AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028584 MtrunA17_Chr5g0431811 85.993 307 23 1 1 287 242 548 3.68e-180 540
MsaG028584 MtrunA17_Chr5g0431811 61.303 261 54 9 285 521 601 838 1.94e-81 278
MsaG028584 MtrunA17_Chr5g0431811 73.006 163 41 1 414 573 810 972 1.71e-66 236
MsaG028584 MtrunA17_Chr5g0431731 57.877 292 83 3 39 292 194 483 5.65e-98 313
MsaG028584 MtrunA17_Chr5g0431731 34.286 140 73 1 453 573 396 535 4.58e-16 82.0
MsaG028584 MtrunA17_Chr5g0431761 50.000 330 124 3 1 291 214 541 2.64e-97 310
MsaG028584 MtrunA17_Chr5g0431871 79.444 180 17 1 46 205 1 180 4.82e-95 290
MsaG028584 MtrunA17_Chr5g0431741 46.483 327 133 4 1 287 213 537 5.50e-84 278
MsaG028584 MtrunA17_Chr3g0106431 53.220 295 95 4 38 291 183 475 1.85e-82 272
MsaG028584 MtrunA17_Chr3g0106431 33.571 140 74 1 453 573 389 528 1.21e-15 80.5
MsaG028584 MtrunA17_Chr5g0431881 84.252 127 20 0 447 573 1 127 9.87e-70 226
MsaG028584 MtrunA17_Chr5g0432041 39.365 315 159 5 2 287 209 520 2.35e-59 211
MsaG028584 MtrunA17_Chr5g0432041 40.458 131 66 2 447 565 439 569 2.98e-21 98.6
MsaG028584 MtrunA17_Chr5g0432091 36.795 337 160 6 1 287 213 546 1.81e-55 201
MsaG028584 MtrunA17_Chr3g0104851 35.922 309 152 8 15 283 210 512 3.34e-41 159
MsaG028584 MtrunA17_Chr3g0104851 38.028 142 73 3 447 573 434 575 2.47e-17 85.9
MsaG028584 MtrunA17_Chr4g0057561 30.717 293 162 4 34 287 236 526 8.58e-40 155
MsaG028584 MtrunA17_Chr5g0431891 40.426 235 75 4 1 215 85 274 2.93e-38 143
MsaG028584 MtrunA17_Chr5g0431821 40.426 235 75 4 1 215 231 420 2.54e-37 144
MsaG028584 MtrunA17_Chr1g0177671 32.342 269 153 3 51 292 204 470 4.04e-37 147
MsaG028584 MtrunA17_Chr1g0197101 31.227 269 156 3 51 292 210 476 1.70e-36 145
MsaG028584 MtrunA17_Chr1g0197101 31.783 129 80 2 449 569 396 524 1.25e-11 67.8
MsaG028584 MtrunA17_Chr8g0344461 34.199 231 129 3 46 255 161 389 1.95e-35 141
MsaG028584 MtrunA17_Chr6g0462011 30.945 307 162 4 33 291 269 573 2.76e-34 139
MsaG028584 MtrunA17_Chr5g0431991 39.640 222 103 4 1 205 209 416 7.71e-34 134
MsaG028584 MtrunA17_Chr5g0431901 82.895 76 13 0 1 76 65 140 8.25e-34 125
MsaG028584 MtrunA17_Chr7g0219041 29.831 295 151 5 45 287 225 515 3.82e-33 135
MsaG028584 MtrunA17_Chr3g0086881 28.723 282 159 3 46 287 181 460 1.68e-31 130
MsaG028584 MtrunA17_Chr7g0224041 29.553 291 156 6 52 296 216 503 9.97e-30 124
MsaG028584 MtrunA17_Chr4g0030621 30.088 226 135 3 53 257 177 400 4.29e-29 122
MsaG028584 MtrunA17_Chr2g0327741 27.848 237 147 3 43 257 154 388 1.57e-28 120
MsaG028584 MtrunA17_Chr1g0182041 29.412 238 134 6 46 257 166 395 2.57e-26 114
MsaG028584 MtrunA17_Chr5g0431981 37.278 169 95 3 1 169 210 367 2.89e-25 108
MsaG028584 MtrunA17_Chr1g0182031 28.151 238 137 6 46 257 164 393 3.14e-25 110
MsaG028584 MtrunA17_Chr5g0444661 40.288 139 71 2 447 573 253 391 2.34e-24 106
MsaG028584 MtrunA17_Chr5g0444661 35.260 173 83 3 144 287 162 334 7.63e-18 86.7
MsaG028584 MtrunA17_Chr5g0431971 34.868 152 87 2 444 583 92 243 6.93e-22 97.4
MsaG028584 MtrunA17_Chr5g0431801 58.427 89 35 1 2 90 214 300 8.94e-22 96.7
MsaG028584 MtrunA17_Chr1g0214001 31.356 236 136 3 45 256 197 430 2.16e-21 99.0
MsaG028584 MtrunA17_Chr5g0431851 60.274 73 27 1 2 74 212 282 5.01e-16 79.0
MsaG028584 MtrunA17_Chr3g0126391 41.892 74 42 1 343 415 125 198 1.24e-14 75.9
MsaG028584 MtrunA17_Chr8g0387981 48.387 62 31 1 349 409 130 191 2.01e-12 69.3
MsaG028584 MtrunA17_Chr3g0128671 41.176 68 39 1 352 418 128 195 9.69e-12 66.6
MsaG028584 MtrunA17_Chr7g0244971 43.750 64 35 1 349 411 121 184 1.31e-11 65.9
MsaG028584 MtrunA17_Chr7g0244981 42.424 66 37 1 347 411 118 183 1.98e-11 65.5
MsaG028584 MtrunA17_Chr8g0347251 37.234 94 57 2 343 434 128 221 6.36e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028584 AT5G58430.1 55.830 283 94 3 39 292 189 469 2.22e-93 301
MsaG028584 AT5G58430.1 35.507 138 78 3 453 579 391 528 9.82e-12 68.6
MsaG028584 AT1G07000.1 42.606 284 130 4 39 291 171 452 2.56e-62 218
MsaG028584 AT1G07000.1 33.077 130 77 2 458 577 380 509 2.75e-13 73.6
MsaG028584 AT5G50380.1 33.217 286 157 3 34 287 223 506 5.29e-45 171
MsaG028584 AT5G03540.2 35.135 259 138 3 57 287 95 351 2.80e-42 160
MsaG028584 AT5G03540.2 30.000 130 82 2 449 569 275 404 2.86e-12 70.1
MsaG028584 AT5G03540.1 35.135 259 138 3 57 287 210 466 8.20e-42 161
MsaG028584 AT5G03540.1 30.000 130 82 2 449 569 390 519 3.10e-12 70.1
MsaG028584 AT5G03540.3 35.135 259 138 3 57 287 236 492 2.59e-41 160
MsaG028584 AT5G03540.3 30.000 130 82 2 449 569 416 545 3.25e-12 70.1
MsaG028584 AT1G54090.1 32.753 287 144 5 46 287 170 452 3.44e-37 147
MsaG028584 AT3G14090.1 30.450 289 157 4 46 292 173 459 5.40e-36 144
MsaG028584 AT5G52340.1 30.855 269 157 3 51 292 204 470 6.47e-35 140
MsaG028584 AT5G13990.1 33.000 300 158 5 32 292 236 531 3.12e-33 136
MsaG028584 AT1G72470.1 32.432 222 126 3 52 251 184 403 3.79e-31 129
MsaG028584 AT5G13150.1 30.690 290 153 5 49 292 207 494 1.09e-29 125
MsaG028584 AT3G29400.1 27.243 301 161 4 43 287 194 492 3.57e-28 120
MsaG028584 AT5G59730.1 29.870 231 139 5 52 261 165 393 1.44e-26 115
MsaG028584 AT5G59730.2 29.870 231 139 5 52 261 165 393 1.73e-26 115
MsaG028584 AT5G52350.1 27.797 295 178 4 49 310 156 448 3.27e-25 110
MsaG028584 AT3G55150.1 25.714 245 160 3 53 277 183 425 1.58e-23 105
MsaG028584 AT2G40740.1 39.716 141 74 5 318 454 142 275 3.45e-23 100
MsaG028584 AT2G40740.3 39.716 141 74 5 318 454 151 284 4.10e-23 100
MsaG028584 AT2G39380.1 26.383 235 151 2 53 267 182 414 4.23e-23 104
MsaG028584 AT1G07725.1 27.948 229 141 3 52 260 175 399 1.05e-22 103
MsaG028584 AT5G61010.1 25.623 281 167 4 47 287 205 483 1.23e-21 100
MsaG028584 AT5G61010.2 25.623 281 167 4 47 287 205 483 1.23e-21 100
MsaG028584 AT3G09520.1 25.103 243 156 5 40 258 158 398 3.21e-21 98.6
MsaG028584 AT2G28640.1 25.974 231 149 3 50 260 153 381 1.34e-19 93.6
MsaG028584 AT3G09530.1 27.751 209 126 3 56 241 169 375 3.90e-19 92.0
MsaG028584 AT2G28650.1 23.970 267 165 5 57 287 152 416 8.98e-16 81.3
MsaG028584 AT2G46400.1 37.615 109 58 4 312 416 67 169 6.03e-14 73.6
MsaG028584 AT4G11070.2 40.625 96 52 2 322 416 84 175 4.19e-13 70.9
MsaG028584 AT4G11070.1 40.625 96 52 2 322 416 116 207 5.37e-13 70.9
MsaG028584 AT4G23810.1 40.426 94 51 2 322 414 133 222 5.97e-13 70.9
MsaG028584 AT3G56400.1 26.842 190 104 6 249 416 10 186 9.41e-13 69.7
MsaG028584 AT2G40740.2 38.636 88 46 2 370 454 149 231 3.75e-12 67.4
MsaG028584 AT1G66560.1 44.444 63 32 2 350 410 102 163 1.50e-11 65.5
MsaG028584 AT5G22570.1 39.706 68 40 1 352 418 111 178 1.99e-11 65.9
MsaG028584 AT1G66550.2 42.188 64 36 1 348 410 100 163 2.33e-11 65.1
MsaG028584 AT1G66550.1 42.188 64 36 1 348 410 105 168 2.65e-11 64.7
MsaG028584 AT5G24110.1 38.272 81 43 2 351 430 113 187 7.04e-11 64.3

Find 161 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGGTCGTTAATGGTTTC+TGG 0.108762 5:+64446774 None:intergenic
TTAGGGTCGGTTAAAATTTC+TGG 0.135438 5:+64433082 None:intergenic
TCCTATGATGGTGAAAATTT+TGG 0.157202 5:-64440304 MsaT028584.1:CDS
GGCCTCTGGCATAGAGTTTA+TGG 0.171782 5:+64440359 None:intergenic
TCTTGCTAAGGTTGGGGTTT+TGG 0.173248 5:+64433055 None:intergenic
AACAATTAGGGACATATTTA+AGG 0.175778 5:-64446256 MsaT028584.1:CDS
CTGACAAAGGCGACAAATTT+TGG 0.195810 5:-64446207 MsaT028584.1:CDS
TTCAAAGATTCGTAGAATTA+TGG 0.204532 5:-64446082 MsaT028584.1:CDS
CTTTGTGACCGCATCTTCTT+TGG 0.206023 5:-64446516 MsaT028584.1:CDS
TTCATCACATGTTGGGTAAT+TGG 0.206411 5:+64446171 None:intergenic
GGAAAGAACAAGAGATTAAA+AGG 0.215882 5:-64433225 MsaT028584.1:CDS
CTACTTCACAACTTTATATT+CGG 0.225913 5:-64441195 MsaT028584.1:CDS
CAGCAGATCAAAGTTGTTAA+TGG 0.265667 5:-64440255 MsaT028584.1:CDS
CGCGGCTGATTTCTCCTTCA+TGG 0.272027 5:-64446484 MsaT028584.1:CDS
AGAGACTGGGGAAAACAATT+AGG 0.294854 5:-64446269 MsaT028584.1:CDS
TAATGGCAGGAGAAAGAAAT+TGG 0.297328 5:-64444112 MsaT028584.1:intron
TGCCTAATGCATAAATTATT+CGG 0.304737 5:-64446663 MsaT028584.1:CDS
TATTTCCTCAAGGTCGTTAA+TGG 0.309182 5:+64446767 None:intergenic
GGATACATTGGAGAGCAATT+TGG 0.317424 5:-64446061 MsaT028584.1:CDS
AAGTTGTTGTACTTTCTTCC+TGG 0.317889 5:+64441318 None:intergenic
ATGCTTGTCTTGCTAAGGTT+GGG 0.321958 5:+64433048 None:intergenic
GCTTCAACGCATCCATCATT+AGG 0.327456 5:+64446796 None:intergenic
TGCTACGAGGACTCTTCTTT+AGG 0.341531 5:-64446024 MsaT028584.1:intron
CCGATGTGTACAACAATTGC+CGG 0.343817 5:-64446704 MsaT028584.1:CDS
TATATAACTTCAAATATGTT+TGG 0.345904 5:+64441286 None:intergenic
AACGCATCCATCATTAGGTT+TGG 0.350044 5:+64446801 None:intergenic
CACCGGAAGACGGGTTTATC+TGG 0.355975 5:-64444026 MsaT028584.1:CDS
GGAATGAAGCAACTGCTATA+TGG 0.360678 5:-64446293 MsaT028584.1:CDS
TGTGTTCCCAAACTGGGGTT+CGG 0.363263 5:+64444059 None:intergenic
ATCTCTCCCGAACCCCAGTT+TGG 0.364203 5:-64444066 MsaT028584.1:CDS
AGACTTGCTCTAATGTTTGC+TGG 0.368138 5:+64446124 None:intergenic
AGCCGAATAATTTATGCATT+AGG 0.379805 5:+64446661 None:intergenic
GGGTTTATCTGGAAAGAATA+TGG 0.380541 5:-64444015 MsaT028584.1:CDS
TCATCACATGTTGGGTAATT+GGG 0.383234 5:+64446172 None:intergenic
ATCCATTGATGGTATTGTTA+AGG 0.387780 5:+64441237 None:intergenic
CCCCGCCATCATTTCTTGAC+CGG 0.393350 5:+64445823 None:intergenic
AGCATGTTAGGTGGTTGTTT+AGG 0.403370 5:+64433178 None:intergenic
GTGTTCCCAAACTGGGGTTC+GGG 0.408159 5:+64444060 None:intergenic
GACAAATAGAACAAGTGAAA+TGG 0.408450 5:-64440097 MsaT028584.1:CDS
TAGGGACATATTTAAGGAGT+TGG 0.415790 5:-64446250 MsaT028584.1:CDS
TACTTCACAACTTTATATTC+GGG 0.417015 5:-64441194 MsaT028584.1:CDS
GAAAGAACAAGAGATTAAAA+GGG 0.418982 5:-64433224 MsaT028584.1:CDS
AAGGAGTTGGAGTATTTGAT+TGG 0.419385 5:-64446237 MsaT028584.1:CDS
TTCCAGATAAACCCGTCTTC+CGG 0.427566 5:+64444024 None:intergenic
TTTGTATGTGTTCCCAAACT+GGG 0.440270 5:+64444053 None:intergenic
TCTCTCCCGAACCCCAGTTT+GGG 0.442895 5:-64444065 MsaT028584.1:CDS
GCAAAGGTCATGTGTATGGC+TGG 0.445054 5:-64446744 MsaT028584.1:CDS
TCACAAAGTCTTCGCTCGCC+AGG 0.445872 5:+64446531 None:intergenic
TGCTTGTCTTGCTAAGGTTG+GGG 0.446215 5:+64433049 None:intergenic
AACAAAGTTTAGAAGTTGAA+TGG 0.450144 5:+64446443 None:intergenic
TGCTATAGTTCCTACTGTTG+TGG 0.450223 5:+64433143 None:intergenic
CGAATATAAAGTTGTGAAGT+AGG 0.451004 5:+64441196 None:intergenic
TATATTCGGGTGGATTTCAT+CGG 0.457601 5:-64441181 MsaT028584.1:CDS
GTTTGAGTAACATCCATTGA+TGG 0.463141 5:+64441226 None:intergenic
CATTGAAGAACACTCTCAAT+TGG 0.465502 5:-64446835 MsaT028584.1:CDS
TTTGTTTCAGGAGTTACTAC+AGG 0.465753 5:-64441372 MsaT028584.1:intron
GTCCTTAACAATACCATCAA+TGG 0.466957 5:-64441239 MsaT028584.1:CDS
CAATTGCCGGAGGGAATGCT+TGG 0.469099 5:-64446691 MsaT028584.1:CDS
TCAAAGAAACTCATGGGATA+AGG 0.472256 5:-64440007 MsaT028584.1:intron
AACTGCTATATGGAAGAGAC+TGG 0.473001 5:-64446283 MsaT028584.1:CDS
AATTATATGCGTGTCGAGCC+AGG 0.475350 5:-64441336 MsaT028584.1:CDS
TTGAAGACAAGATTGAAAGA+TGG 0.477159 5:-64446590 MsaT028584.1:CDS
GCTCTAATGTTTGCTGGGAT+CGG 0.478395 5:+64446130 None:intergenic
TGTTCTTTCCACTGCCCTTG+TGG 0.486496 5:+64433238 None:intergenic
TTAGCTGCTCCGACGGATGG+TGG 0.488050 5:-64440333 MsaT028584.1:CDS
AACTCTATCAAAGAAACTCA+TGG 0.490285 5:-64440014 MsaT028584.1:CDS
CTCTGGTGAGTGACTTCCAC+TGG 0.491333 5:+64446410 None:intergenic
GACTTGCTCTAATGTTTGCT+GGG 0.491728 5:+64446125 None:intergenic
ATATTCGGGTGGATTTCATC+GGG 0.493174 5:-64441180 MsaT028584.1:CDS
AATGCTTGTCTTGCTAAGGT+TGG 0.496098 5:+64433047 None:intergenic
GTGGATTTCATCGGGCATAC+TGG 0.496521 5:-64441172 MsaT028584.1:intron
GAGACTGGGGAAAACAATTA+GGG 0.498215 5:-64446268 MsaT028584.1:CDS
TACAAAATGCCACCGGAAGA+CGG 0.501523 5:-64444036 MsaT028584.1:CDS
CTCCTTCATGGAGATTTGCA+AGG 0.507882 5:-64446472 MsaT028584.1:CDS
GAGGTAGTTCATCACATGTT+GGG 0.509089 5:+64446164 None:intergenic
CACCCTCGCAACCAGGAGAA+AGG 0.510246 5:-64445752 MsaT028584.1:intron
AGAAGGGTATGCATATTCTT+CGG 0.510856 5:+64440164 None:intergenic
ACAAAATGCCACCGGAAGAC+GGG 0.511101 5:-64444035 MsaT028584.1:CDS
ATGTTAGCTGCTCCGACGGA+TGG 0.511981 5:-64440336 MsaT028584.1:CDS
TCCGGTCAAGAAATGATGGC+GGG 0.512447 5:-64445824 MsaT028584.1:CDS
AATAGCAAAGGTCATGTGTA+TGG 0.513892 5:-64446748 MsaT028584.1:CDS
AAGAGATTAAAAGGGGAAGA+AGG 0.517347 5:-64433216 MsaT028584.1:CDS
TACCTTTCTCCTGGTTGCGA+GGG 0.522243 5:+64445750 None:intergenic
TCAAGAAATGATGGCGGGGA+AGG 0.522448 5:-64445819 MsaT028584.1:CDS
GGAGCAGCTAACATGGCCTC+TGG 0.529383 5:+64440345 None:intergenic
AGAACAAACCACAAGGGCAG+TGG 0.531911 5:-64433246 MsaT028584.1:CDS
ATACCTTTCTCCTGGTTGCG+AGG 0.537516 5:+64445749 None:intergenic
AAATACCTCGGGTTGAAGCC+AGG 0.538543 5:+64443979 None:intergenic
TAACTTCAAATATGTTTGGA+TGG 0.540945 5:+64441290 None:intergenic
TCTTATAGCAGAAACAAGAT+AGG 0.542847 5:+64440281 None:intergenic
GGCCATGTTAGCTGCTCCGA+CGG 0.545472 5:-64440340 MsaT028584.1:CDS
ACAGCTAATAGTATTCAAGA+AGG 0.546113 5:-64433269 MsaT028584.1:intron
ATCAAAGTTGTTAATGGAGA+CGG 0.551909 5:-64440249 MsaT028584.1:CDS
CTTACAGAAGCTCAGTATTG+AGG 0.553708 5:-64446640 MsaT028584.1:CDS
GGGAAGAAGGCATCCCACGC+AGG 0.557330 5:-64433203 MsaT028584.1:CDS
GGAGGTAGTTCATCACATGT+TGG 0.559186 5:+64446163 None:intergenic
TCGTAGAATTATGGATACAT+TGG 0.561410 5:-64446073 MsaT028584.1:CDS
GCTGGGATCGGCAAACTCCA+TGG 0.562925 5:+64446142 None:intergenic
GAAGGATCAAAGGTGTATCA+AGG 0.565861 5:-64445801 MsaT028584.1:CDS
AAAGTTTAGAAGTTGAATGG+TGG 0.567993 5:+64446446 None:intergenic
TAGCTGCTCCGACGGATGGT+GGG 0.569296 5:-64440332 MsaT028584.1:CDS
TTCCTTGCAAATCTCCATGA+AGG 0.570776 5:+64446470 None:intergenic
TGACCTTTGCTATTTCCTCA+AGG 0.571227 5:+64446757 None:intergenic
AGAAAACAAACCACAACAGT+AGG 0.571849 5:-64433153 MsaT028584.1:CDS
GATCGATCCAAACCTAATGA+TGG 0.572122 5:-64446808 MsaT028584.1:CDS
ATCCGTCGGAGCAGCTAACA+TGG 0.572394 5:+64440338 None:intergenic
AATACAAACTGAGGGGACAA+TGG 0.572663 5:-64440217 MsaT028584.1:CDS
TGATGGCGGGGAAGGATCAA+AGG 0.572892 5:-64445811 MsaT028584.1:CDS
TTCACAACTTTATATTCGGG+TGG 0.575292 5:-64441191 MsaT028584.1:CDS
CAAAGGTCATGTGTATGGCT+GGG 0.578961 5:-64446743 MsaT028584.1:CDS
GAAGTATCACCCTCGCAACC+AGG 0.581020 5:-64445759 MsaT028584.1:CDS
CACAAAGTCTTCGCTCGCCA+GGG 0.584462 5:+64446532 None:intergenic
ATAAAGTTGTGAAGTAGGCA+TGG 0.586430 5:+64441201 None:intergenic
CAGATAAACCCGTCTTCCGG+TGG 0.589083 5:+64444027 None:intergenic
AAAGAACAAGAGATTAAAAG+GGG 0.593074 5:-64433223 MsaT028584.1:CDS
AGGACGTTCACAATATGTCA+TGG 0.594715 5:-64446620 MsaT028584.1:CDS
TGAAGAACACTCTCAATTGG+TGG 0.597010 5:-64446832 MsaT028584.1:CDS
ACTCTATCAAAGAAACTCAT+GGG 0.597218 5:-64440013 MsaT028584.1:CDS
TATTGTTAAGGACATGCGGC+AGG 0.597318 5:+64441249 None:intergenic
ACTTGTCCAAGCATTCCCTC+CGG 0.597670 5:+64446685 None:intergenic
TTATCTGGAAAGAATATGGA+AGG 0.599335 5:-64444011 MsaT028584.1:CDS
TTGTATGTGTTCCCAAACTG+GGG 0.599512 5:+64444054 None:intergenic
GTGTATGGCTGGGTTTGAGA+AGG 0.604710 5:-64446733 MsaT028584.1:CDS
ACGAATCTTTGAAGAAAGTG+AGG 0.606278 5:+64446092 None:intergenic
CGTTCACAATATGTCATGGA+AGG 0.608710 5:-64446616 MsaT028584.1:CDS
AGGAAGAAAGTACAACAACT+TGG 0.610203 5:-64441316 MsaT028584.1:CDS
CTTCTTTGGATTCTCATCCG+CGG 0.620030 5:-64446502 MsaT028584.1:CDS
CATGTGATGAACTACCTCCA+TGG 0.620759 5:-64446159 MsaT028584.1:CDS
TGGTGGGCATCATCCTATGA+TGG 0.624602 5:-64440316 MsaT028584.1:CDS
CGACCTTGAGGAAATAGCAA+AGG 0.625252 5:-64446760 MsaT028584.1:CDS
GGTAGTATGATGGAACAACT+CGG 0.625463 5:-64446930 None:intergenic
GCGTTTGTTTAAAATCCTTG+AGG 0.626854 5:-64446388 MsaT028584.1:CDS
GACACGCATATAATTTCATG+TGG 0.627518 5:+64441344 None:intergenic
TTCCGGTCAAGAAATGATGG+CGG 0.631799 5:-64445825 MsaT028584.1:CDS
AGATACCTGGCTTCAACCCG+AGG 0.632242 5:-64443984 MsaT028584.1:intron
GTAGTATGATGGAACAACTC+GGG 0.634681 5:-64446929 None:intergenic
ATGGTATTGTTAAGGACATG+CGG 0.637436 5:+64441245 None:intergenic
ACTGCTATATGGAAGAGACT+GGG 0.640080 5:-64446282 MsaT028584.1:CDS
GGTTGTTTAGGTGCCTGCGT+GGG 0.644999 5:+64433190 None:intergenic
ATCCATAAACTCTATGCCAG+AGG 0.646131 5:-64440361 MsaT028584.1:CDS
TTTGTTGATTATGTGTCCAG+TGG 0.647337 5:-64446426 MsaT028584.1:CDS
GGATGATGCCCACCATCCGT+CGG 0.648793 5:+64440324 None:intergenic
ACAACTCGGGACTTATGTGA+AGG 0.656137 5:-64446916 MsaT028584.1:CDS
TATGGAAGGAAAGAGATACC+TGG 0.657804 5:-64443997 MsaT028584.1:CDS
TGGTTGTTTAGGTGCCTGCG+TGG 0.658253 5:+64433189 None:intergenic
CTGCTATATGGAAGAGACTG+GGG 0.659434 5:-64446281 MsaT028584.1:CDS
CCGGCAATTGTTGTACACAT+CGG 0.663591 5:+64446704 None:intergenic
ATGAGAATCCAAAGAAGATG+CGG 0.664736 5:+64446508 None:intergenic
CCGGTCAAGAAATGATGGCG+GGG 0.666039 5:-64445823 MsaT028584.1:CDS
ATGTGTACAACAATTGCCGG+AGG 0.668455 5:-64446701 MsaT028584.1:CDS
TTTGAAGTTATATACAACGG+TGG 0.672572 5:-64441277 MsaT028584.1:CDS
GAACACATACAAAATGCCAC+CGG 0.673182 5:-64444043 MsaT028584.1:CDS
AAGAAGGAGAACAAACCACA+AGG 0.677055 5:-64433253 MsaT028584.1:CDS
ATATTTGAAGTTATATACAA+CGG 0.679230 5:-64441280 MsaT028584.1:CDS
AGAAACCATTAACGACCTTG+AGG 0.681166 5:-64446772 MsaT028584.1:CDS
AAGTTGTGAAGTAGGCATGG+TGG 0.683415 5:+64441204 None:intergenic
TGTGTACAACAATTGCCGGA+GGG 0.684042 5:-64446700 MsaT028584.1:CDS
AGAAGGAGAACAAACCACAA+GGG 0.699103 5:-64433252 MsaT028584.1:CDS
CATGAAGGAGAAATCAGCCG+CGG 0.700965 5:+64446485 None:intergenic
GATTGGTCGAGATCTGACAA+AGG 0.707472 5:-64446220 MsaT028584.1:CDS
GGGATCGGCAAACTCCATGG+AGG 0.756962 5:+64446145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 64433060 64446946 64433060 ID=MsaG028584
Chr5 mRNA 64433060 64446946 64433060 ID=MsaT028584.1;Parent=MsaG028584
Chr5 exon 64433060 64433287 64433060 ID=MsaT028584.1.exon7;Parent=MsaT028584.1
Chr5 CDS 64433060 64433287 64433060 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64440008 64440387 64440008 ID=MsaT028584.1.exon6;Parent=MsaT028584.1
Chr5 CDS 64440008 64440387 64440008 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64441173 64441384 64441173 ID=MsaT028584.1.exon5;Parent=MsaT028584.1
Chr5 CDS 64441173 64441384 64441173 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64443985 64444125 64443985 ID=MsaT028584.1.exon4;Parent=MsaT028584.1
Chr5 CDS 64443985 64444125 64443985 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64445753 64445858 64445753 ID=MsaT028584.1.exon3;Parent=MsaT028584.1
Chr5 CDS 64445753 64445858 64445753 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64446025 64446314 64446025 ID=MsaT028584.1.exon2;Parent=MsaT028584.1
Chr5 CDS 64446025 64446314 64446025 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Chr5 exon 64446375 64446946 64446375 ID=MsaT028584.1.exon1;Parent=MsaT028584.1
Chr5 CDS 64446375 64446946 64446375 ID=cds.MsaT028584.1;Parent=MsaT028584.1
Gene Sequence

>MsaT028584.1

ATGATGGAACAACTCGGGACTTATGTGAAGGCGCTTGAGCACGAAAATTCAAATCTCATTCAAATTATTTCAGAGCATGTAAAAAAATACATTGAAGAACACTCTCAATTGGTGGTGATCGATCCAAACCTAATGATGGATGCGTTGAAGCCAGAAACCATTAACGACCTTGAGGAAATAGCAAAGGTCATGTGTATGGCTGGGTTTGAGAAGGATTTTTCCGATGTGTACAACAATTGCCGGAGGGAATGCTTGGACAAGTGCCTAATGCATAAATTATTCGGCTTACAGAAGCTCAGTATTGAGGACGTTCACAATATGTCATGGAAGGATCTTGAAGACAAGATTGAAAGATGGATTAAAACTTTCAATGTCACTCTCAAGATACTTTTCCCTGGCGAGCGAAGACTTTGTGACCGCATCTTCTTTGGATTCTCATCCGCGGCTGATTTCTCCTTCATGGAGATTTGCAAGGAATCCACCATTCAACTTCTAAACTTTGTTGATTATGTGTCCAGTGGAAGTCACTCACCAGAGCGTTTGTTTAAAATCCTTGAGGTTTTTGAAACATTGAATGAAGCAACTGCTATATGGAAGAGACTGGGGAAAACAATTAGGGACATATTTAAGGAGTTGGAGTATTTGATTGGTCGAGATCTGACAAAGGCGACAAATTTTGGTGGCGGTCTTCACCCAATTACCCAACATGTGATGAACTACCTCCATGGAGTTTGCCGATCCCAGCAAACATTAGAGCAAGTCTTTTACGATTCCTCACTTTCTTCAAAGATTCGTAGAATTATGGATACATTGGAGAGCAATTTGGAAGAAAAATCTATATGCTACGAGGACTCTTCTTTAGTGCTAGATTTTTTCCGGTCAAGAAATGATGGCGGGGAAGGATCAAAGGTGTATCAAGGAGATCATATGAAAGAAATAGAAGTATCACCCTCGCAACCAGGAGAAAGGAGAAAGAAATTGGAGACAGAGATAATAACAATAGTGATCTCTCCCGAACCCCAGTTTGGGAACACATACAAAATGCCACCGGAAGACGGGTTTATCTGGAAAGAATATGGAAGGAAAGAGATACCTGGCTTCAACCCGAGGAGTTACTACAGGTGCAACCACATGAAATTATATGCGTGTCGAGCCAGGAAGAAAGTACAACAACTTGGTGACCATCCAAACATATTTGAAGTTATATACAACGGTGGACATACCTGCCGCATGTCCTTAACAATACCATCAATGGATGTTACTCAAACCACCATGCCTACTTCACAACTTTATATTCGGGTGGATTTCATCGGGCATACTGGAAAATCCATAAACTCTATGCCAGAGGCCATGTTAGCTGCTCCGACGGATGGTGGGCATCATCCTATGATGGTGAAAATTTTGGCCTATCTTGTTTCTGCTATAAGATCACAGCAGATCAAAGTTGTTAATGGAGACGGAACATTTTCAATACAAACTGAGGGGACAATGGAACTTTTAGAGAGCATATTAGCATCAAAGTCCGAAGAATATGCATACCCTTCTTTGCGTCATTTTTTCATGATGAATAATTGGAAATATTTAGAAGTGACAAATAGAACAAGTGAAATGGATGCAATTTTTGGCAATGATTGGTATAAAAAAATCAGAGCAAAAGTCCAACAAAACATTGAACTCTATCAAAGAAACTCATGGGATAAGCTAATAGTATTCAAGAAGGAGAACAAACCACAAGGGCAGTGGAAAGAACAAGAGATTAAAAGGGGAAGAAGGCATCCCACGCAGGCACCTAAACAACCACCTAACATGCTAAAGAAAACAAACCACAACAGTAGGAACTATAGCATAACAGCACCACAGTCAAAAACTAACACAGTAGAAACACCAGAAATTTTAACCGACCCTAAGAATCCAAAACCCCAACCTTAG
Protein sequence

>MsaT028584.1

MMEQLGTYVKALEHENSNLIQIISEHVKKYIEEHSQLVVIDPNLMMDALKPETINDLEEIAKVMCMAGFEKDFSDVYNNCRRECLDKCLMHKLFGLQKLSIEDVHNMSWKDLEDKIERWIKTFNVTLKILFPGERRLCDRIFFGFSSAADFSFMEICKESTIQLLNFVDYVSSGSHSPERLFKILEVFETLNEATAIWKRLGKTIRDIFKELEYLIGRDLTKATNFGGGLHPITQHVMNYLHGVCRSQQTLEQVFYDSSLSSKIRRIMDTLESNLEEKSICYEDSSLVLDFFRSRNDGGEGSKVYQGDHMKEIEVSPSQPGERRKKLETEIITIVISPEPQFGNTYKMPPEDGFIWKEYGRKEIPGFNPRSYYRCNHMKLYACRARKKVQQLGDHPNIFEVIYNGGHTCRMSLTIPSMDVTQTTMPTSQLYIRVDFIGHTGKSINSMPEAMLAAPTDGGHHPMMVKILAYLVSAIRSQQIKVVNGDGTFSIQTEGTMELLESILASKSEEYAYPSLRHFFMMNNWKYLEVTNRTSEMDAIFGNDWYKKIRAKVQQNIELYQRNSWDKLIVFKKENKPQGQWKEQEIKRGRRHPTQAPKQPPNMLKKTNHNSRNYSITAPQSKTNTVETPEILTDPKNPKPQP*