AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028230 MtrunA17_Chr5g0435401 85.238 420 33 5 1 400 1 411 0.0 712
MsaG028230 MtrunA17_Chr5g0445971 69.504 141 35 2 43 183 34 166 3.16e-57 185
MsaG028230 MtrunA17_Chr1g0192841 40.092 217 79 2 185 399 74 241 2.25e-43 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGATCTTCAGTGCTCTTT+TGG 0.157665 5:+59309940 None:intergenic
CACTAGTTTATCTCAATTAA+TGG 0.164628 5:-59311284 MsaT028230.1:CDS
ATTCAAATCACCTGGGAAAA+AGG 0.184616 5:+59309782 None:intergenic
AAAGCTGATCATCAGTTTAA+AGG 0.190474 5:-59310184 MsaT028230.1:CDS
CCAGTTGCAGTTTGAGTTGC+AGG 0.228640 5:+59311512 None:intergenic
TCGTAAGCTCACTGAGCTTT+TGG 0.297529 5:-59312607 MsaT028230.1:CDS
CCTCTATCAAACTACTCTTT+TGG 0.354206 5:-59309861 MsaT028230.1:CDS
TTATATCCACTCTGTCCTAT+TGG 0.357025 5:+59310576 None:intergenic
AGAGGAAAGCATGAAGATAA+AGG 0.358251 5:-59309985 MsaT028230.1:CDS
CGTTCGTTAGATTTGCCAAC+TGG 0.361646 5:-59309753 MsaT028230.1:CDS
CTCTCAGTGAGGATATAATA+TGG 0.364315 5:+59312636 None:intergenic
AGTTGTTTCTGCTCTAAAAC+TGG 0.365684 5:-59311624 MsaT028230.1:CDS
TTCATGCTTTCCTCTTCATA+TGG 0.373376 5:+59309993 None:intergenic
TTCACGCTTGCCTCTTCATA+TGG 0.385956 5:+59310328 None:intergenic
AGGTTGATCAAGAAAAGAAA+TGG 0.402611 5:-59311652 MsaT028230.1:CDS
AGAGGCAAGCGTGAAGATAA+AGG 0.407513 5:-59310320 MsaT028230.1:CDS
GTTGTCAGACAAGGAGAAAC+AGG 0.408256 5:-59310457 MsaT028230.1:intron
TTAATTTCATTAATGGAAGA+AGG 0.420089 5:-59312756 None:intergenic
GACTACTCTAGTCAAATTAC+AGG 0.434649 5:-59311223 MsaT028230.1:intron
CCTGCAACTCAAACTGCAAC+TGG 0.435997 5:-59311512 MsaT028230.1:CDS
CCAAAAGAGTAGTTTGATAG+AGG 0.436540 5:+59309861 None:intergenic
TCGTGACCTCCAAGTAAAAC+AGG 0.439872 5:+59312057 None:intergenic
CGATAATCATTCAAATCACC+TGG 0.440231 5:+59309774 None:intergenic
TTGAGACATTGTACTTCTAC+AGG 0.444314 5:-59311538 MsaT028230.1:CDS
ACTGCAACTGGCAGCAATAC+AGG 0.446573 5:-59311500 MsaT028230.1:intron
AGGAAAGGAATTAGTAGTGA+TGG 0.453771 5:-59312736 MsaT028230.1:CDS
AGAACAAAGAAAAGAAGAAA+CGG 0.457756 5:-59310608 MsaT028230.1:intron
TACTTGGAGGTCACGAAAAG+AGG 0.458176 5:-59312050 MsaT028230.1:CDS
TAGTAGTCACCACACACAAT+AGG 0.458403 5:+59309900 None:intergenic
AAACGGAGAGGTGCTCCAAT+AGG 0.474962 5:-59310591 MsaT028230.1:CDS
TTCATTAATGGAAGAAGGAA+AGG 0.479210 5:-59312751 None:intergenic
TATGCACTCTTCTGTCCCCT+TGG 0.481011 5:+59310241 None:intergenic
TTCATCAATGGAAGAAGCAA+AGG 0.491657 5:-59312679 MsaT028230.1:CDS
GGTTGATCAAGAAAAGAAAT+GGG 0.491923 5:-59311651 MsaT028230.1:CDS
CTTTATAAGGTCCAGTTGAA+AGG 0.507012 5:-59311321 MsaT028230.1:intron
AGAAGCAAAGGAATTAGAGA+TGG 0.514965 5:-59312667 MsaT028230.1:CDS
AGTAAAACAGGTACAAGTCA+AGG 0.532503 5:+59312069 None:intergenic
AAATCACGGCTATTGATGCT+TGG 0.536227 5:-59310153 MsaT028230.1:CDS
TAGAGATGGCTATTGATGCT+TGG 0.538363 5:-59310485 MsaT028230.1:CDS
TCGACTTAAAGCTTATCATC+AGG 0.545141 5:-59310529 MsaT028230.1:CDS
CGACAACAAGTATAGTCACT+CGG 0.548231 5:+59309725 None:intergenic
GTCTAGCGCATTCATGCTTG+AGG 0.557923 5:+59310209 None:intergenic
GTTCGTTAGATTTGCCAACT+GGG 0.558967 5:-59309752 MsaT028230.1:CDS
AACATGAGATTGAATCCAGA+AGG 0.564550 5:+59312582 None:intergenic
GTTTCTGCTCTAAAACTGGA+AGG 0.575106 5:-59311620 MsaT028230.1:CDS
TTTCATGTAGCCATATGAAG+AGG 0.580743 5:-59310338 MsaT028230.1:intron
AGTCACTCGGGTTCCCCAGT+TGG 0.582592 5:+59309738 None:intergenic
ATATAATATATTCCTCAAGC+AGG 0.589592 5:-59310559 MsaT028230.1:CDS
GTCGAGCACACTCCTGCTTG+AGG 0.592986 5:+59310547 None:intergenic
AAAGAAAAGAAGAAACATAG+AGG 0.596397 5:-59310268 MsaT028230.1:CDS
GAAACATAGAGGTGTTCCAA+GGG 0.597227 5:-59310257 MsaT028230.1:CDS
AGAAACATAGAGGTGTTCCA+AGG 0.610697 5:-59310258 MsaT028230.1:CDS
GTAAAACAGGTACAAGTCAA+GGG 0.610799 5:+59312070 None:intergenic
TTTCATGTAGCCATATGAAG+AGG 0.611306 5:-59310003 MsaT028230.1:intron
TTCGTTAGATTTGCCAACTG+GGG 0.615840 5:-59309751 MsaT028230.1:CDS
GCTTATCATCAGGACGTAGG+TGG 0.622815 5:-59310519 MsaT028230.1:CDS
GGAATTAGTAGTGATGGAGA+TGG 0.631774 5:-59312730 MsaT028230.1:CDS
GACAACAAGTATAGTCACTC+GGG 0.642555 5:+59309726 None:intergenic
TTGGAGGTCACGAAAAGAGG+AGG 0.644365 5:-59312047 MsaT028230.1:CDS
GATAATCATTCAAATCACCT+GGG 0.650646 5:+59309775 None:intergenic
GGACGAGCAAGATTATCAGA+AGG 0.655330 5:-59311263 MsaT028230.1:CDS
TTGGAATAAGTTGTCAGACA+AGG 0.660735 5:-59310466 MsaT028230.1:CDS
AAAGCTCAGTGAGCTTACGA+TGG 0.661623 5:+59312610 None:intergenic
ACATGAGATTGAATCCAGAA+GGG 0.670630 5:+59312583 None:intergenic
AAAGAAAAGAAGAAACGGAG+AGG 0.672313 5:-59310603 MsaT028230.1:intron
AATTTGACTAGAGTAGTCCT+CGG 0.673968 5:+59311228 None:intergenic
AAAGCTTATCATCAGGACGT+AGG 0.684875 5:-59310522 MsaT028230.1:CDS
GGTGCTCCAATAGGACAGAG+TGG 0.688534 5:-59310582 MsaT028230.1:CDS
CATTAATTGAGATAAACTAG+TGG 0.690114 5:+59311285 None:intergenic
GAAGGCAGCAAAGATATCCG+AGG 0.700751 5:-59311245 MsaT028230.1:CDS
GAAAAGAGGAGGTTATCACC+AGG 0.701758 5:-59312036 MsaT028230.1:intron
GTTGATCAAGAAAAGAAATG+GGG 0.707030 5:-59311650 MsaT028230.1:CDS
AAACATAGAGGTGTTCCAAG+GGG 0.716007 5:-59310256 MsaT028230.1:CDS
TACGATGGAAGCTCTCAGTG+AGG 0.727693 5:+59312625 None:intergenic
GAAGAGAGACCTATTGTGTG+TGG 0.729317 5:-59309909 MsaT028230.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 59309740 59312766 59309740 ID=MsaG028230
Chr5 mRNA 59309740 59312766 59309740 ID=MsaT028230.1;Parent=MsaG028230
Chr5 exon 59309740 59310015 59309740 ID=MsaT028230.1.exon7;Parent=MsaT028230.1
Chr5 CDS 59309740 59310015 59309740 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59310126 59310350 59310126 ID=MsaT028230.1.exon6;Parent=MsaT028230.1
Chr5 CDS 59310126 59310350 59310126 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59310458 59310621 59310458 ID=MsaT028230.1.exon5;Parent=MsaT028230.1
Chr5 CDS 59310458 59310621 59310458 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59311224 59311334 59311224 ID=MsaT028230.1.exon4;Parent=MsaT028230.1
Chr5 CDS 59311224 59311334 59311224 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59311501 59311672 59311501 ID=MsaT028230.1.exon3;Parent=MsaT028230.1
Chr5 CDS 59311501 59311672 59311501 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59312037 59312112 59312037 ID=MsaT028230.1.exon2;Parent=MsaT028230.1
Chr5 CDS 59312037 59312112 59312037 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Chr5 exon 59312585 59312766 59312585 ID=MsaT028230.1.exon1;Parent=MsaT028230.1
Chr5 CDS 59312585 59312766 59312585 ID=cds.MsaT028230.1;Parent=MsaT028230.1
Gene Sequence

>MsaT028230.1

ATGGAAGAAGGAAAGGAATTAGTAGTGATGGAGATGGTTCCTTATGAAAATGAAAATGAGAATTTTTCATCAATGGAAGAAGCAAAGGAATTAGAGATGGAGATGATTCCATATTATATCCTCACTGAGAGCTTCCATCGTAAGCTCACTGAGCTTTTGGACCCTTCTGGATTCAATCTCATATTTGATGTTCGAAAAACATCCCTTGACTTGTACCTGTTTTACTTGGAGGTCACGAAAAGAGGAGGTTATCACCAGGTTGATCAAGAAAAGAAATGGGGTGAAGTTGTTTCTGCTCTAAAACTGGAAGGAAACAATGCAACCTTATGTGATCAACTTGAAAAGCTATATAAAGAGCTTCTGTACAAATTTGAGACATTGTACTTCTACAGGTCTCCTGCAACTCAAACTGCAACTGGCAGCAATACAGGTCCAGTTGAAAGGAATCAAAACTCAACCACTAGTTTATCTCAATTAATGGACGAGCAAGATTATCAGAAGGCAGCAAAGATATCCGAGGACTACTCTAGTCAAATTACAGAAAAGAAGAAACGGAGAGGTGCTCCAATAGGACAGAGTGGATATAATATATTCCTCAAGCAGGAGTGTGCTCGACTTAAAGCTTATCATCAGGACGTAGGTGGAAGAAAAATCATAGAGATGGCTATTGATGCTTGGAATAAGTTGTCAGACAAGGAGAAACAGCCATATGAAGAGGCAAGCGTGAAGATAAAGGAAGAAATTAAAGAAGCACCCACCAATAACAAAGAAAAGAAGAAACATAGAGGTGTTCCAAGGGGACAGAAGAGTGCATATCAAATATTCCTCAAGCATGAATGCGCTAGACTTAAAGCTGATCATCAGTTTAAAGGTGACCGAAAAATCACGGCTATTGATGCTTGGAAGATGATGTCCCCCATGGAAAAACTGCCATATGAAGAGGAAAGCATGAAGATAAAGGAAGAAATTAAAGAAGCAATGATCCAAAAGAGCACTGAAGATCTTGAGAGAGATGAAGAGAGACCTATTGTGTGTGGTGACTACTACTCTGTAACTTCACAACCTCTATCAAACTACTCTTTTGGCAACAACGCAGCGGTCGACTTAGATTTTAAACTGACAGAAAAAACATCCGAGGATCCCTTTTTCCCAGGTGATTTGAATGATTATCGTTCGTTAGATTTGCCAACTGGGGAACCCGAGTGA

Protein sequence

>MsaT028230.1

MEEGKELVVMEMVPYENENENFSSMEEAKELEMEMIPYYILTESFHRKLTELLDPSGFNLIFDVRKTSLDLYLFYLEVTKRGGYHQVDQEKKWGEVVSALKLEGNNATLCDQLEKLYKELLYKFETLYFYRSPATQTATGSNTGPVERNQNSTTSLSQLMDEQDYQKAAKISEDYSSQITEKKKRRGAPIGQSGYNIFLKQECARLKAYHQDVGGRKIIEMAIDAWNKLSDKEKQPYEEASVKIKEEIKEAPTNNKEKKKHRGVPRGQKSAYQIFLKHECARLKADHQFKGDRKITAIDAWKMMSPMEKLPYEEESMKIKEEIKEAMIQKSTEDLERDEERPIVCGDYYSVTSQPLSNYSFGNNAAVDLDFKLTEKTSEDPFFPGDLNDYRSLDLPTGEPE*