AlfalfaGEDB | MsaG028757

M. sativa ssp. caerulea / MsaG028757

KEGG: -
GO: -
PFAM: PF08698.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028757	XP_003616568.1	98.524	271	4	0	188	458	1	271	0.0	561

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028757	sp\|Q8S9H7\|DIV_ANTMA	62.000	200	64	5	204	393	15	212	6.35e-77	245

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028757	tr\|G7KBX0\|G7KBX0_MEDTR	98.524	271	4	0	188	458	1	271	0.0	561

TFs/TRs:

Gene ID	Type	Classification
MsaG028757	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000107	MsaG028757	0.805866	1.837399e-49	2.842442e-47
MsaG000609	MsaG028757	0.808875	4.259445e-50	7.079276e-48
MsaG001531	MsaG028757	0.803205	6.556159e-49	9.534679e-47
MsaG002978	MsaG028757	0.816752	8.170988e-52	1.651531e-49
MsaG003174	MsaG028757	0.805529	2.161150e-49	3.316854e-47
MsaG003768	MsaG028757	0.820409	1.221142e-52	2.714975e-50
MsaG004556	MsaG028757	0.821976	5.335396e-53	1.236529e-50
MsaG005189	MsaG028757	0.826789	3.985704e-54	1.053572e-51
MsaG005274	MsaG028757	0.813282	4.771845e-51	8.834572e-49
MsaG006311	MsaG028757	0.800723	2.109544e-48	2.899232e-46
MsaG006529	MsaG028757	0.842470	4.719081e-58	1.992271e-55
MsaG007040	MsaG028757	0.811523	1.151128e-50	2.040293e-48
MsaG007501	MsaG028757	0.800514	2.326527e-48	3.182264e-46
MsaG007828	MsaG028757	0.843646	2.301867e-58	1.009111e-55
MsaG008407	MsaG028757	0.816533	9.143044e-52	1.837587e-49
MsaG009015	MsaG028757	0.813627	4.010734e-51	7.490098e-49
MsaG009061	MsaG028757	0.801855	1.240438e-48	1.749046e-46
MsaG010079	MsaG028757	0.812768	6.178826e-51	1.129358e-48
MsaG010104	MsaG028757	0.814743	2.280343e-51	4.379539e-49
MsaG010278	MsaG028757	0.805894	1.812718e-49	2.806102e-47
MsaG010313	MsaG028757	0.856426	6.298677e-62	4.285999e-59
MsaG010365	MsaG028757	0.801807	1.268920e-48	1.787238e-46
MsaG010768	MsaG028757	0.801686	1.342914e-48	1.886251e-46
MsaG011298	MsaG028757	0.801814	1.264744e-48	1.781629e-46
MsaG014153	MsaG028757	0.810399	2.010798e-50	3.467521e-48
MsaG014247	MsaG028757	0.813130	5.151237e-51	9.500907e-49
MsaG015018	MsaG028757	0.849532	5.785134e-60	3.086056e-57
MsaG015262	MsaG028757	0.866033	7.682669e-65	7.564465e-62
MsaG017286	MsaG028757	0.802041	1.136210e-48	1.608832e-46
MsaG017518	MsaG028757	0.820464	1.186379e-52	2.641531e-50
MsaG017533	MsaG028757	0.826769	4.029608e-54	1.064592e-51
MsaG017620	MsaG028757	0.822243	4.629802e-53	1.080699e-50
MsaG017719	MsaG028757	0.820327	1.274689e-52	2.828027e-50
MsaG017778	MsaG028757	0.823809	2.005651e-53	4.884275e-51
MsaG017781	MsaG028757	0.824575	1.328033e-53	3.302028e-51
MsaG017875	MsaG028757	0.806350	1.454763e-49	2.276447e-47
MsaG018447	MsaG028757	0.819886	1.606823e-52	3.523197e-50
MsaG018489	MsaG028757	0.861500	1.939308e-63	1.596371e-60
MsaG018770	MsaG028757	0.831537	2.848178e-55	8.615664e-53
MsaG018786	MsaG028757	0.861605	1.801014e-63	1.488603e-60
MsaG018793	MsaG028757	0.822500	4.038487e-53	9.492071e-51
MsaG019308	MsaG028757	0.807176	9.755876e-50	1.556605e-47
MsaG019564	MsaG028757	0.841538	8.297928e-58	3.400271e-55
MsaG019935	MsaG028757	0.832971	1.262496e-55	3.982005e-53
MsaG019985	MsaG028757	0.845915	5.667314e-59	2.676477e-56
MsaG020313	MsaG028757	0.813485	4.308360e-51	8.017261e-49
MsaG020407	MsaG028757	0.817782	4.803731e-52	9.970194e-50
MsaG020420	MsaG028757	0.825314	8.901406e-54	2.258570e-51
MsaG020467	MsaG028757	0.813539	4.192988e-51	7.813189e-49
MsaG021200	MsaG028757	0.807738	7.423258e-50	1.200437e-47
MsaG021488	MsaG028757	0.804931	2.879253e-49	4.358110e-47
MsaG021775	MsaG028757	0.821185	8.109952e-53	1.840553e-50
MsaG022345	MsaG028757	0.821675	6.260450e-53	1.439320e-50
MsaG022515	MsaG028757	0.831187	3.469540e-55	1.038818e-52
MsaG022533	MsaG028757	0.807262	9.354345e-50	1.495563e-47
MsaG023623	MsaG028757	0.804931	2.879253e-49	4.358110e-47
MsaG024114	MsaG028757	0.834646	4.835791e-56	1.602808e-53
MsaG025289	MsaG028757	0.827289	3.030334e-54	8.123281e-52
MsaG026022	MsaG028757	0.811456	1.189836e-50	2.105505e-48
MsaG026478	MsaG028757	0.820091	1.443156e-52	3.181718e-50
MsaG026849	MsaG028757	0.835820	2.452048e-56	8.417591e-54
MsaG026831	MsaG028757	0.808320	5.587943e-50	9.163616e-48
MsaG026985	MsaG028757	0.808160	6.040359e-50	9.867827e-48
MsaG028109	MsaG028757	0.858089	2.042848e-62	1.477931e-59
MsaG028757	MsaG029022	0.818209	3.850927e-52	8.081319e-50
MsaG028757	MsaG029103	0.804078	4.328943e-49	6.424350e-47
MsaG028757	MsaG029349	0.828952	1.209882e-54	3.398058e-52
MsaG028757	MsaG029402	0.806246	1.530238e-49	2.388658e-47
MsaG028757	MsaG029413	0.857172	3.807791e-62	2.662293e-59
MsaG028757	MsaG029422	0.801404	1.532985e-48	2.139491e-46
MsaG028757	MsaG029713	0.836301	1.853597e-56	6.456188e-54
MsaG028757	MsaG029802	0.820452	1.193554e-52	2.656771e-50
MsaG028757	MsaG030827	0.803762	5.032004e-49	7.412920e-47
MsaG028757	MsaG031153	0.812989	5.527988e-51	1.016018e-48
MsaG028757	MsaG032272	0.814348	2.784858e-51	5.295886e-49
MsaG028757	MsaG032221	0.810940	1.537749e-50	2.686933e-48
MsaG028757	MsaG033593	0.837533	9.014107e-57	3.259143e-54
MsaG028757	MsaG033965	0.803431	5.887635e-49	8.607221e-47
MsaG028757	MsaG034854	0.885521	1.632806e-71	3.835761e-68
MsaG028757	MsaG034977	0.815166	1.838851e-51	3.569503e-49
MsaG028757	MsaG034946	0.809013	3.980785e-50	6.638031e-48
MsaG028757	MsaG039734	0.809774	2.737374e-50	4.649277e-48
MsaG028757	MsaG041336	0.815572	1.494866e-51	2.931831e-49
MsaG028757	MsaG041819	0.830052	6.556666e-55	1.900400e-52
MsaG028757	MsaG041974	0.803901	4.708158e-49	6.958175e-47
MsaG028757	MsaG042054	0.810586	1.832600e-50	3.174597e-48
MsaG028757	MsaG042579	0.880834	8.381858e-70	1.573186e-66
MsaG028757	MsaG042717	0.818942	2.630585e-52	5.626722e-50
MsaG028757	MsaG043788	0.807589	7.978453e-50	1.285600e-47
MsaG028757	MsaG044278	0.842881	3.673079e-58	1.571232e-55
MsaG028757	MsaG045238	0.807973	6.619910e-50	1.076599e-47
MsaG028757	MsaG045371	0.848709	9.773089e-60	5.068583e-57
MsaG028757	MsaG045372	0.804216	4.053180e-49	6.034261e-47
MsaG028757	MsaG045373	0.802431	9.453649e-49	1.350632e-46
MsaG028757	MsaG045374	0.839089	3.596319e-57	1.364479e-54
MsaG028757	MsaG045568	0.832715	1.461229e-55	4.574447e-53
MsaG028757	MsaG045767	0.809785	2.722191e-50	4.624741e-48
MsaG028757	MsaG046473	0.836040	2.158090e-56	7.457394e-54
MsaG028757	MsaG046498	0.804286	3.919600e-49	5.844912e-47
MsaG028757	MsaG046507	0.819379	2.094497e-52	4.531797e-50
MsaG028757	MsaG046816	0.807740	7.414028e-50	1.199018e-47
MsaG028757	MsaG001564	0.804374	3.758093e-49	5.615437e-47
MsaG028757	MsaG001950	0.814080	3.189817e-51	6.025161e-49
MsaG028757	MsaG010134	0.824794	1.179858e-53	2.951177e-51
MsaG028757	MsaG009144	0.826640	4.324280e-54	1.138340e-51
MsaG028757	MsaG013252	0.811138	1.393755e-50	2.447166e-48
MsaG028757	MsaG021388	0.811423	1.209669e-50	2.138850e-48
MsaG028757	MsaG017935	0.815952	1.231476e-51	2.438676e-49
MsaG028757	MsaG028121	0.821635	6.393182e-53	1.468302e-50
MsaG028757	MsaG026816	0.869679	5.247970e-66	6.000539e-63
MsaG028757	MsaG027199	0.870437	2.973757e-66	3.509516e-63
MsaG028757	MsaG032486	0.811663	1.073516e-50	1.909300e-48
MsaG028757	MsaG030007	0.818249	3.772641e-52	7.924903e-50
MsaG028757	MsaG034262	0.823022	3.056106e-53	7.285271e-51
MsaG028757	MsaG037798	0.824544	1.350177e-53	3.354366e-51
MsaG028757	MsaG036471	0.801562	1.423695e-48	1.994028e-46
MsaG028757	MsaG037545	0.802378	9.695023e-49	1.383447e-46
MsaG028757	MsaG035795	0.832105	2.065160e-55	6.351873e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028757	MtrunA17_Chr5g0436501	98.524	271	4	0	188	458	1	271	0.0	561
MsaG028757	MtrunA17_Chr5g0436481	97.326	187	5	0	1	187	1	187	2.28e-131	377
MsaG028757	MtrunA17_Chr7g0241761	62.150	214	72	2	190	396	11	222	5.28e-87	268
MsaG028757	MtrunA17_Chr5g0429521	61.386	202	71	3	189	385	4	203	7.18e-82	255
MsaG028757	MtrunA17_Chr3g0104071	64.322	199	58	4	203	390	27	223	1.02e-80	253
MsaG028757	MtrunA17_Chr1g0162081	51.832	191	76	4	212	389	10	197	3.03e-54	183
MsaG028757	MtrunA17_Chr1g0210471	49.733	187	80	4	212	391	22	201	7.75e-53	177
MsaG028757	MtrunA17_Chr4g0062741	42.381	210	81	5	214	388	8	212	1.36e-45	160
MsaG028757	MtrunA17_Chr8g0365011	43.229	192	79	4	212	389	6	181	2.23e-37	138
MsaG028757	MtrunA17_Chr2g0331031	61.111	90	33	1	307	396	104	191	5.95e-28	114
MsaG028757	MtrunA17_Chr6g0488471	69.863	73	20	1	306	378	91	161	1.98e-27	111
MsaG028757	MtrunA17_Chr4g0055931	64.198	81	27	1	307	387	90	168	1.88e-26	109
MsaG028757	MtrunA17_Chr8g0389241	60.714	84	30	1	303	386	96	176	2.36e-25	105
MsaG028757	MtrunA17_Chr5g0415191	60.000	90	34	1	299	388	89	176	1.24e-24	103
MsaG028757	MtrunA17_Chr4g0059701	71.186	59	17	0	300	358	72	130	2.27e-23	99.8
MsaG028757	MtrunA17_Chr5g0409841	49.524	105	49	1	279	379	55	159	7.39e-23	97.1
MsaG028757	MtrunA17_Chr8g0364741	40.984	122	66	2	212	329	8	127	1.18e-22	93.2
MsaG028757	MtrunA17_Chr5g0440741	53.933	89	39	2	292	379	81	168	6.37e-22	95.5
MsaG028757	MtrunA17_Chr5g0440731	58.904	73	29	1	308	379	101	173	9.72e-22	95.1
MsaG028757	MtrunA17_Chr3g0135671	51.429	105	44	4	277	378	58	158	1.01e-21	93.6
MsaG028757	MtrunA17_Chr1g0155181	55.882	68	30	0	209	276	2	69	3.81e-20	84.7
MsaG028757	MtrunA17_Chr3g0092621	57.971	69	27	1	312	380	84	150	1.25e-19	86.3
MsaG028757	MtrunA17_Chr3g0141601	53.846	65	30	0	212	276	5	69	1.08e-18	80.9
MsaG028757	MtrunA17_Chr6g0449111	52.941	68	32	0	209	276	5	72	4.30e-18	78.6
MsaG028757	MtrunA17_Chr5g0409831	58.904	73	29	1	308	379	73	145	4.32e-18	83.2
MsaG028757	MtrunA17_Chr5g0432921	33.000	200	119	7	213	405	20	211	6.80e-18	82.4
MsaG028757	MtrunA17_Chr7g0255841	53.731	67	31	0	218	284	16	82	2.20e-17	77.0
MsaG028757	MtrunA17_Chr3g0113121	40.764	157	79	7	235	383	21	171	2.47e-17	80.9
MsaG028757	MtrunA17_Chr8g0364731	60.294	68	27	0	322	389	11	78	2.72e-17	79.7
MsaG028757	MtrunA17_Chr6g0452791	57.627	59	25	0	312	370	80	138	6.05e-17	78.6
MsaG028757	MtrunA17_Chr5g0404101	45.333	75	41	0	202	276	7	81	7.32e-17	75.9
MsaG028757	MtrunA17_Chr3g0144631	52.381	63	30	0	214	276	16	78	7.98e-17	75.1
MsaG028757	MtrunA17_Chr5g0432941	30.288	208	113	7	213	405	21	211	2.76e-16	77.8
MsaG028757	MtrunA17_Chr3g0081631	47.945	73	37	1	205	276	1	73	3.32e-16	73.9
MsaG028757	MtrunA17_Chr8g0373131	49.231	65	32	1	212	276	3	66	7.58e-15	69.3
MsaG028757	MtrunA17_Chr8g0373111	49.231	65	32	1	212	276	3	66	8.22e-15	69.3
MsaG028757	MtrunA17_Chr8g0373121	49.231	65	32	1	212	276	3	66	8.22e-15	69.3
MsaG028757	MtrunA17_Chr8g0373101	50.820	61	29	1	214	274	5	64	1.75e-13	65.5
MsaG028757	MtrunA17_Chr1g0192061	55.714	70	29	2	314	382	63	131	6.53e-13	67.4
MsaG028757	MtrunA17_Chr5g0404301	50.820	61	29	1	214	274	4	63	6.60e-13	63.5
MsaG028757	MtrunA17_Chr3g0141641	41.270	63	37	0	214	276	3	65	2.33e-12	62.8
MsaG028757	MtrunA17_Chr8g0370121	50.000	50	25	0	309	358	97	146	2.15e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG028757	AT2G38090.1	59.716	211	69	2	190	386	5	213	3.20e-83	258
MsaG028757	AT3G11280.2	60.731	219	67	6	190	396	1	212	1.34e-77	243
MsaG028757	AT3G11280.1	60.731	219	67	6	190	396	1	212	1.34e-77	243
MsaG028757	AT5G05790.2	58.333	216	80	6	201	409	15	227	1.53e-76	241
MsaG028757	AT5G05790.1	58.333	216	80	6	201	409	15	227	1.53e-76	241
MsaG028757	AT5G58900.1	48.327	269	113	6	203	448	15	280	3.31e-74	235
MsaG028757	AT5G30495.1	54.011	187	84	2	1	187	1	185	1.33e-65	209
MsaG028757	AT5G30495.2	54.011	187	84	2	1	187	1	185	1.33e-65	209
MsaG028757	AT5G01200.1	52.995	217	79	6	188	383	1	215	1.49e-64	209
MsaG028757	AT1G54770.2	54.891	184	73	3	4	187	5	178	9.24e-62	199
MsaG028757	AT1G54770.1	54.891	184	73	3	4	187	5	178	9.24e-62	199
MsaG028757	AT5G08520.1	50.262	191	85	2	207	389	5	193	6.93e-52	177
MsaG028757	AT1G49010.1	45.050	202	81	4	208	381	2	201	1.95e-47	166
MsaG028757	AT5G04760.1	47.159	176	80	4	212	384	4	169	3.24e-44	154
MsaG028757	AT5G23650.1	45.745	188	79	4	207	379	6	185	2.88e-42	153
MsaG028757	AT3G10580.2	38.164	207	98	7	214	418	8	186	2.48e-33	126
MsaG028757	AT3G10580.1	38.164	207	98	7	214	418	8	186	6.70e-33	126
MsaG028757	AT4G09450.1	42.515	167	80	4	213	379	6	156	1.50e-30	117
MsaG028757	AT5G56840.1	61.905	84	31	1	307	390	86	168	1.73e-26	107
MsaG028757	AT5G47390.1	66.250	80	25	1	299	378	83	160	4.15e-26	109
MsaG028757	AT5G61620.1	54.639	97	42	1	283	379	79	173	1.55e-25	106
MsaG028757	AT1G19000.2	53.097	113	46	3	299	406	89	199	6.90e-24	101
MsaG028757	AT1G19000.1	53.097	113	46	3	299	406	89	199	6.90e-24	101
MsaG028757	AT1G70000.1	72.131	61	17	0	299	359	85	145	1.06e-23	100
MsaG028757	AT1G70000.2	72.131	61	17	0	299	359	85	145	1.06e-23	100
MsaG028757	AT3G16350.1	57.143	105	40	2	303	404	125	227	1.23e-23	102
MsaG028757	AT3G10590.1	42.222	180	79	11	214	379	7	175	1.61e-21	92.8
MsaG028757	AT1G74840.2	75.000	52	13	0	307	358	91	142	6.76e-21	92.0
MsaG028757	AT1G74840.1	75.000	52	13	0	307	358	91	142	1.00e-20	92.0
MsaG028757	AT1G75250.2	55.556	63	28	0	214	276	12	74	2.28e-17	77.8
MsaG028757	AT1G75250.1	55.556	63	28	0	214	276	12	74	3.79e-17	78.2
MsaG028757	AT4G39250.1	48.485	66	34	0	214	279	14	79	6.59e-17	76.6
MsaG028757	AT2G21650.1	47.761	67	35	0	214	280	14	80	1.59e-15	72.8
MsaG028757	AT2G18328.1	54.098	61	27	1	216	276	14	73	1.48e-13	66.2
MsaG028757	AT1G19510.1	51.724	58	28	0	219	276	17	74	1.57e-12	63.9
MsaG028757	AT3G10595.1	29.054	148	81	5	211	358	3	126	4.94e-12	65.1

Find 99 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCGATTCAGAATTGGTTAA+TGG	0.209686	5:+68116074	MsaT028757.1:CDS
GTCTCCTATGCAGCAGAAAA+TGG	0.222210	5:+68124386	MsaT028757.1:CDS
TATGCTAAGCTGAAAGTTTC+GGG	0.251091	5:+68124483	MsaT028757.1:CDS
TCGGGTTTCGCGACAGCTTC+TGG	0.263182	5:+68124501	MsaT028757.1:CDS
CTGATGGGACTTCTAAAGTA+TGG	0.266546	5:+68124156	MsaT028757.1:CDS
ACCAGGAATCGGAACATTTC+CGG	0.268577	5:-68123262	None:intergenic
GGCTCTTCCTAAGTACTTTC+AGG	0.275808	5:+68117947	MsaT028757.1:CDS
AGGAACCAAGTAGCACTATT+TGG	0.289225	5:+68116048	MsaT028757.1:CDS
CTCTTCTTGTAGTGCCGCTT+TGG	0.316933	5:-68117909	None:intergenic
GCCGGAAATGTTCCGATTCC+TGG	0.317971	5:+68123261	MsaT028757.1:CDS
TTATGCTAAGCTGAAAGTTT+CGG	0.326332	5:+68124482	MsaT028757.1:CDS
CTTCTCTACTCCATTCTGTT+TGG	0.332735	5:-68123089	None:intergenic
AGGAGACACATTGACTAATA+AGG	0.335368	5:-68124370	None:intergenic
ACATAAAATAAGGAGAATAA+AGG	0.337256	5:-68123035	None:intergenic
TGTGATTTAAGGTATGGATT+TGG	0.345223	5:+68123008	MsaT028757.1:intron
AATAGTGCTACTTGGTTCCT+TGG	0.355912	5:-68116045	None:intergenic
TGATGTGATTAAGCAGTATA+GGG	0.364968	5:+68123209	MsaT028757.1:CDS
TTCAGATCTTTCTGCAACTC+AGG	0.377777	5:-68116523	None:intergenic
AGAAGGCATGTGACTGTTAG+AGG	0.384719	5:+68123345	MsaT028757.1:CDS
CCCATTAACCAATTCTGAAT+CGG	0.389149	5:-68116075	None:intergenic
AAGATTGCTGAGATGATTCC+TGG	0.391655	5:+68123174	MsaT028757.1:CDS
CCGATTCAGAATTGGTTAAT+GGG	0.396125	5:+68116075	MsaT028757.1:CDS
TTGATGTGATTAAGCAGTAT+AGG	0.399334	5:+68123208	MsaT028757.1:CDS
TTGGAAACCCGATTCAGAAT+TGG	0.400416	5:+68116067	MsaT028757.1:CDS
TTTGTATCCAACATAAAATA+AGG	0.405866	5:-68123045	None:intergenic
ATAAGGCAAAAGGTTTCTTC+TGG	0.415949	5:+68124264	MsaT028757.1:CDS
CCATTAACCAATTCTGAATC+GGG	0.417188	5:-68116074	None:intergenic
GCAATCTTAAGCCATCTATC+TGG	0.417904	5:-68123159	None:intergenic
TAAAGTATGGCAAAGGCGAC+TGG	0.439380	5:+68124169	MsaT028757.1:CDS
CATAAAATAAGGAGAATAAA+GGG	0.445828	5:-68123034	None:intergenic
GATTAAGCAGTATAGGGAAT+TGG	0.449715	5:+68123215	MsaT028757.1:CDS
TGCAACTCAGGGGTGATGGT+TGG	0.453667	5:-68116511	None:intergenic
GCATGACTTGCTACTTGTGT+TGG	0.455619	5:-68124228	None:intergenic
TGCTCTTGCTATATATGATA+AGG	0.457525	5:+68123131	MsaT028757.1:CDS
TTTCTGCAACTCAGGGGTGA+TGG	0.466026	5:-68116515	None:intergenic
CCATCTCTGTCAGAGGCAAC+AGG	0.468656	5:+68115996	MsaT028757.1:CDS
ATAAGGATACACCAGATAGA+TGG	0.475384	5:+68123148	MsaT028757.1:CDS
ATGCACAAAAGTACTACATA+AGG	0.480834	5:+68124247	MsaT028757.1:CDS
GAAGAGCACAGGCGACCAGC+TGG	0.484252	5:+68118724	MsaT028757.1:CDS
AGAGGAAGAAAGGTGTTCCT+TGG	0.489408	5:+68123379	MsaT028757.1:CDS
CAGCAAGTTAAGGATACTGC+TGG	0.495586	5:+68116146	MsaT028757.1:CDS
GGTTTCCAAATAGTGCTACT+TGG	0.497133	5:-68116053	None:intergenic
GTTACTGAGACAGCAAGTTA+AGG	0.500220	5:+68116136	MsaT028757.1:CDS
GAACTTGCCTGAAAGTACTT+AGG	0.507435	5:-68117954	None:intergenic
TTCGAGAAATTGAAGAGCAC+AGG	0.509470	5:+68118713	MsaT028757.1:CDS
AAAGTACTACATAAGGCAAA+AGG	0.510073	5:+68124254	MsaT028757.1:CDS
AATAAGGACTTGTTATCTGA+TGG	0.512003	5:-68124354	None:intergenic
TGCCATGAGAGGCCAACCAC+TGG	0.514796	5:-68115957	None:intergenic
AGCCAGTGGTTGGCCTCTCA+TGG	0.517461	5:+68115955	MsaT028757.1:CDS
GAGGCAAGATAACCAGGAAT+CGG	0.520347	5:-68123273	None:intergenic
TGATGGATCACCACAAGGGC+AGG	0.524353	5:+68124431	MsaT028757.1:CDS
GCCTCTGACAGAGATGGAAT+AGG	0.528568	5:-68115990	None:intergenic
TCAGATCTTTCTGCAACTCA+GGG	0.532610	5:-68116522	None:intergenic
GAAGAGTGGTTCAAAATCAA+AGG	0.533957	5:+68117926	MsaT028757.1:CDS
TCTCCTATGCAGCAGAAAAT+GGG	0.535185	5:+68124387	MsaT028757.1:CDS
GAAAGATCTGAAGTTACTAC+AGG	0.536271	5:+68116534	MsaT028757.1:CDS
AGTACTAGCCACTACAATGA+TGG	0.540673	5:+68124414	MsaT028757.1:CDS
TCTCCACTGGACTACTACTC+AGG	0.541583	5:+68118075	MsaT028757.1:CDS
TCTGATCATGAGAGGAAGAA+AGG	0.544690	5:+68123369	MsaT028757.1:CDS
TCAGGGGTGATGGTTGGAGC+AGG	0.547716	5:-68116505	None:intergenic
AAAGAAGAGGCAAGATAACC+AGG	0.552310	5:-68123279	None:intergenic
AAGAGAGTTACCAAACAGAA+TGG	0.556624	5:+68123079	MsaT028757.1:CDS
GATCAGGTGGGAACAATGGT+AGG	0.566845	5:+68118051	MsaT028757.1:intron
CCTGTTGCCTCTGACAGAGA+TGG	0.568323	5:-68115996	None:intergenic
GCAACTGTGGTTGCCATGAG+AGG	0.580258	5:-68115968	None:intergenic
AGGCAAAAGGTTTCTTCTGG+AGG	0.582472	5:+68124267	MsaT028757.1:CDS
ACTATGATGGAAACAGAAGA+AGG	0.582942	5:+68123328	MsaT028757.1:CDS
AACAATGGTAGGTTCTCCAC+TGG	0.584771	5:+68118062	MsaT028757.1:CDS
TCATAGAGATCCTGCCCTTG+TGG	0.585420	5:-68124441	None:intergenic
CTTGGACTGAAGAGGAACAT+AGG	0.585629	5:+68123397	MsaT028757.1:CDS
CTATGTTCCTCTTCAGTCCA+AGG	0.586053	5:-68123396	None:intergenic
GTTTGAGATGTAGTATGAGA+TGG	0.586415	5:-68116023	None:intergenic
CTATGCAGCAGAAAATGGGT+TGG	0.587490	5:+68124391	MsaT028757.1:CDS
AGGATACTGCTGGGAAACAA+TGG	0.589108	5:+68116156	MsaT028757.1:CDS
AGGTGTTCCTTGGACTGAAG+AGG	0.592742	5:+68123389	MsaT028757.1:CDS
GACCAGCTGGGAATGAAAAG+TGG	0.593708	5:+68118737	MsaT028757.1:CDS
AAGAGCACAGGCGACCAGCT+GGG	0.595474	5:+68118725	MsaT028757.1:CDS
TCATACTACATCTCAAACCA+AGG	0.595482	5:+68116028	MsaT028757.1:CDS
AATGAAAAGTGGAAGATCCA+AGG	0.598156	5:+68118748	MsaT028757.1:CDS
GCTTCTGGAGATTTCAACAA+AGG	0.599003	5:+68124516	MsaT028757.1:CDS
GTTGTGATATCATGAATGCT+TGG	0.600782	5:-68124306	None:intergenic
GCCTATTCCATCTCTGTCAG+AGG	0.611837	5:+68115989	MsaT028757.1:CDS
GATGTGAGTGAAATTGAGGC+CGG	0.613302	5:+68123243	MsaT028757.1:CDS
TACAATGATGGATCACCACA+AGG	0.622159	5:+68124426	MsaT028757.1:CDS
ATATGATCAGGTGGGAACAA+TGG	0.625453	5:+68118047	MsaT028757.1:intron
AACTTCAAAGGTGAAAGAAG+AGG	0.626616	5:-68123292	None:intergenic
ACATCAATAACAGTCTTACC+AGG	0.630015	5:-68123192	None:intergenic
AGCAAGTTAAGGATACTGCT+GGG	0.632020	5:+68116147	MsaT028757.1:CDS
TTAGAGGTTCTGATCATGAG+AGG	0.633143	5:+68123361	MsaT028757.1:CDS
CTGCCTGAGTAGTAGTCCAG+TGG	0.633497	5:-68118078	None:intergenic
GTGGTGATCCATCATTGTAG+TGG	0.633599	5:-68124422	None:intergenic
GCAGATTAACAAATCGTGAG+AGG	0.634152	5:+68118097	MsaT028757.1:CDS
AGAAGATGTGAGTGAAATTG+AGG	0.635385	5:+68123239	MsaT028757.1:CDS
GGACTTCTAAAGTATGGCAA+AGG	0.636983	5:+68124162	MsaT028757.1:CDS
AAGCGGCACTACAAGAAGAG+TGG	0.668597	5:+68117912	MsaT028757.1:CDS
CAGATCTTTCTGCAACTCAG+GGG	0.673749	5:-68116521	None:intergenic
AGAGATGGAATAGGCAACTG+TGG	0.719411	5:-68115981	None:intergenic
ACAATGATGGATCACCACAA+GGG	0.720384	5:+68124427	MsaT028757.1:CDS
GGGATGCTATTGATCCAAAG+CGG	0.746074	5:+68117895	MsaT028757.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	68115942	68124566	68115942	ID=MsaG028757
Chr5	mRNA	68115942	68124566	68115942	ID=MsaT028757.1;Parent=MsaG028757
Chr5	exon	68115942	68116177	68115942	ID=MsaT028757.1.exon1;Parent=MsaT028757.1
Chr5	CDS	68115942	68116177	68115942	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68116495	68116555	68116495	ID=MsaT028757.1.exon2;Parent=MsaT028757.1
Chr5	CDS	68116495	68116555	68116495	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68117891	68117968	68117891	ID=MsaT028757.1.exon3;Parent=MsaT028757.1
Chr5	CDS	68117891	68117968	68117891	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68118057	68118172	68118057	ID=MsaT028757.1.exon4;Parent=MsaT028757.1
Chr5	CDS	68118057	68118172	68118057	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68118702	68118769	68118702	ID=MsaT028757.1.exon5;Parent=MsaT028757.1
Chr5	CDS	68118702	68118769	68118702	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68123019	68123418	68123019	ID=MsaT028757.1.exon6;Parent=MsaT028757.1
Chr5	CDS	68123019	68123418	68123019	ID=cds.MsaT028757.1;Parent=MsaT028757.1
Chr5	exon	68124149	68124566	68124149	ID=MsaT028757.1.exon7;Parent=MsaT028757.1
Chr5	CDS	68124149	68124566	68124149	ID=cds.MsaT028757.1;Parent=MsaT028757.1

Gene Sequence

>MsaT028757.1

ATGTTAAAGAAAAAGCCAGTGGTTGGCCTCTCATGGCAACCACAGTTGCCTATTCCATCTCTGTCAGAGGCAACAGGTGATCCATCTCATACTACATCTCAAACCAAGGAACCAAGTAGCACTATTTGGAAACCCGATTCAGAATTGGTTAATGGGCTGTTTGTTCCTCCAAATGATCCGAGAAAATTAAACAAGTTACTGAGACAGCAAGTTAAGGATACTGCTGGGAAACAATGGTTTGACATGCCTGCTCCAACCATCACCCCTGAGTTGCAGAAAGATCTGAAGTTACTACAGTTGAGGGATGCTATTGATCCAAAGCGGCACTACAAGAAGAGTGGTTCAAAATCAAAGGCTCTTCCTAAGTACTTTCAGGTGGGAACAATGGTAGGTTCTCCACTGGACTACTACTCAGGCAGATTAACAAATCGTGAGAGGAAAGAATCAATTGCAGATGAGTTGCTTTCCGATCAAAACTTTTCAGCTTACAGGAAGCGGAAGGTTCGAGAAATTGAAGAGCACAGGCGACCAGCTGGGAATGAAAAGTGGAAGATCCAAGGTATGGATTTGGAAACCCTTTATTCTCCTTATTTTATGTTGGATACAAATTGGCTTGTTGAAGAGAGTTACCAAACAGAATGGAGTAGAGAAGATAACAAGAAGTTTGAAAGTGCTCTTGCTATATATGATAAGGATACACCAGATAGATGGCTTAAGATTGCTGAGATGATTCCTGGTAAGACTGTTATTGATGTGATTAAGCAGTATAGGGAATTGGTAGAAGATGTGAGTGAAATTGAGGCCGGAAATGTTCCGATTCCTGGTTATCTTGCCTCTTCTTTCACCTTTGAAGTTGTCGAAAAACAAAACTATGATGGAAACAGAAGAAGGCATGTGACTGTTAGAGGTTCTGATCATGAGAGGAAGAAAGGTGTTCCTTGGACTGAAGAGGAACATAGACGTTTTCTGATGGGACTTCTAAAGTATGGCAAAGGCGACTGGAGAAACATCTCGCGCAATTTCGTCGTGACAAAGACTCCAACACAAGTAGCAAGTCATGCACAAAAGTACTACATAAGGCAAAAGGTTTCTTCTGGAGGCAAAGATAAAAGAAGACCAAGCATTCATGATATCACAACTGTTACTCTCACAGAAACTTCTTCTCCATCAGATAACAAGTCCTTATTAGTCAATGTGTCTCCTATGCAGCAGAAAATGGGTTGGAGTACTAGCCACTACAATGATGGATCACCACAAGGGCAGGATCTCTATGACTGCTCTTTTCATGAGGCTTATGCTAAGCTGAAAGTTTCGGGTTTCGCGACAGCTTCTGGAGATTTCAACAAAGGAGCTGTTTTTGGTATTCATGCACTGTAA

Protein sequence

>MsaT028757.1

MLKKKPVVGLSWQPQLPIPSLSEATGDPSHTTSQTKEPSSTIWKPDSELVNGLFVPPNDPRKLNKLLRQQVKDTAGKQWFDMPAPTITPELQKDLKLLQLRDAIDPKRHYKKSGSKSKALPKYFQVGTMVGSPLDYYSGRLTNRERKESIADELLSDQNFSAYRKRKVREIEEHRRPAGNEKWKIQGMDLETLYSPYFMLDTNWLVEESYQTEWSREDNKKFESALAIYDKDTPDRWLKIAEMIPGKTVIDVIKQYRELVEDVSEIEAGNVPIPGYLASSFTFEVVEKQNYDGNRRRHVTVRGSDHERKKGVPWTEEEHRRFLMGLLKYGKGDWRNISRNFVVTKTPTQVASHAQKYYIRQKVSSGGKDKRRPSIHDITTVTLTETSSPSDNKSLLVNVSPMQQKMGWSTSHYNDGSPQGQDLYDCSFHEAYAKLKVSGFATASGDFNKGAVFGIHAL*