AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028669


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028669 MtrunA17_Chr5g0429931 85.356 239 26 1 36 265 10 248 6.35e-152 423
MsaG028669 MtrunA17_Chr3g0077451 69.295 241 54 3 35 265 10 240 1.25e-113 326
MsaG028669 MtrunA17_Chr3g0077411 64.045 267 79 3 16 265 257 523 9.06e-113 334
MsaG028669 MtrunA17_Chr3g0077411 54.135 266 86 5 1 253 1 243 1.25e-84 262
MsaG028669 MtrunA17_Chr3g0077431 67.299 211 49 3 65 265 1 201 1.39e-94 276
MsaG028669 MtrunA17_Chr3g0077421 64.762 210 53 3 65 263 1 200 4.55e-89 263
MsaG028669 MtrunA17_Chr3g0077441 64.762 210 53 3 65 263 1 200 4.55e-89 263
MsaG028669 MtrunA17_Chr3g0077361 54.682 267 88 4 1 256 20 264 8.46e-88 270
MsaG028669 MtrunA17_Chr3g0077361 52.555 274 84 3 3 265 269 507 3.17e-85 263
MsaG028669 MtrunA17_Chr4g0009491 58.667 225 83 3 3 217 206 430 2.20e-82 253
MsaG028669 MtrunA17_Chr4g0009491 50.704 213 74 2 35 238 11 201 2.54e-61 199
MsaG028669 MtrunA17_Chr3g0077381 58.716 218 87 2 3 217 228 445 8.02e-81 250
MsaG028669 MtrunA17_Chr3g0077381 48.069 233 66 4 35 256 34 222 7.52e-58 191
MsaG028669 MtrunA17_Chr3g0077461 54.000 250 93 8 16 257 232 467 8.34e-78 243
MsaG028669 MtrunA17_Chr3g0077461 49.789 237 79 4 36 262 22 228 1.07e-67 216
MsaG028669 MtrunA17_Chr1g0162441 48.485 99 51 0 21 119 7 105 7.90e-30 116
MsaG028669 MtrunA17_Chr3g0103171 28.244 262 148 5 36 258 5 265 2.68e-26 103
MsaG028669 MtrunA17_Chr4g0033961 36.025 161 97 2 18 173 3 162 3.69e-26 105
MsaG028669 MtrunA17_Chr3g0103181 28.364 275 155 6 20 258 11 279 7.23e-25 100
MsaG028669 MtrunA17_Chr7g0233521 27.985 268 148 7 37 262 2 266 1.15e-24 99.8
MsaG028669 MtrunA17_Chr3g0131891 26.644 289 166 6 20 262 18 306 1.25e-24 100
MsaG028669 MtrunA17_Chr1g0162411 48.889 90 46 0 21 110 7 96 2.80e-24 100
MsaG028669 MtrunA17_Chr7g0233561 27.070 314 169 8 7 262 4 315 5.57e-23 95.9
MsaG028669 MtrunA17_Chr8g0390841 23.569 297 165 6 21 259 39 331 7.33e-21 91.7
MsaG028669 MtrunA17_Chr7g0233531 28.253 269 146 8 37 262 2 266 3.33e-20 87.4
MsaG028669 MtrunA17_Chr5g0419201 29.697 165 105 2 21 185 16 169 3.55e-20 85.5
MsaG028669 MtrunA17_Chr1g0163071 24.913 289 148 6 37 259 2 287 6.41e-20 87.0
MsaG028669 MtrunA17_Chr7g0233571 33.571 140 81 1 13 140 2 141 6.55e-20 85.5
MsaG028669 MtrunA17_Chr7g0233541 25.965 285 149 7 37 262 2 283 1.67e-19 85.9
MsaG028669 MtrunA17_Chr1g0154161 23.404 282 169 6 20 258 29 306 8.74e-19 84.3
MsaG028669 MtrunA17_Chr1g0208771 24.199 281 156 8 36 260 12 291 9.77e-19 85.1
MsaG028669 MtrunA17_Chr3g0103151 40.000 100 58 1 20 119 11 108 6.66e-18 78.6
MsaG028669 MtrunA17_Chr1g0162421 50.000 58 29 0 35 92 7 64 7.56e-18 75.5
MsaG028669 MtrunA17_Chr3g0103191 29.469 207 113 4 21 199 12 213 9.45e-18 80.1
MsaG028669 MtrunA17_Chr1g0163221 25.685 292 169 9 12 258 2 290 5.78e-17 79.3
MsaG028669 MtrunA17_Chr5g0421921 39.623 106 54 2 167 263 8 112 4.32e-16 72.4
MsaG028669 MtrunA17_Chr5g0421731 39.623 106 54 2 167 263 8 112 4.32e-16 72.4
MsaG028669 MtrunA17_Chr5g0421851 39.623 106 54 2 167 263 83 187 4.23e-15 71.6
MsaG028669 MtrunA17_Chr6g0467351 31.655 139 91 2 20 154 14 152 7.49e-15 72.0
MsaG028669 MtrunA17_Chr4g0067431 24.917 301 161 8 20 258 14 311 1.72e-14 72.0
MsaG028669 MtrunA17_Chr3g0103201 25.339 221 118 6 80 258 5 220 6.88e-13 66.2
MsaG028669 MtrunA17_Chr1g0154041 30.928 97 66 1 20 116 15 110 1.68e-12 67.0
MsaG028669 MtrunA17_Chr1g0163061 30.769 130 80 2 20 139 14 143 3.67e-12 65.1
MsaG028669 MtrunA17_Chr1g0163251 21.691 272 170 8 30 261 14 282 3.76e-12 65.9
MsaG028669 MtrunA17_Chr1g0154101 31.579 95 65 0 20 114 24 118 1.14e-11 64.7
MsaG028669 MtrunA17_Chr1g0154101 34.737 95 60 2 20 114 183 275 2.41e-11 63.5
MsaG028669 MtrunA17_Chr1g0154191 33.684 95 63 0 20 114 21 115 4.56e-11 62.4
MsaG028669 MtrunA17_Chr1g0154171 36.709 79 50 0 33 111 34 112 5.68e-11 62.0
MsaG028669 MtrunA17_Chr1g0154071 33.684 95 62 1 20 114 27 120 7.87e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028669 AT3G18990.1 48.485 99 51 0 21 119 6 104 4.93e-26 104
MsaG028669 AT3G18990.2 48.485 99 51 0 21 119 6 104 7.93e-26 104
MsaG028669 AT4G01580.1 37.302 126 71 1 4 121 5 130 1.04e-19 84.7
MsaG028669 AT3G18960.2 38.835 103 63 0 19 121 28 130 3.64e-18 80.5
MsaG028669 AT3G18960.1 38.835 103 63 0 19 121 28 130 5.61e-18 80.5
MsaG028669 AT1G49475.1 37.037 135 75 2 4 129 8 141 1.30e-17 79.0
MsaG028669 AT3G18960.3 38.636 88 54 0 34 121 6 93 2.28e-13 67.0
MsaG028669 AT4G31690.1 25.000 236 156 7 19 248 12 232 2.22e-12 65.9
MsaG028669 AT4G31640.1 23.846 260 152 9 18 259 11 242 6.09e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCCCTCCATGAGAATTT+TGG 0.201758 5:+66822629 None:intergenic
AGTGAAGCTTACTATTTCTT+TGG 0.240401 5:+66824452 None:intergenic
GATGAATTTATATCAAGATT+TGG 0.269670 5:-66823213 MsaT028669.1:CDS
CAACGAAAATAAGGGTTGTT+TGG 0.270085 5:+66822811 None:intergenic
TACTATTTCTTTGGAATGAA+TGG 0.303913 5:+66824461 None:intergenic
AGATGGTCGTGTCTGGAAAA+TGG 0.306967 5:-66823154 MsaT028669.1:CDS
TTATAGGAACATTTGGCTTC+AGG 0.316899 5:+66822656 None:intergenic
ATGAATTTATATCAAGATTT+GGG 0.336114 5:-66823212 MsaT028669.1:CDS
CCTTCTCCGCCGCTGATGTC+TGG 0.353245 5:+66824520 None:intergenic
ATGAATGGGTGAGGGAAGTA+TGG 0.359095 5:+66824476 None:intergenic
TCCCTTCATATTTGAAAGAA+AGG 0.361097 5:+66823046 None:intergenic
ATTATCCTAGTTCTAGAAAA+AGG 0.363374 5:-66822930 MsaT028669.1:CDS
TGGAGCTTTATAGGAACATT+TGG 0.363518 5:+66822649 None:intergenic
AACGAAAATAAGGGTTGTTT+GGG 0.366185 5:+66822812 None:intergenic
GAATACTATTCTATAGGATA+TGG 0.373789 5:-66823075 MsaT028669.1:CDS
ATGTGAGAGGCGTGGGAAAA+AGG 0.385584 5:-66822893 MsaT028669.1:CDS
TTCCTTTCTTTCAAATATGA+AGG 0.389522 5:-66823048 MsaT028669.1:CDS
AATCTTGATATAAATTCATC+TGG 0.413169 5:+66823216 None:intergenic
CTGTTCCAGATGGTCGTGTC+TGG 0.416931 5:-66823161 MsaT028669.1:CDS
GTTGTTTGGGTTGAGTTCCT+TGG 0.432159 5:+66822825 None:intergenic
ACTATTTCTTTGGAATGAAT+GGG 0.437997 5:+66824462 None:intergenic
TTTGTAGAATACTATTCTAT+AGG 0.438154 5:-66823081 MsaT028669.1:CDS
GGAGGGCAGTGGGAAGTGTT+TGG 0.442028 5:-66822616 MsaT028669.1:CDS
TTCTTTGGAATGAATGGGTG+AGG 0.464859 5:+66824467 None:intergenic
CCTCTCACATGTTTCAACCT+TGG 0.481695 5:+66822906 None:intergenic
CCAGACATCAGCGGCGGAGA+AGG 0.489663 5:-66824520 MsaT028669.1:CDS
ATAAAGCTCCAAAATTCTCA+TGG 0.500218 5:-66822637 MsaT028669.1:CDS
AAAATTCTCATGGAGGGCAG+TGG 0.508854 5:-66822627 MsaT028669.1:CDS
TCCTTTCTTTCAAATATGAA+GGG 0.512330 5:-66823047 MsaT028669.1:CDS
TTGCAACAATGCAGCCTCGC+CGG 0.517685 5:-66824554 None:intergenic
TTCAAAACTTCAAGTACTAA+CGG 0.531313 5:-66822967 MsaT028669.1:CDS
TCTTTGGAATGAATGGGTGA+GGG 0.542125 5:+66824468 None:intergenic
TTGAAACATGTGAGAGGCGT+GGG 0.548958 5:-66822900 MsaT028669.1:CDS
TTTCGTTGCAAAATCGTCAA+GGG 0.551442 5:-66822796 MsaT028669.1:CDS
AGCTCCAAAATTCTCATGGA+GGG 0.568287 5:-66822633 MsaT028669.1:CDS
AAGAGTAGAGAATGCTGCCA+AGG 0.581714 5:-66822842 MsaT028669.1:CDS
AAATTCTCATGGAGGGCAGT+GGG 0.584828 5:-66822626 MsaT028669.1:CDS
ATCAGCAATGCAAATAGTGA+GGG 0.596191 5:-66822572 MsaT028669.1:CDS
GTTGAAACATGTGAGAGGCG+TGG 0.612877 5:-66822901 MsaT028669.1:CDS
GCTACAATTACTGTTCCAGA+TGG 0.621864 5:-66823171 MsaT028669.1:CDS
ATGTCTGGACACACTTTCGC+CGG 0.627588 5:+66824535 None:intergenic
CATCAGCAATGCAAATAGTG+AGG 0.629339 5:-66822573 MsaT028669.1:CDS
TGGACACACTTTCGCCGGCG+AGG 0.634315 5:+66824540 None:intergenic
AAGCTCCAAAATTCTCATGG+AGG 0.639241 5:-66822634 MsaT028669.1:CDS
GTGTGTTTGAGCTGATCAAG+AGG 0.642202 5:-66822501 MsaT028669.1:CDS
AGGGACAACAAACTGTCACA+TGG 0.650637 5:-66822532 MsaT028669.1:CDS
CCAAGGTTGAAACATGTGAG+AGG 0.654125 5:-66822906 MsaT028669.1:CDS
GTGTGTCCAGACATCAGCGG+CGG 0.681356 5:-66824526 MsaT028669.1:CDS
AATCGTCAAGGGAAAATATG+CGG 0.692781 5:-66822785 MsaT028669.1:intron
TCAGCAATGCAAATAGTGAG+GGG 0.699768 5:-66822571 MsaT028669.1:CDS
AAAGTGTGTCCAGACATCAG+CGG 0.761993 5:-66824529 MsaT028669.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 66822447 66824568 66822447 ID=MsaG028669
Chr5 mRNA 66822447 66824568 66822447 ID=MsaT028669.1;Parent=MsaG028669
Chr5 exon 66822447 66822680 66822447 ID=MsaT028669.1.exon3;Parent=MsaT028669.1
Chr5 CDS 66822447 66822680 66822447 ID=cds.MsaT028669.1;Parent=MsaT028669.1
Chr5 exon 66822786 66823244 66822786 ID=MsaT028669.1.exon2;Parent=MsaT028669.1
Chr5 CDS 66822786 66823244 66822786 ID=cds.MsaT028669.1;Parent=MsaT028669.1
Chr5 exon 66824464 66824568 66824464 ID=MsaT028669.1.exon1;Parent=MsaT028669.1
Chr5 CDS 66824464 66824568 66824464 ID=cds.MsaT028669.1;Parent=MsaT028669.1
Gene Sequence

>MsaT028669.1

ATGCAGCCTCGCCGGCGAAAGTGTGTCCAGACATCAGCGGCGGAGAAGGAATCAAAACATTTTATGAAAGCCATACTTCCCTCACCCATTCATTCCAAAGAAATAAGAATTCCAGATGAATTTATATCAAGATTTGGGAATGAACTCAAGAATGTTGCTACAATTACTGTTCCAGATGGTCGTGTCTGGAAAATGGAACTGAAGAAACATGATGAGAACGTTTTCTTTTGCAACAAATGGCAAGAGTTTGTAGAATACTATTCTATAGGATATGGTTGCTTCCTTTCTTTCAAATATGAAGGGAATTCAAAGTTTAGTGCTATTATATTTGATGCAACTTCTGTTGAAATTTGCTATCCTTTCAAAACTTCAAGTACTAACGGAGAACCAAAAACAAATTATCCTAGTTCTAGAAAAAGGTCCAAGGTTGAAACATGTGAGAGGCGTGGGAAAAAGGTCAAAAACATGTCTAAATATGCTTCTAAAAGAGTAGAGAATGCTGCCAAGGAACTCAACCCAAACAACCCTTATTTTCGTTGCAAAATCGTCAAGGGAAAATATGCGTACCTGAAGCCAAATGTTCCTATAAAGCTCCAAAATTCTCATGGAGGGCAGTGGGAAGTGTTTGGCATTATTCATGATGTCAGATCATCAGCAATGCAAATAGTGAGGGGATTTTCTATATTTCAAAGGGACAACAAACTGTCACATGGAGATTATTGTGTGTTTGAGCTGATCAAGAGGAGACCGATTGTGCTAAAAGTCACAATGTTTCGTGCAGTTGACTATTGCGATTAA

Protein sequence

>MsaT028669.1

MQPRRRKCVQTSAAEKESKHFMKAILPSPIHSKEIRIPDEFISRFGNELKNVATITVPDGRVWKMELKKHDENVFFCNKWQEFVEYYSIGYGCFLSFKYEGNSKFSAIIFDATSVEICYPFKTSSTNGEPKTNYPSSRKRSKVETCERRGKKVKNMSKYASKRVENAAKELNPNNPYFRCKIVKGKYAYLKPNVPIKLQNSHGGQWEVFGIIHDVRSSAMQIVRGFSIFQRDNKLSHGDYCVFELIKRRPIVLKVTMFRAVDYCD*