Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028736 | GAU29750.1 | 77.193 | 57 | 13 | 0 | 1 | 57 | 1 | 57 | 3.01e-24 | 104 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028736 | tr|A0A2Z6N136|A0A2Z6N136_TRISU | 77.193 | 57 | 13 | 0 | 1 | 57 | 1 | 57 | 1.44e-24 | 104 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG028736 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG028736 | MtrunA17_Chr7g0258861 | 58.824 | 51 | 21 | 0 | 7 | 57 | 10 | 60 | 1.71e-17 | 74.3 |
| MsaG028736 | MtrunA17_Chr7g0238821 | 59.574 | 47 | 19 | 0 | 11 | 57 | 1 | 47 | 3.07e-15 | 64.7 |
| MsaG028736 | MtrunA17_Chr2g0324011 | 56.863 | 51 | 22 | 0 | 7 | 57 | 10 | 60 | 4.36e-15 | 68.2 |
| MsaG028736 | MtrunA17_Chr6g0458391 | 50.000 | 52 | 25 | 1 | 7 | 57 | 225 | 276 | 9.18e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATGAAGCTTGGGAATTT+TGG | 0.196222 | 5:-67901979 | MsaT028736.1:CDS |
| CTTGGTTATGGAAAAGAAAT+TGG | 0.196698 | 5:-67901956 | MsaT028736.1:CDS |
| TATGGAAAAGAAATTGGTTT+TGG | 0.264632 | 5:-67901950 | MsaT028736.1:CDS |
| AGGCAGGTTTGTTTGTTGTA+AGG | 0.405014 | 5:-67901879 | MsaT028736.1:CDS |
| TACTACACCCGCTGTTGGTA+TGG | 0.424152 | 5:-67902014 | MsaT028736.1:CDS |
| TTGACAGTGTAGATGAAGCT+TGG | 0.458332 | 5:-67901988 | MsaT028736.1:CDS |
| TGACAGTGTAGATGAAGCTT+GGG | 0.472271 | 5:-67901987 | MsaT028736.1:CDS |
| AGGAGGTGTCATGGAGGTGC+AGG | 0.499127 | 5:-67901859 | MsaT028736.1:CDS |
| AAAGATGGCTCAATCAGATC+AGG | 0.507448 | 5:-67901899 | MsaT028736.1:CDS |
| CAGGTTTGTTTGTTGTAAGG+AGG | 0.511221 | 5:-67901876 | MsaT028736.1:CDS |
| AGGAGAAGTGGAAGCAAGAA+GGG | 0.518981 | 5:-67901839 | MsaT028736.1:CDS |
| CAGGAGAAGTGGAAGCAAGA+AGG | 0.522651 | 5:-67901840 | MsaT028736.1:CDS |
| TTTGTTGTAAGGAGGTGTCA+TGG | 0.543167 | 5:-67901868 | MsaT028736.1:CDS |
| TCATGGAGGTGCAGGAGAAG+TGG | 0.551987 | 5:-67901851 | MsaT028736.1:CDS |
| GGGAGAATCTTCAAAAGTGA+GGG | 0.573414 | 5:-67901819 | MsaT028736.1:CDS |
| GTCAAATTCCATACCAACAG+CGG | 0.581235 | 5:+67902006 | None:intergenic |
| TACTTTAACAGAAAGAAAGA+TGG | 0.586825 | 5:-67901914 | MsaT028736.1:CDS |
| AAAATTACTACACCCGCTGT+TGG | 0.596539 | 5:-67902019 | MsaT028736.1:CDS |
| TCAAATTCCATACCAACAGC+GGG | 0.615051 | 5:+67902007 | None:intergenic |
| AGGGAGAATCTTCAAAAGTG+AGG | 0.624972 | 5:-67901820 | MsaT028736.1:CDS |
| ATGGCTCAATCAGATCAGGC+AGG | 0.631878 | 5:-67901895 | MsaT028736.1:CDS |
| GGAGAATCTTCAAAAGTGAG+GGG | 0.698850 | 5:-67901818 | MsaT028736.1:CDS |
| GTTGTAAGGAGGTGTCATGG+AGG | 0.709644 | 5:-67901865 | MsaT028736.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 67901814 | 67902047 | 67901814 | ID=MsaG028736 |
| Chr5 | mRNA | 67901814 | 67902047 | 67901814 | ID=MsaT028736.1;Parent=MsaG028736 |
| Chr5 | exon | 67901814 | 67902047 | 67901814 | ID=MsaT028736.1.exon1;Parent=MsaT028736.1 |
| Chr5 | CDS | 67901814 | 67902047 | 67901814 | ID=cds.MsaT028736.1;Parent=MsaT028736.1 |
| Gene Sequence |
| Protein sequence |
