AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028229


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028229 MtrunA17_Chr5g0435411 91.667 72 6 0 1 72 1 72 1.82e-44 142
MsaG028229 MtrunA17_Chr5g0435401 75.556 90 22 0 93 182 177 266 1.39e-42 147
MsaG028229 MtrunA17_Chr5g0435401 63.636 88 29 2 104 190 264 349 1.19e-24 99.4
MsaG028229 MtrunA17_Chr5g0435431 87.500 72 9 0 1 72 1 72 9.05e-42 135
MsaG028229 MtrunA17_Chr1g0192841 58.261 115 37 2 87 190 38 152 1.42e-31 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028229 AT5G02220.1 50.000 60 28 1 8 67 12 69 4.05e-13 62.4

Find 40 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGATTCCCTTTCCTTTAT+AGG 0.104103 5:+59306596 None:intergenic
TTTGAAAATGGTTATGAAAT+TGG 0.137150 5:-59306792 None:intergenic
CAGACTCTTCAGTGCTCTTT+TGG 0.248679 5:+59304694 None:intergenic
TTATATCCACTCTGACCTAT+TGG 0.347768 5:+59304995 None:intergenic
ATCCGACAACCGCTGTGTTT+TGG 0.358584 5:+59306746 None:intergenic
AAAGGAAAGCAAGGAGATAA+AGG 0.375471 5:-59304739 MsaT028229.1:CDS
TTCTTACTTAAAGCAGGAGT+TGG 0.403219 5:-59305088 MsaT028229.1:CDS
GTGCTTATGCTGCAATACTT+TGG 0.423903 5:-59305112 MsaT028229.1:CDS
TGGAGATTGAAGAAGGTTGC+CGG 0.426728 5:-59306772 MsaT028229.1:CDS
GTTGTCAGACAAGGAGAAAC+AGG 0.430794 5:-59304876 MsaT028229.1:intron
ATGAAATTGGAGATTGAAGA+AGG 0.432769 5:-59306779 MsaT028229.1:CDS
TTTGGATTCTTACTTAAAGC+AGG 0.455789 5:-59305094 MsaT028229.1:CDS
AAACGGAGACGTGCTCCAAT+AGG 0.460049 5:-59305010 MsaT028229.1:CDS
TTTCATGTAGCCATATGAAA+AGG 0.472708 5:-59304757 MsaT028229.1:intron
TCGTCTAAAAGCTGATCATC+AGG 0.483966 5:-59304948 MsaT028229.1:CDS
GGTTTATTCAACGAAGAAGA+AGG 0.495785 5:-59306678 MsaT028229.1:CDS
TTCTTCGTTGAATAAACCAC+CGG 0.508962 5:+59306683 None:intergenic
CACCAAAACACAGCGGTTGT+CGG 0.521171 5:-59306748 MsaT028229.1:CDS
ATAACAAAGAAAAGAAGAAA+CGG 0.522686 5:-59305027 MsaT028229.1:CDS
GACGAGCACATTCCTGCTTG+AGG 0.523607 5:+59304966 None:intergenic
TAGACATGGCTATTGATGCT+TGG 0.537672 5:-59304904 MsaT028229.1:CDS
GAAGAAGAAGGCCCCACCGA+AGG 0.545060 5:-59306666 MsaT028229.1:CDS
GGATTGAAGTAACCATCCTT+CGG 0.555826 5:+59306650 None:intergenic
GCCATATGAAAAGGAAAGCA+AGG 0.558682 5:-59304748 MsaT028229.1:CDS
GCCCGCGACTATGTGTCCCT+CGG 0.570147 5:-59306720 MsaT028229.1:CDS
TGAAGTAACCATCCTTCGGT+GGG 0.570885 5:+59306654 None:intergenic
GCCGAGGGACACATAGTCGC+GGG 0.578000 5:+59306719 None:intergenic
AAGAAGGCCCCACCGAAGGA+TGG 0.585344 5:-59306662 MsaT028229.1:CDS
ATATAATATATTCCTCAAGC+AGG 0.589592 5:-59304978 MsaT028229.1:CDS
GCTGATCATCAGGACATATG+TGG 0.590919 5:-59304938 MsaT028229.1:CDS
TTGAAGTAACCATCCTTCGG+TGG 0.600524 5:+59306653 None:intergenic
TGGCAGAGAAATTGTAGACA+TGG 0.605105 5:-59304918 MsaT028229.1:CDS
TGCCGAGGGACACATAGTCG+CGG 0.615823 5:+59306718 None:intergenic
AAAATAAGCTCCAAATCAGG+GGG 0.654554 5:+59306629 None:intergenic
TTGGAACAAGTTGTCAGACA+AGG 0.660817 5:-59304885 MsaT028229.1:CDS
AGTAGCTCGTGTGAATTCAG+AGG 0.669108 5:-59305134 MsaT028229.1:CDS
GAAGTAACCATCCTTCGGTG+GGG 0.675290 5:+59306655 None:intergenic
CGTGCTCCAATAGGTCAGAG+TGG 0.690376 5:-59305001 MsaT028229.1:CDS
GAGGGACACATAGTCGCGGG+CGG 0.723461 5:+59306722 None:intergenic
TGCCGGACACCAAAACACAG+CGG 0.804931 5:-59306755 MsaT028229.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 59304689 59306807 59304689 ID=MsaG028229
Chr5 mRNA 59304689 59306807 59304689 ID=MsaT028229.1;Parent=MsaG028229
Chr5 exon 59304689 59304769 59304689 ID=MsaT028229.1.exon3;Parent=MsaT028229.1
Chr5 CDS 59304689 59304769 59304689 ID=cds.MsaT028229.1;Parent=MsaT028229.1
Chr5 exon 59304877 59305162 59304877 ID=MsaT028229.1.exon2;Parent=MsaT028229.1
Chr5 CDS 59304877 59305162 59304877 ID=cds.MsaT028229.1;Parent=MsaT028229.1
Chr5 exon 59306596 59306807 59306596 ID=MsaT028229.1.exon1;Parent=MsaT028229.1
Chr5 CDS 59306596 59306807 59306596 ID=cds.MsaT028229.1;Parent=MsaT028229.1
Gene Sequence

>MsaT028229.1

ATGGTTATGAAATTGGAGATTGAAGAAGGTTGCCGGACACCAAAACACAGCGGTTGTCGGATACCGCCCGCGACTATGTGTCCCTCGGCACCGAAAAAGAAGCCGGTGGTTTATTCAACGAAGAAGAAGGCCCCACCGAAGGATGGTTACTTCAATCCCCCTGATTTGGAGCTTATTTTTAGAGTTTTGCCTATAAAGGAAAGGGAATCTAAATTAAAAGTAGCTCGTGTGAATTCAGAGGTGCTTATGCTGCAATACTTTGGATTCTTACTTAAAGCAGGAGTTGGATATCAAGAACATCAAGTGTTTCAACAAGCACCCACAAATAACAAAGAAAAGAAGAAACGGAGACGTGCTCCAATAGGTCAGAGTGGATATAATATATTCCTCAAGCAGGAATGTGCTCGTCTAAAAGCTGATCATCAGGACATATGTGGCAGAGAAATTGTAGACATGGCTATTGATGCTTGGAACAAGTTGTCAGACAAGGAGAAACAGCCATATGAAAAGGAAAGCAAGGAGATAAAGGAAAAAATTAAAGAAACAATGATCCAAAAGAGCACTGAAGAGTCTGTGTAA

Protein sequence

>MsaT028229.1

MVMKLEIEEGCRTPKHSGCRIPPATMCPSAPKKKPVVYSTKKKAPPKDGYFNPPDLELIFRVLPIKERESKLKVARVNSEVLMLQYFGFLLKAGVGYQEHQVFQQAPTNNKEKKKRRRAPIGQSGYNIFLKQECARLKADHQDICGREIVDMAIDAWNKLSDKEKQPYEKESKEIKEKIKETMIQKSTEESV*