AlfalfaGEDB | MsaG028248

M. sativa ssp. caerulea / MsaG028248

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028248	RHN65210.1	48.387	217	81	4	13	201	252	465	7.59e-55	192
MsaG028248	RHN65210.1	66.667	219	60	2	2	208	11	228	5.47e-90	283

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028248	sp\|Q8L3W1\|VRN1_ARATH	41.667	84	49	0	13	96	21	104	3.02e-17	82.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028248	tr\|A0A396IJ58\|A0A396IJ58_MEDTR	48.387	217	81	4	13	201	252	465	3.63e-55	192
MsaG028248	tr\|A0A396IJ58\|A0A396IJ58_MEDTR	66.667	219	60	2	2	208	11	228	2.61e-90	283

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TFs/TRs:

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Gene ID	Type	Classification
MsaG028248	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000015	MsaG028248	0.823063	2.990066e-53	7.135776e-51
MsaG000030	MsaG028248	0.800394	2.459617e-48	3.355326e-46
MsaG000045	MsaG028248	0.812015	9.007456e-51	1.615925e-48
MsaG000071	MsaG028248	0.849608	5.509811e-60	2.946968e-57
MsaG000083	MsaG028248	0.830751	4.433830e-55	1.311001e-52
MsaG000113	MsaG028248	0.822657	3.713911e-53	8.766403e-51
MsaG000115	MsaG028248	0.803565	5.524150e-49	8.100936e-47
MsaG000135	MsaG028248	0.859349	8.624651e-63	6.541666e-60
MsaG000151	MsaG028248	0.870359	3.153627e-66	3.709906e-63
MsaG000153	MsaG028248	0.840120	1.945543e-57	7.623472e-55

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028248	MtrunA17_Chr3g0077461	66.667	219	60	2	2	208	11	228	5.04e-94	283
MsaG028248	MtrunA17_Chr3g0077461	48.387	217	81	4	13	201	252	465	6.98e-59	192
MsaG028248	MtrunA17_Chr3g0077411	63.721	215	58	2	13	208	40	253	5.33e-87	266
MsaG028248	MtrunA17_Chr3g0077411	45.188	239	81	4	13	202	277	514	5.64e-59	194
MsaG028248	MtrunA17_Chr3g0077361	64.929	211	54	2	13	203	55	265	1.17e-81	252
MsaG028248	MtrunA17_Chr3g0077361	47.449	196	96	2	13	202	304	498	2.20e-46	160
MsaG028248	MtrunA17_Chr5g0429931	51.867	241	74	2	11	210	8	247	1.17e-77	234
MsaG028248	MtrunA17_Chr4g0009491	62.944	197	54	1	9	186	8	204	6.52e-77	238
MsaG028248	MtrunA17_Chr4g0009491	48.925	186	54	1	13	157	240	425	1.07e-49	167
MsaG028248	MtrunA17_Chr3g0077451	54.054	222	69	3	13	202	11	231	1.77e-72	220

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028248	AT3G18990.1	41.667	84	49	0	13	96	21	104	3.07e-18	82.4
MsaG028248	AT3G18990.2	41.667	84	49	0	13	96	21	104	6.81e-18	81.6
MsaG028248	AT1G49475.1	38.542	96	58	1	11	106	47	141	1.81e-16	75.1
MsaG028248	AT4G01580.1	34.737	95	62	0	4	98	36	130	1.27e-15	72.8
MsaG028248	AT3G18960.2	33.684	95	63	0	4	98	36	130	3.18e-15	71.6
MsaG028248	AT3G18960.1	33.684	95	63	0	4	98	36	130	7.71e-15	70.9
MsaG028248	AT3G18960.3	35.632	87	56	0	12	98	7	93	2.11e-14	68.9

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Find 42 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTCAAGGAAAGCAG+TGG	0.599450	5:-59544488	MsaT028248.1:CDS
AAACTCATACATAACTCCAT+CGG	0.639008	5:+59544377	None:intergenic
AAATCAATGCGAATAACGTT+TGG	0.379436	5:-59544432	MsaT028248.1:CDS
AAATTCTCAAGGAAAGCAGT+GGG	0.637805	5:-59544487	MsaT028248.1:CDS
AAGGATTGGGAACGAAAAGA+AGG	0.522583	5:+59544680	None:intergenic
AATCTTGTTATAAACTCATC+TGG	0.413404	5:+59544971	None:intergenic
ACAAGTTACAGCTTCTCACA+CGG	0.646742	5:-59544322	MsaT028248.1:CDS
ACCATCAGGAACAGTAATCG+TGG	0.675565	5:+59544925	None:intergenic
ACTGCTTTCCTTGAGAATTT+TGG	0.150373	5:+59544490	None:intergenic
AGGATTGGGAACGAAAAGAA+GGG	0.605751	5:+59544681	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	59544260	59545035	59544260	ID=MsaG028248
Chr5	mRNA	59544260	59545035	59544260	ID=MsaT028248.1;Parent=MsaG028248
Chr5	exon	59544260	59544568	59544260	ID=MsaT028248.1.exon2;Parent=MsaT028248.1
Chr5	CDS	59544260	59544568	59544260	ID=cds.MsaT028248.1;Parent=MsaT028248.1
Chr5	exon	59544664	59545035	59544664	ID=MsaT028248.1.exon1;Parent=MsaT028248.1
Chr5	CDS	59544664	59545035	59544664	ID=cds.MsaT028248.1;Parent=MsaT028248.1

Gene Sequence

>MsaT028248.1

ATGTATCACCCTTCCTCTAAAGATTGGTCACTACAGAGAATTCCAGATGAGTTTATAACAAGATTTGGGAATGAACTCAACAATGTTGCCACGATTACTGTTCCTGATGGTTGTGAATGGCATATGGAAGTAAAGAAACGTGGCAACGAAGTTTTCTTTTGCAAAAATTGGCAACCGTTCGCAGAACACTACTCTATCTGTTATGGTTGTTATGTAGATTTCAAGTATGAAGGGAATTCAAAGTTTAATGTTGTTATATATGATACAAATTCTGTCGAGATTTCCTATCCATTCAAAACTGGGAAGACTAATGGAGAACCAAACCCAAAAAACCCTTCTTTTCGTTCCCAATCCTTTGACTGTCGATATGCGTATGTTCCTGCTGAATTCGCTGCAAAGTATTTGAAACCAAATGTTCTGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGAATAAAAGCGGTGGAAAATCAATGCGAATAACGTTTGGATTCTTTAGATTTGCAAGGGAAAACAATCTATCCGATGGAGTTATGTATGAGTTTGAGCTTATCAAGAGGAAGCCGCGTGTTGTGTTACAAGTTACAGCTTCTCACACGGTAAAGAGCTGTTTCACATTCGTTTATGCGTCAGTTCTATTTTCTTACAATTTTTTCTTCTAA

Protein sequence

>MsaT028248.1

MYHPSSKDWSLQRIPDEFITRFGNELNNVATITVPDGCEWHMEVKKRGNEVFFCKNWQPFAEHYSICYGCYVDFKYEGNSKFNVVIYDTNSVEISYPFKTGKTNGEPNPKNPSFRSQSFDCRYAYVPAEFAAKYLKPNVLFKLQNSQGKQWEVYCVLNKSGGKSMRITFGFFRFARENNLSDGVMYEFELIKRKPRVVLQVTASHTVKSCFTFVYASVLFSYNFFF*