AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028503


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028503 MtrunA17_Chr5g0432701 98.976 293 3 0 1 293 66 358 0.0 596
MsaG028503 MtrunA17_Chr3g0107491 70.336 327 61 6 1 293 53 377 1.98e-156 441
MsaG028503 MtrunA17_Chr3g0128781 54.745 274 109 5 26 293 47 311 1.23e-88 266
MsaG028503 MtrunA17_Chr2g0329551 55.140 214 52 5 24 193 107 320 5.74e-60 196
MsaG028503 MtrunA17_Chr6g0463571 60.571 175 55 6 35 203 95 261 1.12e-53 177
MsaG028503 MtrunA17_Chr4g0036071 73.684 114 30 0 79 192 345 458 5.43e-49 169
MsaG028503 MtrunA17_Chr1g0156051 56.944 144 52 2 59 192 342 485 1.21e-31 123
MsaG028503 MtrunA17_Chr2g0316551 51.064 94 46 0 93 186 207 300 1.51e-22 95.9
MsaG028503 MtrunA17_Chr2g0325131 39.869 153 79 4 94 243 191 333 1.09e-21 93.2
MsaG028503 MtrunA17_Chr4g0040891 48.387 93 48 0 94 186 194 286 1.38e-20 90.1
MsaG028503 MtrunA17_Chr1g0202051 41.818 110 62 2 79 187 109 217 6.32e-16 76.3
MsaG028503 MtrunA17_Chr2g0306581 56.364 55 24 0 85 139 4 58 2.85e-12 62.8
MsaG028503 MtrunA17_Chr8g0380611 40.426 94 51 1 93 186 233 321 9.46e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028503 AT2G40620.1 52.493 341 91 11 3 293 48 367 2.93e-91 275
MsaG028503 AT1G06850.1 57.621 269 87 7 29 282 70 326 3.62e-88 266
MsaG028503 AT2G31370.5 51.092 229 70 5 24 212 94 320 5.58e-62 201
MsaG028503 AT2G31370.2 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT2G31370.4 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT2G31370.1 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT2G31370.7 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT2G31370.3 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT2G31370.6 51.092 229 70 5 24 212 94 320 2.26e-61 200
MsaG028503 AT1G06070.1 52.358 212 67 4 24 202 108 318 9.13e-57 188
MsaG028503 AT4G38900.2 67.626 139 35 2 63 191 353 491 1.56e-49 172
MsaG028503 AT4G38900.3 67.626 139 35 2 63 191 353 491 1.56e-49 172
MsaG028503 AT1G43700.1 61.538 156 46 4 51 192 137 292 1.76e-48 165
MsaG028503 AT4G38900.1 64.828 145 35 3 63 191 353 497 5.13e-47 166
MsaG028503 AT2G21230.4 69.027 113 35 0 79 191 355 467 3.65e-43 154
MsaG028503 AT2G21230.1 69.027 113 35 0 79 191 355 467 3.65e-43 154
MsaG028503 AT1G06850.2 62.963 135 35 3 29 149 70 203 1.24e-42 146
MsaG028503 AT2G21230.3 64.706 119 36 1 79 191 355 473 3.75e-40 147
MsaG028503 AT2G21230.2 71.875 96 27 0 79 174 355 450 6.19e-37 137
MsaG028503 AT3G58120.1 49.462 93 47 0 94 186 202 294 1.09e-21 93.6
MsaG028503 AT2G42380.2 48.936 94 48 0 93 186 185 278 1.68e-21 92.8
MsaG028503 AT2G12900.1 43.269 104 58 1 86 189 109 211 4.47e-21 90.5
MsaG028503 AT2G13150.1 41.748 103 59 1 86 188 142 243 4.54e-18 82.4
MsaG028503 AT2G12940.1 39.423 104 62 1 85 188 140 242 1.46e-17 81.3
MsaG028503 AT2G42380.5 46.988 83 44 0 93 175 185 267 2.41e-17 80.5
MsaG028503 AT3G58120.2 47.500 80 42 0 94 173 202 281 2.53e-16 77.8
MsaG028503 AT2G42380.1 43.617 94 42 1 93 186 185 267 8.49e-15 73.9
MsaG028503 AT2G21235.1 34.722 144 62 3 94 205 359 502 1.15e-14 74.3
MsaG028503 AT1G58110.1 42.353 85 49 0 103 187 238 322 3.63e-12 66.6
MsaG028503 AT1G58110.2 42.353 85 49 0 103 187 238 322 3.63e-12 66.6

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATCCAGTTGCCACTATT+TGG 0.262115 5:+63257372 MsaT028503.1:CDS
AGCTATGCCTCCTGATAAAT+TGG 0.296982 5:+63254564 MsaT028503.1:CDS
GAGTTCGTTTGGGGAGATTA+TGG 0.335399 5:+63254531 MsaT028503.1:CDS
GTTGATGGGTCGAGTTCGTT+TGG 0.349009 5:+63254520 MsaT028503.1:CDS
ATTCCACCACAAGGTCCTTC+TGG 0.351170 5:+63257342 MsaT028503.1:CDS
TTCTGAATATGAATGAGAAT+TGG 0.357804 5:-63257436 None:intergenic
TTGATGGGTCGAGTTCGTTT+GGG 0.357989 5:+63254521 MsaT028503.1:CDS
GAAGGACCTTGTGGTGGAAT+TGG 0.359677 5:-63257339 None:intergenic
CAAAGCTCAGCCAATTTATC+AGG 0.368831 5:-63254574 None:intergenic
CTGATAAATTGGCTGAGCTT+TGG 0.380438 5:+63254575 MsaT028503.1:CDS
TGGAGATGGTGGAGCAGATC+CGG 0.389694 5:+63254438 MsaT028503.1:CDS
CGGCATAGTAATTCGGTTGA+TGG 0.408325 5:+63254505 MsaT028503.1:CDS
TGATGAGATGGCATGCTAGA+TGG 0.419162 5:-63257402 None:intergenic
TTGATTCTGTAAATTGCATT+TGG 0.421076 5:-63257308 None:intergenic
ATAGTGGCAACTGGATGTTC+CGG 0.448057 5:-63257368 None:intergenic
GTTGATGATCCGCCGGTGAA+TGG 0.450476 5:-63254349 None:intergenic
CGATGAGGCGGAGAAGAATC+CGG 0.451256 5:+63254468 MsaT028503.1:CDS
GAAAGAAGTTGAGAGATTGA+AGG 0.452522 5:+63257233 MsaT028503.1:CDS
GTCCCTGAAAACGACCAATC+TGG 0.466033 5:-63257473 None:intergenic
GAGGCATCGGCATAGTAATT+CGG 0.477726 5:+63254498 MsaT028503.1:CDS
TGCAGAAAGAGAAGGATCTT+CGG 0.478143 5:-63257532 None:intergenic
TCGTTTGGGGAGATTATGGA+TGG 0.478452 5:+63254535 MsaT028503.1:CDS
GTTGCTCCATGGCTTGCAGA+CGG 0.483654 5:-63257011 None:intergenic
AGTGACCAAATAGTGGCAAC+TGG 0.487180 5:-63257377 None:intergenic
AATATGAATGAGAATTGGTT+TGG 0.494880 5:-63257431 None:intergenic
GAGAGATTGAAGGTTGCTAC+TGG 0.498616 5:+63257243 MsaT028503.1:CDS
CGTTGAGAACAAGTCATCTT+CGG 0.499907 5:-63254395 None:intergenic
TTATTGTAGCGAGACACAAC+AGG 0.501264 5:+63256958 MsaT028503.1:intron
AGAAGAATCCGGTGAGGTCG+AGG 0.509126 5:+63254479 MsaT028503.1:CDS
ATCCGGTGAGGTCGAGGCAT+CGG 0.510001 5:+63254485 MsaT028503.1:CDS
TGCCCAACTGACACTATACC+AGG 0.512274 5:+63256540 MsaT028503.1:CDS
TGCTCGTTCAAAAGAGAGAA+AGG 0.513956 5:+63256453 MsaT028503.1:CDS
GAGTTGTAGCTTCAGTCTGT+AGG 0.522836 5:-63256513 None:intergenic
CACAAGGTCCTTCTGGTCAC+CGG 0.525022 5:+63257349 MsaT028503.1:CDS
TTCTAGTTCATGTATATAGC+GGG 0.529677 5:-63256476 None:intergenic
TACATTGACATGGATAAGCT+TGG 0.532268 5:+63254418 MsaT028503.1:CDS
GCCGGTGAATGGATCGGAAG+GGG 0.535786 5:-63254338 None:intergenic
GATGGGGAGTGACCAAATAG+TGG 0.537737 5:-63257384 None:intergenic
TGAATGGATCGGAAGGGGAG+AGG 0.537969 5:-63254333 None:intergenic
TCCCCTTCCGATCCATTCAC+CGG 0.539027 5:+63254337 MsaT028503.1:CDS
GAGATGTGCTTGTTGCTCCA+TGG 0.539738 5:-63257022 None:intergenic
AACCTGGTATAGTGTCAGTT+GGG 0.543208 5:-63256542 None:intergenic
GATGAGATGGCATGCTAGAT+GGG 0.545329 5:-63257401 None:intergenic
CCGCCGGTGAATGGATCGGA+AGG 0.545671 5:-63254340 None:intergenic
GGAGATGGTGGAGCAGATCC+GGG 0.548529 5:+63254439 MsaT028503.1:CDS
TTATTACAAAATGACCAGAT+TGG 0.549243 5:+63257459 MsaT028503.1:CDS
CTTTCACTTGCAGAAAGAGA+AGG 0.554279 5:-63257540 None:intergenic
AGTTGTAGCTTCAGTCTGTA+GGG 0.555319 5:-63256512 None:intergenic
AAACCTGGTATAGTGTCAGT+TGG 0.558403 5:-63256543 None:intergenic
TAAGCTCCGTCTGCAAGCCA+TGG 0.558627 5:+63257005 MsaT028503.1:CDS
GGCATAGTAATTCGGTTGAT+GGG 0.562173 5:+63254506 MsaT028503.1:CDS
GTTTGGTGCAGTTGATGAGA+TGG 0.569320 5:-63257414 None:intergenic
TTGATCCTAAGCGTGCCAAA+AGG 0.570345 5:+63254602 MsaT028503.1:CDS
TGATCCGCCGGTGAATGGAT+CGG 0.573063 5:-63254344 None:intergenic
ATGGATAAGCTTGGAGATGG+TGG 0.575379 5:+63254427 MsaT028503.1:CDS
ACGGCGAGCCTCGATGAGAT+CGG 0.578645 5:+63254370 MsaT028503.1:CDS
ATTCACCGGCGGATCATCAA+CGG 0.584726 5:+63254351 MsaT028503.1:CDS
TCCGCCTCATCGTTTCCACC+CGG 0.585072 5:-63254457 None:intergenic
TGCCGATGCCTCGACCTCAC+CGG 0.585516 5:-63254487 None:intergenic
GGACTTGACATTAGTAGCAA+AGG 0.593896 5:+63257492 MsaT028503.1:CDS
TGGATGTTCCGGTGACCAGA+AGG 0.596869 5:-63257357 None:intergenic
GCTCGCCGTTGATGATCCGC+CGG 0.602248 5:-63254356 None:intergenic
GTTCTAGTTCATGTATATAG+CGG 0.607918 5:-63256477 None:intergenic
CGTTTGGGGAGATTATGGAT+GGG 0.615032 5:+63254536 MsaT028503.1:CDS
AGCTCAGCCAATTTATCAGG+AGG 0.617494 5:-63254571 None:intergenic
CAACAAGCACATCTCCGTGA+TGG 0.633902 5:+63257030 MsaT028503.1:CDS
TGATGGGTCGAGTTCGTTTG+GGG 0.634252 5:+63254522 MsaT028503.1:CDS
GGTGATAATTGCTACCATCA+CGG 0.634943 5:-63257044 None:intergenic
TCCGGGTGGAAACGATGAGG+CGG 0.636862 5:+63254456 MsaT028503.1:CDS
GTTCTCAACGTACATTGACA+TGG 0.637215 5:+63254408 MsaT028503.1:CDS
GACATGGATAAGCTTGGAGA+TGG 0.645716 5:+63254424 MsaT028503.1:CDS
CGGTGACCAGAAGGACCTTG+TGG 0.645804 5:-63257348 None:intergenic
CGCCGGTGAATGGATCGGAA+GGG 0.655187 5:-63254339 None:intergenic
TGACCAGAAGGACCTTGTGG+TGG 0.657394 5:-63257345 None:intergenic
CTTCGGATCCGATCTCATCG+AGG 0.668290 5:-63254378 None:intergenic
GATGGTGGAGCAGATCCGGG+TGG 0.680229 5:+63254442 MsaT028503.1:CDS
TACGCTCCAATTCCACCACA+AGG 0.681715 5:+63257333 MsaT028503.1:CDS
ATGAGATGGCATGCTAGATG+GGG 0.693250 5:-63257400 None:intergenic
AGATCCGGGTGGAAACGATG+AGG 0.696064 5:+63254453 MsaT028503.1:CDS
CAAGATTAAAAGATTCAGTG+TGG 0.696933 5:-63257278 None:intergenic
CCTTCCGATCCATTCACCGG+CGG 0.698144 5:+63254340 MsaT028503.1:CDS
AGGCGGAGAAGAATCCGGTG+AGG 0.721189 5:+63254473 MsaT028503.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 63254325 63257578 63254325 ID=MsaG028503
Chr5 mRNA 63254325 63257578 63254325 ID=MsaT028503.1;Parent=MsaG028503
Chr5 exon 63254325 63254623 63254325 ID=MsaT028503.1.exon1;Parent=MsaT028503.1
Chr5 CDS 63254325 63254623 63254325 ID=cds.MsaT028503.1;Parent=MsaT028503.1
Chr5 exon 63256429 63256561 63256429 ID=MsaT028503.1.exon2;Parent=MsaT028503.1
Chr5 CDS 63256429 63256561 63256429 ID=cds.MsaT028503.1;Parent=MsaT028503.1
Chr5 exon 63256967 63257051 63256967 ID=MsaT028503.1.exon3;Parent=MsaT028503.1
Chr5 CDS 63256967 63257051 63256967 ID=cds.MsaT028503.1;Parent=MsaT028503.1
Chr5 exon 63257214 63257578 63257214 ID=MsaT028503.1.exon4;Parent=MsaT028503.1
Chr5 CDS 63257214 63257578 63257214 ID=cds.MsaT028503.1;Parent=MsaT028503.1
Gene Sequence

>MsaT028503.1

ATGATGGACCTCTCCCCTTCCGATCCATTCACCGGCGGATCATCAACGGCGAGCCTCGATGAGATCGGATCCGAAGATGACTTGTTCTCAACGTACATTGACATGGATAAGCTTGGAGATGGTGGAGCAGATCCGGGTGGAAACGATGAGGCGGAGAAGAATCCGGTGAGGTCGAGGCATCGGCATAGTAATTCGGTTGATGGGTCGAGTTCGTTTGGGGAGATTATGGATGGGAAGAAAGCTATGCCTCCTGATAAATTGGCTGAGCTTTGGTCTGTTGATCCTAAGCGTGCCAAAAGAATACTTGCTAATCGACAATCTGCTGCTCGTTCAAAAGAGAGAAAGGCCCGCTATATACATGAACTAGAACGCAAAGTTCAGACCCTACAGACTGAAGCTACAACTCTTTCTGCCCAACTGACACTATACCAGCGAGACACAACAGGTCTGAGTACTGAAAATACTGAGCTTAAGCTCCGTCTGCAAGCCATGGAGCAACAAGCACATCTCCGTGATGCTCTTAATGATGCATTGAAGAAAGAAGTTGAGAGATTGAAGGTTGCTACTGGAGATATAATGAGCCACACTGAATCTTTTAATCTTGGTATGCACCAAATGCAATTTACAGAATCAAATTACGCTCCAATTCCACCACAAGGTCCTTCTGGTCACCGGAACATCCAGTTGCCACTATTTGGTCACTCCCCATCTAGCATGCCATCTCATCAACTGCACCAAACCAATTCTCATTCATATTCAGAATTATTACAAAATGACCAGATTGGTCGTTTTCAGGGACTTGACATTAGTAGCAAAGGAACAGCTGTTGTAAAGTCCGAAGATCCTTCTCTTTCTGCAAGTGAAAGTAGCACAACATTTTGA

Protein sequence

>MsaT028503.1

MMDLSPSDPFTGGSSTASLDEIGSEDDLFSTYIDMDKLGDGGADPGGNDEAEKNPVRSRHRHSNSVDGSSSFGEIMDGKKAMPPDKLAELWSVDPKRAKRILANRQSAARSKERKARYIHELERKVQTLQTEATTLSAQLTLYQRDTTGLSTENTELKLRLQAMEQQAHLRDALNDALKKEVERLKVATGDIMSHTESFNLGMHQMQFTESNYAPIPPQGPSGHRNIQLPLFGHSPSSMPSHQLHQTNSHSYSELLQNDQIGRFQGLDISSKGTAVVKSEDPSLSASESSTTF*