AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027853 MtrunA17_Chr5g0427361 92.941 255 16 2 44 298 1 253 2.13e-151 424
MsaG027853 MtrunA17_Chr7g0255961 36.224 196 94 4 69 263 64 229 1.83e-31 117
MsaG027853 MtrunA17_Chr5g0412371 34.914 232 110 7 45 267 1 200 4.25e-30 113
MsaG027853 MtrunA17_Chr1g0198801 37.379 206 94 4 74 275 152 326 2.37e-27 108
MsaG027853 MtrunA17_Chr2g0325521 39.506 162 73 1 73 234 52 188 4.28e-24 98.2
MsaG027853 MtrunA17_Chr3g0113721 30.151 199 98 3 74 262 33 200 4.58e-22 92.0
MsaG027853 MtrunA17_Chr4g0041051 39.634 164 73 2 71 234 60 197 5.22e-21 90.1
MsaG027853 MtrunA17_Chr4g0045901 32.836 201 91 4 73 263 68 234 2.41e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027853 AT3G25710.1 38.835 206 82 5 76 263 128 307 2.76e-30 117
MsaG027853 AT3G56770.1 36.458 192 89 3 74 263 39 199 1.96e-29 112
MsaG027853 AT1G68810.1 40.761 184 77 3 52 234 152 304 1.45e-28 113
MsaG027853 AT2G41130.1 35.233 193 97 2 71 263 58 222 5.29e-25 101
MsaG027853 AT4G38070.1 35.465 172 78 4 72 242 83 222 4.08e-22 93.2
MsaG027853 AT2G40200.1 29.412 187 98 3 76 259 59 214 1.92e-18 83.2

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTCATTCTTTCCTTTC+TGG 0.091456 5:+53414724 None:intergenic
CAACCTCACGAGCCCGTTTC+CGG 0.268510 5:+53412155 None:intergenic
AACCCGTAGAATCTTCTTAA+AGG 0.270050 5:+53413015 None:intergenic
CGCTAACAACGACGCCTTAT+CGG 0.275455 5:+53412654 None:intergenic
GGAGAAGGAGGTAGAAGCTT+TGG 0.321763 5:-53412248 MsaT027853.1:CDS
CGTTTCCGGCCTAACAAAAT+CGG 0.353827 5:+53412169 None:intergenic
AAGAAGCATGCAAGAGTCAT+AGG 0.355128 5:-53412921 MsaT027853.1:CDS
ACAAGCAATCCGTTCGGTTC+TGG 0.360599 5:-53412371 MsaT027853.1:CDS
GAAAGCTGTTATAGAGAATA+GGG 0.375468 5:-53412215 MsaT027853.1:CDS
AAAGGTGAAGGTGGTGATAA+TGG 0.377501 5:-53412277 MsaT027853.1:CDS
TTATCACCACCTTCACCTTT+AGG 0.394483 5:+53412280 None:intergenic
TGAAAGCTGTTATAGAGAAT+AGG 0.402504 5:-53412216 MsaT027853.1:CDS
CCGTTCGGTTCTGGCGAAAC+CGG 0.414266 5:-53412362 MsaT027853.1:CDS
GGAGGTAGAAGCTTTGGAAA+GGG 0.416970 5:-53412242 MsaT027853.1:CDS
GACATATGTACAAATATAAC+AGG 0.417038 5:-53413318 MsaT027853.1:intron
GGCGTCGTTGTTAGCGGAAG+TGG 0.431203 5:-53412647 MsaT027853.1:CDS
TGAATGACCTTTGTGGTGTT+AGG 0.434067 5:+53412835 None:intergenic
CTCATTCTTTCCTTTCTGGT+CGG 0.437006 5:+53414728 None:intergenic
GATTCTACGGGTTTGAGAGC+TGG 0.437215 5:-53413005 MsaT027853.1:CDS
GCGCCGACAGCTTTCGTTGA+AGG 0.444854 5:+53414750 None:intergenic
TATTGCGGTGGTAGAGAGGA+AGG 0.446965 5:-53412460 MsaT027853.1:CDS
GACAAAGAGTGTTGTGGTGG+TGG 0.449486 5:-53412308 MsaT027853.1:CDS
GTCATCATCTCAGCTCGAAC+CGG 0.451864 5:+53412343 None:intergenic
TCGTCTGATTCTCCCGGAAA+AGG 0.459286 5:+53412496 None:intergenic
CCTCCTTTAAGAAGATTCTA+CGG 0.460188 5:-53413018 MsaT027853.1:CDS
GAGGTAGAAGCTTTGGAAAG+GGG 0.467743 5:-53412241 MsaT027853.1:CDS
GGATTGCTTGTGTTAGATCA+TGG 0.468042 5:+53412383 None:intergenic
GAAGAAGGAAGCTGATGAAA+TGG 0.473728 5:-53412605 MsaT027853.1:CDS
CTCCTTTAAGAAGATTCTAC+GGG 0.475979 5:-53413017 MsaT027853.1:CDS
TAATGGAAGAGAGGAGAAGG+AGG 0.476276 5:-53412260 MsaT027853.1:CDS
CTTGTTGTTGAGGAAGAATG+AGG 0.491130 5:-53412125 MsaT027853.1:CDS
ACACGTGAAACGGTCGAAGA+AGG 0.492375 5:-53412620 MsaT027853.1:CDS
TCTTCATGCAGCGGAGAACC+TGG 0.503192 5:-53412553 MsaT027853.1:CDS
CGATAAGGCGTCGTTGTTAG+CGG 0.504065 5:-53412653 MsaT027853.1:CDS
AGGAGGTAGAAGCTTTGGAA+AGG 0.513380 5:-53412243 MsaT027853.1:CDS
TGATAATGGAAGAGAGGAGA+AGG 0.516421 5:-53412263 MsaT027853.1:CDS
ATGGCGAATCGTCGCAATGA+CGG 0.517551 5:-53412586 MsaT027853.1:CDS
AAGTGGTGCAACACGTGAAA+CGG 0.520362 5:-53412630 MsaT027853.1:CDS
AGAAGCATGCAAGAGTCATA+GGG 0.522990 5:-53412920 MsaT027853.1:CDS
ACCTGGTTCAGTTAATTCTA+CGG 0.531153 5:-53412536 MsaT027853.1:CDS
GATGGATAATAAAGACTATG+AGG 0.533000 5:-53412983 MsaT027853.1:CDS
GCTGAGATGATGACGGTTGG+AGG 0.537478 5:-53412334 MsaT027853.1:CDS
AGGCCGGAAACGGGCTCGTG+AGG 0.537633 5:-53412158 MsaT027853.1:CDS
AGTGTGTTGTGAGGATCGAC+CGG 0.538414 5:-53412413 MsaT027853.1:CDS
GAGTTATTGCGGTGGTAGAG+AGG 0.542449 5:-53412464 MsaT027853.1:CDS
TTGACCGTTCCTTGTTGTTG+AGG 0.542986 5:-53412135 MsaT027853.1:CDS
GTTTCCGGCCTAACAAAATC+GGG 0.543564 5:+53412170 None:intergenic
AGGTGGTGATAATGGAAGAG+AGG 0.544625 5:-53412269 MsaT027853.1:CDS
GTCGCCTCGTCTGATTCTCC+CGG 0.551846 5:+53412490 None:intergenic
GTGGAGTGGCCTAAAGGTGA+AGG 0.554899 5:-53412289 MsaT027853.1:CDS
GCTGTCGGCGCCGACCAGAA+AGG 0.561414 5:-53414738 MsaT027853.1:CDS
ATTGCGGTGGTAGAGAGGAA+GGG 0.561452 5:-53412459 MsaT027853.1:CDS
TTGCGGTGGTAGAGAGGAAG+GGG 0.563178 5:-53412458 MsaT027853.1:CDS
CGAGCTGAGATGATGACGGT+TGG 0.565864 5:-53412337 MsaT027853.1:CDS
AGATGATGACGGTTGGAGGT+AGG 0.573370 5:-53412330 MsaT027853.1:CDS
AGGTTCTCCGCTGCATGAAG+AGG 0.577024 5:+53412555 None:intergenic
CTACGGGTTTGAGAGCTGGA+TGG 0.582407 5:-53413001 MsaT027853.1:CDS
ATTCCTTCAACGAAAGCTGT+CGG 0.583703 5:-53414753 MsaT027853.1:CDS
GTGGTGGTGGAGTGGCCTAA+AGG 0.584300 5:-53412295 MsaT027853.1:CDS
CTCATTCTTCCTCAACAACA+AGG 0.601988 5:+53412126 None:intergenic
CCGTAGAATCTTCTTAAAGG+AGG 0.605275 5:+53413018 None:intergenic
TCTAACACAAGCAATCCGTT+CGG 0.605327 5:-53412377 MsaT027853.1:CDS
TGACCTTTGTGGTGTTAGGA+AGG 0.606748 5:+53412839 None:intergenic
TCCGTAGAATTAACTGAACC+AGG 0.607098 5:+53412535 None:intergenic
AATGGAATGAATGACCTTTG+TGG 0.614221 5:+53412828 None:intergenic
GTTAGATCATGGTTCAGACC+CGG 0.622519 5:+53412394 None:intergenic
GTGTGTTGTGAGGATCGACC+GGG 0.625955 5:-53412412 MsaT027853.1:CDS
GAGTCTTCCTCTTCATGCAG+CGG 0.627449 5:-53412562 MsaT027853.1:CDS
CTACGGAGGTAACTGAGACA+TGG 0.628854 5:-53412519 MsaT027853.1:CDS
AGAGTCATAGGGAAGCTGAA+AGG 0.630828 5:-53412909 MsaT027853.1:CDS
CCGGTTTCGCCAGAACCGAA+CGG 0.637182 5:+53412362 None:intergenic
AGGAATCAAAACGATACAGA+TGG 0.639848 5:+53414770 None:intergenic
GTAGAGAGGAAGGGGAACCA+CGG 0.640152 5:-53412450 MsaT027853.1:CDS
GAGTGGCCTAAAGGTGAAGG+TGG 0.645085 5:-53412286 MsaT027853.1:CDS
TCTTCCTCAACAACAAGGAA+CGG 0.645939 5:+53412131 None:intergenic
AGAGTGTTGTGGTGGTGGAG+TGG 0.646685 5:-53412303 MsaT027853.1:CDS
ACTGTCACTTTCATCCTCCG+TGG 0.656210 5:+53412433 None:intergenic
TAGGACAAAGAGTGTTGTGG+TGG 0.658865 5:-53412311 MsaT027853.1:CDS
GAGGCGACAGTGAGTTATTG+CGG 0.669787 5:-53412475 MsaT027853.1:CDS
GAGAGGAAGGGGAACCACGG+AGG 0.671020 5:-53412447 MsaT027853.1:CDS
AGGTAGGACAAAGAGTGTTG+TGG 0.684138 5:-53412314 MsaT027853.1:CDS
TGGTTCAGTTAATTCTACGG+AGG 0.696147 5:-53412533 MsaT027853.1:CDS
GGTTCGAGCTGAGATGATGA+CGG 0.696578 5:-53412341 MsaT027853.1:CDS
TCTCCTTCCTAACACCACAA+AGG 0.703951 5:-53412842 MsaT027853.1:intron
GAAAGTGACAGTGTGTTGTG+AGG 0.711096 5:-53412422 MsaT027853.1:CDS
GCGACAGTGAGTTATTGCGG+TGG 0.734204 5:-53412472 MsaT027853.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 53412114 53414796 53412114 ID=MsaG027853
Chr5 mRNA 53412114 53414796 53412114 ID=MsaT027853.1;Parent=MsaG027853
Chr5 exon 53412114 53412677 53412114 ID=MsaT027853.1.exon4;Parent=MsaT027853.1
Chr5 CDS 53412114 53412677 53412114 ID=cds.MsaT027853.1;Parent=MsaT027853.1
Chr5 exon 53412843 53413066 53412843 ID=MsaT027853.1.exon3;Parent=MsaT027853.1
Chr5 CDS 53412843 53413066 53412843 ID=cds.MsaT027853.1;Parent=MsaT027853.1
Chr5 exon 53413319 53413339 53413319 ID=MsaT027853.1.exon2;Parent=MsaT027853.1
Chr5 CDS 53413319 53413339 53413319 ID=cds.MsaT027853.1;Parent=MsaT027853.1
Chr5 exon 53414709 53414796 53414709 ID=MsaT027853.1.exon1;Parent=MsaT027853.1
Chr5 CDS 53414709 53414796 53414709 ID=cds.MsaT027853.1;Parent=MsaT027853.1
Gene Sequence

>MsaT027853.1

ATGGCCATCTGTATCGTTTTGATTCCTTCAACGAAAGCTGTCGGCGCCGACCAGAAAGGAAAGAATGAGATGAGACTATTAAGTAACAACATATGTACAAATATAACAGAACCAAGAAGAAAAAAGATCATGCTTCCTCCTTTAAGAAGATTCTACGGGTTTGAGAGCTGGATGGATAATAAAGACTATGAGGAAATCAACTGTGACAATTCAAAATCTGAGAGAAAATCAAAAGAAGCATGCAAGAGTCATAGGGAAGCTGAAAGGAGACGCAGACAGAGAATCAACGCTCATCTCAACACTCTTCGCTCTCTCCTTCCTAACACCACAAAGTCCGATAAGGCGTCGTTGTTAGCGGAAGTGGTGCAACACGTGAAACGGTCGAAGAAGGAAGCTGATGAAATGGCGAATCGTCGCAATGACGGAGAGTCTTCCTCTTCATGCAGCGGAGAACCTGGTTCAGTTAATTCTACGGAGGTAACTGAGACATGGCCTTTTCCGGGAGAATCAGACGAGGCGACAGTGAGTTATTGCGGTGGTAGAGAGGAAGGGGAACCACGGAGGATGAAAGTGACAGTGTGTTGTGAGGATCGACCGGGTCTGAACCATGATCTAACACAAGCAATCCGTTCGGTTCTGGCGAAACCGGTTCGAGCTGAGATGATGACGGTTGGAGGTAGGACAAAGAGTGTTGTGGTGGTGGAGTGGCCTAAAGGTGAAGGTGGTGATAATGGAAGAGAGGAGAAGGAGGTAGAAGCTTTGGAAAGGGGTTTGAAAGCTGTTATAGAGAATAGGGCTTTTGTGGATTCTGGGTTGGGCCCGATTTTGTTAGGCCGGAAACGGGCTCGTGAGGTTGACCGTTCCTTGTTGTTGAGGAAGAATGAGGACTTTTGCTAA

Protein sequence

>MsaT027853.1

MAICIVLIPSTKAVGADQKGKNEMRLLSNNICTNITEPRRKKIMLPPLRRFYGFESWMDNKDYEEINCDNSKSERKSKEACKSHREAERRRRQRINAHLNTLRSLLPNTTKSDKASLLAEVVQHVKRSKKEADEMANRRNDGESSSSCSGEPGSVNSTEVTETWPFPGESDEATVSYCGGREEGEPRRMKVTVCCEDRPGLNHDLTQAIRSVLAKPVRAEMMTVGGRTKSVVVVEWPKGEGGDNGREEKEVEALERGLKAVIENRAFVDSGLGPILLGRKRAREVDRSLLLRKNEDFC*