AlfalfaGEDB | MsaG028214

M. sativa ssp. caerulea / MsaG028214

KEGG: K13945 K21994
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028214	XP_003616452.1	96.875	192	5	1	1	191	1	192	5.71e-135	385

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028214	sp\|Q9LHS8\|LBD33_ARATH	51.064	188	73	4	1	186	1	171	3.05e-57	181

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028214	tr\|G7KGT3\|G7KGT3_MEDTR	96.875	192	5	1	1	191	1	192	2.73e-135	385

TFs/TRs:

Gene ID	Type	Classification
MsaG028214	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG006203	MsaG028214	0.815563	1.501703e-51	2.944567e-49
MsaG007848	MsaG028214	0.804498	3.542109e-49	5.307854e-47
MsaG044515	MsaG028214	0.831924	2.288555e-55	7.001841e-53
MsaG045833	MsaG028214	0.808043	6.396907e-50	1.042094e-47
MsaG003075	MsaG028214	0.818702	2.980881e-52	6.335862e-50
MsaG013523	MsaG028214	0.818134	4.004383e-52	8.386876e-50
MsaG016842	MsaG028214	0.814375	2.747271e-51	5.227902e-49
MsaG019414	MsaG028214	0.807494	8.359049e-50	1.343869e-47
MsaG028014	MsaG028214	0.800078	2.850725e-48	3.861122e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028214	MtrunA17_Chr5g0435641	96.875	192	5	1	1	191	1	192	5.25e-139	385
MsaG028214	MtrunA17_Chr7g0261031	62.500	120	45	0	1	120	2	121	1.88e-52	167
MsaG028214	MtrunA17_Chr1g0184271	69.608	102	31	0	8	109	13	114	1.52e-49	160
MsaG028214	MtrunA17_Chr7g0260971	64.000	100	36	0	2	101	9	108	1.66e-45	149
MsaG028214	MtrunA17_Chr6g0460951	53.782	119	55	0	6	124	39	157	1.46e-44	147
MsaG028214	MtrunA17_Chr8g0351721	59.434	106	43	0	5	110	16	121	6.19e-44	145
MsaG028214	MtrunA17_Chr8g0351701	62.264	106	40	0	8	113	24	129	6.55e-44	145
MsaG028214	MtrunA17_Chr1g0184281	48.611	144	61	2	5	148	13	143	1.64e-42	141
MsaG028214	MtrunA17_Chr7g0245871	64.646	99	35	0	8	106	28	126	1.03e-41	140
MsaG028214	MtrunA17_Chr7g0245861	63.043	92	32	2	5	94	10	101	6.79e-38	127
MsaG028214	MtrunA17_Chr7g0227511	55.670	97	42	1	6	102	10	105	3.70e-34	119
MsaG028214	MtrunA17_Chr3g0114061	54.082	98	44	1	6	103	7	103	1.53e-33	117
MsaG028214	MtrunA17_Chr5g0437571	38.824	170	77	4	3	167	2	149	2.30e-33	117
MsaG028214	MtrunA17_Chr2g0311601	54.545	99	44	1	3	101	9	106	4.18e-33	116
MsaG028214	MtrunA17_Chr4g0058651	47.368	114	59	1	4	117	8	120	6.43e-33	116
MsaG028214	MtrunA17_Chr3g0106101	47.934	121	56	3	3	117	2	121	3.12e-32	114
MsaG028214	MtrunA17_Chr6g0449641	52.577	97	45	1	6	102	8	103	3.38e-32	113
MsaG028214	MtrunA17_Chr3g0117121	51.485	101	48	1	2	102	5	104	4.27e-32	114
MsaG028214	MtrunA17_Chr4g0043421	50.495	101	49	1	3	103	11	110	6.81e-32	113
MsaG028214	MtrunA17_Chr5g0437401	44.340	106	58	1	5	110	9	113	1.91e-30	109
MsaG028214	MtrunA17_Chr5g0402611	42.754	138	74	2	6	143	7	139	1.97e-30	113
MsaG028214	MtrunA17_Chr3g0097221	37.278	169	88	5	1	160	1	160	2.20e-30	112
MsaG028214	MtrunA17_Chr3g0129071	51.515	99	47	1	6	104	46	143	2.33e-30	111
MsaG028214	MtrunA17_Chr5g0438091	50.505	99	48	1	6	104	38	135	2.42e-30	110
MsaG028214	MtrunA17_Chr6g0449651	51.546	97	46	1	6	102	66	161	3.61e-30	109
MsaG028214	MtrunA17_Chr3g0113891	47.959	98	50	1	6	103	13	109	4.45e-30	109
MsaG028214	MtrunA17_Chr6g0460941	53.191	94	44	0	1	94	1	94	6.32e-30	105
MsaG028214	MtrunA17_Chr3g0115431	46.667	105	55	1	5	109	7	110	6.41e-30	109
MsaG028214	MtrunA17_Chr4g0030001	46.018	113	52	3	6	117	23	127	1.81e-27	102
MsaG028214	MtrunA17_Chr5g0432451	53.659	82	37	1	6	87	42	122	1.44e-25	98.6
MsaG028214	MtrunA17_Chr1g0200201	46.809	94	49	1	8	101	73	165	2.38e-25	98.2
MsaG028214	MtrunA17_Chr8g0353981	50.575	87	42	1	1	87	1	86	6.16e-25	97.1
MsaG028214	MtrunA17_Chr7g0225111	42.308	104	59	1	6	109	4	106	4.83e-24	91.7
MsaG028214	MtrunA17_Chr5g0444351	42.157	102	58	1	8	109	6	106	1.06e-23	91.7
MsaG028214	MtrunA17_Chr4g0059901	38.384	99	60	1	3	101	6	103	9.28e-20	84.0
MsaG028214	MtrunA17_Chr4g0040831	32.331	133	88	2	3	135	11	141	1.59e-19	82.0
MsaG028214	MtrunA17_Chr8g0342351	35.922	103	65	1	7	109	12	113	1.86e-19	83.2
MsaG028214	MtrunA17_Chr6g0452691	35.922	103	64	2	8	109	10	111	1.41e-17	76.6
MsaG028214	MtrunA17_Chr8g0374601	37.255	102	63	1	8	109	27	127	2.86e-17	77.8
MsaG028214	MtrunA17_Chr8g0374591	37.864	103	63	1	8	110	9	110	2.91e-17	74.3
MsaG028214	MtrunA17_Chr6g0452671	39.506	81	48	1	7	87	11	90	3.30e-16	73.6
MsaG028214	MtrunA17_Chr6g0452661	32.381	105	69	2	6	109	8	111	6.64e-16	73.6
MsaG028214	MtrunA17_Chr2g0330201	32.323	99	66	1	3	101	15	112	6.74e-16	73.9
MsaG028214	MtrunA17_Chr5g0439111	33.654	104	67	2	7	109	9	111	2.40e-14	68.9
MsaG028214	MtrunA17_Chr8g0374581	35.789	95	59	2	8	101	9	102	1.18e-13	67.0
MsaG028214	MtrunA17_Chr4g0055441	30.097	103	70	2	8	109	3	104	4.40e-13	63.9
MsaG028214	MtrunA17_Chr4g0046201	31.111	90	61	1	6	95	5	93	1.74e-12	64.3
MsaG028214	MtrunA17_Chr2g0326041	52.083	48	22	1	6	53	32	78	1.91e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028214	AT5G06080.1	51.064	188	73	4	1	186	1	171	3.10e-58	181
MsaG028214	AT3G58190.1	68.868	106	33	0	8	113	12	117	1.15e-50	163
MsaG028214	AT2G42440.1	60.656	122	43	1	1	117	1	122	1.34e-49	161
MsaG028214	AT2G42430.1	45.304	181	76	3	3	166	11	185	5.92e-47	154
MsaG028214	AT4G00210.1	64.000	100	36	0	2	101	6	105	3.20e-46	152
MsaG028214	AT3G03760.1	60.714	112	44	0	6	117	50	161	3.34e-46	153
MsaG028214	AT2G31310.1	62.500	104	39	0	1	104	1	104	7.00e-45	147
MsaG028214	AT2G45420.1	64.356	101	36	0	10	110	40	140	4.40e-43	145
MsaG028214	AT4G00220.1	60.396	101	40	0	10	110	20	120	6.66e-41	138
MsaG028214	AT2G45410.1	59.783	92	37	0	10	101	19	110	3.42e-38	130
MsaG028214	AT3G27650.1	55.670	97	42	1	6	102	38	133	3.11e-34	119
MsaG028214	AT5G63090.4	48.276	116	59	1	4	119	8	122	3.19e-33	117
MsaG028214	AT5G63090.3	48.276	116	59	1	4	119	8	122	3.19e-33	117
MsaG028214	AT5G63090.2	48.276	116	59	1	4	119	8	122	3.19e-33	117
MsaG028214	AT5G63090.1	48.276	116	59	1	4	119	8	122	3.19e-33	117
MsaG028214	AT2G30130.1	51.923	104	48	2	1	103	1	103	3.38e-32	115
MsaG028214	AT1G31320.1	53.261	92	42	1	3	94	9	99	6.13e-31	111
MsaG028214	AT5G66870.1	50.000	102	50	1	1	102	1	101	1.06e-29	111
MsaG028214	AT2G28500.1	45.133	113	56	2	6	113	54	165	2.25e-29	108
MsaG028214	AT2G30340.2	51.515	99	47	1	6	104	52	149	3.53e-29	109
MsaG028214	AT2G30340.1	51.515	99	47	1	6	104	51	148	4.31e-29	109
MsaG028214	AT3G11090.1	44.037	109	59	2	6	113	10	117	7.19e-29	105
MsaG028214	AT1G07900.1	45.536	112	53	2	6	117	32	135	8.61e-29	106
MsaG028214	AT3G26660.1	45.833	96	51	1	6	101	4	98	1.12e-28	103
MsaG028214	AT1G16530.1	38.462	143	84	3	6	147	13	152	1.39e-28	105
MsaG028214	AT3G26620.1	45.833	96	51	1	6	101	4	98	1.65e-28	103
MsaG028214	AT2G23660.2	47.917	96	49	1	6	101	4	98	9.78e-27	103
MsaG028214	AT2G23660.3	47.917	96	49	1	6	101	4	98	9.78e-27	103
MsaG028214	AT2G23660.1	47.917	96	49	1	6	101	4	98	9.78e-27	103
MsaG028214	AT1G65620.1	56.098	82	35	1	6	87	8	88	5.50e-26	99.4
MsaG028214	AT1G65620.4	56.098	82	35	1	6	87	8	88	5.50e-26	99.4
MsaG028214	AT1G65620.3	56.098	82	35	1	6	87	8	88	5.50e-26	99.4
MsaG028214	AT1G65620.5	56.098	82	35	1	6	87	8	88	5.50e-26	99.4
MsaG028214	AT1G65620.2	56.098	82	35	1	6	87	8	88	5.50e-26	99.4
MsaG028214	AT2G40470.2	51.220	82	39	1	6	87	19	99	5.44e-25	96.7
MsaG028214	AT2G40470.1	51.220	82	39	1	6	87	44	124	7.81e-25	97.1
MsaG028214	AT3G50510.1	40.708	113	66	1	1	113	6	117	3.89e-24	94.4
MsaG028214	AT3G50510.2	40.708	113	66	1	1	113	6	117	3.89e-24	94.4
MsaG028214	AT3G13850.1	36.893	103	64	1	7	109	36	137	7.18e-20	84.7
MsaG028214	AT5G35900.1	39.796	98	56	3	6	102	4	99	5.92e-18	78.6
MsaG028214	AT3G47870.1	32.075	106	71	1	3	108	32	136	1.32e-17	79.3
MsaG028214	AT1G06280.1	36.842	76	47	1	7	82	24	98	4.19e-16	73.6
MsaG028214	AT1G72980.1	31.683	101	68	1	3	103	9	108	9.72e-14	67.4
MsaG028214	AT4G22700.1	33.333	96	61	2	8	102	6	99	4.56e-12	62.4

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGCGGTGCATAAGGTTTA+TGG	0.161843	5:-59062684	MsaT028214.1:CDS
CGCATGCAAGATCCTATTTA+TGG	0.202738	5:-59062573	MsaT028214.1:CDS
ATGATCCAAAACTTTGATTA+TGG	0.255926	5:+59061741	None:intergenic
TATTACCATAATCAAAGTTT+TGG	0.272555	5:-59061746	MsaT028214.1:CDS
CAAGAGGAGATAGATATTCT+TGG	0.321470	5:-59061851	MsaT028214.1:CDS
CTTTGGAAAATGACAGGGTT+TGG	0.324866	5:-59062804	None:intergenic
GGATTTCCAGAGCCTTTATA+TGG	0.325475	5:-59061686	MsaT028214.1:CDS
TCTGGAAATCCTTGTGCATT+AGG	0.337625	5:+59061698	None:intergenic
ACATTAGAGAAGTTCTTATC+AGG	0.342216	5:-59061647	MsaT028214.1:CDS
TGAGCAACACAACCATAAAT+AGG	0.353770	5:+59062561	None:intergenic
GCACCAATGAACTCAAATAA+TGG	0.356387	5:-59061776	MsaT028214.1:CDS
CACCCTTGGTTCAAGCATAA+TGG	0.370662	5:-59061599	MsaT028214.1:CDS
GTTCCATTATTTGAGTTCAT+TGG	0.371818	5:+59061773	None:intergenic
GCCTGTTCATAGGAGAAGTA+TGG	0.380890	5:+59062720	None:intergenic
GAGTTGGAATCACCATATAA+AGG	0.394155	5:+59061674	None:intergenic
TCCATACTTCTCCTATGAAC+AGG	0.425422	5:-59062721	MsaT028214.1:CDS
CTTCTCTAATGTATTTGAGT+TGG	0.427053	5:+59061658	None:intergenic
CACTATATCTTATGAAGCCT+TGG	0.435812	5:-59062598	MsaT028214.1:CDS
ATGAGTTGAAGCCTGTTCAT+AGG	0.437710	5:+59062710	None:intergenic
GAATCACCATATAAAGGCTC+TGG	0.448083	5:+59061680	None:intergenic
ATAAAACTTTGGAAAATGAC+AGG	0.453003	5:-59062810	None:intergenic
CACTAGCACACTTTCTCCTT+AGG	0.465254	5:+59062757	None:intergenic
AGGGCTTCTTGAACCACCCT+TGG	0.469455	5:-59061613	MsaT028214.1:CDS
TATCTATGCATTGCAACATC+AGG	0.488764	5:-59062538	MsaT028214.1:intron
GTGGAGCATGCAAGTTCCTA+AGG	0.513337	5:-59062773	MsaT028214.1:CDS
CTCCATTATGCTTGAACCAA+GGG	0.523653	5:+59061597	None:intergenic
AGGATCTTGCATGCGAGCCA+AGG	0.545581	5:+59062581	None:intergenic
AGATATTCTTGGAAGTGTAA+TGG	0.550072	5:-59061840	MsaT028214.1:CDS
TCTCCATTATGCTTGAACCA+AGG	0.563541	5:+59061596	None:intergenic
TAATGGAGACATGAAGAACT+AGG	0.571172	5:-59061582	None:intergenic
ACAGGGTTTGGCTCATCATG+TGG	0.589293	5:-59062792	MsaT028214.1:CDS
CATTATGCTTGAACCAAGGG+TGG	0.607660	5:+59061600	None:intergenic
AGTGTTAGTGTTGTGAATTG+TGG	0.611640	5:-59061809	MsaT028214.1:CDS
GCAGGTTGCTAGCTTGCAAG+AGG	0.615316	5:-59061867	MsaT028214.1:intron
CTTATCAGGAATTGATCAAG+AGG	0.617949	5:-59061633	MsaT028214.1:CDS
TAAAACTTTGGAAAATGACA+GGG	0.628064	5:-59062809	None:intergenic
TTATCAGGAATTGATCAAGA+GGG	0.643489	5:-59061632	MsaT028214.1:CDS
GGCTTCATAAGATATAGTGA+TGG	0.646623	5:+59062602	None:intergenic
GAGCTAATTCCTAATGCACA+AGG	0.651993	5:-59061707	MsaT028214.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	59061583	59062817	59061583	ID=MsaG028214
Chr5	mRNA	59061583	59062817	59061583	ID=MsaT028214.1;Parent=MsaG028214
Chr5	exon	59061583	59061885	59061583	ID=MsaT028214.1.exon2;Parent=MsaT028214.1
Chr5	CDS	59061583	59061885	59061583	ID=cds.MsaT028214.1;Parent=MsaT028214.1
Chr5	exon	59062539	59062817	59062539	ID=MsaT028214.1.exon1;Parent=MsaT028214.1
Chr5	CDS	59062539	59062817	59062539	ID=cds.MsaT028214.1;Parent=MsaT028214.1

Gene Sequence

>MsaT028214.1

ATGACAGGGTTTGGCTCATCATGTGGAGCATGCAAGTTCCTAAGGAGAAAGTGTGCTAGTGATTGTGTTTTTGCTCCATACTTCTCCTATGAACAGGCTTCAACTCATTTTTCAGCGGTGCATAAGGTTTATGGAGCTAGTAATGTCTCTAGACTATTGTCACATCTTCCTATTCAAAATAGAAGTGATGCTGCCATCACTATATCTTATGAAGCCTTGGCTCGCATGCAAGATCCTATTTATGGTTGTGTTGCTCATATCTATGCATTGCAACATCAGGTTGCTAGCTTGCAAGAGGAGATAGATATTCTTGGAAGTGTAATGGCAAATTCAAGTGTTAGTGTTGTGAATTGTGGCAATGTTCAAGCACCAATGAACTCAAATAATGGAACACAATATTACCATAATCAAAGTTTTGGATCATACTTGGATATGGAGCTAATTCCTAATGCACAAGGATTTCCAGAGCCTTTATATGGTGATTCCAACTCAAATACATTAGAGAAGTTCTTATCAGGAATTGATCAAGAGGGCTTCTTGAACCACCCTTGGTTCAAGCATAATGGAGACATGAAGAACTAG

Protein sequence

>MsaT028214.1

MTGFGSSCGACKFLRRKCASDCVFAPYFSYEQASTHFSAVHKVYGASNVSRLLSHLPIQNRSDAAITISYEALARMQDPIYGCVAHIYALQHQVASLQEEIDILGSVMANSSVSVVNCGNVQAPMNSNNGTQYYHNQSFGSYLDMELIPNAQGFPEPLYGDSNSNTLEKFLSGIDQEGFLNHPWFKHNGDMKN*