AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028504


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028504 MtrunA17_Chr5g0432691 90.741 162 15 0 1 162 1 162 4.02e-112 315
MsaG028504 MtrunA17_Chr3g0107381 40.625 160 95 0 1 160 1 160 2.02e-44 145
MsaG028504 MtrunA17_Chr3g0107461 42.857 154 87 1 1 153 1 154 6.08e-41 135
MsaG028504 MtrunA17_Chr4g0014321 41.875 160 92 1 1 159 1 160 8.81e-41 136
MsaG028504 MtrunA17_Chr4g0014031 44.444 162 89 1 1 161 1 162 7.11e-40 134
MsaG028504 MtrunA17_Chr3g0110131 42.138 159 92 0 1 159 1 159 5.33e-39 132
MsaG028504 MtrunA17_Chr3g0107331 42.208 154 89 0 4 157 3 156 3.70e-37 125
MsaG028504 MtrunA17_Chr3g0107351 41.667 156 91 0 5 160 4 159 1.11e-36 124
MsaG028504 MtrunA17_Chr3g0107341 42.949 156 89 0 5 160 4 159 1.83e-36 123
MsaG028504 MtrunA17_Chr3g0107361 41.667 156 91 0 5 160 4 159 4.08e-36 122
MsaG028504 MtrunA17_Chr4g0013041 40.645 155 89 2 1 154 1 153 8.83e-35 120
MsaG028504 MtrunA17_Chr2g0283941 34.810 158 102 1 1 157 1 158 1.28e-34 121
MsaG028504 MtrunA17_Chr8g0351751 38.889 162 96 2 1 159 1 162 1.39e-34 121
MsaG028504 MtrunA17_Chr1g0188651 41.026 156 89 2 1 156 1 153 3.26e-34 117
MsaG028504 MtrunA17_Chr1g0188661 39.744 156 93 1 1 156 1 155 7.62e-34 117
MsaG028504 MtrunA17_Chr1g0188681 36.538 156 98 1 1 156 1 155 9.22e-33 114
MsaG028504 MtrunA17_Chr5g0420351 34.615 156 101 1 1 156 1 155 1.57e-31 110
MsaG028504 MtrunA17_Chr4g0014131 42.593 162 92 1 1 161 1 162 1.64e-31 113
MsaG028504 MtrunA17_Chr7g0218151 38.462 156 95 1 1 156 1 155 4.19e-31 109
MsaG028504 MtrunA17_Chr4g0014121 44.156 154 85 1 1 153 29 182 5.03e-31 110
MsaG028504 MtrunA17_Chr3g0105791 39.394 132 73 2 29 156 1 129 2.85e-30 107
MsaG028504 MtrunA17_Chr4g0012761 37.179 156 97 1 1 156 1 155 4.54e-30 107
MsaG028504 MtrunA17_Chr1g0197261 34.395 157 102 1 1 157 1 156 2.81e-29 105
MsaG028504 MtrunA17_Chr1g0193311 37.179 156 97 1 1 156 1 155 3.06e-29 105
MsaG028504 MtrunA17_Chr2g0296831 45.455 121 66 0 1 121 1 121 3.26e-29 103
MsaG028504 MtrunA17_Chr1g0197461 33.117 154 102 1 4 157 3 155 3.31e-28 102
MsaG028504 MtrunA17_Chr1g0197321 34.810 158 101 2 1 157 1 157 6.01e-27 99.4
MsaG028504 MtrunA17_Chr4g0031421 37.121 132 76 2 29 156 1 129 2.49e-26 97.1
MsaG028504 MtrunA17_Chr2g0296861 34.641 153 100 0 1 153 1 153 9.06e-26 96.3
MsaG028504 MtrunA17_Chr3g0128721 32.258 155 105 0 4 158 7 161 2.23e-23 95.5
MsaG028504 MtrunA17_Chr7g0267601 33.974 156 101 2 4 158 7 161 1.02e-22 93.6
MsaG028504 MtrunA17_Chr4g0008701 32.258 155 105 0 4 158 7 161 3.76e-22 92.0
MsaG028504 MtrunA17_Chr3g0106421 33.333 126 83 1 29 153 1 126 8.33e-22 87.4
MsaG028504 MtrunA17_Chr3g0106501 30.952 126 86 1 29 153 1 126 2.13e-17 74.3
MsaG028504 MtrunA17_Chr1g0212151 32.773 119 72 4 1 112 1 118 1.62e-14 69.7
MsaG028504 MtrunA17_Chr6g0457851 32.231 121 74 4 1 114 11 130 6.97e-14 67.8
MsaG028504 MtrunA17_Chr6g0449861 31.858 113 70 3 1 107 9 120 1.87e-11 60.8
MsaG028504 MtrunA17_Chr6g0449851 29.771 131 85 3 1 125 1 130 7.64e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028504 AT5G48670.1 38.562 153 94 0 1 153 1 153 2.05e-30 112
MsaG028504 AT1G65300.1 36.691 139 88 0 4 142 3 141 4.43e-27 103
MsaG028504 AT5G26650.1 34.615 156 102 0 4 159 2 157 1.65e-26 103
MsaG028504 AT1G65330.1 43.810 105 59 0 5 109 4 108 2.47e-26 100
MsaG028504 AT5G26630.1 33.962 159 105 0 1 159 1 159 5.85e-26 98.6
MsaG028504 AT3G05860.2 30.872 149 103 0 1 149 1 149 6.29e-26 98.2
MsaG028504 AT1G31630.1 32.692 156 104 1 4 159 3 157 1.62e-25 100
MsaG028504 AT5G27960.1 44.340 106 59 0 4 109 2 107 1.97e-25 99.4
MsaG028504 AT3G05860.3 30.872 149 103 0 1 149 1 149 5.20e-25 97.1
MsaG028504 AT3G05860.1 30.872 149 103 0 1 149 1 149 6.70e-25 97.1
MsaG028504 AT1G31640.1 33.553 152 92 4 5 152 4 150 9.63e-24 96.7
MsaG028504 AT5G27810.1 34.483 116 74 2 29 144 1 114 3.20e-20 81.3
MsaG028504 AT2G28700.1 34.862 109 71 0 1 109 1 109 6.39e-19 82.0
MsaG028504 AT5G26580.1 40.367 109 51 1 1 109 1 95 1.49e-18 81.3
MsaG028504 AT2G40210.1 40.594 101 60 0 1 101 1 101 5.74e-18 79.7
MsaG028504 AT5G06500.1 28.846 156 104 2 1 153 1 152 9.71e-16 72.4
MsaG028504 AT1G22590.2 30.435 115 80 0 1 115 1 115 1.49e-15 70.1
MsaG028504 AT2G15660.1 33.028 109 72 1 38 146 31 138 1.96e-12 63.5
MsaG028504 AT5G55690.1 36.283 113 58 4 1 105 1 107 5.69e-12 62.4
MsaG028504 AT5G55690.2 36.283 113 58 4 1 105 1 107 5.69e-12 62.4
MsaG028504 AT5G58890.1 38.182 110 61 2 1 104 1 109 8.19e-12 62.0

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTCTCATTCAACTTT+TGG 0.190821 5:-63260220 None:intergenic
ATGATCTTGAACCACAAATT+TGG 0.213404 5:+63260090 MsaT028504.1:CDS
TGCTCTCCTTGCTGTGTGAT+TGG 0.345198 5:-63259973 None:intergenic
TCTCATGGTTGAACATCTTT+CGG 0.372595 5:-63260178 None:intergenic
CTTCACTTTCTTTCCTGCCA+TGG 0.385906 5:-63259937 None:intergenic
ATTTGTGGTTCAAGATCATC+TGG 0.390617 5:-63260086 None:intergenic
TGATATGAAGATGGTGCAAT+TGG 0.408742 5:+63260385 MsaT028504.1:CDS
GGAGAGCAACGTACAAGAAA+AGG 0.412101 5:+63259988 MsaT028504.1:CDS
TGAACGGATGGCCAAATTTG+TGG 0.436771 5:-63260101 None:intergenic
TGAACCGAACCAGCACACTA+TGG 0.436948 5:-63260130 None:intergenic
GGAGAAGAATGAGAGGGCTT+AGG 0.440373 5:+63260406 MsaT028504.1:CDS
CAACCATGAGACTTACTTGA+AGG 0.443462 5:+63260190 MsaT028504.1:CDS
CAAATTTGGCCATCCGTTCA+AGG 0.457472 5:+63260104 MsaT028504.1:CDS
GAGAAGAATGAGAGGGCTTA+GGG 0.460909 5:+63260407 MsaT028504.1:CDS
TTTCTCAATACCACCATGGC+AGG 0.467282 5:+63259924 None:intergenic
GAGTCCATAGTGTGCTGGTT+CGG 0.472439 5:+63260126 MsaT028504.1:CDS
TTGCATCACTATCTTGAGGC+AGG 0.484072 5:+63260287 MsaT028504.1:CDS
ATGTGATTCATGAGAAAATG+AGG 0.503498 5:+63260357 MsaT028504.1:CDS
TCTGAAGTGCTTAAATCCTC+AGG 0.504006 5:-63260317 None:intergenic
GAGAGCAACGTACAAGAAAA+GGG 0.528840 5:+63259989 MsaT028504.1:CDS
ACATGCTTGAACTCCACATA+GGG 0.545908 5:-63260048 None:intergenic
TTTCCTTCAAGTAAGTCTCA+TGG 0.546071 5:-63260193 None:intergenic
TGCAATTGGAGAAGAATGAG+AGG 0.550028 5:+63260399 MsaT028504.1:CDS
CTATGGACTCCTTGAACGGA+TGG 0.558436 5:-63260113 None:intergenic
AATGAAATCACAACCCTATG+TGG 0.562718 5:+63260035 MsaT028504.1:CDS
CACACTATGGACTCCTTGAA+CGG 0.564303 5:-63260117 None:intergenic
ATGAGACTTACTTGAAGGAA+AGG 0.565276 5:+63260195 MsaT028504.1:CDS
TCAAGGAGTCCATAGTGTGC+TGG 0.565483 5:+63260121 MsaT028504.1:CDS
GAGGGAAATTGATATGAAGA+TGG 0.575041 5:+63260376 MsaT028504.1:CDS
TGAAGGAGAATAGGATGATG+TGG 0.587839 5:+63260252 MsaT028504.1:CDS
TGCATCACTATCTTGAGGCA+GGG 0.606928 5:+63260288 MsaT028504.1:CDS
CACATGCTTGAACTCCACAT+AGG 0.630810 5:-63260049 None:intergenic
TCAATTGCATCACTATCTTG+AGG 0.632807 5:+63260283 MsaT028504.1:CDS
TGTGATTCATGAGAAAATGA+GGG 0.634781 5:+63260358 MsaT028504.1:CDS
TGAGGCAGGGAATGTTCCTG+AGG 0.646378 5:+63260301 MsaT028504.1:CDS
TCATAACCAATCACACAGCA+AGG 0.650603 5:+63259967 MsaT028504.1:CDS
CTATGTGGAGTTCAAGCATG+TGG 0.662987 5:+63260050 MsaT028504.1:CDS
GCAATTGGAGAAGAATGAGA+GGG 0.674364 5:+63260400 MsaT028504.1:CDS
GGTTCGGTTCATGCATACTG+CGG 0.703185 5:+63260142 MsaT028504.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 63259939 63260427 63259939 ID=MsaG028504
Chr5 mRNA 63259939 63260427 63259939 ID=MsaT028504.1;Parent=MsaG028504
Chr5 exon 63259939 63260427 63259939 ID=MsaT028504.1.exon1;Parent=MsaT028504.1
Chr5 CDS 63259939 63260427 63259939 ID=cds.MsaT028504.1;Parent=MsaT028504.1
Gene Sequence

>MsaT028504.1

ATGGCAGGAAAGAAAGTGAAGCTTGCATTCATAACCAATCACACAGCAAGGAGAGCAACGTACAAGAAAAGGGTACAAAGTTTGATGAAGAAGCTCAATGAAATCACAACCCTATGTGGAGTTCAAGCATGTGGTATAGTTTTCAAGCCAGATGATCTTGAACCACAAATTTGGCCATCCGTTCAAGGAGTCCATAGTGTGCTGGTTCGGTTCATGCATACTGCGGACTTCGATCGAGACCGAAAGATGTTCAACCATGAGACTTACTTGAAGGAAAGGATCCAAAAGTTGAATGAGAAGTTGAAGAAAAAAGTGAAGGAGAATAGGATGATGTGGATGAGTGTTCAATTGCATCACTATCTTGAGGCAGGGAATGTTCCTGAGGATTTAAGCACTTCAGATATGAATGATCTCACATATGTGATTCATGAGAAAATGAGGGAAATTGATATGAAGATGGTGCAATTGGAGAAGAATGAGAGGGCTTAG

Protein sequence

>MsaT028504.1

MAGKKVKLAFITNHTARRATYKKRVQSLMKKLNEITTLCGVQACGIVFKPDDLEPQIWPSVQGVHSVLVRFMHTADFDRDRKMFNHETYLKERIQKLNEKLKKKVKENRMMWMSVQLHHYLEAGNVPEDLSTSDMNDLTYVIHEKMREIDMKMVQLEKNERA*