|A0A396GIL1_MEDTR	79.231	390	63	3	293	669	160	544	0.0	608

TFs/TRs:

Gene ID	Type	Classification
MsaG027811	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000350	MsaG027811	0.813796	3.681916e-51	6.905581e-49
MsaG000461	MsaG027811	0.819370	2.104326e-52	4.552022e-50
MsaG000910	MsaG027811	0.802499	9.155430e-49	1.310059e-46
MsaG001503	MsaG027811	0.820917	9.343988e-53	2.105580e-50
MsaG003024	MsaG027811	0.805745	1.947566e-49	3.004298e-47
MsaG003706	MsaG027811	0.831030	3.788807e-55	1.129340e-52
MsaG003806	MsaG027811	0.810439	1.971506e-50	3.403126e-48
MsaG004086	MsaG027811	0.802189	1.059704e-48	1.505578e-46
MsaG004404	MsaG027811	0.803367	6.069693e-49	8.860296e-47
MsaG004737	MsaG027811	0.802189	1.059676e-48	1.505540e-46
MsaG005303	MsaG027811	0.815949	1.233271e-51	2.442067e-49
MsaG005665	MsaG027811	0.831628	2.705878e-55	8.207483e-53
MsaG007269	MsaG027811	0.813787	3.699760e-51	6.937441e-49
MsaG008152	MsaG027811	0.802466	9.300129e-49	1.329740e-46
MsaG008317	MsaG027811	0.800814	2.021358e-48	2.783726e-46
MsaG008616	MsaG027811	0.803556	5.548775e-49	8.135432e-47
MsaG009271	MsaG027811	0.821414	7.186511e-53	1.640828e-50
MsaG009273	MsaG027811	0.819785	1.693826e-52	3.704021e-50
MsaG009425	MsaG027811	0.822615	3.797692e-53	8.954106e-51
MsaG010761	MsaG027811	0.805334	2.372990e-49	3.625614e-47
MsaG010788	MsaG027811	0.805190	2.543554e-49	3.873279e-47
MsaG011499	MsaG027811	0.828486	1.567028e-54	4.344270e-52
MsaG011690	MsaG027811	0.807745	7.397285e-50	1.196438e-47
MsaG011845	MsaG027811	0.804990	2.799662e-49	4.243435e-47
MsaG012001	MsaG027811	0.805121	2.628495e-49	3.996172e-47
MsaG014547	MsaG027811	0.801451	1.499627e-48	2.095185e-46
MsaG017334	MsaG027811	0.808479	5.169507e-50	8.510258e-48
MsaG017559	MsaG027811	0.821570	6.618392e-53	1.517345e-50
MsaG017561	MsaG027811	0.803646	5.317446e-49	7.812242e-47
MsaG017854	MsaG027811	0.815551	1.511571e-51	2.962985e-49
MsaG018785	MsaG027811	0.813280	4.776149e-51	8.842102e-49
MsaG018856	MsaG027811	0.828448	1.599639e-54	4.430049e-52
MsaG020499	MsaG027811	0.813406	4.483135e-51	8.326193e-49
MsaG020940	MsaG027811	0.801588	1.406547e-48	1.971178e-46
MsaG021433	MsaG027811	0.817562	5.381437e-52	1.110638e-49
MsaG022363	MsaG027811	0.820381	1.238867e-52	2.752410e-50
MsaG023747	MsaG027811	0.806905	1.112215e-49	1.763282e-47
MsaG023913	MsaG027811	0.806409	1.413741e-49	2.215397e-47
MsaG027225	MsaG027811	0.825546	7.850858e-54	2.004817e-51
MsaG027811	MsaG027911	0.806645	1.261462e-49	1.987753e-47
MsaG027811	MsaG028158	0.820732	1.030237e-52	2.310063e-50
MsaG027811	MsaG029895	0.814872	2.135488e-51	4.114809e-49
MsaG027811	MsaG030031	0.829597	8.455131e-55	2.418992e-52
MsaG027811	MsaG030094	0.804740	3.155303e-49	4.754815e-47
MsaG027811	MsaG030665	0.805275	2.441396e-49	3.724973e-47
MsaG027811	MsaG031763	0.810509	1.904301e-50	3.292636e-48
MsaG027811	MsaG032089	0.810118	2.310233e-50	3.956571e-48
MsaG027811	MsaG032180	0.803078	6.962866e-49	1.009651e-46
MsaG027811	MsaG033289	0.808312	5.609522e-50	9.197375e-48
MsaG027811	MsaG033589	0.801606	1.394698e-48	1.955404e-46
MsaG027811	MsaG034031	0.813614	4.036840e-51	7.536390e-49
MsaG027811	MsaG034372	0.816402	9.778005e-52	1.958648e-49
MsaG027811	MsaG034625	0.800165	2.738005e-48	3.715636e-46
MsaG027811	MsaG035450	0.830652	4.686384e-55	1.381724e-52
MsaG027811	MsaG036481	0.834280	5.969096e-56	1.956831e-53
MsaG027811	MsaG036658	0.826887	3.777141e-54	1.001212e-51
MsaG027811	MsaG037664	0.819370	2.104021e-52	4.551416e-50
MsaG027811	MsaG037666	0.821809	5.829705e-53	1.345057e-50
MsaG027811	MsaG038519	0.821210	8.004870e-53	1.817883e-50
MsaG027811	MsaG038993	0.800591	2.244171e-48	3.075058e-46
MsaG027811	MsaG039593	0.823685	2.143906e-53	5.203492e-51
MsaG027811	MsaG039975	0.816086	1.149638e-51	2.284350e-49
MsaG027811	MsaG040364	0.817400	5.851078e-52	1.202541e-49
MsaG027811	MsaG041581	0.835583	2.814219e-56	9.592335e-54
MsaG027811	MsaG041746	0.804713	3.196322e-49	4.813523e-47
MsaG027811	MsaG042263	0.802250	1.029910e-48	1.465283e-46
MsaG027811	MsaG042729	0.828658	1.424832e-54	3.968934e-52
MsaG027811	MsaG044542	0.806374	1.438461e-49	2.252207e-47
MsaG027811	MsaG044869	0.818926	2.652475e-52	5.671212e-50
MsaG027811	MsaG045413	0.813115	5.189622e-51	9.568268e-49
MsaG027811	MsaG045759	0.820607	1.100160e-52	2.458778e-50
MsaG027811	MsaG045867	0.802699	8.330639e-49	1.197472e-46
MsaG027811	MsaG046432	0.815884	1.275096e-51	2.520661e-49
MsaG027811	MsaG046962	0.813293	4.745424e-51	8.787993e-49
MsaG027811	MsaG002750	0.813261	4.823008e-51	8.924721e-49
MsaG027811	MsaG001347	0.809255	3.533504e-50	5.926534e-48
MsaG027811	MsaG001992	0.810738	1.699777e-50	2.955442e-48
MsaG027811	MsaG001948	0.818860	2.745982e-52	5.860726e-50
MsaG027811	MsaG013381	0.845871	5.825188e-59	2.747030e-56
MsaG027811	MsaG014915	0.803121	6.821338e-49	9.901208e-47
MsaG027811	MsaG011544	0.802651	8.520288e-49	1.223434e-46
MsaG027811	MsaG016523	0.809113	3.788427e-50	6.332728e-48
MsaG027811	MsaG014890	0.801566	1.421098e-48	1.990583e-46
MsaG027811	MsaG036541	0.829112	1.107083e-54	3.123572e-52
MsaG027811	MsaG036115	0.800263	2.615365e-48	3.557099e-46
MsaG027811	MsaG036763	0.801091	1.776058e-48	2.461129e-46
MsaG027811	MsaG037636	0.803138	6.765271e-49	9.823611e-47
MsaG027811	MsaG036344	0.801042	1.817274e-48	2.515490e-46
MsaG027811	MsaG043110	0.812102	8.624393e-51	1.550470e-48
MsaG027811	MsaG044124	0.812284	7.871023e-51	1.421457e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027811	MtrunA17_Chr8g0360661	79.231	390	63	3	293	669	160	544	0.0	608
MsaG027811	MtrunA17_Chr3g0106071	56.966	323	89	7	293	596	174	465	2.47e-104	331
MsaG027811	MtrunA17_Chr3g0098251	79.570	186	29	1	17	202	3	179	1.45e-103	313
MsaG027811	MtrunA17_Chr3g0098331	56.014	291	98	7	293	580	288	551	5.22e-94	306
MsaG027811	MtrunA17_Chr3g0098261	64.623	212	69	4	289	498	272	479	3.98e-82	274
MsaG027811	MtrunA17_Chr3g0098411	56.322	261	87	7	322	580	3	238	6.54e-82	265
MsaG027811	MtrunA17_Chr4g0063401	54.430	237	73	7	3	205	17	252	6.25e-72	233
MsaG027811	MtrunA17_Chr5g0407201	51.931	233	83	7	351	580	1	207	5.16e-63	214
MsaG027811	MtrunA17_Chr8g0360671	76.692	133	14	2	70	200	41	158	1.73e-62	205
MsaG027811	MtrunA17_Chr4g0063361	50.000	226	84	5	3	200	17	241	4.27e-62	207
MsaG027811	MtrunA17_Chr4g0063391	48.372	215	91	7	1	201	1	209	5.70e-53	182
MsaG027811	MtrunA17_Chr4g0063371	40.741	216	74	6	1	201	1	177	2.02e-38	142
MsaG027811	MtrunA17_Chr3g0098351	38.249	217	111	10	295	503	308	509	1.81e-25	111
MsaG027811	MtrunA17_Chr8g0366501	31.034	203	94	3	42	201	86	285	1.06e-23	103
MsaG027811	MtrunA17_Chr3g0098431	36.316	190	108	7	296	480	101	282	4.10e-22	99.4
MsaG027811	MtrunA17_Chr3g0098321	39.698	199	39	6	473	669	2	121	1.32e-20	89.7
MsaG027811	MtrunA17_Chr3g0098171	48.052	77	37	2	352	428	8	81	7.72e-12	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG027811	AT3G19360.1	34.296	277	123	7	8	236	68	333	9.13e-45	165
MsaG027811	AT3G19360.2	34.296	277	123	7	8	236	68	333	9.13e-45	165
MsaG027811	AT1G32120.1	38.839	224	115	7	286	496	270	484	1.76e-36	147
MsaG027811	AT1G51538.1	41.848	184	95	7	295	475	255	429	1.88e-30	127
MsaG027811	AT5G18510.1	40.217	184	102	5	294	475	253	430	1.39e-28	122
MsaG027811	AT1G50830.1	38.919	185	99	6	296	475	279	454	3.30e-28	121
MsaG027811	AT4G16050.1	28.158	380	238	12	289	648	255	619	1.52e-27	119
MsaG027811	AT1G50790.1	34.101	217	137	4	290	502	256	470	3.37e-26	115
MsaG027811	AT1G50820.2	37.356	174	105	3	293	464	255	426	9.16e-25	110
MsaG027811	AT1G50820.1	36.782	174	106	3	293	464	255	426	5.18e-24	107
MsaG027811	AT1G32360.1	26.978	278	140	6	42	261	85	357	8.93e-23	101
MsaG027811	AT1G50750.1	40.659	91	53	1	384	474	260	349	8.24e-15	77.4

Find 167 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGACGAATGGATGCAGTTTC+TGG	0.174701	5:+52853001	MsaT027811.1:CDS
CGGAGATGGTTCTAATAATT+TGG	0.195023	5:+52853178	MsaT027811.1:CDS
TCCACCAAATCTTTCATAAA+TGG	0.207923	5:-52855443	None:intergenic
CCAAACTTGAACCAAGTAAA+AGG	0.211539	5:-52855210	None:intergenic
CCTTTCGAAATAAAGTTTAA+TGG	0.212060	5:+52854745	MsaT027811.1:CDS
CTGCCTGTTTCTTTGTTTGA+TGG	0.234171	5:-52852840	None:intergenic
CAGCAACGGATTTGAGAAAA+TGG	0.243451	5:-52854648	None:intergenic
GGCACACAAATTTCATCATT+TGG	0.274730	5:-52855422	None:intergenic
CATAGAGTTGTTATGCAGTT+TGG	0.284791	5:+52855545	MsaT027811.1:CDS
CTAATAATTTGGAAGGCAAT+AGG	0.289690	5:+52853189	MsaT027811.1:CDS
AAACAGGGCGCTGTCCCTTT+GGG	0.311148	5:+52853273	MsaT027811.1:CDS
CTCGTATTTGTCATAAGTTC+AGG	0.326388	5:+52852877	MsaT027811.1:CDS
CTCTTGTTCATTGGAAATTG+AGG	0.339686	5:-52852768	None:intergenic
ATAGGCTTAGGGATGATTCT+TGG	0.341417	5:+52853117	MsaT027811.1:CDS
CAAACAGGGCGCTGTCCCTT+TGG	0.343335	5:+52853272	MsaT027811.1:CDS
ATTATTGGCCTCTTGTTCAT+TGG	0.344534	5:-52852777	None:intergenic
TAGTTACAATATCCACCTTT+AGG	0.349503	5:-52852912	None:intergenic
CTTTCGAAATAAAGTTTAAT+GGG	0.352984	5:+52854746	MsaT027811.1:CDS
ATGAACTTGCAATTATGTTC+TGG	0.354176	5:-52855772	None:intergenic
ATTCATGTTGACGATCCTTC+TGG	0.358223	5:+52856287	MsaT027811.1:CDS
TATAGTCAGAGTGTTATCTT+TGG	0.359003	5:-52856414	None:intergenic
TGTCCCATGTGGCAGCAAAC+AGG	0.365446	5:+52853257	MsaT027811.1:CDS
AGCTGTGGCACTGTTACTTT+TGG	0.366201	5:+52855833	MsaT027811.1:CDS
GTCTTCTTTATTGATCCACT+TGG	0.372495	5:-52855267	None:intergenic
TGTATTATTGAAGTGGCATT+TGG	0.373142	5:+52855292	MsaT027811.1:CDS
AGGCAGCAAGGGGAACTTAC+TGG	0.380896	5:+52853222	MsaT027811.1:CDS
ATCATTTCACCTTCTGCTCA+TGG	0.381030	5:+52854601	MsaT027811.1:CDS
TCTGAAAACATTGTTGAAGA+TGG	0.393036	5:+52856341	MsaT027811.1:CDS
GTGCCACAGCTAACAAGTTT+AGG	0.402231	5:-52855821	None:intergenic
TGTGCCATTTATGAAAGATT+TGG	0.411286	5:+52855439	MsaT027811.1:CDS
TTTGGAAAATATAGCGATGA+TGG	0.413895	5:+52855851	MsaT027811.1:CDS
TTTGGGGAGGACTGTCACTA+TGG	0.414198	5:+52853290	MsaT027811.1:CDS
AGCTTCCTTCATTTCAATGA+TGG	0.415768	5:-52854575	None:intergenic
TAAGGCAGCTACCACCGAAT+TGG	0.416824	5:+52852955	MsaT027811.1:CDS
TTCAAGTTTGGGTGTGGGAG+AGG	0.423172	5:+52855222	MsaT027811.1:CDS
ATAGTGCAACCAACAAGTTT+AGG	0.423186	5:-52855932	None:intergenic
AAGATATTGTTATGGTGGTC+TGG	0.424026	5:-52852735	None:intergenic
TCTTGTTCATTGGAAATTGA+GGG	0.426338	5:-52852767	None:intergenic
ATTTCGAAAGGCCGTCCCTT+TGG	0.426942	5:-52854733	None:intergenic
ATGAGACTACTGCCATTGCT+TGG	0.429737	5:+52855606	MsaT027811.1:CDS
GTTTCTTTGTTTGATGGAGG+AGG	0.433415	5:-52852834	None:intergenic
TAATAATTTGGAAGGCAATA+GGG	0.433859	5:+52853190	MsaT027811.1:CDS
ACTTGGTTCAAGTTTGGGTG+TGG	0.434281	5:+52855216	MsaT027811.1:CDS
AGGTTTGGTAATTGTCCTAA+AGG	0.435581	5:+52852897	MsaT027811.1:CDS
ATTTGTCATAAGTTCAGGTT+TGG	0.439137	5:+52852882	MsaT027811.1:CDS
TGAACTTGCAATTATGTTCT+GGG	0.441316	5:-52855771	None:intergenic
TATTGTTGATGATGCTGATC+GGG	0.442139	5:+52856256	MsaT027811.1:CDS
AGGCAATAGGGCTGTGATCA+AGG	0.451978	5:+52853202	MsaT027811.1:CDS
GCAACGGTGATAATATTGTT+GGG	0.452565	5:+52855885	MsaT027811.1:CDS
AATGGTGAGGAATGTCCTTA+TGG	0.454214	5:+52853074	MsaT027811.1:CDS
TGGTTGTTCATGAGTATTGT+TGG	0.456376	5:-52854536	None:intergenic
AGGAGGGCATATGAATTCAT+TGG	0.458224	5:-52852817	None:intergenic
CCTCCATCAAACAAAGAAAC+AGG	0.458374	5:+52852837	MsaT027811.1:CDS
TGGTAGATTTCCCTCGGTGT+TGG	0.458620	5:+52855169	MsaT027811.1:intron
AGCAACGGTGATAATATTGT+TGG	0.460343	5:+52855884	MsaT027811.1:CDS
TCGCTTCAAAGATATTGTTA+TGG	0.462515	5:-52852743	None:intergenic
ATAGTCAGAGTGTTATCTTT+GGG	0.462566	5:-52856413	None:intergenic
CAGAAAATCATTCACAAGAT+GGG	0.463529	5:+52853032	MsaT027811.1:CDS
AGATGATGAAGATGGATTGA+AGG	0.464524	5:-52854692	None:intergenic
TTCAAGATGGTATGAAAGCT+GGG	0.466434	5:+52856132	MsaT027811.1:intron
CTCATGGGGTTGGTGAGATA+AGG	0.466573	5:+52852937	MsaT027811.1:CDS
TCAGAAAATCATTCACAAGA+TGG	0.468298	5:+52853031	MsaT027811.1:CDS
TTCAAGATGGTATGAAAGCT+GGG	0.468916	5:+52856207	MsaT027811.1:CDS
TTATCAGCATATTTAACATA+AGG	0.469285	5:-52855383	None:intergenic
CCAGCTACCTCCTTCCAATT+CGG	0.472104	5:-52852969	None:intergenic
TGAGACTACTGCCATTGCTT+GGG	0.476256	5:+52855607	MsaT027811.1:CDS
CCTGTTTCTTTGTTTGATGG+AGG	0.476598	5:-52852837	None:intergenic
GGTTGTTCATGAGTATTGTT+GGG	0.477495	5:-52854535	None:intergenic
GCCATTTATGAAAGATTTGG+TGG	0.479578	5:+52855442	MsaT027811.1:CDS
AAATACCATCATTGAAATGA+AGG	0.479783	5:+52854570	MsaT027811.1:CDS
CACTATGGTCATGCAGAAGC+AGG	0.481354	5:+52853305	MsaT027811.1:CDS
GGAATGGATCAAGATGTTCC+AGG	0.481871	5:+52855566	MsaT027811.1:CDS
TGGTGGAAGAAATCAGTATT+GGG	0.482188	5:+52855719	MsaT027811.1:CDS
AGCAACGGATTTGAGAAAAT+GGG	0.484851	5:-52854647	None:intergenic
GTTCAAGATGGTATGAAAGC+TGG	0.485749	5:+52856131	MsaT027811.1:intron
CTTGGTTCAAGTTTGGGTGT+GGG	0.487218	5:+52855217	MsaT027811.1:CDS
TTTCTTTGTTTGATGGAGGA+GGG	0.488896	5:-52852833	None:intergenic
CAATACTCATGAACAACCAT+TGG	0.489629	5:+52854540	MsaT027811.1:CDS
AAACTACACTTATCACCATA+AGG	0.492600	5:-52853089	None:intergenic
CGCCCTGTTTGCTGCCACAT+GGG	0.493119	5:-52853260	None:intergenic
GTGGTGGAAGAAATCAGTAT+TGG	0.496605	5:+52855718	MsaT027811.1:CDS
TTGAACCTAGCCACATAACC+TGG	0.497872	5:-52855584	None:intergenic
AGTTGTTATGCAGTTTGGAA+TGG	0.500274	5:+52855550	MsaT027811.1:CDS
TAGATTTCCCTCGGTGTTGG+AGG	0.501183	5:+52855172	MsaT027811.1:intron
TTATTGATCCACTTGGGTCG+TGG	0.502978	5:-52855260	None:intergenic
GGGGAAATATTGATGCTGAT+GGG	0.507548	5:+52856153	MsaT027811.1:CDS
ATCGGAAAATCTAGTGATGA+TGG	0.508585	5:+52855959	MsaT027811.1:CDS
GATATTGTAACTATGCTCAT+GGG	0.511020	5:+52852922	MsaT027811.1:CDS
CCATTAAACTTTATTTCGAA+AGG	0.511795	5:-52854745	None:intergenic
GGATATTGTAACTATGCTCA+TGG	0.512690	5:+52852921	MsaT027811.1:CDS
GCTAACAAGTTTAGGAATCT+CGG	0.515369	5:-52855813	None:intergenic
CTGGCCCTCGCAACGACGAA+TGG	0.515528	5:+52852988	MsaT027811.1:CDS
GCGCCCTGTTTGCTGCCACA+TGG	0.515715	5:-52853261	None:intergenic
GGTGAAGAAGATGATGAAGA+TGG	0.518344	5:-52854700	None:intergenic
AAATGGGCATTTCTGAAAGT+AGG	0.520659	5:-52854631	None:intergenic
GATGGTTCTAATAATTTGGA+AGG	0.521303	5:+52853182	MsaT027811.1:CDS
GTCCCATGTGGCAGCAAACA+GGG	0.525337	5:+52853258	MsaT027811.1:CDS
ATGTTCCAGGTTATGTGGCT+AGG	0.527753	5:+52855579	MsaT027811.1:CDS
GGAAATATTGATGCTGATGG+GGG	0.528704	5:+52856155	MsaT027811.1:CDS
CCGAATTGGAAGGAGGTAGC+TGG	0.528816	5:+52852969	MsaT027811.1:CDS
TTGAGAGTTTCAGAGCTAGT+TGG	0.528890	5:+52855494	MsaT027811.1:CDS
CTCAATTTCCAATGAACAAG+AGG	0.531343	5:+52852769	MsaT027811.1:CDS
GCATTAAGCATTGGAAGTAT+CGG	0.533941	5:+52853158	MsaT027811.1:CDS
AAGACCATGTATTATTGAAG+TGG	0.537019	5:+52855285	MsaT027811.1:CDS
TCAAGATGTTCCAGGTTATG+TGG	0.540300	5:+52855574	MsaT027811.1:CDS
GGGAAATATTGATGCTGATG+GGG	0.540612	5:+52856154	MsaT027811.1:CDS
CAAACTTGAACCAAGTAAAA+GGG	0.541158	5:-52855209	None:intergenic
GTTCAAGATGGTATGAAAGC+TGG	0.541575	5:+52856206	MsaT027811.1:CDS
GGCTGTGATCAAGGCAGCAA+GGG	0.543600	5:+52853211	MsaT027811.1:CDS
GAAAATTGACAATGTGAGGT+TGG	0.544999	5:+52855325	MsaT027811.1:CDS
GTTGGTTGCACTATTACCAT+CGG	0.550508	5:+52855941	MsaT027811.1:CDS
TAGTGACAGTCCTCCCCAAA+GGG	0.553368	5:-52853287	None:intergenic
CTTCAAAGATATTGTTATGG+TGG	0.555249	5:-52852740	None:intergenic
CTTTGAAAATTGACAATGTG+AGG	0.555513	5:+52855321	MsaT027811.1:CDS
AACAGGGCGCTGTCCCTTTG+GGG	0.556253	5:+52853274	MsaT027811.1:CDS
GAGGCCTATGCATTAAGCAT+TGG	0.556818	5:+52853149	MsaT027811.1:CDS
ACTTCCAATGCTTAATGCAT+AGG	0.559099	5:-52853153	None:intergenic
ATGAGTGGGACTTGCAGCAA+CGG	0.560016	5:-52854662	None:intergenic
GGGCTGTGATCAAGGCAGCA+AGG	0.562998	5:+52853210	MsaT027811.1:CDS
AAATCAGTATTGGGTCCTCA+AGG	0.563740	5:+52855728	MsaT027811.1:CDS
CACTCTGACTATATGTGTTG+AGG	0.568473	5:+52856424	MsaT027811.1:CDS
TGGTTGTGGTAGATTTCCCT+CGG	0.571102	5:+52855163	MsaT027811.1:intron
TCTTCTTTATTGATCCACTT+GGG	0.574326	5:-52855266	None:intergenic
GCATCCATTCGTCGTTGCGA+GGG	0.574844	5:-52852992	None:intergenic
CGGTGATAATATTGTTGGGG+AGG	0.576705	5:+52855889	MsaT027811.1:CDS
TGCATCCATTCGTCGTTGCG+AGG	0.576815	5:-52852993	None:intergenic
GATGTTCGTGTTGTTCAAGA+TGG	0.576863	5:+52856194	MsaT027811.1:CDS
TTTGGTAATTGTCCTAAAGG+TGG	0.589355	5:+52852900	MsaT027811.1:CDS
AGCATTGGAAGTATCGGAGA+TGG	0.590771	5:+52853164	MsaT027811.1:CDS
TGTAACTATGCTCATGGGGT+TGG	0.592945	5:+52852927	MsaT027811.1:CDS
AACTGCATAACAACTCTATG+TGG	0.600412	5:-52855542	None:intergenic
AGGGCGCTGTCCCTTTGGGG+AGG	0.602360	5:+52853277	MsaT027811.1:CDS
GGTTCAAAACCATGAGCAGA+AGG	0.602450	5:-52854610	None:intergenic
AGAAAATCATTCACAAGATG+GGG	0.602501	5:+52853033	MsaT027811.1:CDS
AGTTTAGGAATCTCGGAATG+AGG	0.602506	5:-52855806	None:intergenic
GTCAGAGTGTTATCTTTGGG+AGG	0.603842	5:-52856410	None:intergenic
ATATTGTTGATGATGCTGAT+CGG	0.613783	5:+52856255	MsaT027811.1:CDS
ATTCCTAAACTTGTTAGCTG+TGG	0.617564	5:+52855818	MsaT027811.1:CDS
GCAGCTACCACCGAATTGGA+AGG	0.620245	5:+52852959	MsaT027811.1:CDS
ATTTACAACCACGACCCAAG+TGG	0.624238	5:+52855252	MsaT027811.1:CDS
AGTTTAGGAATTGAACCAGA+AGG	0.624334	5:-52856302	None:intergenic
CAACGGTGATAATATTGTTG+GGG	0.627697	5:+52855886	MsaT027811.1:CDS
TCAAGATGGTATGAAAGCTG+GGG	0.635665	5:+52856133	MsaT027811.1:intron
GCTACCTCCTTCCAATTCGG+TGG	0.638241	5:-52852966	None:intergenic
AGTTTAGGAATAAAATCAGA+AGG	0.640134	5:-52855917	None:intergenic
AATGCCACTTCAATAATACA+TGG	0.641443	5:-52855289	None:intergenic
TTGAAGGTAAGTTGATGAGT+GGG	0.645880	5:-52854676	None:intergenic
ATAGTGACAGTCCTCCCCAA+AGG	0.646765	5:-52853288	None:intergenic
CCACCACTTTGCATAACGAG+TGG	0.647021	5:-52855702	None:intergenic
ATTGAAGGTAAGTTGATGAG+TGG	0.647356	5:-52854677	None:intergenic
CCACTCGTTATGCAAAGTGG+TGG	0.648276	5:+52855702	MsaT027811.1:CDS
ACATTAACCTCCAACACCGA+GGG	0.657347	5:-52855179	None:intergenic
TCAAGATGGTATGAAAGCTG+GGG	0.657971	5:+52856208	MsaT027811.1:CDS
CAAGAAATACTACAATGGTG+AGG	0.666991	5:+52853061	MsaT027811.1:CDS
GCTGTGATCAAGGCAGCAAG+GGG	0.668444	5:+52853212	MsaT027811.1:CDS
CTATGCAAGAAATACTACAA+TGG	0.669884	5:+52853056	MsaT027811.1:CDS
TCATTCTTAACGAAACCTTG+AGG	0.674116	5:-52855743	None:intergenic
CAAGATGGTATGAAAGCTGG+GGG	0.674333	5:+52856134	MsaT027811.1:intron
CAAGATGGTATGAAAGCTGG+GGG	0.674333	5:+52856209	MsaT027811.1:CDS
AACATTAACCTCCAACACCG+AGG	0.674394	5:-52855180	None:intergenic
TGATTCTTGGAAATCAAGAG+AGG	0.676002	5:+52853130	MsaT027811.1:CDS
GCTACCACCGAATTGGAAGG+AGG	0.676137	5:+52852962	MsaT027811.1:CDS
ATATTGTAACTATGCTCATG+GGG	0.682160	5:+52852923	MsaT027811.1:CDS
TTACCACTCGTTATGCAAAG+TGG	0.689210	5:+52855699	MsaT027811.1:CDS
ATTGTTGATGATGCTGATCG+GGG	0.698629	5:+52856257	MsaT027811.1:CDS
GAACTTGCAATTATGTTCTG+GGG	0.723227	5:-52855770	None:intergenic
AGATTAAGCTGTGTCCCATG+TGG	0.762737	5:+52853246	MsaT027811.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	52852726	52856472	52852726	ID=MsaG027811
Chr5	mRNA	52852726	52856472	52852726	ID=MsaT027811.1;Parent=MsaG027811
Chr5	exon	52852726	52853326	52852726	ID=MsaT027811.1.exon1;Parent=MsaT027811.1
Chr5	CDS	52852726	52853326	52852726	ID=cds.MsaT027811.1;Parent=MsaT027811.1
Chr5	exon	52854506	52854767	52854506	ID=MsaT027811.1.exon2;Parent=MsaT027811.1
Chr5	CDS	52854506	52854767	52854506	ID=cds.MsaT027811.1;Parent=MsaT027811.1
Chr5	exon	52855175	52855998	52855175	ID=MsaT027811.1.exon3;Parent=MsaT027811.1
Chr5	CDS	52855175	52855998	52855175	ID=cds.MsaT027811.1;Parent=MsaT027811.1
Chr5	exon	52856138	52856472	52856138	ID=MsaT027811.1.exon4;Parent=MsaT027811.1
Chr5	CDS	52856138	52856472	52856138	ID=cds.MsaT027811.1;Parent=MsaT027811.1

Gene Sequence

>MsaT027811.1

ATGACTTTTCCAGACCACCATAACAATATCTTTGAAGCGAGCCCTCAATTTCCAATGAACAAGAGGCCAATAATTTCTGAAAACAATCATTCCAATGAATTCATATGCCCTCCTCCATCAAACAAAGAAACAGGCAGCATTTTCTTCAAGACTCGTATTTGTCATAAGTTCAGGTTTGGTAATTGTCCTAAAGGTGGATATTGTAACTATGCTCATGGGGTTGGTGAGATAAGGCAGCTACCACCGAATTGGAAGGAGGTAGCTGGCCCTCGCAACGACGAATGGATGCAGTTTCTGGATGATGATCAGAAAATCATTCACAAGATGGGGCTATGCAAGAAATACTACAATGGTGAGGAATGTCCTTATGGTGATAAGTGTAGTTTTCTTCATAGGCTTAGGGATGATTCTTGGAAATCAAGAGAGGCCTATGCATTAAGCATTGGAAGTATCGGAGATGGTTCTAATAATTTGGAAGGCAATAGGGCTGTGATCAAGGCAGCAAGGGGAACTTACTGGAAGATTAAGCTGTGTCCCATGTGGCAGCAAACAGGGCGCTGTCCCTTTGGGGAGGACTGTCACTATGGTCATGCAGAAGCAGATTATAGCTTTATGATGAATAATCTTAAACCCAACAATACTCATGAACAACCATTGGAAAATCAAAATACCATCATTGAAATGAAGGAAGCTTTCATCATTTCACCTTCTGCTCATGGTTTTGAACCTACTTTCAGAAATGCCCATTTTCTCAAATCCGTTGCTGCAAGTCCCACTCATCAACTTACCTTCAATCCATCTTCATCATCTTCTTCACCTGTTTTTATTCCAAAGGGACGGCCTTTCGAAATAAAGTTTAATGGATTTCCCTCGGTGTTGGAGGTTAATGTTCAATCACCCTTTTACTTGGTTCAAGTTTGGGTGTGGGAGAGGTTCAAAAATTTACAACCACGACCCAAGTGGATCAATAAAGAAGACCATGTATTATTGAAGTGGCATTTGGTTAATGCTTTGAAAATTGACAATGTGAGGTTGGCGTTAGATTCAGCTGTTGATAATTTTATTTGGCGTCCTTATGTTAAATATGCTGATAAGTGTAGACTGTTTTATCCAAATGATGAAATTTGTGTGCCATTTATGAAAGATTTGGTGGATAAACAAATGTTGTCATTTGTTATATGCTTGAGAGTTTCAGAGCTAGTTGGATTTGAGTCTATAGAGAAATATCTACCACATAGAGTTGTTATGCAGTTTGGAATGGATCAAGATGTTCCAGGTTATGTGGCTAGGTTCAATGAGACTACTGCCATTGCTTGGGAAAATTATTGCAGATCTCTTTCTGATACAAGTTTATGTTATTTTCCATCTAGATTTTTTAAGTCTGATGTTACCACTCGTTATGCAAAGTGGTGGAAGAAATCAGTATTGGGTCCTCAAGGTTTCGTTAAGAATGATGTGCCCCAGAACATAATTGCAAGTTCATCAAAATATAGACCTCATTCCGAGATTCCTAAACTTGTTAGCTGTGGCACTGTTACTTTTGGAAAATATAGCGATGATGGTTTAAAAACGAGCAACGGTGATAATATTGTTGGGGAGGATGTTCCTTCTGATTTTATTCCTAAACTTGTTGGTTGCACTATTACCATCGGAAAATCTAGTGATGATGGTTCAAAAACTAACAAGGATGGTATGAAAGCTGGGGGAAATATTGATGCTGATGGGGGAATTATTGTTGACGATGATGTTCGTGTTGTTCAAGATGGTATGAAAGCTGGGGGAAATATTGATCTTGATGAGAGAAATATTGTTGATGATGCTGATCGGGGAAATTTTATTCATGTTGACGATCCTTCTGGTTCAATTCCTAAACTATTGAAAACTATATCCTCTGAAAACATTGTTGAAGATGGTTTGATAGCTAAGAAAGATGTTGATTCTGATGCTCATTCAATTTTACCTCCCAAAGATAACACTCTGACTATATGTGTTGAGGATTGTAAGCATAAATGTTCAGTTTGA

Protein sequence

>MsaT027811.1

MTFPDHHNNIFEASPQFPMNKRPIISENNHSNEFICPPPSNKETGSIFFKTRICHKFRFGNCPKGGYCNYAHGVGEIRQLPPNWKEVAGPRNDEWMQFLDDDQKIIHKMGLCKKYYNGEECPYGDKCSFLHRLRDDSWKSREAYALSIGSIGDGSNNLEGNRAVIKAARGTYWKIKLCPMWQQTGRCPFGEDCHYGHAEADYSFMMNNLKPNNTHEQPLENQNTIIEMKEAFIISPSAHGFEPTFRNAHFLKSVAASPTHQLTFNPSSSSSSPVFIPKGRPFEIKFNGFPSVLEVNVQSPFYLVQVWVWERFKNLQPRPKWINKEDHVLLKWHLVNALKIDNVRLALDSAVDNFIWRPYVKYADKCRLFYPNDEICVPFMKDLVDKQMLSFVICLRVSELVGFESIEKYLPHRVVMQFGMDQDVPGYVARFNETTAIAWENYCRSLSDTSLCYFPSRFFKSDVTTRYAKWWKKSVLGPQGFVKNDVPQNIIASSSKYRPHSEIPKLVSCGTVTFGKYSDDGLKTSNGDNIVGEDVPSDFIPKLVGCTITIGKSSDDGSKTNKDGMKAGGNIDADGGIIVDDDVRVVQDGMKAGGNIDLDERNIVDDADRGNFIHVDDPSGSIPKLLKTISSENIVEDGLIAKKDVDSDAHSILPPKDNTLTICVEDCKHKCSV*