AlfalfaGEDB | MsaG028689

M. sativa ssp. caerulea / MsaG028689

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF12796.10

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028689	XP_003615688.2	95.332	707	27	3	1	704	1	704	0.0	1354

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028689	sp\|Q9LUZ4\|C3H66_ARATH	47.254	692	267	19	23	703	2	606	0.0	572

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028689	tr\|G7JZH7\|G7JZH7_MEDTR	95.332	707	27	3	1	704	1	704	0.0	1354

TFs/TRs:

Gene ID	Type	Classification
MsaG028689	TF	C3H

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001878	MsaG028689	0.827636	2.504095e-54	6.778253e-52
MsaG002720	MsaG028689	0.816917	7.506751e-52	1.523757e-49
MsaG004153	MsaG028689	0.823350	2.564615e-53	6.168282e-51
MsaG004872	MsaG028689	0.801324	1.591751e-48	2.217490e-46
MsaG004930	MsaG028689	0.806856	1.139056e-49	1.803741e-47
MsaG006407	MsaG028689	0.809098	3.816835e-50	6.377964e-48
MsaG006480	MsaG028689	0.811770	1.017723e-50	1.814827e-48
MsaG006757	MsaG028689	0.848045	1.488971e-59	7.552339e-57
MsaG007092	MsaG028689	0.819039	2.500868e-52	5.362974e-50
MsaG007551	MsaG028689	0.824264	1.570141e-53	3.871099e-51
MsaG007737	MsaG028689	0.866959	3.915729e-65	4.004082e-62
MsaG008223	MsaG028689	0.812020	8.984538e-51	1.612016e-48
MsaG008615	MsaG028689	0.804564	3.431409e-49	5.149933e-47
MsaG012060	MsaG028689	0.834659	4.800173e-56	1.591646e-53
MsaG014205	MsaG028689	0.840455	1.592403e-57	6.305609e-55
MsaG015721	MsaG028689	0.851381	1.759944e-60	1.000358e-57
MsaG016247	MsaG028689	0.804781	3.094395e-49	4.667425e-47
MsaG017341	MsaG028689	0.805515	2.175522e-49	3.337831e-47
MsaG018881	MsaG028689	0.808867	4.275278e-50	7.104362e-48
MsaG020336	MsaG028689	0.809816	2.680763e-50	4.557842e-48
MsaG020815	MsaG028689	0.823694	2.132894e-53	5.178055e-51
MsaG020838	MsaG028689	0.819210	2.287922e-52	4.928361e-50
MsaG023894	MsaG028689	0.832688	1.483291e-55	4.639706e-53
MsaG027138	MsaG028689	0.838476	5.170594e-57	1.924570e-54
MsaG027514	MsaG028689	0.802179	1.064786e-48	1.512446e-46
MsaG027591	MsaG028689	0.810873	1.589555e-50	2.772892e-48
MsaG027763	MsaG028689	0.829322	9.855417e-55	2.797204e-52
MsaG028119	MsaG028689	0.844082	1.760943e-58	7.829287e-56
MsaG028135	MsaG028689	0.814303	2.850195e-51	5.413890e-49
MsaG028689	MsaG029235	0.866753	4.550882e-65	4.614201e-62
MsaG028689	MsaG029381	0.806885	1.123109e-49	1.779716e-47
MsaG028689	MsaG029430	0.818221	3.826583e-52	8.032817e-50
MsaG028689	MsaG034426	0.812252	7.999734e-51	1.443526e-48
MsaG028689	MsaG035363	0.802078	1.117041e-48	1.583027e-46
MsaG028689	MsaG035414	0.805964	1.752812e-49	2.717800e-47
MsaG028689	MsaG035660	0.801900	1.214434e-48	1.714145e-46
MsaG028689	MsaG035872	0.813362	4.582126e-51	8.500687e-49
MsaG028689	MsaG037420	0.804325	3.847405e-49	5.742418e-47
MsaG028689	MsaG038007	0.826462	4.767235e-54	1.248844e-51
MsaG028689	MsaG038422	0.806758	1.194570e-49	1.887358e-47
MsaG028689	MsaG044728	0.850515	3.079209e-60	1.698661e-57
MsaG028689	MsaG046538	0.817564	5.377774e-52	1.109913e-49
MsaG028689	MsaG046977	0.805386	2.314866e-49	3.541062e-47
MsaG028689	MsaG001346	0.826042	5.992801e-54	1.551659e-51
MsaG028689	MsaG014896	0.808610	4.847653e-50	8.005553e-48
MsaG028689	MsaG013522	0.815330	1.691563e-51	3.297356e-49
MsaG028689	MsaG031242	0.837019	1.219067e-56	4.338769e-54
MsaG028689	MsaG030699	0.837236	1.073518e-56	3.846052e-54
MsaG028689	MsaG033422	0.813544	4.180579e-51	7.791232e-49
MsaG028689	MsaG036646	0.848689	9.900394e-60	5.131180e-57
MsaG028689	MsaG036687	0.810131	2.294964e-50	3.931628e-48
MsaG028689	MsaG046905	0.816008	1.196363e-51	2.372484e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028689	MtrunA17_Chr5g0430211	95.332	707	27	3	1	704	1	704	0.0	1354
MsaG028689	MtrunA17_Chr5g0437251	49.928	693	293	14	30	703	25	682	0.0	634
MsaG028689	MtrunA17_Chr1g0187591	53.917	651	207	14	1	640	1	569	0.0	612
MsaG028689	MtrunA17_Chr3g0105131	45.763	649	195	18	1	639	1	502	3.56e-155	459
MsaG028689	MtrunA17_Chr7g0257831	40.847	732	316	23	35	699	47	728	4.45e-155	466
MsaG028689	MtrunA17_Chr3g0088521	40.055	729	332	20	33	699	43	728	1.54e-146	444
MsaG028689	MtrunA17_Chr3g0105101	46.465	495	140	12	39	530	29	401	3.24e-125	378
MsaG028689	MtrunA17_Chr3g0113651	45.662	438	168	5	33	469	8	376	5.12e-124	379
MsaG028689	MtrunA17_Chr3g0113651	32.719	217	119	9	483	695	322	515	3.27e-18	88.6
MsaG028689	MtrunA17_Chr1g0171151	39.926	541	257	15	35	537	31	541	1.53e-107	339
MsaG028689	MtrunA17_Chr3g0092171	44.142	367	159	8	32	378	80	420	2.93e-89	288
MsaG028689	MtrunA17_Chr3g0092221	40.896	335	140	10	138	451	51	348	1.70e-72	241
MsaG028689	MtrunA17_Chr3g0105121	37.273	440	175	14	229	658	8	356	9.76e-67	224
MsaG028689	MtrunA17_Chr3g0105141	65.000	140	47	2	199	336	4	143	1.06e-61	204
MsaG028689	MtrunA17_Chr8g0389561	43.498	223	115	3	162	381	21	235	1.09e-54	192
MsaG028689	MtrunA17_Chr6g0487481	57.971	138	48	2	196	332	77	205	4.42e-52	185
MsaG028689	MtrunA17_Chr3g0135351	49.419	172	73	3	202	372	32	190	9.33e-52	181
MsaG028689	MtrunA17_Chr2g0324781	61.261	111	43	0	230	340	77	187	2.15e-47	173
MsaG028689	MtrunA17_Chr4g0040391	57.009	107	46	0	226	332	56	162	2.85e-44	161
MsaG028689	MtrunA17_Chr3g0105151	33.904	292	112	13	394	669	1	227	4.42e-27	110
MsaG028689	MtrunA17_Chr3g0105111	40.870	115	42	4	594	703	17	110	1.18e-13	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028689	AT5G58620.1	47.254	692	267	19	23	703	2	606	0.0	572
MsaG028689	AT2G40140.2	42.416	712	284	17	1	703	1	595	4.16e-173	508
MsaG028689	AT2G40140.1	42.416	712	284	17	1	703	1	595	4.16e-173	508
MsaG028689	AT2G41900.1	38.599	728	319	20	35	699	48	710	6.17e-144	437
MsaG028689	AT3G55980.1	50.000	474	194	13	1	462	1	443	2.17e-142	429
MsaG028689	AT3G55980.1	37.313	201	87	8	508	703	413	579	1.43e-20	96.7
MsaG028689	AT3G55980.2	50.000	474	194	13	1	462	68	510	1.17e-141	429
MsaG028689	AT3G55980.2	37.313	201	87	8	508	703	480	646	2.01e-20	96.7
MsaG028689	AT5G12850.1	38.753	738	316	26	30	699	37	706	2.00e-140	428
MsaG028689	AT4G29190.1	52.273	176	83	1	212	387	62	236	1.61e-54	191
MsaG028689	AT2G25900.2	66.964	112	37	0	226	337	86	197	3.32e-53	186
MsaG028689	AT2G25900.1	66.964	112	37	0	226	337	86	197	3.32e-53	186
MsaG028689	AT2G19810.1	63.333	120	44	0	215	334	63	182	7.98e-53	186
MsaG028689	AT5G07500.1	52.703	148	66	1	189	332	7	154	1.16e-49	174
MsaG028689	AT1G03790.1	51.634	153	66	3	197	345	53	201	2.54e-47	172
MsaG028689	AT5G44260.1	55.970	134	54	2	210	342	44	173	2.62e-45	166

Find 187 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCAGTCTTCAATCTGCTTT+TGG	0.183105	5:-67137298	None:intergenic
TCAGACATGTCTTCTGTTTC+CGG	0.194365	5:+67136618	MsaT028689.1:CDS
GATGTATCGGCTTATATTCT+TGG	0.209122	5:+67136346	MsaT028689.1:CDS
CTCTTCATTGTGCGGTTGTT+GGG	0.225881	5:+67136419	MsaT028689.1:CDS
GGGCTCATCCGATGGAAATT+TGG	0.237284	5:+67137875	MsaT028689.1:CDS
AAAGTTGGTTCGAACAAATT+AGG	0.237750	5:+67136274	MsaT028689.1:CDS
CTGGCTGCAGCTGGAGTTAA+AGG	0.256754	5:-67137972	None:intergenic
GAGGATGCTACAAGCTCTTT+TGG	0.287126	5:+67136576	MsaT028689.1:CDS
TCCTGTGCCCTGGCTGCAGC+TGG	0.314964	5:-67137981	None:intergenic
ATACGACCGGGGAACTAAAT+AGG	0.321029	5:+67137484	MsaT028689.1:CDS
GCGTCCCTTGTCCTGAGTTT+CGG	0.326990	5:+67136890	MsaT028689.1:CDS
CTGAGGAAATCATCTTCATT+TGG	0.338854	5:+67137930	MsaT028689.1:CDS
GCTCTTCATTGTGCGGTTGT+TGG	0.359015	5:+67136418	MsaT028689.1:CDS
ACCTATAGGTTTGATCCATC+TGG	0.362218	5:+67137717	MsaT028689.1:CDS
GATTCATATTCGGACGCATT+TGG	0.363999	5:-67137641	None:intergenic
TGCAGATGCTGATTCATATT+CGG	0.365840	5:-67137651	None:intergenic
ACGCCGCCTTTGACTTCATC+TGG	0.368423	5:+67137201	MsaT028689.1:CDS
GTCTTTCCTCTCCTTCCAAC+TGG	0.371979	5:+67137412	MsaT028689.1:CDS
TATATAGTACCGATGAGTTT+AGG	0.375544	5:+67136746	MsaT028689.1:CDS
CGATTGGATCTCCGACCTAT+AGG	0.385130	5:+67137703	MsaT028689.1:CDS
CCTTGGATTGAAGATAGAAT+TGG	0.386827	5:-67136551	None:intergenic
CGCCCCTTGTATGCTTCTAC+CGG	0.390009	5:+67137066	MsaT028689.1:CDS
GATGGGGTTGGTTTGTGGTA+TGG	0.391400	5:+67136247	MsaT028689.1:CDS
AGCAAAATCTTGATAATCTC+GGG	0.396619	5:-67136457	None:intergenic
CAGCATCTGCAACAAAACTA+TGG	0.398990	5:+67137663	MsaT028689.1:CDS
CACATGGCTGCAGCAGCAAC+GGG	0.402327	5:-67137234	None:intergenic
ATTCAGATATTGCAATGCTT+AGG	0.408619	5:+67137340	MsaT028689.1:CDS
AAAATGTCTTCAAGGTTTGT+AGG	0.409953	5:-67137522	None:intergenic
TGGAATTTATGCAACATTGA+TGG	0.416189	5:-67137558	None:intergenic
TTGGGTTAATTCACTTGTAA+AGG	0.417539	5:+67138028	MsaT028689.1:CDS
TTCAGATATTGCAATGCTTA+GGG	0.419562	5:+67137341	MsaT028689.1:CDS
TCGGCTTATATTCTTGGAAC+AGG	0.420142	5:+67136352	MsaT028689.1:CDS
TTGTCAAAAGGATTTGATCA+TGG	0.420181	5:+67136099	MsaT028689.1:CDS
TAACGGAACAGATTCGATTC+CGG	0.421528	5:+67138118	MsaT028689.1:CDS
CTTGATGTGGCAGGAGCAGC+AGG	0.426394	5:+67137591	MsaT028689.1:CDS
TATATCCCATTCTTAATGTC+TGG	0.426530	5:-67136727	None:intergenic
CTCATGGTTGCAGCTATGTA+TGG	0.426552	5:+67136316	MsaT028689.1:CDS
GCTTGTAGCATCCTCTTCCT+TGG	0.429452	5:-67136568	None:intergenic
AAGCAAAATCTTGATAATCT+CGG	0.433411	5:-67136458	None:intergenic
GAGACCCGAAAATGTCTTCA+AGG	0.438096	5:-67137530	None:intergenic
AGATCCAATCGCACTGGAAG+TGG	0.440483	5:-67137691	None:intergenic
AAACTGCAGCGCCATCTTAA+CGG	0.443280	5:+67138101	MsaT028689.1:CDS
CTTATATTCTTGGAACAGGT+CGG	0.443594	5:+67136356	MsaT028689.1:CDS
ATGTTGATGGGGTTGGTTTG+TGG	0.450945	5:+67136242	MsaT028689.1:CDS
GAGTGTCCCTTTGTTCATCC+AGG	0.455762	5:+67136823	MsaT028689.1:CDS
GGTTGCGATGTTGATGGGGT+TGG	0.455769	5:+67136235	MsaT028689.1:CDS
TCAAAGTGAAGCCTTGTTCA+AGG	0.457380	5:+67136779	MsaT028689.1:CDS
GCAGTACTGTAATATGATCT+AGG	0.457857	5:-67137105	None:intergenic
AGCGAAACAATCAAATCAAC+TGG	0.459199	5:-67136526	None:intergenic
AGTTGATTTGATTGTTTCGC+TGG	0.460352	5:+67136528	MsaT028689.1:CDS
TCGAATCTGTTCCGTTAAGA+TGG	0.461972	5:-67138112	None:intergenic
CCACATGGCTGCAGCAGCAA+CGG	0.462166	5:-67137235	None:intergenic
TGTCAAAAGGATTTGATCAT+GGG	0.468107	5:+67136100	MsaT028689.1:CDS
CTTTAACTCCAGCTGCAGCC+AGG	0.469319	5:+67137973	MsaT028689.1:CDS
CTTCTTTGCTCACAAGGTCG+AGG	0.469734	5:+67137038	MsaT028689.1:CDS
ACGAGGGTTGCGATGTTGAT+GGG	0.471244	5:+67136230	MsaT028689.1:CDS
CATCCGATGGAAATTTGGAT+TGG	0.471302	5:+67137880	MsaT028689.1:CDS
TCTCTTCCAGACATTAAGAA+TGG	0.478120	5:+67136721	MsaT028689.1:CDS
GGAGCAATTGTACATGGATC+AGG	0.480663	5:+67138148	MsaT028689.1:CDS
CTCTTCCAGACATTAAGAAT+GGG	0.482849	5:+67136722	MsaT028689.1:CDS
TACTGCTTCTACATTAGAGA+TGG	0.486960	5:+67137122	MsaT028689.1:CDS
TATGTGAATGGGAATAAAGA+AGG	0.487426	5:+67136130	MsaT028689.1:CDS
CGGAAAGGATCGTGCAGTAA+AGG	0.487429	5:+67136910	MsaT028689.1:CDS
AGATTCGATTCCGGCTTGGT+TGG	0.488203	5:+67138127	MsaT028689.1:CDS
AGCTGGAGTTAAAGGAGTAC+TGG	0.489995	5:-67137964	None:intergenic
TTATATTCTTGGAACAGGTC+GGG	0.491005	5:+67136357	MsaT028689.1:CDS
TCTTCATTGTGCGGTTGTTG+GGG	0.492583	5:+67136420	MsaT028689.1:CDS
TTGTGGTATGGAAGGAAAGT+TGG	0.493676	5:+67136259	MsaT028689.1:CDS
ATGGGGTGTGAGTATGTGAA+TGG	0.494445	5:+67136118	MsaT028689.1:CDS
CAGGTCGGGTTGATGTTAAT+CGG	0.495738	5:+67136371	MsaT028689.1:CDS
CATTCCACTTCCAGTGCGAT+TGG	0.498841	5:+67137687	MsaT028689.1:CDS
GTATGGAAGCTTGGATGTAT+CGG	0.499389	5:+67136333	MsaT028689.1:CDS
TCTGGAAGAGAGACATCAAC+AGG	0.501027	5:-67136709	None:intergenic
TATTTAGTTCCCCGGTCGTA+TGG	0.502935	5:-67137482	None:intergenic
CCACAGAATTTCACTTGATG+TGG	0.503265	5:+67137578	MsaT028689.1:CDS
AGGAGATGGAATAGCAGAAC+CGG	0.503649	5:-67137085	None:intergenic
CCTATAGGTTTGATCCATCT+GGG	0.506082	5:+67137718	MsaT028689.1:CDS
ATGGAGAACCAAGTGACATT+GGG	0.506623	5:-67137155	None:intergenic
CTATGAACCAGATGTGTCTT+GGG	0.508226	5:+67138010	MsaT028689.1:CDS
CAAGGGCATATTCTCATGAC+TGG	0.511439	5:+67136797	MsaT028689.1:CDS
ACACGGCTTTGCAAGGATGA+AGG	0.512844	5:+67136994	MsaT028689.1:CDS
TGCAGCTATGTATGGAAGCT+TGG	0.514902	5:+67136324	MsaT028689.1:CDS
TAGTTCCCCGGTCGTATGGT+TGG	0.517395	5:-67137478	None:intergenic
GAATTAACCCAAGACACATC+TGG	0.517792	5:-67138017	None:intergenic
AGAATTTCACTTGATGTGGC+AGG	0.518433	5:+67137582	MsaT028689.1:CDS
CACCGCGCTCGATAAAGCTC+TGG	0.521693	5:-67137794	None:intergenic
GATGGAGAACCAAGTGACAT+TGG	0.523747	5:-67137156	None:intergenic
AACGAGGGTTGCGATGTTGA+TGG	0.524302	5:+67136229	MsaT028689.1:CDS
CGAAAACCTATTTAGTTCCC+CGG	0.524607	5:-67137490	None:intergenic
AGCCGTGTCTTATACTGAGC+AGG	0.529316	5:-67136979	None:intergenic
AGGGGAAAATGCAAGGCGAC+GGG	0.532175	5:+67136843	MsaT028689.1:CDS
GAACAGATTCGATTCCGGCT+TGG	0.533268	5:+67138123	MsaT028689.1:CDS
TCTAACTGGGGCTCATCCGA+TGG	0.533531	5:+67137867	MsaT028689.1:CDS
TCAATTAGCCCAATGTCACT+TGG	0.534086	5:+67137147	MsaT028689.1:CDS
TCCAGCTGCAGCCAGGGCAC+AGG	0.537339	5:+67137980	MsaT028689.1:CDS
TTGGTTGGAGCAATTGTACA+TGG	0.540592	5:+67138142	MsaT028689.1:CDS
TCTTCGACCAACCATACGAC+CGG	0.541735	5:+67137471	MsaT028689.1:CDS
TTTAACTCCAGCTGCAGCCA+GGG	0.542454	5:+67137974	MsaT028689.1:CDS
ACTATGAACCAGATGTGTCT+TGG	0.542920	5:+67138009	MsaT028689.1:CDS
ACTGCTTCTACATTAGAGAT+GGG	0.544371	5:+67137123	MsaT028689.1:CDS
TACAATTGCTCCAACCAAGC+CGG	0.546247	5:-67138137	None:intergenic
GTCAAAGGCGGCGTTGAGGA+GGG	0.547086	5:-67137192	None:intergenic
CTATCTTCAATCCAAGGAAG+AGG	0.548194	5:+67136557	MsaT028689.1:CDS
AGTTTGCTTCTTTGCTCACA+AGG	0.549883	5:+67137032	MsaT028689.1:CDS
CACGATCCTTTCCGAAACTC+AGG	0.550059	5:-67136901	None:intergenic
CAAAGTGAAGCCTTGTTCAA+GGG	0.551738	5:+67136780	MsaT028689.1:CDS
GCGAAAGCAGCAACTACTGA+TGG	0.552696	5:+67137377	MsaT028689.1:CDS
CCCAGATGGATCAAACCTAT+AGG	0.553523	5:-67137718	None:intergenic
CTTCGACCAACCATACGACC+GGG	0.555663	5:+67137472	MsaT028689.1:CDS
CCAATTCTATCTTCAATCCA+AGG	0.560551	5:+67136551	MsaT028689.1:CDS
CTGTAATATGATCTAGGAGA+TGG	0.561399	5:-67137099	None:intergenic
AGTCAAAGGCGGCGTTGAGG+AGG	0.561805	5:-67137193	None:intergenic
TCAATGTCATCAATTGTCTC+CGG	0.563566	5:-67136637	None:intergenic
TTCATCCAGGGGAAAATGCA+AGG	0.563820	5:+67136836	MsaT028689.1:CDS
AGAACCGGTAGAAGCATACA+AGG	0.565045	5:-67137070	None:intergenic
CCCAAAAGCAGATTGAAGAC+TGG	0.566689	5:+67137297	MsaT028689.1:CDS
CAGGGGAAAATGCAAGGCGA+CGG	0.567408	5:+67136842	MsaT028689.1:CDS
GGGTTGGTTTGTGGTATGGA+AGG	0.567780	5:+67136251	MsaT028689.1:CDS
GTATAAGACACGGCTTTGCA+AGG	0.567802	5:+67136987	MsaT028689.1:CDS
GTTGATGTTAATCGGTCTAG+TGG	0.567818	5:+67136379	MsaT028689.1:CDS
GGTCGGAGATCCAATCGCAC+TGG	0.570216	5:-67137697	None:intergenic
TCAGCTGCAGATGATGTGGT+TGG	0.573637	5:+67136187	MsaT028689.1:CDS
GAGGGTGGTATCAAAACAGA+TGG	0.574942	5:-67137174	None:intergenic
TGAAGAGAGGACACCTCTCA+TGG	0.576333	5:+67136300	MsaT028689.1:CDS
ACAAATTAGGTTATGAAGAG+AGG	0.576520	5:+67136287	MsaT028689.1:CDS
AGTGTCCCTTTGTTCATCCA+GGG	0.576667	5:+67136824	MsaT028689.1:CDS
CAATCGCACTGGAAGTGGAA+TGG	0.578710	5:-67137686	None:intergenic
ATGGAATGATTCCAGTTGGA+AGG	0.579291	5:-67137423	None:intergenic
CCTTTCCGAAACTCAGGACA+AGG	0.580350	5:-67136895	None:intergenic
ATGATTCCAGTTGGAAGGAG+AGG	0.583775	5:-67137418	None:intergenic
ATGAAGAGCAGTAGCACCAT+CGG	0.586647	5:-67136404	None:intergenic
ATGTGAATGGGAATAAAGAA+GGG	0.588204	5:+67136131	MsaT028689.1:CDS
GACGCGGATAACTCCCCAGA+TGG	0.588308	5:-67137732	None:intergenic
AGGCATGGAATGATTCCAGT+TGG	0.590112	5:-67137427	None:intergenic
CCTTGTCCTGAGTTTCGGAA+AGG	0.592498	5:+67136895	MsaT028689.1:CDS
CCGTTGCTGCTGCAGCCATG+TGG	0.592506	5:+67137235	MsaT028689.1:CDS
TGAGAATATGCCCTTGAACA+AGG	0.592999	5:-67136790	None:intergenic
ATCAACTGGTCGGTTACCGT+TGG	0.595630	5:-67136512	None:intergenic
ATCTGAATTAATATCTCTAG+CGG	0.595917	5:-67137325	None:intergenic
TGGGGTGTGAGTATGTGAAT+GGG	0.600624	5:+67136119	MsaT028689.1:CDS
CCACATCAAGTGAAATTCTG+TGG	0.602036	5:-67137578	None:intergenic
ATGACATTGACGAATATCAA+AGG	0.602422	5:+67136650	MsaT028689.1:CDS
ATCTGGGGAGTTATCCGCGT+CGG	0.603525	5:+67137734	MsaT028689.1:CDS
AATTTGGATTGGACTTCACA+CGG	0.603583	5:+67137891	MsaT028689.1:CDS
TGCTACTGCTCTTCATTGTG+CGG	0.605703	5:+67136411	MsaT028689.1:CDS
AAAAGATGGACTGTTAGGCA+TGG	0.606790	5:-67137442	None:intergenic
GTTGGAAGGAGAGGAAAGAC+CGG	0.607081	5:-67137409	None:intergenic
GGACGCATTTGGATTCCAGT+CGG	0.608359	5:-67137630	None:intergenic
GCAACTACTGATGGATGAGA+TGG	0.611701	5:+67137386	MsaT028689.1:CDS
CTACTGATGGATGAGATGGC+CGG	0.611784	5:+67137390	MsaT028689.1:CDS
GAGAATGAAGCTCAGAATGA+CGG	0.613447	5:-67137824	None:intergenic
AGCCAGAGCTTTATCGAGCG+CGG	0.613507	5:+67137792	MsaT028689.1:CDS
TGGATGAACAAAGGGACACT+CGG	0.617153	5:-67136821	None:intergenic
AATCGGTCTAGTGGATCCGA+TGG	0.617627	5:+67136388	MsaT028689.1:CDS
TGTATACATCCTAAACTCAT+CGG	0.621538	5:-67136755	None:intergenic
TACATAGCTGCAACCATGAG+AGG	0.624576	5:-67136313	None:intergenic
GCAAAATCTTGATAATCTCG+GGG	0.625212	5:-67136456	None:intergenic
TCTCAGTTACAATCTCCGAC+TGG	0.629713	5:+67137615	MsaT028689.1:CDS
GTCAAAAGGATTTGATCATG+GGG	0.630254	5:+67136101	MsaT028689.1:CDS
GTGTCCCTTTGTTCATCCAG+GGG	0.633464	5:+67136825	MsaT028689.1:CDS
CGAGGGTTGCGATGTTGATG+GGG	0.634282	5:+67136231	MsaT028689.1:CDS
CTTCATTGTGCGGTTGTTGG+GGG	0.635961	5:+67136421	MsaT028689.1:CDS
GATGCTCCAGATGAAGTCAA+AGG	0.639371	5:-67137207	None:intergenic
GATGGATCAAACCTATAGGT+CGG	0.639738	5:-67137714	None:intergenic
AAACAATCAAATCAACTGGT+CGG	0.641933	5:-67136522	None:intergenic
CTAAACTGATGAGATTCTTG+CGG	0.647291	5:-67138059	None:intergenic
AAAGGCGGCGTTGAGGAGGG+TGG	0.647882	5:-67137189	None:intergenic
AGTCCAATCCAAATTTCCAT+CGG	0.651783	5:-67137883	None:intergenic
GCTAGTGCTGTTGATGCCAA+CGG	0.653244	5:+67136496	MsaT028689.1:CDS
ATTAGACGGTGTCTGCCACA+TGG	0.653248	5:-67137250	None:intergenic
TAAAGAAGGGCAGTATCATG+AGG	0.655064	5:+67136144	MsaT028689.1:CDS
TGGAGTTAAAGGAGTACTGG+TGG	0.655843	5:-67137961	None:intergenic
TGAAGTCAAAGGCGGCGTTG+AGG	0.657176	5:-67137196	None:intergenic
GAGAAGAACTGAATAAGCTG+AGG	0.666361	5:+67137913	MsaT028689.1:CDS
TTCGACCAACCATACGACCG+GGG	0.672912	5:+67137473	MsaT028689.1:CDS
TGCTTAGGGTTGAAACTCAG+CGG	0.673294	5:+67137355	MsaT028689.1:CDS
AACCGGTAGAAGCATACAAG+GGG	0.673738	5:-67137068	None:intergenic
GAACCGGTAGAAGCATACAA+GGG	0.683674	5:-67137069	None:intergenic
CTATAGGTTTGATCCATCTG+GGG	0.685733	5:+67137719	MsaT028689.1:CDS
ATCCTGCTCAGTATAAGACA+CGG	0.689588	5:+67136977	MsaT028689.1:CDS
TTAGCGTTGAAGATCAGACA+AGG	0.695815	5:+67138078	MsaT028689.1:CDS
CAAAATCTTGATAATCTCGG+GGG	0.705772	5:-67136455	None:intergenic
TCTTGCATTCAAAGCCGACG+CGG	0.707580	5:-67137748	None:intergenic
GATTCTTGTGACTATGCACA+CGG	0.716065	5:+67136934	MsaT028689.1:CDS
CTTTCCGAAACTCAGGACAA+GGG	0.724035	5:-67136894	None:intergenic
GTAGGGATCGAAACATTAGA+CGG	0.728881	5:-67137264	None:intergenic
GCTCCAGATGAAGTCAAAGG+CGG	0.734283	5:-67137204	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	67136073	67138187	67136073	ID=MsaG028689
Chr5	mRNA	67136073	67138187	67136073	ID=MsaT028689.1;Parent=MsaG028689
Chr5	exon	67136073	67138187	67136073	ID=MsaT028689.1.exon1;Parent=MsaT028689.1
Chr5	CDS	67136073	67138187	67136073	ID=cds.MsaT028689.1;Parent=MsaT028689.1

Gene Sequence

>MsaT028689.1

ATGTGCAGTGATTCTAAGAAAAAAACTTGTCAAAAGGATTTGATCATGGGGTGTGAGTATGTGAATGGGAATAAAGAAGGGCAGTATCATGAGGTTTCTTATTTGCTTGAATTTTCAGCTGCAGATGATGTGGTTGGTTTAAAAAATGCGATTGAAAACGAGGGTTGCGATGTTGATGGGGTTGGTTTGTGGTATGGAAGGAAAGTTGGTTCGAACAAATTAGGTTATGAAGAGAGGACACCTCTCATGGTTGCAGCTATGTATGGAAGCTTGGATGTATCGGCTTATATTCTTGGAACAGGTCGGGTTGATGTTAATCGGTCTAGTGGATCCGATGGTGCTACTGCTCTTCATTGTGCGGTTGTTGGGGGTTCTGCTGCTTCCCCCGAGATTATCAAGATTTTGCTTGATGCATCGGCGGATGCTAGTGCTGTTGATGCCAACGGTAACCGACCAGTTGATTTGATTGTTTCGCTGGCCAATTCTATCTTCAATCCAAGGAAGAGGATGCTACAAGCTCTTTTGGATGGTACAGACGGTGCTGATCAGACATGTCTTCTGTTTCCGGAGACAATTGATGACATTGACGAATATCAAAGGCAAGATGTAAACACGCCTCGTGTTTCAAAAGATTATCCTGTTGATGTCTCTCTTCCAGACATTAAGAATGGGATATATAGTACCGATGAGTTTAGGATGTATACATTCAAAGTGAAGCCTTGTTCAAGGGCATATTCTCATGACTGGACCGAGTGTCCCTTTGTTCATCCAGGGGAAAATGCAAGGCGACGGGATCCAAGAAAATATCATTATAGCTGCGTCCCTTGTCCTGAGTTTCGGAAAGGATCGTGCAGTAAAGGAGATTCTTGTGACTATGCACACGGTATTTTTGAGTGCTGGCTTCATCCTGCTCAGTATAAGACACGGCTTTGCAAGGATGAAGGTTCATGCATGAGAAGAGTTTGCTTCTTTGCTCACAAGGTCGAGGAGCTTCGCCCCTTGTATGCTTCTACCGGTTCTGCTATTCCATCTCCTAGATCATATTACAGTACTGCTTCTACATTAGAGATGGGTTCAATTAGCCCAATGTCACTTGGTTCTCCATCTGTTTTGATACCACCCTCCTCAACGCCGCCTTTGACTTCATCTGGAGCATCGTCTCCCGTTGCTGCTGCAGCCATGTGGCAGACACCGTCTAATGTTTCGATCCCTACCCTTCAGCTTCCCAAAAGCAGATTGAAGACTGGAATGACCGCTAGAGATATTAATTCAGATATTGCAATGCTTAGGGTTGAAACTCAGCGGCGAAAGCAGCAACTACTGATGGATGAGATGGCCGGTCTTTCCTCTCCTTCCAACTGGAATCATTCCATGCCTAACAGTCCATCTTTTTCGGTTTCTTCGACCAACCATACGACCGGGGAACTAAATAGGTTTTCGGGGGTGAATCCTACAAACCTTGAAGACATTTTCGGGTCTCTTGATCCATCAATGTTGCATAAATTCCACAGAATTTCACTTGATGTGGCAGGAGCAGCAGGATCTCAGTTACAATCTCCGACTGGAATCCAAATGCGTCCGAATATGAATCAGCATCTGCAACAAAACTATGGCCATTCCACTTCCAGTGCGATTGGATCTCCGACCTATAGGTTTGATCCATCTGGGGAGTTATCCGCGTCGGCTTTGAATGCAAGAGCTGCTGCATTTTCAAAGCGGAGCCAGAGCTTTATCGAGCGCGGTGTCACAAACCGTCATTCTGAGCTTCATTCTCCTGCTAAGCCTTATGCTTTTTCTAACTGGGGCTCATCCGATGGAAATTTGGATTGGACTTCACACGGAGAAGAACTGAATAAGCTGAGGAAATCATCTTCATTTGGATTTCGAACCACCAGTACTCCTTTAACTCCAGCTGCAGCCAGGGCACAGGAAAATGACTATGAACCAGATGTGTCTTGGGTTAATTCACTTGTAAAGGATGCTACGCCGCAAGAATCTCATCAGTTTAGCGTTGAAGATCAGACAAGGAAACTGCAGCGCCATCTTAACGGAACAGATTCGATTCCGGCTTGGTTGGAGCAATTGTACATGGATCAGGAGCAAATAGTGCAGTGA

Protein sequence

>MsaT028689.1

MCSDSKKKTCQKDLIMGCEYVNGNKEGQYHEVSYLLEFSAADDVVGLKNAIENEGCDVDGVGLWYGRKVGSNKLGYEERTPLMVAAMYGSLDVSAYILGTGRVDVNRSSGSDGATALHCAVVGGSAASPEIIKILLDASADASAVDANGNRPVDLIVSLANSIFNPRKRMLQALLDGTDGADQTCLLFPETIDDIDEYQRQDVNTPRVSKDYPVDVSLPDIKNGIYSTDEFRMYTFKVKPCSRAYSHDWTECPFVHPGENARRRDPRKYHYSCVPCPEFRKGSCSKGDSCDYAHGIFECWLHPAQYKTRLCKDEGSCMRRVCFFAHKVEELRPLYASTGSAIPSPRSYYSTASTLEMGSISPMSLGSPSVLIPPSSTPPLTSSGASSPVAAAAMWQTPSNVSIPTLQLPKSRLKTGMTARDINSDIAMLRVETQRRKQQLLMDEMAGLSSPSNWNHSMPNSPSFSVSSTNHTTGELNRFSGVNPTNLEDIFGSLDPSMLHKFHRISLDVAGAAGSQLQSPTGIQMRPNMNQHLQQNYGHSTSSAIGSPTYRFDPSGELSASALNARAAAFSKRSQSFIERGVTNRHSELHSPAKPYAFSNWGSSDGNLDWTSHGEELNKLRKSSSFGFRTTSTPLTPAAARAQENDYEPDVSWVNSLVKDATPQESHQFSVEDQTRKLQRHLNGTDSIPAWLEQLYMDQEQIVQ*