AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG029067

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029067 MtrunA17_Chr5g0440451 89.826 344 17 2 319 655 603 935 0.0 599
MsaG029067 MtrunA17_Chr5g0440451 95.794 214 4 1 1 214 1 209 3.00e-137 424
MsaG029067 MtrunA17_Chr5g0440451 98.276 116 2 0 214 329 418 533 3.81e-70 245
MsaG029067 MtrunA17_Chr3g0118611 67.808 146 46 1 510 654 628 773 1.40e-53 196
MsaG029067 MtrunA17_Chr3g0118611 74.783 115 29 0 214 328 315 429 4.11e-50 186
MsaG029067 MtrunA17_Chr3g0118611 59.200 125 47 2 330 454 518 638 2.77e-33 136
MsaG029067 MtrunA17_Chr3g0118611 59.420 69 27 1 143 210 34 102 1.67e-16 83.6
MsaG029067 MtrunA17_Chr5g0448511 53.279 122 55 1 214 333 209 330 1.68e-33 135
MsaG029067 MtrunA17_Chr5g0448761 48.000 125 63 1 214 336 437 561 1.29e-29 125
MsaG029067 MtrunA17_Chr5g0448761 34.028 144 78 3 47 173 59 202 8.13e-18 87.8
MsaG029067 MtrunA17_Chr1g0207551 39.683 126 73 2 212 335 400 524 2.41e-18 89.7
MsaG029067 MtrunA17_Chr2g0329361 36.066 122 63 1 47 153 60 181 2.87e-18 85.1
MsaG029067 MtrunA17_Chr7g0254601 52.381 84 37 1 510 590 184 267 1.78e-17 83.2
MsaG029067 MtrunA17_Chr7g0233911 34.109 129 66 3 27 136 8 136 4.78e-17 80.5
MsaG029067 MtrunA17_Chr2g0291581 38.655 119 70 2 214 329 426 544 9.01e-17 84.3
MsaG029067 MtrunA17_Chr1g0202341 37.719 114 69 1 215 326 422 535 1.90e-16 83.2
MsaG029067 MtrunA17_Chr3g0078041 40.566 106 49 1 47 138 49 154 4.25e-16 78.2
MsaG029067 MtrunA17_Chr4g0033611 39.216 102 50 2 47 136 75 176 1.81e-15 77.0
MsaG029067 MtrunA17_Chr2g0291591 38.655 119 68 2 214 327 429 547 7.33e-15 77.8
MsaG029067 MtrunA17_Chr3g0080351 37.963 108 65 1 214 319 436 543 1.46e-14 77.4
MsaG029067 MtrunA17_Chr2g0329421 37.255 102 52 2 47 136 26 127 7.17e-14 71.2
MsaG029067 MtrunA17_Chr2g0329031 36.066 122 75 2 214 333 375 495 1.08e-13 74.7
MsaG029067 MtrunA17_Chr6g0477261 38.211 123 72 2 215 333 252 374 9.09e-13 71.6
MsaG029067 MtrunA17_Chr2g0296781 39.655 116 66 2 215 326 363 478 1.36e-12 70.9
MsaG029067 MtrunA17_Chr2g0287061 37.363 91 57 0 46 136 27 117 2.31e-12 70.5
MsaG029067 MtrunA17_Chr2g0287061 40.496 121 70 2 215 335 516 634 4.54e-11 66.2
MsaG029067 MtrunA17_Chr2g0287061 34.783 92 60 0 47 138 182 273 6.56e-11 65.9
MsaG029067 MtrunA17_Chr5g0441581 36.893 103 63 1 215 315 391 493 4.69e-12 69.3
MsaG029067 MtrunA17_Chr1g0196541 37.383 107 62 2 215 319 363 466 2.58e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG029067 AT2G27110.3 69.291 127 38 1 213 338 247 373 2.40e-51 189
MsaG029067 AT2G27110.3 62.329 146 54 1 510 654 560 705 3.36e-48 181
MsaG029067 AT2G27110.3 37.857 280 129 10 325 591 443 690 5.79e-36 144
MsaG029067 AT2G27110.2 69.291 127 38 1 213 338 392 518 4.68e-51 190
MsaG029067 AT2G27110.2 62.329 146 54 1 510 654 705 850 1.16e-47 180
MsaG029067 AT2G27110.2 37.456 283 132 10 325 594 588 838 5.95e-36 145
MsaG029067 AT2G27110.2 37.981 208 113 5 1 202 1 198 1.13e-29 125
MsaG029067 AT2G27110.1 69.291 127 38 1 213 338 392 518 4.68e-51 190
MsaG029067 AT2G27110.1 62.329 146 54 1 510 654 705 850 1.16e-47 180
MsaG029067 AT2G27110.1 37.456 283 132 10 325 594 588 838 5.95e-36 145
MsaG029067 AT2G27110.1 37.981 208 113 5 1 202 1 198 1.13e-29 125
MsaG029067 AT4G38180.1 48.361 122 61 1 214 333 446 567 5.92e-31 129
MsaG029067 AT4G38180.1 32.934 167 94 2 24 173 49 214 9.76e-21 97.4
MsaG029067 AT4G38170.1 47.541 122 62 1 214 333 211 332 3.68e-29 122
MsaG029067 AT4G12850.4 33.594 128 73 2 39 154 2 129 6.42e-18 81.3
MsaG029067 AT4G15090.1 37.008 127 78 1 215 339 423 549 1.02e-17 88.2
MsaG029067 AT4G15090.5 37.008 127 78 1 215 339 423 549 1.02e-17 88.2
MsaG029067 AT4G15090.3 37.008 127 78 1 215 339 423 549 1.02e-17 88.2
MsaG029067 AT4G15090.2 37.008 127 78 1 215 339 423 549 1.02e-17 88.2
MsaG029067 AT4G15090.4 37.008 127 78 1 215 339 423 549 1.02e-17 88.2
MsaG029067 AT4G12850.2 35.185 108 60 1 39 136 2 109 1.09e-17 80.5
MsaG029067 AT5G18960.2 39.604 101 61 0 47 147 42 142 1.50e-17 87.4
MsaG029067 AT5G18960.2 34.337 166 105 3 215 380 544 705 1.16e-15 81.3
MsaG029067 AT5G18960.2 33.628 113 71 1 24 136 191 299 1.21e-12 71.6
MsaG029067 AT5G18960.1 39.604 101 61 0 47 147 42 142 1.50e-17 87.4
MsaG029067 AT5G18960.1 34.337 166 105 3 215 380 544 705 1.16e-15 81.3
MsaG029067 AT5G18960.1 33.628 113 71 1 24 136 191 299 1.21e-12 71.6
MsaG029067 AT3G59470.3 34.899 149 75 4 8 144 39 177 1.77e-17 83.2
MsaG029067 AT3G59470.1 34.899 149 75 4 8 144 39 177 1.77e-17 83.2
MsaG029067 AT3G59470.2 34.899 149 75 4 8 144 51 189 2.39e-17 82.8
MsaG029067 AT4G12850.1 35.185 108 60 1 39 136 2 109 1.24e-16 79.0
MsaG029067 AT3G07500.1 38.835 103 50 2 47 136 32 134 5.32e-16 77.8
MsaG029067 AT3G06250.2 39.560 91 55 0 47 137 27 117 7.27e-16 82.0
MsaG029067 AT3G06250.2 35.115 131 84 1 209 339 514 643 1.03e-14 78.2
MsaG029067 AT3G06250.2 35.417 96 62 0 41 136 183 278 2.00e-12 70.9
MsaG029067 AT3G06250.1 39.560 91 55 0 47 137 27 117 7.27e-16 82.0
MsaG029067 AT3G06250.1 35.115 131 84 1 209 339 514 643 1.03e-14 78.2
MsaG029067 AT3G06250.1 35.417 96 62 0 41 136 183 278 2.00e-12 70.9
MsaG029067 AT3G06250.3 39.560 91 55 0 47 137 27 117 7.27e-16 82.0
MsaG029067 AT3G06250.3 35.115 131 84 1 209 339 514 643 1.03e-14 78.2
MsaG029067 AT3G06250.3 35.417 96 62 0 41 136 183 278 2.00e-12 70.9
MsaG029067 AT2G43280.1 38.679 106 51 1 47 138 21 126 1.96e-15 75.9
MsaG029067 AT1G52520.1 36.667 120 72 3 214 329 442 561 2.89e-14 76.6
MsaG029067 AT3G22170.2 30.328 122 83 1 214 333 431 552 2.18e-12 70.9
MsaG029067 AT3G22170.1 30.328 122 83 1 214 333 431 552 2.18e-12 70.9
MsaG029067 AT1G80010.1 32.520 123 79 3 215 333 468 590 3.22e-12 70.1
MsaG029067 AT1G76320.1 35.246 122 77 1 214 333 359 480 4.42e-12 69.7
MsaG029067 AT1G76320.4 35.246 122 77 1 214 333 359 480 4.42e-12 69.7
MsaG029067 AT1G76320.2 35.246 122 77 1 214 333 359 480 4.53e-12 69.7
MsaG029067 AT1G76320.3 35.246 122 77 1 214 333 359 480 6.71e-12 69.3
MsaG029067 AT4G19990.6 37.815 119 71 2 214 329 370 488 4.00e-11 66.6
MsaG029067 AT4G19990.2 37.815 119 71 2 214 329 370 488 4.00e-11 66.6
MsaG029067 AT4G19990.1 37.815 119 71 2 214 329 313 431 4.51e-11 66.6
MsaG029067 AT4G19990.3 37.815 119 71 2 214 329 370 488 5.10e-11 66.6
MsaG029067 AT4G19990.4 37.815 119 71 2 214 329 370 488 5.10e-11 66.6
MsaG029067 AT4G19990.5 37.815 119 71 2 214 329 370 488 5.51e-11 66.2
MsaG029067 AT4G19990.7 37.815 119 71 2 214 329 384 502 5.96e-11 66.2

Find 176 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATAATGCCCGGGTTTC+TGG 0.076489 5:-72536884 MsaT029067.1:CDS
CCCAGTCCTTTGTAAGTTTC+AGG 0.139099 5:+72540843 None:intergenic
ATGGGTTGCCTCACATTATA+AGG 0.229489 5:+72540687 None:intergenic
ACTTTCAACAACAGCAAATA+AGG 0.298234 5:-72538114 MsaT029067.1:CDS
CAACATTACCAATGTTCTTT+AGG 0.301189 5:-72539931 MsaT029067.1:CDS
CATAATGTGGATGCTGAATT+TGG 0.311841 5:-72541269 MsaT029067.1:CDS
TTAACCTCATATTCTGTTGA+TGG 0.333158 5:+72538200 None:intergenic
GTGTCTCGTCAGCGAGTTGA+TGG 0.365862 5:-72538999 MsaT029067.1:CDS
CGGCATATCTGATGGCTTCC+CGG 0.375029 5:+72539414 None:intergenic
GGCGAAGGTGTCTTTAGAAC+TGG 0.389519 5:+72539836 None:intergenic
TCAACTCGCTGACGAGGCAC+TGG 0.390203 5:+72539068 None:intergenic
CAACTCGCTGACGAGGCATT+GGG 0.391167 5:+72539036 None:intergenic
CGTGCCACTGGGGTCGTTTG+TGG 0.394528 5:+72539113 None:intergenic
AGATCAGCCATTGGTTGAAC+AGG 0.395974 5:+72539143 None:intergenic
TACTCTGTTCTCTGGATTTG+AGG 0.396619 5:+72538480 None:intergenic
ACCTTCGCCAATGGAGAATC+AGG 0.397585 5:-72539822 MsaT029067.1:CDS
TCATGCTGAGGGTATGGTTT+CGG 0.402062 5:-72541216 MsaT029067.1:CDS
AGCAAAAGGGTCAAAATAAG+TGG 0.402254 5:-72540971 MsaT029067.1:CDS
CATCTCGCTGACGAGGCATT+GGG 0.408269 5:+72538937 None:intergenic
CTGACAATAGTCAAAAGATT+AGG 0.415523 5:-72540767 MsaT029067.1:CDS
TGATGGGACGAATATGTTCC+GGG 0.418146 5:-72541063 MsaT029067.1:CDS
TGTATAGTGCTCGTGATCAA+TGG 0.420791 5:-72540048 MsaT029067.1:CDS
ATCCTCTTCGATTCTGTTGG+CGG 0.420813 5:+72539730 None:intergenic
AAGTTAAGTTTCAAGTATCC+AGG 0.425548 5:-72536853 MsaT029067.1:CDS
TCATCTTGCTGAAGAGGCGC+TGG 0.427906 5:+72538903 None:intergenic
TGAAGTAGGGGTTCTCTTGT+CGG 0.428439 5:+72539247 None:intergenic
TCATCTCGCTGACGAGGCAT+TGG 0.429820 5:+72538936 None:intergenic
TCAACTTGCTTACGTGCCAC+TGG 0.432157 5:+72539101 None:intergenic
CCCATCCTCTTCGATTCTGT+TGG 0.437674 5:+72539727 None:intergenic
ATGAACTAGCTCTGGAGAAA+TGG 0.440287 5:-72539904 MsaT029067.1:CDS
AAAGTTGCGAGGCGATGATT+AGG 0.441916 5:-72540998 MsaT029067.1:CDS
TCCGGGAGTTTGTGTGTAGC+AGG 0.444056 5:-72541046 MsaT029067.1:CDS
AGGTTAAGTTCCATCTCTCT+CGG 0.446028 5:-72538184 MsaT029067.1:CDS
GATGATTAGGATAGAGCAAA+AGG 0.447571 5:-72540985 MsaT029067.1:CDS
CACGGTATGCCAATCTGTGC+CGG 0.447645 5:-72539433 MsaT029067.1:CDS
TCAACTCGCTGACGAGGCAT+TGG 0.448513 5:+72539035 None:intergenic
CTGATGGGACGAATATGTTC+CGG 0.449113 5:-72541064 MsaT029067.1:CDS
TCAAGTATCCAGGGATGCAT+TGG 0.450589 5:-72536843 MsaT029067.1:CDS
CATCTTGCTGAAGAGGCGCT+GGG 0.450975 5:+72538904 None:intergenic
TGAAGTCTATGACTGAAGTC+GGG 0.455020 5:-72538142 MsaT029067.1:CDS
TTCTCTGGCGGTACCTGATA+AGG 0.456816 5:-72540916 MsaT029067.1:CDS
CGCTGACGAGGCACTGGGGA+TGG 0.458341 5:+72539074 None:intergenic
TGTCTCAGCTTGATAATGCC+CGG 0.459217 5:-72536892 MsaT029067.1:intron
GTCAATGTTTCATCGTTAAG+TGG 0.460945 5:+72539191 None:intergenic
CGCCTACGAGGCACTGGTCG+TGG 0.461327 5:+72538975 None:intergenic
GCAGGGAAGGTTTGAAAAGA+AGG 0.461483 5:-72541028 MsaT029067.1:CDS
CACAGCACCCAATGCATCCC+TGG 0.463515 5:+72536835 None:intergenic
GAAGTCAATGACGTGGGTAA+TGG 0.464484 5:-72538512 MsaT029067.1:CDS
TCAACTCGCTGACGAGACAC+TGG 0.466795 5:+72539002 None:intergenic
CATGGTCAGTTTAATGCTGA+TGG 0.469007 5:-72541080 MsaT029067.1:CDS
GTGCCTCGTAGGCGAGTTGA+TGG 0.469828 5:-72538966 MsaT029067.1:CDS
ACAACGGCTACTCTGTTCTC+TGG 0.470800 5:+72538472 None:intergenic
GAAGTAGGGGTTCTCTTGTC+GGG 0.472681 5:+72539248 None:intergenic
TCAAATACCATGCCCAAATA+CGG 0.473294 5:+72541176 None:intergenic
GGACTGGGGCTTGTTCCAAC+TGG 0.474446 5:-72540828 MsaT029067.1:CDS
GCCTGAAACTTACAAAGGAC+TGG 0.476526 5:-72540844 MsaT029067.1:CDS
AGCTCAAAAGCTGGTTTGCA+TGG 0.476550 5:-72541098 MsaT029067.1:CDS
GGGATCTCATGCTGAGGGTA+TGG 0.478915 5:-72541222 MsaT029067.1:CDS
GTATAGTGCTCGTGATCAAT+GGG 0.479907 5:-72540047 MsaT029067.1:CDS
CTTGTATCTGATGTTGAAGT+AGG 0.481986 5:+72539233 None:intergenic
CAACTAATCATCGACTCAGC+TGG 0.482359 5:-72539286 MsaT029067.1:CDS
CAAGTATCCAGGGATGCATT+GGG 0.482761 5:-72536842 MsaT029067.1:CDS
AGTGAGTATGCTAGACGGTC+GGG 0.482860 5:-72541125 MsaT029067.1:CDS
TTTGGTCAAGTTAATGATGC+TGG 0.483303 5:-72541251 MsaT029067.1:CDS
CGCTGACGAGGCATTGGGGA+TGG 0.486572 5:+72538942 None:intergenic
ATGATTAGTTGATGACACTT+TGG 0.490968 5:+72539298 None:intergenic
CATAGCCACAGCACCTTCCT+CGG 0.491838 5:+72539394 None:intergenic
ACACTAGAAAAGTGCTTCAC+TGG 0.492664 5:+72540879 None:intergenic
TCAGTCATAGACTTCAAAAC+AGG 0.492669 5:+72538149 None:intergenic
TTGAAGTCTATGACTGAAGT+CGG 0.493526 5:-72538143 MsaT029067.1:CDS
TTGTATCTGATGTTGAAGTA+GGG 0.495979 5:+72539234 None:intergenic
TCTGGAGAAATGGTTTGAAA+AGG 0.496885 5:-72539894 MsaT029067.1:CDS
ACTTATGATGCTGCAATGAC+TGG 0.497681 5:-72539368 MsaT029067.1:CDS
CGCTGACGAGGCATTGGGGA+TGG 0.497742 5:+72539041 None:intergenic
CAACTTGCTTACGTGCCACT+GGG 0.498429 5:+72539102 None:intergenic
TCCTTTGTAAGTTTCAGGCA+TGG 0.500821 5:+72540848 None:intergenic
CACGTCATTGACTTCAAAGA+TGG 0.501878 5:+72538520 None:intergenic
GAGTCCATCAACAGAATATG+AGG 0.503904 5:-72538204 MsaT029067.1:CDS
GATGGCTTCCCGGCACAGAT+TGG 0.505149 5:+72539424 None:intergenic
AATGAATCACGGAAATATGC+TGG 0.507187 5:+72540022 None:intergenic
CGCTGACGAGACACTGGGGA+TGG 0.508209 5:+72539008 None:intergenic
GAAGGAACATAGCCATTCTC+TGG 0.512692 5:-72540931 MsaT029067.1:CDS
GTTAATGATGCTGGAAACTC+GGG 0.514570 5:-72541242 MsaT029067.1:CDS
AGTTAATGATGCTGGAAACT+CGG 0.515040 5:-72541243 MsaT029067.1:CDS
ATGCCTCGTCAGCGAGATGA+TGG 0.517544 5:-72538933 MsaT029067.1:CDS
TTCGGAGCCGTATTTGGGCA+TGG 0.517562 5:-72541183 MsaT029067.1:CDS
CTTCAGACTCGCCAGAAACC+CGG 0.518277 5:+72536873 None:intergenic
TAGGCAGTATGAACTAGCTC+TGG 0.519286 5:-72539912 MsaT029067.1:CDS
CGTTAAGTGGCCATAAAAGA+GGG 0.519299 5:+72539204 None:intergenic
GATATATACATGACACCAGT+TGG 0.519725 5:+72540813 None:intergenic
GGGTGCTGTGCTACGATCAA+TGG 0.520286 5:-72536822 MsaT029067.1:CDS
CCTGAAACTTACAAAGGACT+GGG 0.522711 5:-72540843 MsaT029067.1:CDS
CAACTCGCTGACGAGGCACT+GGG 0.522831 5:+72539069 None:intergenic
TCGGGTTTCAGCTCAAAAGC+TGG 0.523459 5:-72541107 MsaT029067.1:CDS
AGGGGTTCTCTTGTCGGGAT+CGG 0.524735 5:+72539253 None:intergenic
GATGTTCATGTGATAACTAT+CGG 0.525824 5:-72541314 MsaT029067.1:CDS
TTAGCAACCTGATTCTCCAT+TGG 0.527218 5:+72539815 None:intergenic
TATCAGGTACCGCCAGAGAA+TGG 0.527369 5:+72540919 None:intergenic
GCGCCTCTTCAGCAAGATGA+TGG 0.535172 5:-72538900 MsaT029067.1:CDS
ATAACTATCGGAGAAGGAAG+TGG 0.536867 5:-72541302 MsaT029067.1:CDS
GAGTTTGTGTGTAGCAGGGA+AGG 0.537943 5:-72541041 MsaT029067.1:CDS
TCGTTAAGTGGCCATAAAAG+AGG 0.538091 5:+72539203 None:intergenic
TCTTGCTGAAGAGGCGCTGG+GGG 0.539141 5:+72538906 None:intergenic
CCATCTCTCTCGGGTTACTG+TGG 0.539645 5:-72538174 MsaT029067.1:CDS
GGTTAAGTTCCATCTCTCTC+GGG 0.539950 5:-72538183 MsaT029067.1:CDS
TCAACTCGCCTACGAGGCAC+TGG 0.539984 5:+72538969 None:intergenic
ACCTTCCTCGGCATATCTGA+TGG 0.541562 5:+72539406 None:intergenic
CCCTGCTACACACAAACTCC+CGG 0.542869 5:+72541045 None:intergenic
CATCTCTCTCGGGTTACTGT+GGG 0.544255 5:-72538173 MsaT029067.1:CDS
GGTAAATTCAGATCAGCCAT+TGG 0.544409 5:+72539134 None:intergenic
GTCTCAGCTTGATAATGCCC+GGG 0.544869 5:-72536891 MsaT029067.1:intron
CGATGCTCCTGTTCAACCAA+TGG 0.548780 5:-72539150 MsaT029067.1:CDS
CGAGGAAGGTGCTGTGGCTA+TGG 0.550729 5:-72539393 MsaT029067.1:CDS
TAGTGAGTATGCTAGACGGT+CGG 0.552667 5:-72541126 MsaT029067.1:CDS
GGACCATCATCTTGCTGAAG+AGG 0.556157 5:+72538897 None:intergenic
ACGGTATGCCAATCTGTGCC+GGG 0.557330 5:-72539432 MsaT029067.1:CDS
CAACTCGCTGACGAGACACT+GGG 0.557677 5:+72539003 None:intergenic
TTCAGACTCGCCAGAAACCC+GGG 0.560618 5:+72536874 None:intergenic
ATCTTGCTGAAGAGGCGCTG+GGG 0.561388 5:+72538905 None:intergenic
TCTAAAGACACCTTCGCCAA+TGG 0.564758 5:-72539831 MsaT029067.1:CDS
CAACAGAATCGAAGAGGATG+GGG 0.566080 5:-72539726 MsaT029067.1:intron
CCAACAGAATCGAAGAGGAT+GGG 0.566509 5:-72539727 MsaT029067.1:CDS
CGGAGAAGGAAGTGGATCGA+TGG 0.574177 5:-72541294 MsaT029067.1:CDS
CCGGGAGTTTGTGTGTAGCA+GGG 0.574471 5:-72541045 MsaT029067.1:CDS
ACCTGATTCTCCATTGGCGA+AGG 0.577117 5:+72539821 None:intergenic
GGTTGTTACAAAATTTGTGA+AGG 0.577403 5:-72540949 MsaT029067.1:CDS
GCCAACAGAATCGAAGAGGA+TGG 0.578654 5:-72539728 MsaT029067.1:CDS
GCTGTAATATCTCCTACTCA+AGG 0.581429 5:-72539995 MsaT029067.1:CDS
CCACAGTAACCCGAGAGAGA+TGG 0.582321 5:+72538174 None:intergenic
GAGGCTTCAAATCCTTGAGT+AGG 0.582780 5:+72539983 None:intergenic
ATGACTGGTCTTAGAGAAGG+TGG 0.586437 5:-72539353 MsaT029067.1:CDS
CATCTTTGAAGTCAATGACG+TGG 0.586514 5:-72538519 MsaT029067.1:CDS
GCCATCAGATATGCCGAGGA+AGG 0.587262 5:-72539407 MsaT029067.1:CDS
TGTATCTGATGTTGAAGTAG+GGG 0.588892 5:+72539235 None:intergenic
GGTTGCTGTAATAAATCTCA+AGG 0.589532 5:-72538093 MsaT029067.1:intron
AACTCGGGATCTCATGCTGA+GGG 0.600343 5:-72541227 MsaT029067.1:CDS
CATATGAGTCTTTGACATCA+CGG 0.600661 5:-72539451 MsaT029067.1:intron
AGTTAAGTTTCAAGTATCCA+GGG 0.603293 5:-72536852 MsaT029067.1:CDS
TTGATCACGAGCACTATACA+AGG 0.604276 5:+72540051 None:intergenic
TGGATCGATGGAACATAATG+TGG 0.607966 5:-72541282 MsaT029067.1:CDS
GCAAAAGGGTCAAAATAAGT+GGG 0.609981 5:-72540970 MsaT029067.1:CDS
GGACTCTCAACTCGCTGACG+AGG 0.611590 5:+72539062 None:intergenic
ATGGTCAGTTTAATGCTGAT+GGG 0.615272 5:-72541079 MsaT029067.1:CDS
AGTAGCCGTTGTCAATCTCA+AGG 0.616717 5:-72538461 MsaT029067.1:intron
CATGTGATAACTATCGGAGA+AGG 0.618752 5:-72541308 MsaT029067.1:CDS
ATCTTTGAAGTCAATGACGT+GGG 0.619370 5:-72538518 MsaT029067.1:CDS
TCATACTGCCTAAAGAACAT+TGG 0.619859 5:+72539923 None:intergenic
GAAGTCTATGACTGAAGTCG+GGG 0.631820 5:-72538141 MsaT029067.1:CDS
GGACCATCATCTCGCTGACG+AGG 0.632084 5:+72538930 None:intergenic
ATGATTAGGATAGAGCAAAA+GGG 0.632703 5:-72540984 MsaT029067.1:CDS
GGACTATCAACTCGCTGACG+AGG 0.634516 5:+72539029 None:intergenic
TACCGCCAACAGAATCGAAG+AGG 0.639324 5:-72539732 MsaT029067.1:CDS
CACATTATAAGGCATCGAAG+TGG 0.639643 5:+72540698 None:intergenic
TCTGGCGGTACCTGATAAGG+TGG 0.640782 5:-72540913 MsaT029067.1:CDS
ATATGCCGAGGAAGGTGCTG+TGG 0.643843 5:-72539399 MsaT029067.1:CDS
ATCTCGCTGACGAGGCATTG+GGG 0.645015 5:+72538938 None:intergenic
TGATGGTCCACCTGAAAACA+CGG 0.645985 5:-72538883 MsaT029067.1:intron
ACATTGGTAATGTTGTATGC+CGG 0.647842 5:+72539939 None:intergenic
GCAATGACTGGTCTTAGAGA+AGG 0.648703 5:-72539356 MsaT029067.1:CDS
GTCCACGACCAGTGCCTCGT+AGG 0.650635 5:-72538977 MsaT029067.1:CDS
TTTATAGTGAGTATGCTAGA+CGG 0.652904 5:-72541130 MsaT029067.1:CDS
AACTCGCTGACGAGGCATTG+GGG 0.657743 5:+72539037 None:intergenic
GGAAGCCATCAGATATGCCG+AGG 0.662402 5:-72539411 MsaT029067.1:CDS
AAACTCGGGATCTCATGCTG+AGG 0.664329 5:-72541228 MsaT029067.1:CDS
ACCATGCCTGAAACTTACAA+AGG 0.669726 5:-72540849 MsaT029067.1:CDS
AACTTGCTTACGTGCCACTG+GGG 0.680413 5:+72539103 None:intergenic
GGAACATAGCCATTCTCTGG+CGG 0.681713 5:-72540928 MsaT029067.1:CDS
AAGGATGACTGAAAGTTGCG+AGG 0.682372 5:-72541009 MsaT029067.1:CDS
GGACCATCAACTCGCCTACG+AGG 0.699625 5:+72538963 None:intergenic
AACTCGCTGACGAGACACTG+GGG 0.704142 5:+72539004 None:intergenic
CAGCACAAGAAAGTTGCTCG+CGG 0.708596 5:+72536792 None:intergenic
AACTCGCTGACGAGGCACTG+GGG 0.710997 5:+72539070 None:intergenic
GTTAAGTGGCCATAAAAGAG+GGG 0.718319 5:+72539205 None:intergenic
ACATACCTTGAGATTGACAA+CGG 0.720523 5:+72538456 None:intergenic
CTGAAACTTACAAAGGACTG+GGG 0.731403 5:-72540842 MsaT029067.1:CDS
TTTACCACAAACGACCCCAG+TGG 0.755041 5:-72539117 MsaT029067.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr5 gene 72536295 72541339 72536295 ID=MsaG029067
Chr5 mRNA 72536295 72541339 72536295 ID=MsaT029067.1;Parent=MsaG029067
Chr5 exon 72536295 72536339 72536295 ID=MsaT029067.1.exon7;Parent=MsaT029067.1
Chr5 CDS 72536295 72536339 72536295 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72536793 72536906 72536793 ID=MsaT029067.1.exon6;Parent=MsaT029067.1
Chr5 CDS 72536793 72536906 72536793 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72538094 72538243 72538094 ID=MsaT029067.1.exon5;Parent=MsaT029067.1
Chr5 CDS 72538094 72538243 72538094 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72538462 72538551 72538462 ID=MsaT029067.1.exon4;Parent=MsaT029067.1
Chr5 CDS 72538462 72538551 72538462 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72538884 72539465 72538884 ID=MsaT029067.1.exon3;Parent=MsaT029067.1
Chr5 CDS 72538884 72539465 72538884 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72539727 72540074 72539727 ID=MsaT029067.1.exon2;Parent=MsaT029067.1
Chr5 CDS 72539727 72540074 72539727 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Chr5 exon 72540701 72541339 72540701 ID=MsaT029067.1.exon1;Parent=MsaT029067.1
Chr5 CDS 72540701 72541339 72540701 ID=cds.MsaT029067.1;Parent=MsaT029067.1
Gene Sequence

>MsaT029067.1

ATGGATGTTCATGTGATAACTATCGGAGAAGGAAGTGGATCGATGGAACATAATGTGGATGCTGAATTTGGTCAAGTTAATGATGCTGGAAACTCGGGATCTCATGCTGAGGGTATGGTTTCGGAGCCATATTTTTCGGAGCCGTATTTGGGCATGGTATTTGATTCTGAAGATTCTGCTAAAAGTTTTTATAGTGAGTATGCTAGACGGTCGGGTTTCAGCTCAAAAGCTGGTTTGCATGGTCAGTTTAATGCTGATGGGACGAATATGTTCCGGGAGTTTGTGTGTAGCAGGGAAGGTTTGAAAAGAAGGATGACTGAAAGTTGCGAGGCGATGATTAGGATAGAGCAAAAGGGTCAAAATAAGTGGGTTGTTACAAAATTTGTGAAGGAACATAGCCATTCTCTGGCGGTACCTGATAAGGTGGAAAAGCTTCAACCAGTGAAGCACTTTTCTAGTGTTGGAAGAACCATGCCTGAAACTTACAAAGGACTGGGGCTTGTTCCAACTGGTGTCATGTATATATCTATTGATGCGCATCACGTTTCCTCTGACAATAGTCAAAAGATTAGGAATTCTCCTGCAGCTGCCGCGAAAACGAGTCATCCAGTTAAAAATTCCACTTCGATGCCTTATAATTCCTTGTATAGTGCTCGTGATCAATGGGTTCCAGCATATTTCCGTGATTCATTTTTCGCTGTAATATCTCCTACTCAAGGATTTGAAGCCTCTTTTTTGGACGGCATTGTCAACCGGCATACAACATTACCAATGTTCTTTAGGCAGTATGAACTAGCTCTGGAGAAATGGTTTGAAAAGGAAATAGAATCAGATTTTGAGACAATTAGCACTAATCCAGTTCTAAAGACACCTTCGCCAATGGAGAATCAGGTTGCTAAGCTTTATACACAGAGAATATTTTTGAAGTTTCAAGAAGAGCTAGTTGAAACTTTTGCTTATACCGCCAACAGAATCGAAGAGGATGGGTCTTTGACATCACGGTATGCCAATCTGTGCCGGGAAGCCATCAGATATGCCGAGGAAGGTGCTGTGGCTATGGAAACTTATGATGCTGCAATGACTGGTCTTAGAGAAGGTGGAAAGAAAGTTGATGTCATGAAGAGAAATATTGCCAAAGTGTCATCAACTAATCATCGACTCAGCTGGAATGATTGCTCCGATCCCGACAAGAGAACCCCTACTTCAACATCAGATACAAGTACAACCCCTCTTTTATGGCCACTTAACGATGAAACATTGACGTTTAATCTTAATGACGTCGATGCTCCTGTTCAACCAATGGCTGATCTGAATTTACCACAAACGACCCCAGTGGCACGTAAGCAAGTTGATGTTCCATCCCCAGTGCCTCGTCAGCGAGTTGAGAGTCCATCCCCAATGCCTCGTCAGCGAGTTGATAGTCCATCCCCAGTGTCTCGTCAGCGAGTTGATGGTCCACGACCAGTGCCTCGTAGGCGAGTTGATGGTCCATCCCCAATGCCTCGTCAGCGAGATGATGGTCCGCCCCCAGCGCCTCTTCAGCAAGATGATGGTCCACCTGAAAACACGGTGGCTCTTCCATCTTTGAAGTCAATGACGTGGGTAATGGAGAACAAAACCTCAAATCCAGAGAACAGAGTAGCCGTTGTCAATCTCAAGTTGCAAGATTACAACAAGAGTCCATCAACAGAATATGAGGTTAAGTTCCATCTCTCTCGGGTTACTGTGGGACCTGTTTTGAAGTCTATGACTGAAGTCGGGGAACAACTTTCAACAACAGCAAATAAGGTTGCTGTAATAAATCTCAAGCTTGATAATGCCCGGGTTTCTGGCGAGTCTGAAGTTAAGTTTCAAGTATCCAGGGATGCATTGGGTGCTGTGCTACGATCAATGGCTTATATCCGCGAGCAACTTTCTTGTGCTGGCGACGCGAACTCAGAACCTATGTCAAAAAAGCGTCGAAACTGA
Protein sequence

>MsaT029067.1

MDVHVITIGEGSGSMEHNVDAEFGQVNDAGNSGSHAEGMVSEPYFSEPYLGMVFDSEDSAKSFYSEYARRSGFSSKAGLHGQFNADGTNMFREFVCSREGLKRRMTESCEAMIRIEQKGQNKWVVTKFVKEHSHSLAVPDKVEKLQPVKHFSSVGRTMPETYKGLGLVPTGVMYISIDAHHVSSDNSQKIRNSPAAAAKTSHPVKNSTSMPYNSLYSARDQWVPAYFRDSFFAVISPTQGFEASFLDGIVNRHTTLPMFFRQYELALEKWFEKEIESDFETISTNPVLKTPSPMENQVAKLYTQRIFLKFQEELVETFAYTANRIEEDGSLTSRYANLCREAIRYAEEGAVAMETYDAAMTGLREGGKKVDVMKRNIAKVSSTNHRLSWNDCSDPDKRTPTSTSDTSTTPLLWPLNDETLTFNLNDVDAPVQPMADLNLPQTTPVARKQVDVPSPVPRQRVESPSPMPRQRVDSPSPVSRQRVDGPRPVPRRRVDGPSPMPRQRDDGPPPAPLQQDDGPPENTVALPSLKSMTWVMENKTSNPENRVAVVNLKLQDYNKSPSTEYEVKFHLSRVTVGPVLKSMTEVGEQLSTTANKVAVINLKLDNARVSGESEVKFQVSRDALGAVLRSMAYIREQLSCAGDANSEPMSKKRRN*