AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028490 MtrunA17_Chr2g0281511 30.769 117 79 1 38 154 6 120 7.23e-16 69.7
MsaG028490 MtrunA17_Chr2g0309121 36.145 83 52 1 72 154 38 119 7.61e-15 67.0
MsaG028490 MtrunA17_Chr6g0459981 38.043 92 53 2 53 144 62 149 2.53e-14 66.6
MsaG028490 MtrunA17_Chr5g0430431 35.354 99 55 1 52 150 165 254 1.65e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAACTTATCACTTCAAAT+AGG 0.238418 5:-63098644 MsaT028490.1:CDS
AAACCGCAAAAGTATGATTA+TGG 0.295816 5:-63099176 MsaT028490.1:CDS
TCACCTAAGTATTTCTCATT+TGG 0.308885 5:+63098618 None:intergenic
CAAACTTATCACTTCAAATA+GGG 0.313955 5:-63098643 MsaT028490.1:CDS
TTGTTTAGCACTTCCCCAAT+AGG 0.356270 5:-63098743 MsaT028490.1:intron
CAGTTGATAGATATCCTATT+GGG 0.375059 5:+63098729 None:intergenic
TCTACGGTCCTTCACGAACT+TGG 0.378944 5:+63098587 None:intergenic
AGTATGATTATGGATGGATT+AGG 0.381484 5:-63099166 MsaT028490.1:CDS
ACAGGAGAGCATTACTTTGC+AGG 0.396717 5:-63098694 MsaT028490.1:CDS
ACTCATTACACAGTGCCATT+TGG 0.408378 5:+63099140 None:intergenic
TAAGTTTGCACTCATATTCT+CGG 0.417716 5:+63098658 None:intergenic
GCAGTTGATAGATATCCTAT+TGG 0.431455 5:+63098728 None:intergenic
GTATGATTATGGATGGATTA+GGG 0.435600 5:-63099165 MsaT028490.1:CDS
ACCTCCACAAAGAGACGATT+GGG 0.451863 5:+63098522 None:intergenic
AGAAATACTTAGGTGAAGAT+TGG 0.462766 5:-63098611 MsaT028490.1:CDS
TCCTCCCAATCGTCTCTTTG+TGG 0.465471 5:-63098526 MsaT028490.1:CDS
GTAATGCTCTCCTGTGTGTC+GGG 0.473972 5:+63098702 None:intergenic
AGGTTGTCGACGCCGTCGTT+AGG 0.482126 5:-63098503 MsaT028490.1:CDS
CGCAAAAGTATGATTATGGA+TGG 0.485297 5:-63099172 MsaT028490.1:CDS
ACATCTCTTGTTGTTAGCTG+TGG 0.496090 5:+63098866 None:intergenic
TATCACCTTTCCTTAGTCTA+CGG 0.505505 5:+63098571 None:intergenic
GGTCCAAATGAGAAATACTT+AGG 0.515401 5:-63098621 MsaT028490.1:CDS
TCGTGAAGGACCGTAGACTA+AGG 0.541590 5:-63098581 MsaT028490.1:CDS
AACCTCCACAAAGAGACGAT+TGG 0.547571 5:+63098521 None:intergenic
CAACAAGAGATGTCAAGTAA+TGG 0.551729 5:-63098856 MsaT028490.1:intron
CTACGGTCCTTCACGAACTT+GGG 0.555110 5:+63098588 None:intergenic
GTTCAAGGGAAATACAATCA+AGG 0.557132 5:-63098970 MsaT028490.1:CDS
TCCCAATCGTCTCTTTGTGG+AGG 0.560244 5:-63098523 MsaT028490.1:CDS
AGTAATGCTCTCCTGTGTGT+CGG 0.561176 5:+63098701 None:intergenic
TGGATGAAGTAGGAAGTTCA+AGG 0.576606 5:-63098985 MsaT028490.1:intron
GGATGGATTAGGGATCCAAA+TGG 0.577469 5:-63099155 MsaT028490.1:CDS
AAACTTATCACTTCAAATAG+GGG 0.599416 5:-63098642 MsaT028490.1:CDS
GGATGAAGTAGGAAGTTCAA+GGG 0.644562 5:-63098984 MsaT028490.1:intron
CAACTGCTTACCCGACACAC+AGG 0.661269 5:-63098712 MsaT028490.1:CDS
TCCACAAAGAGACGATTGGG+AGG 0.661851 5:+63098525 None:intergenic
AGTTGATAGATATCCTATTG+GGG 0.667078 5:+63098730 None:intergenic
AAGGACCGTAGACTAAGGAA+AGG 0.676015 5:-63098576 MsaT028490.1:CDS
AAACTATAATATCCTAACGA+CGG 0.684003 5:+63098491 None:intergenic
AGATTGGCCCAAGTTCGTGA+AGG 0.684958 5:-63098595 MsaT028490.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 63098494 63099228 63098494 ID=MsaG028490
Chr5 mRNA 63098494 63099228 63098494 ID=MsaT028490.1;Parent=MsaG028490
Chr5 exon 63098494 63098757 63098494 ID=MsaT028490.1.exon3;Parent=MsaT028490.1
Chr5 CDS 63098494 63098757 63098494 ID=cds.MsaT028490.1;Parent=MsaT028490.1
Chr5 exon 63098857 63098995 63098857 ID=MsaT028490.1.exon2;Parent=MsaT028490.1
Chr5 CDS 63098857 63098995 63098857 ID=cds.MsaT028490.1;Parent=MsaT028490.1
Chr5 exon 63099149 63099228 63099149 ID=MsaT028490.1.exon1;Parent=MsaT028490.1
Chr5 CDS 63099149 63099228 63099149 ID=cds.MsaT028490.1;Parent=MsaT028490.1
Gene Sequence

>MsaT028490.1

ATGTTCTCAGATTCTTCATCTTACTTCAAGAAACCGCAAAAGTATGATTATGGATGGATTAGGGATCCAAATGGCACTGTGAAGTTCAAGGGAAATACAATCAAGGAAGAAGTTATTGATTCTGATTCTGAAGATGATTGTGTAGAAATTGCTGATTATGATCATTTTGAGAGGAATGTCACTTATGCCACAGCTAACAACAAGAGATGTCAAGTAATGCACTTCCCCAATAGGATATCTATCAACTGCTTACCCGACACACAGGAGAGCATTACTTTGCAGGATGTGCATAATCTCCGAGAATATGAGTGCAAACTTATCACTTCAAATAGGGGTCCAAATGAGAAATACTTAGGTGAAGATTGGCCCAAGTTCGTGAAGGACCGTAGACTAAGGAAAGGTGATAAGTTGATATTTGATCTTTGTGCTCCTCCCAATCGTCTCTTTGTGGAGGTTGTCGACGCCGTCGTTAGGATATTATAG

Protein sequence

>MsaT028490.1

MFSDSSSYFKKPQKYDYGWIRDPNGTVKFKGNTIKEEVIDSDSEDDCVEIADYDHFERNVTYATANNKRCQVMHFPNRISINCLPDTQESITLQDVHNLREYECKLITSNRGPNEKYLGEDWPKFVKDRRLRKGDKLIFDLCAPPNRLFVEVVDAVVRIL*