AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028711

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028711 MtrunA17_Chr5g0430361 94.752 1067 34 1 32 1076 5 1071 0.0 2086
MsaG028711 MtrunA17_Chr3g0105291 46.892 1126 494 20 21 1118 11 1060 0.0 970
MsaG028711 MtrunA17_Chr5g0407211 43.155 431 206 9 657 1062 3 419 7.88e-112 355
MsaG028711 MtrunA17_Chr5g0407251 42.691 431 208 9 657 1062 3 419 1.42e-110 351
MsaG028711 MtrunA17_Chr7g0270101 44.578 415 212 8 656 1062 383 787 8.41e-110 361
MsaG028711 MtrunA17_Chr3g0134821 39.831 472 247 10 615 1064 378 834 1.60e-100 337
MsaG028711 MtrunA17_Chr3g0134811 35.928 501 245 9 626 1064 307 793 1.81e-99 333
MsaG028711 MtrunA17_Chr4g0012501 54.941 253 103 5 6 253 3 249 1.87e-84 274
MsaG028711 MtrunA17_Chr2g0316251 35.152 495 300 10 576 1062 685 1166 6.68e-81 290
MsaG028711 MtrunA17_Chr1g0205451 34.592 503 296 10 568 1063 537 1013 9.34e-75 272
MsaG028711 MtrunA17_Chr1g0185491 33.203 512 302 14 598 1090 760 1250 1.16e-74 271
MsaG028711 MtrunA17_Chr7g0259981 33.333 324 183 8 751 1070 6 300 1.16e-54 193
MsaG028711 MtrunA17_Chr7g0265211 29.139 453 229 11 680 1062 517 947 3.11e-48 187
MsaG028711 MtrunA17_Chr1g0182481 29.087 416 230 10 694 1062 496 893 3.58e-47 183
MsaG028711 MtrunA17_Chr1g0179841 29.911 448 224 13 686 1064 378 804 3.15e-44 173
MsaG028711 MtrunA17_Chr3g0134851 33.333 402 184 12 697 1073 129 471 6.39e-44 166
MsaG028711 MtrunA17_Chr4g0055731 26.316 418 241 16 655 1062 109 469 8.52e-31 127
MsaG028711 MtrunA17_Chr4g0028111 24.433 397 213 9 705 1035 185 560 3.64e-29 124
MsaG028711 MtrunA17_Chr4g0027811 24.178 426 209 12 690 1063 216 579 4.08e-28 121
MsaG028711 MtrunA17_Chr2g0320451 31.064 235 140 6 841 1067 808 1028 1.86e-22 104
MsaG028711 MtrunA17_Chr2g0320451 25.379 264 132 6 694 907 500 748 5.35e-18 90.1
MsaG028711 MtrunA17_Chr4g0025251 25.000 260 170 6 823 1063 449 702 8.09e-21 98.6
MsaG028711 MtrunA17_Chr3g0101951 34.028 144 88 5 26 167 395 533 7.56e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028711 AT5G58610.3 37.422 1117 596 28 12 1106 14 1049 0.0 670
MsaG028711 AT5G58610.8 38.140 1075 565 27 12 1064 14 1010 0.0 669
MsaG028711 AT5G58610.2 38.140 1075 565 27 12 1064 14 1010 0.0 669
MsaG028711 AT5G58610.5 37.376 1105 589 28 24 1106 17 1040 0.0 657
MsaG028711 AT5G58610.7 37.376 1105 589 28 24 1106 17 1040 0.0 656
MsaG028711 AT5G58610.6 36.561 1105 572 30 24 1106 17 1014 0.0 623
MsaG028711 AT5G58610.1 36.561 1105 572 30 24 1106 17 1014 0.0 622
MsaG028711 AT5G58610.10 36.925 1021 544 27 12 1010 14 956 0.0 600
MsaG028711 AT5G58610.9 36.868 1009 537 27 24 1010 17 947 0.0 586
MsaG028711 AT5G58610.11 36.045 971 521 27 24 972 17 909 1.87e-174 536
MsaG028711 AT5G58610.4 36.275 816 445 21 301 1106 68 818 9.03e-145 457
MsaG028711 AT3G14980.3 35.931 462 288 6 614 1071 575 1032 1.82e-85 300
MsaG028711 AT3G14980.5 35.931 462 288 6 614 1071 530 987 2.82e-85 301
MsaG028711 AT3G14980.6 35.931 462 288 6 614 1071 575 1032 4.85e-85 301
MsaG028711 AT3G14980.1 35.931 462 288 6 614 1071 575 1032 4.85e-85 301
MsaG028711 AT3G14980.2 35.931 462 288 6 614 1071 575 1032 4.85e-85 301
MsaG028711 AT1G05380.3 38.609 417 246 7 651 1062 514 925 8.52e-84 296
MsaG028711 AT1G05380.1 38.609 417 246 7 651 1062 514 925 8.52e-84 296
MsaG028711 AT1G05380.2 38.609 417 246 7 651 1062 514 925 8.52e-84 296
MsaG028711 AT5G36740.5 34.968 469 279 9 611 1073 512 960 1.82e-82 293
MsaG028711 AT5G36740.1 34.968 469 279 9 611 1073 512 960 1.82e-82 293
MsaG028711 AT5G36740.4 34.029 479 280 10 611 1073 359 817 5.10e-79 281
MsaG028711 AT4G14920.2 35.308 439 272 8 655 1088 578 1009 1.11e-73 267
MsaG028711 AT4G14920.1 35.308 439 272 8 655 1088 578 1009 1.11e-73 267
MsaG028711 AT4G14920.3 35.455 440 271 9 655 1088 578 1010 4.46e-73 265
MsaG028711 AT5G63900.1 43.464 306 152 9 762 1064 255 542 7.67e-71 247
MsaG028711 AT5G36670.4 32.165 485 285 11 611 1073 512 974 5.14e-70 256
MsaG028711 AT5G36670.5 32.165 485 285 11 611 1073 512 974 5.14e-70 256
MsaG028711 AT5G36670.2 34.500 400 236 9 611 1004 512 891 7.45e-67 243
MsaG028711 AT5G36740.2 34.500 400 236 9 611 1004 512 891 7.45e-67 243
MsaG028711 AT5G36740.3 34.500 400 236 9 611 1004 512 891 7.45e-67 243
MsaG028711 AT5G36670.3 31.313 495 286 12 611 1073 359 831 1.51e-66 244
MsaG028711 AT5G36670.1 31.313 495 286 12 611 1073 512 984 4.92e-66 244
MsaG028711 AT3G14980.4 34.341 364 231 6 614 973 575 934 1.67e-58 219
MsaG028711 AT2G27980.1 25.724 587 341 14 563 1080 512 1072 3.73e-47 184
MsaG028711 AT2G37520.1 28.605 430 234 9 686 1060 402 813 1.42e-46 181
MsaG028711 AT3G53680.1 27.500 440 249 10 686 1072 419 841 2.58e-45 177
MsaG028711 AT3G53680.2 27.500 440 249 10 686 1072 421 843 2.89e-45 177
MsaG028711 AT2G36720.1 27.107 439 224 10 694 1060 557 971 8.20e-44 173
MsaG028711 AT2G36720.2 27.107 439 224 10 694 1060 557 971 8.20e-44 173

Find 253 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGCTTTCCTAATAGATTT+TGG 0.135803 5:-67546637 None:intergenic
TCATAAGAATTGTCTTAATT+TGG 0.158903 5:+67545689 MsaT028711.1:CDS
CGTTGAAACCTTGGCTCATT+TGG 0.168863 5:-67544753 None:intergenic
TAAATTGTTTGGAAGGTTTA+AGG 0.204622 5:-67544080 None:intergenic
TTCCAAAGGCTTCCAAATTA+TGG 0.219352 5:-67544422 None:intergenic
CAAAAGGGTGCAGAAGGTTT+TGG 0.232594 5:+67545254 MsaT028711.1:CDS
TTCATTTCATCACCTAAATC+TGG 0.258138 5:-67543776 None:intergenic
TTGAAGTCCTTGTGCGTTTA+CGG 0.261009 5:-67545173 None:intergenic
GACCTTGTTGTTGAGGTAAT+TGG 0.281049 5:+67544019 MsaT028711.1:CDS
GGTTCAACTTGAAATTTAAC+TGG 0.282801 5:-67544802 None:intergenic
AGAAAACATCTTGTTTATCT+TGG 0.286222 5:+67544538 MsaT028711.1:CDS
GGATAACTCGCGAAGGAATT+AGG 0.287893 5:+67545400 MsaT028711.1:CDS
AGCATATTCTTCCCAGATTT+AGG 0.288962 5:+67543764 MsaT028711.1:CDS
GTTGAAACCTTGGCTCATTT+GGG 0.293407 5:-67544752 None:intergenic
GACGTTCTAAGTCCATAATT+TGG 0.302733 5:+67544410 MsaT028711.1:CDS
ACGGGGCTCCAAAATCTATT+AGG 0.304902 5:+67546629 MsaT028711.1:CDS
GGTGGATGTTTCTAATGTTT+TGG 0.305903 5:+67543559 MsaT028711.1:CDS
TCCAATTGAGTTACGGATAA+AGG 0.306009 5:+67543649 MsaT028711.1:CDS
AAGTTACCATCTTGTTGTTT+TGG 0.312082 5:-67548600 None:intergenic
GTCCATAATTTGGAAGCCTT+TGG 0.322129 5:+67544420 MsaT028711.1:CDS
GCTTGATCCACTTCAGATAC+TGG 0.326871 5:-67548660 None:intergenic
TTTGTCCATCATGCCGTTGC+GGG 0.334836 5:+67545876 MsaT028711.1:CDS
ATTACAAGTCCATTCTCTAA+TGG 0.340868 5:-67543978 None:intergenic
CCTCTTGTTACAACCGATTC+CGG 0.341704 5:-67548361 None:intergenic
CTAGTCTTGCCTGCTGTTCC+CGG 0.346824 5:+67548202 MsaT028711.1:CDS
GTGATAATGGAAATCGAAAA+AGG 0.351320 5:+67542811 MsaT028711.1:CDS
TCCTTTATCCGTAACTCAAT+TGG 0.356636 5:-67543650 None:intergenic
CGGATAAAGGATCTTCCATT+TGG 0.359722 5:+67543662 MsaT028711.1:CDS
GACACATGGACGAATTCTTT+TGG 0.363350 5:+67548232 MsaT028711.1:CDS
CGCCGACTCCTCAAACTTGT+TGG 0.370823 5:+67544210 MsaT028711.1:CDS
TCATCAGAAGCTAAAGCAAC+TGG 0.372787 5:+67548168 MsaT028711.1:intron
AACAGATTTGGTATGATATA+AGG 0.374438 5:+67543933 MsaT028711.1:CDS
CCATCTATAGTACGTAATTC+AGG 0.376584 5:-67545126 None:intergenic
GAATGATGGATCTACAAGTT+TGG 0.392714 5:+67548703 MsaT028711.1:CDS
GATGGTGATAAGAGTGATAA+TGG 0.395193 5:+67542798 MsaT028711.1:CDS
ATTGGTTACCAAGAGGGAAA+TGG 0.396210 5:+67543852 MsaT028711.1:CDS
GGTGGTCCTGAGTTCATGTT+AGG 0.398485 5:-67544688 None:intergenic
AACTTTGGATTAAGTTTATC+CGG 0.400564 5:-67544622 None:intergenic
ACCTGTTGTCAGAATCTATA+TGG 0.400741 5:+67545426 MsaT028711.1:CDS
CGTAGGAGAGTTTCCTCCTT+CGG 0.403737 5:-67545367 None:intergenic
AAAATCGAAGTGTAAAACTT+TGG 0.406804 5:-67544637 None:intergenic
CAGTAAAAGACCTTGTACTT+TGG 0.409730 5:-67545339 None:intergenic
ATGTAGGAGCAAGACGGTTC+GGG 0.411437 5:+67544495 MsaT028711.1:CDS
AAGTCGTCTGTACTGAAATC+TGG 0.412571 5:-67547953 None:intergenic
GCTAATCAAGGCGATGCATT+TGG 0.413138 5:+67544151 MsaT028711.1:CDS
ATGATCAGAATTGGTCCATT+CGG 0.414801 5:-67545052 None:intergenic
ATGGCAAGAGGACAAAATTC+TGG 0.415798 5:-67544823 None:intergenic
TATGATACGCCGAGGGGCAT+TGG 0.416757 5:-67545671 None:intergenic
CAGCACAAGCATCATTCCTT+TGG 0.417068 5:+67544252 MsaT028711.1:CDS
CGGTTTCTCTTTGCAGTTGG+TGG 0.417342 5:-67545153 None:intergenic
GGGATGAATCAACTGAGAAT+TGG 0.419006 5:+67543834 MsaT028711.1:CDS
AACATCTTGTTTATCTTGGA+TGG 0.419268 5:+67544542 MsaT028711.1:CDS
TATTCTTTCCTAGACTTTCA+AGG 0.420955 5:+67548298 MsaT028711.1:CDS
GCTCAATCGACTAAACTTCC+AGG 0.421981 5:+67547726 MsaT028711.1:CDS
TGCTAGAGAAAGGGTTATGC+AGG 0.423833 5:-67546811 None:intergenic
TCTCCCTCGAGTAAATTGTT+TGG 0.424533 5:-67544091 None:intergenic
ATCACCACCATGATCAGAAT+TGG 0.425092 5:-67545061 None:intergenic
GATGTAGGAGCAAGACGGTT+CGG 0.425946 5:+67544494 MsaT028711.1:CDS
CCTGAATTACGTACTATAGA+TGG 0.427897 5:+67545126 MsaT028711.1:CDS
ATCAGTAACCAACAAGTTTG+AGG 0.429317 5:-67544218 None:intergenic
CAGTCTCGGTGAAACAGATT+TGG 0.429902 5:+67543921 MsaT028711.1:CDS
TGATCAGAATTGGTCCATTC+GGG 0.429936 5:-67545051 None:intergenic
TTGGTTACCAAGAGGGAAAT+GGG 0.430226 5:+67543853 MsaT028711.1:CDS
AGGGCATCCCTGATGGTGAT+TGG 0.430704 5:+67545852 MsaT028711.1:intron
ATCTTGGATGGACAATTGAA+TGG 0.433717 5:+67544554 MsaT028711.1:CDS
ATTTGTTCTGTTTGTAACTA+CGG 0.435651 5:+67545627 MsaT028711.1:CDS
GAGGCGGTAACGGTCTAATC+AGG 0.443329 5:-67543628 None:intergenic
AACTCAATTGGAGGCGGTAA+CGG 0.443516 5:-67543638 None:intergenic
ATGTGTTGATGTGAAATATC+AGG 0.443715 5:+67543688 MsaT028711.1:CDS
TTTAACTGGAGAAGGTTCAT+TGG 0.444310 5:-67544788 None:intergenic
TCCATATAGATTCTGACAAC+AGG 0.445023 5:-67545427 None:intergenic
GGAAACTCTCCTACGTTTGA+AGG 0.447090 5:+67545375 MsaT028711.1:CDS
ACATCAACACATTGTCCAAA+TGG 0.448928 5:-67543677 None:intergenic
AAGATTTCCATCTCCGGAAT+CGG 0.449395 5:+67548348 MsaT028711.1:CDS
TCTCCACAAGATCAGTGTTT+AGG 0.452938 5:+67544985 MsaT028711.1:CDS
GCATCATTCCTTTGGTGGAT+GGG 0.455178 5:+67544260 MsaT028711.1:CDS
AACTCTGAGACTTGCGGTGT+TGG 0.458093 5:+67546707 MsaT028711.1:CDS
CGATCGCTGCGATGGAATGA+AGG 0.459419 5:+67543736 MsaT028711.1:CDS
GAAGCTAAAGCAACTGGGAG+TGG 0.459529 5:+67548174 MsaT028711.1:intron
AGCCTCAAAATATTCTGTCT+TGG 0.464847 5:+67545292 MsaT028711.1:CDS
CAGTTGAGGAGTTTGGAAGA+TGG 0.465345 5:+67543425 MsaT028711.1:intron
CAAACTGAGTGTTGCACTCT+CGG 0.468934 5:+67546766 MsaT028711.1:CDS
CCGCACAATGATCTGTTTGA+AGG 0.470390 5:+67545591 MsaT028711.1:CDS
GGACAAAACCAATCACCATC+AGG 0.470956 5:-67545860 None:intergenic
TTACGGTTTCTCTTTGCAGT+TGG 0.473232 5:-67545156 None:intergenic
TTTCTGGGATCATGGCATCC+AGG 0.474048 5:+67543449 MsaT028711.1:CDS
GTAGTCTTCCTTCTTTCAGT+TGG 0.476165 5:-67544952 None:intergenic
CATCATTCCTTTGGTGGATG+GGG 0.477185 5:+67544261 MsaT028711.1:CDS
ACGATATCTCTGCTAGAGAA+AGG 0.477387 5:-67546821 None:intergenic
TCTTCATCTGCTTTAGATTG+TGG 0.478088 5:+67543593 MsaT028711.1:CDS
GGGTGCTAAGATCAAGCAAA+AGG 0.478848 5:+67545238 MsaT028711.1:CDS
TAAGAGAAAGTGGAAATTGA+AGG 0.482244 5:+67544888 MsaT028711.1:CDS
GCATACTCATCAATAACTTC+AGG 0.482703 5:-67544466 None:intergenic
CACAAGCATCATTCCTTTGG+TGG 0.482854 5:+67544255 MsaT028711.1:CDS
TTGCTGAAACCATTAGAGAA+TGG 0.483151 5:+67543969 MsaT028711.1:CDS
TCTGAGACTTGCGGTGTTGG+CGG 0.484707 5:+67546710 MsaT028711.1:CDS
AGCATCATTCCTTTGGTGGA+TGG 0.485615 5:+67544259 MsaT028711.1:CDS
TTTCACCGAGACTGCAACAT+AGG 0.485786 5:-67543912 None:intergenic
GTGATATTCGATCGCTGCGA+TGG 0.486249 5:+67543728 MsaT028711.1:CDS
ACTGGAGAAGGTTCATTGGT+TGG 0.487564 5:-67544784 None:intergenic
GAGGACAAACGTTGAAACCT+TGG 0.487775 5:-67544762 None:intergenic
AACTGAGGATCACTCAGGAT+TGG 0.488881 5:+67543813 MsaT028711.1:CDS
ACTTGAAATTTAACTGGAGA+AGG 0.489209 5:-67544796 None:intergenic
TTAGGTGATGAAATGAAGGT+TGG 0.489849 5:+67543782 MsaT028711.1:CDS
TAGTCTTCCTTCTTTCAGTT+GGG 0.489926 5:-67544951 None:intergenic
TTCAAAGGCTAGCACACAAA+AGG 0.490434 5:+67545191 MsaT028711.1:CDS
CATTGTGACGCTGCTAATCA+AGG 0.490750 5:+67544139 MsaT028711.1:CDS
GATTAGCAAAAGAGGAATGA+TGG 0.492433 5:+67548689 MsaT028711.1:CDS
CATTGTGTTGCCAAAGTACA+AGG 0.495043 5:+67545329 MsaT028711.1:CDS
ACTGAGGATCACTCAGGATT+GGG 0.495646 5:+67543814 MsaT028711.1:CDS
CAACCATCAATGGAGGTTGA+TGG 0.496477 5:+67542780 None:intergenic
CCTCGAGTAAATTGTTTGGA+AGG 0.499084 5:-67544087 None:intergenic
GTTACAACCGATTCCGGAGA+TGG 0.499267 5:-67548355 None:intergenic
CAGTGTTTAGGCACACTTCA+AGG 0.501569 5:+67544997 MsaT028711.1:CDS
AAACCTTGGCTCATTTGGGG+TGG 0.502467 5:-67544748 None:intergenic
CCAAAATCTATTAGGAAAGC+CGG 0.502943 5:+67546637 MsaT028711.1:CDS
GGTATCCTATGTTGCAGTCT+CGG 0.503297 5:+67543907 MsaT028711.1:CDS
TACCGCCTCCAATTGAGTTA+CGG 0.506527 5:+67543642 MsaT028711.1:CDS
AGATTTAGGTGATGAAATGA+AGG 0.508571 5:+67543778 MsaT028711.1:CDS
GTATGATATAAGGATGAGAA+AGG 0.514448 5:+67543943 MsaT028711.1:CDS
CAAATCCCGCAACGGCATGA+TGG 0.514646 5:-67545881 None:intergenic
TTTATCCGTAACTCAATTGG+AGG 0.515632 5:-67543647 None:intergenic
TAAGAATTGTCTTAATTTGG+AGG 0.523841 5:+67545692 MsaT028711.1:CDS
GCAGAAGGGATTAGCAAAAG+AGG 0.524390 5:+67548681 MsaT028711.1:CDS
TCATTTCATCACCTAAATCT+GGG 0.526675 5:-67543775 None:intergenic
AGCGGCTTTGCAAACCATGC+TGG 0.527028 5:+67545453 MsaT028711.1:CDS
TTCTGGGATCATGGCATCCA+GGG 0.527252 5:+67543450 MsaT028711.1:CDS
TTTAATGGATGAGCTCGAAA+AGG 0.528962 5:+67547990 MsaT028711.1:CDS
GAATGGACCAATTCTGATCA+TGG 0.531149 5:+67545054 MsaT028711.1:CDS
TCTAGCACACCGGGAACAGC+AGG 0.531932 5:-67548211 None:intergenic
CGATATCTCTGCTAGAGAAA+GGG 0.534298 5:-67546820 None:intergenic
ATCAAGCAAAAGGGTGCAGA+AGG 0.536140 5:+67545248 MsaT028711.1:CDS
GAAAGATTGCTGTCAATTTG+AGG 0.537617 5:-67544709 None:intergenic
CTGTGGAGAAGAGTGTGAAC+GGG 0.542047 5:+67546167 MsaT028711.1:CDS
AACATCTGTTAGCAGAAGGA+TGG 0.542192 5:+67544917 MsaT028711.1:CDS
AACCAACAAGTTTGAGGAGT+CGG 0.543763 5:-67544212 None:intergenic
AGATTTCAGTACAGACGACT+TGG 0.545529 5:+67547955 MsaT028711.1:CDS
GTTGACAAGATTTCCATCTC+CGG 0.546423 5:+67548342 MsaT028711.1:CDS
TATCAGGATGCTTGGTGGGA+AGG 0.546580 5:+67543704 MsaT028711.1:CDS
ATCAGATTATCTTATTGAAG+TGG 0.547552 5:+67543538 MsaT028711.1:CDS
GGACAATCTAACTTGGACAT+TGG 0.549998 5:+67546673 MsaT028711.1:CDS
CTGAGAATTGGTTACCAAGA+GGG 0.550005 5:+67543846 MsaT028711.1:CDS
TTGTTTGGAAGGTTTAAGGC+GGG 0.550395 5:-67544076 None:intergenic
GTTTATCCGGAGACTTGTAC+CGG 0.551483 5:-67544609 None:intergenic
TGACAGTTCCTTGAAAGTCT+AGG 0.552331 5:-67548306 None:intergenic
ACTCGGCTGATAAGAGAAAG+TGG 0.552574 5:+67544878 MsaT028711.1:CDS
AAGACGTTATATTCAACCAA+AGG 0.553521 5:+67546846 MsaT028711.1:CDS
AGTTACTGCTTCCACCAGCA+TGG 0.554079 5:-67545467 None:intergenic
ACGATACCGGTACAAGTCTC+CGG 0.556383 5:+67544603 MsaT028711.1:CDS
AATCTGATTCTTCATCATCA+AGG 0.558197 5:-67543523 None:intergenic
GTCCATGTGTCTAGCACACC+GGG 0.558868 5:-67548220 None:intergenic
TTCCCGGTGTGCTAGACACA+TGG 0.559676 5:+67548218 MsaT028711.1:CDS
TGCTGTAATTCTCCGCCACT+AGG 0.560357 5:-67546745 None:intergenic
ATCCGTAACTCAATTGGAGG+CGG 0.563021 5:-67543644 None:intergenic
CATGTGTAGGGCATCCCTGA+TGG 0.563743 5:+67545845 MsaT028711.1:intron
GTGCCTAAACACTGATCTTG+TGG 0.566474 5:-67544988 None:intergenic
CTCAATCGACTAAACTTCCA+GGG 0.567209 5:+67547727 MsaT028711.1:CDS
TCAACTTCAGACAGTTCCAA+AGG 0.567236 5:-67544436 None:intergenic
TTGACTACCCAACTGAAAGA+AGG 0.567692 5:+67544944 MsaT028711.1:CDS
AGATTATCTTATTGAAGTGG+TGG 0.568142 5:+67543541 MsaT028711.1:CDS
GCTGCGATGGAATGAAGGAT+AGG 0.569938 5:+67543741 MsaT028711.1:CDS
TCACAATGAACATTATCAGT+TGG 0.571207 5:-67544124 None:intergenic
GGCTAGTCCAGTATCTGAAG+TGG 0.575225 5:+67548653 MsaT028711.1:CDS
CGTCCATGTGTCTAGCACAC+CGG 0.577703 5:-67548221 None:intergenic
GAAACTCTCCTACGTTTGAA+GGG 0.580720 5:+67545376 MsaT028711.1:CDS
GGTGCTAAGATCAAGCAAAA+GGG 0.582852 5:+67545239 MsaT028711.1:CDS
CCGGAATCGGTTGTAACAAG+AGG 0.584259 5:+67548361 MsaT028711.1:CDS
GAAGTACAGTATAGAAACCC+TGG 0.584288 5:-67547744 None:intergenic
CATCAGAAGCTAAAGCAACT+GGG 0.584545 5:+67548169 MsaT028711.1:intron
GGAGAAGGTTCATTGGTTGG+AGG 0.585791 5:-67544781 None:intergenic
GTGGAGAGACCTTGTTGTTG+AGG 0.588535 5:+67544012 MsaT028711.1:CDS
GCTGTTTGAGGAGTGTGAGA+GGG 0.589438 5:+67543886 MsaT028711.1:CDS
AGTAAGAAAGTGTCCATCCT+CGG 0.590389 5:-67545927 None:intergenic
ATGTGAAATATCAGGATGCT+TGG 0.590726 5:+67543696 MsaT028711.1:CDS
GCATGGTTTGCAAAGCCGCT+AGG 0.591498 5:-67545450 None:intergenic
TTGAAACCTTGGCTCATTTG+GGG 0.593251 5:-67544751 None:intergenic
GTTATTGATGAGTATGCACT+TGG 0.594005 5:+67544472 MsaT028711.1:CDS
AGAATGGACTTGTAATGTGA+AGG 0.594117 5:+67543985 MsaT028711.1:CDS
GAAATATCAGGATGCTTGGT+GGG 0.594887 5:+67543700 MsaT028711.1:CDS
CTTCCAAACAATTTACTCGA+GGG 0.595130 5:+67544088 MsaT028711.1:CDS
GGCGAACTTCGCTTAATGTG+AGG 0.596695 5:-67544054 None:intergenic
TTTATGCGACCAATGCCCCT+CGG 0.597093 5:+67545662 MsaT028711.1:CDS
GCGAACTTCGCTTAATGTGA+GGG 0.597250 5:-67544053 None:intergenic
AAATGAAGAATTGATTAGTG+TGG 0.598410 5:+67547774 MsaT028711.1:CDS
CTCCTCCACATAGACGATAC+CGG 0.598432 5:+67544590 MsaT028711.1:CDS
AAAATTGAAGAGACCGAGGA+TGG 0.598542 5:+67545914 MsaT028711.1:CDS
ATGAGTATGCACTTGGATGT+AGG 0.598658 5:+67544479 MsaT028711.1:CDS
TGAAATATCAGGATGCTTGG+TGG 0.599189 5:+67543699 MsaT028711.1:CDS
GGGAAGTAATGCAAGATCAC+AGG 0.600203 5:+67545547 MsaT028711.1:CDS
TCTGAAGTGGATCAAGCAGA+AGG 0.601234 5:+67548666 MsaT028711.1:CDS
CAGGGAAAATTATCCGCTGT+GGG 0.601586 5:+67543468 MsaT028711.1:CDS
TTTGGCTGCAAATCCCGCAA+CGG 0.603767 5:-67545889 None:intergenic
TAGTATTTCACGATGGCAAG+AGG 0.605840 5:-67544835 None:intergenic
CCTTCCAAACAATTTACTCG+AGG 0.608045 5:+67544087 MsaT028711.1:CDS
GGTGGATGGGGAATCTGAGA+AGG 0.612895 5:+67544273 MsaT028711.1:CDS
TCACAGGACAAGAGAAATCA+CGG 0.613557 5:+67545563 MsaT028711.1:CDS
TTGTACCGGTATCGTCTATG+TGG 0.615054 5:-67544595 None:intergenic
CCAGGGAAAATTATCCGCTG+TGG 0.615566 5:+67543467 MsaT028711.1:CDS
TAAACGCACAAGGACTTCAA+AGG 0.618492 5:+67545176 MsaT028711.1:CDS
TACCGGTATCGTCTATGTGG+AGG 0.619310 5:-67544592 None:intergenic
GGTCACTGTTAGATTGCATG+AGG 0.622465 5:+67545526 MsaT028711.1:CDS
CACCAATTACCTCAACAACA+AGG 0.625256 5:-67544021 None:intergenic
GAGGATCCTAACATGAACTC+AGG 0.625620 5:+67544682 MsaT028711.1:CDS
GCTAGAGAAAGGGTTATGCA+GGG 0.625919 5:-67546810 None:intergenic
CTGAAGTGGATCAAGCAGAA+GGG 0.627799 5:+67548667 MsaT028711.1:CDS
TCAGATTCCCCATCCACCAA+AGG 0.629870 5:-67544268 None:intergenic
TGCAATATCTGATAAGCCTG+AGG 0.631201 5:+67544183 MsaT028711.1:CDS
CTTGTAATGTGAAGGAGTTG+TGG 0.634145 5:+67543993 MsaT028711.1:CDS
CAATTCTTATGATACGCCGA+GGG 0.635929 5:-67545678 None:intergenic
TGTTTGGAAGGTTTAAGGCG+GGG 0.636113 5:-67544075 None:intergenic
GGCTTTGCAAACCATGCTGG+TGG 0.636879 5:+67545456 MsaT028711.1:CDS
AGCTGTTTGAGGAGTGTGAG+AGG 0.637236 5:+67543885 MsaT028711.1:CDS
GGAAAATTATCCGCTGTGGG+AGG 0.637624 5:+67543471 MsaT028711.1:CDS
CTGAAATCTGGTACCTACGA+GGG 0.639712 5:-67547941 None:intergenic
GCGACTCCAAAACAACAAGA+TGG 0.640491 5:+67548594 MsaT028711.1:CDS
ACTCTCCTACGTTTGAAGGG+AGG 0.642713 5:+67545379 MsaT028711.1:CDS
AGAGAAACCGTAAACGCACA+AGG 0.642907 5:+67545166 MsaT028711.1:CDS
GTTATCCTCCCTTCAAACGT+AGG 0.643744 5:-67545384 None:intergenic
ATAGCAGAAGTGCCCCTCGT+AGG 0.645166 5:+67547928 MsaT028711.1:CDS
CCTTCAAACAGATCATTGTG+CGG 0.646292 5:-67545591 None:intergenic
GATCCACCCCAAATGAGCCA+AGG 0.648744 5:+67544745 MsaT028711.1:CDS
GACAAAACCAATCACCATCA+GGG 0.650816 5:-67545859 None:intergenic
GGTGGATGTGGAATTTGAGA+AGG 0.651302 5:+67544339 MsaT028711.1:CDS
TTAAAGAAAAGATTCCCGAA+TGG 0.651679 5:+67545037 MsaT028711.1:CDS
TGCTGAAAGTGACCTAGTGG+CGG 0.651844 5:+67546733 MsaT028711.1:CDS
ATTGTTTGGAAGGTTTAAGG+CGG 0.652953 5:-67544077 None:intergenic
CTAGAGAAAGGGTTATGCAG+GGG 0.662729 5:-67546809 None:intergenic
TAAACAACTGAGGATCACTC+AGG 0.664286 5:+67543808 MsaT028711.1:CDS
AAATAAAATTGAAGAGACCG+AGG 0.667269 5:+67545910 MsaT028711.1:CDS
ACAATTCTTATGATACGCCG+AGG 0.669642 5:-67545679 None:intergenic
TGAAATCTGGTACCTACGAG+GGG 0.676014 5:-67547940 None:intergenic
TGTTCTGTTTGTAACTACGG+TGG 0.676365 5:+67545630 MsaT028711.1:CDS
ACTGAGAATTGGTTACCAAG+AGG 0.676963 5:+67543845 MsaT028711.1:CDS
TCTGTGGAGAAGAGTGTGAA+CGG 0.679351 5:+67546166 MsaT028711.1:CDS
TTAGATTGTGGCTATCAGCG+AGG 0.682175 5:+67543605 MsaT028711.1:CDS
ACTTGGATGTAGGAGCAAGA+CGG 0.682568 5:+67544489 MsaT028711.1:CDS
TAGAGAGTGAAGTACTTACG+CGG 0.686594 5:+67545217 MsaT028711.1:CDS
GAAGGGAGGATAACTCGCGA+AGG 0.688944 5:+67545393 MsaT028711.1:CDS
GTCACTGTTAGATTGCATGA+GGG 0.696628 5:+67545527 MsaT028711.1:CDS
CAGAATCTATATGGACCTAG+CGG 0.696805 5:+67545435 MsaT028711.1:CDS
CGGTGCTGAAAGTGACCTAG+TGG 0.697890 5:+67546730 MsaT028711.1:CDS
AGATTGCTGTCAATTTGAGG+TGG 0.705765 5:-67544706 None:intergenic
TGATTAGTGTGGCGACTGTG+AGG 0.707183 5:+67547785 MsaT028711.1:CDS
TCACTCATTAGAGAGAAACT+CGG 0.709740 5:+67544861 MsaT028711.1:CDS
TTGGTTACTGATCAAAACTG+TGG 0.709900 5:+67544229 MsaT028711.1:CDS
TATGTCAACTCTGAGACTTG+CGG 0.721346 5:+67546701 MsaT028711.1:CDS
AGAGAGTGAAGTACTTACGC+GGG 0.723801 5:+67545218 MsaT028711.1:CDS
AGGTTGGAGTTAAACAACTG+AGG 0.726333 5:+67543798 MsaT028711.1:CDS
AATTCTTATGATACGCCGAG+GGG 0.728497 5:-67545677 None:intergenic
ACTGAAATCTGGTACCTACG+AGG 0.735704 5:-67547942 None:intergenic
AACGCGTCTTCCTCCCACAG+CGG 0.747933 5:-67543481 None:intergenic
GTGACAGTAGTATTTCACGA+TGG 0.759670 5:-67544842 None:intergenic
TGGACCAATTCTGATCATGG+TGG 0.790764 5:+67545057 MsaT028711.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 67542789 67548737 67542789 ID=MsaG028711
Chr5 mRNA 67542789 67548737 67542789 ID=MsaT028711.1;Parent=MsaG028711
Chr5 exon 67542789 67542881 67542789 ID=MsaT028711.1.exon1;Parent=MsaT028711.1
Chr5 CDS 67542789 67542881 67542789 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67543428 67545713 67543428 ID=MsaT028711.1.exon2;Parent=MsaT028711.1
Chr5 CDS 67543428 67545713 67543428 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67545854 67545971 67545854 ID=MsaT028711.1.exon3;Parent=MsaT028711.1
Chr5 CDS 67545854 67545971 67545854 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67546103 67546188 67546103 ID=MsaT028711.1.exon4;Parent=MsaT028711.1
Chr5 CDS 67546103 67546188 67546103 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67546623 67546867 67546623 ID=MsaT028711.1.exon5;Parent=MsaT028711.1
Chr5 CDS 67546623 67546867 67546623 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67547720 67547806 67547720 ID=MsaT028711.1.exon6;Parent=MsaT028711.1
Chr5 CDS 67547720 67547806 67547720 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67547912 67548011 67547912 ID=MsaT028711.1.exon7;Parent=MsaT028711.1
Chr5 CDS 67547912 67548011 67547912 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67548175 67548382 67548175 ID=MsaT028711.1.exon8;Parent=MsaT028711.1
Chr5 CDS 67548175 67548382 67548175 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Chr5 exon 67548592 67548737 67548592 ID=MsaT028711.1.exon9;Parent=MsaT028711.1
Chr5 CDS 67548592 67548737 67548592 ID=cds.MsaT028711.1;Parent=MsaT028711.1
Gene Sequence

>MsaT028711.1

ATGGAGGTTGATGGTGATAAGAGTGATAATGGAAATCGAAAAAGGAAAAGAGGGTTATTGAAAAGAAAGCTTCTTGTCAATGAAAAAGTTGAGTTGAGGAGTTTGGAAGATGGTTTTCTGGGATCATGGCATCCAGGGAAAATTATCCGCTGTGGGAGGAAGACGCGTTATGTCAGATATGACAATATCCTTGATGATGAAGAATCAGATTATCTTATTGAAGTGGTGGATGTTTCTAATGTTTTGGATGGTGTTAATTCTTCATCTGCTTTAGATTGTGGCTATCAGCGAGGCCTGATTAGACCGTTACCGCCTCCAATTGAGTTACGGATAAAGGATCTTCCATTTGGACAATGTGTTGATGTGAAATATCAGGATGCTTGGTGGGAAGGTGTGATATTCGATCGCTGCGATGGAATGAAGGATAGGAGCATATTCTTCCCAGATTTAGGTGATGAAATGAAGGTTGGAGTTAAACAACTGAGGATCACTCAGGATTGGGATGAATCAACTGAGAATTGGTTACCAAGAGGGAAATGGGTTTTTCTTGAGCTGTTTGAGGAGTGTGAGAGGGTATCCTATGTTGCAGTCTCGGTGAAACAGATTTGGTATGATATAAGGATGAGAAAGGATTTTGCTGAAACCATTAGAGAATGGACTTGTAATGTGAAGGAGTTGTGGAGAGACCTTGTTGTTGAGGTAATTGGTGATTACTATACCCTCACATTAAGCGAAGTTCGCCCCGCCTTAAACCTTCCAAACAATTTACTCGAGGGAGAGTCATTTGAACCAACTGATAATGTTCATTGTGACGCTGCTAATCAAGGCGATGCATTTGGTTCTGACATTGCAATATCTGATAAGCCTGAGGAAAACGCCGACTCCTCAAACTTGTTGGTTACTGATCAAAACTGTGGCAGCACAAGCATCATTCCTTTGGTGGATGGGGAATCTGAGAAGGAAATTCTTTTACATGAAGAGCTTGAATCAGATAAAAATCAAATGGTGGATGTGGAATTTGAGAAGGAAATTCTTTTACATGAAGAGCTTGAATCAGATAAAAATCGAAAACGGAGACGTTCTAAGTCCATAATTTGGAAGCCTTTGGAACTGTCTGAAGTTGAATTCTGTCCTGAAGTTATTGATGAGTATGCACTTGGATGTAGGAGCAAGACGGTTCGGGAGCTTTTGAAGACGAAAGTAAGAAAACATCTTGTTTATCTTGGATGGACAATTGAATGGACCGAAAACAATACTCCTCCACATAGACGATACCGGTACAAGTCTCCGGATAAACTTAATCCAAAGTTTTACACTTCGATTTTTCAAGTTACTAAAATTTTGCAAGAGGATCCTAACATGAACTCAGGACCACCTCAAATTGACAGCAATCTTTCACATCTGCTCTCTGATCCACCCCAAATGAGCCAAGGTTTCAACGTTTGTCCTCCAACCAATGAACCTTCTCCAGTTAAATTTCAAGTTGAACCAGAATTTTGTCCTCTTGCCATCGTGAAATACTACTGTCACTCATTAGAGAGAAACTCGGCTGATAAGAGAAAGTGGAAATTGAAGGCCAAAAAACATCTGTTAGCAGAAGGATGGATGATTGACTACCCAACTGAAAGAAGGAAGACTACTTTGTACAAGTCTCCACAAGATCAGTGTTTAGGCACACTTCAAGGTGCATGCAGACTATACCTTAAAGAAAAGATTCCCGAATGGACCAATTCTGATCATGGTGGTGATGATGATCTACTTTCGAGTGTGTCCCAATTGCTTCAAAAAGAGCCTGAATTACGTACTATAGATGGTTCACCACCAACTGCAAAGAGAAACCGTAAACGCACAAGGACTTCAAAGGCTAGCACACAAAAGGACTTAGAGAGTGAAGTACTTACGCGGGTGCTAAGATCAAGCAAAAGGGTGCAGAAGGTTTTGGGTTCATCATATCAAAAGCCTCAAAATATTCTGTCTTGGTTAATAGATTGCAACATTGTGTTGCCAAAGTACAAGGTCTTTTACTGGGAAACCGAAGGAGGAAACTCTCCTACGTTTGAAGGGAGGATAACTCGCGAAGGAATTAGGTGCACCTGTTGTCAGAATCTATATGGACCTAGCGGCTTTGCAAACCATGCTGGTGGAAGCAGTAACTGCAGACCTTCAGCTTGTATATTTTTGAAGGATGGTAGGTCACTGTTAGATTGCATGAGGGAAGTAATGCAAGATCACAGGACAAGAGAAATCACGGAGAAACCGCACAATGATCTGTTTGAAGGTGAAAATGACAACATTTGTTCTGTTTGTAACTACGGTGGTGAACTCATTTTATGCGACCAATGCCCCTCGGCGTATCATAAGAATTGTCTTAATTTGGAGGGCATCCCTGATGGTGATTGGTTTTGTCCATCATGCCGTTGCGGGATTTGCAGCCAAAATAAAATTGAAGAGACCGAGGATGGACACTTTCTTACTTGCATTCAGTGTGAACATAAATATCATGTTGAGTGCCTCAGAAATGAAGAAAAAGATGATTCGAGACGATATATGAAAAATTGGCTCTGTGGAGAAGAGTGTGAACGGGTCTATACGGGGCTCCAAAATCTATTAGGAAAGCCGGTTTTAGTGGGGGCGGACAATCTAACTTGGACATTGGTGAAGTATGTCAACTCTGAGACTTGCGGTGTTGGCGGTGCTGAAAGTGACCTAGTGGCGGAGAATTACAGCAAACTGAGTGTTGCACTCTCGGTGATGCATGAATGTTTTGAGCCCCTGCATAACCCTTTCTCTAGCAGAGATATCGTTGAAGACGTTATATTCAACCAAAGGTCTGAGCTCAATCGACTAAACTTCCAGGGTTTCTATACTGTACTTCTTGAGAGAAATGAAGAATTGATTAGTGTGGCGACTGTGAGGATATTTGGAGAGAAGATAGCAGAAGTGCCCCTCGTAGGTACCAGATTTCAGTACAGACGACTTGGAATGTGTCGCATTTTAATGGATGAGCTCGAAAAGAAGCTAAAGCAACTGGGAGTGGAAAGACTAGTCTTGCCTGCTGTTCCCGGTGTGCTAGACACATGGACGAATTCTTTTGGTTTTGAGCAGATGACGAATTTTGAGAGGTCAAAGTTTCTGGACTATTCTTTCCTAGACTTTCAAGGAACTGTCATGTGTCAGAAACTGTTGACAAGATTTCCATCTCCGGAATCGGTTGTAACAAGAGACGCGACTCCAAAACAACAAGATGGTAACTTCTCTGTTAAATGTAAAATTAATTTTGAAAAGGCTAGTCCAGTATCTGAAGTGGATCAAGCAGAAGGGATTAGCAAAAGAGGAATGATGGATCTACAAGTTTGGAGACCTTCCTAA
Protein sequence

>MsaT028711.1

MEVDGDKSDNGNRKRKRGLLKRKLLVNEKVELRSLEDGFLGSWHPGKIIRCGRKTRYVRYDNILDDEESDYLIEVVDVSNVLDGVNSSSALDCGYQRGLIRPLPPPIELRIKDLPFGQCVDVKYQDAWWEGVIFDRCDGMKDRSIFFPDLGDEMKVGVKQLRITQDWDESTENWLPRGKWVFLELFEECERVSYVAVSVKQIWYDIRMRKDFAETIREWTCNVKELWRDLVVEVIGDYYTLTLSEVRPALNLPNNLLEGESFEPTDNVHCDAANQGDAFGSDIAISDKPEENADSSNLLVTDQNCGSTSIIPLVDGESEKEILLHEELESDKNQMVDVEFEKEILLHEELESDKNRKRRRSKSIIWKPLELSEVEFCPEVIDEYALGCRSKTVRELLKTKVRKHLVYLGWTIEWTENNTPPHRRYRYKSPDKLNPKFYTSIFQVTKILQEDPNMNSGPPQIDSNLSHLLSDPPQMSQGFNVCPPTNEPSPVKFQVEPEFCPLAIVKYYCHSLERNSADKRKWKLKAKKHLLAEGWMIDYPTERRKTTLYKSPQDQCLGTLQGACRLYLKEKIPEWTNSDHGGDDDLLSSVSQLLQKEPELRTIDGSPPTAKRNRKRTRTSKASTQKDLESEVLTRVLRSSKRVQKVLGSSYQKPQNILSWLIDCNIVLPKYKVFYWETEGGNSPTFEGRITREGIRCTCCQNLYGPSGFANHAGGSSNCRPSACIFLKDGRSLLDCMREVMQDHRTREITEKPHNDLFEGENDNICSVCNYGGELILCDQCPSAYHKNCLNLEGIPDGDWFCPSCRCGICSQNKIEETEDGHFLTCIQCEHKYHVECLRNEEKDDSRRYMKNWLCGEECERVYTGLQNLLGKPVLVGADNLTWTLVKYVNSETCGVGGAESDLVAENYSKLSVALSVMHECFEPLHNPFSSRDIVEDVIFNQRSELNRLNFQGFYTVLLERNEELISVATVRIFGEKIAEVPLVGTRFQYRRLGMCRILMDELEKKLKQLGVERLVLPAVPGVLDTWTNSFGFEQMTNFERSKFLDYSFLDFQGTVMCQKLLTRFPSPESVVTRDATPKQQDGNFSVKCKINFEKASPVSEVDQAEGISKRGMMDLQVWRPS*