Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028229 | AES99377.1 | 91.667 | 72 | 6 | 0 | 1 | 72 | 1 | 72 | 1.98e-40 | 142 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028229 | sp|Q1JPP8|SMR4_ARATH | 50.000 | 60 | 28 | 1 | 8 | 67 | 12 | 69 | 3.99e-12 | 62.4 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028229 | tr|G7KBS4|G7KBS4_MEDTR | 91.667 | 72 | 6 | 0 | 1 | 72 | 1 | 72 | 9.47e-41 | 142 |
Gene ID | Type | Classification |
---|---|---|
MsaG028229 | TF | C2C2-YABBY |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000346 | MsaG028229 | 0.809254 | 3.534898e-50 | 5.928741e-48 |
MsaG000431 | MsaG028229 | 0.853309 | 5.001396e-61 | 3.042114e-58 |
MsaG001237 | MsaG028229 | 0.821661 | 6.306545e-53 | 1.449399e-50 |
MsaG001248 | MsaG028229 | 0.809287 | 3.478170e-50 | 5.838166e-48 |
MsaG001531 | MsaG028229 | 0.814435 | 2.666090e-51 | 5.081039e-49 |
MsaG001727 | MsaG028229 | 0.815516 | 1.538200e-51 | 3.012571e-49 |
MsaG002273 | MsaG028229 | 0.808844 | 4.322882e-50 | 7.179605e-48 |
MsaG003394 | MsaG028229 | 0.820721 | 1.036089e-52 | 2.322531e-50 |
MsaG003466 | MsaG028229 | 0.818303 | 3.667699e-52 | 7.715224e-50 |
MsaG003468 | MsaG028229 | 0.813966 | 3.379804e-51 | 6.365888e-49 |
MsaG004108 | MsaG028229 | 0.814503 | 2.574663e-51 | 4.915128e-49 |
MsaG004373 | MsaG028229 | 0.813395 | 4.507153e-51 | 8.368448e-49 |
MsaG005435 | MsaG028229 | 0.808151 | 6.067152e-50 | 9.909413e-48 |
MsaG005438 | MsaG028229 | 0.811794 | 1.005669e-50 | 1.794401e-48 |
MsaG006074 | MsaG028229 | 0.800379 | 2.477160e-48 | 3.378079e-46 |
MsaG006777 | MsaG028229 | 0.800215 | 2.674543e-48 | 3.633621e-46 |
MsaG007387 | MsaG028229 | 0.809713 | 2.821732e-50 | 4.785367e-48 |
MsaG008229 | MsaG028229 | 0.813606 | 4.053436e-51 | 7.565918e-49 |
MsaG008996 | MsaG028229 | 0.801940 | 1.191981e-48 | 1.683937e-46 |
MsaG010394 | MsaG028229 | 0.811940 | 9.350695e-51 | 1.674479e-48 |
MsaG011080 | MsaG028229 | 0.804421 | 3.674135e-49 | 5.495977e-47 |
MsaG011081 | MsaG028229 | 0.817755 | 4.870838e-52 | 1.010240e-49 |
MsaG011275 | MsaG028229 | 0.805949 | 1.765094e-49 | 2.735931e-47 |
MsaG012422 | MsaG028229 | 0.803539 | 5.593302e-49 | 8.197580e-47 |
MsaG013993 | MsaG028229 | 0.821119 | 8.399703e-53 | 1.902917e-50 |
MsaG014422 | MsaG028229 | 0.803499 | 5.701878e-49 | 8.348614e-47 |
MsaG014439 | MsaG028229 | 0.806221 | 1.548684e-49 | 2.416049e-47 |
MsaG014953 | MsaG028229 | 0.827643 | 2.495292e-54 | 6.755683e-52 |
MsaG015705 | MsaG028229 | 0.822202 | 4.731919e-53 | 1.103312e-50 |
MsaG016006 | MsaG028229 | 0.825107 | 9.959419e-54 | 2.512684e-51 |
MsaG016149 | MsaG028229 | 0.844572 | 1.302313e-58 | 5.883873e-56 |
MsaG016115 | MsaG028229 | 0.806150 | 1.602070e-49 | 2.495080e-47 |
MsaG017329 | MsaG028229 | 0.802310 | 1.001253e-48 | 1.426510e-46 |
MsaG017337 | MsaG028229 | 0.800396 | 2.458298e-48 | 3.353625e-46 |
MsaG017778 | MsaG028229 | 0.801546 | 1.434502e-48 | 2.008478e-46 |
MsaG018447 | MsaG028229 | 0.813455 | 4.373094e-51 | 8.131863e-49 |
MsaG019977 | MsaG028229 | 0.821166 | 8.193274e-53 | 1.858532e-50 |
MsaG020874 | MsaG028229 | 0.813224 | 4.913249e-51 | 9.083581e-49 |
MsaG020875 | MsaG028229 | 0.810361 | 2.048732e-50 | 3.529702e-48 |
MsaG021765 | MsaG028229 | 0.834524 | 5.189389e-56 | 1.713732e-53 |
MsaG023339 | MsaG028229 | 0.813199 | 4.975897e-51 | 9.193383e-49 |
MsaG023578 | MsaG028229 | 0.802951 | 7.391902e-49 | 1.068723e-46 |
MsaG024010 | MsaG028229 | 0.819151 | 2.359640e-52 | 5.074981e-50 |
MsaG026985 | MsaG028229 | 0.826466 | 4.755239e-54 | 1.245851e-51 |
MsaG027211 | MsaG028229 | 0.824018 | 1.792751e-53 | 4.390562e-51 |
MsaG028163 | MsaG028229 | 0.833840 | 7.684704e-56 | 2.486377e-53 |
MsaG028229 | MsaG028811 | 0.803643 | 5.323893e-49 | 7.821213e-47 |
MsaG028229 | MsaG029204 | 0.835710 | 2.614454e-56 | 8.945257e-54 |
MsaG028229 | MsaG029461 | 0.811873 | 9.664971e-51 | 1.727904e-48 |
MsaG028229 | MsaG029593 | 0.803976 | 4.543035e-49 | 6.725952e-47 |
MsaG028229 | MsaG030084 | 0.812645 | 6.570589e-51 | 1.197309e-48 |
MsaG028229 | MsaG032221 | 0.806274 | 1.509172e-49 | 2.357407e-47 |
MsaG028229 | MsaG033766 | 0.824103 | 1.712606e-53 | 4.203904e-51 |
MsaG028229 | MsaG034049 | 0.802462 | 9.318475e-49 | 1.332251e-46 |
MsaG028229 | MsaG035511 | 0.802668 | 8.451859e-49 | 1.214082e-46 |
MsaG028229 | MsaG040077 | 0.816733 | 8.251430e-52 | 1.666934e-49 |
MsaG028229 | MsaG040980 | 0.808673 | 4.701487e-50 | 7.776268e-48 |
MsaG028229 | MsaG041039 | 0.813588 | 4.089717e-51 | 7.630324e-49 |
MsaG028229 | MsaG041513 | 0.805530 | 2.159813e-49 | 3.314920e-47 |
MsaG028229 | MsaG042968 | 0.804114 | 4.254260e-49 | 6.318756e-47 |
MsaG028229 | MsaG043663 | 0.821056 | 8.683105e-53 | 1.963902e-50 |
MsaG028229 | MsaG044693 | 0.808075 | 6.298985e-50 | 1.026916e-47 |
MsaG028229 | MsaG045021 | 0.807674 | 7.656180e-50 | 1.236211e-47 |
MsaG028229 | MsaG045339 | 0.828593 | 1.477052e-54 | 4.107027e-52 |
MsaG028229 | MsaG045371 | 0.832726 | 1.452184e-55 | 4.547583e-53 |
MsaG028229 | MsaG045380 | 0.817815 | 4.723783e-52 | 9.812779e-50 |
MsaG028229 | MsaG045376 | 0.802403 | 9.580087e-49 | 1.367847e-46 |
MsaG028229 | MsaG045641 | 0.804977 | 2.816960e-49 | 4.268361e-47 |
MsaG028229 | MsaG046053 | 0.808566 | 4.954066e-50 | 8.172624e-48 |
MsaG028229 | MsaG046205 | 0.809220 | 3.595554e-50 | 6.025408e-48 |
MsaG028229 | MsaG046383 | 0.823070 | 2.978722e-53 | 7.110091e-51 |
MsaG028229 | MsaG046488 | 0.803751 | 5.058609e-49 | 7.450211e-47 |
MsaG028229 | MsaG046816 | 0.822053 | 5.123595e-53 | 1.189865e-50 |
MsaG028229 | MsaG047054 | 0.800288 | 2.585168e-48 | 3.517998e-46 |
MsaG028229 | MsaG007590 | 0.852247 | 1.002508e-60 | 5.874124e-58 |
MsaG028229 | MsaG008770 | 0.814115 | 3.133624e-51 | 5.924222e-49 |
MsaG028229 | MsaG010026 | 0.807423 | 8.649537e-50 | 1.388241e-47 |
MsaG028229 | MsaG011742 | 0.805012 | 2.769145e-49 | 4.199455e-47 |
MsaG028229 | MsaG013014 | 0.811291 | 1.292046e-50 | 2.277137e-48 |
MsaG028229 | MsaG020796 | 0.800380 | 2.476732e-48 | 3.377539e-46 |
MsaG028229 | MsaG018864 | 0.806881 | 1.125300e-49 | 1.783030e-47 |
MsaG028229 | MsaG031025 | 0.800072 | 2.859266e-48 | 3.872150e-46 |
MsaG028229 | MsaG037384 | 0.839265 | 3.240328e-57 | 1.236100e-54 |
MsaG028229 | MsaG035677 | 0.811928 | 9.407606e-51 | 1.684165e-48 |
MsaG028229 | MsaG036984 | 0.827032 | 3.490290e-54 | 9.288763e-52 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028229 | MtrunA17_Chr5g0435411 | 91.667 | 72 | 6 | 0 | 1 | 72 | 1 | 72 | 1.82e-44 | 142 |
MsaG028229 | MtrunA17_Chr5g0435401 | 75.556 | 90 | 22 | 0 | 93 | 182 | 177 | 266 | 1.39e-42 | 147 |
MsaG028229 | MtrunA17_Chr5g0435401 | 63.636 | 88 | 29 | 2 | 104 | 190 | 264 | 349 | 1.19e-24 | 99.4 |
MsaG028229 | MtrunA17_Chr5g0435431 | 87.500 | 72 | 9 | 0 | 1 | 72 | 1 | 72 | 9.05e-42 | 135 |
MsaG028229 | MtrunA17_Chr1g0192841 | 58.261 | 115 | 37 | 2 | 87 | 190 | 38 | 152 | 1.42e-31 | 114 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028229 | AT5G02220.1 | 50.000 | 60 | 28 | 1 | 8 | 67 | 12 | 69 | 4.05e-13 | 62.4 |
Find 40 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TTAGATTCCCTTTCCTTTAT+AGG | 0.104103 | 5:+59306596 | None:intergenic |
TTTGAAAATGGTTATGAAAT+TGG | 0.137150 | 5:-59306792 | None:intergenic |
CAGACTCTTCAGTGCTCTTT+TGG | 0.248679 | 5:+59304694 | None:intergenic |
TTATATCCACTCTGACCTAT+TGG | 0.347768 | 5:+59304995 | None:intergenic |
ATCCGACAACCGCTGTGTTT+TGG | 0.358584 | 5:+59306746 | None:intergenic |
AAAGGAAAGCAAGGAGATAA+AGG | 0.375471 | 5:-59304739 | MsaT028229.1:CDS |
TTCTTACTTAAAGCAGGAGT+TGG | 0.403219 | 5:-59305088 | MsaT028229.1:CDS |
GTGCTTATGCTGCAATACTT+TGG | 0.423903 | 5:-59305112 | MsaT028229.1:CDS |
TGGAGATTGAAGAAGGTTGC+CGG | 0.426728 | 5:-59306772 | MsaT028229.1:CDS |
GTTGTCAGACAAGGAGAAAC+AGG | 0.430794 | 5:-59304876 | MsaT028229.1:intron |
ATGAAATTGGAGATTGAAGA+AGG | 0.432769 | 5:-59306779 | MsaT028229.1:CDS |
TTTGGATTCTTACTTAAAGC+AGG | 0.455789 | 5:-59305094 | MsaT028229.1:CDS |
AAACGGAGACGTGCTCCAAT+AGG | 0.460049 | 5:-59305010 | MsaT028229.1:CDS |
TTTCATGTAGCCATATGAAA+AGG | 0.472708 | 5:-59304757 | MsaT028229.1:intron |
TCGTCTAAAAGCTGATCATC+AGG | 0.483966 | 5:-59304948 | MsaT028229.1:CDS |
GGTTTATTCAACGAAGAAGA+AGG | 0.495785 | 5:-59306678 | MsaT028229.1:CDS |
TTCTTCGTTGAATAAACCAC+CGG | 0.508962 | 5:+59306683 | None:intergenic |
CACCAAAACACAGCGGTTGT+CGG | 0.521171 | 5:-59306748 | MsaT028229.1:CDS |
ATAACAAAGAAAAGAAGAAA+CGG | 0.522686 | 5:-59305027 | MsaT028229.1:CDS |
GACGAGCACATTCCTGCTTG+AGG | 0.523607 | 5:+59304966 | None:intergenic |
TAGACATGGCTATTGATGCT+TGG | 0.537672 | 5:-59304904 | MsaT028229.1:CDS |
GAAGAAGAAGGCCCCACCGA+AGG | 0.545060 | 5:-59306666 | MsaT028229.1:CDS |
GGATTGAAGTAACCATCCTT+CGG | 0.555826 | 5:+59306650 | None:intergenic |
GCCATATGAAAAGGAAAGCA+AGG | 0.558682 | 5:-59304748 | MsaT028229.1:CDS |
GCCCGCGACTATGTGTCCCT+CGG | 0.570147 | 5:-59306720 | MsaT028229.1:CDS |
TGAAGTAACCATCCTTCGGT+GGG | 0.570885 | 5:+59306654 | None:intergenic |
GCCGAGGGACACATAGTCGC+GGG | 0.578000 | 5:+59306719 | None:intergenic |
AAGAAGGCCCCACCGAAGGA+TGG | 0.585344 | 5:-59306662 | MsaT028229.1:CDS |
ATATAATATATTCCTCAAGC+AGG | 0.589592 | 5:-59304978 | MsaT028229.1:CDS |
GCTGATCATCAGGACATATG+TGG | 0.590919 | 5:-59304938 | MsaT028229.1:CDS |
TTGAAGTAACCATCCTTCGG+TGG | 0.600524 | 5:+59306653 | None:intergenic |
TGGCAGAGAAATTGTAGACA+TGG | 0.605105 | 5:-59304918 | MsaT028229.1:CDS |
TGCCGAGGGACACATAGTCG+CGG | 0.615823 | 5:+59306718 | None:intergenic |
AAAATAAGCTCCAAATCAGG+GGG | 0.654554 | 5:+59306629 | None:intergenic |
TTGGAACAAGTTGTCAGACA+AGG | 0.660817 | 5:-59304885 | MsaT028229.1:CDS |
AGTAGCTCGTGTGAATTCAG+AGG | 0.669108 | 5:-59305134 | MsaT028229.1:CDS |
GAAGTAACCATCCTTCGGTG+GGG | 0.675290 | 5:+59306655 | None:intergenic |
CGTGCTCCAATAGGTCAGAG+TGG | 0.690376 | 5:-59305001 | MsaT028229.1:CDS |
GAGGGACACATAGTCGCGGG+CGG | 0.723461 | 5:+59306722 | None:intergenic |
TGCCGGACACCAAAACACAG+CGG | 0.804931 | 5:-59306755 | MsaT028229.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr5 | gene | 59304689 | 59306807 | 59304689 | ID=MsaG028229 |
Chr5 | mRNA | 59304689 | 59306807 | 59304689 | ID=MsaT028229.1;Parent=MsaG028229 |
Chr5 | exon | 59304689 | 59304769 | 59304689 | ID=MsaT028229.1.exon3;Parent=MsaT028229.1 |
Chr5 | CDS | 59304689 | 59304769 | 59304689 | ID=cds.MsaT028229.1;Parent=MsaT028229.1 |
Chr5 | exon | 59304877 | 59305162 | 59304877 | ID=MsaT028229.1.exon2;Parent=MsaT028229.1 |
Chr5 | CDS | 59304877 | 59305162 | 59304877 | ID=cds.MsaT028229.1;Parent=MsaT028229.1 |
Chr5 | exon | 59306596 | 59306807 | 59306596 | ID=MsaT028229.1.exon1;Parent=MsaT028229.1 |
Chr5 | CDS | 59306596 | 59306807 | 59306596 | ID=cds.MsaT028229.1;Parent=MsaT028229.1 |
Gene Sequence |
Protein sequence |