Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028624 | XP_039683260.1 | 75.309 | 81 | 20 | 0 | 43 | 123 | 96 | 176 | 2.67e-35 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028624 | tr|A0A1S2Z7J1|A0A1S2Z7J1_CICAR | 70.930 | 86 | 25 | 0 | 38 | 123 | 84 | 169 | 1.46e-35 | 133 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG028624 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028624 | MtrunA17_Chr6g0451441 | 62.963 | 81 | 30 | 0 | 43 | 123 | 147 | 227 | 4.60e-31 | 115 |
| MsaG028624 | MtrunA17_Chr5g0441581 | 52.174 | 92 | 36 | 1 | 31 | 122 | 82 | 165 | 2.63e-22 | 91.3 |
| MsaG028624 | MtrunA17_Chr2g0296781 | 33.766 | 77 | 51 | 0 | 46 | 122 | 61 | 137 | 2.14e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCCTGCTTCATCATTGATT+TGG | 0.240616 | 5:-66042939 | None:intergenic |
| TGAAGACACTTCCGACTAAA+AGG | 0.299870 | 5:+66042761 | MsaT028624.1:intron |
| AACTTAGCAGTAATCTTTGC+AGG | 0.345757 | 5:-66042793 | None:intergenic |
| AATGGAGTTTATAGCTTTAG+TGG | 0.348005 | 5:+66042535 | MsaT028624.1:CDS |
| TCACAAACATCAAAATTGTT+TGG | 0.358937 | 5:-66042493 | None:intergenic |
| AGTTGGGAAAAGATGAATTA+TGG | 0.360559 | 5:+66042812 | MsaT028624.1:CDS |
| CTTGAATAACCTTGACTTAG+TGG | 0.398152 | 5:-66042878 | None:intergenic |
| GCAAGTTGCGAGCCTTCATC+AGG | 0.400046 | 5:+66042565 | MsaT028624.1:CDS |
| TGCAAAGATTACTGCTAAGT+TGG | 0.422853 | 5:+66042795 | MsaT028624.1:CDS |
| CTAATTGTGTGAGAGTGGTT+AGG | 0.441187 | 5:-66042853 | None:intergenic |
| CACCTCCTCTACAGATGAAA+AGG | 0.453866 | 5:-66042593 | None:intergenic |
| AATAGAGAAAGGCGTATCAT+TGG | 0.465390 | 5:+66043054 | MsaT028624.1:intron |
| TTGAATAACCTTGACTTAGT+GGG | 0.483800 | 5:-66042877 | None:intergenic |
| CACTAAGTCAAGGTTATTCA+AGG | 0.496285 | 5:+66042879 | MsaT028624.1:CDS |
| GCAAAGATTACTGCTAAGTT+GGG | 0.503061 | 5:+66042796 | MsaT028624.1:CDS |
| GAAAGGCGTATCATTGGAAA+AGG | 0.517095 | 5:+66043060 | MsaT028624.1:CDS |
| GAAAAGGACATCCCTGATGA+AGG | 0.537910 | 5:-66042577 | None:intergenic |
| ATCCAAATCAATGATGAAGC+AGG | 0.543887 | 5:+66042937 | MsaT028624.1:CDS |
| CAGACTATTGTTAGAGACGC+TGG | 0.544566 | 5:+66042982 | MsaT028624.1:CDS |
| TGGGGCTAATTGTGTGAGAG+TGG | 0.603064 | 5:-66042858 | None:intergenic |
| TGAATAACCTTGACTTAGTG+GGG | 0.603118 | 5:-66042876 | None:intergenic |
| AGGCGTATCATTGGAAAAGG+AGG | 0.630655 | 5:+66043063 | MsaT028624.1:CDS |
| TCAATGATGAAGCAGGCGTG+AGG | 0.657093 | 5:+66042944 | MsaT028624.1:CDS |
| CAATTAGCCCCACTAAGTCA+AGG | 0.658920 | 5:+66042869 | MsaT028624.1:CDS |
| CAAGTTGCGAGCCTTCATCA+GGG | 0.660640 | 5:+66042566 | MsaT028624.1:CDS |
| ACTATTGTTAGAGACGCTGG+AGG | 0.710750 | 5:+66042985 | MsaT028624.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 66042484 | 66043105 | 66042484 | ID=MsaG028624 |
| Chr5 | mRNA | 66042484 | 66043105 | 66042484 | ID=MsaT028624.1;Parent=MsaG028624 |
| Chr5 | exon | 66042484 | 66042612 | 66042484 | ID=MsaT028624.1.exon1;Parent=MsaT028624.1 |
| Chr5 | CDS | 66042484 | 66042612 | 66042484 | ID=cds.MsaT028624.1;Parent=MsaT028624.1 |
| Chr5 | exon | 66042766 | 66043006 | 66042766 | ID=MsaT028624.1.exon2;Parent=MsaT028624.1 |
| Chr5 | CDS | 66042766 | 66043006 | 66042766 | ID=cds.MsaT028624.1;Parent=MsaT028624.1 |
| Chr5 | exon | 66043059 | 66043105 | 66043059 | ID=MsaT028624.1.exon3;Parent=MsaT028624.1 |
| Chr5 | CDS | 66043059 | 66043105 | 66043059 | ID=cds.MsaT028624.1;Parent=MsaT028624.1 |
| Gene Sequence |
| Protein sequence |
