AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG027952

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027952 MtrunA17_Chr5g0428331 88.649 185 17 2 1 184 1 182 4.60e-112 324
MsaG027952 MtrunA17_Chr5g0431811 54.783 115 50 2 163 275 629 743 2.77e-32 128
MsaG027952 MtrunA17_Chr3g0126391 53.763 93 41 2 173 263 100 192 4.09e-24 101
MsaG027952 MtrunA17_Chr8g0387981 59.459 74 30 0 200 273 128 201 7.90e-24 100
MsaG027952 MtrunA17_Chr7g0275531 40.299 134 71 2 197 329 111 236 5.43e-23 98.2
MsaG027952 MtrunA17_Chr8g0347251 59.091 66 27 0 204 269 136 201 3.03e-20 90.5
MsaG027952 MtrunA17_Chr7g0244981 41.667 108 57 2 161 263 75 181 3.69e-18 83.6
MsaG027952 MtrunA17_Chr7g0244971 37.963 108 62 1 161 263 75 182 1.32e-17 82.0
MsaG027952 MtrunA17_Chr8g0334541 52.239 67 32 0 199 265 98 164 3.04e-17 80.9
MsaG027952 MtrunA17_Chr3g0128671 36.975 119 60 3 160 270 82 193 3.53e-17 81.3
MsaG027952 MtrunA17_Chr2g0284321 42.169 83 46 1 179 261 10 90 8.50e-17 78.6
MsaG027952 MtrunA17_Chr8g0350621 47.692 65 34 0 199 263 106 170 2.14e-16 78.2
MsaG027952 MtrunA17_Chr5g0418781 42.857 105 53 4 169 272 90 188 3.05e-16 78.2
MsaG027952 MtrunA17_Chr2g0315361 53.968 63 29 0 204 266 148 210 9.13e-16 77.0
MsaG027952 MtrunA17_Chr7g0224801 56.897 58 22 1 204 261 93 147 1.22e-15 73.9
MsaG027952 MtrunA17_Chr1g0147881 43.182 88 44 3 177 261 233 317 1.29e-15 77.0
MsaG027952 MtrunA17_Chr8g0383351 52.542 59 25 1 204 262 160 215 1.39e-15 75.5
MsaG027952 MtrunA17_Chr4g0071521 47.059 85 41 3 178 261 219 300 2.26e-15 75.9
MsaG027952 MtrunA17_Chr2g0293481 39.394 99 54 2 167 261 226 322 2.32e-15 77.0
MsaG027952 MtrunA17_Chr3g0079691 51.515 66 26 3 196 261 274 333 3.36e-15 77.0
MsaG027952 MtrunA17_Chr7g0228941 54.237 59 25 1 203 261 51 107 3.97e-15 71.2
MsaG027952 MtrunA17_Chr4g0053281 45.882 85 42 3 177 261 241 321 4.90e-15 75.5
MsaG027952 MtrunA17_Chr7g0263531 49.275 69 29 3 194 261 179 242 7.15e-15 75.5
MsaG027952 MtrunA17_Chr7g0263531 49.123 57 26 1 205 261 362 415 8.02e-11 63.2
MsaG027952 MtrunA17_Chr2g0315381 43.902 82 45 1 186 267 127 207 1.25e-14 73.9
MsaG027952 MtrunA17_Chr1g0184691 45.122 82 40 3 193 272 281 359 1.25e-14 74.3
MsaG027952 MtrunA17_Chr3g0139021 54.237 59 25 1 203 261 313 369 1.33e-14 74.3
MsaG027952 MtrunA17_Chr8g0379461 47.692 65 32 1 197 261 69 131 1.76e-14 72.4
MsaG027952 MtrunA17_Chr5g0443131 54.098 61 25 1 204 264 157 214 1.93e-14 73.2
MsaG027952 MtrunA17_Chr3g0129361 53.448 58 24 1 204 261 144 198 2.07e-14 71.6
MsaG027952 MtrunA17_Chr7g0218081 48.387 62 28 2 200 261 224 281 2.37e-14 74.3
MsaG027952 MtrunA17_Chr7g0218081 48.276 58 27 1 204 261 410 464 7.54e-12 66.6
MsaG027952 MtrunA17_Chr7g0238921 52.542 59 25 1 204 262 124 179 2.88e-14 72.8
MsaG027952 MtrunA17_Chr4g0051881 55.172 58 23 1 204 261 178 232 3.72e-14 72.8
MsaG027952 MtrunA17_Chr5g0428351 45.588 68 37 0 205 272 184 251 4.04e-14 72.4
MsaG027952 MtrunA17_Chr5g0401821 42.353 85 45 3 178 261 173 254 4.81e-14 71.6
MsaG027952 MtrunA17_Chr2g0319961 40.566 106 51 4 173 273 47 145 5.35e-14 71.2
MsaG027952 MtrunA17_Chr8g0386241 50.847 59 26 1 204 262 103 158 5.62e-14 69.7
MsaG027952 MtrunA17_Chr5g0418191 47.059 68 32 2 194 261 141 204 6.85e-14 72.4
MsaG027952 MtrunA17_Chr5g0418191 46.552 58 28 1 204 261 320 374 4.79e-11 63.9
MsaG027952 MtrunA17_Chr8g0335811 53.448 58 24 1 204 261 154 208 8.52e-14 70.1
MsaG027952 MtrunA17_Chr2g0315371 49.206 63 32 0 204 266 141 203 1.23e-13 70.9
MsaG027952 MtrunA17_Chr7g0248721 53.125 64 24 3 204 265 141 200 2.03e-13 68.9
MsaG027952 MtrunA17_Chr7g0249311 50.000 58 25 2 204 261 179 232 5.72e-13 70.1
MsaG027952 MtrunA17_Chr7g0270191 40.404 99 49 3 204 295 145 240 5.84e-13 68.6
MsaG027952 MtrunA17_Chr2g0289181 49.153 59 28 1 203 261 13 69 7.81e-13 67.4
MsaG027952 MtrunA17_Chr5g0445311 48.276 58 27 1 204 261 364 418 7.98e-13 69.3
MsaG027952 MtrunA17_Chr3g0101131 48.276 58 27 1 204 261 415 469 1.05e-12 69.3
MsaG027952 MtrunA17_Chr3g0106491 36.697 109 53 4 194 301 188 281 1.53e-12 68.6
MsaG027952 MtrunA17_Chr3g0106491 46.552 58 28 1 204 261 365 419 5.10e-11 63.9
MsaG027952 MtrunA17_Chr1g0194481 51.724 58 25 1 204 261 158 212 1.53e-12 67.8
MsaG027952 MtrunA17_Chr5g0432161 48.276 58 26 2 204 261 244 297 2.00e-12 68.2
MsaG027952 MtrunA17_Chr5g0432161 48.276 58 27 1 204 261 421 475 6.48e-12 66.6
MsaG027952 MtrunA17_Chr2g0322391 53.448 58 25 1 205 262 350 405 3.00e-12 67.8
MsaG027952 MtrunA17_Chr4g0042831 35.106 94 50 2 198 291 139 221 3.02e-12 67.4
MsaG027952 MtrunA17_Chr4g0042831 48.276 58 27 1 204 261 278 332 1.48e-11 65.1
MsaG027952 MtrunA17_Chr4g0004911 44.828 58 29 1 204 261 184 238 5.57e-12 66.6
MsaG027952 MtrunA17_Chr7g0243891 44.928 69 34 2 193 261 205 269 5.58e-12 67.0
MsaG027952 MtrunA17_Chr3g0099641 50.877 57 26 1 205 261 252 306 5.72e-12 66.6
MsaG027952 MtrunA17_Chr2g0294231 44.737 76 33 2 204 279 203 269 5.90e-12 66.2
MsaG027952 MtrunA17_Chr4g0075201 46.575 73 27 2 205 277 268 328 5.92e-12 66.6
MsaG027952 MtrunA17_Chr1g0150281 49.123 57 27 1 205 261 141 195 6.59e-12 65.5
MsaG027952 MtrunA17_Chr2g0288871 52.632 57 25 1 205 261 306 360 7.44e-12 66.6
MsaG027952 MtrunA17_Chr7g0270831 52.632 57 25 1 205 261 217 271 8.41e-12 66.2
MsaG027952 MtrunA17_Chr8g0387501 52.632 57 25 1 205 261 130 184 1.07e-11 64.7
MsaG027952 MtrunA17_Chr3g0136961 44.615 65 34 1 197 261 131 193 1.44e-11 64.7
MsaG027952 MtrunA17_Chr8g0348311 52.632 57 25 1 205 261 156 210 1.83e-11 64.7
MsaG027952 MtrunA17_Chr4g0074001 47.368 57 27 1 205 261 360 413 2.11e-11 65.1
MsaG027952 MtrunA17_Chr5g0447701 47.368 57 27 1 205 261 253 306 4.02e-11 63.9
MsaG027952 MtrunA17_Chr7g0248691 41.379 87 42 3 175 261 132 209 4.57e-11 63.2
MsaG027952 MtrunA17_Chr1g0202461 49.123 57 27 1 205 261 262 316 5.51e-11 63.5
MsaG027952 MtrunA17_Chr6g0485431 50.000 58 25 2 205 261 155 209 6.43e-11 63.2
MsaG027952 MtrunA17_Chr7g0270051 47.458 59 29 1 203 261 52 108 6.92e-11 62.0
MsaG027952 MtrunA17_Chr4g0060271 52.632 57 25 1 205 261 256 310 7.43e-11 63.5
MsaG027952 MtrunA17_Chr4g0068541 45.763 59 29 1 204 262 208 263 9.05e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG027952 AT2G40740.1 34.473 351 149 11 1 345 11 286 1.17e-51 174
MsaG027952 AT2G40740.3 34.473 351 149 11 1 345 20 295 1.68e-51 174
MsaG027952 AT4G11070.2 52.174 92 42 2 180 270 85 175 2.72e-22 95.5
MsaG027952 AT4G11070.1 52.174 92 42 2 180 270 117 207 4.40e-22 95.5
MsaG027952 AT4G23810.1 59.420 69 28 0 200 268 154 222 6.16e-22 95.5
MsaG027952 AT5G24110.1 45.918 98 52 1 177 274 87 183 2.12e-21 93.6
MsaG027952 AT2G40740.2 42.188 128 42 5 220 345 145 242 4.18e-21 91.7
MsaG027952 AT2G46400.1 36.429 140 80 4 148 287 56 186 6.59e-20 89.4
MsaG027952 AT3G56400.1 52.174 69 33 0 204 272 120 188 3.31e-19 87.0
MsaG027952 AT1G66550.2 53.968 63 29 0 201 263 100 162 2.64e-18 83.6
MsaG027952 AT1G66550.1 53.968 63 29 0 201 263 105 167 3.60e-18 83.6
MsaG027952 AT1G66560.1 43.956 91 44 1 173 263 79 162 7.42e-18 82.4
MsaG027952 AT1G64000.1 56.897 58 22 1 204 261 114 168 6.92e-17 78.6
MsaG027952 AT2G40750.1 53.333 60 28 0 204 263 152 211 2.68e-16 79.3
MsaG027952 AT5G41570.1 56.897 58 22 1 204 261 98 152 2.91e-16 76.3
MsaG027952 AT5G01900.1 54.237 59 27 0 205 263 111 169 4.16e-16 77.8
MsaG027952 AT4G31550.2 44.048 84 44 2 178 261 220 300 4.35e-16 78.6
MsaG027952 AT4G31550.1 48.235 85 40 3 177 261 221 301 5.21e-16 78.6
MsaG027952 AT2G30590.1 32.639 144 89 5 122 261 229 368 1.03e-15 78.2
MsaG027952 AT2G24570.1 46.667 90 44 3 177 266 218 303 1.07e-15 77.4
MsaG027952 AT1G29280.1 44.086 93 41 4 175 261 42 129 1.08e-15 76.6
MsaG027952 AT2G46130.1 55.172 58 23 1 204 261 30 84 1.46e-15 72.4
MsaG027952 AT2G23320.1 41.176 85 46 3 178 261 214 295 1.91e-15 76.6
MsaG027952 AT1G69310.4 49.254 67 30 2 204 270 147 209 2.75e-15 75.9
MsaG027952 AT1G69310.2 49.254 67 30 2 204 270 147 209 2.75e-15 75.9
MsaG027952 AT1G69310.1 49.254 67 30 2 204 270 147 209 2.75e-15 75.9
MsaG027952 AT4G26440.1 48.052 77 27 3 202 278 176 239 4.06e-15 77.0
MsaG027952 AT5G45260.1 31.343 134 88 1 132 261 1132 1265 6.78e-15 76.6
MsaG027952 AT4G18170.1 27.925 265 158 9 75 337 59 292 8.33e-15 74.7
MsaG027952 AT5G28650.1 45.882 85 42 3 178 261 236 317 1.02e-14 74.7
MsaG027952 AT4G39410.1 42.857 84 38 2 204 280 223 303 1.18e-14 74.3
MsaG027952 AT1G66600.1 36.134 119 55 3 145 263 65 162 1.41e-14 73.2
MsaG027952 AT2G34830.1 43.023 86 43 2 180 261 187 270 1.53e-14 74.7
MsaG027952 AT2G34830.2 43.023 86 43 2 180 261 196 279 1.58e-14 74.7
MsaG027952 AT5G22570.1 49.153 59 30 0 205 263 111 169 1.87e-14 73.6
MsaG027952 AT3G04670.1 41.237 97 53 3 178 273 236 329 3.33e-14 73.2
MsaG027952 AT2G37260.1 43.925 107 53 3 172 278 321 420 3.55e-14 73.9
MsaG027952 AT2G37260.1 54.386 57 22 2 205 261 166 218 1.90e-13 71.6
MsaG027952 AT3G58710.1 43.478 92 43 4 175 261 39 126 3.84e-14 72.4
MsaG027952 AT2G37260.2 43.925 107 53 3 172 278 239 338 3.88e-14 73.2
MsaG027952 AT2G37260.2 54.386 57 22 2 205 261 84 136 1.98e-13 70.9
MsaG027952 AT2G37260.3 43.925 107 53 3 172 278 241 340 4.11e-14 73.2
MsaG027952 AT2G37260.3 54.386 57 22 2 205 261 86 138 2.03e-13 70.9
MsaG027952 AT2G44745.1 53.448 58 24 1 204 261 145 199 4.19e-14 71.2
MsaG027952 AT1G29860.1 55.172 58 23 1 204 261 136 190 5.46e-14 72.0
MsaG027952 AT1G80590.1 50.847 59 29 0 205 263 86 144 6.70e-14 70.9
MsaG027952 AT1G30650.1 43.023 86 43 2 180 261 189 272 6.81e-14 72.8
MsaG027952 AT2G03340.1 48.529 68 29 3 195 261 241 303 7.78e-14 72.8
MsaG027952 AT2G03340.1 40.964 83 46 1 204 286 415 494 2.42e-11 65.5
MsaG027952 AT3G01080.3 48.387 62 28 2 200 261 201 258 8.47e-14 72.0
MsaG027952 AT3G58710.2 40.426 94 48 3 173 261 35 125 1.04e-13 70.9
MsaG027952 AT3G01080.2 48.387 62 28 2 200 261 201 258 2.79e-13 71.2
MsaG027952 AT3G01080.2 46.552 58 28 1 204 261 344 398 9.95e-11 63.2
MsaG027952 AT5G46350.1 51.724 58 25 1 204 261 183 237 3.00e-13 70.5
MsaG027952 AT5G13080.1 53.448 58 24 1 204 261 67 121 3.45e-13 67.0
MsaG027952 AT3G01080.1 48.387 62 28 2 200 261 163 220 4.12e-13 70.5
MsaG027952 AT2G38470.1 43.182 88 40 4 180 261 154 237 4.27e-13 70.9
MsaG027952 AT1G13960.1 45.833 72 33 3 191 261 216 282 4.43e-13 70.5
MsaG027952 AT1G13960.1 46.552 58 28 1 204 261 409 463 8.65e-11 63.5
MsaG027952 AT1G13960.2 45.833 72 33 3 191 261 63 129 8.41e-13 69.3
MsaG027952 AT4G24240.1 41.758 91 49 3 172 261 249 336 9.17e-13 68.9
MsaG027952 AT5G52830.1 48.780 82 34 3 180 261 147 220 1.02e-12 68.9
MsaG027952 AT5G26170.1 46.552 58 28 1 204 261 113 167 1.21e-12 65.9
MsaG027952 AT4G31800.2 39.474 76 44 1 186 261 93 166 1.73e-12 67.0
MsaG027952 AT5G56270.1 45.455 77 29 3 202 278 271 334 1.94e-12 68.9
MsaG027952 AT4G04450.2 42.045 88 38 3 205 292 199 273 1.96e-12 68.6
MsaG027952 AT5G49520.1 50.000 58 26 1 204 261 221 275 1.98e-12 68.2
MsaG027952 AT4G26640.5 48.276 58 27 1 204 261 205 259 2.14e-12 68.2
MsaG027952 AT4G26640.6 48.276 58 27 1 204 261 205 259 2.14e-12 68.2
MsaG027952 AT4G26640.4 48.276 58 27 1 204 261 205 259 2.14e-12 68.2
MsaG027952 AT4G26640.3 48.276 58 27 1 204 261 205 259 2.14e-12 68.2
MsaG027952 AT4G23550.1 52.632 57 25 1 205 261 141 195 2.16e-12 67.4
MsaG027952 AT4G26640.2 48.276 58 27 1 204 261 381 435 2.16e-12 68.6
MsaG027952 AT4G26640.7 48.276 58 27 1 204 261 341 395 2.18e-12 68.6
MsaG027952 AT4G26640.1 48.276 58 27 1 204 261 309 363 2.33e-12 68.6
MsaG027952 AT4G31800.1 39.474 76 44 1 186 261 158 231 2.41e-12 67.4
MsaG027952 AT4G04450.1 42.045 88 38 3 205 292 293 367 2.82e-12 68.2
MsaG027952 AT4G30935.1 42.857 77 40 2 205 281 332 404 3.37e-12 67.8
MsaG027952 AT2G47260.1 38.462 91 53 1 179 269 149 236 7.69e-12 66.2
MsaG027952 AT4G01250.1 38.554 83 44 2 179 261 108 183 1.09e-11 65.5
MsaG027952 AT1G62300.1 52.632 57 25 1 205 261 313 367 1.76e-11 65.9
MsaG027952 AT4G01720.1 52.632 57 25 1 205 261 240 294 2.35e-11 65.5
MsaG027952 AT2G30250.1 52.632 57 24 1 205 261 329 382 3.07e-11 64.7
MsaG027952 AT5G43290.1 49.153 59 27 1 204 262 114 169 3.59e-11 63.5
MsaG027952 AT5G45050.1 42.353 85 42 2 177 261 1158 1235 3.99e-11 65.1
MsaG027952 AT5G45050.2 42.353 85 42 2 177 261 1130 1207 4.12e-11 65.1
MsaG027952 AT5G45050.4 42.353 85 42 2 177 261 1019 1096 4.35e-11 65.1
MsaG027952 AT4G22070.4 52.632 57 25 1 205 261 298 352 4.44e-11 64.7
MsaG027952 AT4G22070.3 52.632 57 25 1 205 261 298 352 4.44e-11 64.7
MsaG027952 AT4G22070.2 52.632 57 25 1 205 261 298 352 4.44e-11 64.7
MsaG027952 AT4G22070.1 52.632 57 25 1 205 261 298 352 4.44e-11 64.7
MsaG027952 AT1G55600.1 44.776 67 34 1 195 261 298 361 4.46e-11 64.3
MsaG027952 AT2G04880.2 47.368 57 27 1 205 261 284 337 4.82e-11 64.3
MsaG027952 AT2G04880.2 51.724 58 24 2 204 261 111 164 9.38e-11 63.5
MsaG027952 AT2G04880.1 47.368 57 27 1 205 261 308 361 4.99e-11 64.3
MsaG027952 AT2G04880.1 51.724 58 24 2 204 261 111 164 8.94e-11 63.5
MsaG027952 AT2G25000.2 37.500 80 48 1 182 261 81 158 5.46e-11 62.4
MsaG027952 AT2G25000.4 37.500 80 48 1 182 261 81 158 5.46e-11 62.4
MsaG027952 AT2G25000.3 37.500 80 48 1 182 261 103 180 8.66e-11 62.0

Find 94 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5:-54841202 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5:-54844029 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5:-54844061 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.259697 5:-54841254 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5:+54841580 MsaT027952.1:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5:+54843292 MsaT027952.1:CDS
TGAAATGGGTGGGAGGGATA+TGG 0.320333 5:+54841549 MsaT027952.1:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5:+54844270 MsaT027952.1:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.332908 5:+54841534 MsaT027952.1:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5:+54841361 MsaT027952.1:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5:-54844189 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5:-54844025 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5:-54841185 None:intergenic
CCTTTATCAGAAGTTACTAT+AGG 0.370143 5:+54843975 MsaT027952.1:intron
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5:+54844315 MsaT027952.1:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5:-54843283 None:intergenic
ATTAGAACACTCACATCAAA+AGG 0.383819 5:-54841448 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.384626 5:+54841509 MsaT027952.1:CDS
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5:-54841313 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5:+54841571 MsaT027952.1:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5:+54841402 MsaT027952.1:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5:+54843207 MsaT027952.1:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5:+54843281 MsaT027952.1:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5:-54841184 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5:+54844258 MsaT027952.1:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5:+54844203 MsaT027952.1:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5:-54843250 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5:-54844167 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5:-54844060 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5:+54841370 MsaT027952.1:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5:-54844188 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5:-54844142 None:intergenic
GAACATTACATCGGCCATAT+CGG 0.452060 5:-54844284 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5:-54844133 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.454574 5:+54841535 MsaT027952.1:CDS
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5:-54844179 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5:-54841116 None:intergenic
GGTGGTGGTGGATATGATGA+TGG 0.483740 5:-54844121 None:intergenic
TAAGGTCTAGGGAAACTTTG+AGG 0.489231 5:+54841521 MsaT027952.1:CDS
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5:-54844202 None:intergenic
CGTTCAGTTGTTAAGGTCTA+GGG 0.489911 5:+54841510 MsaT027952.1:CDS
GGACCCTCTACTTCAAGATT+CGG 0.492644 5:+54844244 MsaT027952.1:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5:-54844213 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5:+54844220 MsaT027952.1:CDS
TCTAGGGAAACTTTGAGGAT+TGG 0.504126 5:+54841526 MsaT027952.1:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5:+54843244 MsaT027952.1:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5:+54841503 MsaT027952.1:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5:+54844214 MsaT027952.1:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5:+54844186 MsaT027952.1:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5:-54841201 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5:+54844067 MsaT027952.1:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5:-54843256 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5:+54841364 MsaT027952.1:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5:+54843271 MsaT027952.1:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5:-54841194 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5:+54841481 MsaT027952.1:CDS
AAGGTTTAGATAGATCAAAA+GGG 0.540645 5:+54841572 MsaT027952.1:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5:+54841469 MsaT027952.1:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5:+54844172 MsaT027952.1:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5:-54844147 None:intergenic
TGCGGTGAACCACGACCTCA+GGG 0.552440 5:+54844204 MsaT027952.1:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5:-54844199 None:intergenic
TACTTGGCCGCAAGCACCCA+AGG 0.555851 5:+54843326 MsaT027952.1:CDS
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5:-54844139 None:intergenic
TAATTTACCTTGGGTGCTTG+CGG 0.559902 5:-54843333 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5:+54841538 MsaT027952.1:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5:+54844223 MsaT027952.1:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5:+54841539 MsaT027952.1:CDS
GGCCGATGTAATGTTCAACT+CGG 0.582893 5:+54844291 MsaT027952.1:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5:+54844173 MsaT027952.1:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5:+54841542 MsaT027952.1:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5:-54844247 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5:-54841447 None:intergenic
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5:+54844176 MsaT027952.1:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5:+54844304 MsaT027952.1:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.607995 5:+54841543 MsaT027952.1:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5:-54841323 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5:+54844360 MsaT027952.1:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5:-54844136 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5:+54844292 MsaT027952.1:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5:-54844248 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5:-54843280 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5:+54841472 MsaT027952.1:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5:+54841475 MsaT027952.1:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5:-54844277 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5:+54844208 MsaT027952.1:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5:+54841484 MsaT027952.1:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5:+54841171 MsaT027952.1:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5:+54844261 MsaT027952.1:CDS
ATGGGTGGGAGGGATATGGA+AGG 0.678067 5:+54841553 MsaT027952.1:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5:-54844219 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5:-54844293 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5:+54844205 MsaT027952.1:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5:-54841198 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 54841118 54844393 54841118 ID=MsaG027952
Chr5 mRNA 54841118 54844393 54841118 ID=MsaT027952.1;Parent=MsaG027952
Chr5 exon 54841118 54841650 54841118 ID=MsaT027952.1.exon1;Parent=MsaT027952.1
Chr5 CDS 54841118 54841650 54841118 ID=cds.MsaT027952.1;Parent=MsaT027952.1
Chr5 exon 54843210 54843347 54843210 ID=MsaT027952.1.exon2;Parent=MsaT027952.1
Chr5 CDS 54843210 54843347 54843210 ID=cds.MsaT027952.1;Parent=MsaT027952.1
Chr5 exon 54843985 54844393 54843985 ID=MsaT027952.1.exon3;Parent=MsaT027952.1
Chr5 CDS 54843985 54844393 54843985 ID=cds.MsaT027952.1;Parent=MsaT027952.1
Gene Sequence

>MsaT027952.1

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGTTGTCATCACAACATGACACAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGAAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATATGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGCACCCAAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCTTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>MsaT027952.1

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMLSSQHDTTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRETLRIGEMGGRDMEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKHPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN*