AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028376 MtrunA17_Chr5g0433781 90.508 590 49 1 1 590 62 644 0.0 1121
MsaG028376 MtrunA17_Chr3g0109821 60.621 612 212 18 1 589 62 667 0.0 685
MsaG028376 MtrunA17_Chr6g0481091 73.469 98 26 0 2 99 63 160 4.10e-48 168
MsaG028376 MtrunA17_Chr2g0309891 71.154 104 29 1 1 103 62 165 5.97e-45 162
MsaG028376 MtrunA17_Chr1g0201021 62.617 107 39 1 2 108 76 181 2.95e-36 143
MsaG028376 MtrunA17_Chr4g0017921 46.923 130 64 2 2 131 66 190 4.22e-36 138
MsaG028376 MtrunA17_Chr5g0400461 64.706 102 26 2 1 95 62 160 7.60e-36 137
MsaG028376 MtrunA17_Chr5g0428991 63.830 94 34 0 2 95 82 175 7.03e-35 139
MsaG028376 MtrunA17_Chr3g0092671 59.259 108 42 2 1 108 62 167 5.70e-34 135
MsaG028376 MtrunA17_Chr3g0128151 63.158 95 35 0 2 96 79 173 6.31e-34 136
MsaG028376 MtrunA17_Chr3g0128111 60.000 95 38 0 2 96 99 193 1.04e-33 129
MsaG028376 MtrunA17_Chr8g0372121 45.763 118 62 1 2 119 64 179 2.72e-33 129
MsaG028376 MtrunA17_Chr8g0359921 58.163 98 39 1 2 97 69 166 3.00e-33 129
MsaG028376 MtrunA17_Chr2g0318081 56.250 96 42 0 2 97 71 166 3.80e-33 130
MsaG028376 MtrunA17_Chr4g0054791 60.204 98 36 1 1 95 62 159 4.28e-33 129
MsaG028376 MtrunA17_Chr4g0042561 56.122 98 39 1 2 95 64 161 6.82e-33 127
MsaG028376 MtrunA17_Chr1g0197361 57.407 108 44 2 1 108 65 170 7.90e-33 133
MsaG028376 MtrunA17_Chr8g0364791 51.852 108 51 1 1 108 62 168 1.29e-32 130
MsaG028376 MtrunA17_Chr6g0484441 60.000 95 36 1 2 96 60 152 2.45e-32 127
MsaG028376 MtrunA17_Chr8g0384721 45.205 146 60 5 2 128 62 206 3.47e-32 125
MsaG028376 MtrunA17_Chr1g0200561 53.125 96 43 1 2 97 77 170 1.64e-31 125
MsaG028376 MtrunA17_Chr4g0018391 44.444 117 63 2 2 118 65 179 2.52e-31 124
MsaG028376 MtrunA17_Chr3g0124761 57.426 101 39 3 2 99 62 161 3.20e-31 123
MsaG028376 MtrunA17_Chr5g0405211 51.546 97 45 1 2 98 66 160 3.85e-31 124
MsaG028376 MtrunA17_Chr6g0461661 52.830 106 46 2 2 103 73 178 4.39e-31 124
MsaG028376 MtrunA17_Chr4g0056541 50.980 102 48 1 2 103 65 164 4.43e-31 124
MsaG028376 MtrunA17_Chr4g0060931 55.556 99 42 2 2 98 72 170 5.11e-31 124
MsaG028376 MtrunA17_Chr2g0309331 53.684 95 41 2 2 95 68 160 5.22e-31 124
MsaG028376 MtrunA17_Chr6g0453451 54.808 104 35 2 2 98 75 173 1.01e-30 122
MsaG028376 MtrunA17_Chr5g0417791 52.475 101 44 1 2 98 58 158 1.07e-30 120
MsaG028376 MtrunA17_Chr5g0398971 54.737 95 41 2 2 95 65 158 1.48e-30 122
MsaG028376 MtrunA17_Chr3g0091921 56.701 97 40 2 2 96 71 167 1.79e-30 121
MsaG028376 MtrunA17_Chr2g0318101 54.630 108 36 3 2 96 70 177 3.38e-30 121
MsaG028376 MtrunA17_Chr7g0252971 50.000 112 43 2 2 100 63 174 3.66e-30 121
MsaG028376 MtrunA17_Chr3g0139331 56.701 97 38 2 2 96 66 160 4.25e-30 121
MsaG028376 MtrunA17_Chr3g0130731 51.724 116 45 4 2 107 62 176 4.78e-30 119
MsaG028376 MtrunA17_Chr8g0346001 47.573 103 45 1 2 95 73 175 6.77e-30 122
MsaG028376 MtrunA17_Chr3g0130101 48.148 108 55 1 1 108 63 169 8.31e-30 120
MsaG028376 MtrunA17_Chr8g0384201 47.222 108 56 1 1 108 62 168 9.07e-30 122
MsaG028376 MtrunA17_Chr3g0113001 41.615 161 74 3 2 159 20 163 9.73e-30 119
MsaG028376 MtrunA17_Chr2g0316971 58.586 99 37 2 2 96 78 176 1.58e-29 120
MsaG028376 MtrunA17_Chr7g0214071 57.292 96 37 3 2 95 67 160 1.93e-29 117
MsaG028376 MtrunA17_Chr1g0147421 56.701 97 38 2 2 96 83 177 5.57e-29 118
MsaG028376 MtrunA17_Chr7g0253011 52.885 104 43 3 2 99 72 175 1.32e-28 117
MsaG028376 MtrunA17_Chr2g0312321 50.000 110 51 2 2 107 64 173 2.59e-28 115
MsaG028376 MtrunA17_Chr7g0217721 65.278 72 25 0 2 73 74 145 4.44e-28 115
MsaG028376 MtrunA17_Chr4g0076731 55.102 98 41 2 2 98 109 204 4.72e-28 117
MsaG028376 MtrunA17_Chr1g0183741 58.763 97 34 3 2 96 65 157 7.24e-28 113
MsaG028376 MtrunA17_Chr2g0321741 54.945 91 34 2 2 92 48 131 8.84e-28 114
MsaG028376 MtrunA17_Chr1g0197351 54.867 113 44 3 2 108 99 210 9.63e-28 117
MsaG028376 MtrunA17_Chr7g0217711 63.889 72 26 0 2 73 74 145 1.00e-27 109
MsaG028376 MtrunA17_Chr4g0047841 48.333 120 37 3 2 96 70 189 1.01e-27 114
MsaG028376 MtrunA17_Chr6g0453131 47.619 105 45 2 2 96 70 174 2.24e-27 114
MsaG028376 MtrunA17_Chr7g0263931 52.000 100 42 3 2 95 66 165 3.37e-27 112
MsaG028376 MtrunA17_Chr7g0261411 59.574 94 32 3 2 93 65 154 4.10e-27 111
MsaG028376 MtrunA17_Chr2g0326361 53.000 100 41 3 2 95 69 168 9.17e-27 111
MsaG028376 MtrunA17_Chr3g0144271 68.493 73 21 1 2 72 79 151 1.99e-26 112
MsaG028376 MtrunA17_Chr4g0038751 48.515 101 44 3 2 99 70 165 3.10e-26 108
MsaG028376 MtrunA17_Chr3g0113031 38.312 154 74 2 10 159 3 139 4.92e-26 107
MsaG028376 MtrunA17_Chr8g0361731 44.340 106 48 2 2 96 69 174 9.30e-26 109
MsaG028376 MtrunA17_Chr5g0417001 44.762 105 48 2 2 96 69 173 2.45e-25 108
MsaG028376 MtrunA17_Chr7g0243371 49.000 100 49 2 2 99 19 118 5.34e-25 100
MsaG028376 MtrunA17_Chr7g0266701 44.231 104 52 3 2 99 65 168 1.66e-21 94.4
MsaG028376 MtrunA17_Chr2g0311661 35.294 187 94 7 2 170 77 254 2.16e-21 94.7
MsaG028376 MtrunA17_Chr8g0385991 39.370 127 63 4 2 120 67 187 9.38e-21 91.3
MsaG028376 MtrunA17_Chr4g0026141 44.211 95 49 2 2 92 65 159 1.49e-20 91.7
MsaG028376 MtrunA17_Chr8g0345671 47.872 94 41 3 2 93 69 156 2.39e-20 93.6
MsaG028376 MtrunA17_Chr2g0311651 45.045 111 46 3 2 97 78 188 4.94e-20 91.3
MsaG028376 MtrunA17_Chr2g0321631 43.158 95 50 2 2 92 65 159 6.07e-20 89.7
MsaG028376 MtrunA17_Chr3g0131911 45.098 102 46 2 4 95 69 170 2.43e-19 87.8
MsaG028376 MtrunA17_Chr5g0442771 44.340 106 47 3 2 95 73 178 3.53e-19 88.6
MsaG028376 MtrunA17_Chr8g0345691 43.617 94 45 3 2 93 70 157 3.97e-18 87.0
MsaG028376 MtrunA17_Chr8g0345741 46.479 71 35 2 2 70 49 118 1.64e-15 74.3
MsaG028376 MtrunA17_Chr8g0345711 50.000 72 31 3 2 70 70 139 3.29e-15 73.9
MsaG028376 MtrunA17_Chr8g0345721 45.070 71 36 2 2 70 122 191 4.64e-15 74.3
MsaG028376 MtrunA17_Chr8g0345731 40.404 99 46 4 2 95 82 172 6.34e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028376 AT1G65910.1 40.037 542 248 20 1 519 62 549 5.94e-106 332
MsaG028376 AT5G17260.1 35.031 491 207 23 2 484 63 449 4.06e-65 221
MsaG028376 AT3G03200.1 33.267 505 224 22 2 496 63 464 4.84e-65 221
MsaG028376 AT3G17730.1 72.449 98 27 0 2 99 63 160 4.23e-48 168
MsaG028376 AT1G54330.1 67.619 105 30 2 1 105 62 162 1.83e-38 144
MsaG028376 AT3G10500.1 43.478 184 92 3 1 173 65 247 6.87e-37 145
MsaG028376 AT5G04410.1 60.185 108 42 1 1 108 65 171 9.03e-37 145
MsaG028376 AT4G17980.1 61.616 99 34 2 1 95 62 160 9.25e-36 135
MsaG028376 AT3G10480.2 61.682 107 40 1 2 108 84 189 1.16e-35 139
MsaG028376 AT4G17980.2 61.616 99 34 2 1 95 62 160 1.52e-35 135
MsaG028376 AT1G34180.3 57.009 107 45 1 2 108 73 178 2.85e-35 138
MsaG028376 AT1G34180.4 57.009 107 45 1 2 108 85 190 3.20e-35 138
MsaG028376 AT1G34190.1 57.944 107 44 1 2 108 73 178 4.30e-35 139
MsaG028376 AT3G10480.1 62.037 108 39 2 2 108 84 190 4.33e-35 138
MsaG028376 AT4G35580.1 51.613 124 60 0 2 125 66 189 5.55e-35 139
MsaG028376 AT4G35580.2 51.613 124 60 0 2 125 66 189 5.64e-35 139
MsaG028376 AT5G46590.1 59.000 100 39 2 1 98 62 161 6.08e-35 134
MsaG028376 AT4G35580.3 51.613 124 60 0 2 125 66 189 6.31e-35 139
MsaG028376 AT3G10480.3 62.037 108 39 2 2 108 117 223 1.05e-34 138
MsaG028376 AT1G34180.1 57.009 107 45 1 2 108 73 178 1.32e-34 138
MsaG028376 AT1G34180.2 57.009 107 45 1 2 108 85 190 1.39e-34 138
MsaG028376 AT1G33060.2 60.417 96 38 0 2 97 81 176 1.88e-34 139
MsaG028376 AT3G15500.1 59.375 96 39 0 2 97 69 164 1.89e-34 133
MsaG028376 AT1G33060.1 60.417 96 38 0 2 97 81 176 1.89e-34 139
MsaG028376 AT4G27410.2 59.375 96 39 0 2 97 69 164 2.52e-34 132
MsaG028376 AT4G27410.3 59.375 96 39 0 2 97 86 181 4.59e-34 132
MsaG028376 AT1G33280.1 52.778 108 46 2 2 109 66 168 5.92e-34 131
MsaG028376 AT3G10490.1 59.259 108 42 2 2 108 84 190 1.26e-33 129
MsaG028376 AT1G52890.1 49.580 119 57 1 2 120 69 184 1.93e-33 130
MsaG028376 AT3G18400.1 57.732 97 40 1 2 98 60 155 2.33e-33 130
MsaG028376 AT5G24590.2 56.566 99 42 1 2 99 70 168 2.80e-33 133
MsaG028376 AT4G10350.2 52.041 98 47 0 2 99 20 117 6.17e-33 128
MsaG028376 AT3G10490.2 59.259 108 42 2 2 108 84 190 1.15e-32 131
MsaG028376 AT5G39610.1 59.375 96 37 2 2 95 75 170 1.44e-32 127
MsaG028376 AT4G10350.1 52.041 98 47 0 2 99 67 164 1.72e-32 128
MsaG028376 AT2G24430.2 60.825 97 35 2 2 96 71 166 2.74e-32 127
MsaG028376 AT2G24430.1 60.825 97 35 2 2 96 71 166 2.74e-32 127
MsaG028376 AT5G61430.2 58.763 97 38 2 2 96 8 104 2.78e-32 125
MsaG028376 AT3G12977.2 55.670 97 42 1 2 97 20 116 4.30e-32 124
MsaG028376 AT1G32510.1 44.375 160 69 6 1 144 62 217 4.85e-32 125
MsaG028376 AT1G69490.1 40.223 179 93 6 2 174 64 234 6.35e-32 124
MsaG028376 AT1G79580.3 55.789 95 38 2 2 95 75 166 8.02e-32 127
MsaG028376 AT1G79580.5 55.789 95 38 2 2 95 75 166 8.02e-32 127
MsaG028376 AT1G79580.4 55.789 95 38 2 2 95 75 166 8.02e-32 127
MsaG028376 AT1G79580.2 55.789 95 38 2 2 95 75 166 8.02e-32 127
MsaG028376 AT1G79580.1 55.789 95 38 2 2 95 75 166 8.02e-32 127
MsaG028376 AT5G61430.1 58.763 97 38 2 2 96 71 167 9.66e-32 126
MsaG028376 AT5G07680.2 58.763 97 38 2 2 96 58 154 1.11e-31 125
MsaG028376 AT5G07680.1 58.163 98 39 2 2 97 72 169 1.17e-31 125
MsaG028376 AT1G71930.2 54.255 94 41 1 2 95 67 158 1.27e-31 125
MsaG028376 AT1G71930.1 54.255 94 41 1 2 95 67 158 1.27e-31 125
MsaG028376 AT3G12977.1 55.670 97 42 1 2 97 73 169 2.72e-31 123
MsaG028376 AT4G36160.2 53.191 94 42 1 2 95 72 163 3.10e-31 125
MsaG028376 AT4G36160.1 53.191 94 42 1 2 95 68 159 3.16e-31 125
MsaG028376 AT4G36160.3 53.191 94 42 1 2 95 68 159 3.16e-31 125
MsaG028376 AT5G62380.2 54.255 94 41 1 2 95 65 156 3.44e-31 125
MsaG028376 AT5G62380.1 54.255 94 41 1 2 95 65 156 3.44e-31 125
MsaG028376 AT1G77450.1 56.436 101 40 3 2 99 65 164 3.47e-31 122
MsaG028376 AT3G15170.1 56.863 102 40 2 2 99 75 176 3.73e-31 124
MsaG028376 AT1G12260.2 50.485 103 49 2 2 104 65 165 3.88e-31 124
MsaG028376 AT1G62700.1 50.000 106 51 2 2 107 65 168 3.96e-31 125
MsaG028376 AT1G62700.2 50.000 106 51 2 2 107 65 168 3.96e-31 125
MsaG028376 AT3G29035.1 39.181 171 72 3 2 142 79 247 5.00e-31 123
MsaG028376 AT1G12260.1 50.485 103 49 2 2 104 65 165 5.43e-31 125
MsaG028376 AT2G18060.3 52.128 94 43 1 2 95 67 158 8.32e-31 124
MsaG028376 AT2G18060.1 52.128 94 43 1 2 95 67 158 8.32e-31 124
MsaG028376 AT3G49530.2 53.000 100 46 1 2 100 70 169 9.01e-31 125
MsaG028376 AT5G46590.2 59.000 100 35 3 1 99 62 156 1.18e-30 121
MsaG028376 AT3G49530.1 53.000 100 46 1 2 100 70 169 1.78e-30 125
MsaG028376 AT2G46770.1 41.727 139 65 2 2 124 74 212 2.08e-30 123
MsaG028376 AT5G66300.1 50.495 101 49 1 2 102 70 169 4.83e-30 120
MsaG028376 AT5G18270.2 58.763 97 37 2 2 95 76 172 5.06e-30 121
MsaG028376 AT5G18270.1 58.763 97 37 2 2 95 76 172 5.52e-30 121
MsaG028376 AT1G56010.1 54.545 99 42 1 2 97 8 106 1.11e-29 118
MsaG028376 AT1G32870.2 56.881 109 42 3 2 108 67 172 1.20e-29 123
MsaG028376 AT5G53950.1 57.426 101 39 2 2 98 72 172 1.36e-29 120
MsaG028376 AT1G32870.1 56.881 109 42 3 2 108 67 172 1.43e-29 123
MsaG028376 AT1G32870.3 56.881 109 42 3 2 108 103 208 1.60e-29 123
MsaG028376 AT1G76420.1 57.732 97 38 2 2 96 77 172 2.24e-29 119
MsaG028376 AT1G01720.1 55.556 99 37 3 2 95 62 158 2.41e-29 118
MsaG028376 AT3G61910.1 45.045 111 46 2 2 98 69 178 3.56e-29 119
MsaG028376 AT1G56010.2 54.545 99 42 1 2 97 75 173 3.82e-29 118
MsaG028376 AT5G08790.1 42.759 145 76 3 2 140 62 205 4.23e-29 117
MsaG028376 AT3G04070.2 52.336 107 39 3 2 96 65 171 4.45e-29 118
MsaG028376 AT3G04060.1 57.732 97 38 2 2 95 75 171 6.39e-29 118
MsaG028376 AT1G32770.1 48.077 104 44 2 2 95 74 177 6.45e-29 118
MsaG028376 AT2G02450.1 57.576 99 37 3 2 98 106 201 6.79e-29 119
MsaG028376 AT2G02450.2 56.122 98 40 2 2 98 106 201 6.85e-29 119
MsaG028376 AT2G33480.3 53.191 94 44 0 2 95 10 103 8.82e-29 114
MsaG028376 AT5G64060.1 46.296 108 57 1 1 108 62 168 8.97e-29 118
MsaG028376 AT4G28530.1 44.586 157 69 6 2 156 81 221 1.22e-28 117
MsaG028376 AT5G09330.5 50.000 108 53 1 1 108 62 168 1.50e-28 119
MsaG028376 AT5G09330.2 50.000 108 53 1 1 108 62 168 1.50e-28 119
MsaG028376 AT5G09330.4 50.000 108 53 1 1 108 62 168 1.50e-28 119
MsaG028376 AT5G09330.3 50.000 108 53 1 1 108 62 168 1.50e-28 119
MsaG028376 AT5G09330.1 50.000 108 53 1 1 108 62 168 1.50e-28 119
MsaG028376 AT2G17040.1 49.153 118 50 4 2 116 61 171 1.82e-28 115
MsaG028376 AT1G61110.1 50.442 113 43 3 2 101 71 183 2.36e-28 116
MsaG028376 AT3G15510.1 51.818 110 40 3 2 98 72 181 4.39e-28 116
MsaG028376 AT2G33480.1 53.191 94 44 0 2 95 67 160 9.83e-28 113
MsaG028376 AT5G22290.1 54.945 91 35 1 2 92 77 161 1.57e-27 114
MsaG028376 AT5G63790.2 53.535 99 40 3 2 95 115 212 2.18e-27 113
MsaG028376 AT5G63790.1 53.535 99 40 3 2 95 105 202 2.21e-27 113
MsaG028376 AT2G33480.2 53.191 94 42 1 2 95 67 158 2.70e-27 111
MsaG028376 AT5G13180.1 55.208 96 40 2 2 95 66 160 5.42e-27 110
MsaG028376 AT3G04070.1 45.528 123 39 3 2 96 65 187 6.78e-27 112
MsaG028376 AT3G44290.2 53.846 91 35 2 2 92 70 153 9.16e-26 106
MsaG028376 AT3G44290.1 53.846 91 35 2 2 92 70 153 2.52e-25 107
MsaG028376 AT5G39820.1 42.623 122 45 3 2 99 75 195 3.05e-25 107
MsaG028376 AT1G26870.1 44.545 110 50 2 2 100 71 180 3.44e-25 108
MsaG028376 AT3G10490.4 58.889 90 35 2 20 108 2 90 8.29e-25 106
MsaG028376 AT3G10490.3 58.889 90 35 2 20 108 2 90 8.29e-25 106
MsaG028376 AT2G27300.1 50.526 95 40 1 2 96 70 157 8.30e-25 106
MsaG028376 AT2G43000.1 50.515 97 47 1 2 97 73 169 2.59e-24 103
MsaG028376 AT1G52880.1 53.271 107 38 4 2 96 72 178 1.56e-23 102
MsaG028376 AT4G01520.1 45.263 95 44 3 1 92 62 151 5.58e-21 94.4
MsaG028376 AT2G43000.2 48.889 90 45 1 9 97 2 91 7.41e-21 91.3
MsaG028376 AT3G44350.2 44.330 97 52 2 2 96 65 161 3.41e-20 90.5
MsaG028376 AT4G01540.2 45.263 95 44 3 1 92 62 151 3.42e-20 94.0
MsaG028376 AT4G01540.4 44.444 99 47 3 1 96 62 155 3.73e-20 93.2
MsaG028376 AT4G01540.3 44.211 95 45 2 1 92 62 151 3.85e-20 93.6
MsaG028376 AT4G01540.1 45.263 95 44 3 1 92 62 151 5.07e-20 94.0
MsaG028376 AT5G22380.1 45.745 94 48 3 2 92 66 159 5.85e-19 87.0
MsaG028376 AT4G01550.1 46.875 96 43 3 3 95 63 153 1.71e-18 89.0
MsaG028376 AT5G04395.1 47.436 78 38 1 2 76 96 173 2.30e-18 84.0
MsaG028376 AT2G18060.2 45.070 71 37 1 25 95 2 70 2.59e-18 85.9
MsaG028376 AT3G12910.1 42.727 110 48 3 2 96 77 186 3.94e-18 85.9
MsaG028376 AT5G22290.2 53.731 67 25 1 26 92 2 62 2.54e-17 82.0
MsaG028376 AT3G44350.1 43.182 88 48 2 2 87 65 152 6.16e-17 79.0
MsaG028376 AT3G04420.2 38.542 96 54 1 3 98 63 153 4.86e-16 79.3
MsaG028376 AT3G04420.1 38.542 96 54 1 3 98 63 153 1.61e-15 78.6
MsaG028376 AT4G28530.2 83.333 36 6 0 2 37 81 116 5.24e-12 67.8
MsaG028376 AT1G02220.1 39.706 68 38 1 3 70 63 127 2.90e-11 66.2
MsaG028376 AT1G02230.1 37.634 93 48 3 3 92 63 148 9.86e-11 64.3

Find 139 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCGTCATTTACTAATTA+TGG 0.136269 5:+61475834 MsaT028376.1:CDS
TTTGATGCATCGTGTTTATT+TGG 0.178984 5:+61478065 MsaT028376.1:CDS
CCCATGGCTCTCGTACAAAT+TGG 0.222002 5:+61475040 MsaT028376.1:CDS
CGACAAGGGATCATGGAAAT+TGG 0.232604 5:+61475769 MsaT028376.1:CDS
CCATATTGTCCTCCAACTTT+TGG 0.268556 5:-61475579 None:intergenic
ACGAATCGTGCAACCAAATC+TGG 0.280386 5:+61474922 MsaT028376.1:CDS
ATCAAAGAAAATAGCAAGTT+TGG 0.283752 5:+61478020 MsaT028376.1:CDS
TTGATTGGTCACTTGTTATC+TGG 0.283981 5:-61475623 None:intergenic
TCTATAGAGTGTTTATTGTT+TGG 0.309883 5:-61477901 None:intergenic
TTCACTTAATCATGCTTCTC+AGG 0.318478 5:+61476723 MsaT028376.1:CDS
CCAAAAGTTGGAGGACAATA+TGG 0.318542 5:+61475579 MsaT028376.1:CDS
ATCCAAGGCTTGTAAATAAC+TGG 0.328844 5:-61476761 None:intergenic
TGTTTATTGTTTGGCATCAT+TGG 0.334489 5:-61477892 None:intergenic
TGAGATCATTGAGGAGATAA+AGG 0.346824 5:+61477777 MsaT028376.1:CDS
TCTTCTATAAAATTCTCATT+GGG 0.351374 5:-61477718 None:intergenic
TGCCTTCCAATACCCAGATT+TGG 0.362335 5:-61474935 None:intergenic
TGAAACCACTCCTAGCTTGC+AGG 0.368248 5:+61475098 MsaT028376.1:CDS
ATCTTCTATAAAATTCTCAT+TGG 0.369560 5:-61477719 None:intergenic
GGCTTGCTCACCTCACAATA+TGG 0.386993 5:+61475716 MsaT028376.1:CDS
GTCCAGTTATTTACAAGCCT+TGG 0.389203 5:+61476759 MsaT028376.1:CDS
GGACAATATGGTAATATTAC+AGG 0.392348 5:+61475591 MsaT028376.1:CDS
TCAAAAGAGGTTTGGTTTGT+TGG 0.393311 5:+61478177 MsaT028376.1:CDS
GTAGTTGGAAGACTAAATAA+TGG 0.394217 5:-61475813 None:intergenic
ATAGCTCAACGTTTGTTGAT+TGG 0.409354 5:-61475638 None:intergenic
GGAATGAGTCAAAAGAGGTT+TGG 0.411979 5:+61478169 MsaT028376.1:CDS
ATCTTTCTTCATGAAGTTCA+AGG 0.415168 5:+61477963 MsaT028376.1:CDS
TCTGGGTATTGGAAGGCAAC+CGG 0.415206 5:+61474940 MsaT028376.1:CDS
AGGCCTATGTGATGGGGAAA+TGG 0.422475 5:+61477983 MsaT028376.1:CDS
GTAGAGAATTTGAGATGGAT+TGG 0.422814 5:+61476961 MsaT028376.1:CDS
AATTGCAAATGATATCTTGC+AGG 0.423405 5:+61476693 MsaT028376.1:CDS
TGGGATATGTTGACAAAACT+TGG 0.424746 5:+61476869 MsaT028376.1:CDS
CATTGTGTTCCCTTGATGTT+TGG 0.427811 5:-61477206 None:intergenic
ATTTCTTTAGCCCTCGTGAT+AGG 0.430194 5:+61474878 MsaT028376.1:CDS
AAATTATTTGATGCAAATGA+AGG 0.433232 5:+61475695 MsaT028376.1:CDS
CACTTGTTATCTGGTTTGCA+TGG 0.440837 5:-61475614 None:intergenic
CCATGGCTCTCGTACAAATT+GGG 0.441564 5:+61475041 MsaT028376.1:CDS
AGATGCAAAGATGATGATAA+TGG 0.441835 5:+61477930 MsaT028376.1:CDS
CTCTTCCTCGATAGTAAACT+AGG 0.442628 5:-61475014 None:intergenic
GTGCAACCAAATCTGGGTAT+TGG 0.448103 5:+61474929 MsaT028376.1:CDS
CTTGGATGGAGGGTATGTTA+TGG 0.457226 5:-61475859 None:intergenic
GTTTACTATGCCTCCATTGG+AGG 0.458982 5:+61476618 MsaT028376.1:CDS
TACTATGCCTCCATTGGAGG+TGG 0.460939 5:+61476621 MsaT028376.1:CDS
GACAAGGGATCATGGAAATT+GGG 0.465207 5:+61475770 MsaT028376.1:CDS
GATTCGTTCTAGATCCATTC+GGG 0.466518 5:-61474906 None:intergenic
TTAGTAAATGACGAAGTAGT+TGG 0.468200 5:-61475828 None:intergenic
GTTGGTGTAAAATGTGAGAA+AGG 0.471420 5:+61478195 MsaT028376.1:CDS
AGAAGGAGAACCTGCAAGCT+AGG 0.472184 5:-61475108 None:intergenic
AAAGGTTAGTCATAGAATGT+TGG 0.473148 5:+61477795 MsaT028376.1:CDS
AAAGTAAAATCATCTTCATA+AGG 0.480913 5:-61476802 None:intergenic
AGGATTAAGTGTGGTCTTGA+AGG 0.480916 5:+61478215 MsaT028376.1:CDS
TAGGTTTACTATGCCTCCAT+TGG 0.482015 5:+61476615 MsaT028376.1:CDS
TCCTCAATGATCTCAAAGCT+TGG 0.489482 5:-61477769 None:intergenic
GTGGAGGAATTTCCTCAAGT+TGG 0.490438 5:+61476640 MsaT028376.1:CDS
CGATTCGTTCTAGATCCATT+CGG 0.490510 5:-61474907 None:intergenic
AACACAAGATCTATAGAGAT+AGG 0.493319 5:+61476901 MsaT028376.1:CDS
ATGATGTCAATGAATTCTCA+TGG 0.496801 5:+61477674 MsaT028376.1:CDS
TAGTTGGAAGACTAAATAAT+GGG 0.497545 5:-61475812 None:intergenic
CCCAATTTGTACGAGAGCCA+TGG 0.500594 5:-61475041 None:intergenic
TCACTTAATCATGCTTCTCA+GGG 0.500637 5:+61476724 MsaT028376.1:CDS
AAGTTCAAGGCCTATGTGAT+GGG 0.501395 5:+61477976 MsaT028376.1:CDS
GATGCATCGTGTTTATTTGG+AGG 0.502081 5:+61478068 MsaT028376.1:CDS
AATGGTACGACAAGGGATCA+TGG 0.502815 5:+61475762 MsaT028376.1:CDS
TTGAGCTATACTCTGAAAGA+AGG 0.503430 5:+61475652 MsaT028376.1:CDS
ATGAATATCGTCTTGATGAG+AGG 0.503672 5:+61475070 MsaT028376.1:CDS
AGCATGAAATATCCTCCATT+GGG 0.507467 5:-61477182 None:intergenic
ACAGTGATTGTGCCAAAAGT+TGG 0.507511 5:+61475567 MsaT028376.1:CDS
CCAATTTGTACGAGAGCCAT+GGG 0.509568 5:-61475040 None:intergenic
CATGAATGATATGAGCTCCA+TGG 0.513072 5:+61476849 MsaT028376.1:CDS
AATCACATGAGAGAGTCAAA+TGG 0.514617 5:+61478148 MsaT028376.1:CDS
TAGCATGAAATATCCTCCAT+TGG 0.516471 5:-61477183 None:intergenic
AATTCTCAAGCTCGAGCAGT+AGG 0.521751 5:+61474979 MsaT028376.1:CDS
GGAAATTCCTCCACCTCCAA+TGG 0.528982 5:-61476631 None:intergenic
TTTCATGCTACCAAACATCA+AGG 0.530340 5:+61477196 MsaT028376.1:CDS
GAGACTTCTCTCAATATCAA+TGG 0.542689 5:+61475744 MsaT028376.1:CDS
AAGATGATGATAATGGAGAA+TGG 0.544768 5:+61477937 MsaT028376.1:CDS
ATGAATGATATGAGCTCCAT+GGG 0.545336 5:+61476850 MsaT028376.1:CDS
TATGCCTCCATTGGAGGTGG+AGG 0.545899 5:+61476624 MsaT028376.1:CDS
TGAGCTATACTCTGAAAGAA+GGG 0.546148 5:+61475653 MsaT028376.1:CDS
CGAATCGTGCAACCAAATCT+GGG 0.546533 5:+61474923 MsaT028376.1:CDS
GAAGTTCAAGGCCTATGTGA+TGG 0.547907 5:+61477975 MsaT028376.1:CDS
TCATGGGGATTCATCAATGA+TGG 0.548361 5:+61477691 MsaT028376.1:CDS
CTGGGTATTGGAAGGCAACC+GGG 0.551323 5:+61474941 MsaT028376.1:CDS
TGATGTCAATGAATTCTCAT+GGG 0.556387 5:+61477675 MsaT028376.1:CDS
CAAATTCTCTACCATCCGCT+TGG 0.560463 5:-61476950 None:intergenic
TCCAAGCTTTGAGATCATTG+AGG 0.563456 5:+61477768 MsaT028376.1:CDS
CTGTACTCTTCTTGATCACA+CGG 0.568059 5:-61475548 None:intergenic
CAAATGGAATGAGTCAAAAG+AGG 0.568226 5:+61478164 MsaT028376.1:CDS
TACTTTCGTCAGAATATGAG+CGG 0.570391 5:+61477623 MsaT028376.1:intron
ATTCTCAAGCTCGAGCAGTA+GGG 0.570616 5:+61474980 MsaT028376.1:CDS
AGCACAAAATGTTGTCCCAA+TGG 0.571545 5:+61477167 MsaT028376.1:CDS
GTTCCATTTCCCCATCACAT+AGG 0.572384 5:-61477986 None:intergenic
AGTGTGCAAGGATGCAACAT+AGG 0.574842 5:+61476596 MsaT028376.1:CDS
TGAATTTGAAGTGTTTGTGA+AGG 0.576360 5:-61477862 None:intergenic
TTCTCAAGCTCGAGCAGTAG+GGG 0.579595 5:+61474981 MsaT028376.1:CDS
TGAATATCGTCTTGATGAGA+GGG 0.582090 5:+61475071 MsaT028376.1:CDS
GTGATTGTGCCAAAAGTTGG+AGG 0.584888 5:+61475570 MsaT028376.1:CDS
CTTCCTTGTTGTGTCACATG+AGG 0.585636 5:-61476667 None:intergenic
AGGGAACACAATGGCATGCA+AGG 0.586165 5:+61477216 MsaT028376.1:CDS
TATAGAGATAGGTGATCTAG+AGG 0.589067 5:+61476912 MsaT028376.1:CDS
ATTGCAAATGATATCTTGCA+GGG 0.597558 5:+61476694 MsaT028376.1:CDS
TTCGGGTACTTCCTATCACG+AGG 0.598759 5:-61474889 None:intergenic
AACCAAATCTGGGTATTGGA+AGG 0.600307 5:+61474933 MsaT028376.1:CDS
TCTAGAGGATCAAGAGTCAA+AGG 0.606182 5:+61476927 MsaT028376.1:CDS
TCTTCCTCGATAGTAAACTA+GGG 0.609563 5:-61475013 None:intergenic
GGGATATGTTGACAAAACTT+GGG 0.610464 5:+61476870 MsaT028376.1:CDS
AGAGTCAAAGGCCAAGCGGA+TGG 0.610665 5:+61476939 MsaT028376.1:CDS
AACTTGGTGAAATAATGTCA+TGG 0.614916 5:-61477836 None:intergenic
CATAACATACCCTCCATCCA+AGG 0.617588 5:+61475860 MsaT028376.1:CDS
ACAAAATGTTGTCCCAATGG+AGG 0.618611 5:+61477170 MsaT028376.1:CDS
GTATTGGAAGGCAACCGGGA+AGG 0.620851 5:+61474945 MsaT028376.1:CDS
CTTCTATAAAATTCTCATTG+GGG 0.621652 5:-61477717 None:intergenic
ACATGAGGTATTCCAACTTG+AGG 0.622240 5:-61476652 None:intergenic
GGATGGTAGAGAATTTGAGA+TGG 0.631431 5:+61476956 MsaT028376.1:CDS
CGTGATAGGAAGTACCCGAA+TGG 0.632326 5:+61474892 MsaT028376.1:CDS
AATTCCTCCACCTCCAATGG+AGG 0.633153 5:-61476628 None:intergenic
AAAACCCTAGTTTACTATCG+AGG 0.634321 5:+61475009 MsaT028376.1:CDS
ATGTGAGAAAGGATTAAGTG+TGG 0.635962 5:+61478206 MsaT028376.1:CDS
GTCTCATGTACCATATTGTG+AGG 0.636414 5:-61475726 None:intergenic
TGTACCACATGGGCTAGCCA+TGG 0.637402 5:+61478273 MsaT028376.1:CDS
TCAATATCAATGGTACGACA+AGG 0.648156 5:+61475754 MsaT028376.1:CDS
TTCATGCTACCAAACATCAA+GGG 0.649844 5:+61477197 MsaT028376.1:CDS
ATACCTCATGTGACACAACA+AGG 0.650067 5:+61476664 MsaT028376.1:CDS
AGAAGATCAAGGGTTAGCCA+TGG 0.651875 5:-61478290 None:intergenic
GAGCTATACTCTGAAAGAAG+GGG 0.652301 5:+61475654 MsaT028376.1:CDS
TACCTGGGAATGATATGGAG+TGG 0.652400 5:+61474854 None:intergenic
GAGAACCTGCAAGCTAGGAG+TGG 0.654041 5:-61475103 None:intergenic
CAATATCAATGGTACGACAA+GGG 0.658341 5:+61475755 MsaT028376.1:CDS
TTTGGCGCTTTGTACCACAT+GGG 0.659507 5:+61478263 MsaT028376.1:CDS
GATGTCAATGAATTCTCATG+GGG 0.662201 5:+61477676 MsaT028376.1:CDS
ATATTGCACTAGAGTGTGCA+AGG 0.673783 5:+61476584 MsaT028376.1:CDS
ATGATGATAATGGAGAATGG+AGG 0.674089 5:+61477940 MsaT028376.1:CDS
ATCAAGAGTCAAAGGCCAAG+CGG 0.676267 5:+61476935 MsaT028376.1:CDS
TATCGAGGAAGAGCACCCCA+TGG 0.678830 5:+61475024 MsaT028376.1:CDS
TTAGCCATGGCTAGCCCATG+TGG 0.679756 5:-61478277 None:intergenic
GTGTTTGTGAAGGAACAACT+TGG 0.687378 5:-61477852 None:intergenic
AGTTCAAGGCCTATGTGATG+GGG 0.690612 5:+61477977 MsaT028376.1:CDS
CAAACATCAAGGGAACACAA+TGG 0.716596 5:+61477207 MsaT028376.1:CDS
TCGGGTACTTCCTATCACGA+GGG 0.722170 5:-61474888 None:intergenic
CAATTTGTACGAGAGCCATG+GGG 0.773296 5:-61475039 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 61474868 61478299 61474868 ID=MsaG028376
Chr5 mRNA 61474868 61478299 61474868 ID=MsaT028376.1;Parent=MsaG028376
Chr5 exon 61474868 61475119 61474868 ID=MsaT028376.1.exon1;Parent=MsaT028376.1
Chr5 CDS 61474868 61475119 61474868 ID=cds.MsaT028376.1;Parent=MsaT028376.1
Chr5 exon 61475531 61475881 61475531 ID=MsaT028376.1.exon2;Parent=MsaT028376.1
Chr5 CDS 61475531 61475881 61475531 ID=cds.MsaT028376.1;Parent=MsaT028376.1
Chr5 exon 61476580 61477008 61476580 ID=MsaT028376.1.exon3;Parent=MsaT028376.1
Chr5 CDS 61476580 61477008 61476580 ID=cds.MsaT028376.1;Parent=MsaT028376.1
Chr5 exon 61477162 61477237 61477162 ID=MsaT028376.1.exon4;Parent=MsaT028376.1
Chr5 CDS 61477162 61477237 61477162 ID=cds.MsaT028376.1;Parent=MsaT028376.1
Chr5 exon 61477635 61478299 61477635 ID=MsaT028376.1.exon5;Parent=MsaT028376.1
Chr5 CDS 61477635 61478299 61477635 ID=cds.MsaT028376.1;Parent=MsaT028376.1
Gene Sequence

>MsaT028376.1

ATGGAGTGGTATTTCTTTAGCCCTCGTGATAGGAAGTACCCGAATGGATCTAGAACGAATCGTGCAACCAAATCTGGGTATTGGAAGGCAACCGGGAAGGATCGAAAAGTGAATTCTCAAGCTCGAGCAGTAGGGGTGAAGAAAACCCTAGTTTACTATCGAGGAAGAGCACCCCATGGCTCTCGTACAAATTGGGTTATGCATGAATATCGTCTTGATGAGAGGGAATGTGAAACCACTCCTAGCTTGCAGGATGCATATGCTCTTTGCCGTGTGATCAAGAAGAGTACAGTGATTGTGCCAAAAGTTGGAGGACAATATGGTAATATTACAGGCCATGCAAACCAGATAACAAGTGACCAATCAACAAACGTTGAGCTATACTCTGAAAGAAGGGGTGAAAATTTAGCTAGTTCAAATTATTTGATGCAAATGAAGGCTTGCTCACCTCACAATATGGTACATGAGACTTCTCTCAATATCAATGGTACGACAAGGGATCATGGAAATTGGGCGCAATTCTCATCTGAAGACCCATTATTTAGTCTTCCAACTACTTCGTCATTTACTAATTATGGAGCCATAACATACCCTCCATCCAAGGTGGATATTGCACTAGAGTGTGCAAGGATGCAACATAGGTTTACTATGCCTCCATTGGAGGTGGAGGAATTTCCTCAAGTTGGAATACCTCATGTGACACAACAAGGAAGCAGAATTGCAAATGATATCTTGCAGGGAATTCTTTCACTTAATCATGCTTCTCAGGGATTGATGAATCAGTCCAGTTATTTACAAGCCTTGGATGACAATGAAAATTATGCTCCTTATGAAGATGATTTTACTTTCATGGTTGACACTAACTACAATCACATGAATGATATGAGCTCCATGGGATATGTTGACAAAACTTGGGAAGATTTGAACACAAGATCTATAGAGATAGGTGATCTAGAGGATCAAGAGTCAAAGGCCAAGCGGATGGTAGAGAATTTGAGATGGATTGGAATGCCAAGAGAAGATTTTGAAAAGATGGAAGCACAAAATGTTGTCCCAATGGAGGATATTTCATGCTACCAAACATCAAGGGAACACAATGGCATGCAAGAATATGAGCGGCACAATAAGAACCTTGAACAAAATGACAATGATGTCAATGAATTCTCATGGGGATTCATCAATGATGGTGACCCCAATGAGAATTTTATAGAAGATGGAAACATGGATGATTACTCAAATTCTCCAAGCTTTGAGATCATTGAGGAGATAAAGGTTAGTCATAGAATGTTGGTTTCAACTCAACAAGTAGCCATGACATTATTTCACCAAGTTGTTCCTTCACAAACACTTCAAATTCAACTCAATCCAATGATGCCAAACAATAAACACTCTATAGAGAACGTAGATGCAAAGATGATGATAATGGAGAATGGAGGATCTTTCTTCATGAAGTTCAAGGCCTATGTGATGGGGAAATGGAACCTATTCAACCCATCAAAGAAAATAGCAAGTTTGGTTGTATTTGTATTTGCACTTCTTTTGATGCATCGTGTTTATTTGGAGGAAGAAGTAGAAATCTGCAAACCAAAACCAAAAAAGATGAAAGAGAAAAGAATGAGAGAATCACATGAGAGAGTCAAATGGAATGAGTCAAAAGAGGTTTGGTTTGTTGGTGTAAAATGTGAGAAAGGATTAAGTGTGGTCTTGAAGGAAATAAGCATTTTCTTCACCATATTTTTGGCGCTTTGTACCACATGGGCTAGCCATGGCTAA
Protein sequence

>MsaT028376.1

MEWYFFSPRDRKYPNGSRTNRATKSGYWKATGKDRKVNSQARAVGVKKTLVYYRGRAPHGSRTNWVMHEYRLDERECETTPSLQDAYALCRVIKKSTVIVPKVGGQYGNITGHANQITSDQSTNVELYSERRGENLASSNYLMQMKACSPHNMVHETSLNINGTTRDHGNWAQFSSEDPLFSLPTTSSFTNYGAITYPPSKVDIALECARMQHRFTMPPLEVEEFPQVGIPHVTQQGSRIANDILQGILSLNHASQGLMNQSSYLQALDDNENYAPYEDDFTFMVDTNYNHMNDMSSMGYVDKTWEDLNTRSIEIGDLEDQESKAKRMVENLRWIGMPREDFEKMEAQNVVPMEDISCYQTSREHNGMQEYERHNKNLEQNDNDVNEFSWGFINDGDPNENFIEDGNMDDYSNSPSFEIIEEIKVSHRMLVSTQQVAMTLFHQVVPSQTLQIQLNPMMPNNKHSIENVDAKMMIMENGGSFFMKFKAYVMGKWNLFNPSKKIASLVVFVFALLLMHRVYLEEEVEICKPKPKKMKEKRMRESHERVKWNESKEVWFVGVKCEKGLSVVLKEISIFFTIFLALCTTWASHG*