AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG028955

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028955 MtrunA17_Chr5g0438991 90.437 962 75 6 50 998 1 958 0.0 1785
MsaG028955 MtrunA17_Chr3g0138411 65.579 1011 213 11 104 1014 231 1206 0.0 1301
MsaG028955 MtrunA17_Chr3g0138411 50.000 114 27 3 1 84 1 114 2.79e-16 84.3
MsaG028955 MtrunA17_Chr3g0133461 68.665 951 194 12 160 1014 401 1343 0.0 1278
MsaG028955 MtrunA17_Chr3g0133461 72.549 51 14 0 37 87 14 64 1.98e-12 72.0
MsaG028955 MtrunA17_Chr6g0456861 81.209 761 123 10 251 1007 28 772 0.0 1253
MsaG028955 MtrunA17_Chr4g0000031 46.552 812 365 12 257 1006 891 1695 0.0 725
MsaG028955 MtrunA17_Chr6g0456851 50.806 868 259 24 15 762 11 830 0.0 721
MsaG028955 MtrunA17_Chr6g0456851 65.672 67 23 0 807 873 807 873 1.39e-19 94.7
MsaG028955 MtrunA17_Chr6g0456931 71.951 246 68 1 478 723 1 245 3.66e-123 375
MsaG028955 MtrunA17_Chr5g0401991 78.696 230 36 4 708 933 1 221 5.38e-116 355
MsaG028955 MtrunA17_Chr5g0402001 88.119 101 12 0 556 656 1 101 2.41e-57 192
MsaG028955 MtrunA17_Chr5g0439001 83.158 95 15 1 658 752 1 94 5.20e-45 158
MsaG028955 MtrunA17_Chr1g0169961 68.103 116 36 1 251 366 54 168 1.35e-44 158
MsaG028955 MtrunA17_Chr6g0456791 50.867 173 57 2 140 312 1 145 1.31e-35 133
MsaG028955 MtrunA17_Chr6g0456921 49.682 157 19 5 556 711 1 98 4.70e-34 134
MsaG028955 MtrunA17_Chr6g0456911 89.091 55 6 0 819 873 6 60 1.51e-26 103
MsaG028955 MtrunA17_Chr6g0456941 39.224 232 68 9 93 305 54 231 2.04e-26 108
MsaG028955 MtrunA17_Chr0c02g0489811 87.719 57 7 0 817 873 5 61 3.91e-26 102
MsaG028955 MtrunA17_Chr7g0229221 84.211 57 9 0 817 873 5 61 8.12e-25 99.0
MsaG028955 MtrunA17_Chr0c02g0489731 80.702 57 11 0 817 873 5 61 2.28e-23 94.7
MsaG028955 MtrunA17_Chr0c02g0489721 85.106 47 7 0 747 793 1 47 1.38e-22 91.7
MsaG028955 MtrunA17_Chr7g0229231 85.106 47 7 0 747 793 1 47 1.38e-22 91.7
MsaG028955 MtrunA17_Chr0c02g0489821 86.667 45 6 0 749 793 1 45 1.19e-21 89.0
MsaG028955 MtrunA17_Chr6g0456781 60.563 71 19 3 338 407 25 87 8.86e-17 78.2
MsaG028955 MtrunA17_Chr6g0456771 52.381 84 26 1 217 300 27 96 2.41e-16 75.5
MsaG028955 MtrunA17_Chr5g0402011 90.323 31 3 0 402 432 21 51 9.43e-14 67.8
MsaG028955 MtrunA17_Chr5g0401281 38.889 72 43 1 927 997 279 350 4.48e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG028955 AT1G79000.3 45.402 859 389 16 218 1006 811 1659 0.0 748
MsaG028955 AT1G79000.1 45.402 859 389 16 218 1006 835 1683 0.0 748
MsaG028955 AT1G79000.2 45.402 859 389 16 218 1006 835 1683 0.0 748
MsaG028955 AT1G16710.2 45.773 828 375 13 247 1006 842 1663 0.0 727
MsaG028955 AT1G16710.3 45.773 828 375 13 247 1006 845 1666 0.0 726
MsaG028955 AT1G16710.10 45.773 828 375 13 247 1006 845 1666 0.0 726
MsaG028955 AT1G16710.8 45.773 828 375 13 247 1006 845 1666 0.0 726
MsaG028955 AT1G16710.1 45.773 828 375 13 247 1006 871 1692 0.0 726
MsaG028955 AT1G16710.7 45.773 828 375 13 247 1006 845 1666 0.0 726
MsaG028955 AT1G16710.6 45.773 828 375 13 247 1006 806 1627 0.0 726
MsaG028955 AT1G16710.4 45.773 828 375 13 247 1006 806 1627 0.0 726
MsaG028955 AT1G16710.5 45.773 828 375 13 247 1006 806 1627 0.0 726
MsaG028955 AT3G12980.1 42.759 870 403 17 222 1006 796 1655 0.0 694
MsaG028955 AT3G12980.2 42.759 870 403 17 222 1006 715 1574 0.0 692
MsaG028955 AT1G55970.1 42.454 815 390 15 262 1006 652 1457 0.0 628
MsaG028955 AT1G55970.2 42.454 815 376 16 262 1006 652 1443 0.0 619
MsaG028955 AT1G67220.1 42.561 773 366 14 280 986 600 1360 0.0 613
MsaG028955 AT1G16710.9 49.827 578 267 9 247 804 845 1419 1.99e-180 564
MsaG028955 AT5G67480.2 42.308 78 42 2 929 1005 286 361 1.75e-11 67.8
MsaG028955 AT5G67480.1 42.308 78 42 2 929 1005 275 350 1.85e-11 67.8
MsaG028955 AT5G67480.3 42.308 78 42 2 929 1005 275 350 1.85e-11 67.8

Find 179 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTCTTTGTCCTTCTTTC+AGG 0.063800 5:+71036048 None:intergenic
TTCCGAGTCTTTGCAATTTC+TGG 0.071145 5:+71034240 None:intergenic
TTTCTTCCTGCTACTGAAAA+AGG 0.118664 5:-71035531 MsaT028955.1:CDS
AACAGAGAACTTATTATTAT+TGG 0.166126 5:-71037881 MsaT028955.1:CDS
GTTTCACAACTTGCTATATA+TGG 0.181117 5:-71035856 MsaT028955.1:CDS
GGAATGTATGTCCAGGAATT+TGG 0.199160 5:-71036141 MsaT028955.1:CDS
CAAGCCTTCCAAAGACATTT+TGG 0.200018 5:-71035346 MsaT028955.1:CDS
GAAAGTAAATAAGCAGTTTC+TGG 0.202627 5:-71036391 MsaT028955.1:CDS
CACTGAATGCAAGAAGTTTC+AGG 0.206037 5:-71035071 MsaT028955.1:CDS
AGAGTAGATTGTTTGAAAAT+AGG 0.206122 5:-71034730 MsaT028955.1:CDS
CACTCGAATTTCACACTTAC+TGG 0.209713 5:+71034315 None:intergenic
TGTCAATTCCCACTGATATT+TGG 0.217704 5:+71037969 None:intergenic
GCCTGTGCTCCTTCAGAAAA+AGG 0.229084 5:-71035833 MsaT028955.1:CDS
CGGCGTAGCTTATCATTCTT+TGG 0.248851 5:+71035767 None:intergenic
GAGTAGATTGTTTGAAAATA+GGG 0.252944 5:-71034729 MsaT028955.1:CDS
CCAGACATCAGGAAAATTAT+TGG 0.253501 5:+71038150 None:intergenic
AGCATCATTTATTCTATTAG+AGG 0.271507 5:+71037649 None:intergenic
AACTCCCTCAAAAGGCTTAC+AGG 0.274257 5:-71037996 MsaT028955.1:CDS
CTTGTTAGGGGTTGTCATTT+AGG 0.277330 5:-71038759 MsaT028955.1:CDS
AAATCGAGAGGATGCTTGTT+AGG 0.290867 5:-71038773 None:intergenic
TTGCATCACCAAAATGTCTT+TGG 0.306673 5:+71035338 None:intergenic
CGCCTAAAAGAAATTGAAAA+TGG 0.318929 5:-71036828 MsaT028955.1:CDS
CATACTTTGATGGAGACTTC+TGG 0.341046 5:-71035479 MsaT028955.1:CDS
TGTTTGTCCTCTACATCAAT+AGG 0.343878 5:+71037758 None:intergenic
AATGTATACACAACGTTGAT+TGG 0.347633 5:+71036101 None:intergenic
TCTCGTTTGCCATACTTTGA+TGG 0.348808 5:-71035489 MsaT028955.1:CDS
CGCATTCAGAGCCAAATTCC+TGG 0.364387 5:+71036130 None:intergenic
AAGTGTGTTCATCAGAACTA+TGG 0.369353 5:+71034931 None:intergenic
AGTGTCAAATAGAACATTAA+AGG 0.370702 5:-71035382 MsaT028955.1:CDS
TCAACACCAGTATGTCAATC+TGG 0.372617 5:-71034560 MsaT028955.1:CDS
CCACAAAAGACGGAAAATAA+TGG 0.376394 5:-71038080 MsaT028955.1:CDS
CTTCTGTTTCAGAAAATTGA+AGG 0.379117 5:-71036183 MsaT028955.1:CDS
CAAGAACATTTCCATGGAAA+TGG 0.381561 5:-71037810 MsaT028955.1:CDS
GCCAGCATTGTAAGAAGATA+TGG 0.385181 5:-71034282 MsaT028955.1:CDS
CAAAATGTCTTTGGAAGGCT+TGG 0.387181 5:+71035347 None:intergenic
TGAGCGTGTGCAAGCTCTAT+TGG 0.399303 5:-71038263 MsaT028955.1:CDS
GAGGGAGTTATCTCGTGAAT+TGG 0.400635 5:+71038010 None:intergenic
GAGGACAAACAAGAAGGAAT+TGG 0.402147 5:-71037746 MsaT028955.1:CDS
CAGAAGTCTCCATCAAAGTA+TGG 0.405354 5:+71035480 None:intergenic
CTTATTATTATTGGTGATGA+TGG 0.411137 5:-71037872 MsaT028955.1:CDS
TTGTCATAGAGCTAAAGTTT+TGG 0.413031 5:-71034017 MsaT028955.1:CDS
TCTGAGAAAGGAATCTGTTC+AGG 0.416816 5:-71037573 MsaT028955.1:intron
TTTCACAACTTGCTATATAT+GGG 0.418086 5:-71035855 MsaT028955.1:CDS
TCAGTAGGGTAATTCTCCTC+TGG 0.420217 5:+71036360 None:intergenic
AATGTAGAATACCAAGTTGC+AGG 0.422686 5:-71034219 MsaT028955.1:intron
CACTTGCCAATTATGTGAAA+AGG 0.424866 5:-71037259 MsaT028955.1:CDS
TTCTTGATCTGAGAACAGTT+TGG 0.428112 5:+71034352 None:intergenic
TCCCAGACACTATCACTTCA+TGG 0.428163 5:+71035107 None:intergenic
TGATTCCCAGATTGACATAC+TGG 0.428638 5:+71034554 None:intergenic
AGTATGTCAATCTGGGAATC+AGG 0.429264 5:-71034552 MsaT028955.1:CDS
TCATGCAAGAGAGAGAAAAC+TGG 0.430015 5:-71036707 MsaT028955.1:CDS
TCAGGAGTCAAATGAGGAAA+TGG 0.431304 5:-71034534 MsaT028955.1:intron
ATGTATACACAACGTTGATT+GGG 0.434405 5:+71036102 None:intergenic
AAGTGTGAAATTCGAGTGAA+TGG 0.435360 5:-71034310 MsaT028955.1:CDS
TGATGAAGTACTTGAAGAAC+CGG 0.437567 5:-71037040 MsaT028955.1:intron
GGTGACAAAATCGCATTCAA+TGG 0.438280 5:-71037102 MsaT028955.1:CDS
ATCTCAATGCCGCGCAACTA+AGG 0.439497 5:-71034392 MsaT028955.1:CDS
TTTGAGCATCTGTCCCGTCC+TGG 0.446030 5:+71037717 None:intergenic
AATCGAGAGGATGCTTGTTA+GGG 0.446744 5:-71038772 None:intergenic
TTCAGGCCTAAAATACTTGA+CGG 0.457367 5:+71036065 None:intergenic
TTAGGGGTTGTCATTTAGGT+GGG 0.458581 5:-71038755 MsaT028955.1:CDS
TTACCGAAGGCAGCTAACTT+TGG 0.462740 5:-71036792 MsaT028955.1:CDS
CAAACTGAAACTGAGACTTC+TGG 0.468204 5:+71034687 None:intergenic
GCACCAAAGTTAGCTGCCTT+CGG 0.468246 5:+71036789 None:intergenic
GTTAGGGGTTGTCATTTAGG+TGG 0.468612 5:-71038756 MsaT028955.1:CDS
CGTCGTTTACCATGAGATAC+TGG 0.470570 5:-71036010 MsaT028955.1:intron
CGAGGTGCATACACTTTGTC+AGG 0.471850 5:-71034898 MsaT028955.1:intron
ATCAATAGGAACATGCAAAT+CGG 0.472111 5:+71037772 None:intergenic
TGCCATGAAGTGATAGTGTC+TGG 0.473473 5:-71035109 MsaT028955.1:CDS
CACGCTCATCCAACGTCTCC+AGG 0.474123 5:+71038278 None:intergenic
TGAGATATTTGCAACTATGC+TGG 0.474134 5:-71038482 MsaT028955.1:CDS
AAGATTCATTGGCCATTGAC+TGG 0.476711 5:+71033983 None:intergenic
AGACGGAAAATAATGGAGTC+TGG 0.478521 5:-71038073 MsaT028955.1:CDS
GGACGGGACAGATGCTCAAA+AGG 0.478902 5:-71037714 MsaT028955.1:CDS
GATCATCATGGAGGAATACT+TGG 0.481567 5:+71034649 None:intergenic
TTCTTCAATGTCCATTTCCA+TGG 0.483773 5:+71037799 None:intergenic
GAGCGTGTGCAAGCTCTATT+GGG 0.486853 5:-71038262 MsaT028955.1:CDS
GATCTATAAGAGAATTCAGT+AGG 0.487595 5:+71036345 None:intergenic
TCACAGTAGGGTTGTGCAGA+TGG 0.500457 5:+71034621 None:intergenic
TACAAAAGAAAACTTCTTCG+TGG 0.500683 5:-71035158 MsaT028955.1:CDS
GCTGCCTTCGGTAAGGGAAA+AGG 0.502615 5:+71036801 None:intergenic
ACATACCTTTGAAGCAGCAT+TGG 0.504078 5:+71038444 None:intergenic
AAAGTTAGCTGCCTTCGGTA+AGG 0.504444 5:+71036794 None:intergenic
GGCAGCTAACTTTGGTGCTA+AGG 0.504810 5:-71036784 MsaT028955.1:CDS
CTCCAGAAATTGCAAAGACT+CGG 0.505505 5:-71034242 MsaT028955.1:CDS
ACCGAAGAAAATATTGTCGT+TGG 0.506532 5:-71035579 MsaT028955.1:CDS
GTGACAAAATCGCATTCAAT+GGG 0.506600 5:-71037101 MsaT028955.1:CDS
ACTTACCAATGCTGCTTCAA+AGG 0.507990 5:-71038449 MsaT028955.1:intron
AGTGTGAAATTCGAGTGAAT+GGG 0.509029 5:-71034309 MsaT028955.1:CDS
ACTTGCCAATTATGTGAAAA+GGG 0.510157 5:-71037258 MsaT028955.1:CDS
ACCATATCTTCTTACAATGC+TGG 0.510556 5:+71034281 None:intergenic
CACGAGATAACTCCCTCAAA+AGG 0.512602 5:-71038004 MsaT028955.1:CDS
GCATGTTCCTATTGATGTAG+AGG 0.514894 5:-71037765 MsaT028955.1:CDS
AACTGTGCTAAGTGACCACT+TGG 0.516161 5:-71036751 MsaT028955.1:CDS
AAGTTAGCTGCCTTCGGTAA+GGG 0.516343 5:+71036795 None:intergenic
CAGTAGGGTAATTCTCCTCT+GGG 0.517758 5:+71036361 None:intergenic
CTACAACAAGAAAGCGGATG+TGG 0.521340 5:-71036883 MsaT028955.1:CDS
CATTGATTGATTCGTTCACA+TGG 0.521465 5:-71037622 MsaT028955.1:CDS
TTTAACACAGATTACTTCTG+AGG 0.521977 5:-71037304 MsaT028955.1:intron
CGCCAGTCCATGGAGAATCC+TGG 0.529802 5:+71038672 None:intergenic
CCCTCAAAAGGCTTACAGGC+TGG 0.532637 5:-71037992 MsaT028955.1:CDS
TCTATACAAGACCAGACATC+AGG 0.532841 5:+71038139 None:intergenic
TTCAGTGTTGCCATCATGAG+TGG 0.535190 5:+71038180 None:intergenic
GAAGGCGACGGACCAGTCAA+TGG 0.537964 5:-71033995 MsaT028955.1:CDS
CAACACCAGTATGTCAATCT+GGG 0.540531 5:-71034559 MsaT028955.1:CDS
TACATTCTCACAATCTGCCT+CGG 0.541414 5:-71038221 MsaT028955.1:CDS
ACATGTTAGTAGTCTGAGAA+AGG 0.542692 5:-71037585 MsaT028955.1:CDS
ATTGCAACACACCTGCAACT+TGG 0.542761 5:+71034208 None:intergenic
ATCACCAAAATGTCTTTGGA+AGG 0.546733 5:+71035342 None:intergenic
TAGGGTTGTGCAGATGGTAA+AGG 0.549226 5:+71034627 None:intergenic
TGTATGTCCTCTTCGAGAGT+AGG 0.553262 5:+71037941 None:intergenic
ATCATTACGTTTAGTGTCGG+AGG 0.554837 5:+71034763 None:intergenic
AACCAGGATTCTCCATGGAC+TGG 0.558239 5:-71038674 MsaT028955.1:CDS
GAAATATGTTCTCTTGATGC+GGG 0.559456 5:+71037188 None:intergenic
AGAAGTTTCAGGAGTGTGAA+AGG 0.560086 5:-71035060 MsaT028955.1:intron
TGTAGACAAGAACATTTCCA+TGG 0.561347 5:-71037816 MsaT028955.1:CDS
TCATTACGTTTAGTGTCGGA+GGG 0.565562 5:+71034764 None:intergenic
CATGCAAGAGAGAGAAAACT+GGG 0.565706 5:-71036706 MsaT028955.1:CDS
TTTGAGTCATCATCACAGTA+GGG 0.566382 5:+71034609 None:intergenic
AGGATGATACCGCCAGTCCA+TGG 0.569796 5:+71038662 None:intergenic
GAATTGGATTCAACCAGGAC+GGG 0.570273 5:-71037730 MsaT028955.1:CDS
AGAAATATGTTCTCTTGATG+CGG 0.570315 5:+71037187 None:intergenic
TCTACGTGTTACAAGACTAA+AGG 0.576121 5:-71037123 MsaT028955.1:CDS
TGGTTGATATCTAACTTCTG+TGG 0.576289 5:+71038100 None:intergenic
CTTCTGGTGCAGTAATGCTA+TGG 0.580347 5:-71035463 MsaT028955.1:CDS
ATGGTAAACGACGGTACGGA+GGG 0.581370 5:+71036020 None:intergenic
AAATAGAACATTAAAGGACA+TGG 0.581441 5:-71035376 MsaT028955.1:CDS
CCTGAAAGAAGGACAAAGAG+TGG 0.582016 5:-71036048 MsaT028955.1:CDS
AGAACTGCATTCAACCAGAA+GGG 0.582774 5:-71037914 MsaT028955.1:CDS
TAAAAGAAATTGAAAATGGA+CGG 0.583452 5:-71036824 MsaT028955.1:CDS
GAGAATGTAAAAGTTGACTT+CGG 0.584486 5:+71038235 None:intergenic
TAGATATCAACCACAAAAGA+CGG 0.585489 5:-71038090 MsaT028955.1:CDS
GTGTGAAATTCGAGTGAATG+GGG 0.586920 5:-71034308 MsaT028955.1:CDS
TGATAGTGTCTGGGAAGCGA+TGG 0.590031 5:-71035099 MsaT028955.1:CDS
CATGGTAAACGACGGTACGG+AGG 0.590266 5:+71036019 None:intergenic
AAATCAACCAGGATTCTCCA+TGG 0.591749 5:-71038679 MsaT028955.1:CDS
AATGCAATAAATGTGAAAGA+TGG 0.592440 5:-71036926 MsaT028955.1:CDS
CAAACGAGAAGCTGTCACCT+TGG 0.594598 5:+71035503 None:intergenic
CAGAACTGCATTCAACCAGA+AGG 0.597524 5:-71037915 MsaT028955.1:CDS
GAACAGTTTGGATGAGCGCA+TGG 0.601484 5:+71034364 None:intergenic
GATGTAGAGGACAAACAAGA+AGG 0.602481 5:-71037752 MsaT028955.1:CDS
GATATCATTACGTTTAGTGT+CGG 0.603752 5:+71034760 None:intergenic
TGGGAATCAGGAGTCAAATG+AGG 0.603778 5:-71034540 MsaT028955.1:CDS
CAGTATGCCTACTCTCGAAG+AGG 0.611365 5:-71037948 MsaT028955.1:CDS
GGAATTGGATTCAACCAGGA+CGG 0.615506 5:-71037731 MsaT028955.1:CDS
CAGGAATTTGGCTCTGAATG+CGG 0.618780 5:-71036129 MsaT028955.1:CDS
TATTGGTGATGATGGTGAAG+AGG 0.619524 5:-71037864 MsaT028955.1:CDS
GCAGGAGGAATATATGAACC+TGG 0.625899 5:-71038296 MsaT028955.1:intron
ATATATGAACCTGGAGACGT+TGG 0.628232 5:-71038287 MsaT028955.1:CDS
CAGGCTGGCCAAATATCAGT+GGG 0.628731 5:-71037977 MsaT028955.1:CDS
GAACTGCATTCAACCAGAAG+GGG 0.630377 5:-71037913 MsaT028955.1:CDS
AATAGAACATTAAAGGACAT+GGG 0.630593 5:-71035375 MsaT028955.1:CDS
GATGAACACACTTCAAAGAA+CGG 0.631803 5:-71034921 MsaT028955.1:CDS
CAGGATTCTCCATGGACTGG+CGG 0.633302 5:-71038671 MsaT028955.1:intron
ATCTATAAGAGAATTCAGTA+GGG 0.634140 5:+71036346 None:intergenic
ACAGGCTGGCCAAATATCAG+TGG 0.635063 5:-71037978 MsaT028955.1:CDS
AAAGAAATGATCATATACGT+TGG 0.637901 5:+71035551 None:intergenic
ATGGTTCAGCCTTAGTTGCG+CGG 0.638251 5:+71034383 None:intergenic
GCCATGAAGTGATAGTGTCT+GGG 0.641094 5:-71035108 MsaT028955.1:CDS
TCTCATGGTAAACGACGGTA+CGG 0.644785 5:+71036016 None:intergenic
TGCACTCTACAACAAGAAAG+CGG 0.645642 5:-71036889 MsaT028955.1:CDS
ACTCTCGAAGAGGACATACA+AGG 0.646105 5:-71037938 MsaT028955.1:CDS
ATCGAGAGGATGCTTGTTAG+GGG 0.654312 5:-71038771 None:intergenic
AATTAGAGAAGTGCTATCTG+TGG 0.656894 5:-71036424 MsaT028955.1:CDS
CATTACGTTTAGTGTCGGAG+GGG 0.657734 5:+71034765 None:intergenic
GAGAAAGCTCTTGAAAGACA+TGG 0.661125 5:-71037842 MsaT028955.1:CDS
TGGAGGAATACTTGGCACGA+CGG 0.668087 5:+71034657 None:intergenic
AGAATGATAAGCTACGCCGT+TGG 0.671422 5:-71035763 MsaT028955.1:intron
CAGATGGTAAAGGATCATCA+TGG 0.676590 5:+71034637 None:intergenic
AGGGAATGAGAAACTTGATG+AGG 0.676669 5:-71036673 MsaT028955.1:intron
AACTGCATTCAACCAGAAGG+GGG 0.679999 5:-71037912 MsaT028955.1:CDS
AGAAGGAATTGGATTCAACC+AGG 0.685395 5:-71037735 MsaT028955.1:CDS
GCTGAATTAAGAAATCAACC+AGG 0.689385 5:-71038690 MsaT028955.1:CDS
CAGTATCTCATGGTAAACGA+CGG 0.708055 5:+71036011 None:intergenic
ATGGTAAAGGATCATCATGG+AGG 0.738220 5:+71034640 None:intergenic
TGTGAAATTCGAGTGAATGG+GGG 0.740491 5:-71034307 MsaT028955.1:CDS
GTTTCTGGACATCATCCCAG+AGG 0.751856 5:-71036376 MsaT028955.1:CDS
ACACACTTCAAAGAACGGCG+AGG 0.771728 5:-71034916 MsaT028955.1:CDS
AAATTCTGAAGTATTCACCG+AGG 0.788052 5:+71038204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 71033988 71038784 71033988 ID=MsaG028955
Chr5 mRNA 71033988 71038784 71033988 ID=MsaT028955.1;Parent=MsaG028955
Chr5 exon 71033988 71034084 71033988 ID=MsaT028955.1.exon14;Parent=MsaT028955.1
Chr5 CDS 71033988 71034084 71033988 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71034220 71034446 71034220 ID=MsaT028955.1.exon13;Parent=MsaT028955.1
Chr5 CDS 71034220 71034446 71034220 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71034535 71034804 71034535 ID=MsaT028955.1.exon12;Parent=MsaT028955.1
Chr5 CDS 71034535 71034804 71034535 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71034899 71034956 71034899 ID=MsaT028955.1.exon11;Parent=MsaT028955.1
Chr5 CDS 71034899 71034956 71034899 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71035061 71035200 71035061 ID=MsaT028955.1.exon10;Parent=MsaT028955.1
Chr5 CDS 71035061 71035200 71035061 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71035335 71035626 71035335 ID=MsaT028955.1.exon9;Parent=MsaT028955.1
Chr5 CDS 71035335 71035626 71035335 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71035764 71035909 71035764 ID=MsaT028955.1.exon8;Parent=MsaT028955.1
Chr5 CDS 71035764 71035909 71035764 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71036011 71036211 71036011 ID=MsaT028955.1.exon7;Parent=MsaT028955.1
Chr5 CDS 71036011 71036211 71036011 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71036341 71036469 71036341 ID=MsaT028955.1.exon6;Parent=MsaT028955.1
Chr5 CDS 71036341 71036469 71036341 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71036674 71036955 71036674 ID=MsaT028955.1.exon5;Parent=MsaT028955.1
Chr5 CDS 71036674 71036955 71036674 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71037041 71037316 71037041 ID=MsaT028955.1.exon4;Parent=MsaT028955.1
Chr5 CDS 71037041 71037316 71037041 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71037574 71038314 71037574 ID=MsaT028955.1.exon3;Parent=MsaT028955.1
Chr5 CDS 71037574 71038314 71037574 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71038450 71038522 71038450 ID=MsaT028955.1.exon2;Parent=MsaT028955.1
Chr5 CDS 71038450 71038522 71038450 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Chr5 exon 71038672 71038784 71038672 ID=MsaT028955.1.exon1;Parent=MsaT028955.1
Chr5 CDS 71038672 71038784 71038672 ID=cds.MsaT028955.1;Parent=MsaT028955.1
Gene Sequence

>MsaT028955.1

ATGCTTGTTAGGGGTTGTCATTTAGGTGGGACTTCGTCAGTTGTCTCAAACATCTCGCGAAAGAGATGTTTTGCTGAATTAAGAAATCAACCAGGATTCTCCATGGACTGGCGTGTTGAAATCATTCATAATGAGATATTTGCAACTATGCTGGAAATTTCGTTACTTACCAATGCTGCTTCAAAGGAGGAATATATGAACCTGGAGACGTTGGATGAGCGTGTGCAAGCTCTATTGGGTGTTCCGAAGTCAACTTTTACATTCTCACAATCTGCCTCGGTGAATACTTCAGAATTTTCCACTCATGATGGCAACACTGAAGAATTTTCCAATAATTTTCCTGATGTCTGGTCTTGTATAGACTTCAGTGAATATCAACCACAGAAGTTAGATATCAACCACAAAAGACGGAAAATAATGGAGTCTGGTTGTGATGTTTCTTTAGTTAACAATGCAAGTTACAATCAACCAATTCACGAGATAACTCCCTCAAAAGGCTTACAGGCTGGCCAAATATCAGTGGGAATTGACAGTATGCCTACTCTCGAAGAGGACATACAAGGCAGAACTGCATTCAACCAGAAGGGGGTAAATACTAACAGAGAACTTATTATTATTGGTGATGATGGTGAAGAGGAGAAAGCTCTTGAAAGACATGGCATTGTAGACAAGAACATTTCCATGGAAATGGACATTGAAGAAAATTCCGATTTGCATGTTCCTATTGATGTAGAGGACAAACAAGAAGGAATTGGATTCAACCAGGACGGGACAGATGCTCAAAAGGAAGTCAATGAGCCAAAAACTGATCAAGAGAATCACACAAAATCCTCTAATAGAATAAATGATGCTGTTTCATTGATTGATTCGTTCACATGGAAGCAAATAACAGAACATGTTAGTAGTCTGAGAAAGGAATCTGTTCAGATTACTTCTGAGGCTGAAGCACGAATTGATGCGAACACTTGCCAATTATGTGAAAAGGGAAGACTTTACTTTGCACCAGTCCCACTTTTTTGTTTACATTGTGGCACCCGCATCAAGAGAACATATTTCTGTACGAAAGAAGATGATTTTGATGCACAGGGTTGCATTTGCTCTACGTGTTACAAGACTAAAGGTGACAAAATCGCATTCAATGGGACATCTATTTCCAAGAAAAATCTTGAAAAAAGGAATAATGATGAAGTACTTGAAGAACCGTGGGTTGAATGCAATAAATGTGAAAGATGGCAGCATCAAATATGTGCACTCTACAACAAGAAAGCGGATGTGGACAGCAATGCTGAGTATATATGTCCTTTATGCCGCCTAAAAGAAATTGAAAATGGACGGCCTTTTCCCTTACCGAAGGCAGCTAACTTTGGTGCTAAGGACTTGACAAGAACTGTGCTAAGTGACCACTTGGAGAAAAGACTTTTCGAGCGTCTCATGCAAGAGAGAGAAAACTGGGAAAAAGTTGAAGGGAATGAGAAACTTGATGAGGTTATAGCAGCAGAAAATCTTACAATTAGAGAAGTGCTATCTGTGGACAAGCAATTGAAAGTAAATAAGCAGTTTCTGGACATCATCCCAGAGGAGAATTACCCTACTGAATTCTCTTATAGATCAAGAGTTATACTTCTGTTTCAGAAAATTGAAGGAGTAGATGTATGCATTTTTGGAATGTATGTCCAGGAATTTGGCTCTGAATGCGGCAATCCCAATCAACGTTGTGTATACATTTCATATCTTGATTCCGTCAAGTATTTTAGGCCTGAAAGAAGGACAAAGAGTGGAGAAGCCCTCCGTACCGTCGTTTACCATGAGATACTGATCGGATACCTTGATTTTTGCAAGAAAAATGGTTTCACAACTTGCTATATATGGGCCTGTGCTCCTTCAGAAAAAGGGGATGATTATATTCTATATTGCCATCCCAAAGAGCAAAAAACTCCAAAGAATGATAAGCTACGCCGTTGGTATCTTTCAATGCTAAAAAAGGCTACCGAAGAAAATATTGTCGTTGGTTTAACCAACGTATATGATCATTTCTTTCTTCCTGCTACTGAAAAAGGGAACTCCAAGGTGACAGCTTCTCGTTTGCCATACTTTGATGGAGACTTCTGGTGCAGTAATGCTATGGTAGTAGCCAAGACCCTCGAAAAAGAGAGTAGAGGAAACTATGAAAAGTTGTTGAAACAAGTGTCAAATAGAACATTAAAGGACATGGGACATTCCAAGCCTTCCAAAGACATTTTGGTGATGCAAAAAGTTGGCCAAAATATATTGCCTACAAAAGAAAACTTCTTCGTGGCTCACTTGCGATCTTCATGCATACACTGCCATGAAGTGATAGTGTCTGGGAAGCGATGGTTTTGCACTGAATGCAAGAAGTTTCAGGAGTGTGAAAGATGCCATAGTTCTGATGAACACACTTCAAAGAACGGCGAGGTGCATACACTTTGTCAGGCTTCTGTTGATGATATCCCCTCCGACACTAAACGTAATGATATCGTTCTTGAGAGTAGATTGTTTGAAAATAGGGACAACTTTTTGATCTTCTGCCAGAAGTCTCAGTTTCAGTTTGACACACTCCGTCGTGCCAAGTATTCCTCCATGATGATCCTTTACCATCTGCACAACCCTACTGTGATGATGACTCAAAATGAACACCAGTTGACTCAAAATTGTTCAACACCAGTATGTCAATCTGGGAATCAGGAGTCAAATGAGGAAATGATGCTGAAACTGCTGAACATTTTGAAGCATGCATCTCAATGCCGCGCAACTAAGGCTGAACCATGCGCTCATCCAAACTGTTCTCAGATCAAGAAGTTGTTCTCTCATGCCAGTAAGTGTGAAATTCGAGTGAATGGGGGCTGCCAGCATTGTAAGAAGATATGGTTTATACTAACTGCTCACTCCAGAAATTGCAAAGACTCGGAATGTAGAATACCAAGTTGCAGCGATCTGAAGAAGCATGTAGAACAGAAATCAATGCATTCTGAATCTTGTCATAGAGCTAAAGTTTTGGAAGGCGACGGACCAGTCAATGGCCAATGA
Protein sequence

>MsaT028955.1

MLVRGCHLGGTSSVVSNISRKRCFAELRNQPGFSMDWRVEIIHNEIFATMLEISLLTNAASKEEYMNLETLDERVQALLGVPKSTFTFSQSASVNTSEFSTHDGNTEEFSNNFPDVWSCIDFSEYQPQKLDINHKRRKIMESGCDVSLVNNASYNQPIHEITPSKGLQAGQISVGIDSMPTLEEDIQGRTAFNQKGVNTNRELIIIGDDGEEEKALERHGIVDKNISMEMDIEENSDLHVPIDVEDKQEGIGFNQDGTDAQKEVNEPKTDQENHTKSSNRINDAVSLIDSFTWKQITEHVSSLRKESVQITSEAEARIDANTCQLCEKGRLYFAPVPLFCLHCGTRIKRTYFCTKEDDFDAQGCICSTCYKTKGDKIAFNGTSISKKNLEKRNNDEVLEEPWVECNKCERWQHQICALYNKKADVDSNAEYICPLCRLKEIENGRPFPLPKAANFGAKDLTRTVLSDHLEKRLFERLMQERENWEKVEGNEKLDEVIAAENLTIREVLSVDKQLKVNKQFLDIIPEENYPTEFSYRSRVILLFQKIEGVDVCIFGMYVQEFGSECGNPNQRCVYISYLDSVKYFRPERRTKSGEALRTVVYHEILIGYLDFCKKNGFTTCYIWACAPSEKGDDYILYCHPKEQKTPKNDKLRRWYLSMLKKATEENIVVGLTNVYDHFFLPATEKGNSKVTASRLPYFDGDFWCSNAMVVAKTLEKESRGNYEKLLKQVSNRTLKDMGHSKPSKDILVMQKVGQNILPTKENFFVAHLRSSCIHCHEVIVSGKRWFCTECKKFQECERCHSSDEHTSKNGEVHTLCQASVDDIPSDTKRNDIVLESRLFENRDNFLIFCQKSQFQFDTLRRAKYSSMMILYHLHNPTVMMTQNEHQLTQNCSTPVCQSGNQESNEEMMLKLLNILKHASQCRATKAEPCAHPNCSQIKKLFSHASKCEIRVNGGCQHCKKIWFILTAHSRNCKDSECRIPSCSDLKKHVEQKSMHSESCHRAKVLEGDGPVNGQ*