AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027873


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027873 MtrunA17_Chr5g0427651 78.814 118 2 1 1 95 636 753 4.26e-57 188
MsaG027873 MtrunA17_Chr3g0132791 66.949 118 16 1 1 95 643 760 3.89e-46 157
MsaG027873 MtrunA17_Chr1g0179331 54.237 118 31 1 1 95 545 662 5.75e-35 125
MsaG027873 MtrunA17_Chr8g0392871 53.097 113 28 2 5 94 733 843 7.29e-33 119
MsaG027873 MtrunA17_Chr1g0191171 49.123 114 33 2 5 95 683 794 1.39e-30 113
MsaG027873 MtrunA17_Chr1g0151541 48.246 114 34 2 5 95 657 768 1.53e-29 110
MsaG027873 MtrunA17_Chr6g0482711 52.174 115 30 2 5 96 654 766 1.88e-29 110
MsaG027873 MtrunA17_Chr1g0171451 46.087 115 39 1 4 95 407 521 5.44e-26 100
MsaG027873 MtrunA17_Chr4g0068411 46.491 114 36 2 5 95 454 565 6.56e-25 97.1
MsaG027873 MtrunA17_Chr2g0294351 47.368 114 35 2 5 95 460 571 1.40e-24 96.3
MsaG027873 MtrunA17_Chr5g0397931 41.228 114 42 2 5 95 417 528 1.33e-21 87.8
MsaG027873 MtrunA17_Chr7g0240231 100.000 33 0 0 1 33 1 33 9.49e-19 74.7
MsaG027873 MtrunA17_Chr4g0041201 37.719 114 48 1 6 96 542 655 8.95e-18 76.6
MsaG027873 MtrunA17_Chr2g0325851 36.842 114 49 1 6 96 552 665 2.30e-17 75.5
MsaG027873 MtrunA17_Chr5g0411591 90.625 32 3 0 1 32 1 32 2.89e-15 66.2
MsaG027873 MtrunA17_Chr5g0406251 35.714 98 40 1 2 76 286 383 2.50e-14 67.0
MsaG027873 MtrunA17_Chr1g0202101 34.188 117 51 3 2 95 275 388 3.61e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027873 AT4G24480.3 68.376 117 14 1 2 95 660 776 4.86e-48 163
MsaG027873 AT4G24480.2 68.376 117 14 1 2 95 660 776 6.23e-48 163
MsaG027873 AT4G24480.1 65.574 122 14 1 2 95 660 781 2.33e-47 161
MsaG027873 AT5G03730.2 55.556 117 29 1 2 95 542 658 5.47e-35 126
MsaG027873 AT5G03730.1 55.556 117 29 1 2 95 542 658 5.47e-35 126
MsaG027873 AT1G73660.1 53.982 113 27 2 5 94 742 852 6.56e-33 120
MsaG027873 AT1G18160.1 52.212 113 29 2 5 94 709 819 3.28e-32 118
MsaG027873 AT1G08720.2 50.877 114 31 2 5 95 663 774 2.85e-31 115
MsaG027873 AT1G08720.1 50.877 114 31 2 5 95 663 774 2.94e-31 115
MsaG027873 AT5G11850.1 50.877 114 31 2 5 95 603 714 4.08e-31 115
MsaG027873 AT1G18160.2 51.304 115 31 2 5 96 709 821 9.00e-31 114
MsaG027873 AT3G06630.7 47.368 114 35 2 5 95 428 539 6.20e-24 94.7
MsaG027873 AT3G06630.6 47.368 114 35 2 5 95 344 455 6.59e-24 94.4
MsaG027873 AT3G06630.4 47.368 114 35 2 5 95 428 539 8.51e-24 94.4
MsaG027873 AT3G06630.3 47.368 114 35 2 5 95 428 539 8.51e-24 94.4
MsaG027873 AT3G06630.5 47.368 114 35 2 5 95 428 539 8.51e-24 94.4
MsaG027873 AT3G06630.2 47.368 114 35 2 5 95 428 539 9.29e-24 94.4
MsaG027873 AT3G06630.1 47.368 114 35 2 5 95 428 539 9.34e-24 94.4
MsaG027873 AT3G06620.1 46.491 114 36 2 5 95 488 599 1.14e-23 94.0
MsaG027873 AT5G49470.7 47.368 114 35 2 5 95 358 469 1.28e-23 93.6
MsaG027873 AT3G06640.2 47.368 114 35 2 5 95 440 551 1.44e-23 93.6
MsaG027873 AT3G06640.3 47.368 114 35 2 5 95 440 551 1.44e-23 93.6
MsaG027873 AT5G49470.6 47.368 114 35 2 5 95 485 596 1.63e-23 93.6
MsaG027873 AT3G06640.1 47.368 114 35 2 5 95 440 551 1.75e-23 93.6
MsaG027873 AT5G49470.5 47.368 114 35 2 5 95 485 596 1.87e-23 93.2
MsaG027873 AT5G49470.3 47.368 114 35 2 5 95 485 596 2.00e-23 93.2
MsaG027873 AT5G49470.4 47.368 114 35 2 5 95 459 570 2.47e-23 92.8
MsaG027873 AT1G67890.4 46.491 114 36 2 5 95 353 464 3.56e-23 92.4
MsaG027873 AT1G67890.1 46.491 114 36 2 5 95 481 592 3.86e-23 92.4
MsaG027873 AT5G49470.1 47.368 114 35 2 5 95 198 309 3.92e-23 92.0
MsaG027873 AT1G67890.3 46.491 114 36 2 5 95 481 592 5.86e-23 92.0
MsaG027873 AT1G67890.2 46.491 114 36 2 5 95 481 592 5.86e-23 92.0
MsaG027873 AT4G23050.1 41.228 114 42 2 5 95 461 572 7.54e-23 91.7
MsaG027873 AT4G23050.2 41.228 114 42 2 5 95 462 573 7.54e-23 91.7
MsaG027873 AT3G58640.1 34.188 117 53 2 2 95 545 660 1.07e-17 77.0
MsaG027873 AT3G58640.2 34.188 117 53 2 2 95 545 660 1.07e-17 77.0
MsaG027873 AT2G31010.1 37.391 115 49 1 5 96 513 627 1.31e-17 76.6
MsaG027873 AT2G31010.2 37.391 115 49 1 5 96 513 627 1.31e-17 76.6
MsaG027873 AT5G49470.2 45.652 92 27 1 5 73 482 573 8.25e-17 74.3
MsaG027873 AT4G35780.1 32.479 117 54 2 2 95 283 397 5.25e-14 66.2
MsaG027873 AT4G38470.3 35.714 98 40 1 2 76 281 378 2.66e-13 64.3
MsaG027873 AT2G17700.1 32.479 117 54 2 2 95 277 391 2.71e-13 64.3
MsaG027873 AT4G38470.1 35.714 98 40 1 2 76 281 378 4.34e-13 63.9
MsaG027873 AT4G38470.2 35.714 98 40 1 2 76 281 378 4.54e-13 63.5
MsaG027873 AT4G18950.2 42.647 68 36 1 29 96 199 263 1.26e-11 59.7
MsaG027873 AT4G18950.1 43.284 67 35 1 29 95 199 262 1.43e-11 59.3
MsaG027873 AT2G42640.3 31.250 96 42 2 2 74 520 614 3.24e-11 58.5
MsaG027873 AT2G42640.1 31.250 96 42 2 2 74 520 614 4.85e-11 58.2
MsaG027873 AT2G42640.4 31.250 96 42 2 2 74 482 576 5.71e-11 57.8

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTTATGCAGGATCATT+TGG 0.282685 5:-53679457 MsaT027873.1:intron
TGGATGCTGGCCGGTGAATC+AGG 0.287253 5:+53678458 None:intergenic
TCCAAATGTGGTGCTCTTCA+TGG 0.376916 5:-53678645 MsaT027873.1:CDS
CCTTGGATCGTGCATTTCAC+TGG 0.385640 5:+53678438 None:intergenic
CCCATGAAGAGCACCACATT+TGG 0.410475 5:+53678644 None:intergenic
TTATTTATGCAGGATCATTT+GGG 0.416542 5:-53679456 MsaT027873.1:intron
TGGACTGGCTTGAGATATCA+TGG 0.445535 5:-53679589 MsaT027873.1:CDS
GCCATTCGTAATCTTCCCCT+TGG 0.488605 5:+53678421 None:intergenic
ATCAAAGAACGCATTGGTGC+TGG 0.498755 5:-53679554 MsaT027873.1:intron
GTTTATTCCGCCTGATTCAC+CGG 0.500117 5:-53678468 MsaT027873.1:CDS
CCAGTGAAATGCACGATCCA+AGG 0.504528 5:-53678438 MsaT027873.1:CDS
TCGTGCATTTCACTGGATGC+TGG 0.529268 5:+53678445 None:intergenic
CCAAATGTGGTGCTCTTCAT+GGG 0.530102 5:-53678644 MsaT027873.1:CDS
AACCATCACTAGCAATGGAC+TGG 0.566572 5:-53679604 None:intergenic
GCATTTCACTGGATGCTGGC+CGG 0.576096 5:+53678449 None:intergenic
GGACAGTGTACCGTGCTGAA+TGG 0.576758 5:-53679435 MsaT027873.1:CDS
CAGTGAAATGCACGATCCAA+GGG 0.577597 5:-53678437 MsaT027873.1:CDS
CTGCGGATCAAAGAACGCAT+TGG 0.597029 5:-53679560 MsaT027873.1:CDS
TGAAGAGCACCACATTTGGA+TGG 0.617370 5:+53678648 None:intergenic
TAGTGACAGAGTATTTGCCC+AGG 0.619832 5:-53678592 MsaT027873.1:intron
TGTGGTGCTCTTCATGGGTG+CGG 0.620498 5:-53678639 MsaT027873.1:CDS
CTGATCCATGCCATTCAGCA+CGG 0.630160 5:+53679425 None:intergenic
AGATATCATGGGATGAGCTG+CGG 0.633760 5:-53679577 MsaT027873.1:CDS
TCCAAGGGGAAGATTACGAA+TGG 0.635338 5:-53678422 MsaT027873.1:CDS
GTGTACCGTGCTGAATGGCA+TGG 0.657179 5:-53679430 MsaT027873.1:CDS
ACTTGTCCGCCATCCAAATG+TGG 0.684843 5:-53678657 MsaT027873.1:CDS
ATGCTGGCCGGTGAATCAGG+CGG 0.689622 5:+53678461 None:intergenic
TCTGTCACTATAGATAGATG+AGG 0.694310 5:+53678605 None:intergenic
GGACTGGCTTGAGATATCAT+GGG 0.699073 5:-53679588 MsaT027873.1:CDS
AGAGCACCACATTTGGATGG+CGG 0.707353 5:+53678651 None:intergenic
AGTGAAATGCACGATCCAAG+GGG 0.726437 5:-53678436 MsaT027873.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 53678399 53679612 53678399 ID=MsaG027873
Chr5 mRNA 53678399 53679612 53678399 ID=MsaT027873.1;Parent=MsaG027873
Chr5 exon 53678399 53678495 53678399 ID=MsaT027873.1.exon4;Parent=MsaT027873.1
Chr5 CDS 53678399 53678495 53678399 ID=cds.MsaT027873.1;Parent=MsaT027873.1
Chr5 exon 53678593 53678693 53678593 ID=MsaT027873.1.exon3;Parent=MsaT027873.1
Chr5 CDS 53678593 53678693 53678593 ID=cds.MsaT027873.1;Parent=MsaT027873.1
Chr5 exon 53679426 53679466 53679426 ID=MsaT027873.1.exon2;Parent=MsaT027873.1
Chr5 CDS 53679426 53679466 53679426 ID=cds.MsaT027873.1;Parent=MsaT027873.1
Chr5 exon 53679555 53679612 53679555 ID=MsaT027873.1.exon1;Parent=MsaT027873.1
Chr5 CDS 53679555 53679612 53679555 ID=cds.MsaT027873.1;Parent=MsaT027873.1
Gene Sequence

>MsaT027873.1

ATGGACTGGCTTGAGATATCATGGGATGAGCTGCGGATCAAAGAACGCATTGGTGCTGGATCATTTGGGACAGTGTACCGTGCTGAATGGCATGGATCAGTTGCAATAATGAAACTTGTCCGCCATCCAAATGTGGTGCTCTTCATGGGTGCGGTTACAAAGCGTCCTCATCTATCTATAGTGACAGAGTATTTGCCCAGAGGTAGTTTATTCCGCCTGATTCACCGGCCAGCATCCAGTGAAATGCACGATCCAAGGGGAAGATTACGAATGGCTCTAGACGTTGTTTGTACATAA

Protein sequence

>MsaT027873.1

MDWLEISWDELRIKERIGAGSFGTVYRAEWHGSVAIMKLVRHPNVVLFMGAVTKRPHLSIVTEYLPRGSLFRLIHRPASSEMHDPRGRLRMALDVVCT*