AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028755

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028755 MtrunA17_Chr5g0436501 99.262 271 2 0 1 271 1 271 0.0 566
MsaG028755 MtrunA17_Chr7g0241761 62.150 214 72 2 3 209 11 222 6.61e-90 269
MsaG028755 MtrunA17_Chr5g0429521 61.386 202 71 3 2 198 4 203 6.88e-85 256
MsaG028755 MtrunA17_Chr3g0104071 64.796 196 56 4 16 200 27 220 7.46e-83 251
MsaG028755 MtrunA17_Chr1g0162081 51.832 191 76 4 25 202 10 197 1.93e-56 183
MsaG028755 MtrunA17_Chr1g0210471 49.733 187 80 4 25 204 22 201 4.93e-55 177
MsaG028755 MtrunA17_Chr4g0062741 42.381 210 81 5 27 201 8 212 2.17e-47 160
MsaG028755 MtrunA17_Chr8g0365011 43.229 192 79 4 25 202 6 181 1.01e-38 137
MsaG028755 MtrunA17_Chr2g0331031 61.111 90 33 1 120 209 104 191 1.42e-29 114
MsaG028755 MtrunA17_Chr6g0488471 69.863 73 20 1 119 191 91 161 7.36e-29 111
MsaG028755 MtrunA17_Chr4g0055931 64.198 81 27 1 120 200 90 168 6.18e-28 109
MsaG028755 MtrunA17_Chr8g0389241 60.714 84 30 1 116 199 96 176 6.00e-27 105
MsaG028755 MtrunA17_Chr5g0415191 60.000 90 34 1 112 201 89 176 7.00e-26 103
MsaG028755 MtrunA17_Chr4g0059701 71.186 59 17 0 113 171 72 130 1.01e-24 100
MsaG028755 MtrunA17_Chr5g0409841 49.524 105 49 1 92 192 55 159 7.77e-24 96.3
MsaG028755 MtrunA17_Chr8g0364741 40.984 122 66 2 25 142 8 127 1.21e-23 93.2
MsaG028755 MtrunA17_Chr5g0440741 53.933 89 39 2 105 192 81 168 4.83e-23 95.5
MsaG028755 MtrunA17_Chr3g0135671 51.429 105 44 4 90 191 58 158 9.07e-23 93.6
MsaG028755 MtrunA17_Chr5g0440731 58.904 73 29 1 121 192 101 173 9.48e-23 94.7
MsaG028755 MtrunA17_Chr1g0155181 55.882 68 30 0 22 89 2 69 3.75e-21 85.1
MsaG028755 MtrunA17_Chr3g0092621 57.971 69 27 1 125 193 84 150 1.68e-20 85.9
MsaG028755 MtrunA17_Chr3g0141601 53.846 65 30 0 25 89 5 69 1.23e-19 81.3
MsaG028755 MtrunA17_Chr6g0449111 52.941 68 32 0 22 89 5 72 8.18e-19 78.6
MsaG028755 MtrunA17_Chr5g0409831 58.904 73 29 1 121 192 73 145 1.17e-18 82.4
MsaG028755 MtrunA17_Chr5g0432921 33.000 200 119 7 26 218 20 211 3.28e-18 81.3
MsaG028755 MtrunA17_Chr7g0255841 52.941 68 32 0 30 97 15 82 4.22e-18 77.4
MsaG028755 MtrunA17_Chr8g0364731 60.294 68 27 0 135 202 11 78 6.83e-18 79.3
MsaG028755 MtrunA17_Chr3g0113121 40.764 157 79 7 48 196 21 171 9.21e-18 79.7
MsaG028755 MtrunA17_Chr6g0452791 57.627 59 25 0 125 183 80 138 9.76e-18 78.6
MsaG028755 MtrunA17_Chr3g0144631 52.381 63 30 0 27 89 16 78 1.41e-17 75.5
MsaG028755 MtrunA17_Chr5g0404101 45.333 75 41 0 15 89 7 81 3.93e-17 74.7
MsaG028755 MtrunA17_Chr3g0081631 47.945 73 37 1 18 89 1 73 1.13e-16 73.6
MsaG028755 MtrunA17_Chr5g0432941 30.000 200 124 5 26 218 21 211 1.91e-16 76.3
MsaG028755 MtrunA17_Chr8g0373131 49.231 65 32 1 25 89 3 66 2.09e-15 69.3
MsaG028755 MtrunA17_Chr8g0373111 49.231 65 32 1 25 89 3 66 2.19e-15 69.3
MsaG028755 MtrunA17_Chr8g0373121 49.231 65 32 1 25 89 3 66 2.19e-15 69.3
MsaG028755 MtrunA17_Chr8g0373101 50.820 61 29 1 27 87 5 64 4.59e-14 65.9
MsaG028755 MtrunA17_Chr5g0404301 50.820 61 29 1 27 87 4 63 2.16e-13 63.9
MsaG028755 MtrunA17_Chr1g0192061 55.714 70 29 2 127 195 63 131 2.73e-13 66.6
MsaG028755 MtrunA17_Chr3g0141641 38.806 67 41 0 27 93 3 69 3.72e-13 63.9
MsaG028755 MtrunA17_Chr8g0370121 50.000 50 25 0 122 171 97 146 7.47e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028755 AT2G38090.1 59.716 211 69 2 3 199 5 213 4.50e-86 259
MsaG028755 AT3G11280.2 66.138 189 55 3 27 212 33 215 1.59e-81 246
MsaG028755 AT3G11280.1 66.138 189 55 3 27 212 33 215 1.59e-81 246
MsaG028755 AT5G05790.2 56.828 227 88 6 3 222 4 227 1.87e-79 241
MsaG028755 AT5G05790.1 56.828 227 88 6 3 222 4 227 1.87e-79 241
MsaG028755 AT5G58900.1 58.019 212 70 4 16 210 15 224 4.49e-77 236
MsaG028755 AT5G01200.1 54.067 209 73 6 6 196 12 215 7.87e-67 209
MsaG028755 AT5G08520.1 51.087 184 80 2 27 202 12 193 7.67e-54 176
MsaG028755 AT1G49010.1 45.050 202 81 4 21 194 2 201 1.54e-49 166
MsaG028755 AT5G04760.1 43.333 210 89 7 25 231 4 186 2.94e-46 154
MsaG028755 AT5G23650.1 42.654 211 89 5 20 215 6 199 3.92e-44 152
MsaG028755 AT3G10580.2 38.164 207 98 7 27 231 8 186 3.32e-35 127
MsaG028755 AT3G10580.1 38.164 207 98 7 27 231 8 186 6.51e-35 127
MsaG028755 AT4G09450.1 42.515 167 80 4 26 192 6 156 6.49e-32 117
MsaG028755 AT5G56840.1 63.415 82 29 1 120 201 86 166 1.24e-27 107
MsaG028755 AT5G47390.1 66.250 80 25 1 112 191 83 160 1.40e-27 109
MsaG028755 AT5G61620.1 52.941 102 46 1 91 192 74 173 7.30e-27 106
MsaG028755 AT1G19000.2 51.327 113 48 3 112 219 89 199 3.66e-25 101
MsaG028755 AT1G19000.1 51.327 113 48 3 112 219 89 199 3.66e-25 101
MsaG028755 AT1G70000.1 72.131 61 17 0 112 172 85 145 6.55e-25 100
MsaG028755 AT1G70000.2 72.131 61 17 0 112 172 85 145 6.55e-25 100
MsaG028755 AT3G16350.1 55.238 105 42 2 116 217 125 227 6.58e-25 102
MsaG028755 AT3G10590.1 40.196 204 96 12 27 216 7 198 6.47e-23 93.6
MsaG028755 AT1G74840.2 75.000 52 13 0 120 171 91 142 6.11e-22 92.0
MsaG028755 AT1G74840.1 75.000 52 13 0 120 171 91 142 8.73e-22 92.0
MsaG028755 AT1G75250.2 55.556 63 28 0 27 89 12 74 6.45e-18 77.4
MsaG028755 AT1G75250.1 55.556 63 28 0 27 89 12 74 7.93e-18 77.8
MsaG028755 AT4G39250.1 48.485 66 34 0 27 92 14 79 1.49e-17 76.3
MsaG028755 AT2G21650.1 47.761 67 35 0 27 93 14 80 4.00e-16 72.8
MsaG028755 AT2G18328.1 54.098 61 27 1 29 89 14 73 4.77e-14 66.2
MsaG028755 AT1G19510.1 51.724 58 28 0 32 89 17 74 4.05e-13 64.3
MsaG028755 AT3G10595.1 29.054 148 81 5 24 171 3 126 3.57e-12 63.9

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCCTATGCAGCAGAAAA+TGG 0.222210 5:+68084031 MsaT028755.1:CDS
TATGCTAAGCTGAAAGTTTC+GGG 0.251091 5:+68084128 MsaT028755.1:CDS
CTGATGGGACTTCTAAAGTA+TGG 0.266546 5:+68083801 MsaT028755.1:CDS
ACCAGGAATCGGAACATTTC+CGG 0.268577 5:-68082836 None:intergenic
GCCGGAAATGTTCCGATTCC+TGG 0.317971 5:+68082835 MsaT028755.1:CDS
TTATGCTAAGCTGAAAGTTT+CGG 0.326332 5:+68084127 MsaT028755.1:CDS
CTTCTCTACTCCATTCTGTT+TGG 0.332735 5:-68082663 None:intergenic
AGGAGACACATTGACTAATA+AGG 0.335368 5:-68084015 None:intergenic
TGATGTGATTAAGCAGTATA+GGG 0.335951 5:+68082783 MsaT028755.1:CDS
ACATAAAATAAGGAGAATAA+AGG 0.337256 5:-68082609 None:intergenic
TGTGATTTAAGGTATGGATT+TGG 0.345223 5:+68082582 None:intergenic
AGAAGGCATGTGACTGTTAG+AGG 0.384719 5:+68082919 MsaT028755.1:CDS
TTGATGTGATTAAGCAGTAT+AGG 0.386148 5:+68082782 MsaT028755.1:CDS
AAGATTGCTGAGATGATTCC+TGG 0.391655 5:+68082748 MsaT028755.1:CDS
TTTGTATCCAACATAAAATA+AGG 0.405866 5:-68082619 None:intergenic
ATAAGGCAAAAGGTTTCTTC+TGG 0.415949 5:+68083909 MsaT028755.1:CDS
GCAATCTTAAGCCATCTATC+TGG 0.417904 5:-68082733 None:intergenic
TAAAGTATGGCAAAGGCGAC+TGG 0.439380 5:+68083814 MsaT028755.1:CDS
CATAAAATAAGGAGAATAAA+GGG 0.445828 5:-68082608 None:intergenic
GATTAAGCAGTATAGGGAAT+TGG 0.449715 5:+68082789 MsaT028755.1:CDS
GCATGACTTGCTACTTGTGT+TGG 0.455619 5:-68083873 None:intergenic
TGCTCTTGCTATATATGATA+AGG 0.457525 5:+68082705 MsaT028755.1:CDS
ATAAGGATACACCAGATAGA+TGG 0.475384 5:+68082722 MsaT028755.1:CDS
ATGCACAAAAGTACTACATA+AGG 0.480834 5:+68083892 MsaT028755.1:CDS
AGAGGAAGAAAGGTGTTCCT+TGG 0.489408 5:+68082953 MsaT028755.1:CDS
AAAGTACTACATAAGGCAAA+AGG 0.510073 5:+68083899 MsaT028755.1:CDS
AATAAGGACTTGTTATCTGA+TGG 0.512003 5:-68083999 None:intergenic
GAGGCAAGATAACCAGGAAT+CGG 0.520347 5:-68082847 None:intergenic
TGATGGATCACCACAAGGGC+AGG 0.524353 5:+68084076 MsaT028755.1:CDS
TCAGCTTAGCATAAGCCTCA+TGG 0.535139 5:-68084118 None:intergenic
TCTCCTATGCAGCAGAAAAT+GGG 0.535185 5:+68084032 MsaT028755.1:CDS
AGTACTAGCCACTACAATGA+TGG 0.540673 5:+68084059 MsaT028755.1:CDS
TCTGATCATGAGAGGAAGAA+AGG 0.544690 5:+68082943 MsaT028755.1:CDS
AAAGAAGAGGCAAGATAACC+AGG 0.552310 5:-68082853 None:intergenic
AAGAGAGTTACCAAACAGAA+TGG 0.556624 5:+68082653 MsaT028755.1:CDS
AGGCAAAAGGTTTCTTCTGG+AGG 0.582472 5:+68083912 MsaT028755.1:CDS
ACTATGATGGAAACAGAAGA+AGG 0.582942 5:+68082902 MsaT028755.1:CDS
TCATAGAGATCCTGCCCTTG+TGG 0.585420 5:-68084086 None:intergenic
CTTGGACTGAAGAGGAACAT+AGG 0.585629 5:+68082971 MsaT028755.1:CDS
CTATGTTCCTCTTCAGTCCA+AGG 0.586053 5:-68082970 None:intergenic
CTATGCAGCAGAAAATGGGT+TGG 0.587490 5:+68084036 MsaT028755.1:CDS
AGGTGTTCCTTGGACTGAAG+AGG 0.592742 5:+68082963 MsaT028755.1:CDS
GTTGTGATATCATGAATGCT+TGG 0.600782 5:-68083951 None:intergenic
CTATGACTGCTCTTTCCATG+AGG 0.601527 5:+68084103 MsaT028755.1:CDS
GCTTCTAGAGACTTCAACAA+AGG 0.612158 5:+68084161 MsaT028755.1:CDS
GATGTGAGTGAAATTGAGGC+CGG 0.613302 5:+68082817 MsaT028755.1:CDS
TACAATGATGGATCACCACA+AGG 0.622159 5:+68084071 MsaT028755.1:CDS
AACTTCAAAGGTGAAAGAAG+AGG 0.626616 5:-68082866 None:intergenic
TTAGAGGTTCTGATCATGAG+AGG 0.633143 5:+68082935 MsaT028755.1:CDS
GTGGTGATCCATCATTGTAG+TGG 0.633599 5:-68084067 None:intergenic
AGAAGATGTGAGTGAAATTG+AGG 0.635385 5:+68082813 MsaT028755.1:CDS
GGACTTCTAAAGTATGGCAA+AGG 0.636983 5:+68083807 MsaT028755.1:CDS
ACAATGATGGATCACCACAA+GGG 0.720384 5:+68084072 MsaT028755.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 68082595 68084211 68082595 ID=MsaG028755
Chr5 mRNA 68082595 68084211 68082595 ID=MsaT028755.1;Parent=MsaG028755
Chr5 exon 68082595 68082992 68082595 ID=MsaT028755.1.exon1;Parent=MsaT028755.1
Chr5 CDS 68082595 68082992 68082595 ID=cds.MsaT028755.1;Parent=MsaT028755.1
Chr5 exon 68083794 68084211 68083794 ID=MsaT028755.1.exon2;Parent=MsaT028755.1
Chr5 CDS 68083794 68084211 68083794 ID=cds.MsaT028755.1;Parent=MsaT028755.1
Gene Sequence

>MsaT028755.1

ATGGATTTGGAAACCCTTTATTCTCCTTATTTTATGTTGGATACAAATTGGCTTGTTGAAGAGAGTTACCAAACAGAATGGAGTAGAGAAGATAACAAGAAATTTGAAAGTGCTCTTGCTATATATGATAAGGATACACCAGATAGATGGCTTAAGATTGCTGAGATGATTCCTGGTAAGACTGTTTTTGATGTGATTAAGCAGTATAGGGAATTGGTAGAAGATGTGAGTGAAATTGAGGCCGGAAATGTTCCGATTCCTGGTTATCTTGCCTCTTCTTTCACCTTTGAAGTTGTCGAAAAACAAAACTATGATGGAAACAGAAGAAGGCATGTGACTGTTAGAGGTTCTGATCATGAGAGGAAGAAAGGTGTTCCTTGGACTGAAGAGGAACATAGACGTTTTCTGATGGGACTTCTAAAGTATGGCAAAGGCGACTGGAGAAACATCTCGCGCAATTTCGTCGTGACAAAGACTCCAACACAAGTAGCAAGTCATGCACAAAAGTACTACATAAGGCAAAAGGTTTCTTCTGGAGGCAAAGATAAAAGAAGACCAAGCATTCATGATATCACAACTGTTACTCTCACAGAAACTTCTTCTCCATCAGATAACAAGTCCTTATTAGTCAATGTGTCTCCTATGCAGCAGAAAATGGGTTGGAGTACTAGCCACTACAATGATGGATCACCACAAGGGCAGGATCTCTATGACTGCTCTTTCCATGAGGCTTATGCTAAGCTGAAAGTTTCGGGTTTCGCGACAGCTTCTAGAGACTTCAACAAAGGAGCTGTTTTTGGTATTCATGCACTGTAA
Protein sequence

>MsaT028755.1

MDLETLYSPYFMLDTNWLVEESYQTEWSREDNKKFESALAIYDKDTPDRWLKIAEMIPGKTVFDVIKQYRELVEDVSEIEAGNVPIPGYLASSFTFEVVEKQNYDGNRRRHVTVRGSDHERKKGVPWTEEEHRRFLMGLLKYGKGDWRNISRNFVVTKTPTQVASHAQKYYIRQKVSSGGKDKRRPSIHDITTVTLTETSSPSDNKSLLVNVSPMQQKMGWSTSHYNDGSPQGQDLYDCSFHEAYAKLKVSGFATASRDFNKGAVFGIHAL*