Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028490 | KEH26852.1 | 51.765 | 85 | 40 | 1 | 69 | 153 | 61 | 144 | 6.73e-18 | 89.7 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028490 | tr|A0A072UB72|A0A072UB72_MEDTR | 51.765 | 85 | 40 | 1 | 69 | 153 | 61 | 144 | 3.21e-18 | 89.7 |
Gene ID | Type | Classification |
---|---|---|
MsaG028490 | TF | B3 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000791 | MsaG028490 | 0.806108 | 1.635003e-49 | 2.543834e-47 |
MsaG001030 | MsaG028490 | 0.800383 | 2.472367e-48 | 3.371888e-46 |
MsaG001192 | MsaG028490 | 0.809235 | 3.568457e-50 | 5.982196e-48 |
MsaG001827 | MsaG028490 | 0.805809 | 1.888846e-49 | 2.918060e-47 |
MsaG001861 | MsaG028490 | 0.808911 | 4.185028e-50 | 6.961567e-48 |
MsaG001910 | MsaG028490 | 0.822554 | 3.923594e-53 | 9.235643e-51 |
MsaG002024 | MsaG028490 | 0.805799 | 1.897617e-49 | 2.930936e-47 |
MsaG002093 | MsaG028490 | 0.803004 | 7.209908e-49 | 1.043691e-46 |
MsaG002121 | MsaG028490 | 0.824665 | 1.264504e-53 | 3.151910e-51 |
MsaG002359 | MsaG028490 | 0.807338 | 9.016090e-50 | 1.444141e-47 |
MsaG003332 | MsaG028490 | 0.836462 | 1.687439e-56 | 5.905958e-54 |
MsaG003339 | MsaG028490 | 0.800895 | 1.946226e-48 | 2.685074e-46 |
MsaG003915 | MsaG028490 | 0.810629 | 1.794431e-50 | 3.111715e-48 |
MsaG004308 | MsaG028490 | 0.810074 | 2.360530e-50 | 4.038433e-48 |
MsaG005035 | MsaG028490 | 0.800607 | 2.227152e-48 | 3.052881e-46 |
MsaG005246 | MsaG028490 | 0.802563 | 8.883864e-49 | 1.273083e-46 |
MsaG005626 | MsaG028490 | 0.807169 | 9.788629e-50 | 1.561583e-47 |
MsaG005643 | MsaG028490 | 0.803194 | 6.588324e-49 | 9.579261e-47 |
MsaG006254 | MsaG028490 | 0.805760 | 1.933527e-49 | 2.983697e-47 |
MsaG006256 | MsaG028490 | 0.801322 | 1.593515e-48 | 2.219823e-46 |
MsaG006679 | MsaG028490 | 0.801898 | 1.215587e-48 | 1.715690e-46 |
MsaG006841 | MsaG028490 | 0.801566 | 1.420911e-48 | 1.990327e-46 |
MsaG007399 | MsaG028490 | 0.818713 | 2.963600e-52 | 6.300987e-50 |
MsaG007580 | MsaG028490 | 0.800884 | 1.956964e-48 | 2.699202e-46 |
MsaG007700 | MsaG028490 | 0.800156 | 2.748669e-48 | 3.729439e-46 |
MsaG008033 | MsaG028490 | 0.847040 | 2.804281e-59 | 1.375056e-56 |
MsaG008299 | MsaG028490 | 0.800193 | 2.702079e-48 | 3.669211e-46 |
MsaG008815 | MsaG028490 | 0.801225 | 1.667794e-48 | 2.318108e-46 |
MsaG008988 | MsaG028490 | 0.827313 | 2.991033e-54 | 8.023154e-52 |
MsaG009156 | MsaG028490 | 0.807541 | 8.167775e-50 | 1.314612e-47 |
MsaG009916 | MsaG028490 | 0.812723 | 6.317382e-51 | 1.153418e-48 |
MsaG011240 | MsaG028490 | 0.823026 | 3.049867e-53 | 7.271206e-51 |
MsaG011336 | MsaG028490 | 0.809363 | 3.350760e-50 | 5.634680e-48 |
MsaG011341 | MsaG028490 | 0.801333 | 1.585495e-48 | 2.209223e-46 |
MsaG011460 | MsaG028490 | 0.808906 | 4.193496e-50 | 6.974936e-48 |
MsaG011996 | MsaG028490 | 0.802604 | 8.714731e-49 | 1.250005e-46 |
MsaG012130 | MsaG028490 | 0.802245 | 1.032151e-48 | 1.468298e-46 |
MsaG012302 | MsaG028490 | 0.802849 | 7.759232e-49 | 1.119166e-46 |
MsaG013182 | MsaG028490 | 0.833825 | 7.749883e-56 | 2.506409e-53 |
MsaG013327 | MsaG028490 | 0.814478 | 2.607599e-51 | 4.974860e-49 |
MsaG013443 | MsaG028490 | 0.808335 | 5.546391e-50 | 9.098847e-48 |
MsaG014649 | MsaG028490 | 0.875018 | 8.878002e-68 | 1.277713e-64 |
MsaG016584 | MsaG028490 | 0.822366 | 4.337265e-53 | 1.015802e-50 |
MsaG017682 | MsaG028490 | 0.801156 | 1.722317e-48 | 2.390229e-46 |
MsaG018355 | MsaG028490 | 0.819479 | 1.987997e-52 | 4.312640e-50 |
MsaG018502 | MsaG028490 | 0.803088 | 6.929991e-49 | 1.005119e-46 |
MsaG018876 | MsaG028490 | 0.805241 | 2.481760e-49 | 3.783623e-47 |
MsaG019225 | MsaG028490 | 0.801291 | 1.616881e-48 | 2.250760e-46 |
MsaG019430 | MsaG028490 | 0.824707 | 1.236242e-53 | 3.084939e-51 |
MsaG019533 | MsaG028490 | 0.803884 | 4.747839e-49 | 7.013997e-47 |
MsaG020091 | MsaG028490 | 0.812494 | 7.087620e-51 | 1.286729e-48 |
MsaG020126 | MsaG028490 | 0.805386 | 2.315047e-49 | 3.541294e-47 |
MsaG020641 | MsaG028490 | 0.805789 | 1.906512e-49 | 2.944010e-47 |
MsaG020775 | MsaG028490 | 0.803957 | 4.585947e-49 | 6.786457e-47 |
MsaG020782 | MsaG028490 | 0.802284 | 1.013596e-48 | 1.443211e-46 |
MsaG021085 | MsaG028490 | 0.834889 | 4.204967e-56 | 1.403832e-53 |
MsaG021273 | MsaG028490 | 0.821745 | 6.030505e-53 | 1.389048e-50 |
MsaG021781 | MsaG028490 | 0.823416 | 2.475370e-53 | 5.964151e-51 |
MsaG021836 | MsaG028490 | 0.834126 | 6.522005e-56 | 2.128186e-53 |
MsaG022454 | MsaG028490 | 0.841465 | 8.672262e-58 | 3.545514e-55 |
MsaG022706 | MsaG028490 | 0.802090 | 1.110713e-48 | 1.574476e-46 |
MsaG022954 | MsaG028490 | 0.819927 | 1.572272e-52 | 3.451307e-50 |
MsaG023230 | MsaG028490 | 0.805329 | 2.378450e-49 | 3.633526e-47 |
MsaG023664 | MsaG028490 | 0.820419 | 1.214430e-52 | 2.700842e-50 |
MsaG023681 | MsaG028490 | 0.809846 | 2.641725e-50 | 4.494683e-48 |
MsaG023849 | MsaG028490 | 0.800602 | 2.232207e-48 | 3.059458e-46 |
MsaG024454 | MsaG028490 | 0.807426 | 8.637197e-50 | 1.386372e-47 |
MsaG025276 | MsaG028490 | 0.829038 | 1.153681e-54 | 3.248208e-52 |
MsaG025330 | MsaG028490 | 0.821725 | 6.094885e-53 | 1.403155e-50 |
MsaG025615 | MsaG028490 | 0.802861 | 7.715303e-49 | 1.113144e-46 |
MsaG026252 | MsaG028490 | 0.839159 | 3.449137e-57 | 1.311415e-54 |
MsaG027172 | MsaG028490 | 0.804981 | 2.811650e-49 | 4.260692e-47 |
MsaG027573 | MsaG028490 | 0.823594 | 2.250268e-53 | 5.448191e-51 |
MsaG028318 | MsaG028490 | 0.803812 | 4.912609e-49 | 7.245465e-47 |
MsaG028370 | MsaG028490 | 0.807745 | 7.397851e-50 | 1.196525e-47 |
MsaG028328 | MsaG028490 | 0.823329 | 2.594644e-53 | 6.236862e-51 |
MsaG028490 | MsaG028821 | 0.800889 | 1.952028e-48 | 2.692707e-46 |
MsaG028490 | MsaG029083 | 0.803243 | 6.438087e-49 | 9.371211e-47 |
MsaG028490 | MsaG029366 | 0.814808 | 2.205838e-51 | 4.243528e-49 |
MsaG028490 | MsaG031693 | 0.839650 | 2.576331e-57 | 9.947141e-55 |
MsaG028490 | MsaG031694 | 0.812153 | 8.406053e-51 | 1.513128e-48 |
MsaG028490 | MsaG032196 | 0.816449 | 9.544775e-52 | 1.914211e-49 |
MsaG028490 | MsaG033246 | 0.804604 | 3.366834e-49 | 5.057540e-47 |
MsaG028490 | MsaG033967 | 0.813969 | 3.373632e-51 | 6.354806e-49 |
MsaG028490 | MsaG034683 | 0.802666 | 8.461342e-49 | 1.215379e-46 |
MsaG028490 | MsaG035152 | 0.821177 | 8.147073e-53 | 1.848558e-50 |
MsaG028490 | MsaG035155 | 0.808891 | 4.226213e-50 | 7.026760e-48 |
MsaG028490 | MsaG035666 | 0.802599 | 8.731465e-49 | 1.252283e-46 |
MsaG028490 | MsaG035816 | 0.820028 | 1.491324e-52 | 3.282479e-50 |
MsaG028490 | MsaG035867 | 0.838234 | 5.967746e-57 | 2.204536e-54 |
MsaG028490 | MsaG036980 | 0.808919 | 4.166994e-50 | 6.933047e-48 |
MsaG028490 | MsaG038467 | 0.815642 | 1.442409e-51 | 2.834070e-49 |
MsaG028490 | MsaG038498 | 0.811307 | 1.281333e-50 | 2.259200e-48 |
MsaG028490 | MsaG039692 | 0.803072 | 6.983070e-49 | 1.012437e-46 |
MsaG028490 | MsaG039773 | 0.801817 | 1.262786e-48 | 1.779027e-46 |
MsaG028490 | MsaG040310 | 0.803769 | 5.015462e-49 | 7.389731e-47 |
MsaG028490 | MsaG041010 | 0.810370 | 2.039565e-50 | 3.514634e-48 |
MsaG028490 | MsaG041066 | 0.828924 | 1.228937e-54 | 3.448885e-52 |
MsaG028490 | MsaG041370 | 0.822469 | 4.105266e-53 | 9.641106e-51 |
MsaG028490 | MsaG041347 | 0.812984 | 5.543380e-51 | 1.018707e-48 |
MsaG028490 | MsaG041599 | 0.845789 | 6.127671e-59 | 2.881840e-56 |
MsaG028490 | MsaG041766 | 0.806521 | 1.339441e-49 | 2.104459e-47 |
MsaG028490 | MsaG042532 | 0.804704 | 3.210158e-49 | 4.833384e-47 |
MsaG028490 | MsaG042799 | 0.809097 | 3.819140e-50 | 6.381593e-48 |
MsaG028490 | MsaG043285 | 0.800834 | 2.003146e-48 | 2.759819e-46 |
MsaG028490 | MsaG043634 | 0.800185 | 2.712294e-48 | 3.682428e-46 |
MsaG028490 | MsaG043787 | 0.811310 | 1.279614e-50 | 2.256307e-48 |
MsaG028490 | MsaG043935 | 0.816032 | 1.181767e-51 | 2.344977e-49 |
MsaG028490 | MsaG043974 | 0.812962 | 5.603917e-51 | 1.029274e-48 |
MsaG028490 | MsaG043975 | 0.808954 | 4.096554e-50 | 6.821582e-48 |
MsaG028490 | MsaG044189 | 0.809086 | 3.838895e-50 | 6.412874e-48 |
MsaG028490 | MsaG044305 | 0.803694 | 5.196056e-49 | 7.642577e-47 |
MsaG028490 | MsaG044307 | 0.813686 | 3.893041e-51 | 7.281043e-49 |
MsaG028490 | MsaG044312 | 0.808008 | 6.506445e-50 | 1.059052e-47 |
MsaG028490 | MsaG044886 | 0.815221 | 1.788088e-51 | 3.475933e-49 |
MsaG028490 | MsaG045314 | 0.810289 | 2.123223e-50 | 3.651485e-48 |
MsaG028490 | MsaG046441 | 0.825580 | 7.705910e-54 | 1.969623e-51 |
MsaG028490 | MsaG047165 | 0.807321 | 9.089598e-50 | 1.455315e-47 |
MsaG028490 | MsaG000632 | 0.822278 | 4.544419e-53 | 1.061773e-50 |
MsaG028490 | MsaG000658 | 0.830372 | 5.482592e-55 | 1.603698e-52 |
MsaG028490 | MsaG008905 | 0.818970 | 2.593050e-52 | 5.550541e-50 |
MsaG028490 | MsaG011535 | 0.805712 | 1.978717e-49 | 3.050007e-47 |
MsaG028490 | MsaG013067 | 0.802177 | 1.065739e-48 | 1.513743e-46 |
MsaG028490 | MsaG018951 | 0.808638 | 4.783870e-50 | 7.905395e-48 |
MsaG028490 | MsaG033743 | 0.803668 | 5.262049e-49 | 7.734969e-47 |
MsaG028490 | MsaG034563 | 0.813726 | 3.814602e-51 | 7.141594e-49 |
MsaG028490 | MsaG032972 | 0.809101 | 3.811895e-50 | 6.370088e-48 |
MsaG028490 | MsaG032466 | 0.803771 | 5.009253e-49 | 7.381026e-47 |
MsaG028490 | MsaG033379 | 0.800991 | 1.860544e-48 | 2.572476e-46 |
MsaG028490 | MsaG030783 | 0.803647 | 5.314649e-49 | 7.808357e-47 |
MsaG028490 | MsaG031525 | 0.819544 | 1.921347e-52 | 4.174964e-50 |
MsaG028490 | MsaG033287 | 0.800183 | 2.714330e-48 | 3.685052e-46 |
MsaG028490 | MsaG036708 | 0.808075 | 6.297090e-50 | 1.026629e-47 |
MsaG028490 | MsaG037988 | 0.810172 | 2.249939e-50 | 3.858302e-48 |
MsaG028490 | MsaG039944 | 0.809931 | 2.533093e-50 | 4.318583e-48 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG028490 | MtrunA17_Chr2g0281511 | 30.769 | 117 | 79 | 1 | 38 | 154 | 6 | 120 | 7.23e-16 | 69.7 |
MsaG028490 | MtrunA17_Chr2g0309121 | 36.145 | 83 | 52 | 1 | 72 | 154 | 38 | 119 | 7.61e-15 | 67.0 |
MsaG028490 | MtrunA17_Chr6g0459981 | 38.043 | 92 | 53 | 2 | 53 | 144 | 62 | 149 | 2.53e-14 | 66.6 |
MsaG028490 | MtrunA17_Chr5g0430431 | 35.354 | 99 | 55 | 1 | 52 | 150 | 165 | 254 | 1.65e-12 | 63.2 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 39 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GCAAACTTATCACTTCAAAT+AGG | 0.238418 | 5:-63098644 | MsaT028490.1:CDS |
AAACCGCAAAAGTATGATTA+TGG | 0.295816 | 5:-63099176 | MsaT028490.1:CDS |
TCACCTAAGTATTTCTCATT+TGG | 0.308885 | 5:+63098618 | None:intergenic |
CAAACTTATCACTTCAAATA+GGG | 0.313955 | 5:-63098643 | MsaT028490.1:CDS |
TTGTTTAGCACTTCCCCAAT+AGG | 0.356270 | 5:-63098743 | MsaT028490.1:intron |
CAGTTGATAGATATCCTATT+GGG | 0.375059 | 5:+63098729 | None:intergenic |
TCTACGGTCCTTCACGAACT+TGG | 0.378944 | 5:+63098587 | None:intergenic |
AGTATGATTATGGATGGATT+AGG | 0.381484 | 5:-63099166 | MsaT028490.1:CDS |
ACAGGAGAGCATTACTTTGC+AGG | 0.396717 | 5:-63098694 | MsaT028490.1:CDS |
ACTCATTACACAGTGCCATT+TGG | 0.408378 | 5:+63099140 | None:intergenic |
TAAGTTTGCACTCATATTCT+CGG | 0.417716 | 5:+63098658 | None:intergenic |
GCAGTTGATAGATATCCTAT+TGG | 0.431455 | 5:+63098728 | None:intergenic |
GTATGATTATGGATGGATTA+GGG | 0.435600 | 5:-63099165 | MsaT028490.1:CDS |
ACCTCCACAAAGAGACGATT+GGG | 0.451863 | 5:+63098522 | None:intergenic |
AGAAATACTTAGGTGAAGAT+TGG | 0.462766 | 5:-63098611 | MsaT028490.1:CDS |
TCCTCCCAATCGTCTCTTTG+TGG | 0.465471 | 5:-63098526 | MsaT028490.1:CDS |
GTAATGCTCTCCTGTGTGTC+GGG | 0.473972 | 5:+63098702 | None:intergenic |
AGGTTGTCGACGCCGTCGTT+AGG | 0.482126 | 5:-63098503 | MsaT028490.1:CDS |
CGCAAAAGTATGATTATGGA+TGG | 0.485297 | 5:-63099172 | MsaT028490.1:CDS |
ACATCTCTTGTTGTTAGCTG+TGG | 0.496090 | 5:+63098866 | None:intergenic |
TATCACCTTTCCTTAGTCTA+CGG | 0.505505 | 5:+63098571 | None:intergenic |
GGTCCAAATGAGAAATACTT+AGG | 0.515401 | 5:-63098621 | MsaT028490.1:CDS |
TCGTGAAGGACCGTAGACTA+AGG | 0.541590 | 5:-63098581 | MsaT028490.1:CDS |
AACCTCCACAAAGAGACGAT+TGG | 0.547571 | 5:+63098521 | None:intergenic |
CAACAAGAGATGTCAAGTAA+TGG | 0.551729 | 5:-63098856 | MsaT028490.1:intron |
CTACGGTCCTTCACGAACTT+GGG | 0.555110 | 5:+63098588 | None:intergenic |
GTTCAAGGGAAATACAATCA+AGG | 0.557132 | 5:-63098970 | MsaT028490.1:CDS |
TCCCAATCGTCTCTTTGTGG+AGG | 0.560244 | 5:-63098523 | MsaT028490.1:CDS |
AGTAATGCTCTCCTGTGTGT+CGG | 0.561176 | 5:+63098701 | None:intergenic |
TGGATGAAGTAGGAAGTTCA+AGG | 0.576606 | 5:-63098985 | MsaT028490.1:intron |
GGATGGATTAGGGATCCAAA+TGG | 0.577469 | 5:-63099155 | MsaT028490.1:CDS |
AAACTTATCACTTCAAATAG+GGG | 0.599416 | 5:-63098642 | MsaT028490.1:CDS |
GGATGAAGTAGGAAGTTCAA+GGG | 0.644562 | 5:-63098984 | MsaT028490.1:intron |
CAACTGCTTACCCGACACAC+AGG | 0.661269 | 5:-63098712 | MsaT028490.1:CDS |
TCCACAAAGAGACGATTGGG+AGG | 0.661851 | 5:+63098525 | None:intergenic |
AGTTGATAGATATCCTATTG+GGG | 0.667078 | 5:+63098730 | None:intergenic |
AAGGACCGTAGACTAAGGAA+AGG | 0.676015 | 5:-63098576 | MsaT028490.1:CDS |
AAACTATAATATCCTAACGA+CGG | 0.684003 | 5:+63098491 | None:intergenic |
AGATTGGCCCAAGTTCGTGA+AGG | 0.684958 | 5:-63098595 | MsaT028490.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr5 | gene | 63098494 | 63099228 | 63098494 | ID=MsaG028490 |
Chr5 | mRNA | 63098494 | 63099228 | 63098494 | ID=MsaT028490.1;Parent=MsaG028490 |
Chr5 | exon | 63098494 | 63098757 | 63098494 | ID=MsaT028490.1.exon3;Parent=MsaT028490.1 |
Chr5 | CDS | 63098494 | 63098757 | 63098494 | ID=cds.MsaT028490.1;Parent=MsaT028490.1 |
Chr5 | exon | 63098857 | 63098995 | 63098857 | ID=MsaT028490.1.exon2;Parent=MsaT028490.1 |
Chr5 | CDS | 63098857 | 63098995 | 63098857 | ID=cds.MsaT028490.1;Parent=MsaT028490.1 |
Chr5 | exon | 63099149 | 63099228 | 63099149 | ID=MsaT028490.1.exon1;Parent=MsaT028490.1 |
Chr5 | CDS | 63099149 | 63099228 | 63099149 | ID=cds.MsaT028490.1;Parent=MsaT028490.1 |
Gene Sequence |
Protein sequence |