AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028925


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028925 MtrunA17_Chr5g0438661 94.884 430 22 0 1 430 33 462 0.0 822
MsaG028925 MtrunA17_Chr7g0258521 36.655 281 149 8 34 306 15 274 2.10e-41 152
MsaG028925 MtrunA17_Chr8g0335121 27.299 348 237 7 48 388 18 356 4.54e-30 122
MsaG028925 MtrunA17_Chr7g0249711 24.148 352 257 5 46 390 30 378 4.39e-23 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028925 AT4G19650.2 44.413 349 193 1 68 416 79 426 8.87e-107 328
MsaG028925 AT4G19650.1 41.311 351 203 2 68 416 60 409 2.48e-93 293
MsaG028925 AT5G06810.1 33.506 385 251 3 22 404 24 405 1.40e-65 220
MsaG028925 AT5G45113.1 39.919 248 149 0 168 415 1 248 9.02e-60 201
MsaG028925 AT5G06811.1 32.905 389 242 5 22 404 24 399 8.71e-59 202
MsaG028925 AT3G60400.1 26.648 349 241 6 42 386 9 346 3.56e-32 129
MsaG028925 AT5G45115.2 47.692 65 34 0 57 121 58 122 2.15e-11 61.6
MsaG028925 AT5G45115.1 47.692 65 34 0 57 121 58 122 2.15e-11 61.6

Find 67 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAGAAAGAGCGGGAATT+TGG 0.207920 5:+70680100 None:intergenic
TTGAGCAAACATTCGATAAA+AGG 0.208563 5:-70679160 MsaT028925.1:CDS
AAAAGGTTCAAATTCGTTAA+TGG 0.216359 5:+70679873 None:intergenic
ATTTCAAGATTATGAAGATT+TGG 0.259240 5:-70679653 MsaT028925.1:CDS
GAATTGGGATTGAGAGTAAA+TGG 0.269440 5:-70679528 MsaT028925.1:CDS
ATTCTGCATAAATCAGCTTT+TGG 0.277612 5:+70679106 None:intergenic
CTTGATTGGTTGAAAAGAAT+TGG 0.282428 5:-70679544 MsaT028925.1:CDS
TTTCAAGATTATGAAGATTT+GGG 0.302755 5:-70679652 MsaT028925.1:CDS
TCCATTGGAGCTTATTAGTT+TGG 0.304432 5:-70679071 MsaT028925.1:CDS
AAAGGTTCAAATTCGTTAAT+GGG 0.316048 5:+70679874 None:intergenic
GTGGCGCGTTTGATGTAAAT+TGG 0.317940 5:+70680078 None:intergenic
AGGAATTAATCACACGGAAT+TGG 0.320142 5:-70679839 MsaT028925.1:CDS
AGGAAGCTGTTCTTGTTTCT+AGG 0.327574 5:-70679358 MsaT028925.1:CDS
ATCGTCAGGGATGTTGATTT+TGG 0.327979 5:+70679939 None:intergenic
TGTTTGTTGTCCTTTGCTTT+TGG 0.333098 5:-70679599 MsaT028925.1:CDS
TGGAGAAAGAGCGGGAATTT+GGG 0.341540 5:+70680101 None:intergenic
AGAGCGGTTTGTAAGGAGTT+AGG 0.349298 5:-70679133 MsaT028925.1:CDS
AGTTTGTTGTTGTTCTTGAT+TGG 0.351137 5:-70679558 MsaT028925.1:CDS
TTTAATAAGCGGGTCACAAT+AGG 0.351904 5:+70679012 None:intergenic
TTTCATGTATTGTGTTATCA+TGG 0.353729 5:-70679757 MsaT028925.1:CDS
TTGATTGGTTGAAAAGAATT+GGG 0.361761 5:-70679543 MsaT028925.1:CDS
CAAAGAAAATAACAACCTTT+AGG 0.370775 5:+70679805 None:intergenic
AAATGGAAGAAGATGGGTTT+TGG 0.374297 5:+70680160 None:intergenic
GGTGCAAGAAATTGGGGTTT+TGG 0.381606 5:+70680181 None:intergenic
TTGAAAGAAATGGAAGAAGA+TGG 0.405404 5:+70680153 None:intergenic
CGCTTATTAAATTGAGAAAA+CGG 0.406330 5:-70679000 MsaT028925.1:CDS
TGGGTATGGAAATGGAGTTT+TGG 0.409236 5:-70679683 MsaT028925.1:CDS
TCTAGGTCGGTTTATTAAGT+CGG 0.428427 5:-70679341 MsaT028925.1:CDS
AGGTTATAGAGCGGTTTGTA+AGG 0.431374 5:-70679140 MsaT028925.1:CDS
CTAGGTCGGTTTATTAAGTC+GGG 0.441636 5:-70679340 MsaT028925.1:CDS
TTCATGTATTGTGTTATCAT+GGG 0.455291 5:-70679756 MsaT028925.1:CDS
AGACCTTCCAACAACAAATT+CGG 0.457193 5:+70679385 None:intergenic
TGAAAGAAATGGAAGAAGAT+GGG 0.458028 5:+70680154 None:intergenic
ACACTTACTCGACAATATAT+CGG 0.459640 5:+70679276 None:intergenic
CATATGTATGATTTGTTCAA+GGG 0.474363 5:-70679412 MsaT028925.1:CDS
ATCTTCGCCATTCGATTCCT+CGG 0.482527 5:+70679730 None:intergenic
ACATATGTATGATTTGTTCA+AGG 0.498470 5:-70679413 MsaT028925.1:CDS
GATCCGAATTTGTTGTTGGA+AGG 0.498949 5:-70679388 MsaT028925.1:CDS
TTAGGGTTGAAGAGAAAGAA+TGG 0.499067 5:+70680234 None:intergenic
AGCTGTTCTTGTTTCTAGGT+CGG 0.500050 5:-70679354 MsaT028925.1:CDS
CTTCTAAACAGAAACATAAG+AGG 0.503889 5:-70679048 MsaT028925.1:CDS
CTAGTTCTAGCACTTATAGC+TGG 0.504313 5:-70679489 MsaT028925.1:CDS
ATTTGTTGTTGGAAGGTCTA+GGG 0.509859 5:-70679381 MsaT028925.1:CDS
AATTTGTTGTTGGAAGGTCT+AGG 0.516290 5:-70679382 MsaT028925.1:CDS
GTAAATTGGTGGAGAAAGAG+CGG 0.528173 5:+70680092 None:intergenic
TCATGTATTGTGTTATCATG+GGG 0.535058 5:-70679755 MsaT028925.1:CDS
TAGGGTTGAAGAGAAAGAAT+GGG 0.541091 5:+70680235 None:intergenic
ATGAGGAAATTGATGAAATG+TGG 0.545861 5:+70679964 None:intergenic
GCCAAACTAATAAGCTCCAA+TGG 0.546201 5:+70679070 None:intergenic
AATCATAAACGATGATCCAT+TGG 0.546725 5:-70679086 MsaT028925.1:CDS
AGAAAGAGCGGGAATTTGGG+TGG 0.556121 5:+70680104 None:intergenic
TAAATTGGTGGAGAAAGAGC+GGG 0.577774 5:+70680093 None:intergenic
CAGAAACATAAGAGGAGTGG+AGG 0.579021 5:-70679040 MsaT028925.1:CDS
TGTGTTATCATGGGGTTCCG+AGG 0.589302 5:-70679747 MsaT028925.1:CDS
GAAAGTGAAAGAGTTCCCAT+TGG 0.599604 5:+70680273 None:intergenic
AAACAGAAACATAAGAGGAG+TGG 0.607869 5:-70679043 MsaT028925.1:CDS
TGTTGTTGGAAGGTCTAGGG+AGG 0.611420 5:-70679378 MsaT028925.1:CDS
GCGCGTTTGATGTAAATTGG+TGG 0.612365 5:+70680081 None:intergenic
TTCGATAAAAGGTTATAGAG+CGG 0.623688 5:-70679149 MsaT028925.1:CDS
TGGGGTTCCGAGGAATCGAA+TGG 0.633090 5:-70679737 MsaT028925.1:CDS
AGAAACATAAGAGGAGTGGA+GGG 0.636056 5:-70679039 MsaT028925.1:CDS
ATATGTATGATTTGTTCAAG+GGG 0.636636 5:-70679411 MsaT028925.1:CDS
ACATCACCAACCAAAAGCAA+AGG 0.637648 5:+70679589 None:intergenic
AAAATCAACATCCCTGACGA+TGG 0.646277 5:-70679937 MsaT028925.1:CDS
TATATTGTCGAGTAAGTGTG+TGG 0.647992 5:-70679272 MsaT028925.1:CDS
TATGTATGATTTGTTCAAGG+GGG 0.703088 5:-70679410 MsaT028925.1:CDS
GAGTTTAGGAATTAATCACA+CGG 0.713083 5:-70679845 MsaT028925.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 70678988 70680292 70678988 ID=MsaG028925
Chr5 mRNA 70678988 70680292 70678988 ID=MsaT028925.1;Parent=MsaG028925
Chr5 exon 70678988 70680292 70678988 ID=MsaT028925.1.exon1;Parent=MsaT028925.1
Chr5 CDS 70678988 70680292 70678988 ID=cds.MsaT028925.1;Parent=MsaT028925.1
Gene Sequence

>MsaT028925.1

ATGGGAACTCTTTCACTTTCTCTGCATAATCTCAACCCATTCTTTCTCTTCAACCCTAATTCCACACCCCAAAACCCCAATTTTTCTCACCAAAACCCCAATTTCTTGCACCAAAACCCATCTTCTTCCATTTCTTTCAAAAATTCAACCTTGAACTGTTCAAAATCCACCCAAATTCCCGCTCTTTCTCCACCAATTTACATCAAACGCGCCACGAAAATTGAAGCACAACGTGCTCTATTTGAATACCTTCACTACACACAAAACTACACTTTCTCAGATGCTGAATTCATCTCCAAAAACTCTCCACATTTCATCAATTTCCTCATCTCCAAAATCAACATCCCTGACGATGGCGACGTTTTTCGTGCTCTCAGCAGGTATCTTATGTATCATCCCATTAACGAATTTGAACCTTTTTTAGAGAGTTTAGGAATTAATCACACGGAATTGGAAAAATTTCTACCTAAAGGTTGTTATTTTCTTTGTGATGATAGTGTTTTAGTTGATAATTTTCATGTATTGTGTTATCATGGGGTTCCGAGGAATCGAATGGCGAAGATTTATACCGAAGCAAGAGAAATTTTTGGGTATGGAAATGGAGTTTTGGAAAAGAAATTTCAAGATTATGAAGATTTGGGTTTGTGTAAATCATCTCTTATCAAACTTTTTGTTTGTTGTCCTTTGCTTTTGGTTGGTGATGTTGATTCTGAGTTTGTTGTTGTTCTTGATTGGTTGAAAAGAATTGGGATTGAGAGTAAATGGTTTGTGAGTTGTATGTCTAGTTCTAGCACTTATAGCTGGAAAAGAATGATTGAGACTTTAGAGTTTTTTCATCAAGTGGGTTATTCTGAGAAACATATGTATGATTTGTTCAAGGGGGATCCGAATTTGTTGTTGGAAGGTCTAGGGAGGAAGCTGTTCTTGTTTCTAGGTCGGTTTATTAAGTCGGGTGTTGACGTGAATGTTGTCTGTTCTTGTTTCATAGAGCATTCCGATATATTGTCGAGTAAGTGTGTGGAAAATTTGATGAGTGTTATTTCTTTTTTGTATAATATTCGAATGGAACAAGATGATATTGCACATGTTTTGTCTAACTACATGCATATCTTGAGCAAACATTCGATAAAAGGTTATAGAGCGGTTTGTAAGGAGTTAGGAGTTCCAAAAGCTGATTTATGCAGAATCATAAACGATGATCCATTGGAGCTTATTAGTTTGGCTTCTAAACAGAAACATAAGAGGAGTGGAGGGCAAACCTATTGTGACCCGCTTATTAAATTGAGAAAACGGCTTTCTTGCTGA

Protein sequence

>MsaT028925.1

MGTLSLSLHNLNPFFLFNPNSTPQNPNFSHQNPNFLHQNPSSSISFKNSTLNCSKSTQIPALSPPIYIKRATKIEAQRALFEYLHYTQNYTFSDAEFISKNSPHFINFLISKINIPDDGDVFRALSRYLMYHPINEFEPFLESLGINHTELEKFLPKGCYFLCDDSVLVDNFHVLCYHGVPRNRMAKIYTEAREIFGYGNGVLEKKFQDYEDLGLCKSSLIKLFVCCPLLLVGDVDSEFVVVLDWLKRIGIESKWFVSCMSSSSTYSWKRMIETLEFFHQVGYSEKHMYDLFKGDPNLLLEGLGRKLFLFLGRFIKSGVDVNVVCSCFIEHSDILSSKCVENLMSVISFLYNIRMEQDDIAHVLSNYMHILSKHSIKGYRAVCKELGVPKADLCRIINDDPLELISLASKQKHKRSGGQTYCDPLIKLRKRLSC*