AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027883


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027883 MtrunA17_Chr5g0427661 97.213 287 7 1 1 287 1 286 0.0 581
MsaG027883 MtrunA17_Chr3g0132761 60.465 258 71 8 46 275 42 296 3.75e-93 278
MsaG027883 MtrunA17_Chr3g0132751 54.595 185 76 5 74 252 69 251 2.28e-57 187
MsaG027883 MtrunA17_Chr4g0049741 51.208 207 80 8 53 245 20 219 3.26e-56 184
MsaG027883 MtrunA17_Chr4g0002231 56.701 97 28 4 152 237 42 135 1.85e-26 101
MsaG027883 MtrunA17_Chr3g0102511 39.216 102 57 3 159 259 4 101 4.34e-15 75.1
MsaG027883 MtrunA17_Chr4g0002261 68.627 51 15 1 127 176 16 66 7.81e-15 67.8
MsaG027883 MtrunA17_Chr4g0002251 67.500 40 13 0 199 238 5 44 2.63e-13 63.9
MsaG027883 MtrunA17_Chr4g0061021 52.459 61 27 1 135 195 505 563 2.53e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027883 AT4G24470.1 58.586 198 80 1 72 267 81 278 5.92e-74 229
MsaG027883 AT4G24470.2 58.586 198 80 1 72 267 81 278 5.92e-74 229
MsaG027883 AT4G24470.3 62.778 180 65 1 72 249 81 260 1.63e-73 228
MsaG027883 AT1G51600.1 59.893 187 64 4 70 245 79 265 1.07e-68 215
MsaG027883 AT1G51600.2 59.893 187 64 4 70 245 79 265 1.07e-68 215
MsaG027883 AT3G21175.1 51.452 241 84 7 38 245 21 261 3.38e-68 214
MsaG027883 AT3G21175.2 51.667 240 83 7 38 245 21 259 2.57e-67 211
MsaG027883 AT3G21175.3 47.802 182 69 6 38 194 21 201 7.73e-38 133
MsaG027883 AT2G46670.1 50.909 55 27 0 129 183 126 180 3.51e-11 61.6
MsaG027883 AT2G46790.5 50.909 55 27 0 129 183 254 308 6.49e-11 62.4
MsaG027883 AT2G46790.4 50.909 55 27 0 129 183 254 308 6.49e-11 62.4
MsaG027883 AT2G46790.1 50.909 55 27 0 129 183 411 465 6.82e-11 62.8
MsaG027883 AT2G46790.2 50.909 55 27 0 129 183 294 348 8.25e-11 62.4

Find 80 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTTGAAGTGCAAATTC+AGG 0.278085 5:-53882181 None:intergenic
ATGGCCCTCGCCGCATCATT+GGG 0.283489 5:-53885221 None:intergenic
TCCAACTCCGATGCTGTTTA+CGG 0.309806 5:+53882142 MsaT027883.1:CDS
GATGGCCCTCGCCGCATCAT+TGG 0.329482 5:-53885222 None:intergenic
GTTGTAGATGGTTGCTCAAT+AGG 0.337983 5:-53886416 None:intergenic
TCGCTTTCGCCGGCGGTGAT+TGG 0.347965 5:-53882046 None:intergenic
CTGGTCTGTACCACAACTAT+TGG 0.364949 5:-53884949 None:intergenic
GAAAGAAATACCTGCTCAAC+AGG 0.391041 5:-53886091 None:intergenic
GGTCACTTTGCAATGCTTGT+GGG 0.405446 5:+53885253 MsaT027883.1:CDS
GGCGAAAGCGACGACGGTTC+CGG 0.410307 5:+53882058 MsaT027883.1:CDS
ATGATGCGGCGAGGGCCATC+TGG 0.424046 5:+53885225 MsaT027883.1:CDS
AAGCCTCATTACTTAGGTTC+CGG 0.424048 5:+53883431 MsaT027883.1:CDS
GTCGATGCCGGCGTCGGGAC+CGG 0.425728 5:-53882077 None:intergenic
AGGTCACTTTGCAATGCTTG+TGG 0.439332 5:+53885252 MsaT027883.1:CDS
TTTCTGATGGACTGGCATCC+TGG 0.442732 5:-53884980 None:intergenic
TCAATCGGTGTTGTTACTGT+TGG 0.455881 5:+53882496 MsaT027883.1:CDS
CAATCGGTGTTGTTACTGTT+GGG 0.455920 5:+53882497 MsaT027883.1:CDS
AGATAATTCCTGATGTCTCT+TGG 0.456240 5:-53886062 None:intergenic
AGCTTACGCTGTCGTTTCGT+GGG 0.456555 5:+53882218 MsaT027883.1:CDS
ATCTTTCTTTCCTCTTCTGC+CGG 0.458420 5:-53883450 None:intergenic
ATGTAGGATGCAGCGAAAGA+AGG 0.464044 5:+53884893 MsaT027883.1:intron
CAGCTTACGCTGTCGTTTCG+TGG 0.465455 5:+53882217 MsaT027883.1:CDS
TGAGCGTCGATGCCGGCGTC+GGG 0.466145 5:-53882082 None:intergenic
TACTAGCAAATAGTTGTAGA+TGG 0.470683 5:-53886428 None:intergenic
ATCTACTGTACCTGTTGAGC+AGG 0.474916 5:+53886081 MsaT027883.1:CDS
CGGTGCCTCAACAAAGTTTG+AGG 0.475750 5:+53882552 MsaT027883.1:CDS
ATTGAGAATCGAACCTTAGC+AGG 0.477071 5:-53882268 None:intergenic
CAAGCATTGCAAAGTGACCT+TGG 0.478461 5:-53885249 None:intergenic
GCCGTAAACAGCATCGGAGT+TGG 0.479472 5:-53882143 None:intergenic
TGTAGGATGCAGCGAAAGAA+GGG 0.482115 5:+53884894 MsaT027883.1:intron
TGGCATCCTGGCTAGAATCC+TGG 0.494476 5:-53884968 None:intergenic
TCAGTACGAAACGCACGGTA+TGG 0.494591 5:+53882114 MsaT027883.1:CDS
TGACTCTGTTACGCCTGCTA+AGG 0.495608 5:+53882255 MsaT027883.1:CDS
ACCGCATTCACACCAGCATC+AGG 0.496179 5:-53882533 None:intergenic
ATCGAGAAGCCTCATTACTT+AGG 0.501589 5:+53883425 MsaT027883.1:CDS
TTTCATGTTACAGGTTCAAT+CGG 0.502821 5:+53882481 MsaT027883.1:intron
GGAACCGTCGTCGCTTTCGC+CGG 0.503198 5:-53882056 None:intergenic
TACTTAGGTTCCGGCAGAAG+AGG 0.507196 5:+53883440 MsaT027883.1:CDS
GACGGTTCCGGTCCCGACGC+CGG 0.509123 5:+53882070 MsaT027883.1:CDS
ACTACATCTAAGAAACAAGA+TGG 0.513677 5:+53884924 MsaT027883.1:CDS
TCCGATGCTGTTTACGGCAA+CGG 0.517634 5:+53882148 MsaT027883.1:CDS
TTGAGCGTCGATGCCGGCGT+CGG 0.518484 5:-53882083 None:intergenic
ATTGGTACTGTGGTGTTCAA+AGG 0.519474 5:-53882028 None:intergenic
CGACCCCAATGATGCGGCGA+GGG 0.521861 5:+53885217 MsaT027883.1:CDS
AGACCTTTCCAAGAGACATC+AGG 0.523306 5:+53886054 MsaT027883.1:CDS
TCACGAGATTTCTGATGGAC+TGG 0.530433 5:-53884988 None:intergenic
GGTTGAGAGTGTTCGTACAT+TGG 0.539771 5:-53882007 None:intergenic
ACCGTCGTCGCTTTCGCCGG+CGG 0.540258 5:-53882053 None:intergenic
TGCGATGACTAGGTTGATCA+AGG 0.549206 5:+53886344 MsaT027883.1:intron
CAAAGTGACCTTGGACCAGA+TGG 0.549647 5:-53885240 None:intergenic
TGAGAGTGTTCGTACATTGG+TGG 0.550890 5:-53882004 None:intergenic
TTGATGTTGAGCGTCGATGC+CGG 0.559465 5:-53882089 None:intergenic
TACAGACCAGGATTCTAGCC+AGG 0.560530 5:+53884962 MsaT027883.1:CDS
CAAGCTCACGAGATTTCTGA+TGG 0.565628 5:-53884993 None:intergenic
CAATAGTTGTGGTACAGACC+AGG 0.567890 5:+53884950 MsaT027883.1:CDS
GGATGTGAACAACCTGATGC+TGG 0.568670 5:+53882521 MsaT027883.1:CDS
CTGCCGGAACCTAAGTAATG+AGG 0.570375 5:-53883434 None:intergenic
ATTCCTGATGTCTCTTGGAA+AGG 0.575092 5:-53886057 None:intergenic
GGTGCCTCAACAAAGTTTGA+GGG 0.580121 5:+53882553 MsaT027883.1:CDS
ATAGGAGTGATGTTGTGTGC+AGG 0.580688 5:-53886398 None:intergenic
GCCGTTGCCGTAAACAGCAT+CGG 0.590158 5:-53882149 None:intergenic
GGCGAGGGCCATCTGGTCCA+AGG 0.590488 5:+53885232 MsaT027883.1:CDS
CAAGATGGGTCCAATAGTTG+TGG 0.595326 5:+53884939 MsaT027883.1:CDS
TTAAATAGTTGCACACATTG+TGG 0.601041 5:+53885180 MsaT027883.1:intron
CAAGGTAACAACTCAGACTG+TGG 0.601587 5:+53886362 MsaT027883.1:CDS
TCGGTGTTGTTACTGTTGGG+TGG 0.618046 5:+53882500 MsaT027883.1:CDS
TACGTCAAGAAGTTGCACTC+AGG 0.620095 5:+53883500 MsaT027883.1:CDS
ACCACCCTCAAACTTTGTTG+AGG 0.626395 5:-53882557 None:intergenic
ACCGCCGGCGAAAGCGACGA+CGG 0.634534 5:+53882052 MsaT027883.1:CDS
TAGGAGTGATGTTGTGTGCA+GGG 0.638031 5:-53886397 None:intergenic
CTACATCTAAGAAACAAGAT+GGG 0.649224 5:+53884925 MsaT027883.1:CDS
CAAAATCGACCCCAATGATG+CGG 0.651595 5:+53885211 MsaT027883.1:CDS
ACCACAGTACCAATCACCGC+CGG 0.655305 5:+53882037 MsaT027883.1:CDS
GCCTCAACAAAGTTTGAGGG+TGG 0.655336 5:+53882556 MsaT027883.1:CDS
TCGACCCCAATGATGCGGCG+AGG 0.660240 5:+53885216 MsaT027883.1:CDS
GATGCTGTTTACGGCAACGG+CGG 0.661829 5:+53882151 MsaT027883.1:CDS
ACCTGATGCTGGTGTGAATG+CGG 0.662406 5:+53882532 MsaT027883.1:CDS
GCCGGCGGTGATTGGTACTG+TGG 0.669157 5:-53882038 None:intergenic
TGGCCCTCGCCGCATCATTG+GGG 0.672900 5:-53885220 None:intergenic
CAAATTCAGTACGAAACGCA+CGG 0.681403 5:+53882109 MsaT027883.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 53881998 53886448 53881998 ID=MsaG027883
Chr5 mRNA 53881998 53886448 53881998 ID=MsaT027883.1;Parent=MsaG027883
Chr5 exon 53881998 53882276 53881998 ID=MsaT027883.1.exon1;Parent=MsaT027883.1
Chr5 CDS 53881998 53882276 53881998 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53882494 53882577 53882494 ID=MsaT027883.1.exon2;Parent=MsaT027883.1
Chr5 CDS 53882494 53882577 53882494 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53883388 53883521 53883388 ID=MsaT027883.1.exon3;Parent=MsaT027883.1
Chr5 CDS 53883388 53883521 53883388 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53884899 53885006 53884899 ID=MsaT027883.1.exon4;Parent=MsaT027883.1
Chr5 CDS 53884899 53885006 53884899 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53885188 53885293 53885188 ID=MsaT027883.1.exon5;Parent=MsaT027883.1
Chr5 CDS 53885188 53885293 53885188 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53886043 53886102 53886043 ID=MsaT027883.1.exon6;Parent=MsaT027883.1
Chr5 CDS 53886043 53886102 53886043 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Chr5 exon 53886356 53886448 53886356 ID=MsaT027883.1.exon7;Parent=MsaT027883.1
Chr5 CDS 53886356 53886448 53886356 ID=cds.MsaT027883.1;Parent=MsaT027883.1
Gene Sequence

>MsaT027883.1

ATGGAACCACCAATGTACGAACACTCTCAACCTTTGAACACCACAGTACCAATCACCGCCGGCGAAAGCGACGACGGTTCCGGTCCCGACGCCGGCATCGACGCTCAACATCAAATTCAGTACGAAACGCACGGTATGGATGATTCCAACTCCGATGCTGTTTACGGCAACGGCGGCGAAAATCCTGAATTTGCACTTCAAAGTTTTGATGAATCTGATCAGCTTACGCTGTCGTTTCGTGGGCAAGTTTATGTCTTTGACTCTGTTACGCCTGCTAAGGTTCAATCGGTGTTGTTACTGTTGGGTGGATGTGAACAACCTGATGCTGGTGTGAATGCGGTGCCTCAACAAAGTTTGAGGGTGGAATCAATGGATTTTCCTACAAAATATAGTCAACTACATCGAGAAGCCTCATTACTTAGGTTCCGGCAGAAGAGGAAAGAAAGATGCTTTGATAAGAAAGTTAGATATGAAGTACGTCAAGAAGTTGCACTCAGGATGCAGCGAAAGAAGGGTCAATTTACTACATCTAAGAAACAAGATGGGTCCAATAGTTGTGGTACAGACCAGGATTCTAGCCAGGATGCCAGTCCATCAGAAATCTCTTGCACACATTGTGGCACAAGCTCAAAATCGACCCCAATGATGCGGCGAGGGCCATCTGGTCCAAGGTCACTTTGCAATGCTTGTGGGCTTTTCTGGGCAAATAGGGGTGTCTTGAGAGACCTTTCCAAGAGACATCAGGAATTATCTACTGTACCTGTTGAGCAGGTTGATCAAGGTAACAACTCAGACTGTGGAACTACAACTATCCCTGCACACAACATCACTCCTATTGAGCAACCATCTACAACTATTTGCTAG

Protein sequence

>MsaT027883.1

MEPPMYEHSQPLNTTVPITAGESDDGSGPDAGIDAQHQIQYETHGMDDSNSDAVYGNGGENPEFALQSFDESDQLTLSFRGQVYVFDSVTPAKVQSVLLLLGGCEQPDAGVNAVPQQSLRVESMDFPTKYSQLHREASLLRFRQKRKERCFDKKVRYEVRQEVALRMQRKKGQFTTSKKQDGSNSCGTDQDSSQDASPSEISCTHCGTSSKSTPMMRRGPSGPRSLCNACGLFWANRGVLRDLSKRHQELSTVPVEQVDQGNNSDCGTTTIPAHNITPIEQPSTTIC*