AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028253


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028253 MtrunA17_Chr5g0435131 94.798 173 3 2 1 173 1 167 4.03e-116 326
MsaG028253 MtrunA17_Chr4g0025901 46.471 170 81 4 6 168 4 170 2.30e-39 132
MsaG028253 MtrunA17_Chr4g0025911 40.828 169 93 4 3 168 1 165 7.91e-33 115
MsaG028253 MtrunA17_Chr1g0177881 59.420 69 28 0 97 165 102 170 7.39e-24 92.4
MsaG028253 MtrunA17_Chr7g0267111 36.842 171 93 4 6 161 4 174 1.43e-23 92.0
MsaG028253 MtrunA17_Chr8g0390531 44.304 79 44 0 88 166 249 327 3.88e-18 80.1
MsaG028253 MtrunA17_Chr3g0141471 50.725 69 34 0 94 162 195 263 7.72e-13 65.1
MsaG028253 MtrunA17_Chr5g0432531 43.284 67 38 0 88 154 177 243 5.99e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028253 AT3G52525.1 60.317 63 25 0 98 160 67 129 3.22e-21 85.5
MsaG028253 AT5G19650.1 53.030 66 31 0 97 162 154 219 6.98e-21 85.9
MsaG028253 AT2G36026.1 60.656 61 24 0 102 162 91 151 7.06e-20 82.4
MsaG028253 AT2G18500.1 48.387 62 32 0 95 156 224 285 8.09e-17 76.6
MsaG028253 AT5G22240.1 45.205 73 40 0 91 163 90 162 1.61e-15 71.2
MsaG028253 AT1G06920.1 40.580 69 41 0 94 162 240 308 6.95e-13 65.9

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGGAGAAGTGAGGGTATT+AGG 0.156150 5:-59592472 None:intergenic
CCATCACTACAAAGCTTCTT+AGG 0.242237 5:-59592817 None:intergenic
GGGTATTAGGGTTTGTGTTT+TGG 0.265400 5:-59592459 None:intergenic
CTCTATACTTCAAATGATAT+TGG 0.278958 5:+59592672 MsaT028253.1:CDS
TGGGAGAAGTGAGGGTATTA+GGG 0.326639 5:-59592471 None:intergenic
TGTCATTGTTGAAGCGTTCA+TGG 0.333768 5:+59592774 MsaT028253.1:CDS
ACAACAACAATAATAATTAT+AGG 0.352089 5:+59592569 MsaT028253.1:CDS
TTTGTCGTTGGTGTTGGCTT+CGG 0.396392 5:-59592509 None:intergenic
AGAGGGTTCAAGTACGTTAG+AGG 0.399454 5:-59592413 None:intergenic
TTCGGCGTCGTGGGAAGTGG+TGG 0.442026 5:-59592491 None:intergenic
GGCTTCGGCGTCGTGGGAAG+TGG 0.446154 5:-59592494 None:intergenic
GGTGTTGGCTTCGGCGTCGT+GGG 0.479777 5:-59592500 None:intergenic
CCTAAGAAGCTTTGTAGTGA+TGG 0.489027 5:+59592817 MsaT028253.1:CDS
GGGAAGTGGTGGGAGAAGTG+AGG 0.511341 5:-59592480 None:intergenic
GAAATCTTTGTCGTTGGTGT+TGG 0.511751 5:-59592515 None:intergenic
GGAAGTGGTGGGAGAAGTGA+GGG 0.519603 5:-59592479 None:intergenic
TGGTGTTGGCTTCGGCGTCG+TGG 0.520114 5:-59592501 None:intergenic
TTCTCATCTAACAAAAGTTG+TGG 0.528866 5:+59592373 MsaT028253.1:CDS
TCGGCGTCGTGGGAAGTGGT+GGG 0.541073 5:-59592490 None:intergenic
GAGTTTCGAAAATCTTCATA+CGG 0.557400 5:-59592652 None:intergenic
AGATAGCATTGCCGTTGAGA+AGG 0.559747 5:+59592618 MsaT028253.1:CDS
GAGGGTTCAAGTACGTTAGA+GGG 0.563551 5:-59592412 None:intergenic
GCCGTTGTCATTCGAGATGG+TGG 0.579315 5:-59592545 None:intergenic
GTTGCCGTTGTCATTCGAGA+TGG 0.580061 5:-59592548 None:intergenic
GTTGTCATTCGAGATGGTGG+TGG 0.580502 5:-59592542 None:intergenic
TGCCGTTGAGAAGGAATCGA+AGG 0.586299 5:+59592627 MsaT028253.1:CDS
GACGTGTGAAGGACATGTGA+AGG 0.602789 5:+59592842 MsaT028253.1:CDS
GGAAGTGAAATCTTTGTCGT+TGG 0.605510 5:-59592521 None:intergenic
CTCCTTCGATTCCTTCTCAA+CGG 0.636372 5:-59592629 None:intergenic
ACCACCATCTCGAATGACAA+CGG 0.639408 5:+59592544 MsaT028253.1:CDS
ACGCTTCAACAATGACATGA+TGG 0.645246 5:-59592768 None:intergenic
TAGTGATGGTAGACGTGTGA+AGG 0.668871 5:+59592831 MsaT028253.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 59592331 59592861 59592331 ID=MsaG028253
Chr5 mRNA 59592331 59592861 59592331 ID=MsaT028253.1;Parent=MsaG028253
Chr5 exon 59592331 59592861 59592331 ID=MsaT028253.1.exon1;Parent=MsaT028253.1
Chr5 CDS 59592331 59592861 59592331 ID=cds.MsaT028253.1;Parent=MsaT028253.1
Gene Sequence

>MsaT028253.1

ATGTCTTTCAATTTCAACAAGAGAAGCCTCGTCAAAACCCTTTTCTCATCTAACAAAAGTTGTGGTTGCATCAAAATAAAACCCTCTAACGTACTTGAACCCTCTCAAAAACCTAAAATATCCTTTAACCAAAACACAAACCCTAATACCCTCACTTCTCCCACCACTTCCCACGACGCCGAAGCCAACACCAACGACAAAGATTTCACTTCCACCACCATCTCGAATGACAACGGCAACAACAACAATAATAATTATAGGATTTTAAAGCCGGGTACAAAACTCATAGATAGCATTGCCGTTGAGAAGGAATCGAAGGAGCCGTATGAAGATTTTCGAAACTCTATACTTCAAATGATATTGGAGAGAGAGATTTATTCAGAAAATGATCTTCAAGAATTACTTGAGTGTTTTCTTCAACTTAATGCAAAGTGTCACCATCATGTCATTGTTGAAGCGTTCATGGAGACATGTGAAGAAATATTTCCTAAGAAGCTTTGTAGTGATGGTAGACGTGTGAAGGACATGTGA

Protein sequence

>MsaT028253.1

MSFNFNKRSLVKTLFSSNKSCGCIKIKPSNVLEPSQKPKISFNQNTNPNTLTSPTTSHDAEANTNDKDFTSTTISNDNGNNNNNNYRILKPGTKLIDSIAVEKESKEPYEDFRNSILQMILEREIYSENDLQELLECFLQLNAKCHHHVIVEAFMETCEEIFPKKLCSDGRRVKDM*