Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028707 | AES61431.2 | 92.045 | 176 | 14 | 0 | 1 | 176 | 35 | 210 | 3.49e-117 | 344 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028707 | tr|G7I8T1|G7I8T1_MEDTR | 92.045 | 176 | 14 | 0 | 1 | 176 | 35 | 210 | 1.67e-117 | 344 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG028707 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028707 | MtrunA17_Chr1g0172971 | 85.714 | 98 | 14 | 0 | 1 | 98 | 20 | 117 | 1.95e-56 | 186 |
| MsaG028707 | MtrunA17_Chr1g0202341 | 29.814 | 161 | 91 | 5 | 16 | 174 | 50 | 190 | 3.07e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTTGTTGGCCTGTTGTT+AGG | 0.292589 | 5:+67481872 | None:intergenic |
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 5:+67481681 | None:intergenic |
| CGTTATGTTTGTAACAAAGA+AGG | 0.427485 | 5:-67481920 | MsaT028707.1:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 5:-67481668 | MsaT028707.1:CDS |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 5:+67482083 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 5:+67482082 | None:intergenic |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 5:-67481803 | MsaT028707.1:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.452819 | 5:-67481751 | MsaT028707.1:CDS |
| TGAGAACGTCTTTGAAGCTA+TGG | 0.454271 | 5:-67481607 | MsaT028707.1:CDS |
| GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 5:-67481638 | MsaT028707.1:CDS |
| CTAAGGTAACGCGATTGCTC+TGG | 0.466250 | 5:+67481698 | None:intergenic |
| GTTTACTTAATCCTAACAAC+AGG | 0.470860 | 5:-67481883 | MsaT028707.1:CDS |
| GGTAATTATGCATATAACAT+TGG | 0.471419 | 5:-67482016 | MsaT028707.1:CDS |
| TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 5:+67481555 | None:intergenic |
| GAAGCTATGGGTCAAGGAGT+TGG | 0.491130 | 5:-67481594 | MsaT028707.1:CDS |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 5:-67482084 | MsaT028707.1:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 5:+67481809 | None:intergenic |
| AGCTGGAATATATTTGAAGC+TGG | 0.507410 | 5:-67481786 | MsaT028707.1:CDS |
| ACAAAACAAGAAATCTGATA+AGG | 0.511435 | 5:-67481954 | MsaT028707.1:CDS |
| ACCAGTGGACTGTCTGTTGT+TGG | 0.515301 | 5:+67481860 | None:intergenic |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.525236 | 5:-67481909 | MsaT028707.1:CDS |
| GATTGCTCTGGTTTATGTAA+TGG | 0.525874 | 5:+67481710 | None:intergenic |
| TAAGATATATAGATGGGATG+TGG | 0.528498 | 5:-67481744 | MsaT028707.1:CDS |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 5:+67482084 | None:intergenic |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 5:-67481908 | MsaT028707.1:CDS |
| GTCTTTGAAGCTATGGGTCA+AGG | 0.563657 | 5:-67481600 | MsaT028707.1:CDS |
| GCTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 5:-67481591 | MsaT028707.1:CDS |
| TGTGTATAAGATATATAGAT+GGG | 0.601002 | 5:-67481750 | MsaT028707.1:CDS |
| GGGTCAAGGAGTTGGAGGCA+TGG | 0.604978 | 5:-67481586 | MsaT028707.1:CDS |
| GCCAACAACAGACAGTCCAC+TGG | 0.611268 | 5:-67481861 | MsaT028707.1:CDS |
| TTCTCAAACTCAATAACCAG+TGG | 0.616706 | 5:+67481845 | None:intergenic |
| GAGAACGTCTTTGAAGCTAT+GGG | 0.624783 | 5:-67481606 | MsaT028707.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 67481556 | 67482137 | 67481556 | ID=MsaG028707 |
| Chr5 | mRNA | 67481556 | 67482137 | 67481556 | ID=MsaT028707.1;Parent=MsaG028707 |
| Chr5 | exon | 67481556 | 67482137 | 67481556 | ID=MsaT028707.1.exon1;Parent=MsaT028707.1 |
| Chr5 | CDS | 67481556 | 67482137 | 67481556 | ID=cds.MsaT028707.1;Parent=MsaT028707.1 |
| Gene Sequence |
| Protein sequence |
